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https://www.readbyqxmd.com/read/28681900/tile-based-self-assembly-of-a-triple-helical-polysaccharide-into-cell-wall-like-mesoporous-nanocapsules
#1
Chaoxi Wu, Xiaoying Wang, Jianjing Wang, Zhen Zhang, Zhiping Wang, Yifei Wang, Shunqing Tang
Tile-based self-assembly is a robust system in the construction of three-dimensional DNA nanostructures but it has been rarely applied to other helical biopolymers. β-Glucan is an immunoactive natural polymer which exists in a triple helical conformation. Herein, we report that β-glucan, after modification using two types of short chain acyl groups, can self-assemble into tiles with inactivated sticky ends at the interface of two solvents. These tiles consist of a single layer of helices laterally aligned, and the sticky ends can be activated when a few acyl groups at the ends are removed; these tiles can further pack into mesoporous nanocapsules, in a similar process as the sticky DNA tiles pack into complex polyhedral nano-objects...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28654071/protocols-for-c-brick-dna-standard-assembly-using-cpf1
#2
Shi-Yuan Li, Guo-Ping Zhao, Jin Wang
CRISPR-associated protein Cpf1 cleaves double-stranded DNA under the guidance of CRISPR RNA (crRNA), generating sticky ends. Because of this characteristic, Cpf1 has been used for the establishment of a DNA assembly standard called C-Brick, which has the advantage of long recognition sites and short scars. On a standard C-Brick vector, there are four Cpf1 recognition sites - the prefix (T1 and T2 sites) and the suffix (T3 and T4 sites) - flanking biological DNA parts. The cleavage of T2 and T3 sites produces complementary sticky ends, which allow for the assembly of DNA parts with T2 and T3 sites...
June 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28652345/dna-cytoskeleton-for-stabilizing-artificial-cells
#3
Chikako Kurokawa, Kei Fujiwara, Masamune Morita, Ibuki Kawamata, Yui Kawagishi, Atsushi Sakai, Yoshihiro Murayama, Shin-Ichiro M Nomura, Satoshi Murata, Masahiro Takinoue, Miho Yanagisawa
Cell-sized liposomes and droplets coated with lipid layers have been used as platforms for understanding live cells, constructing artificial cells, and implementing functional biomedical tools such as biosensing platforms and drug delivery systems. However, these systems are very fragile, which results from the absence of cytoskeletons in these systems. Here, we construct an artificial cytoskeleton using DNA nanostructures. The designed DNA oligomers form a Y-shaped nanostructure and connect to each other with their complementary sticky ends to form networks...
June 26, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28590320/sticky-silicone-oil
#4
Hamouda Hamdi Ghoraba, Adel Galal Zaky, Haithem Mamoun Abd Al Fatah, Emad El Din Mohamed El Gemai, Mohamed Amin Heikal
OBJECTIVE: To report and analyze the phenomenon of sticky silicone oil on the retina at the time of its removal. BACKGROUND: Silicone oil has been used for more than 25 years as a long-term retinal tamponade in the surgical management of patients with complex retinal detachments. When a decision to remove silicone oil was made at the end of the tamponade period, the system involved a number of different materials: silicone oil, aqueous solution with or without remnants of perfluorocarbon liquid plus the material of the cannula used to aspirate Silicone oil...
August 2017: Retina
https://www.readbyqxmd.com/read/28573294/transition-from-nematic-to-gyroid-type-cubic-soft-self-assembly-by-side-chain-engineering-of-%C3%AF-conjugated-sticky-rods
#5
Marco Poppe, Changlong Chen, Helgard Ebert, Silvio Poppe, Marko Prehm, Christoph Kerzig, Feng Liu, Carsten Tschierske
A sequence of liquid crystalline phases, involving cybotactic nematics, a lamellar phase, bicontinuous cubics and triangular honeycombs, was observed for oligo(phenylene ethynylene) based X-shaped bolapolyphiles with two long lateral alkyl chains and sticky ends provided by glycerol groups. In the cubic phase with Ia3[combining macron]d lattice - which is tailored by alkyl chain engineering - the aromatic cores are organized on the gyroid minimal surface in 3D curved layers of almost parallel aligned π-conjugated rods...
June 21, 2017: Soft Matter
https://www.readbyqxmd.com/read/28514850/dna-dendrimer-streptavidin-nanocomplex-an-efficient-signal-amplifier-for-construction-of-biosensing-platforms
#6
Yan Zhao, Shichao Hu, Huaming Wang, Kaiwen Yu, Yan Guan, Xiaoyun Liu, Na Li, Feng Liu
We develop a DNA dendrimer-streptavidin (SA) nanocomplex as a novel signal amplifier to create biosensing platforms for disease-related species. The DNA dendrimer-SA nanocomplex is fabricated by cross-linking the nonlinear hybridization chain reaction based DNA dendrimer with the SA-coupled linker DNA and possesses multiple sticky ends, a high molecular weight, and a hyperbranched nanostructure with large numbers of DNA duplexes. Taking advantage of the DNA dendrimer-SA nanocomplex and a label-free quartz crystal microbalance (QCM) technology, we first construct a mass-sensitive QCM biosensing platform for nucleic acids, which displays high selectivity and sensitivity, with a detection limit of 0...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28504444/diversity-ecology-and-seasonality-of-sand-flies-diptera-psychodidae-of-the-jenin-district-palestinian-territories
#7
Samir S Sawalha, Asad Ramlawi, Ramzi M Sansur, Ibrahim Mohammad Salem, Zuhair S Amr
The diversity, ecology, and seasonality for sand flies from two localities in Jenin District, the Palestinian Territories, were studied. A total of 12,579 sand flies (5,420 Phlebotomus and 7,159 Sergentomyia) were collected during the study period. The genera Phlebotomus and Sergentomyia are represented by 13 and nine species and subspecies, respectively. Species account was given for all collected species. CDC light traps yielded 7,649 (60.8%) of the total captured sand flies, while sticky traps and aspirators contributed to 36...
June 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/28443918/ph-responsiveness-of-hydrogels-formed-by-telechelic-polyampholytes
#8
Margarita A Dyakonova, George Gotzamanis, Bart-Jan Niebuur, Natalya S Vishnevetskaya, Konstantinos N Raftopoulos, Zhenyu Di, Sergey K Filippov, Constantinos Tsitsilianis, Christine M Papadakis
We investigate the influence of pH on the rheological and structural properties of hydrogels formed by hydrophobic association of the sticky ends of the triblock terpolymer poly(methyl methacrylate)-b-poly(2-(diethylamino)ethyl methacrylate-co-methacrylic acid)-b-poly(methyl methacrylate) (PMMA-b-P(DEA-co-MAA)-b-PMMA). The middle block is a weak polyampholyte having a pH dependent charge density and sign, which enables tuning of the rheological and structural properties by pH variation. Small-angle neutron scattering (SANS) studies of solutions in D2O at 0...
April 26, 2017: Soft Matter
https://www.readbyqxmd.com/read/28334880/dna-nanostructures-constructed-with-multi-stranded-motifs
#9
Donglei Yang, Zhenyu Tan, Yongli Mi, Bryan Wei
Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The length of sticky ends can be adjusted to form different dihedral angles between the matching motifs, which corresponds to different connecting patterns. Moreover, the length of rigidity core can also be tuned to result in different dihedral angles between the component helices of a certain motif therefore different numbers of component helices...
April 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28211680/two-way-gold-nanoparticle-label-free-sensing-of-specific-sequence-and-small-molecule-targets-using-switchable-concatemers
#10
Longjiao Zhu, Xiangli Shao, Yunbo Luo, Kunlung Huang, Wentao Xu
A two-way colorimetric biosensor based on unmodified gold nanoparticles (GNPs) and a switchable double-stranded DNA (dsDNA) concatemer have been demonstrated. Two hairpin probes (H1 and H2) were first designed that provided the fuels to assemble the dsDNA concatemers via hybridization chain reaction (HCR). A functional hairpin (FH) was rationally designed to recognize the target sequences. All the hairpins contained a single-stranded DNA (ssDNA) loop and sticky end to prevent GNPs from salt-induced aggregation...
April 4, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28165224/programmable-dna-guided-artificial-restriction-enzymes
#11
Behnam Enghiad, Huimin Zhao
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA sequences at virtually any arbitrary site and generating defined sticky ends of varying length. Short DNA guides are used to direct PfAgo to target sites for cleavage at high temperatures (>87 °C) followed by reannealing of the cleaved single stranded DNAs...
February 6, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28115632/the-cctl-cpf1-assisted-cutting-and-taq-dna-ligase-assisted-ligation-method-for-efficient-editing-of-large-dna-constructs-in-vitro
#12
Chao Lei, Shi-Yuan Li, Jia-Kun Liu, Xuan Zheng, Guo-Ping Zhao, Jin Wang
As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly. Here, the Cpf1 cleavage sites were precisely characterized, where the cleavage site on the target strand was around the 22nd base relative to the protospacer adjacent motif site, but the cleavage on the non-target strand was affected by the spacer length. When the spacer length was 20 nt or longer, Cpf1 mainly cleaved around the 14th and the 18th bases on the non-target strand; otherwise, with a shorter spacer (i...
May 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28107938/nucleotide-base-analog-pyrrolo-deoxycytidine-as-fluorescent-probe-signal-for-enzyme-free-and-signal-amplified-nucleic-acids-detection
#13
Zhijuan Duan, Zhuo Li, Jianyuan Dai, Hongfei He, Dan Xiao
In the present work, an enzyme-free and signal amplified nucleic acids detection method based on the fluorescent nucleotide base analog pyrrolo-deoxycytidine (P-dC) and catalyzed hairpin assembly (CHA) has been developed. In the CHA signal amplification system, two hairpin auxiliary probes, H1 and H2, which containing a fluorescent P-dC at the end of the stem, respectively, were used as the fluorescent probes. The fluorescence of P-dC in H1 and H2 was quenched owing to the stacking interaction among the bases in the stem...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28032108/faunal-distribution-and-seasonal-bio-ecology-of-naturally-infected-sand-flies-in-a-new-endemic-zoonotic-cutaneous-leishmaniasis-focus-of-southern-iran
#14
Kourosh Azizi, Hayedeh Parvinjahromi, Mohammad Djaefar Moemenbellah-Fard, Bahador Sarkari, Mohammad Reza Fakoorziba
BACKGROUND: Cutaneous leishmaniasis is a major health problem in Iran in spite of implementation of control program. This infectious disease caused morbidity in less than 27000 people in 2010. This study was set to determine some ecological aspects of sand flies in Fasa district, Fars Province, southern Iran during 2011-2012. METHODS: A total of 4792 sand flies were captured by means of sticky paper and CDC miniature light traps in 10 selected villages from the beginning to the end of the active season, from which 1115 specimens were captured for abundance study and 3677 specimens captured for monitoring monthly activities in Fasa...
December 2016: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/27987754/incorporation-of-biguanide-compounds-into-poly-gl-b-poly-gl-co-tmc-co-cl-b-poly-gl-monofilament-surgical-sutures
#15
Yolanda Márquez, Tània Cabral, Alice Lorenzetti, Lourdes Franco, Pau Turon, Luís J Del Valle, Jordi Puiggalí
A new biodegradable coating was developed for bioabsorbable monofilament sutures. Specifically, a random copolymer having 35wt-% and 65wt-% of lactide and trimethylene carbonate units showed appropriate flexibility, stickiness and degradation rate, as well as capability to produce a complete and uniform coating. Monofilament sutures of polyglycolide-b-poly(glycolide-co-trimethylene carbonate-co-ε-caprolactone)-b-polyglycolide were loaded with chlorhexidine (CHX) and poly(hexamethylene biguanide) (PHMB) to explore the possibility to achieve antimicrobial activity without adverse cytotoxic effects...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27937049/agglomeration-of-celecoxib-by-quasi-emulsion-solvent-diffusion-method-without-stabilizer-effect-of-good-solvent
#16
Maryam Maghsoodi, Ali Nokhodchi
AIM: The aim of the present research is to investigate the feasibility of agglomeration of crystals by the quasi emulsion solvent diffusion method without using a stabilizer. METHOD: Two solvent systems comprising a solvent and an antisolvent (water) were used to prepare celecoxib agglomerates. To this end, seven solvents including propanol, methyl acetate, methyl ethyl ketone, butanol, ethyl acetate, isopropyl acetate and pentanol were examined. The agglomerates were evaluated by micromeritic properties (e...
December 12, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27929588/use-of-%C3%AE-cyclodextrin-tethered-cationic-polymer-based-fluorescence-enhancement-of-pyrene-and-hybridization-chain-reaction-for-the-enzyme-free-amplified-detection-of-dna
#17
Chunxia Song, Bingjie Li, Xiaohai Yang, Kemin Wang, Qing Wang, Jianbo Liu, Jin Huang
Herein, we proposed an enzyme-free strategy for the amplified detection of DNA by combining the efficient fluorescence enhancement capability of a β-cyclodextrin-tethered cationic polymer (cationic polyβ-CD) to pyrene with the amplification capability of target DNA triggered hybridization chain reaction (HCR). Cationic polyβ-CD with positive charge was synthesized. Two hairpin probes, H1 and H2, were employed in the system and the pyrene-labelled H2 was chosen as the signal unit. The pyrene attached on the sticky end of H2 was flexible and there was strong electrostatic interaction between cationic polyβ-CD and negatively-charged H2, so pyrene could easily enter the cavity of CD that is tethered on the cationic polymer, accompanied by significant fluorescence enhancement...
December 19, 2016: Analyst
https://www.readbyqxmd.com/read/27905529/efficient-targeted-mutagenesis-of-rice-and-tobacco-genomes-using-cpf1-from-francisella-novicida
#18
Akira Endo, Mikami Masafumi, Hidetaka Kaya, Seiichi Toki
CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared to Cas9. First, Cpf1 utilizes a thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers a guanidine-rich PAM. Cpf1 could be used as a sequence-specific nuclease to target AT-rich regions of a genome that Cas9 had difficulty accessing. Second, Cpf1 generates DNA ends with a 5' overhang, whereas Cas9 creates blunt DNA ends after cleavage...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27825528/hybridization-chain-reaction-based-colorimetric-aptasensor-of-adenosine-5-triphosphate-on-unmodified-gold-nanoparticles-and-two-label-free-hairpin-probes
#19
Zhuangqiang Gao, Zhenli Qiu, Minghua Lu, Jian Shu, Dianping Tang
This work designs a new label-free aptasensor for the colorimetric determination of small molecules (adenosine 5'-triphosphate, ATP) by using visible gold nanoparticles as the signal-generation tags, based on target-triggered hybridization chain reaction (HCR) between two hairpin DNA probes. The assay is carried out referring to the change in the color/absorbance by salt-induced aggregation of gold nanoparticles after the interaction with hairpins, gold nanoparticles and ATP. To construct such an assay system, two hairpin DNA probes with a short single-stranded DNA at the sticky end are utilized for interaction with gold nanoparticles...
March 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27813264/protein-charge-determination-and-implications-for-interactions-in-cell-extracts
#20
Ciara Kyne, Kiara Jordon, Dana I Filoti, Thomas M Laue, Peter B Crowley
Decades of dilute-solution studies have revealed the influence of charged residues on protein stability, solubility and stickiness. Similar characterizations are now required in physiological solutions to understand the effect of charge on protein behavior under native conditions. Toward this end, we used free boundary and native gel electrophoresis to explore the charge of cytochrome c in buffer and in Escherichia coli extracts. We find that the charge of cytochrome c was ∼2-fold lower than predicted from primary structure analysis...
February 2017: Protein Science: a Publication of the Protein Society
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