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https://www.readbyqxmd.com/read/28927121/the-role-of-jagged1-notch-pathway-mediated-angiogenesis-of-hepatocarcinoma-cells-in-vitro-and-the-effects-of-the-spleen-invigorating-and-blood-stasis-removing-recipe
#1
Juze Lin, Yongxin Lin, Le Su, Qiao Su, Wei Guo, Xuhui Huang, Changjun Wang, Lizhu Lin
The objective of this study was to observe the effect of Jagged1/Notch pathway-mediated angiogenesis on the in vitro proliferation of hepatocellular carcinoma cells, and the effect and possible mechanism of the spleen-invigorating and blood stasis-removing recipe. Spleen-invigorating and blood stasis-removing recipe serum from SPF grade nude mice was prepared, and the fingerprint of the drugs of the spleen-invigorating and blood stasis-removing recipe and drug serum were identified by HPLC. SMMC-7721 human hepatocellular carcinoma cells were divided into the normal control group, DAPT inhibitor control group, and drug serum group according to the different treatments...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926992/the-native-fruit-geoffroea-decorticans-from-arid-northern-chile-phenolic-composition-antioxidant-activities-and-in-vitro-inhibition-of-pro-inflammatory-and-metabolic-syndrome-associated-enzymes
#2
Felipe Jiménez-Aspee, Cristina Theoduloz, Maria Del Pilar C Soriano, Maider Ugalde-Arbizu, Maria Rosa Alberto, Iris Catiana Zampini, Maria Inés Isla, Mario J Simirigiotis, Guillermo Schmeda-Hirschmann
The native tree Geoffroea decorticans (chañar) grows in the arid lands of northern Chile. It has been used as a food plant since prehistoric times. Phenolic-enriched extracts (PEEs) of Chilean chañar fruits were assessed for their chemical composition, antioxidant properties and inhibition of pro-inflammatory and metabolic syndrome-associated enzymes. Phenolic profiles were determined by HPLC-DAD-MS/MS. The PEEs of G. decorticans showed a strong effect towards the enzymes COX-1/COX-2, with inhibition percentages ranging from inactive to 92...
September 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28926841/beneficial-combination-of-lacosamide-with-retigabine-in-experimental-animals-an-isobolographic-analysis
#3
Jarogniew J Luszczki, Mirosław Zagaja, Barbara Miziak, Maria W Kondrat-Wrobel, Katarzyna Zaluska, Paula Wroblewska-Luczka, Piotr Adamczuk, Stanislaw J Czuczwar, Magdalena Florek-Luszczki
BACKGROUND/AIM: To isobolographically determine the types of interactions that occur between retigabine and lacosamide (LCM; two third-generation antiepileptic drugs) with respect to their anticonvulsant activity and acute adverse effects (sedation) in the maximal electroshock-induced seizures (MES) and chimney test (motor performance) in adult male Swiss mice. METHODS: Type I isobolographic analysis for nonparallel dose-response effects for the combination of retigabine with LCM (at the fixed-ratio of 1:1) in both the MES and chimney test in mice was performed...
September 20, 2017: Pharmacology
https://www.readbyqxmd.com/read/28926799/iron-deficiency-stimulates-anthocyanin-accumulation-in-grapevine-apical-leaves
#4
Leila Caramanico, Laura Rustioni, Gabriella De Lorenzis
Iron chlorosis is a diffuse disorder affecting Mediterranean vineyards. Beside the commonly described symptom of chlorophyll decrease, an apex reddening was recently observed. Secondary metabolites, such as anthocyanins, are often synthetized to cope with stresses in plants. The present work aimed to evaluate grapevine responses to iron deficiency, in terms of anthocyanin metabolism (reflectance spectrum, total anthocyanin content, HPLC profile and gene expression) in apical leaves of Cabernet sauvignon and Sangiovese grown in hydroponic conditions...
September 9, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28926735/ketamine-metabolites-with-antidepressant-effects-fast-economical-and-eco-friendly-enantioselective-separation-based-on-supercritical-fluid-chromatography-sfc-and-single-quadrupole-ms-detection
#5
Georg M Fassauer, Robert Hofstetter, Mahmoud Hasan, Stefan Oswald, Christina Modeß, Werner Siegmund, Andreas Link
Increasing evidence accumulates that metabolites of the dissociative anesthetic ketamine contribute considerably to the biological effects of this drug and could be developed as next generation antidepressants, especially for acute treatment of patients with therapy-refractory major depression. Analytical methods for the simultaneous determination of the plethora of hydroxylated, dehydrogenated and/or demethylated compounds formed after administration of ketamine hydrochloride are a prerequisite for future clinical investigations and a deeper understanding of the individual role of the isomers of these metabolites...
September 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28926678/organic-polymer-based-monolithic-stationary-phases-with-incorporated-nanostructured-materials-for-hplc-and-cec
#6
REVIEW
Nisansala Ganewatta, Ziad El Rassi
This review article is concerned with the recent advances made in the field of organic polymer-based monoliths with incorporated nanostructured materials (NSMs) for use in liquid chromatography and capillary electrochromatography. It covers the pertinent literature published over the last 7-8 years with a total of 56 references. The present article has two distinct parts: one major part encompassing "traditional" organic polymer-based monoliths modified with NSMs and a minor part on cryogels modified with NSMs...
September 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28926513/pharmacokinetics-and-safety-profile-of-single-dose-administration-of-an-estrogen-receptor-%C3%AE-selective-phytoestrogenic-phytoserm-formulation-in-perimenopausal-and-postmenopausal-women
#7
Gerson Hernandez, Liqin Zhao, Adrian A Franke, Yu-Ling Chen, Wendy J Mack, Roberta D Brinton, Lon S Schneider
OBJECTIVE: Selected estrogen receptor β-selective phytoestrogen (phytoSERM), a preparation of genistein, daidzein, and S-equol, has an 83-fold selective affinity for estrogen receptor (ER) β, and may promote neuronal survival and estrogenic mechanisms in the brain without exerting feminizing activity in the periphery. The aim of this study was to assess the safety, tolerability, and single-dose pharmacokinetics of the phytoSERM formulation in perimenopausal and postmenopausal women...
September 18, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28926237/a-bis-benzopyrroloisoquinoline-alkaloid-incorporating-a-cyclobutane-core-and-a-chlorophenanthroindolizidine-alkaloid-with-cytotoxic-activity-from-ficus-fistulosa-var-tengerensis
#8
Amjad Ayad Qatran Al-Khdhairawi, Premanand Krishnan, Chun-Wai Mai, Felicia Fei-Lei Chung, Chee-Onn Leong, Kien-Thai Yong, Kam-Weng Chong, Yun-Yee Low, Toh-Seok Kam, Kuan-Hon Lim
Tengerensine (1), isolated as a racemate and constituted from a pair of bis-benzopyrroloisoquinoline enantiomers, and tengechlorenine (2), purified as a scalemic mixture and constituted from a pair of chlorinated phenanthroindolizidine enantiomers, were isolated from the leaves of Ficus fistulosa var. tengerensis, along with three other known alkaloids. The structures of 1 and 2 were determined by spectroscopic data interpretation and X-ray diffraction analysis. The enantiomers of 1 were separated by chiral-phase HPLC, and the absolute configurations of (+)-1 and (-)-1 were established via experimental and calculated ECD data...
September 19, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28925879/human-fibronectin-extra-domain-b-edb-speci%C3%AF-c-aptide-aptedb-radiolabelling-with-technetium-99m-as-a-potent-targeted-tumour-imaging-agent
#9
Mohsen Mohammadgholi, Nourollah Sadeghzadeh, Mostafa Erfani, Saeid Abediankenari, Seyed Mohammad Abedi, Iman Emrarian, Narjes Jafari, Ramezan Behzadi
BACKGROUND: Human fibronectin extra-domain B (EDB) is particularly expressed during angiogenesis progression. It is, thus, a promising marker of tumour growth. Aptides are a novel class of peptides with high-affinity binding to specific protein targets. APTEDB is an antagonist-like ligand that especially interacts with human fibronectin EDB. OBJECTIVE: This study was the first attempt in which the hydrazinonicotinamide (HYNIC)-conjugated APTEDB was labelled with technetium-99m (99mTc) as an appropriate radiotracer and tricine/EDDA exchange labeling ...
September 18, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28925715/novel-carbon-dots-derived-from-schizonepetae-herba-carbonisata-and-investigation-of-their-haemostatic-efficacy
#10
Meiling Zhang, Yan Zhao, Jinjun Cheng, Xiaoman Liu, Yongzhi Wang, Xin Yan, Yue Zhang, Fang Lu, Qingguo Wang, Huihua Qu
Schizonepetae Herba Carbonisata (SHC) has been used in traditional Chinese medicine (TCM) to treat haemorrhagic diseases for more than 1000 years. However, little information is available on its haemostatic components and mechanism. In this study, we developed novel water-soluble carbon dots (CDs) in aqueous extracts of SHC for the first time and a modified pyrolysis method was used to prepare the SHC using Schizonepetae Herba (SH) as the sole precursor. The SHC-CDs were characterized using transmission electron microscopy (TEM), high-resolution TEM (HRTEM), Fourier transform infrared (FT-IR), ultraviolet-visible (UV-Vis) and fluorescence spectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and high-performance liquid chromatography (HPLC)...
September 19, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28925291/isolation-of-a-novel-compound-mimo2-from-the-methanolic-extract-of-moringa-oleifera-leaves-protective-effects-against-vanadium-induced-cytotoxity
#11
Olumayowa O Igado, Jan Glaser, Mario Ramos-Tirado, Ezgi Eylül Bankoğlu, Foluso A Atiba, Ulrike Holzgrabe, Helga Stopper, James O Olopade
Moringa oleifera is reported to be a miracle plant, with positive effects on practically every system in the animal body. The methanolic extract of Moringa oleifera leaves was fractionated using liquid-liquid fractionation, column chromatography and preparative high-performance liquid chromatography (HPLC). Bioassay guided fractionation using Ferric Reducing Antioxidant Power (FRAP) was used to determine the fraction with the highest antioxidative power. Chemical structure was elucidated with nuclear magnetic resonance (NMR) spectroscopy...
September 19, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28925167/-analysis-on-intestinal-absorption-of-paeoniflorin-lipid-liquid-crystalline-nanoparticles-via-everted-intestinal-sacs
#12
Shuang Teng, Ling Qiu, Hong-Ge Chen, Bao-de Shen, Cheng-Ying Shen, Ping-Hua Xu, Jin Han, Hai-Long Yuan
To study the absorption kinetics of paeoniflorin lipid liquid crystalline nanoparticles (Pae-LLCN) in different intestinal segments of rats and compare them with paeoniflorin(Pae) solution. Rat everted gut sac models were adopted for intestinal absorption test, and Pae content was determined by HPLC method to study the absorption characteristics of Pae-LLCN in rat duodenum, jejunum, ileum and colon, and investigate the effects of different drug concentrations on intestinal absorption. Results showed that Pae-LLCN and Pae were well absorbed at different intestine segments and different concentrations...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925166/-comparison-of-pharmacokinetics-of-curcumin-in-rats-administered-with-two-kinds-of-polymeric-micelles
#13
Di Zhang, Qian Xu, Ke Liu, Hui Xu
To investigate the effect of end-capped modification of mPEG-PLA with Boc-phenylalanine(BP) on pharmacokinetic characteristics of curcumin(CUR) loaded micelles, and then provide experimental evidence for prescription optimization. Healthy male SD rats were randomly divided into three groups and they were intravenously administered with a single injection of CUR-mPEG-PLA micelles, CUR-mPEG-PLA-BP micelles and reference formulations DMSO solution(n=6). The doses were 20 mg•kg⁻¹ in term of CUR. Blood samples were collected before and after administration, and the concentration of curcumin in blood plasma was determined by HPLC to draw time-concentration curve...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925158/-study-on-chemical-constituents-of-seeds-of-croton-tiglium-and-their-cytotoxicities
#14
Hai-Guo Su, Huai Yang, Chun-Wang Meng, Cheng Peng, Li Guo, Ou Dai
Seven compounds were isolated from the seeds of Croton tiglium by preparative TLC, semi-preparative HPLC, and column chromatography over silica gel, MCI, and Sephadex LH-20, etc. Their structures were elucidated by spectroscopic data analysis as bis(2,3-dihydroxypropyl) nonanedioate (1), 12-O-(α-methyl)butyrylphorbol-13-decanoate (2), 12-O-tiglylphorbol-13-decanoate (3), (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoic acid (4), methyl (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoate (5), 4(1H)-quinolinone (6), and 5-hydroxy-2-pyridinemethanol (7)...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925149/-research-on-modeling-method-to-analyze-lonicerae-japonicae-flos-extraction-process-with-online-mems-nir-based-on-two-types-of-error-detection-theory
#15
Chen-Zhao Du, Zhi-Sheng Wu, Na Zhao, Zheng Zhou, Xin-Yuan Shi, Yan-Jiang Qiao
To establish a rapid quantitative analysis method for online monitoring of chlorogenic acid in aqueous solution of Lonicera Japonica Flos extraction by using micro-electromechanical near infrared spectroscopy (MEMS-NIR). High performance liquid chromatography(HPLC) was used as reference method.Kennard-Stone (K-S) algorithm was used to divide sample sets, and partial least square(PLS) regression was adopted to establish the multivariate analysis model between the HPLC analysis contents and NIR spectra. The synergy interval partial least squares (SiPLS) was used to selected modeling waveband to establish PLS models...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925148/-near-infrared-analysis-of-blending-homogeneity-of-chinese-medicine-formula-particles-based-on-moving-window-f-test-method
#16
Chan Yang, Bing Xu, Zhi-Qiang Zhang, Xin Wang, Xin-Yuan Shi, Jing Fu, Yan-Jiang Qiao
Blending uniformity is essential to ensure the homogeneity of Chinese medicine formula particles within each batch. This study was based on the blending process of ebony spray dried powder and dextrin(the proportion of dextrin was 10%),in which the analysis of near infrared (NIR) diffuse reflectance spectra was collected from six different sampling points in combination with moving window F test method in order to assess the blending uniformity of the blending process.The method was validated by the changes of citric acid content determined by the HPLC...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925147/-analysis-of-different-forms-linderae-radix-based-on-hplc-and-nirs-fingerprints
#17
Wei-Feng Du, Xian-Ke Yue, Yao Wu, Wei-Hong Ge, Tu-Lin Lu, Zhi-Min Wang
Three different forms of Linderae Radix were evaluated by HPLC combined with NIRS fingerprint. The Linderae Radix was divided into three forms, including spindle root, straight root and old root. The HPLC fingerprints were developed, and then cluster analysis was performed using the SPSS software. The near-infrared spectra of Linderae Radix was collected, and then established the discriminant analysis model. The similarity values of the spindle root and straight root all were above 0.990, while the similarity value of the old root was less than 0...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925146/-rapid-monitoring-five-components-of-ethanol-precipitation-process-of-shenzhiling-oral-solution-using-near-infrared-spectroscopy
#18
Tong-Tong Li, Tian Hu, Lei Nie, Li-Xuan Zang, Ying-Zi Zeng, Heng-Chang Zang
To develop a method for the rapid monitoring of five components during the alcohol precipitation process of Shenzhiling oral solution using near infrared spectroscopy(NIRS).The contents of five components detemined by high performance liquid chromatography(HPLC) were used as the reference values, and the NIRS based partial least square regression(PLSR) models were used to monitor the concentrations of paeoniflorin, albiflorin, liquiritin, cinnamic acid and glycyrrhizic acid during the alcohol precipitation process of Shenzhiling oral solution, which were optimized and verified through comparing of different spectral pre-processing and variables selection methods...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925046/in-vivo-pharmacodynamic-and-pharmacokinetic-interactions-of-hibiscus-sabdariffa-calyces-extracts-with-simvastatin
#19
S J Showande, O M Adegbolagun, S I Igbinoba, T O Fakeye
WHAT IS KNOWN AND OBJECTIVES: Increasing number of patients use herbs with their medications. Such practice may result in beneficial or harmful herb-drug interactions. A recent survey reported that some participants co-administered Hibiscus sabdariffa, a widely used beverage, or tea, with their antihyperlipidaemic medications. This study therefore evaluated the effect of concomitant administration of Hibiscus sabdariffa calyces' extracts with simvastatin on hyperlipidaemia and pharmacokinetics of the drug in vivo...
September 18, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28925027/a-prospective-international-study-on-adherence-to-treatment-in-305-patients-with-flaring-sle-assessment-by-drug-levels-and-by-self-administered-questionnaires
#20
Nathalie Costedoat-Chalumeau, Frédéric Houssiau, Peter Izmirly, Véronique Le Guern, Sandra Navarra, Meenakshi Jolly, Guillermo Ruiz-Irastorza, Gabriel Baron, Eric Hachulla, Nancy Agmon-Levin, Yehuda Shoenfeld, Francesca Dall'Ara, Jill Buyon, Christophe Deligny, Ricard Cervera, Estibaliz Lazaro, Holy Bezanahary, Gaëlle Leroux, Nathalie Morel, Jean-François Viallard, Christian Pineau, Lionel Galicier, Ronald Van Vollenhoven, Angela Tincani, Hanh Nguyen, Guillaume Gondran, Noel Zahr, Jacques Pouchot, Jean-Charles Piette, Michelle Petri, David Isenberg
Nonadherence to treatment is a major cause of lupus flares. Hydroxychloroquine (HCQ), a major medication in systemic lupus erythematosus, has a long half-life and can be quantified by HPLC. This international study evaluated nonadherence in 305 lupus patients with flares using drug levels (HCQ<200ng/ml or undetectable desethylchloroquine), and self-administered questionnaires (MASRI<80% or MMAS-8<6). Drug levels defined 18.4% of the patients as severely nonadherent. In multivariate analyses, younger age, non-use of steroids, higher BMI and unemployment were associated with nonadherence by drug level...
September 19, 2017: Clinical Pharmacology and Therapeutics
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