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https://www.readbyqxmd.com/read/28743077/characterization-and-quantitative-analysis-of-phenolic-derivatives-in-longxuetongluo-capsule-by-hplc-dad-it-tof-ms
#1
Jing Sun, Yuelin Song, Hui Sun, Wenjing Liu, Yunfeng Zhang, Jiao Zheng, Qian Zhang, Yunfang Zhao, Wei Xiao, Pengfei Tu, Jun Li
Longxuetongluo Capsule (LTC), which is derived from the total phenolic extract of Chinese dragon's blood, has been proved to be safe as well as effective towards ischemic stroke. However, the effective material basis remains unclear. The present study thereby focused on the clarification of the qualitative and quantitative properties for the phenolic derivatives in LTC. Regarding homolog-focused chemical profiling, the mass fragmentation patterns of the primary subtypes of phenolic compounds such as homoisoflavanones, flavanes, chalcones, and flavonoid oligomers were summarized by assaying authentic references with hybrid ion trap time-of-flight mass spectrometry, and the chemical structures of 124 phenolic compounds, in total, were unambiguously or tentatively annotated in LTC by matching the accurate mass spectral profiles with the proposed mass cracking rules and those reference substances...
July 14, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28741753/phytochemical-and-bioactive-potential-of-in-vivo-and-in-vitro-grown-plants-of-centaurea-ragusina-l-detection-of-dna-rna-active-compounds-in-plant-extracts-via-thermal-denaturation-and-circular-dichroism
#2
Valerija Vujčić, Sandra Radić Brkanac, Ivana Radojčić Redovniković, Siniša Ivanković, Ranko Stojković, Irena Žilić, Marijana Radić Stojković
INTRODUCTION: The phytochemical composition and biological activity of non-volatile components of Centaurea ragusina L. has not been studied previously. OBJECTIVES: Our aim was to evaluate the phytochemical and bioactive potential (including interactions with polynucleotides) of C. ragusina L. depending on the origin of plant material (in vivo - leaves from natural habitats, ex vitro - leaves from plants acclimated from culture media, in vitro - leaves and calli from plants grown in culture media) and polarity of solvents used in extract preparation (80 and 96% ethanol and water combinations or single solvents)...
July 25, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28741668/pharmacokinetics-of-deflazacort-in-rabbits-after-intravenous-and-oral-administration-and-its-interaction-with-erythromycin
#3
A I Escudero, P Marín, C M Cárceles, E Escudero
The pharmacokinetic of deflazacort after intravenous and oral administration and the effect of erythromycin on the disposition of deflazacort in rabbits were investigated. A parallel study was carried out in twelve rabbits. The plasma concentration-time profiles of deflazacort were determined after intravenous and oral administration of single dosages of 5 mg/kg in the presence and absence (baseline) of multiple dose erythromycin regimens. Plasma concentrations of 21-desacetyldeflazacort were determined by HPLC...
July 25, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28741107/development-and-optimization-of-a-novel-sample-preparation-method-cored-on-functionalized-nanofibers-mat-solid-phase-extraction-for-the-simultaneous-efficient-extraction-of-illegal-anionic-and-cationic-dyes-in-foods
#4
Feifei Qi, Ningge Jian, Liangliang Qian, Weixin Cao, Qian Xu, Jian Li
A simple and efficient three-step sample preparation method was developed and optimized for the simultaneous analysis of illegal anionic and cationic dyes (acid orange 7, metanil yellow, auramine-O, and chrysoidine) in food samples. A novel solid-phase extraction (SPE) procedure based on nanofibers mat (NFsM) was proposed after solvent extraction and freeze-salting out purification. The preferred SPE sorbent was selected from five functionalized NFsMs by orthogonal experimental design, and the optimization of SPE parameters was achieved through response surface methodology (RSM) based on the Box-Behnken design (BBD)...
July 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28740758/identification-and-characterization-of-streptomyces-flavogriseus-nj-4-as-a-novel-producer-of-actinomycin-d-and-holomycin
#5
Zhaohui Wei, Chao Xu, Juan Wang, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu
This paper is the first public report that Streptomyces flavogriseus can produce both actinomycin D and holomycin. The actinomycete strain NJ-4 isolated from the soil of Nanjing Agricultural University was identified as S. flavogriseus. This S. flavogriseus strain was found for the first time to produce two antimicrobial compounds that were identified as actinomycin D and holomycin. GS medium, CS medium and GSS medium were used for the production experiments. All three media supported the production of actinomycin D, while holomycin was detected only in GS medium and was undetectable by HPLC in the CS and GSS media...
2017: PeerJ
https://www.readbyqxmd.com/read/28739601/buddleja-thyrsoides-lam-crude-extract-presents-antinociceptive-effect-on-an-arthritic-pain-model-in-mice
#6
Maria Fernanda Fialho, Indiara Brusco, Evelyne Silva Brum, Mariana Piana, Aline Augusti Boligon, Gabriela Trevisan, Sara Marchesan Oliveira
The arthritis is a chronic inflammatory disease which reduces the life quality of affected individuals. Therapeutic tools used to treat inflammatory pain are associated with several undesirable effects.  Buddleja thyrsoides Lam, known as 'Barbasco' or 'Cambara', is popularly used in several disorders and possess antirheumatic, anti-inflammatory and analgesic activities. Here, we investigated the antinociceptive and anti-inflammatory effect of the oral and topical treatment from the B.thyrsoides crude extract in acute pain models and an arthritic pain model induced by complete Freund's adjuvant paw injection in male mice (25-30g)...
July 23, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28739273/analysis-of-packing-microstructure-and-wall-effects-in-a-narrow-bore-ultrahigh-pressure-liquid-chromatography-column-using-focused-ion-beam-scanning-electron-microscopy
#7
Arved E Reising, Sabine Schlabach, Vasili Baranau, Daniela Stoeckel, Ulrich Tallarek
Column wall effects are well recognized as major limiting factor in achieving high separation efficiency in HPLC. This is especially important for modern analytical columns packed with small particles, where wall effects dominate the band broadening. Detailed knowledge about the packing microstructure of packed analytical columns has so far not been acquired. Here, we present the first three-dimensional reconstruction protocol for these columns utilizing focused ion-beam scanning electron microscopy (FIB-SEM) on a commercial 2...
July 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28739272/an-on-line-analytical-approach-for-detecting-haptens-in-shuang-huang-lian-powder-injection
#8
Hong Wang, Xiaotian Zhang, Daidong Wang, Hongyang Sun, Yuexiang Lan, Haixiu Jiang, Shizhong Chen, Zongtao Lin
Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine injection (TCMI) frequently used in the clinical treatment of faucitis, bronchitis, and other viral and bacterial infections of upper respiratory tract. However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, have been a serious problem of its clinical safety. This problem has not been solved due to short of methods for detecting haptens in complex TCMIs. In this study, an on-line high-performance liquid chromatography-diode-array detector-mass spectrometry combined with bovine serum albumin-fluorescence detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, validated and applied for identification of haptens in SHLPI...
July 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28739201/neonatal-detection-of-aicardi-gouti%C3%A3-res-syndrome-by-increased-c26-0-lysophosphatidylcholine-and-interferon-signature-on-newborn-screening-blood-spots
#9
Thais Armangue, Joseph J Orsini, Asako Takanohashi, Francesco Gavazzi, Alex Conant, Nicole Ulrick, Mark A Morrissey, Norah Nahhas, Guy Helman, Heather Gordish-Dressman, Simona Orcesi, Davide Tonduti, Chloe Stutterd, Keith van Haren, Camilo Toro, Alejandro D Iglesias, Marjo S van der Knaap, Raphaela Goldbach Mansky, Anne B Moser, Richard O Jones, Adeline Vanderver
BACKGROUND: Aicardi Goutières Syndrome (AGS) is a heritable interferonopathy associated with systemic autoinflammation causing interferon (IFN) elevation, central nervous system calcifications, leukodystrophy and severe neurologic sequelae. An infant with TREX1 mutations was recently found to have abnormal C26:0 lysophosphatidylcholine (C26:0 Lyso-PC) in a newborn screening platform for X-linked adrenoleukodystrophy, prompting analysis of this analyte in retrospectively collected samples from individuals affected by AGS...
July 20, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28738692/flavonoid-profile-antioxidant-and-antiglycation-properties-of-retama-sphaerocarpa-fruits-extracts
#10
S Boussahel, F Cacciola, S Dahamna, L Mondello, A Saija, F Cimino, A Speciale, M Cristani
Retama sphaerocarpa occurs in the Mediterranean area of North-east Africa and in the Iberian Peninsula, and grows on a variety of soil types and climatic conditions. Used in Algerian folk medicine, it is a valuable species for revegetation and soil restoration. The aim of this study is to evaluate flavonoid composition and antioxidant and antiglycation properties of methanolic and aqueous extracts from R. sphaerocarpa fruits. HPLC-PDA/ESI-MS was used to identify/quantify flavonoid content. Antioxidant activity was evaluated by Folin-Ciocalteu, ORAC, FRAP, TEAC, and DPPH assays, and antiglycation capability by glucose/fructose-BSA assay...
July 24, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28738649/well-defined-magnetic-surface-imprinted-nanoparticles-for-selective-enrichment-of-2-4-dichlorophenoxyacetic-acid-in-real-samples
#11
Le Sheng, Yulong Jin, Yonghuan He, Yanyan Huang, Liushui Yan, Rui Zhao
Superparamagnetic core-shell molecularly imprinted polymer nanoparticles (MIPs) were prepared via surface initiated reversible-addition fragmentation chain transfer (si-RAFT) polymerization for the selective recognition of 2,4-dichlorophenoxyacetic acid (2,4-D) in real samples. The construction of uniform core-shell structure with a 50nm MIP layer was successfully accomplished, which favored mass transfer and resulted in fast recognition kinetics. The static equilibrium experiments revealed the satisfied adsorption capacity and imprinting efficiency of Fe3O4@MIP...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738646/synthesis-characterization-and-application-of-a-novel-multifunctional-stationary-phase-for-hydrophilic-interaction-reversed-phase-mixed-mode-chromatography
#12
Hayriye Aral, K Serdar Çelik, Ramazan Altındağ, Tarık Aral
A novel multifunctional stationary phase based on silica gel was synthesised starting from L- isoleucine and 4-phenylbutylamine and evaluated as a hydrophilic interaction/reversed-phase mixed-mode stationary phase for high-performance liquid chromatography (HPLC). The prepared stationary phase was characterized by elemental analysis, infrared spectroscopy (IR), scanning electron microscopy (SEM), Brunauer, Emmett and Teller (BET) and solid-state (13)C nuclear magnetic resonance (NMR). The mechanisms involved in the chromatographic separation are multi-interaction, including hydrophobic, π-π, hydrogen-bonding, dipole-dipole and ion-dipole interactions...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738573/stable-isotope-ratio-analysis-for-authentication-of-red-yeast-rice
#13
Matteo Perini, Gianfranco Carbone, Federica Camin
Red yeast rice (RYR) is a dietary supplement obtained from rice fermented with the mould Monascus purpureus. It contains Monacolin K which is a hypocholesterolemic statin used to prevent cardiovascular diseases. The homologous prescription biosynthetic statin, lovastatin, is not chemically distinguishable from monacolin K. In this work we investigated whether δ(13)C and δ(2)H can distinguish monacolin K from lovastatin and can detect the presence of lovastatin in RYR. 18 samples of red yeast rice powder and 18 samples of lovastatin were collected...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738566/preparation-and-evaluation-of-a-novel-n-benzyl-phenethylamino-%C3%AE-cyclodextrin-bonded-chiral-stationary-phase-for-hplc
#14
Liang Li, Biaoping Cheng, Rendan Zhou, Zhigang Cao, Chun Zeng, Laisheng Li
A novel N-benzyl-phenethylamino-β-cyclodextrin-bonded ordered mesoporous SBA-15 chiral stationary phase (BZCDP) for high-performance liquid chromatography (HPLC) was prepared. The structure and morphology of the ligand and the stationary phase were characterized by mass spectrometry, elemental analysis, infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis. The enantiomers of chiral compounds including nine common β-adrenergic blocker drugs (β-blockers), eight dansyl amino acids (DNS-AA) and six flavanones were successfully separated by polar organic solvent and reversed-phase chromatography, respectively...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738543/phenolic-compounds-of-triplaris-gardneriana-can-protect-cells-against-oxidative-stress-and-restore-oxidative-balance
#15
Thiago Silva de Almeida, José Joaquim Lopes Neto, Nathanna Mateus de Sousa, Igor Parra Pessoa, Leonardo Rogério Vieira, Jackeline Lima de Medeiros, Aline Augusti Boligon, Astrid R M Hamers, Davi Felipe Farias, Ad Peijnenburg, Ana Fontenele Urano Carvalho
This work aimed to add value to an underexploited plant species from Brazil, Triplaris gardneriana. To that, the phenolic compounds profile of its seed ethanolic extract and fractions was examined by HPLC and the antioxidant capacity assessed using chemical assays as well as in vitro cell imaging. Twelve compounds were quantified and classified as either phenolic acids or flavonoids. The fractionation process did not generate fractions with different compositions except for chloroformic fraction, which showed only 6 out of 12 standard compounds used...
July 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28737900/accumulation-of-charantin-and-expression-of-triterpenoid-biosynthesis-genes-in-bitter-melon-momordica-charantia
#16
Do Manh Cuong, Jin Jeon, Abubaker M A Morgan, Changsoo Kim, Jae Kwang Kim, Sook Young Lee, Sang Un Park
Charantin, a natural cucurbitane type triterpenoid, has been reported to have beneficial pharmacological functions such as anticancer, antidiabetic, and antibacterial activities. However, accumulation of charantin in bitter melon has been little studied. Here, we performed a transcriptome analysis to identify genes involved in the triterpenoid biosynthesis pathway in bitter melon seedlings. Total of 88,703 transcripts with an average length of 898 bp were identified in bitter melon seedlings. Based on a functional annotation, we identified 15 candidate genes encoding enzymes related to triterpenoid biosynthesis and analyzed their expression in different organs of mature plants...
July 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28737713/quality-assessment-of-gentiana-rigescens-from-different-geographical-origins-using-ft-ir-spectroscopy-combined-with-hplc
#17
Zhe Wu, Yanli Zhao, Ji Zhang, Yuanzhong Wang
Gentiana rigescens is a precious herbal medicine in China because of its liver-protective and choleretic effects. A method for the qualitative identification and quantitative evaluation of G. rigescens from Yunnan Province, China, has been developed employing Fourier transform infrared (FT-IR) spectroscopy and high performance liquid chromatography (HPLC) with the aid of chemometrics such as partial least squares discriminant analysis (PLS-DA) and support vector machines (SVM) regression. Our results indicated that PLS-DA model could efficiently discriminate G...
July 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737700/a-novel-brominated-alkaloid-securidine-a-isolated-from-the-marine-bryozoan-securiflustra-securifrons
#18
Priyanka Michael, Kine Ø Hansen, Johan Isaksson, Jeanette H Andersen, Espen Hansen
A novel brominated alkaloid, Securidine A, was isolated from the cold water marine bryozoan Securiflustra securifrons. Securidine A was isolated using semi-preparative HPLC, and the structure was elucidated by spectroscopic methods. The isolated Securidine A was tested for cytotoxic, antibacterial, and anti-diabetic activities as well as for its potential for inhibition of biofilm formation. No significant biological activity was observed in the applied bioassays, thus expanded bioactivity profiling is required, in order to reveal any potential applications for Securidine A...
July 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737445/preclinical-pharmacokinetics-and-biodistribution-studies-of-asenapine-maleate-using-novel-and-sensitive-rp-hplc-method
#19
Renuka S Managuli, Karthik Gourishetti, Rekha R Shenoy, Kunnatur B Koteshwara, Meka Sreenivasa Reddy, Srinivas Mutalik
AIM: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. EXPERIMENTAL: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. RESULTS: ASPM was extracted from plasma and tissue matrix by liquid-liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3...
July 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28737428/chromatographic-determination-of-zonisamide-topiramate-and-sulpiride-in-plasma-by-a-fluorescent-turn-on-chemosensor
#20
Fawzia A Ibrahim, Amira F El-Yazbi, Mira M Wagih, Magda A Barary
AIM: Antiepileptics (AEDs) and antipsychotics are often coprescribed. Interactions between these drugs may affect both efficacy and toxicity. Therefore, drug monitoring is necessary for appropriate dosage adjustments. MATERIALS & METHODS: Specific 'turn-on' chemosensor, 4-chloro-7-nitrobenzofurazan is used for selective and sensitive determination of two AEDs: zonisamide (ZON) and topiramate (TOP) with the antipsychotic sulpiride (SUL) in epileptic patients' plasma followed by reversed-phase-HPLC separation without any interference...
July 24, 2017: Bioanalysis
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