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Marc Kirschner

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https://www.readbyqxmd.com/read/28723573/proteomic-and-metabolomic-characterization-of-a-mammalian-cellular-transition-from-quiescence-to-proliferation
#1
Ho-Joon Lee, Mark P Jedrychowski, Arunachalam Vinayagam, Ning Wu, Ng Shyh-Chang, Yanhui Hu, Chua Min-Wen, Jodene K Moore, John M Asara, Costas A Lyssiotis, Norbert Perrimon, Steven P Gygi, Lewis C Cantley, Marc W Kirschner
There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. The kinetics and regulation of these metabolic changes have not been properly evaluated. To correlate the orchestration of these processes with the cell cycle, we analyzed the transition from quiescence to proliferation of a non-malignant murine pro-B lymphocyte cell line in response to IL-3...
July 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28689658/conformational-landscape-of-the-p28-bound-human-proteasome-regulatory-particle
#2
Ying Lu, Jiayi Wu, Yuanchen Dong, Shuobing Chen, Shuangwu Sun, Yong-Bei Ma, Qi Ouyang, Daniel Finley, Marc W Kirschner, Youdong Mao
The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 Å resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions...
July 20, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28668406/vessel-shape-alterations-of-the-vertebrobasilar-arteries-in-mucopolysaccharidosis-type-iva-morquio-a-patients
#3
Yasemin Tanyildizi, Seyfullah Gökce, Federico Marini, Anna K Mayer, Stefanie Kirschner, Julia B Hennermann, Marc A Brockmann
PURPOSE: Main symptom of mucopolysaccharidosis type IVa (MPS IVa) is progressive systemic skeletal dysplasia. This is routinely monitored by cerebral and spinal MRI. The vascular system is generally not in the primary focus of interest. In our population of MPS IVa patients we observed vessel shape alterations of the vertebrobasilar arteries, which has not been described before. MATERIAL AND METHODS: MRI-datasets of 26 patients with MPS IVa acquired between 2008 and 2015 were eligible for retrospective analysis of the vertebrobasilar arteries...
August 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/28644940/percutaneous-treatment-of-unstable-scaphoid-waist-fractures
#4
Andrew P Matson, Ryan M Garcia, Marc J Richard, Fraser J Leversedge, J Mack Aldridge, David S Ruch
BACKGROUND: Percutaneous techniques have been described for the treatment of nondisplaced scaphoid fractures, although less information has been reported about outcomes for unstable, displaced fractures. The aim of this study was to evaluate the union and complication rates following manual closed reduction and percutaneous screw placement for a consecutive series of unstable, displaced scaphoid fractures. METHODS: A total of 28 patients (average age, 27 years; 22 male/6 female) were treated for isolated unstable displaced scaphoid waist fractures...
July 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28632594/outcome-of-early-and-late-diagnosed-essex-lopresti-injury
#5
Marc Schnetzke, Felix Porschke, Karin Hoppe, Stefan Studier-Fischer, Paul-Alfred Gruetzner, Thorsten Guehring
BACKGROUND: The aim of this study was to investigate the outcomes in a large series of Essex-Lopresti injuries (ELIs) and to compare patients with early and late diagnoses. METHODS: Thirty-one patients with an ELI (average age and standard deviation [SD], 46 ± 10 years) who were followed for a minimum of 2 years were included in the study. Patients were grouped according to whether the ELI had been diagnosed early (on the day of the injury) or late (>4 weeks after the radial head injury)...
June 21, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28416665/hippo-pathway-mediates-resistance-to-cytotoxic-drugs
#6
Taranjit S Gujral, Marc W Kirschner
Chemotherapy is widely used for cancer treatment, but its effectiveness is limited by drug resistance. Here, we report a mechanism by which cell density activates the Hippo pathway, which in turn inactivates YAP, leading to changes in the regulation of genes that control the intracellular concentrations of gemcitabine and several other US Food and Drug Administration (FDA)-approved oncology drugs. Hippo inactivation sensitizes a diverse panel of cell lines and human tumors to gemcitabine in 3D spheroid, mouse xenografts, and patient-derived xenograft models...
May 2, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28359158/relevant-insertion-site-anatomy-of-the-conventus-distal-radius-system
#7
Jacob Duncan, Marc Trzeciak
BACKGROUND: The Conventus Distal Radius System (DRS) is an intramedullary fixation scaffold inserted into the lateral aspect of the distal radius. The purpose of this study was to identify insertion site anatomy to illustrate risks associated with the minimally invasive nature of radial-sided implant application. METHODS: Ten cadavers were utilized. Using fluoroscopy, the 1.1-mm Kirschner wire and template was introduced per manufacturer's guidelines, access guide assembled, and dissection carried out to the superficial radial nerve (SRN) with preservation of the native location...
March 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28199398/a-segmentation-based-volumetric-approach-to-localize-and-quantify-cerebral-vasospasm-based-on-tomographic-imaging-data
#8
Axel Neulen, Tobias Pantel, Michael Kosterhon, Stefanie Kirschner, Marc A Brockmann, Sven R Kantelhardt, Alf Giese, Serge C Thal
INTRODUCTION: Quantification of cerebral vasospasm after subarachnoid hemorrhage (SAH) is crucial in animal studies as well as clinical routine. We have developed a method for computer-based volumetric assessment of intracranial blood vessels from cross-sectional imaging data. Here we demonstrate the quantification of vasospasm from micro computed tomography (micro-CT) data in a rodent SAH model and the transferability of the volumetric approach to clinical data. METHODS: We obtained rodent data by performing an ex vivo micro-CT of murine brains after sham surgery or SAH by endovascular filament perforation on day 3 post hemorrhage...
2017: PloS One
https://www.readbyqxmd.com/read/28174173/the-apc-c-e3-ligase-complex-activator-fzr1-restricts-braf-oncogenic-function
#9
Lixin Wan, Ming Chen, Juxiang Cao, Xiangpeng Dai, Qing Yin, Jinfang Zhang, Su-Jung Song, Ying Lu, Jing Liu, Hiroyuki Inuzuka, Jesse M Katon, Kelsey Berry, Jacqueline Fung, Christopher Ng, Pengda Liu, Min Sup Song, Lian Xue, Roderick T Bronson, Marc W Kirschner, Rutao Cui, Pier Paolo Pandolfi, Wenyi Wei
BRAF drives tumorigenesis by coordinating the activation of the RAS/RAF/MEK/ERK oncogenic signaling cascade. However, upstream pathways governing BRAF kinase activity and protein stability remain undefined. Here, we report that in primary cells with active APC(FZR1), APC(FZR1) earmarks BRAF for ubiquitination-mediated proteolysis, whereas in cancer cells with APC-free FZR1, FZR1 suppresses BRAF through disrupting BRAF dimerization. Moreover, we identified FZR1 as a direct target of ERK and CYCLIN D1/CDK4 kinases...
April 2017: Cancer Discovery
https://www.readbyqxmd.com/read/27829015/imaging-of-orthotopic-glioblastoma-xenografts-in-mice-using-a-clinical-ct-scanner-comparison-with-micro-ct-and-histology
#10
COMPARATIVE STUDY
Stefanie Kirschner, Bettina Mürle, Manuela Felix, Anna Arns, Christoph Groden, Frederik Wenz, Andreas Hug, Gerhard Glatting, Martin Kramer, Frank A Giordano, Marc A Brockmann
PURPOSE: There is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated. MATERIALS AND METHODS: 2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i...
2016: PloS One
https://www.readbyqxmd.com/read/27791164/structural-basis-for-dynamic-regulation-of-the-human-26s-proteasome
#11
Shuobing Chen, Jiayi Wu, Ying Lu, Yong-Bei Ma, Byung-Hoon Lee, Zhou Yu, Qi Ouyang, Daniel J Finley, Marc W Kirschner, Youdong Mao
The proteasome is the major engine of protein degradation in all eukaryotic cells. At the heart of this machine is a heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitylated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides. Using cryoelectron microscopy, we determined a near-atomic-resolution structure of the 2.5-MDa human proteasome in its ground state, as well as subnanometer-resolution structures of the holoenzyme in three alternative conformational states...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27275537/the-hiv-derived-protein-vpr52-96-has-anti-glioma-activity-in-vitro-and-in-vivo
#12
Jens Kübler, Stefanie Kirschner, Linda Hartmann, Grit Welzel, Maren Engelhardt, Carsten Herskind, Marlon R Veldwijk, Christian Schultz, Manuela Felix, Gerhard Glatting, Patrick Maier, Frederik Wenz, Marc A Brockmann, Frank A Giordano
Patients with actively replicating human immunodeficiency virus (HIV) exhibit adverse reactions even to low irradiation doses. High levels of the virus-encoded viral protein R (Vpr) are believed to be one of the major underlying causes for increased radiosensitivity. As Vpr efficiently crosses the blood-brain barrier and accumulates in astrocytes, we examined its efficacy as a drug for treatment of glioblastoma multiforme (GBM).In vitro, four glioblastoma-derived cell lines with and without methylguanine-DNA methyltransferase (MGMT) overexpression (U251, U87, U251-MGMT, U87-MGMT) were exposed to Vpr, temozolomide (TMZ), conventional photon irradiation (2 to 6 Gy) or to combinations thereof...
July 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/27259151/dual-ring-e3-architectures-regulate-multiubiquitination-and-ubiquitin-chain-elongation-by-apc-c
#13
Nicholas G Brown, Ryan VanderLinden, Edmond R Watson, Florian Weissmann, Alban Ordureau, Kuen-Phon Wu, Wei Zhang, Shanshan Yu, Peter Y Mercredi, Joseph S Harrison, Iain F Davidson, Renping Qiao, Ying Lu, Prakash Dube, Michael R Brunner, Christy R R Grace, Darcie J Miller, David Haselbach, Marc A Jarvis, Masaya Yamaguchi, David Yanishevski, Georg Petzold, Sachdev S Sidhu, Brian Kuhlman, Marc W Kirschner, J Wade Harper, Jan-Michael Peters, Holger Stark, Brenda A Schulman
Protein ubiquitination involves E1, E2, and E3 trienzyme cascades. E2 and RING E3 enzymes often collaborate to first prime a substrate with a single ubiquitin (UB) and then achieve different forms of polyubiquitination: multiubiquitination of several sites and elongation of linkage-specific UB chains. Here, cryo-EM and biochemistry show that the human E3 anaphase-promoting complex/cyclosome (APC/C) and its two partner E2s, UBE2C (aka UBCH10) and UBE2S, adopt specialized catalytic architectures for these two distinct forms of polyubiquitination...
June 2, 2016: Cell
https://www.readbyqxmd.com/read/27199406/scientific-community-preprints-for-the-life-sciences
#14
Jeremy M Berg, Needhi Bhalla, Philip E Bourne, Martin Chalfie, David G Drubin, James S Fraser, Carol W Greider, Michael Hendricks, Chonnettia Jones, Robert Kiley, Susan King, Marc W Kirschner, Harlan M Krumholz, Ruth Lehmann, Maria Leptin, Bernd Pulverer, Brooke Rosenzweig, John E Spiro, Michael Stebbins, Carly Strasser, Sowmya Swaminathan, Paul Turner, Ronald D Vale, K VijayRaghavan, Cynthia Wolberger
No abstract text is available yet for this article.
May 20, 2016: Science
https://www.readbyqxmd.com/read/27102219/the-gonium-pectorale-genome-demonstrates-co-option-of-cell-cycle-regulation-during-the-evolution-of-multicellularity
#15
Erik R Hanschen, Tara N Marriage, Patrick J Ferris, Takashi Hamaji, Atsushi Toyoda, Asao Fujiyama, Rafik Neme, Hideki Noguchi, Yohei Minakuchi, Masahiro Suzuki, Hiroko Kawai-Toyooka, David R Smith, Halle Sparks, Jaden Anderson, Robert Bakarić, Victor Luria, Amir Karger, Marc W Kirschner, Pierre M Durand, Richard E Michod, Hisayoshi Nozaki, Bradley J S C Olson
The transition to multicellularity has occurred numerous times in all domains of life, yet its initial steps are poorly understood. The volvocine green algae are a tractable system for understanding the genetic basis of multicellularity including the initial formation of cooperative cell groups. Here we report the genome sequence of the undifferentiated colonial alga, Gonium pectorale, where group formation evolved by co-option of the retinoblastoma cell cycle regulatory pathway. Significantly, expression of the Gonium retinoblastoma cell cycle regulator in unicellular Chlamydomonas causes it to become colonial...
2016: Nature Communications
https://www.readbyqxmd.com/read/26714185/correction-dorsoventral-patterning-in-hemichordates-insights-into-early-chordate-evolution
#16
Christopher J Lowe, Mark Terasaki, Michael Wu, Robert M Freeman, Linda Runft, Kristen Kwan, Saori Haigo, Jochanan Aronowicz, Eric Lander, Chris Gruber, Mark Smith, Marc Kirschner, John Gerhart
No abstract text is available yet for this article.
December 2015: PLoS Biology
https://www.readbyqxmd.com/read/26677176/systems-medicine-sketching-the-landscape
#17
REVIEW
Marc Kirschner
To understand the meaning of the term Systems Medicine and to distinguish it from seemingly related other expressions currently in use, such as precision, personalized, -omics, or big data medicine, its underlying history and development into present time needs to be highlighted. Having this development in mind, it becomes evident that Systems Medicine is a genuine concept as well as a novel way of tackling the manifold complexity that occurs in nowadays clinical medicine-and not just a rebranding of what has previously been done in the past...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26613472/separating-exchange-splitting-from-spin-mixing-in-gadolinium-by-femtosecond-laser-excitation
#18
Beatrice Andres, Marc Christ, Cornelius Gahl, Marko Wietstruk, Martin Weinelt, Jürgen Kirschner
Employing spin-, time-, and energy-resolved photoemission spectroscopy, we present the first study on the spin polarization of a single electronic state after ultrafast optical excitation. Our investigation concentrates on the majority-spin component of the d-band-derived Gd(0001) surface state d(z(2))(↑). While its binding energy shows a rapid Stoner-like shift by 90 meV with an exponential time constant of τ(E)=0.6±0.1 ps, the d(z(2))(↑) spin polarization remains nearly constant within the first picoseconds and decays with τ(S)=15±8  ps...
November 13, 2015: Physical Review Letters
https://www.readbyqxmd.com/read/26598613/resonant-microchannel-volume-and-mass-measurements-show-that-suspended-cells-swell-during-mitosis
#19
Sungmin Son, Joon Ho Kang, Seungeun Oh, Marc W Kirschner, T J Mitchison, Scott Manalis
Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro-B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0...
November 23, 2015: Journal of Cell Biology
https://www.readbyqxmd.com/read/26580012/hemichordate-genomes-and-deuterostome-origins
#20
COMPARATIVE STUDY
Oleg Simakov, Takeshi Kawashima, Ferdinand Marlétaz, Jerry Jenkins, Ryo Koyanagi, Therese Mitros, Kanako Hisata, Jessen Bredeson, Eiichi Shoguchi, Fuki Gyoja, Jia-Xing Yue, Yi-Chih Chen, Robert M Freeman, Akane Sasaki, Tomoe Hikosaka-Katayama, Atsuko Sato, Manabu Fujie, Kenneth W Baughman, Judith Levine, Paul Gonzalez, Christopher Cameron, Jens H Fritzenwanker, Ariel M Pani, Hiroki Goto, Miyuki Kanda, Nana Arakaki, Shinichi Yamasaki, Jiaxin Qu, Andrew Cree, Yan Ding, Huyen H Dinh, Shannon Dugan, Michael Holder, Shalini N Jhangiani, Christie L Kovar, Sandra L Lee, Lora R Lewis, Donna Morton, Lynne V Nazareth, Geoffrey Okwuonu, Jireh Santibanez, Rui Chen, Stephen Richards, Donna M Muzny, Andrew Gillis, Leonid Peshkin, Michael Wu, Tom Humphreys, Yi-Hsien Su, Nicholas H Putnam, Jeremy Schmutz, Asao Fujiyama, Jr-Kai Yu, Kunifumi Tagawa, Kim C Worley, Richard A Gibbs, Marc W Kirschner, Christopher J Lowe, Noriyuki Satoh, Daniel S Rokhsar, John Gerhart
Acorn worms, also known as enteropneust (literally, 'gut-breathing') hemichordates, are marine invertebrates that share features with echinoderms and chordates. Together, these three phyla comprise the deuterostomes. Here we report the draft genome sequences of two acorn worms, Saccoglossus kowalevskii and Ptychodera flava. By comparing them with diverse bilaterian genomes, we identify shared traits that were probably inherited from the last common deuterostome ancestor, and then explore evolutionary trajectories leading from this ancestor to hemichordates, echinoderms and chordates...
November 26, 2015: Nature
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