keyword
MENU ▼
Read by QxMD icon Read
search

HPLC

keyword
https://www.readbyqxmd.com/read/29777680/assay-for-abscisic-acid-8-hydroxylase-activity-of-cloned-plant-cytochrome-p450-oxidases-in-saccharomyces-cerevisiae
#1
Stella Eggels, Viktoriya Avramova, Chris-Carolin Schön, Brigitte Poppenberger, Wilfried Rozhon
The plant hormone abscisic acid (ABA) regulates many processes, including response to drought, seed dormancy and abscission of leaves and fruits. For maintenance of ABA homeostasis, catabolism of ABA by 8'-hydroxylation and subsequent cyclisation to phaseic acid (PA) is crucial. However, detection of ABA 8'-hydroxylation activity is tedious. We present a simple and rapid method for detection of ABA 8'-hydroxylase activity by cloning cDNAs of interest and expressing the respective protein in yeast. Upon addition of ABA, PA is formed and subsequently quantified in the yeast cell culture supernatant by heart cutting 2D-HPLC or GC-MS...
May 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29777615/tools-for-work-up-and-pre-purification-of-tritium-labelled-small-molecules
#2
Dieter Muri, Martin R Edelmann
This practitioner protocol describes the use and application of different types of solid phase extractions and metal scavengers for small scale reactions, particularly in the context of purifying tritiated molecules from heavily contaminated reaction mixtures. Polymer-bound strong cation exchangers are especially suitable for separating basic compounds from neutral or acidic molecules and have been widely applied in the work-up of Iridium-based hydrogen isotope exchange reactions. Polymer-bound strong anion exchangers can help to separate acidic compounds from their neutral or basic counterparts or to easily convert a TFA or formate salt to its free base after HPLC purification...
May 19, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29777610/phenolic-rich-extracts-of-eurycoma-longifolia-and-cylicodiscus-gabunensis-inhibit-enzymes-responsible-for-the-development-of-erectile-dysfunction-and-are-antioxidants
#3
Ganiyu Oboh, Adeniyi A Adebayo, Ayokunle O Ademosun
BACKGROUND: Herbs have been used from ages to manage male sexual dysfunction. Hence, this study sought to investigate the effects of Eurycoma longifolia (EL) and Cylicodiscus gabunensis (CG) stem bark extracts on some enzymes implicated in erectile dysfunction in vitro. METHODS: The extracts were prepared, and their effects on phosphodiesterase-5 (PDE-5), arginase, and angiotensin-1-converting enzyme (ACE) as well as pro-oxidant-induced lipid peroxidation were assessed...
May 19, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29777444/comparison-of-lawsone-contents-among-lawsonia-inermis-plant-parts-and-neurite-outgrowth-accelerators-from-branches
#4
Yoshimi Oda, Souichi Nakashima, Erina Kondo, Seikou Nakamura, Mamiko Yano, Chisa Kubota, Yusuke Masumoto, Minami Hirao, Yuki Ogawa, Hisashi Matsuda
Lawsonia inermis L. (Lythraceae) is cultivated in many countries, including Japan, China, India and Egypt. Its leaves are well known as hair dye and exhibit antibacterial, anticancer, antifungal and anticonvulsant activities. However, there are few reports on its branches. 2-Hydroxy-1,4-naphthoquinone (lawsone) is a characteristic compound in its leaves. However, it remains to be clarified whether lawsone is present in other plant parts or not. In this study, we measured lawsone contents in the extracts of L...
May 17, 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29777287/decreased-plasma-levels-of-gasotransmitter-hydrogen-sulfide-in-patients-with-schizophrenia-correlation-with-psychopathology-and-cognition
#5
Jian-Wen Xiong, Bo Wei, Yan-Kun Li, Jin-Qiong Zhan, Shu-Zhen Jiang, Hai-Bo Chen, Kun Yan, Bin Yu, Yuan-Jian Yang
OBJECTIVE: Aberrant N-methyl-D-aspartate receptor (NMDAR) function has been implicated in the pathophysiology of schizophrenia. Hydrogen sulfide (H2 S) is an endogenous gasotransmitter that regulates NMDAR function. The current study investigated the relationship between plasma H2 S levels and both psychopathological and cognitive symptoms in schizophrenia. MATERIALS AND METHODS: Forty-one patients with schizophrenia and 40 healthy control subjects were recruited in present study...
May 18, 2018: Psychopharmacology
https://www.readbyqxmd.com/read/29776421/evidence-for-a-hyper-reductive-redox-in-a-sub-set-of-heart-failure-patients
#6
Thiagarajan Sairam, Amit N Patel, Meenu Subrahmanian, Rajendiran Gopalan, Steven M Pogwizd, Sudha Ramalingam, Ramalingam Sankaran, Namakkal Soorapan Rajasekaran
BACKGROUND: Oxidative stress has been linked to heart failure (HF) in humans. Antioxidant-based treatments are often ineffective. Therefore, we hypothesize that some of the HF patients might have a reductive stress (RS) condition. Investigating RS-related mechanisms will aid in personalized optimization of redox homeostasis for better outcomes among HF patients. METHODS: Blood samples were collected from HF patients (n = 54) and healthy controls (n = 42) and serum was immediately preserved in - 80 °C for redox analysis...
May 18, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29775931/a-nanocomposite-optosensor-containing-carboxylic-functionalized-multiwall-carbon-nanotubes-and-quantum-dots-incorporated-into-a-molecularly-imprinted-polymer-for-highly-selective-and-sensitive-detection-of-ciprofloxacin
#7
Naphat Yuphintharakun, Piyaluk Nurerk, Kochaporn Chullasat, Proespichaya Kanatharana, Frank Davis, Dhassida Sooksawat, Opas Bunkoed
A nanocomposite optosensor consisting of carboxylic acid functionalized multiwall carbon nanotubes and CdTe quantum dots embedded inside a molecularly imprinted polymer (COOH@MWCNT-MIP-QDs) was developed for trace ciprofloxacin detection. The COOH@MWCNT-MIP-QDs were synthesized through a facile sol-gel process using ciprofloxacin as a template molecule, 3-aminopropylethoxysilane as a functional monomer and tetraethoxysilane as a cross-linker at a molar ratio of 1:8:20. The synthesized nanocomposite optosensor had high sensitivity, excellent specificity and high binding affinity to ciprofloxacin...
May 10, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29775867/phytochemical-profiles-phototoxic-and-antioxidant-properties-of-eleven-hypericum-species-a-comparative-study
#8
Edoardo Napoli, Laura Siracusa, Giuseppe Ruberto, Alessandra Carrubba, Silvia Lazzara, Antonio Speciale, Francesco Cimino, Antonella Saija, Mariateresa Cristani
Hypericum is one out of the nine genera belonging to the botanical family Clusiaceae Lindl (syn. Hypericaceae Juss.; APG III, 2009). The genus contains 484 species spread worldwide, one of which, Hypericum perforatum, is largely used in folk medicine. The aim of this study was to evaluate the chemical composition, along with the antioxidant and phototoxic activity, of 11 Hypericum species grown in Sicily (H. perforatum L., H. aegypticum L., H. androsaemum L., H. calycinum L., H. hircinum L., H. hirsutum L...
May 15, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29775773/trimeprazine-is-enantioselectively-degraded-by-an-activated-sludge-in-ready-biodegradability-test-conditions
#9
Laura Escuder-Gilabert, Yolanda Martín-Biosca, Mireia Perez-Baeza, Salvador Sagrado, María José Medina-Hernández
A great number of available pharmaceuticals are chiral compounds. Although they are usually manufactured as racemic mixtures, they can be enantioselectively biodegraded as a result of microbial processes. In this paper, a biodegradability assay in similar conditions to those recommended in OECD tests of enantiomers of trimeprazine (a phenothiazine employed as a racemate) is carried out. Experiments were performed in batch mode using a minimal salts medium inoculated with an activated sludge (collected from a Valencian Waste Water Treatment Plant, WWTP) and supplemented with the racemate...
May 9, 2018: Water Research
https://www.readbyqxmd.com/read/29775308/piperidine-alkaloids-with-diverse-skeletons-from-anacyclus-pyrethrum
#10
Qi-Bin Chen, Jie Gao, Guo-An Zou, Xue-Lei Xin, Haji Akber Aisa
Fifteen new piperidine derivatives, pyracyclumines A-J (1-10), including five pairs of enantiomers, (+)-1/(-)-1 to (+)-5/(-)-5, together with three known compounds, agrocybenine (11), 4,6,6-trimethyl-5,6-dihydro-2(1 H)-pyridone (12), and 3,5,5-trimethyl-1,5-dihydro-2 H-pyrrol-2-one (13), were isolated from the roots of Anacyclus pyrethrum. Pyracyclumines A, B, and H (1, 2, and 8) possess a novel 6/5/6/6 dimeric piperidine skeleton, a unique 6/5/6 dimeric piperidine skeleton, and a 1,4,6-triazaindan skeleton, respectively...
May 18, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29775221/reagent-induced-asymmetric-induction-in-addition-reaction-of-reduced-glutathione-onto-bis-mannich-chalcones
#11
Aline Bernardes, Giulio D C D'Oliveira, Aneta Silezin, Mónika Kuzma, Szilárd Molnár, Caridad Noda Pérez, Pál Perjési
The stereochemistry of non-enzyme catalyzed nucleophilic addition of GSH to 4'-hydroxychalcone 1 and its bis-Mannich derivative 2 was investigated at different pH values (pH 3.2, 6.1, 7.4, and 8.0). The stereochemical outcome of the reactions was evaluated by HPLC-UV-Vis method. Under strongly acidic conditions (pH 3.2), an unexpected diastereoselective addition of GSH onto the bis-Mannich derivative 2 was observed. Such a selectivity could not be observed in the similar reaction of 2 with N-acetylcysteine...
May 18, 2018: Archiv der Pharmazie
https://www.readbyqxmd.com/read/29775200/cadmium-induced-changes-in-the-production-of-siderophores-by-a-plant-growth-promoting-strain-of-pseudomonas-fulva
#12
Dominika Thiem, Michał Złoch, Renata Gadzała-Kopciuch, Sonia Szymańska, Christel Baum, Katarzyna Hrynkiewicz
The impact of increasing Cd2+ exposure on the quality and quantity of siderophores produced by a plant growth promoting Pseudomonas fulva strain was tested to gain insight into the degree of change. P. fulva was cultured in the increasing concentrations of Cd2+ (0, 0.5, 1.0, 2.0 mM). The secreted siderophores were separated by HPLC and characterized by UHPLC-QTOF/MS. In the presence of 2 mM Cd2+ synthesis of siderophores (hydroxamates, catecholates, phenolates) was mitigated compared to the treatments with lower concentrations of Cd2+ (0...
May 18, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29775069/simultaneous-release-and-labelling-of-o-and-n-glycans-allowing-for-rapid-glycomic-analysis-by-online-lc-uv-esi-ms-ms
#13
Chengjian Wang, Yu Lu, Jianli Han, Wanjun Jin, Lingmei Li, Ying Zhang, Xuezheng Song, Linjuan Huang, Zhongfu Wang
Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming. Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed. Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labelled in situ and thus available for further characterization. In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by beta-elimination and N-glycans liberated by alkaline hydrolysis...
May 18, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29774074/placenta-specific-drug-delivery-by-trophoblast-targeted-nanoparticles-in-mice
#14
Baozhen Zhang, Lunbo Tan, Yan Yu, Baobei Wang, Zhilong Chen, Jinyu Han, Mengxia Li, Jie Chen, Tianxia Xiao, Balamurali K Ambati, Lintao Cai, Qing Yang, Nihar R Nayak, Jian Zhang, Xiujun Fan
Rationale: The availability of therapeutics to treat pregnancy complications is severely lacking, mainly due to the risk of harm to the fetus. In placental malaria, Plasmodium falciparum- infected erythrocytes (IEs) accumulate in the placenta by adhering to chondroitin sulfate A (CSA) on the surfaces of trophoblasts. Based on this principle, we have developed a method for targeted delivery of payloads to the placenta using a synthetic placental CSA-binding peptide (plCSA-BP) derived from VAR2CSA, a CSA-binding protein expressed on IEs...
2018: Theranostics
https://www.readbyqxmd.com/read/29774035/aldaulactone-an-original-phytotoxic-secondary-metabolite-involved-in-the-aggressiveness-of-alternaria-dauci-on-carrot
#15
Julia Courtial, Latifa Hamama, Jean-Jacques Helesbeux, Mickaël Lecomte, Yann Renaux, Esteban Guichard, Linda Voisine, Claire Yovanopoulos, Bruno Hamon, Laurent Ogé, Pascal Richomme, Mathilde Briard, Tristan Boureau, Séverine Gagné, Pascal Poupard, Romain Berruyer
Qualitative plant resistance mechanisms and pathogen virulence have been extensively studied since the formulation of the gene-for-gene hypothesis. The mechanisms involved in the quantitative traits of aggressiveness and plant partial resistance are less well-known. Nevertheless, they are prevalent in most plant-necrotrophic pathogen interactions, including the Daucus carota - Alternaria dauci interaction. Phytotoxic metabolite production by the pathogen plays a key role in aggressiveness in these interactions...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29774015/comparative-proteomic-and-morphological-change-analyses-of-staphylococcus-aureus-during-resuscitation-from-prolonged-freezing
#16
Biao Suo, Hua Yang, Yuexia Wang, Haipeng Lv, Zhen Li, Chao Xu, Zhilu Ai
When frozen, Staphylococcus aureus survives in a sublethally injured state. However, S. aureus can recover at a suitable temperature, which poses a threat to food safety. To elucidate the resuscitation mechanism of freezing survived S. aureus , we used cells stored at -18°C for 90 days as controls. After resuscitating the survived cells at 37°C, the viable cell numbers were determined on tryptic soy agar with 0.6% yeast extract (TSAYE), and the non-injured-cell numbers were determined on TSAYE supplemented with 10% NaCl...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29772937/evaluation-of-sulphonamide-derivatives-acting-as-inhibitors-of-human-carbonic-anhydrase-isoforms-i-ii-and-mycobacterium-tuberculosis-%C3%AE-class-enzyme-rv3273
#17
Tanvi V Wani, Silvia Bua, Pravin S Khude, Abdul H Chowdhary, Claudiu T Supuran, Mrunmayee P Toraskar
A series of novel sulphonamide derivatives was obtained from sulphanilamide which was N4-alkylated with ethyl bromoacetate followed by reaction with hydrazine hydrate. The hydrazide obtained was further reacted with various aromatic aldehydes. The novel sulphonamides were characterised by infrared, mass spectrometry, 1 H- and 13 C-NMR and purity was determined by high-performance liquid chromatography (HPLC). Human (h) carbonic anhydrase (CA, EC 4.2.1.1) isoforms hCA I and II and Mycobacterium tuberculosis β-CA encoded by the gene Rv3273 (mtCA 3) inhibition activity was investigated with the synthesised compounds which showed promising inhibition...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/29772610/application-of-liquid-liquid-microextraction-for-the-effective-separation-and-simultaneous-determination-of-11-pharmaceuticals-in-wastewater-samples-using-hplc-ms-ms
#18
Alina Diuzheva, József Balogh, József Jekő, Zoltán Cziáky
A dispersive liquid-liquid microextraction method for the simultaneous determination of 11 pharmaceuticals has been developed. The method is based on a microextraction procedure applied to wastewater samples from different regions of Hungary followed by high performance liquid chromatography with mass spectrometry. The effect of the nature of the extractant, dispersive solvent, different additives and extraction time were examined on the extraction efficiently of the dispersive liquid-liquid microextraction method...
May 17, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29772601/hollow-mesoporous-structured-molecularly-imprinted-polymer-as-adsorbent-in-pipette-tip-solid-phase-extraction-for-the-determination-of-antiretrovirals-from-plasma-of-hiv-infected-patients
#19
Nathália Soares Simões, Hanna Leijoto de Oliveira, Ricky Cássio Santos da Silva, Leila Suleimara Teixeira, Thaís Lorenna Souza Sales, Whocely Victor de Castro, Maria José Nunes de Paiva, Cristina Sanches, Keyller Bastos Borges
In this work a hollow mesoporous structured molecularly imprinted polymer was synthetized and used as adsorbent in pipette-tip solid-phase extraction for the determination of lamivudine (3TC), zidovudine (AZT) and efavirenz (EFZ) from plasma of human immunodeficiency virus (HIV) infected patients by high-performance liquid chromatography (HPLC). All parameters that influence the recovery of the pipette tip based on hollow mesoporous molecularly imprinted polymer solid-phase extraction (PT-HM-MIP-SPE) method were systematically studied and discussed in detail...
May 17, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29772520/-valeriana-wallichii-extract-inhibits-tert-booh-induced-oxidative-damage-and-cytotoxicity
#20
Vishal Jain, Ruma Dutta, Doddamane Thippeswamy Maheshwari, Darmendra Kumar Meena, Kshipra Misra, Mysore Sadashiva Yogendra Kumar
Oxidative s tress is well known to be involved in pathophysiology of several disorders. Valeriana wallichii (VW) root extracts is known for its expedient activities but its antioxidant and cytoprotective efficacy need to be explored further. In present study tertiary-butyl hydroperoxide (tert-BOOH) was used to induce oxidative stress in C6 glioma cells. Antioxidant activity of the VW root extracts were evaluated by chemical assays i.e . DPPH, ABTS and FRAP assay. Further effect of VW on tert-BOOH induced oxidative stress and mitochondrial damage was studied...
June 1, 2018: Frontiers in Bioscience (Elite Edition)
keyword
keyword
2759
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"