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https://www.readbyqxmd.com/read/28445738/ochratoxin-a-and-its-metabolites-in-urines-of-german-adults-an-assessment-of-variables-in-biomarker-analysis
#1
Nurshad Ali, Katherine Muñoz, Gisela H Degen
Ochratoxin A (OTA), a mycotoxin known for its nephrotoxic and carcinogenic properties, is a worldwide occurring contaminant in a variety of food commodities. Biomonitoring (i.e. analysis in biological fluids) can serve to assess human internal exposure from all consumed foods and beverages. We now determined the concentration of OTA and its metabolite ochratoxin alpha (OTα) in plasma and in urine of two male volunteers with different food habits, in order to assess intra-individual temporal fluctuations and inter-individual differences in their biomarker levels...
April 23, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28445422/bioactive-components-from-qingwen-baidu-decoction-against-lps-induced-acute-lung-injury-in-rats
#2
Qi Zhang, Hai-Min Lei, Peng-Long Wang, Zhi-Qiang Ma, Yan Zhang, Jing-Jing Wu, Jing Nie, Su-Juan Chen, Wen-Jie Han, Qing Wang, Dan-Yang Chen, Cheng-Ke Cai, Qiang Li
Qingwen Baidu Decoction (QBD) is an extraordinarily "cold" formula. It was traditionally used to cure epidemic hemorrhagic fever, intestinal typhoid fever, influenza, sepsis and so on. The purpose of this study was to discover relationships between the change of the constituents in different extracts of QBD and the pharmacological effect in a rat model of acute lung injury (ALI) induced by lipopolysaccharide (LPS). The study aimed to discover the changes in constituents of different QBD extracts and the pharmacological effects on acute lung injury (ALI) induced by LPS...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28445409/quantification-of-a-novel-photosensitizer-of-chlorin-e6-c15-monomethyl-ester-in-beagle-dog-plasma-using-hplc-application-to-pharmacokinetic-studies
#3
Xiuxiu Li, Jun Wen, Jingjing Jiang, Xin Zhao, Tingting Zhou, Guorong Fan
Chlorin e6-C15-monomethyl ester (CMME) is a novel photosensitizer, which is synthetized from the degradation products of silkworm excrement. Preclinical studies on the promising photosensitizer CMME are necessary to determine its therapeutic efficacy and druglikeness. A high-performance liquid chromatography with UV detection (HPLC-UV) method was established for the determination of CMME in beagle dog plasma. The sample preparation involved a protein-precipitation method with acetonitrile after the addition of tanshinone IIA as an internal standard (IS)...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444982/ionic-liquid-based-ultrasound-assisted-extraction-of-isoflavones-from-belamcanda-chinensis-and-subsequent-screening-and-isolation-of-potential-%C3%AE-glucosidase-inhibitors-by-ultrafiltration-and-semi-preparative-high-performance-liquid-chromatography
#4
Senlin Li, Sainan Li, Yu Huang, Chunming Liu, Lina Chen, Yuchi Zhang
The separation of a compound of interest from its structurally similar homologues to produce high-purity natural products is a challenging problem. This work proposes a novel method for the separation of iristectorigenin A from its structurally similar homologues by ionic liquid-based ultrasound-assisted extraction and the subsequent screening and isolation of potential α-glucosidase inhibitors via ultrafiltration and semi-preparative high-performance liquid chromatography. Ionic-liquid-based ultrasound-assisted extraction was successfully applied to the extraction of tectorigenin, iristectorigenin A, irigenin, and irisflorentin from Belamcanda chinensis...
April 26, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28444808/fast-and-simple-discriminative-analysis-of-anthocyanins-containing-berries-using-lc-ms-spectral-data
#5
Heejung Yang, Hyun Woo Kim, Yong Soo Kwon, Ho Kyong Kim, Sang Hyun Sung
INTRODUCTION: Anthocyanins are potent antioxidant agents that protect against many degenerative diseases; however, they are unstable because they are vulnerable to external stimuli including temperature, pH and light. This vulnerability hinders the quality control of anthocyanin-containing berries using classical high-performance liquid chromatography (HPLC) analytical methodologies based on UV or MS chromatograms. OBJECTIVE: To develop an alternative approach for the quality assessment and discrimination of anthocyanin-containing berries, we used MS spectral data acquired in a short analytical time rather than UV or MS chromatograms...
April 25, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28444606/species-evolution-and-quality-evaluation-of-four-coptis-herbal-medicinal-materials-in-southwest-china
#6
Hanting Chen, Gang Fan, Yang He
Coptis herbs, which are important herbal medicinal materials, are the dried rhizomes of various plants. In China's herbal market, drying herbs can severely influence morphological markers such as shape, color, and odor, which make consumers difficult to precisely identify the herbs and effectively evaluate the quality. Here, we present the phylogenetic analysis of four Coptis herbal medicinal materials based on internal transcribed spacer sequences. C. chinensis, C. omeiensis, C. deltoidea and C. teeta constituted a monophyletic group...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444354/easy-assessment-of-levofloxacin-and-minocycline-in-relevant-biomimetic-media-by-hplc-uv-analysis
#7
Ana C Matos, Rosana V Pinto, Ana F Bettencourt
Simple, economic and environmental friendly high-performance liquid chromatography methods for levofloxacin and minocycline quantification in biomimetic media were developed and validate including their stability at body temperature, an often neglected evaluation parameter. Both methods are similar only differing in the wavelength setting, i.e., for levofloxacin and minocycline quantification the UV detection was set at 284 and at 273 nm, respectively. The separation of both antibiotics was achieved using a reversed-phase column and a mobile phase consisting of acetonitrile and water (15:85) with 0...
April 24, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28443909/effect-of-the-calcination-temperature-on-the-photocatalytic-efficiency-of-acidic-sol-gel-synthesized-tio2-nanoparticles-in-the-degradation-of-alprazolam
#8
Andreia Romeiro, Diana Freitas, M Emília Azenha, Moisés Canle, Hugh D Burrows
We report a comparative study on the photodegradation of the widely used benzodiazepine psychoactive drug alprazolam (8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine, ALP) using direct photolysis, and titanium dioxide photocatalyzed reaction. Titanium dioxide photocatalysts were prepared as nanoparticles by acidic sol-gel methods, calcined at two different temperatures, and their behavior compared with P25 (Degussa type) TiO2. Efficient photodegradation was observed in the photocatalytic process, with over 90% degradation after 90 minutes under optimized conditions...
April 26, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28443841/automatic-concentration-and-reformulation-of-pet-tracers-via-microfluidic-membrane-distillation
#9
Philip H Chao, Jeffery Collins, Joseph P Argus, Wei-Yu Tseng, Jason T Lee, R Michael van Dam
Short-lived radiolabeled tracers for positron emission tomography (PET) must be rapidly synthesized, purified, and formulated into injectable solution just prior to imaging. Current radiosynthesizers are generally designed for clinical use, and the HPLC purification and SPE formulation processes often result in a final volume that is too large for preclinical and emerging in vitro applications. Conventional technologies and techniques for reducing this volume tend to be slow, resulting in radioactive decay of the product, and often require manual handling of the radioactive materials...
April 26, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28443078/phytochemical-characterization-of-terminalia-catappa-linn-extracts-and-their-antifungal-activities-against-candida-spp
#10
Analucia G Terças, Andrea de Souza Monteiro, Eduardo B Moffa, Julliana R A Dos Santos, Eduardo M de Sousa, Anna R B Pinto, Paola C da Silva Costa, Antonio C R Borges, Luce M B Torres, Allan K D Barros Filho, Elizabeth S Fernandes, Cristina de Andrade Monteiro
Terminalia catappa Linn bark is used to treat dysentery by various populations in Southeast Asian countries, and its leaves have also been used in traditional medicine to treat hepatitis in India and the Philippines. Here, the antifungal actions of crude hydro-alcoholic extract (TcHE) and fractions from T. catappa leaves were assessed via the agar diffusion and microdilution tests on Candida reference strains and clinical isolates from patients with acquired immunodeficiency syndrome (AIDS). Additionally, the potential cytotoxic effects of TcHE were assessed on cultured human peripheral blood mononuclear cells (PBMC)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28442372/long-term-release-and-stability-of-pharmaceutical-proteins-delivered-from-solid-lipid-implants
#11
Moritz Vollrath, Julia Engert, Gerhard Winter
Solid lipid implants (SLIs) prepared by twin-screw (tsc) extrusion represent a promising technology platform for the sustained release of pharmaceutical proteins. In this work, we report on two aspects, long-term release and stability of released protein. First, SLIs were produced by tsc-extrusion containing the low melting triglyceride H12 and the high melting triglyceride Dynasan D118. Two different proteins available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) were incorporated into the lipid matrix: a monoclonal antibody (mAb) from the IgG1 class and the fab-fragment Ranibizumab (Lucentis(®))...
April 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28442116/determination-of-delta-9-tetrahydrocannabinol-content-of-cannabis-seizures-in-egypt
#12
Ahmed M A Souleman, Alaa El-Din M Gaafar, Omar M Abdel-Salam, Shaimaa A ElShebiney
OBJECTIVE: To determine the delta-9-tetrahydrocannabinol (THC) content of cannabis seizures in Egypt. METHODS: Unheated and heated extracts of cannabis seizures were prepared from the dried flowering tops and leaves (marijuana) or from the resin (hashish) and subjected to analysis using high performance liquid chromatography (HPLC). RESULTS: The heated resin extract had the peak of THC in a relative ratio of 31.34%, while extracting the resin directly without heating contained only 18...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28442111/toxicity-assessment-and-screening-of-tetrodotoxin-in-the-oblong-blowfish-takifugu-oblongus-from-the-tamil-nadu-coast-of-bay-of-bengal-india
#13
S M Indumathi, S S Khora
OBJECTIVE: To assess the toxicity of the pufferfish Takifugu oblongus, from Chennai coast, Tamil Nadu, India and to detect the presence of Tetrodotoxin (TTX). METHODS: The toxicity was evaluated by mouse bioassay using Swiss Albino mice which were expressed in mouse units (MU). Gross anatomical features were observed which is followed by histopathology of the dead mice tissues to establish the toxicity. Instrumental analysis for the presence of tetrodotoxin was also performed through GC-MS and HPLC...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28442026/neuromodulatory-effect-of-oestradiol-in-the-metabolism-of-ovarian-progesterone-and-oestradiol-during-dioestrus-ii-participation-of-the-superior-mesenteric-ganglion
#14
Adriana Vega Orozco, Cynthia Bronzi, Sandra Vallcaneras, Zulema Sosa, Marilina Casais
The aims of the present study were to determine: (1) whether oestradiol (E2) in the superior mesenteric ganglion (SMG) modifies the release of ovarian progesterone (P4), androstenedione (A2) and E2, the activity and gene expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-HSD and the expression of P450 aromatase (Cyp19a1) and (2) whether any such modifications are related to changes in ovarian nitric oxide (NO) and noradrenaline (NA) levels during dioestrus II. Using an ex vivo SMG-ovarian nervous plexus-ovary system, ovarian P4 release was measured following the addition E2 plus tamoxifen (Txf) (10-6M) to the ganglion, whereas A2, E2, NA and NO were measured following the addition of E2 alone...
April 26, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28441627/effect-of-in%C3%A2-vitro-drought-stress-on-phenolic-acids-flavonols-saponins-and-antioxidant-activity-in-agave-salmiana
#15
César A Puente-Garza, Cristina Meza-Miranda, Desiree Ochoa-Martínez, Silverio García-Lara
This work evaluated the effect of in vitro drought stress on morphological characteristics, phenolic compounds, flavonols, saponin content, and antioxidant activity in plantlets of Agave salmiana. Drought stress was induced with polyethylene glycol (PEG) at 0, 10, 20 and 30% w/v in Murashige and Skoog solid medium. The determination of specific flavonols and saponins was achieved via HPLC-DAD and HPLC-ELSD, respectively. Compared with the control, plants grown in 30% PEG showed a change in the width of the leaves and a different color, showing less clarity and more darkening (L = 21...
April 10, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28441523/carbohydrates-and-gibberellins-relationship-in-potato-tuberization
#16
Hana Ševčíková, Petra Mašková, Danuše Tarkowská, Tomáš Mašek, Helena Lipavská
Potato represents the third most important crop worldwide and therefore to understand regulations of tuber onset is crucial from both theoretical and practical points of view. Photosynthesis and related carbohydrate status along with phytohormone balance belong to the essential factors in regulation of plant development including storage organ formation. In our work we used potato (Solanum tuberosum) cv. Lada and its spontaneously tuberizing mutant (ST plants) grown in vitro under low carbohydrate availability (non-inductive conditions)...
April 8, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28441364/indole-derivatives-produced-by-the-metagenome-genes-of-the-escherichia-coli-harboring-marine-sponge-discodermia-calyx
#17
Feng-Lou Liu, Xiao-Long Yang
Three indole derivatives, a novel benzoxazine-indole hybrid (1) and two known indole trimers (2, 3), were isolated from the metagenomic library of the marine sponge Discodermia calyx based on functional screening. Their structures were elucidated by extensive spectroscopic analysis and comparison of their NMR data to that of known compounds. The antibacterial assay indicated that only compound 2 displayed significant antibacterial activity against Bacillus cereus, with approximately 20 mm diameter growth inhibition at 10 µg/paper...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28440893/an-hplc-method-for-simultaneous-quantitative-determination-of-seven-secoiridoid-glucosides-separated-from-the-roots-of-ilex-pubescens
#18
Yang Zhang, Huan Xiong, Jiayi Bi, Guiyun Zhao, Yi Yang, Xue Chen, Yuncong Li, Chongyu Zhang, Guogang Zhang
A simple and specific high-performance liquid chromatographic method has been developed and validated to simultaneously determine seven secoiridoid glucosides for the first time. And three of them were separated from the ethanolic extract of the roots of Ilex pubescens for the first time, namely nuezhenide A, ligusides B and oleonuezhenide. In quantitative analysis, all of the calibration curves showed good linear regression (r > 0.999) within the tested ranges, and the mean recoveries of three different concentrations ranged from 97...
April 25, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28440421/allicin-induces-the-upregulation-of-abca1-expression-via-ppar%C3%AE-lxr%C3%AE-signaling-in-thp-1-macrophage-derived-foam-cells
#19
Xiao-Long Lin, Hui-Jun Hu, Yuan-Bo Liu, Xue-Mei Hu, Xiao-Juan Fan, Wei-Wen Zou, Yong-Quan Pan, Wen-Quan Zhou, Min-Wen Peng, Cai-Hong Gu
Allicin is considered anti-atherosclerotic due to its antioxidant and anti-inflammatory effects, which makes it an important drug for the prevention and treatment of atherosclerosis. However, the effects of allicin on foam cells are unclear. Thus, in this study, we examined the effects of allicin on lipid accumulation via peroxisome proliferator-activated receptor γ (PPARγ)/liver X receptor α (LXRα) in THP‑1 macrophage-derived foam cells. THP‑1 cells were exposed to 100 nM phorbol myristate acetate (PMA) for 24 h, and then to oxydized low-density lipoprotein (ox-LDL; 50 mg/ml) to induce foam cell formation...
April 11, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28440419/different-effects-of-%C3%A2-borneol-and-%C3%A2-%C3%A2-borneol-on-the-pharmacokinetics-of-osthole-in-rats-following-oral-administration
#20
Dan-Dan Luo, Xiao-Ying Chen, Zhen-Biao Zhang, Chao-Yue Sun, Yi-Feng Zheng, Yu-Hong Liu, Xiu-Fen Wang, Qi Wang, Janis Ya-Xian Zhan, Zi-Ren Su
Osthole is the primary active component of a number of herbal plants such as the Cnidium monnieri fruit. In traditional Chinese herb medicine, osthole is commonly used in combination with borneol to obtain improved pharmacological effects. The aim of the present study was to investigate the effect of borneol enantiomers on the pharmacokinetics of osthole. An appropriate high‑performance liquid chromatography (HPLC) method was applied to determine the concentrations of osthole in plasma. Following oral administration of osthole alone or combined with borneol in rats, blood samples were collected and analyzed by HPLC...
April 21, 2017: Molecular Medicine Reports
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