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https://www.readbyqxmd.com/read/28743008/wastewater-contamination-in-antarctic-melt-water-streams-evidenced-by-virological-and-organic-molecular-markers
#1
L F L Tort, K Iglesias, C Bueno, A Lizasoain, M Salvo, J Cristina, N Kandratavicius, L Pérez, R Figueira, M C Bícego, S Taniguchi, N Venturini, E Brugnoli, R Colina, M Victoria
Human activities in the Antarctica including tourism and scientific research have been raised substantially in the last century with the concomitant impact on the Antarctic ecosystems through the release of wastewater mainly from different scientific stations activities. The aim of this study was to assess the wastewater contamination of surface waters and sediments of three melt-water streams (11 sites) by leaking septic tanks located in the vicinity of the Uruguayan Scientific Station in the Fildes Peninsula, King George Island, Antarctica, during summer 2015...
July 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28742795/cancer-associated-fibroblasts-promote-bone-invasion-in-oral-squamous-cell-carcinoma
#2
A A Elmusrati, A E Pilborough, S A Khurram, D W Lambert
BACKGROUND: The molecular mechanisms involved in the invasion of bone by oral squamous cell carcinomas (OSCC) are poorly understood, and little is known about the role of cancer-associated fibroblasts (CAF), the presence of which confers a poor prognosis. METHODS: Clinicopathological data from 277 OSCC cases involving bone resections were reviewed, and 32 cases thoroughly analysed histologically. Immunohistochemistry was used to examine αSMA, RANKL and OPG. Western blotting and qPCR were used to assess myofibroblast (CAF-like) differentiation, RANKL and OPG expression in vitro, and RANKL secretion was analysed by ELISA...
July 25, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28742629/noonan-syndrome-an-underestimated-cause-of-severe-to-profound-sensorineural-hearing-impairment-which-clues-to-suspect-the-diagnosis
#3
Alban Ziegler, Natalie Loundon, Laurence Jonard, Hélène Cavé, Geneviève Baujat, Souad Gherbi, Vincent Couloigner, Sandrine Marlin
OBJECTIVE: To highlight Noonan syndrome as a clinically recognizable cause of severe to profound sensorineural hearing impairment. STUDY DESIGN: New clinical cases and review. SETTING: Patients evaluated for etiological diagnosis by a medical geneticist in a reference center for hearing impairment. PATIENTS: Five patients presenting with confirmed Noonan syndrome and profound sensorineural hearing impairment. INTERVENTIONS: Diagnostic and review of the literature...
July 24, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28742422/virus-isolation-and-molecular-detection-of-bluetongue-and-epizootic-hemorrhagic-disease-viruses-from-naturally-infected-white-tailed-deer-odocoileus-virginianus
#4
Clara Kienzle, Rebecca L Poulson, Mark G Ruder, David E Stallknecht
Hemorrhagic disease in North America is caused by multiple serotypes of epizootic hemorrhagic disease virus (EHDV) and bluetongue virus (BTV). Diagnostic tests for detection of EHDV and BTV include virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and real-time RT-PCR (rRT-PCR). Our objective was to compare the diagnostic capabilities of three rRT-PCR protocols for detection of EHDV and BTV from naturally infected white-tailed deer (Odocoileus virginianus). We compared the effectiveness of these assays to traditional viral detection methods (e...
July 25, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28741255/genetics-of-schizophrenia-ready-to-translate
#5
REVIEW
Claire Foley, Aiden Corvin, Shigeki Nakagome
PURPOSE OF REVIEW: This is an era where we have significantly advanced the understanding of the genetic architecture of schizophrenia. In this review, we consider how this knowledge may translate into advances that will improve patient care. RECENT FINDINGS: Large-scale genome-wide association studies (GWAS) have identified more than a hundred loci each making a small contribution to illness risk. Meta-analysis of copy number variants (CNVs) in the Psychiatric Genomics Consortium (PGC) dataset has confirmed that some variants have a moderate or large impact on risk, although these are rare in the population...
September 2017: Current Psychiatry Reports
https://www.readbyqxmd.com/read/28741076/detection-of-verticillium-species-in-swedish-soils-using-real-time-pcr
#6
Georgios Tzelepis, Sarosh Bejai, Muhammad Naeem Sattar, Arne Schwelm, Jonas Ilbäck, Johan Fogelqvist, Christina Dixelius
Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V...
July 24, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28741041/end-of-an-enigma-aenigmopteris-belongs-in-tectaria-tectariaceae-polypodiopsida
#7
Cheng-Wei Chen, Carl J Rothfels, Andi Maryani A Mustapeng, Markus Gubilil, Dirk Nikolaus Karger, Michael Kessler, Yao-Moan Huang
The phylogenetic affinities of the fern genus Aenigmopteris have been the subject of considerable disagreement, but until now, no molecular data were available from the genus. Based on the analysis of three chloroplast DNA regions (rbcL, rps16-matK, and trnL-F) we demonstrate that Aenigmopteris dubia (the type species of the genus) and A. elegans are closely related and deeply imbedded in Tectaria. The other three species of genus are morphologically very similar; we therefore transfer all five known species into Tectaria...
July 24, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28740553/a-fluorogenic-probe-for-ultrafast-and-reversible-detection-of-formaldehyde-in-neurovascular-tissues
#8
Xing-Guang Liang, Bo Chen, Ling-Xiao Shao, Juan Cheng, Ming-Zhu Huang, Yu Chen, Yong-Zhou Hu, Yi-Feng Han, Feng Han, Xin Li
Formaldehyde (FA) is endogenously produced in live systems and has been implicated in a diverse array of pathophysiological processes. To disentangle the detailed molecular mechanisms of FA biology, a reliable method for monitoring FA changes in live cells would be indispensable. Although there have been several fluorescent probes reported to detect FA, most are limited by the slow detection kinetics and the intrinsic disadvantage of detecting FA in an irreversible manner which may disturb endogenous FA homeostasis...
2017: Theranostics
https://www.readbyqxmd.com/read/28740517/dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#9
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
BACKGROUND: The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. METHODS: This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28740441/detection-of-ros1-rearrangement-in-non-small-cell-lung-cancer-current-and-future-perspectives
#10
REVIEW
Giulio Rossi, Genny Jocollé, Antonia Conti, Marcello Tiseo, Federica Zito Marino, Giovanni Donati, Renato Franco, Francesca Bono, Francesca Barbisan, Francesco Facchinetti
ROS1 rearrangement characterizes a small subset (1%-2%) of non-small cell lung cancer and is associated with slight/never smoking patients and adenocarcinoma histology. Identification of ROS1 rearrangement is mandatory to permit targeted therapy with specific inhibitors, demonstrating a significantly better survival when compared with conventional chemotherapy. Detection of ROS1 rearrangement is based on in situ (immunohistochemistry, fluorescence in situ hybridization) and extractive non-in situ assays. While fluorescence in situ hybridization still represents the gold standard in clinical trials, this technique may fail to recognize rearrangements of ROS1 with some gene fusion partner...
2017: Lung Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/28739704/vaginal-and-urine-self-sampling-compared-to-cervical-sampling-for-hpv-testing-with-the-cobas-4800-hpv-test
#11
Katrin Christine Asciutto, Anna J Henningsson, Henrik Borgfeldt, Lotten Darlin, Christer Borgfeldt
BACKGROUND/AIM: To compare human papillomavirus (HPV) DNA detection in self-collected vaginal and urine samples with clinician-taken cervical samples in relation to histology. MATERIALS AND METHODS: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 218 women with the Cobas 4800 HPV test (Roche Molecular Diagnostics). RESULTS: The sensitivity for detection of HPV in the vaginal self-sampling test was 96.4% and in urine was 83...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739378/succinate-dehydrogenase-sdh-deficiency-carney-triad-and-the-epigenome
#12
REVIEW
Nikolaos Settas, Fabio R Faucz, Constantine A Stratakis
In this report, we review the relationship between succinate dehydrogenase (SDH) deficiency and the epigenome, especially with regards to two clinical conditions. Carney triad (CT) is a very rare disease with synchronous or metachronous occurrence of at least three different tumor entities; gastric gastrointestinal stromal tumor (GIST), paraganglioma (PGL), and pulmonary chondroma. This condition affects mostly females and it is never inherited. Another disease that shares two of the tumor components of CT, namely GIST and PGL is the Carney-Stratakis syndrome (CSS) or dyad...
July 21, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28739302/comparison-of-the-analytical-and-clinical-performance-of-five-tests-for-the-detection-of-human-papillomavirus-genital-infection
#13
M Del Pino, I Alonso, A Trujillo, S Bernal, D Geraets, N Guimerà, A Torne, J Ordi
HPV-based screening provides greater protection against cervical cancer (CC) than cytology-based strategies. Currently, several molecular diagnostic assays for the detection of human papillomavirus (HPV) are available. In this study, we analyzed 5 different HPV testing and genotyping techniques (Hybrid Capture 2 [HC2; Qiagen, Hilden, Germany], AnyplexTMII HPV28 [Anyplex; Seegene, Seoul, Korea], Linear Array [Roche, Branchburg, NJ, USA], GP5+/6+ PCR-EIA-RH [Labo Bio-medical Products, Rijswijk, The Netherlands] and CLART2 [Genomica, Madrid, Spain]) in 295 women referred to the hospital Colposcopy Clinic from 2007 to 2008 due to positive HPV test results or an abnormal Pap test...
July 21, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28738838/improved-detection-of-genus-specific-alphavirus-using-a-generic-taqman%C3%A2-assay
#14
Claude Giry, Bénédicte Roquebert, Ghislaine Li-Pat-Yuen, Philippe Gasque, Marie-Christine Jaffar-Bandjee
BACKGROUND: Alphaviruses are arthropod borne RNA viruses of medical importance. Geographical expansion of mosquitoes of the Aedes genus in the past decades has been associated with major Alphavirus-associated outbreaks. Climate changes and intensification of air travels have favored vector expansion and virus dissemination in new territories leading to virus emergence not only in tropical areas but also in temperate regions. The detection of emergence is based upon surveillance networks with epidemiological and laboratory investigation...
July 24, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28737890/a-difluoroboron-%C3%AE-diketonate-probe-shows-turn-on-near-infrared-fluorescence-specific-for-tau-fibrils
#15
Kwang-Su Park, Mi Kyoung Kim, Yujin Seo, Taewoong Ha, Kyeongha Yoo, Seung Jae Hyeon, Yu Jin Hwang, Junghee Lee, Hoon Ryu, Hyunah Choo, Youhoon Chong
Tau aggregation in neuronal cells has recently received significant attention as a robust predictor of the progression of Alzheimer's disease (AD) because of its proven correlation with the degree of cognitive impairment in AD patients. Accordingly, non-invasive imaging of tau aggregates has been highlighted as a promising diagnostic tool for AD. We have previously identified a tau-specific "turn-on" near-infrared fluorescent (NIRF) probe (1) and, in this study, structural modification was performed to optimize its physicochemical as well as fluorescence properties...
July 24, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28737550/intestinal-cestodes
#16
Camille Webb, Miguel M Cabada
PURPOSE OF REVIEW: Cestodes infections in humans are among the most prevalent parasitosis worldwide. Although tapeworm infection is often asymptomatic, they can be associated with a range of symptoms. The landscape of cestode infections is changing with rapid diagnosis techniques and advanced molecular diagnosis aiding in identification of species specific epidemiology. RECENT FINDINGS: Traditional descriptions of species by location have been challenged with molecular diagnostic techniques, which show variation in distribution of species, thought to be because of globalization and importation of disease...
July 22, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28736873/standardised-molecular-diagnostic-tool-for-the-identification-of-cryptic-species-within-the-bemisia-tabaci-complex
#17
S Elfekih, W T Tay, K Gordon, L Court, P De Barro
BACKGROUND: The whitefly Bemisia tabaci complex harbours over 40 cryptic species that have been placed in 11 phylogenetically distinct clades based on the molecular characterisation of partial mitochondrial DNA COI (mtCOI) gene region. Four cryptic species are currently within the invasive clade i.e. 'MED', 'MEAM1', 'MEAM2' and IO'. Correct identification of these species is a critical step towards implementing reliable measures for plant biosecurity and border protection, however no standardised B...
July 23, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28736855/-lnc-ing-wnt-in-female-reproductive-cancers-therapeutic-potential-of-long-non-coding-rnas-in-wnt-signaling
#18
REVIEW
Mei S Ong, Wanpei Cai, Yi Yuan, Hin C Leong, Tuan Z Tan, Asad Mohammad, Ming L You, Frank Arfuso, Boon C Goh, Sudha Warrier, Gautam Sethi, Nicholas S Tolwinski, Peter E Lobie, Celestial T Yap, Shing C Hooi, Ruby Y Huang, Alan P Kumar
Recent discoveries in the non-coding genome have challenged the original central dogma of molecular biology; non-coding RNAs and related processes have been found to be important in regulating gene expression. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are amongst some that have gained attention recently in human diseases, including cancer, with the involvement of many more ncRNAs waiting to be discovered. Non-coding RNAs (ncRNAs) are a group of ribonucleic acids transcribed from regions of the human genome, which do not become translated into proteins, despite having essential roles in cellular physiology...
July 24, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28736577/optimization-of-proximity-ligation-assay-pla-for-detection-of-protein-interactions-and-fusion-proteins-in-non-adherent-cells-application-to-pre-b-lymphocytes
#19
Lydie Debaize, Hélène Jakobczyk, Anne-Gaëlle Rio, Virginie Gandemer, Marie-Bérengère Troadec
BACKGROUND: Genetic abnormalities, including chromosomal translocations, are described for many hematological malignancies. From the clinical perspective, detection of chromosomal abnormalities is relevant not only for diagnostic and treatment purposes but also for prognostic risk assessment. From the translational research perspective, the identification of fusion proteins and protein interactions has allowed crucial breakthroughs in understanding the pathogenesis of malignancies and consequently major achievements in targeted therapy...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/28736571/evaluation-of-quality-assessment-protocols-for-high-throughput-genome-resequencing-data
#20
REVIEW
Matteo Chiara, Giulio Pavesi
Large-scale initiatives aiming to recover the complete sequence of thousands of human genomes are currently being undertaken worldwide, concurring to the generation of a comprehensive catalog of human genetic variation. The ultimate and most ambitious goal of human population scale genomics is the characterization of the so-called human "variome," through the identification of causal mutations or haplotypes. Several research institutions worldwide currently use genotyping assays based on Next-Generation Sequencing (NGS) for diagnostics and clinical screenings, and the widespread application of such technologies promises major revolutions in medical science...
2017: Frontiers in Genetics
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