Read by QxMD icon Read


Bice S Martincigh, Moses A Ollengo
The photostabilizing ability of grape seed extract on three common sunscreen absorbers: 2-ethylhexyl-p-methoxcinnamate (EHMC), benzophenone-3 (BP3) and tert-butylmethoxy dibenzoylmethane (BMDBM), was investigated. Samples were exposed to simulated solar radiation and monitored by spectrophotometric and chromatographic methods. The chemical composition of the grape seed extract was determined by GC-MS and HPLC-MS and the major secondary metabolites were found to be epicatechin and catechin. Exposure of the extract to UV radiation increased the UV absorption capacity of the extract...
October 19, 2016: Photochemistry and Photobiology
Ying Chen, Chao He, Jing-Jun Cheng, Wen-Yao Huang, Sheng-Wen Shao, Ya-Ping Jiang, Ling-Feng Dai, Jia-Fa Liu, Yi Song
This study aimed to conduct measurement uncertainty assessment of a new method for determination of Sudan colorants (Sudan I, II, III and IV) in food by high performance liquid chromatography (HPLC). Samples were extracted with organic solvents (hexane, 20% acetone) and first purified by magnesium trisilicate (2MgO·3SiO2). The Sudan colorants (Sudan I-IV) were also initially separated on C8 by gradient elution using acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases and detected with diode-array detector (DAD)...
October 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
Carmen Jiménez-Castells, Jorick Vanbeselaere, Sonja Kohlhuber, Bärbel Ruttkowski, Anja Joachim, Katharina Paschinger
BACKGROUND: The porcine nodule worm Oesophagostomum dentatum is a strongylid class V nematode rather closely related to the model organism Caenorhabditis elegans. However, in contrast to the non-parasitic C. elegans, the parasitic O. dentatum is an obligate sexual organism, which makes both a gender and developmental glycomic comparison possible. METHODS: Different enzymatic and chemical methods were used to release N-glycans from male and female O. dentatum as well as from L3 and L4 larvae...
October 14, 2016: Biochimica et Biophysica Acta
Zhihui Liu, Jianxiu Zhai, Na Han, Jun Yin
ETHNOPHARMACOLOGICAL RELEVANCE: Astilboides tabularis has a long history of usage as the functional food with the effect of adjunctive therapy of diabetic in northeast of China. The present study was undertaken to assess anti-diabetic activity of the aqueous extract of leaves of A. tabularis and provided experimental evidence for the clinical usage of A. tabularis in the treatment of diabetes mellitus. MATERIALS AND METHODS: The aqueous extracts of leaves of A. tabularis (EAT) were orally administered at 300 and 600mg/kg dose to epinephrine-induced and alloxan-induced diabetes mice...
October 14, 2016: Journal of Ethnopharmacology
Fadi Aldeek, Walter Hammack, Mark R Crosswhite, Matthew Standland, Daniele Canzani, Ghislain Gerard, Jo-Marie Cook
Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treat this disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and 20 ng/g) were tested in triplicate...
October 15, 2016: Journal of AOAC International
Seydou Alassane, Christine Binquet, Louis Arnould, Olivier Fleck, Niyazi Acar, Cécile Delcourt, Lionel Bretillon, Alain M Bron, Catherine Creuzot-Garcher
Purpose: We describe the spatial distribution and cross-sectional associations with physical and lifestyle attributes. Methods: The participants of the Montrachet (Maculopathy Optic Nerve, nuTRition neurovAsCular and HEarT) study underwent an exhaustive ophthalmologic examination, including optical coherence tomography, to measure retinal thickness. The spatial distribution of macular pigment optical density (MPOD) defined as a ring-like, intermediate or no-ring pattern, was assessed by means of the two-wavelength autofluorescence technique...
August 1, 2016: Investigative Ophthalmology & Visual Science
Michael A Bergen, Han Na Park, Ranjay Chakraborty, Erica G Landis, Curran Sidhu, Li He, P Michael Iuvone, Machelle T Pardue
Purpose: The neuromodulator dopamine (DA) has been implicated in the prevention of excessive ocular elongation and myopia in various animal models. This study used retina-specific DA knockout mice to investigate the role of retinal DA in refractive development and susceptibility to experimental myopia. Methods: Measurements of refractive error, corneal curvature, and ocular biometrics were obtained as a function of age for both untreated and form-deprived (FD) groups of retina-specific tyrosine hydroxylase knockout (rTHKO) and control (Ctrl) mice...
August 1, 2016: Investigative Ophthalmology & Visual Science
Yves Claude Guillaume, Lydie Lethier, Claire André
TRAIL is a member of the TNF family of cytokines which induces apoptosis of cancer cells via its binding to its cognate receptors, DR5 a high affinity site and DR4 a site of low affinity. Our working group has recently demonstrated that nanovectorization of TRAIL with single wall carbon nanotubes (abbreviated NPT) enhanced TRAIL affinity to the high affinity site DR5 and increased pro apoptotic potential in different human tumor cell lines. In this paper, the DR4 low affinity site was immobilized on a chromatographic support and the effect of temperature on a wide temperature range 1°C-50°C was studied to calculate the thermodynamic parameters of the binding of TRAIL and NPT to DR4 and DR5 receptors...
October 12, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Artem D Rogachev, Olga I Yarovaya, Sergey V Ankov, Mikhail V Khvostov, Tatyana G Tolstikova, Andrey G Pokrovsky, Nariman F Salakhutdinov
A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC-MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20μL of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue...
October 11, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yuli Hu, Shoujun Fan, Xiaobing Liao, Chao Chen, Liang Su, Xuegang Li
Berberine (BBR) is a bioactive plant ingredient derived from the roots and bark of Berberis aristata and Coptis chinensis and has a wide variety of pharmacological effects. 8-cetylberberine (8-BBR-C16) is the berberine (BBR) derivative reconstructed from adding octadecyl at C-8 of BBR to enhance its activity. This study presents a reliable method for the determination of BBR and 8-BBR-C16 in rat plasma, urine and feces. BBR and 8-BBR-C16 were determined by HPLC-UV after liquid-liquid extraction for plasma samples, and solid-phase extraction for urinary and fecal samples...
October 14, 2016: Journal of Pharmaceutical and Biomedical Analysis
Shradha Subedi, Fanrong Kong, Peter Jelfs, Timothy J Gray, Meng Xiao, Vitali Sintchenko, Sharon C-A Chen
Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capillary gel electrophoresis (SCGE). Fourteen American Type Culture Collection (ATCC) strains and 103 clinical/environmental isolates (total n = 24 species) of SG-NTM were included...
2016: PloS One
Delphine Vincent, Aaron Elkins, Mark R Condina, Vilnis Ezernieks, Simone Rochfort
Cow's milk is an important source of proteins in human nutrition. On average, cow's milk contains 3.5% protein. The most abundant proteins in bovine milk are caseins and some of the whey proteins, namely beta-lactoglobulin, alpha-lactalbumin, and serum albumin. A number of allelic variants and post-translationally modified forms of these proteins have been identified. Their occurrence varies with breed, individuality, stage of lactation, and health and nutritional status of the animal. It is therefore essential to have reliable methods of detection and quantitation of these proteins...
2016: PloS One
Said A Hassan, Eman S Elzanfaly, Salem Badr A El-Zeany, Maissa Y Salem
Two methods were developed for separation and quantitation of amlodipine (AML) and atorvastatin (ATV) in the presence of their acidic degradation products. The first method was a simple isocratic RP-HPLC method while the second was capillary electrophoresis (CE). Degradation products were obtained by acidic hydrolysis of the two drugs and their structures were elucidated for the first time by IR and MS spectra. Degradation products did not interfere with the determination of either drug and the assays were therefore stability-indicating...
December 1, 2016: Acta Pharmaceutica
Nayan Patel, Paula Pera, Penny Joshi, Mykhaylo Dukh, Walter A Tabaczynski, Kevin E Siters, Mark Kryman, Ravindra R Cheruku, Farukh Durrani, Joseph R Missert, Ramona Watson, Tymish Y Ohulchanskyy, Erin Tracy, Heinz Baumann, Ravindra K Pandey
We report herein, the synthesis and biological efficacy of near-infrared (NIR), bacteriochlorin analogs: 3-(1'-butyloxy)ethyl-3-deacetyl-bacteriopurpurin-18-N-butylimide methyl ester (3) and the corresponding carboxylic acid 10. In in vitro assays, compared to its methyl ester analog 3, the corresponding carboxylic acid derivative 10 showed higher photosensitizing efficacy. However, due to drastically different pharmacokinetics in vivo, the PS 3 (HPLC purity > 99%) showed higher tumor uptake and long-term tumor cure than 10 (HPLC purity > 96...
October 17, 2016: Journal of Medicinal Chemistry
Ning Xu, Guofu Zhou, Xiaojuan Li, Heng Lu, Fanyun Meng, Huaqiang Zhai
A reliable and comprehensive method for identifying the origin and assessing the quality of Epimedium has been developed. The method is based on analysis of HPLC fingerprints, combined with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and multi-ingredients quantitative analysis. Nineteen batches of Epimedium, collected from different areas in the western regions of China, were used to establish the fingerprints and 18 peaks were selected for the analysis. Similarity analysis, HCA and PCA all classified the 19 areas into three groups...
October 17, 2016: Biomedical Chromatography: BMC
Carola Schultz, Sven Vedder, Martin Winter, Sascha Nowak
The development of a novel high performance liquid chromatography (HPLC) method hyphenated to an ion-trap time-of-flight mass spectrometer (IT-TOF-MS) for the separation and identification of constituents from common organic carbonate solvent-based electrolyte systems in lithium ion batteries (LIBs) is presented in this work. The method development was developed for the qualitative structural elucidation of electrolyte main constituents with a special focus on the aging products of these components. The determination of their limits of detection was performed as well...
October 17, 2016: Analytical Chemistry
Huawei Zhang, Phillip Crews, Karen Tenney, Frederick A Valeriote
BACKGROUND: A growing evidence indicates that marine sponge Phyllospongia sp. is one of rich sources of 20, 24-bishomoscalane sesterterpenes with potent biological activities. In order to search more bioactive 20, 24-bishomoscalane sesterterpenes for new drug discovery, chemical investigation was carried out on an Indonesian marine sponge P. papyrecea. METHODS: Bioassay-guided fractionation was carried out on its dichloromethane extract. And nine compounds were purified and isolated using HPLC...
October 7, 2016: Medicinal Chemistry
Ehsan Mottaghianpour, Firouzeh Nazari, Mohammad Reza Mehrasbi, Mir-Jamal Hosseini
BACKGROUND: Aflatoxin B1 (AFB1 ), as a toxic fungal metabolite, can be found in baby foods, which can lead to serious complications for children's health. In this study, 48 commercial baby foods available in the Iranian market were investigated for the presence of AFB1 , using a high performance liquid chromatography (HPLC) system equipped with post-column photochemical derivatization and a fluorescence detector. RESULTS: In this study, 33 out of 48 samples (68...
October 16, 2016: Journal of the Science of Food and Agriculture
Julian A Harrison, J Andrew Aquilina
The compositions of paradoxin and taipoxin (PDx and TPx, respectively) were investigated using size exclusion chromatography (SEC) and nano-electrospray ionization mass spectrometry (nano-ESI-MS). The elution profiles from size exclusion chromatography of the venoms from Oxyuranus microlepidotus and Oxyuranus scutellatus were similar. Fractions corresponding to the trimeric toxins were treated with guanidinium hydrochloride and the individual subunits were separated by HPLC. In this report we present the size exclusion chromatography profiles for these toxins, and the nano-ESI mass spectra of the subunits after separation by HPLC: the first such comparative study of these toxins at the protein level...
December 2016: Data in Brief
Hubert Antolak, Agata Czyzowska, Dorota Kregiel
The aim of the study was to evaluate the activity of high-polyphenolic black currant (Ribes nigrum L.) and bilberry (Vaccinium myrtillus L.) juices against bacterial strains Asaia lannensis and Asaia bogorensis isolated as spoilage of commercial soft drinks. The composition of fruit juices was evaluated using chromatographic techniques HPLC and LC-MS. The adhesion to glass, polystyrene, and polyethylene terephthalate in two different culture media was evaluated by luminometry and the plate count method. The major anthocyanins in the V...
2016: BioMed Research International
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"