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https://www.readbyqxmd.com/read/28330281/evaluation-of-bioactive-secondary-metabolites-from-endophytic-fungus-pestalotiopsis-neglecta-bab-5510-isolated-from-leaves-of-cupressus-torulosa-d-don
#1
Deeksha Sharma, Avijit Pramanik, Pavan Kumar Agrawal
Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28302273/there-is-good-agreement-between-mr-enterography-and-bowel-ultrasound-with-regards-to-disease-location-and-activity-in-paediatric-inflammatory-bowel-disease
#2
J L Barber, J Maclachlan, K Planche, M Furman, D Crespi, N Bab, I Beal
AIM: To investigate concordance of bowel ultrasound and magnetic resonance enterography (MRE) in identifying active disease in children with inflammatory bowel disease. MATERIALS AND METHODS: The imaging of children with inflammatory bowel disease who had undergone bowel ultrasound and MRE within 30 days were retrospectively reviewed, from January 2009 to November 2015. Ultrasound was without oral contrast medium; MRI was conducted with patients unsedated with oral contrast medium and gadolinium...
March 14, 2017: Clinical Radiology
https://www.readbyqxmd.com/read/28272215/no-association-between-metformin-use-and-survival-in-patients-with-pancreatic-cancer-an-observational-cohort-study
#3
Martine A Frouws, Babs G Sibinga Mulder, Esther Bastiaannet, Marjolein M J Zanders, Myrthe P P van Herk-Sukel, Eleonora M de Leede, Bert A Bonsing, J Sven D Mieog, Cornelis J H Van de Velde, Gerrit-Jan Liefers
Several studies have suggested an association between use of metformin and an increased overall survival in patients diagnosed with pancreatic cancer, however with several important methodological limitations. The aim of the study was to assess the association between overall survival, pancreatic cancer, and metformin use.A retrospective cohort study of 1111 patients with pancreatic cancer was conducted using data from The Netherlands Comprehensive Cancer Organization (1998-2011). Data were linked to the PHARMO Database Network containing drug-dispensing records from community pharmacies...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28266709/bone-loss-in-c57bl-6j-olahsd-mice-a-substrain-of-c57bl-6j-carrying-mutated-alpha-synuclein-and-multimerin-1-genes
#4
Tamar Liron, Bitya Raphael, Sahar Hiram-Bab, Itai A Bab, Yankel Gabet
The inbred mouse strain C57BL/6 is commonly used for the generation of transgenic mouse and is a well-established strain in bone research. Different vendors supply different substrains of C57BL/6J as wild-type animals when genetic drift did not incur any noticeable phenotype. However, we sporadically observed drastic differences in the bone phenotype of 'WT' C57BL/6J mice originating from different labs and speculated that these variations are attributable, at least in part, to the variation between C57BL/6J substrains, which is often overlooked...
March 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28260453/first-report-on-stem-cell-transplant-from-iraq
#5
Abdul Majeed A Hammadi, Waleed A Azeez, Firas Hashim Jasim, Nadher Alshammary, Adel D Sewan, Khudhair Alrawaq, Ala Makki, Ahmed Mubarak, Amel Ameen, Andolina Marino, Ahmed Khudhair, Nedhal K Alrahhal
The Iraqi Bone Marrow Transplantation Center is located in the medical city complex of Bab Almuadham in Baghdad, Iraq. It was established on March 11, 2002, and performed its first mini-allotransplant for acute myeloid leukemia on January 24, 2003. Among 16 patients who received hematopoietic stem cell transplant between January 2003 and January 2010, one patient underwent allogeneic bone marrow transplant for acute myeloid leukemia and 15 patients received autologous bone marrow transplant for the following indications: 5 had multiple myeloma, 9 had lymphoma (8 with Hodgkin disease and 1 with non- Hodgkin lymphoma), and 1 had rhabdomyosarcoma...
February 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28256555/selection-characterization-and-application-of-artificial-dna-aptamer-containing-appended-bases-with-sub-nanomolar-affinity-for-a-salivary-biomarker
#6
Hirotaka Minagawa, Kentaro Onodera, Hiroto Fujita, Taiichi Sakamoto, Joe Akitomi, Naoto Kaneko, Ikuo Shiratori, Masayasu Kuwahara, Katsunori Horii, Iwao Waga
We have attained a chemically modified DNA aptamer against salivary α-amylase (sAA), which attracts researchers' attention as a useful biomarker for assessing human psychobiological and social behavioural processes, although high affinity aptamers have not been isolated from a random natural DNA library to date. For the selection, we used the base-appended base (BAB) modification, that is, a modified-base DNA library containing (E)-5-(2-(N-(2-(N6-adeninyl)ethyl))carbamylvinyl)-uracil in place of thymine. After eight rounds of selection, a 75 mer aptamer, AMYm1, which binds to sAA with extremely high affinity (Kd < 1 nM), was isolated...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28180936/fn14-hepatic-progenitor-cells-are-associated-with-liver-fibrosis-in-biliary-atresia
#7
Lulu Zheng, Zhibao Lv, Zhenhua Gong, Qingfeng Sheng, Zhimei Gao, Yuting Zhang, Shenghua Yu, Junmei Zhou, Zhengjun Xi, Xueli Wang
PURPOSE: The liver in biliary atresia (BA) is characterized by progressing fibrosis which is promoted by unclear reasons. We aimed to understand the factors influencing liver fibrosis. This study hypothesized that HPCs (hepatic progenitor cells) are activated and associated with liver fibrosis in biliary atresia. METHODS: Liver samples from biliary atresia patients are as BA group, and the normal liver derived from hepatoblastoma infants during operation are control group...
February 8, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28157424/a-rho-associated-kinase-inhibitor-protects-permeability-in-a-cell-culture-model-of-ocular-disease-and-reduces-aqueous-flare-in-anterior-uveitis
#8
Hiroshi Yamada, Masahiko Yoneda, Shingo Inaguma, Masahiko Gosho, Yusuke Murasawa, Zenzo Isogai, Masahiro Zako
PURPOSE: Recent clinical and experimental studies have reported favorable results when using Rho-associated kinase (ROCK) inhibitors for ocular disease, and in cell culture. Disruption of the human, nonpigmented ciliary epithelial cells (HNPCECs) that comprise the blood-aqueous barrier (BAB) induces anterior uveitis; these cells therefore provide a useful cell model of ocular disease. In this study, we examined the effects of ROCK inhibitors in anterior uveitis and in HNPCECs. METHODS: Aqueous flare values and intraocular pressures (IOPs) were determined in patients with anterior uveitis, 2 weeks after administration of ripasudil hydrochloride hydrate, a commercial ROCK inhibitor used to treat glaucoma or ocular hypertension...
February 3, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28130237/identification-of-protein-complexes-by-integrating-multiple-alignment-of-protein-interaction-networks
#9
Cheng-Yu Ma, Yi-Ping Phoebe Chen, Bonnie Berger, Chung-Shou Liao
MOTIVATION: Protein complexes are one of the keys to studying the behavior of a cell system. Many biological functions are carried out by protein complexes. During the past decade, the main strategy used to identify protein complexes from high-throughput network data has been to extract near-cliques or highly dense subgraphs from a single protein-protein interaction (PPI) network. Although experimental PPI data has increased significantly over recent years, most PPI networks still have many false positive interactions and false negative edge loss due to the limitations of high-throughput experiments...
January 27, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28079964/bab-random-c-monomer-sequence-via-radical-terpolymerization-of-limonene%C3%A2-a-maleimide%C3%A2-b-and-methacrylate%C3%A2-c-terpene-polymers-with-randomly-distributed-periodic-sequences
#10
Masataka Ojika, Kotaro Satoh, Masami Kamigaito
A naturally abundant terpene, limonene (A), was radically polymerized with a maleimide derivative (B) and methacrylate (C) in a fluorinated alcohol to give terpolymers with unprecedented BAB-random-C sequences in which the BAB monomer sequence was randomly copolymerized with a C unit. In each binary system, limonene was hardly copolymerized with methacrylate while it was efficiently copolymerized with maleimide to result in a 1:2-alternating BAB periodic sequence, in part due to the penultimate effects and hydrogen-bonding interactions with fluoroalcohol...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28006006/the-gulf-of-aden-intermediate-water-intrusion-regulates-the-southern-red-sea-summer-phytoplankton-blooms
#11
Denis Dreano, Dionysios E Raitsos, John Gittings, George Krokos, Ibrahim Hoteit
Knowledge on large-scale biological processes in the southern Red Sea is relatively limited, primarily due to the scarce in situ, and satellite-derived chlorophyll-a (Chl-a) datasets. During summer, adverse atmospheric conditions in the southern Red Sea (haze and clouds) have long severely limited the retrieval of satellite ocean colour observations. Recently, a new merged ocean colour product developed by the European Space Agency (ESA)-the Ocean Color Climate Change Initiative (OC-CCI)-has substantially improved the southern Red Sea coverage of Chl-a, allowing the discovery of unexpected intense summer blooms...
2016: PloS One
https://www.readbyqxmd.com/read/28000527/epigenetic-regulation-of-immune-checkpoints-another-target-for-cancer-immunotherapy
#12
Mahmoud A Ali, Marwa Matboli, Marwa Tarek, Maged Reda, Kamal M Kamal, Mahmoud Nouh, Ahmed M Ashry, Ahmed Fath El-Bab, Hend A Mesalam, Ayman El-Sayed Shafei, Omar Abdel-Rahman
Epigenetic changes in oncogenes and tumor-suppressor genes contribute to carcinogenesis. Understanding the epigenetic and genetic components of tumor immune evasion is crucial. Few cancer genetic mutations have been linked to direct correlations with immune evasion. Studies on the epigenetic modulation of the immune checkpoints have revealed a critical interaction between epigenetic and immune modulation. Epigenetic modifiers can activate many silenced genes. Some of them are immune checkpoints regulators that turn on immune responses and others turn them off resulting in immune evasion...
January 2017: Immunotherapy
https://www.readbyqxmd.com/read/27988311/first-detection-and-molecular-identification-of-babesia-microti-in-rattus-losea-captured-from-the-offshore-kinmen-island-of-taiwan
#13
Li-Lian Chao, Wen-Ching Yu, Chien-Ming Shih
Babesia microti was firstly detected and identified in brown country rats (Rattus losea, Swinhoe) captured from the offshore Kinmen Island of Taiwan. The prevalence of Babesia infection in 283 rodents was screened by polymerase chain reaction (PCR) assay using a piroplasma-conserved primer set (Piro A/B) and the thirty-seven PCR-positive rodents were further examined by PCR using a species-specific primer set (Bab 1/4) targeting the gene encoding the nuclear small-subunit ribosomal RNA (18S rRNA) of Babesia species...
December 12, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27959622/dwi-for-assessment-of-rectal-cancer-nodes-after-chemoradiotherapy-is-the-absence-of-nodes-at-dwi-proof-of-a-negative-nodal-status
#14
Miriam M van Heeswijk, Doenja M J Lambregts, Walter M Palm, Babs M F Hendriks, Monique Maas, Geerard L Beets, Regina G H Beets-Tan
OBJECTIVE: When considering organ preservation in patients with rectal cancer with good tumor response, assessment of a node-negative status after chemoradiation therapy (CRT) is important. DWI is a very sensitive technique to detect nodes. The study aim was to test the hypothesis that the absence of nodes at DWI after CRT is concordant with a ypN0 status. MATERIALS AND METHODS: A retrospective study was performed of 90 patients with rectal cancer treated with CRT followed by restaging MRI at 1...
March 2017: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/27942244/characterization-of-tamyb10-allelic-variants-and-development-of-sts-marker-for-pre-harvest-sprouting-resistance-in-chinese-bread-wheat
#15
Y Wang, X L Wang, J Y Meng, Y J Zhang, Z H He, Y Yang
Wheat grain color does not only affect the brightness of flour but also seed dormancy and pre-harvest sprouting (PHS) tolerance. The transcription factor Tamyb10 is an important candidate for R-1 gene, and the expression of its homologs determines wheat seed coat color. In the present study, the allelic variations of Tamyb10 were explored in a set of Chinese bread wheat varieties and advanced lines with different PHS tolerance, and a sequenced-tagged site (STS) marker for Tamyb10-D1 gene was developed, designated as Tamyb10D, which could be used as an efficient and reliable marker to evaluate the depth dormancy of wheat seeds...
2016: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/27899126/new-epidemiological-aspects-of-visceral-and-cutaneous-leishmaniasis-in-taza-morocco
#16
Maryam Hakkour, Asmae Hmamouch, Mohamed Mahmoud El Alem, Abdelkbir Rhalem, Fatima Amarir, Mohamed Touzani, Abderrahim Sadak, Hajiba Fellah, Faiza Sebti
BACKGROUND: Leishmaniasis is considered among the main endemic diseases in Morocco. However, further knowledge about epidemiological aspects of this disease is needed in several provinces to plan control and preventive strategies to tackle the disease. The present study aims to determine the epidemiological aspect of cutaneous and visceral leishmaniasis in Taza Province from 2007-2014 and to identify the circulating species in this province. RESULTS: The temporal study from 2007 to 2014 showed that the number of cutaneous leishmaniasis cases increased since 2010...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27888458/helicobacter-pylori-outer-membrane-protein-homc-shows-geographic-dependent-polymorphism-that-is-influenced-by-the-bab-family
#17
Aeryun Kim, Stephanie L Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong-Eui Jeon, Youngmin A Hong, Yun-Jung Yoo, D Scott Merrell, Jeong-Heon Cha
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27888376/laser-flare-cell-photometer-principle-and-significance-in-clinical-and-basic-ophthalmology
#18
REVIEW
Mitsuru Sawa
A slit-lamp examination is an indispensable and essential clinical evaluation method in ophthalmology, but, it is qualitative subjective. To complement its weaknesses in making a quantitative evaluation of flare intensity and number of cells in the aqueous humor in the eye, we invented the laser flare-cell photometer in 1988. The instrument enables a non-invasive quantitative evaluation of flare intensity and number of cells in the aqueous with good accuracy and repeatability as well as maneuverability equal to slit-lamp microscopy...
January 2017: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27885593/campylobacter-jejuni-isolation-enumeration-from-environmental-samples
#19
Kelli L Hiett
Currently, there is no universally accepted standard media or method for the recovery of Campylobacter species. This is likely due to the ubiquity of the organism in nature, the complex sample matrices from which the organism is often recovered, as well as the fragile/viable-but nonculturable state the organism assumes in response to stress. The use of a sterile filter placed upon a nonselective Brucella Agar Blood Plate (BAB), followed by incubation at 37 °C in a hydrogen-containing atmosphere (Campycheck), is one method to recover stressed and emerging Campylobacter spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27828855/the-cross-reactivity-of-binding-antibodies-with-different-interferon-beta-formulations-used-as-disease-modifying-drugs-in-multiple-sclerosis-patients
#20
Agnieszka Wencel-Warot, Slawomir Michalak, Marcin Warot, Alicja Kalinowska-Lyszczarz, Radoslaw Kazmierski
Interferon beta (IFNb) preparations are commonly used as first-line therapy in relapsing-remitting multiple sclerosis (RRMS). They are, however, characterized by limited efficacy, partly due to the formation of anti-IFNb antibodies in patients.In this pilot study, we assessed with the ELISA method the presence of the binding antibodies (BAbs) against interferon beta after 2 years of therapy with subcutaneous interferon beta 1a (Rebif) in 49 RRMS patients. Antibody levels were established again within 1 year after treatment withdrawal...
November 2016: Medicine (Baltimore)
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