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https://www.readbyqxmd.com/read/28073396/nonomuraea-ceibae-sp-nov-a-new-actinobacterium-isolated-from-ceiba-speciosa-rhizosphere
#1
Fei Wang, Jindi Shi, Yaojian Huang, Yingying Wu, Xianming Deng
Strain XMU 110T, isolated from the rhizosphere soil of a flowering tree, Ceiba speciosa, was characterized by polyphasic taxonomy. Phylogenetic analysis based on the 16S rRNA gene comparisons revealed that strain XMU 110T showed the highest similarity of 97.9 % to Nonomuraea jabiensis DSM 45507T, and indicated the closest relatives of strain Nonomuraea roseoviolacea subsp. roseoviolacea ATCC 27297T (97.8 %) and Nonomuraea salmonea DSM 43678T (97.4 %) after a neighbour-joining analysis. The phenotypic characteristics, as well as the levels of DNA-DNA relatedness between strain XMU 110T and N...
January 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28061749/organelle_pba-a-pipeline-for-assembling-chloroplast-and-mitochondrial-genomes-from-pacbio-dna-sequencing-data
#2
Aboozar Soorni, David Haak, David Zaitlin, Aureliano Bombarely
BACKGROUND: The development of long-read sequencing technologies, such as single-molecule real-time (SMRT) sequencing by PacBio, has produced a revolution in the sequencing of small genomes. Sequencing organelle genomes using PacBio long-read data is a cost effective, straightforward approach. Nevertheless, the availability of simple-to-use software to perform the assembly from raw reads is limited at present. RESULTS: We present Organelle-PBA, a Perl program designed specifically for the assembly of chloroplast and mitochondrial genomes...
January 7, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28056394/facile-biosynthesis-characterization-and-solar-assisted-photocatalytic-effect-of-zno-nanoparticles-mediated-by-leaves-of-l-speciosa
#3
V Sai Saraswathi, J Tatsugi, Paik-Kyun Shin, K Santhakumar
Synthesis of metal oxide nanoparticles using novel methodologies always attracts great importance in research. The use of plant extract to synthesize nano-particle has been considered as one of the eco-friendly methods. This paper describes the biosynthetic route of preparation of zinc oxide nanoparticles (ZnO NPs) from the Lagerstroemia speciosa leaf extract. This approach appears to be low-cost preparation and alternative method to conventional methods. Highly stable and hexagonal phase ZnO NPs with average particle size of 40nm were synthesized and characterized by UV-Vis absorption spectroscopy (surface Plasmon resonance), Fourier transform infrared spectroscopy (surface functionalities), X-ray Diffraction analysis (crystallinity), TEM and SEM (size and morphology), Energy Dispersive X-ray spectroscopy (elemental composition), Thermogravimetric analysis (weight loss) and Zeta potential (stability)...
December 28, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28027944/antibiofilm-efficacy-of-green-synthesized-graphene-oxide-silver-nanocomposite-using-lagerstroemia-speciosa-floral-extract-a-comparative-study-on-inhibition-of-gram-positive-and-gram-negative-biofilms
#4
Shatavari Kulshrestha, Shariq Qayyum, Asad U Khan
Biofilm architecture provides bacteria with enhanced antibiotic resistance, thus raising the need to search for alternative therapies that can inhibit the bacterial colonization. In the present study, we synthesized graphene oxide-silver nanocomposite (GO-Ag) by non-toxic and eco-friendly route using a floral extract of Legistromia speciosa (L.) Pers. The gas chromatography-mass spectrometry (GC-MS) analysis of plant extract revealed the presence of compounds which can simultaneously act as reducing and capping agents...
December 24, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28027593/behavior-of-sheep-fed-babassu-cake-orbygnia-speciosa-as-a-substitution-for-elephant-grass-silage
#5
Ernestina Ribeiro Dos Santos Neta, Kaliandra Souza Alves, Rafael Mezzomo, Daiany Íris Gomes, Luis Rennan Sampaio Oliveira, Francisco Fernando Ramos de Carvalho, Janaina Barros Luz, Natália Gomes Lacerda, Venucia de Diniella Dos Santos Bourdon
This study aimed to evaluate the behavior of sheep fed babassu cake as a substitution for elephant grass silage. The experiment was conducted at the Federal Rural University of Amazonia (Universidade Federal Rural da Amazônia - UFRA), Brazil, using 45 sheep housed in individual stalls, with unlimited access to feed and distributed in a completely randomized design with five treatments (0, 12.5, 25, 37.5 and 50% inclusion of babassu cake) and nine replications. All of the treatments contained a roughage-to-concentrate ratio of 40:60...
December 27, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27999544/mitragyna-speciosa-leaf-extract-exhibits-antipsychotic-like-effect-with-the-potential-to-alleviate-positive-and-negative-symptoms-of-psychosis-in-mice
#6
Kamini Vijeepallam, Vijayapandi Pandy, Thubasni Kunasegaran, Dharmani D Murugan, Murali Naidu
In this study, we investigated the antipsychotic-like effect of methanolic extract of Mitragyna speciosa leaf (MMS) using in vivo and ex vivo studies. In vivo studies comprised of apomorphine-induced climbing behavior, haloperidol-induced catalepsy, and ketamine-induced social withdrawal tests in mice whereas the ex vivo study was conducted utilizing isolated rat vas deferens preparation. Acute oral administration of MMS (50-500 mg/kg) showed an inverted bell-shaped dose-response in apomorphine-induced cage climbing behavior in mice...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27951469/-evaluation-of-ion-mobility-spectrometry-for-the-detection-of-mitragynine-in-kratom-products
#7
Nathan Fuenffinger, Melissa Ritchie, Ashley Ruth, Connie Gryniewicz-Ruzicka
An ion mobility spectrometry (IMS) method was developed for the rapid detection of mitragynine, the most abundant alkaloid in Mitragyna speciosa also known as kratom. The peak corresponding to the mitragynine protonated ion exhibited a reduced ion mobility of 0.95±0.00014cm(2)/(Vs), and the mitragynine limit of detection using IMS was 0.5ng. The IMS method was applied to the analysis of 15 commercial samples suspected of containing kratom. IMS results were compared to those obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the same samples...
February 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27907185/antioxidant-antimicrobial-and-cytotoxic-properties-as-well-as-the-phenolic-content-of-the-extract-from-hancornia-speciosa-gomes
#8
Uilson P Santos, Jaqueline F Campos, Heron Fernandes V Torquato, Edgar Julian Paredes-Gamero, Carlos Alexandre Carollo, Leticia M Estevinho, Kely de Picoli Souza, Edson Lucas Dos Santos
Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death...
2016: PloS One
https://www.readbyqxmd.com/read/27893147/update-on-the-pharmacology-and-legal-status-of-kratom
#9
Walter C Prozialeck
Kratom (Mitragyna speciosa) is a plant indigenous to Southeast Asia. Its leaves and the teas brewed from them have long been used by people in that region to stave off fatigue and to manage pain and opioid withdrawal. In a comprehensive review published in 2012, Prozialeck et al presented evidence that kratom had been increasingly used for the self-management of opioid withdrawal and pain in the United States. At the time, kratom was classified as a legal herbal product by the US Drug Enforcement Administration...
December 1, 2016: Journal of the American Osteopathic Association
https://www.readbyqxmd.com/read/27861139/in-vitro-antioxidant-and-anti-inflammatory-activities-of-isolated-compounds-of-ethanol-extract-from-sanchezia-speciosa-leonard-s-leaves
#10
Tung Bui Thanh, Loi Vu Duc, Hai Nguyen Thanh, Vung Nguyen Tien
BACKGROUND: Sanchezia speciosa has been used in traditional medicine for gastritis treatment in Vietnam. Some phytochemical study showed that S. speciosa contains high amounts of flavonoids and cardiac glycoside compounds. In the present study, we isolated four compounds from the ethanol extract of the S. speciosa leaf, and we evaluated the in vitro antioxidant and anti-inflammatory effect of the isolated compound from the ethanol extract of the S. speciosa leaf. METHODS: The leaf of S...
January 1, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/27857843/green-synthesis-of-silver-nanoparticles-using-extract-of-parkia-speciosa-hassk-pods-assisted-by-microwave-irradiation
#11
Is Fatimah
This paper reports an investigation of the microwave-assisted synthesis of silver nanoparticles (Ag NPs) using extract of stinky bean (Parkia speciosa Hassk) pods (BP). The formation of Ag NPs was identified by instrumental analysis consists of UV-vis spectrophotometry, Fourier-transform infrared (FTIR) spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and particle size analysis. Furthermore, Ag NPs were used as antibacterial agents against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa...
November 2016: Journal of Advanced Research
https://www.readbyqxmd.com/read/27811992/eliciting-maize-defense-pathways-aboveground-attracts-belowground-biocontrol-agents
#12
Camila Cramer Filgueiras, Denis S Willett, Ramom Vasconcelos Pereira, Alcides Moino Junior, Martin Pareja, Larry W Duncan
Plant defense pathways mediate multitrophic interactions above and belowground. Understanding the effects of these pathways on pests and natural enemies above and belowground holds great potential for designing effective control strategies. Here we investigate the effects of aboveground stimulation of plant defense pathways on the interactions between corn, the aboveground herbivore adult Diabrotica speciosa, the belowground herbivore larval D. speciosa, and the subterranean ento-mopathogenic nematode natural enemy Heterorhabditis amazonensis...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27799032/herbal-highs-review-on-psychoactive-effects-and-neuropharmacology
#13
Silvia Graziano, Laura Orsolini, Maria Concetta Rotolo, R Tittarelli, Fabrizio Schifano, Simona Pichini
BACKGROUND: A new trend among users of new psychoactive substances' the consumption of "herbal highs": plant parts containing psychoactive substances. Most of the substances extracted from herbs, in old centuries were at the centre of religious ceremonies of ancient civilizations. Currently, these herbal products are mainly sold by internet web sites and easily obtained since some of them have no legal restriction. OBJECTIVE: We reviewed psychoactive effects and neuropharmacology of the most used "herbal highs" with characterized active principles, with studies reporting mechanisms of action, pharmacological and subjective effects, eventual secondary effects including intoxications and/or fatalities Method: The PubMed database was searched using the following key...
October 31, 2016: Current Neuropharmacology
https://www.readbyqxmd.com/read/27796451/rapid-classification-of-chinese-quince-chaenomeles-speciosa-nakai-fruit-provenance-by-near-infrared-spectroscopy-and-multivariate-calibration
#14
Wenhao Shao, Yanjie Li, Songfeng Diao, Jingmin Jiang, Ruxiang Dong
The quality of Chinese quince fruit is a significant factor for medicinal materials, influencing the quality of the medicine. However, it is difficult to distinguish different types of Chinese quince fruit. The main objective of this work was to use near-infrared (NIR) spectroscopy, which is a rapid and non-destructive analysis method, to classify the varieties of Chinese quince fruits. Raw spectra in the range of 1000 to 2500 nm were combined with linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), and support vector machines (SVMs) for classification...
January 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27768072/rapid-high-throughput-species-identification-of-botanical-material-using-direct-analysis-in-real-time-high-resolution-mass-spectrometry
#15
Ashton D Lesiak, Rabi A Musah
We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods...
October 2, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27752985/suspected-adulteration-of-commercial-kratom-products-with-7-hydroxymitragynine
#16
Alicia G Lydecker, Abhisheak Sharma, Christopher R McCurdy, Bonnie A Avery, Kavita M Babu, Edward W Boyer
INTRODUCTION: Kratom (Mitragyna speciosa), a plant native to Southeast Asia, has been used for centuries for its stimulant and opium-like effects. Mitragynine and 7-hydroxymitragynine, exclusive to M. speciosa, are the alkaloids primary responsible for Kratom's biologic and psychoactive profile, and likely contribute to its problematic use. We purchased several commercially available Kratom analogs for analysis and through our results, present evidence of probable adulteration with the highly potent and addictive plant alkaloid, 7-hydroxymitragynine...
December 2016: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/27725074/determination-of-mitragynine-in-mitragyna-speciosa-raw-materials-and-finished-products-by-liquid-chromatography-with-uv-detection-single-laboratory-validation
#17
Elizabeth M Mudge, Paula N Brown
Mitragyna speciosa (kratom) is a tree indigenous to Southeast Asia, and its leaves are used in herbal formulations because they contain indole alkaloids mitragynine and 7-hydroxy (7-OH) mitragynine. An HPLC method was developed, optimized, and validated using single-laboratory validation guidelines to quantify mitragynine in kratom raw materials and finished products. The method optimization evaluated several extraction parameters including solvent type, solvent volume, time, and extraction method. The separation of the mitragynine alkaloids was achieved in 18 min with a fused-core C18 EVO column using gradient separation with ammonium bicarbonate (pH 9...
October 8, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/27714492/carcass-characteristics-and-meat-quality-of-lambs-fed-babassu-cake-orbignya-speciosa-as-a-replacement-for-elephant-grass-silage
#18
Janaina Barros Luz, Kaliandra Souza Alves, Rafael Mezzomo, Ernestina Ribeiro Dos Santos Neta, Daiany Íris Gomes, Luis Rennan Sampaio Oliveira, Josiane Costa Silva, Francisco Fernando Ramos de Carvalho
The study aimed to evaluate the effect of the partial replacement of elephant grass silage with babassu cake (Orbignya speciosa) on the carcass characteristics and meat quality of feedlot lambs. Forty-five castrated male Santa Ines sheep (19.08 ± 0.41 kg) approximately 4 months old were distributed in a completely randomized design, with five treatments 0.0, 12.5, 25.0, 37.5 and 50 % (%DM) replacement of babassu cake with silage forming isoproteic diets formulated at a ratio of 40 % roughage to 60 % concentrate...
January 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27706595/genetic-diversity-in-natural-populations-of-mangaba-in-sergipe-the-largest-producer-state-in-brazil
#19
A N R Soares, M F Vitória, A L S Nascimento, A S Ledo, A R C Rabbani, A V C Silva
Mangaba (Hancornia speciosa Gomes) is found in areas of coastal tablelands in the Brazilian Northeast and Cerrado regions. This species has been subjected to habitat fragmentation that is mainly due to human activity, and requires conservation strategies. The aim of this study was to analyze the structure and inter- and intrapopulation genetic diversity of natural populations of H. speciosa Gomes using inter-simple sequence repeat (ISSR) molecular markers. A total of 155 individuals were sampled in 10 natural populations (ITA, PAC, IND, EST, BC, PIR, JAP, BG, NEO, and SANT) in the State of Sergipe, Brazil...
August 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27697029/fatty-acid-composition-of-mangrove-wild-legume-seeds-sesbania-speciosa-in-southwestern-india
#20
Kandikere R Sridhar, Dorathy D Anita, Sudeep D Ghate
This study compares the composition of fatty acid methyl esters (FAMEs) in seeds of Sesbania speciosa (dry and mature) after processing (uncooked and cooked) and extraction (hot and cold). Among PUFA, oleic, linoleic and linolenic acids were common to uncooked and cooked dry seeds which were high on cold extraction. Only two fatty acid ratios were favorable in hot extraction [(C14:0 + C15:0 + (C16:0 / C18:0) and C18:1 / C18:2]. Cold extraction yielded docosahexaenoic acid in uncooked as well as cooked dry seeds and all fatty acid ratios were nutritionally favorable...
October 3, 2016: Recent Patents on Food, Nutrition & Agriculture
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