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https://www.readbyqxmd.com/read/27899126/new-epidemiological-aspects-of-visceral-and-cutaneous-leishmaniasis-in-taza-morocco
#1
Maryam Hakkour, Asmae Hmamouch, Mohamed Mahmoud El Alem, Abdelkbir Rhalem, Fatima Amarir, Mohamed Touzani, Abderrahim Sadak, Hajiba Fellah, Faiza Sebti
BACKGROUND: Leishmaniasis is considered among the main endemic diseases in Morocco. However, further knowledge about epidemiological aspects of this disease is needed in several provinces to plan control and preventive strategies to tackle the disease. The present study aims to determine the epidemiological aspect of cutaneous and visceral leishmaniasis in Taza Province from 2007-2014 and to identify the circulating species in this province. RESULTS: The temporal study from 2007 to 2014 showed that the number of cutaneous leishmaniasis cases increased since 2010...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27888458/helicobacter-pylori-outer-membrane-protein-homc-shows-geographic-dependent-polymorphism-that-is-influenced-by-the-bab-family
#2
Aeryun Kim, Stephanie L Servetas, Jieun Kang, Jinmoon Kim, Sungil Jang, Yun Hui Choi, Hanfu Su, Yeong-Eui Jeon, Youngmin A Hong, Yun-Jung Yoo, D Scott Merrell, Jeong-Heon Cha
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H...
December 2016: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/27888376/laser-flare-cell-photometer-principle-and-significance-in-clinical-and-basic-ophthalmology
#3
REVIEW
Mitsuru Sawa
A slit-lamp examination is an indispensable and essential clinical evaluation method in ophthalmology, but, it is qualitative subjective. To complement its weaknesses in making a quantitative evaluation of flare intensity and number of cells in the aqueous humor in the eye, we invented the laser flare-cell photometer in 1988. The instrument enables a non-invasive quantitative evaluation of flare intensity and number of cells in the aqueous with good accuracy and repeatability as well as maneuverability equal to slit-lamp microscopy...
November 25, 2016: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27885593/campylobacter-jejuni-isolation-enumeration-from-environmental-samples
#4
Kelli L Hiett
Currently, there is no universally accepted standard media or method for the recovery of Campylobacter species. This is likely due to the ubiquity of the organism in nature, the complex sample matrices from which the organism is often recovered, as well as the fragile/viable-but nonculturable state the organism assumes in response to stress. The use of a sterile filter placed upon a nonselective Brucella Agar Blood Plate (BAB), followed by incubation at 37 °C in a hydrogen-containing atmosphere (Campycheck), is one method to recover stressed and emerging Campylobacter spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27828855/the-cross-reactivity-of-binding-antibodies-with-different-interferon-beta-formulations-used-as-disease-modifying-drugs-in-multiple-sclerosis-patients
#5
Agnieszka Wencel-Warot, Slawomir Michalak, Marcin Warot, Alicja Kalinowska-Lyszczarz, Radoslaw Kazmierski
Interferon beta (IFNb) preparations are commonly used as first-line therapy in relapsing-remitting multiple sclerosis (RRMS). They are, however, characterized by limited efficacy, partly due to the formation of anti-IFNb antibodies in patients.In this pilot study, we assessed with the ELISA method the presence of the binding antibodies (BAbs) against interferon beta after 2 years of therapy with subcutaneous interferon beta 1a (Rebif) in 49 RRMS patients. Antibody levels were established again within 1 year after treatment withdrawal...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27672215/targeted-next-generation-sequencing-of-fna-derived-dna-in-pancreatic-cancer
#6
Babs G Sibinga Mulder, J Sven D Mieog, Henricus J M Handgraaf, Arantza Farina Sarasqueta, Hans F A Vasen, Thomas P Potjer, Rutger-Jan Swijnenburg, Saskia A C Luelmo, Shirin Feshtali, Akin Inderson, Alexander L Vahrmeijer, Bert A Bonsing, Tom van Wezel, Hans Morreau
To improve the diagnostic value of fine-needle aspiration (FNA)-derived material, we perform targeted next-generation sequencing (NGS) in patients with a suspect lesion of the pancreas. The NGS analysis can lead to a change in the treatment plan or supports inconclusive or uncertain cytology results. We describe the advantages of NGS using one particular patient with a recurrent pancreatic lesion 7 years after resection of a pancreatic ductal adenocarcinoma (PDAC). Our NGS analysis revealed the presence of a presumed second primary cancer in the pancreatic remnant, which led to a change in treatment: resection with curative intend instead of palliation...
September 26, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27624940/selective-assembly-of-na-k-atpase-%C3%AE-2%C3%AE-2-heterodimers-in-the-heart-distinct-functional-properties-and-isoform-selective-inhibitors
#7
Michael Habeck, Elmira Tokhtaeva, Yotam Nadav, Efrat Ben Zeev, Sean P Ferris, Randal J Kaufman, Elizabeta Bab-Dinitz, Jack H Kaplan, Laura A Dada, Zvi Farfel, Daniel M Tal, Adriana Katz, George Sachs, Olga Vagin, Steven J D Karlish
The Na,K-ATPase α2 subunit plays a key role in cardiac muscle contraction by regulating intracellular Ca(2+), whereas α1 has a more conventional role of maintaining ion homeostasis. The β subunit differentially regulates maturation, trafficking, and activity of α-β heterodimers. It is not known whether the distinct role of α2 in the heart is related to selective assembly with a particular one of the three β isoforms. We show here by immunofluorescence and co-immunoprecipitation that α2 is preferentially expressed with β2 in T-tubules of cardiac myocytes, forming α2β2 heterodimers...
October 28, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27622276/retrospective-comparative-study-of-rigid-and-flexible-ureteroscopy-for-treatment-of-proximal-ureteral-stones
#8
Ehab Mohamad Galal, Ahmad Zaki Anwar, Tarek Khalaf Fath El-Bab, Amr Mohamad Abdelhamid
BACKGROUND: We analyzed the outcome and complications of rigid (R-URS) and flexible (F-URS) ureteroscopic lithotripsy for treatment of proximal ureteric stone (PUS). SUBJECTS AND METHODS: Retrospective data of 135 patients (93 males and 42 females) submitted to R-URS and F-URS for treatment of PUS in the period between July 2013 and January 2015 were investigated. (R-URS, group 1) was performed in 72 patients while 63 patients underwent (F-URS, group 2).We compared the 2 groups for success, stone characteristics, operative time, intraoperative and postoperative complications...
September 1, 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/27619080/homolytic-heterolytic-mesolytic-as-you-like-it-steering-the-cleavage-of-a-hc-sp-3-c-sp-3-h-bond-in-bis-1h-2-1-benzazaborole-derivatives
#9
Martin Hejda, Antonín Lyčka, Tomáš Mikysek, Roman Jambor, Aleš Růžička, Jaromír Vinklárek, Claudia Wilfer, Alexander Hoffmann, Sonja Herres-Pawlis, Libor Dostál
A set of (3,3')-bis(1-Ph-2-R-1H-2,1-benzazaborole) compounds, in which R=tBu (Bab-tBu)2 , R=Dipp (Bab-Dipp)2 or R=tBu and Dipp (Bab-Dipp)(Bab-tBu), was synthesized and fully characterized using (1) H, (11) B, (13) C, and (15) N NMR spectroscopy as well as single-crystal X-ray diffraction analysis. The central HC(sp(3) )-C(sp(3) )H bond with restricted rotation at the junction of both 1H-2,1-benzazaborole rings displayed an intriguing reactivity. It was demonstrated that this bond is easily mesolytically cleaved using alkali metals to form the respective aromatic 1Ph-2R-1H-2,1-benzazaborolyl anions M(+) (THF)n (Bab-tBu)(-) (M=Li, Na, K) and K(+) (THF)n (Bab-Dipp)(-) ...
October 17, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27604035/ablation-of-locally-advanced-pancreatic-cancer-with-percutaneous-irreversible-electroporation-results-of-the-phase-i-ii-panfire-study
#10
Hester J Scheffer, Laurien G P H Vroomen, Marcus C de Jong, Marleen C A M Melenhorst, Babs M Zonderhuis, Freek Daams, Jantien A Vogel, Marc G H Besselink, Cornelis van Kuijk, Jill Witvliet, Marian A E de van der Schueren, Tanja D de Gruijl, Anita G M Stam, Petrousjka M P van den Tol, Foke van Delft, Geert Kazemier, Martijn R Meijerink
Purpose To (a) investigate the safety of percutaneous irreversible electroporation (IRE) for locally advanced pancreatic cancer and (b) evaluate the quality of life (QOL), pain perception, and efficacy in terms of time to local progression, event-free survival, and overall survival (OS). Materials and Methods The study was approved by the local review board (NL42888.029.13). All patients provided written informed consent for study participation, the ablation procedure, and data usage. Between January 2014 and June 2015, 25 patients with histologically proved locally advanced pancreatic cancer 5 cm or smaller (13 women, 12 men; median age, 61 years; age range, 41-78 years) were prospectively included to undergo percutaneous computed tomographic-guided IRE...
September 6, 2016: Radiology
https://www.readbyqxmd.com/read/27591568/interaction-between-amphipathic-triblock-copolymers-and-l-%C3%AE-dipalmitoyl-phosphatidylcholine-large-unilamellar-vesicles
#11
M A Palominos, D Vilches, E Bossel, M A Soto-Arriaza
This study contributes to an understanding of how different polymeric structures, in special triblock copolymers can interact with the lipid bilayer. To study the phospholipid-copolymer vesicles system, we report the effect of two amphipathic triblock copolymers of the type BAB, i.e., hydrophobic-hydrophilic-hydrophobic triblock copolymers arranged as poly(ε-caprolactone)-poly(ethylene oxide)-poly(ε-caprolactone) (PCLn-PEOm-PCLn), where n=12 and m=45 for COP1 and n=16 and m=104 for COP2, on the dynamic and structural properties of dipalmitoyl-phosphatidylcholine (DPPC) large unilamellar vesicles (LUVs)...
August 27, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27587672/fast-genotyping-of-known-snps-through-approximate-k-mer-matching
#12
Ariya Shajii, Deniz Yorukoglu, Yun William Yu, Bonnie Berger
MOTIVATION: As the volume of next-generation sequencing (NGS) data increases, faster algorithms become necessary. Although speeding up individual components of a sequence analysis pipeline (e.g. read mapping) can reduce the computational cost of analysis, such approaches do not take full advantage of the particulars of a given problem. One problem of great interest, genotyping a known set of variants (e.g. dbSNP or Affymetrix SNPs), is important for characterization of known genetic traits and causative disease variants within an individual, as well as the initial stage of many ancestral and population genomic pipelines (e...
September 1, 2016: Bioinformatics
https://www.readbyqxmd.com/read/27566157/suitability-assessment-of-grey-water-quality-treated-with-an-upflow-downflow-siliceous-sand-marble-waste-filtration-system-for-agricultural-and-industrial-purposes
#13
Safa Chaabane, Khalifa Riahi, Hédi Hamrouni, Béchir Ben Thayer
The present study examines the suitability assessment of an upflow-downflow siliceous sand/marble waste filtration system for treatment and reuse of grey water collected from bathrooms of the student residential complex at the Higher Institute of Engineering Medjez El Bab (Tunisia). Once the optimization of grey water pre-treatment system has been determined, the filtration system was operated at different hydraulic loading rate and media filter proportions in order to assess the suitability of treated grey water for irrigational purpose according to salinity hazard, sodium hazard, magnesium hazard, permeability index, water infiltration rate, and widely used graphical methods...
August 26, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27561260/pyruvate-kinase-is-necessary-for-brucella-abortus-full-virulence-in-balb-c-mouse
#14
Jianpeng Gao, Mingxing Tian, Yanqing Bao, Peng Li, Jiameng Liu, Chan Ding, Shaohui Wang, Tao Li, Shengqing Yu
Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B...
2016: Veterinary Research
https://www.readbyqxmd.com/read/27539088/next-generation-multiresponsive-nanocarriers-for-targeted-drug-delivery-to-cancer-cells
#15
Peter T Altenbuchner, Patrick D L Werz, Patricia Schöppner, Friederike Adams, Alexander Kronast, Christina Schwarzenböck, Alexander Pöthig, Christian Jandl, Martin Haslbeck, Bernhard Rieger
C-H bond activation of 2-methoxyethylamino-bis(phenolate)-yttrium catalysts allowed the synthesis of BAB block copolymers comprised of 2-vinylpyridine (2VP; monomer A) and diethylvinylphosphonate (DEVP; monomer B) as the A and B blocks, respectively, by rare-earth-metal-mediated group-transfer polymerization (REM-GTP). The inherent multi-stimuli-responsive character and drug-loading and -release capabilities were observed to be dependent on the chain length and monomer ratios. Cytotoxicity assays revealed the biocompatibility and nontoxic nature of the obtained micelles toward ovarian cancer (HeLa) cells...
October 4, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27534537/role-of-microbial-translocation-in-soluble-cd14-up-regulation-in-hiv-but-not-in-hcv-infected-chimpanzees
#16
Babs E Verstrepen, Ivonne G Nieuwenhuis, Petra Mooij, Ernst J Verschoor, Zahra C Fagrouch, Ivanela Kondova, André Boonstra, Gerrit Koopman
During human immunodeficiency virus (HIV) infection, soluble CD14 (sCD14) is up-regulated as a consequence of pathological disruption of the gut epithelial barrier, and subsequent increased microbial translocation. Also in hepatitis C virus (HCV)-infected patients with advanced liver fibrosis, increased levels of sCD14 have been reported. Since the liver plays an important role in clearance of translocated bacterial products, hepatic fibrosis may negatively affect clearance and thus contribute to higher sCD14 levels...
October 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27493926/incidence-trends-of-colorectal-cancer-in-iran-during-2000-2009-a-population-based-study
#17
Edris Abdifard, Sudabe Amini, Sattar Bab, Nasim Masroor, Alice Khachian, Mohammad Heidari
BACKGROUND: As the third leading cause of death, cancers have a special place in the Iranian health system. Several studies have been done in some regions of the country with few years of follow-ups, but this was the first standardized study to investigate the incidence trends of colorectal cancer in Iran in a one-decade period. METHODS: The registered data for colorectal cancer cases in National Cancer Registry System were extracted from the Center for Disease Control & Management of Ministry of Health...
2016: Medical Journal of the Islamic Republic of Iran
https://www.readbyqxmd.com/read/27439541/the-efficacy-of-novel-anatomical-sites-for-the-assessment-of-muscle-oxygenation-during-central-hypovolemia
#18
Justin D Sprick, Babs R Soller, Caroline A Rickards
Muscle tissue oxygenation (SmO2) can track central blood volume loss associated with hemorrhage. Traditional peripheral measurement sites (e.g., forearm) may not be practical due to excessive movement or injury (e.g., amputation). The aim of this study was to evaluate the efficacy of three novel anatomical sites for the assessment of SmO2 under progressive central hypovolemia. 10 male volunteers were exposed to stepwise prone lower body negative pressure to decrease central blood volume, while SmO2 was assessed at four sites-the traditional site of the flexor carpi ulnaris (ARM), and three novel sites not previously investigated during lower body negative pressure, the deltoid, latissimus dorsi, and trapezius...
November 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27372472/disparities-in-revascularization-after-st-elevation-myocardial-infarction-stemi-before-and-after-the-2002-iom-report
#19
Oluwaseyi B Bolorunduro, Adekunle V Kiladejo, Islamiyat Babs Animashaun, Olakunle O Akinboboye
OBJECTIVES: To examine nationwide trends for racial disparities in Percutaneous Coronary Intervention after ST elevated Myocardial Infarction (STEMI). BACKGROUND: The Institute of Medicine (IOM) report published in 2002 showed that African Americans were less likely to receive coronary revascularization such as CABG and stents even after controlling for socioeconomics. It recommended increased awareness of these disparities among health professionals to reduce this...
May 2016: Journal of the National Medical Association
https://www.readbyqxmd.com/read/27366230/incidence-characterization-and-clinical-impact-analysis-of-peginterferon-beta1a-immunogenicity-in-patients-with-multiple-sclerosis-in-the-advance-trial
#20
Joleen T White, Scott D Newsome, Bernd C Kieseier, Robert A Bermel, Yue Cui, Ali Seddighzadeh, Serena Hung, Mary Crossman, Meena Subramanyam
BACKGROUND: Efficacy of interferon beta in multiple sclerosis (MS) can be dampened in patients who develop neutralizing antidrug antibodies (NAbs). Peginterferon beta1a is an interferon conjugated with a polyethylene glycol (PEG) moiety. Pegylation increases a drug's half life and exposure, and may also reduce immunogenicity. OBJECTIVE: The objective of this study was to characterize the incidence and impact of immunogenicity to peginterferon beta1a over 2 years in patients with MS...
July 2016: Therapeutic Advances in Neurological Disorders
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