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https://www.readbyqxmd.com/read/28948693/dna-microcapsule-for-photo-triggered-drug-release-system
#1
Yukiko Kamiya, Yoshinobu Yamada, Takahiro Muro, Kazunori Matsuura, Hiroyuki Asanuma
In this study, we constructed spherical, photo-responsive microcapsules composed of three photo-switchable DNA strands. These strands first formed a three-way junction (TWJ) motif that further self-assembled to form microsphere through hybridization of the sticky-end regions of each branch. To serve as the photo-switch, multiple unmodified azobenzene (Azo) or 2,6-dimethyl-4-(methylthio)azobenzene (SDM-Azo) were introduced into the sticky-end regions via a D-threoninol linker. The DNA-capsule structure deformed upon trans-to-cis isomerization of Azo or SDM-Azo induced by specific light-irradiation...
September 25, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28914989/encapsulating-active-pharmaceutical-ingredients-in-self-assembling-adamantanes-with-short-dna-zippers
#2
Clemens Richert, Helmut Griesser, Alexander Schwenger
Formulating pharmaceutically active ingredients for drug delivery is a challenge. There is a need for new drug delivery systems that take up therapeutic molecules and release them into biological systems. We propose a novel mode of encapsulation that involves matrices formed through co-assembly of drugs with adamantane hybrids that feature four CG dimers as sticky ends. Such adamantanes are accessible via inexpensive solution-phase syntheses, and the resulting materials show attractive properties for controlled release...
September 15, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28821153/combination-of-microsized-mineral-particles-and-rosin-as-a-basis-for-converting-cellulosic-fibers-into-sticky-superhydrophobic-paper
#3
Xiaoyan Yu, Peiwen Bian, Yang Xue, Xueren Qian, Haipeng Yu, Wenshuai Chen, Xiaohai Hu, Peng Wang, Dong Wu, Qinghui Duan, Limei Li, Jing Shen, Yonghao Ni
The unique features of cellulosic paper including flexibility, biodegradability, and low cost enables it as a versatile, sustainable biomaterial for promising applications. In the paper industry, microsized mineral particles are widely used in the production of printing/writing paper grades, while rosin derived from trees is the earliest internal sizing agent for paper hydrophobication. On the basis of existing commercial practices associated with the use of mineral particles and rosin in paper production, we present a process concept of converting cellulosic fibers (paper-grade pulp) into "sticky" superhydrophobic paper involving the use of microsized mineral particles and rosin (a tree-derived natural product, mainly a mixture of resin acids, especially abietic acid with chemical formula of C19H29COOH)...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28815506/preparing-fosmid-mate-paired-libraries-using-cre-loxp-recombination
#4
Ze Peng, Jeff L Froula, Jan-Fang Cheng
Fosmid end sequencing has been widely utilized in genome sequence assemblies and genome structural variation studies. We have developed a new approach to construct fosmid paired-end libraries that is suitable for Illumina sequencing platform. This approach employs a newly modified fosmid vector (pFosClip) which contains two loxP sites with identical orientation and two inverse Illumina adaptor priming sites flanking the cloning site. DNA prepared from the fosmid library constructed with pFosClip can be treated with the Cre recombinase to remove most of the vector DNA, leaving only 107 bp of the vector sequence with insert DNA...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28813590/understanding-the-elementary-steps-in-dna-tile-based-self-assembly
#5
Shuoxing Jiang, Fan Hong, Huiyu Hu, Hao Yan, Yan Liu
Although many models have been developed to guide the design and implementation of DNA tile-based self-assembly systems with increasing complexity, the fundamental assumptions of the models have not been thoroughly tested. To expand the quantitative understanding of DNA tile-based self-assembly and to test the fundamental assumptions of self-assembly models, we investigated DNA tile attachment to preformed "multi-tile" arrays in real time and obtained the thermodynamic and kinetic parameters of single tile attachment in various sticky end association scenarios...
September 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28806061/construction-of-a-polyhedral-dna-12-arm-junction-for-self-assembly-of-wireframe-dna-lattices
#6
Ilenia Manuguerra, Guido Grossi, Rasmus P Thomsen, Jeppe Lyngsø, Jan S Pedersen, Jørgen Kjems, Ebbe S Andersen, Kurt V Gothelf
A variety of different tiles for the construction of DNA lattices have been developed since the structural DNA nanotechnology field was born. The majority of these are designed for the realization of close-packed structures, where DNA helices are arranged in parallel and tiles are connected through sticky ends. Assembly of such structures requires the use of cation-rich buffers to minimize repulsion between parallel helices, which poses limits to the application of DNA nanostructures. Wireframe structures, on the other hand, are less susceptible to salt concentration, but the assembly of wireframe lattices is limited by the availability of tiles and motifs...
September 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28764772/phlebotomine-sand-fly-survey-in-the-focus-of-leishmaniasis-in-madrid-spain-2012-2014-seasonal-dynamics-leishmania-infantum-infection-rates-and-blood-meal-preferences
#7
Estela González, Maribel Jiménez, Sonia Hernández, Inés Martín-Martín, Ricardo Molina
BACKGROUND: An unusual increase of human leishmaniasis cases due to Leishmania infantum is occurring in an urban area of southwestern Madrid, Spain, since 2010. Entomological surveys have shown that Phlebotomus perniciosus is the only potential vector. Direct xenodiagnosis in hares (Lepus granatensis) and rabbits (Oryctolagus cuniculus) collected in the focus area proved that they can transmit parasites to colonized P. perniciosus. Isolates were characterized as L. infantum. The aim of the present work was to conduct a comprehensive study of sand flies in the outbreak area, with special emphasis on P...
August 1, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28681900/tile-based-self-assembly-of-a-triple-helical-polysaccharide-into-cell-wall-like-mesoporous-nanocapsules
#8
Chaoxi Wu, Xiaoying Wang, Jianjing Wang, Zhen Zhang, Zhiping Wang, Yifei Wang, Shunqing Tang
Tile-based self-assembly is a robust system in the construction of three-dimensional DNA nanostructures but it has been rarely applied to other helical biopolymers. β-Glucan is an immunoactive natural polymer which exists in a triple helical conformation. Herein, we report that β-glucan, after modification using two types of short chain acyl groups, can self-assemble into tiles with inactivated sticky ends at the interface of two solvents. These tiles consist of a single layer of helices laterally aligned, and the sticky ends can be activated when a few acyl groups at the ends are removed; these tiles can further pack into mesoporous nanocapsules, in a similar process as the sticky DNA tiles pack into complex polyhedral nano-objects...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28654071/protocols-for-c-brick-dna-standard-assembly-using-cpf1
#9
Shi-Yuan Li, Guo-Ping Zhao, Jin Wang
CRISPR-associated protein Cpf1 cleaves double-stranded DNA under the guidance of CRISPR RNA (crRNA), generating sticky ends. Because of this characteristic, Cpf1 has been used for the establishment of a DNA assembly standard called C-Brick, which has the advantage of long recognition sites and short scars. On a standard C-Brick vector, there are four Cpf1 recognition sites - the prefix (T1 and T2 sites) and the suffix (T3 and T4 sites) - flanking biological DNA parts. The cleavage of T2 and T3 sites produces complementary sticky ends, which allow for the assembly of DNA parts with T2 and T3 sites...
June 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28652345/dna-cytoskeleton-for-stabilizing-artificial-cells
#10
Chikako Kurokawa, Kei Fujiwara, Masamune Morita, Ibuki Kawamata, Yui Kawagishi, Atsushi Sakai, Yoshihiro Murayama, Shin-Ichiro M Nomura, Satoshi Murata, Masahiro Takinoue, Miho Yanagisawa
Cell-sized liposomes and droplets coated with lipid layers have been used as platforms for understanding live cells, constructing artificial cells, and implementing functional biomedical tools such as biosensing platforms and drug delivery systems. However, these systems are very fragile, which results from the absence of cytoskeletons in these systems. Here, we construct an artificial cytoskeleton using DNA nanostructures. The designed DNA oligomers form a Y-shaped nanostructure and connect to each other with their complementary sticky ends to form networks...
July 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28590320/sticky-silicone-oil
#11
Hamouda Hamdi Ghoraba, Adel Galal Zaky, Haithem Mamoun Abd Al Fatah, Emad El Din Mohamed El Gemai, Mohamed Amin Heikal
OBJECTIVE: To report and analyze the phenomenon of sticky silicone oil on the retina at the time of its removal. BACKGROUND: Silicone oil has been used for more than 25 years as a long-term retinal tamponade in the surgical management of patients with complex retinal detachments. When a decision to remove silicone oil was made at the end of the tamponade period, the system involved a number of different materials: silicone oil, aqueous solution with or without remnants of perfluorocarbon liquid plus the material of the cannula used to aspirate Silicone oil...
August 2017: Retina
https://www.readbyqxmd.com/read/28573294/transition-from-nematic-to-gyroid-type-cubic-soft-self-assembly-by-side-chain-engineering-of-%C3%AF-conjugated-sticky-rods
#12
Marco Poppe, Changlong Chen, Helgard Ebert, Silvio Poppe, Marko Prehm, Christoph Kerzig, Feng Liu, Carsten Tschierske
A sequence of liquid crystalline phases, involving cybotactic nematics, a lamellar phase, bicontinuous cubics and triangular honeycombs, was observed for oligo(phenylene ethynylene) based X-shaped bolapolyphiles with two long lateral alkyl chains and sticky ends provided by glycerol groups. In the cubic phase with Ia3[combining macron]d lattice - which is tailored by alkyl chain engineering - the aromatic cores are organized on the gyroid minimal surface in 3D curved layers of almost parallel aligned π-conjugated rods...
June 21, 2017: Soft Matter
https://www.readbyqxmd.com/read/28514850/dna-dendrimer-streptavidin-nanocomplex-an-efficient-signal-amplifier-for-construction-of-biosensing-platforms
#13
Yan Zhao, Shichao Hu, Huaming Wang, Kaiwen Yu, Yan Guan, Xiaoyun Liu, Na Li, Feng Liu
We develop a DNA dendrimer-streptavidin (SA) nanocomplex as a novel signal amplifier to create biosensing platforms for disease-related species. The DNA dendrimer-SA nanocomplex is fabricated by cross-linking the nonlinear hybridization chain reaction based DNA dendrimer with the SA-coupled linker DNA and possesses multiple sticky ends, a high molecular weight, and a hyperbranched nanostructure with large numbers of DNA duplexes. Taking advantage of the DNA dendrimer-SA nanocomplex and a label-free quartz crystal microbalance (QCM) technology, we first construct a mass-sensitive QCM biosensing platform for nucleic acids, which displays high selectivity and sensitivity, with a detection limit of 0...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28504444/diversity-ecology-and-seasonality-of-sand-flies-diptera-psychodidae-of-the-jenin-district-palestinian-territories
#14
Samir S Sawalha, Asad Ramlawi, Ramzi M Sansur, Ibrahim Mohammad Salem, Zuhair S Amr
The diversity, ecology, and seasonality for sand flies from two localities in Jenin District, the Palestinian Territories, were studied. A total of 12,579 sand flies (5,420 Phlebotomus and 7,159 Sergentomyia) were collected during the study period. The genera Phlebotomus and Sergentomyia are represented by 13 and nine species and subspecies, respectively. Species account was given for all collected species. CDC light traps yielded 7,649 (60.8%) of the total captured sand flies, while sticky traps and aspirators contributed to 36...
June 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/28443918/ph-responsiveness-of-hydrogels-formed-by-telechelic-polyampholytes
#15
Margarita A Dyakonova, George Gotzamanis, Bart-Jan Niebuur, Natalya S Vishnevetskaya, Konstantinos N Raftopoulos, Zhenyu Di, Sergey K Filippov, Constantinos Tsitsilianis, Christine M Papadakis
We investigate the influence of pH on the rheological and structural properties of hydrogels formed by hydrophobic association of the sticky ends of the triblock terpolymer poly(methyl methacrylate)-b-poly(2-(diethylamino)ethyl methacrylate-co-methacrylic acid)-b-poly(methyl methacrylate) (PMMA-b-P(DEA-co-MAA)-b-PMMA). The middle block is a weak polyampholyte having a pH dependent charge density and sign, which enables tuning of the rheological and structural properties by pH variation. Small-angle neutron scattering (SANS) studies of solutions in D2O at 0...
April 26, 2017: Soft Matter
https://www.readbyqxmd.com/read/28334880/dna-nanostructures-constructed-with-multi-stranded-motifs
#16
Donglei Yang, Zhenyu Tan, Yongli Mi, Bryan Wei
Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The length of sticky ends can be adjusted to form different dihedral angles between the matching motifs, which corresponds to different connecting patterns. Moreover, the length of rigidity core can also be tuned to result in different dihedral angles between the component helices of a certain motif therefore different numbers of component helices...
April 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28211680/two-way-gold-nanoparticle-label-free-sensing-of-specific-sequence-and-small-molecule-targets-using-switchable-concatemers
#17
Longjiao Zhu, Xiangli Shao, Yunbo Luo, Kunlung Huang, Wentao Xu
A two-way colorimetric biosensor based on unmodified gold nanoparticles (GNPs) and a switchable double-stranded DNA (dsDNA) concatemer have been demonstrated. Two hairpin probes (H1 and H2) were first designed that provided the fuels to assemble the dsDNA concatemers via hybridization chain reaction (HCR). A functional hairpin (FH) was rationally designed to recognize the target sequences. All the hairpins contained a single-stranded DNA (ssDNA) loop and sticky end to prevent GNPs from salt-induced aggregation...
May 19, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28165224/programmable-dna-guided-artificial-restriction-enzymes
#18
Behnam Enghiad, Huimin Zhao
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA sequences at virtually any arbitrary site and generating defined sticky ends of varying length. Short DNA guides are used to direct PfAgo to target sites for cleavage at high temperatures (>87 °C) followed by reannealing of the cleaved single stranded DNAs...
February 6, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28115632/the-cctl-cpf1-assisted-cutting-and-taq-dna-ligase-assisted-ligation-method-for-efficient-editing-of-large-dna-constructs-in-vitro
#19
Chao Lei, Shi-Yuan Li, Jia-Kun Liu, Xuan Zheng, Guo-Ping Zhao, Jin Wang
As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly. Here, the Cpf1 cleavage sites were precisely characterized, where the cleavage site on the target strand was around the 22nd base relative to the protospacer adjacent motif site, but the cleavage on the non-target strand was affected by the spacer length. When the spacer length was 20 nt or longer, Cpf1 mainly cleaved around the 14th and the 18th bases on the non-target strand; otherwise, with a shorter spacer (i...
May 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28107938/nucleotide-base-analog-pyrrolo-deoxycytidine-as-fluorescent-probe-signal-for-enzyme-free-and-signal-amplified-nucleic-acids-detection
#20
Zhijuan Duan, Zhuo Li, Jianyuan Dai, Hongfei He, Dan Xiao
In the present work, an enzyme-free and signal amplified nucleic acids detection method based on the fluorescent nucleotide base analog pyrrolo-deoxycytidine (P-dC) and catalyzed hairpin assembly (CHA) has been developed. In the CHA signal amplification system, two hairpin auxiliary probes, H1 and H2, which containing a fluorescent P-dC at the end of the stem, respectively, were used as the fluorescent probes. The fluorescence of P-dC in H1 and H2 was quenched owing to the stacking interaction among the bases in the stem...
March 1, 2017: Talanta
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