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https://www.readbyqxmd.com/read/29329595/human-umbilical-cord-mesenchymal-stem-cell-conditioned-medium-attenuates-renal-fibrosis-by-reducing-inflammation-and-epithelial-to-mesenchymal-transition-via-the-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway-in-vivo-and-in-vitro
#1
Bo Liu, Fengxia Ding, Dong Hu, Yu Zhou, Chunlan Long, Lianju Shen, Yuanyuan Zhang, Deying Zhang, Guanghui Wei
BACKGROUND: Renal fibrosis is characterized by infiltration of interstitial inflammatory cells and release of inflammatory mediators, activation and proliferation of fibroblasts, and deposition of excessive extracellular matrix (ECM). The aim of this study was to evaluate the effect of human umbilical cord-derived mesenchymal stem cell (hucMSC) conditioned medium (CM) on renal tubulointerstitial inflammation and fibrosis. METHODS: Renal interstitial fibrosis was prepared in vivo using the unilateral ureteral obstruction (UUO)...
January 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29327082/evaluation-of-committed-and-primitive-cord-blood-progenitors-after-expansion-on-adipose-stromal-cells
#2
E R Andreeva, I V Andrianova, A N Gornostaeva, B Sh Gogiya, L B Buravkova
Umbilical cord blood mononuclear fraction is a valuable source of hematopoietic stem and progenitor cells (CB HSPCs). The rarity of this population is a serious limitation of its application in cell therapy. Ex vivo expansion enables to significantly amplify the number of hematopoietic precursors of different commitment. Here, we expand CB MNCs in co-culture with human adipose tissue-derived stromal cells (ASCs) to enrich HSPCs and describe phenotypic features of newly formed hematopoietic populations. The CD34+-HSPCs demonstrated 6-fold enrichment with 9000 CFUs per 50 × 103 HSPCs on average...
January 11, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29324880/edag-promotes-the-expansion-and-survival-of-human-cd34-cells
#3
Ke Zhao, Wei-Wei Zheng, Xiao-Ming Dong, Rong-Hua Yin, Rui Gao, Xiu Li, Jin-Fang Liu, Yi-Qun Zhan, Miao Yu, Hui Chen, Chang-Hui Ge, Hong-Mei Ning, Xiao-Ming Yang, Chang-Yan Li
EDAG is multifunctional transcriptional regulator primarily expressed in the linloc-kit+Sca-1+ hematopoietic stem cells (HSC) and CD34+ progenitor cells. Previous studies indicate that EDAG is required for maintaining hematopoietic lineage commitment balance. Here using ex vivo culture and HSC transplantation models, we report that EDAG enhances the proliferative potential of human cord blood CD34+ cells, increases survival, prevents cell apoptosis and promotes their repopulating capacity. Moreover, EDAG overexpression induces rapid entry of CD34+ cells into the cell cycle...
2018: PloS One
https://www.readbyqxmd.com/read/29321825/umbilical-cord-blood-stem-cell-treatment-for-a-patient-with-psoriatic-arthritis
#4
Margaret Coutts, Rowena Soriano, Rajendran Naidoo, Habib Torfi
Clinical and laboratory results document psoriatic arthritis in a 56-year old patient. The symptoms did not resolve with standard treatments (nonsteroidal anti-inflammatory drugs, steroids and methotrexate). TNF-alpha inhibitors (certolizumab pegol and adalimumab) were added to the treatment regime, with some adverse effects. A trial of human umbilical cord stem cell therapy was then initiated. The stem cells were enriched and concentrated from whole cord blood, by removal of erythrocytes and centrifugation...
December 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/29321654/motivating-cord-blood-donation-with-information-and-behavioral-nudges
#5
Daniela Grieco, Nicola Lacetera, Mario Macis, Daniela Di Martino
Umbilical cord blood is a source of hematopoietic stem cells essential to treat life-threatening diseases, such as leukemia and lymphoma. However, only a very small percentage of parents donate upon delivery. The decision to donate the cord blood occurs at a very specific time and when parents likely experience emotional, informational, and decisional overloads; these features of cord blood donation make it different from other pro-social activities. In collaboration with an OB-GYN clinic in Milan, Italy, we conducted the first randomized controlled trial that applies tools from behavioral science to foster cord blood donation, and quantified the gains that informational and behavioral "nudges" can achieve...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321508/thrombospondin-1-secreted-by-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-rescues-neurons-from-synaptic-dysfunction-in-alzheimer-s-disease-model
#6
Dong Hyun Kim, Hoon Lim, Dahm Lee, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Su Oh, Hyun Ho Hwang, Hong Bae Jeon
Alzheimer's disease (AD) is an incurable neurodegenerative disease characterised clinically by learning and memory impairments. Amyloid beta (Aβ) peptide-induced synaptic dysfunction is a pathological process associated with early-stage AD. Here, we show that paracrine action of human umbilical cord blood-derived-mesenchymal stem cells (hUCB-MSCs) protects the hippocampus from synaptic-density loss in in vitro and in vivo AD models. To identify paracrine factors underlying this rescue effect, we analysed hUCB-MSCs' secretome co-cultured with Aβ42-treated mouse hippocampal neurons...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29308505/-effect-of-mir-21-in-promoting-angiogenesis-differentiation-of-umbilical-cord-blood-mesenchymal-stem-cells-in-vitro
#7
Zhong-Kui Tian, Lin Liu, Dong-Lei Chen, Pei Wang, Jing-Ming Zhang, Yi Tang, Chun-Bo Tang
PURPOSE: To investigate the effect of miR-21 on promoting vascular differentiation of umbilical cord blood derived mesenchymal stem cells (UCBMSCs) in vitro. METHODS: UCBMSCs (from Stem Cell Bank of Tongji University) were cultured; Lenti-LacZ-Luciferase and Lenti-miR-21-Luciferase Lentiviral vector were reconstructed; Real-time fluorescence quantification PCR and Western blot were performed to detect the expression of key angiogenic factors at 0, 1, 4, 7 and 14 d after target gene was transduced into UCBMSCs; UCBMSCs of the third generation were transduced with Lenti-LacZ-Luciferase and Lenti-miR-21- Luciferase; matrigel tube assay was performed to observe the tube-like structure under microscope...
October 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29296964/alternative-donors-for-allogeneic-hematopoietic-stem-cell-transplantation-in-poor-risk-aml-in-cr1
#8
Jurjen Versluis, Myriam Labopin, Annalisa Ruggeri, Gerard Socie, Depei Wu, Liisa Volin, Didier Blaise, Noel Milpied, Charles Craddock, Ibrahim Yakoub-Agha, Johan Maertens, Per Ljungman, Anne Huynh, Mauricette Michallet, Eric Deconinck, Patrice Chevallier, Jakob Passweg, Fabio Ciceri, Mohamad Mohty, Jan J Cornelissen, Arnon Nagler
Allogeneic hematopoietic stem cell transplantation (alloHSCT) remains the treatment of choice to consolidate remission in patients with poor-risk acute myeloid leukemia (AML). With increasing alternative donors available, the preferred donor or stem cell source is debated. We set out to study outcome in recipients of alloHSCT with poor-risk AML in first complete remission (CR1) by donor type. A total of 6545 adult patients with poor-risk AML in CR1 receiving an alloHSCT using matched related donor (MRD, n = 3511) or alternative donors, including 10/10 (n = 1959) or 9/10 matched unrelated donors (MUDs, n = 549), umbilical cord blood (UCB) grafts (n = 333), or haplo-identical (haplo) donors (n = 193) were compared...
February 28, 2017: Blood Advances
https://www.readbyqxmd.com/read/29296932/not-just-a-marker-cd34-on-human-hematopoietic-stem-progenitor-cells-dominates-vascular-selectin-binding-along-with-cd44
#9
Dina B AbuSamra, Fajr A Aleisa, Asma S Al-Amoodi, Heba M Jalal Ahmed, Chee Jia Chin, Ayman F Abuelela, Ptissam Bergam, Rachid Sougrat, Jasmeen S Merzaban
CD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their migration to specialized microvascular beds in the bone marrow that express vascular selectins (E- and P-selectin). Here, we show that HSPC-enriched fractions from human hematopoietic tissue expressing CD34 (CD34pos) bound selectins, whereas those lacking CD34 (CD34neg) did not...
December 26, 2017: Blood Advances
https://www.readbyqxmd.com/read/29296868/cord-blood-transplantation-recapitulates-fetal-ontogeny-with-a-distinct-molecular-signature-that-supports-cd4-t-cell-reconstitution
#10
Prashant Hiwarkar, Mike Hubank, Waseem Qasim, Robert Chiesa, Kimberly C Gilmour, Aurore Saudemont, Persis J Amrolia, Paul Veys
Omission of in vivo T-cell depletion promotes rapid, thymic-independent CD4+-biased T-cell recovery after cord blood transplant. This enhanced T-cell reconstitution differs from that seen after stem cell transplant from other stem cell sources, but the mechanism is not known. Here, we demonstrate that the transcription profile of naive CD4+ T cells from cord blood and that of lymphocytes reconstituting after cord blood transplantation is similar to the transcription profile of fetal CD4+ T cells. This profile is distinct to that of naive CD4+ T cells from peripheral blood and that of lymphocytes reconstituting after T-replete bone marrow transplantation...
November 14, 2017: Blood Advances
https://www.readbyqxmd.com/read/29296828/gene-editing-rescue-of-a-novel-mpl-mutant-associated-with-congenital-amegakaryocytic-thrombocytopenia
#11
Cédric Cleyrat, Romain Girard, Eun H Choi, Éric Jeziorski, Thierry Lavabre-Bertrand, Sylvie Hermouet, Serge Carillo, Bridget S Wilson
Thrombopoietin (Tpo) and its receptor (Mpl) are the principal regulators of early and late thrombopoiesis and hematopoietic stem cell maintenance. Mutations in MPL can drastically impair its function and be a contributing factor in multiple hematologic malignancies, including congenital amegakaryocytic thrombocytopenia (CAMT). CAMT is characterized by severe thrombocytopenia at birth, which progresses to bone marrow failure and pancytopenia. Here we report unique familial cases of CAMT that presented with a previously unreported MPL mutation: T814C (W272R) in the background of the activating MPL G117T (K39N or Baltimore) mutation...
September 26, 2017: Blood Advances
https://www.readbyqxmd.com/read/29296747/single-umbilical-cord-blood-with-or-without-cd34-cells-from-a-third-party-donor-in-adults-with-leukemia
#12
Jaime Sanz, Mi Kwon, Guiomar Bautista, Miguel A Sanz, Pascual Balsalobre, José Luis Piñana, Carlos Solano, Rafael Duarte, Christelle Ferrá, Ignacio Lorenzo, Carmen Martín, Pere Barba, María Jesús Pascual, Rodrigo Martino, Jorge Gayoso, Ismael Buño, Carmen Regidor, Almudena de la Iglesia, Juan Montoro, José Luis Díez-Martín, Guillermo F Sanz, Rafael Cabrera
We retrospectively compared the clinical outcomes of adults with acute leukemia who received single-unit umbilical cord blood (UCB) transplantation (sUCBT) (n = 135) or stem cell transplant using coinfusion of a UCB graft with CD34+ cells from a third-party donor (Haplo-Cord) (n = 72) at different institutions within the Grupo Español de Trasplante Hematopoyético. In multivariable analysis, patients in the Haplo-Cord group showed more rapid neutrophil (hazard ratio [HR], 2.3; 95% confidence interval [CI], 1...
June 27, 2017: Blood Advances
https://www.readbyqxmd.com/read/29289934/wait-a-minute-an-observational-cohort-study-comparing-iron-stores-in-healthy-swedish-infants-at-4-months-of-age-after-10-60-and-180-second-umbilical-cord-clamping
#13
Ulrica Askelöf, Ola Andersson, Magnus Domellöf, Anders Fasth, Boubou Hallberg, Lena Hellström-Westas, Karin Pettersson, Magnus Westgren, Ingela E Wiklund, Cecilia Götherström
BACKGROUND AND OBJECTIVE: Umbilical cord blood (UCB) is a valuable stem cell source used for transplantation. Immediate umbilical cord (UC) clamping is widely practised, but delayed UC clamping is increasingly advocated to reduce possible infant anaemia. The aim of this study was to investigate an intermediate UC clamping time point and to evaluate iron status at the age of 4 months in infants who had the UC clamped after 60 s and compare the results with immediate and late UC clamping...
December 29, 2017: BMJ Open
https://www.readbyqxmd.com/read/29288821/reduced-intensity-allogeneic-transplant-for-acute-myeloid-leukemia-and-myelodysplastic-syndrome-using-combined-cd34-selected-haploidentical-graft-and-a-single-umbilical-cord-unit-compared-to-matched-unrelated-donor-stem-cells-in-older-adults
#14
Stephanie B Tsai, Joanna Rhodes, Hongtao Liu, Tsiporah Shore, Michael Bishop, Melissa M Cushing, Usama Gergis, Lucy Godley, Justin Kline, Richard A Larson, Sebastian Mayer, Olatoyosi Odenike, Wendy Stock, Amittha Wickrema, Koen van Besien, Andrew S Artz
Haplo/cord transplantation combines an umbilical cord blood (UCB) graft with CD34-selected haploidentical cells and results in rapid hematopoietic recovery followed by durable UCB engraftment. We compared outcomes of transplants in older patients with acute myeloid leukemia (AML) or high-risk myelodysplastic syndromes (MDS) who received either HLA-matched unrelated donor cells (MUD) or haplo/cord grafts. Between 2007 and 2013, 109 adults ages 50 and older underwent similar reduced intensity conditioning (RIC) with fludarabine and melphalan and antibody-mediated T-cell depletion for AML (n=83) or high risk MDS (n=26) followed by either a MUD (n=68) or haplo/cord (n=41) graft...
December 27, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29288817/low-body-mass-index-is-associated-with-increased-risk-of-acute-gvhd-after-umbilical-cord-blood-transplantation-in-children-and-young-adults-with-acute-leukemia-a-study-on-behalf-of-eurocord-and-the-ebmt-pediatric-disease-working-party
#15
Annalisa Paviglianiti, Jean Hugues Dalle, Mouhab Ayas, Jan Jaap Boelens, Fernanda Volt, Anna Paola Iori, Mair Pedro de Souza, Miguel Angel Diaz, Gerard Michel, Franco Locatelli, Charlotte Jubert, Ibrahim Yakoub-Agha, Henrique Bittencourt, Yves Bertrand, Chantal Kenzey, Karina Tozatto Maio, Hiromi Hayashi, Vanderson Rocha, Peter Bader, Eliane Gluckman, Annalisa Ruggeri
Body mass index (BMI) might influence outcomes after allogeneic stem cell transplantation (HSCT). However, the impact of BMI on survival in children undergoing HSCT is not well defined, with conflicting results being reported on this issue. We analyzed 855 patients aged 2 to 20 years with diagnosis of acute leukemia who underwent umbilical cord blood transplantation (UCBT) from 1990 to 2015. Patients were classified according to BMI as normal (5th-85th percentile), underweight (<5th percentile), overweight (85th-95th percentile) and obese (>95th percentile) using growth charts for age and gender...
December 27, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29285130/effects-of-hucb-mscs-on-recovery-of-neurological-function-and-tert-expression-in-brain-tissue-of-rats-with-cerebral-ischemia-reperfusion-injury
#16
Xiaohui Huang, Shuangli Zhang, Fuchun Li, Yuyun Zhou, Xiaohe Wang, Guojiao Fu, Xueling Ma
The aim of this study was to investigate and analyze the effects of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) on the recovery of neurological function and telomerase reverse transcriptase (TERT) expression in brain tissue of rats with cerebral ischemia-reperfusion injury. A total of 100 healthy adult Wistar rats were randomly divided into two groups: The control group and the observation group according to the random number table method. After the model of cerebral ischemia-reperfusion injury was established, the rats in the observation group were treated with hUCB-MSCs (10 ml/kg), while the rats in the control group were treated with saline every day...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29285089/malp-2-an-agonist-of-tlr6-promotes-the-immune-status-without-affecting-the-differentiation-capacity-of-umbilical-cord-mesenchymal-stem-cells
#17
Xiuli Wu, Lian Xu, Yangmei Shen, Na Yu, Yan Zhang, Tao Guo
Mesenchymal stem cells (MSCs) are increasingly used in cell-based therapy due to their multiple differentiation capacity, low expression of co-stimulatory factors and immunosuppressive effect. However, accumulating studies reported the recognition and rejection of engrafted MSCs, which eventually led to the fail of clinical trials. Toll-like receptors (TLRs) are important in mediating the immune response. In the present study, macrophage-activated lipopeptide-2 (MALP-2) was introduced to activate the TLR6 pathway in umbilical cord MSCs (UCMSCs)...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29283908/have-haploidentical-transplants-replaced-umbilical-cord-transplants-for-acute-leukemias
#18
Jia Hau Liu, Christopher G Kanakry, Leo Luznik
PURPOSE OF REVIEW: Haploidentical stem cell transplantation (Haplo SCT) and umbilical cord blood stem cell transplantation (UCB SCT) have emerged over the past two to three decades as viable sources of alternative donor SCT when a human leukocyte antigen matched donor is not available. However, which of these two donor types is optimal for patients with leukemia in need of allografting is unknown. RECENT FINDINGS: For patients with acute leukemia, results of UCB SCT have been improved by the use of double umbilical cord units and emerging ex vivo expansion technologies...
December 27, 2017: Current Opinion in Hematology
https://www.readbyqxmd.com/read/29277954/cryptosporidiosis-in-a-transplant-recipient-with-severe-intractable-diarrhea-detection-of-cryptosporidium-oocysts-by-intestinal-biopsies
#19
Kumiko Kagawa, Hikaru Fujino, Hirokazu Miki, Kimiko Sogabe, Mamiko Takahashi, Tomoko Maruhashi, Kengo Udaka, Masami Iwasa, Shiro Fujii, Shingen Nakamura, Masahiro Abe
Disseminated Cryptosporidium infection results in manifestations similar to those of graft versus host disease (GVHD), which hampers the detection of Cryptosporidium infection after allogeneic hematopoietic stem cell transplantation. Surveillance of oocysts on the surface of intestinal epithelial cells is needed for early and appropriate detection of Cryptosporidium infection in transplant recipients on immunosuppressants with severe intractable diarrhea. We present the first case of Cryptosporidium meleagridis infection in Japan after allogeneic cord blood transplantation...
December 26, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29277913/unrelated-donor-choices-for-allogeneic-hematopoietic-cell-transplantation-in-canada-an-evaluation-of-factors-influencing-donor-selection
#20
Valerie Greco-Stewart, Jeffrey Kiernan, Donna Killeen, Sherry Haun, Dena Mercer, Kimberly Young, Robert S Liwski, David S Allan
BACKGROUND: Utilization of unrelated donors and cord blood units (CBUs) for allogeneic hematopoietic cell transplantation continues to increase. Understanding the practices of donor selection by transplant centers is critical for unrelated donor registries and cord blood banks to optimize registry composition and inventory to meet patient need. STUDY DESIGN AND METHODS: Unrelated donor and CBU selection practices of Canadian transplant centers served by Canadian Blood Services' OneMatch Stem Cell & Marrow Network (OM) were reviewed, including HLA match level, locus of disparity, age, sex, and product choice (donor vs...
December 25, 2017: Transfusion
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