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https://www.readbyqxmd.com/read/28545230/nanog-plays-a-hierarchical-role-in-the-transcription-network-regulating-the-pluripotency-and-plasticity-of-adipose-tissue-derived-stem-cells
#1
Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano
The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT...
May 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28545079/interleukin-like-emt-inducer-regulates-partial-phenotype-switching-in-mitf-low-melanoma-cell-lines
#2
Ken Noguchi, Annamarie C Dalton, Breege V Howley, Buckley J McCall, Akihiro Yoshida, J Alan Diehl, Philip H Howe
ILEI (FAM3C) is a secreted factor that contributes to the epithelial-to-mesenchymal transition (EMT), a cell biological process that confers metastatic properties to a tumor cell. Initially, we found that ILEI mRNA is highly expressed in melanoma metastases but not in primary tumors, suggesting that ILEI contributes to the malignant properties of melanoma. While melanoma is not an epithelial cell-derived tumor and does not undergo a traditional EMT, melanoma undergoes a similar process known as phenotype switching in which high (micropthalmia-related transcription factor) MITF expressing (MITF-high) proliferative cells switch to a low expressing (MITF-low) invasive state...
2017: PloS One
https://www.readbyqxmd.com/read/28545036/the-long-non-coding-rna-neat1-enhances-epithelial-to-mesenchymal-transition-and-chemoresistance-via-the-mir-34a-c-met-axis-in-renal-cell-carcinoma
#3
Fei Liu, Na Chen, Yanchun Gong, Ruihai Xiao, Weichao Wang, Zhengyue Pan
Long non-coding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in various cancers. Although the lncRNA nuclear enriched abundant transcript 1 (NEAT1) has been associated with tumorigenesis, its functions in renal cell carcinoma (RCC) have not been elucidated. We determined that NEAT1 is up-regulated in RCC tissue compared to corresponding non-tumor tissue. High NEAT1 expression was associated with tumor progression and poor survival in RCC patients. NEAT1 knockdown suppressed RCC cell proliferation by inhibiting cell cycle progression, and inhibited RCC cell migration and invasion by reversing the epithelial-to- mesenchymal transition phenotype...
May 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28544961/superficial-physicochemical-properties-of-polyurethane-biomaterials-as-osteogenic-regulators-in-human-mesenchymal-stem-cells-fates
#4
Mohsen Shahrousvand, Gity Mir Mohamad Sadeghi, Ehsan Shahrousvand, Marzieh Ghollasi, Ali Salimi
All of the cells' interactions are done through their surfaces. Evaluation of surface physicochemical scaffolds along with other factors is important and determines the fate of stem cells. In this work, biodegradable and biocompatible polyester/polyether based polyurethanes (PUs) were synthesized by polycaprolactone diol (PCL) and poly (tetra methylene ether) glycol (PTMEG) as the soft segment. To assess better the impact of surface parameters such as stiffness and roughness effects on osteogenic differentiation of the human mesenchymal stem cell (hMSC), the dimension effect of substrates was eliminated and two-dimensional membranes were produced by synthesized polyurethane...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544959/effect-of-different-mineralization-processes-on-in-vitro-and-in-vivo-bone-regeneration-and-osteoblast-macrophage-cross-talk-in-co-culture-system-using-dual-growth-factor-mediated-non-mulberry-silk-fibroin-grafted-poly-%C3%B0-caprolactone-nanofibrous-scaffold
#5
Promita Bhattacharjee, Tapas K Maiti, Debasis Bhattacharya, Samit Kumar Nandi
This study evaluates mineralized nanofibrous polymeric scaffolds at supporting in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs) and in vivo bone tissue regeneration. Co-electrospin, alternative soaking, and electrodeposition were used to introduce hydroxyapatite in non-mulberry silk fibroin grafted poly(Є-caprolactone) nanofibrous scaffolds. Bone morphogenic protein-2 and Transforming growth factor-β, at a potency ratio of 1:1, are covalently coupled onto the scaffolds. hMSCs proliferation and interactions are studied through MTT and Alamar blue assay and scanning electron and confocal microscopy...
May 16, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544815/fabrication-of-viable-and-functional-pre-vascularized-modular-bone-tissues-by-coculturing-mscs-and-huvecs-on-microcarriers-in-spinner-flasks
#6
Songjie Zhang, Min Zhou, Zhaoyang Ye, Yan Zhou, Wen-Song Tan
BACKGROUND AND AIM: Slow vascularization often impedes the viability and function of engineered bone replacements. Prevascularization is a promising way to solve this problem. In this study, a new process was developed by integrating microcarrier culture and coculture to fabricate pre-vascularized bone microtissues with mesenchymal stem cells (MSCs) and human umbilical vein endotheli-al cells (HUVECs). METHODOLOGY: Initially, coculture medium and cell ratio between MSCs and HUVECs were optimized in tissue culture plates concerning cell proliferation, osteogenesis and angiogenesis...
May 24, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28544786/mir-122-modification-enhances-the-therapeutic-efficacy-of-adipose-tissue-derived-mesenchymal-stem-cells-against-liver-fibrosis
#7
Guohua Lou, Ying Yang, Feifei Liu, Bingjue Ye, Zhi Chen, Min Zheng, Yanning Liu
Mesenchymal stem cell (MSC) transplantation alone may be insufficient for treatment of liver fibrosis because of complicated histopathological changes in the liver. Given that miR-122 plays an essential role in liver fibrosis by negatively regulating the proliferation and transactivation of hepatic stellate cells (HSCs), this study investigated whether miR-122 modification can improve the therapeutic efficacy of adipose tissue-derived MSCs in treating liver fibrosis. MiR-122-modified AMSCs (AMSC-122) were constructed through lentivirus-mediated transfer of pre-miR-122...
May 24, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28544661/early-passage-mesenchymal-stem-cells-display-decreased-radiosensitivity-and-increased-dna-repair-activity
#8
Po-Kuei Wu, Jir-You Wang, Cheng-Fong Chen, Kuang-Yu Chao, Ming-Chau Chang, Wei-Ming Chen, Shih-Chieh Hung
Cell therapies using human mesenchymal stem cells (MSCs) have received much attention in the past decade. In pursuit of the therapeutic potential of MSCs, cell expansion is required to generate a great number of cells with desired phenotype and functionality. Long-term expansion in vitro, however, can lead to altered functions. To explore the changes in DNA damage responses (DDR) in MSCs expanded, DDR pathways following irradiation were characterized in early- and late-passage bone marrow MSCs. Seventy-two hours after irradiation, the percentage of sub-G1 cells in early-passage MSCs did not change significantly...
June 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28544627/epithelial-to-mesenchymal-transition-transcription-factors-in-cancer-associated-fibroblasts
#9
REVIEW
Josep Baulida
Beyond inducing epithelial-to-mesenchymal transcription (EMT), transcriptional factors of the Snail, ZEB and Twist families (EMT-TFs) control global plasticity programs affecting cell stemness and fate. Literature addressing the reactivation of these factors in adult tumour cells is very extensive, as they enable cancer cell plasticity and fuel both tumour initiation and metastatic spread. Incipient data reveal that EMT-TFs are also expressed in fibroblasts, providing these with additional properties. Here, I will review recent reports on the expression of EMT-TFs in cancer-associated fibroblasts (CAFs)...
May 24, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28544576/atypical-adenoma-of-the-thyroid-diagnosed-as-anaplastic-cancer-by-cytopathology
#10
Hiromitsu Hatakeyama, Kimiko Hoshino, Kenji Mizoguchi, Takayoshi Suzuki, Kanako C Hatanaka, Yukie Yamaya, Satoshi Kano, Takatsugu Mizumachi, Akihiro Homma
Atypical adenoma of the thyroid is a rare form of tumor, and its accurate diagnosis prior to surgical resection is difficult as the histological and pathological morphologies are very similar to those of anaplastic thyroid carcinoma (ATC), and its anaplastic transformation remains to be elucidated. We reported a case of a 75-year-old female with a thyroid isthmus nodule diagnosed repeatedly by FNAC as anaplastic carcinoma. Both the first and second FNAC specimen slides showed a large number of scattered or aggregated atypical cells consisting of large, pleomorphic nuclei with irregular membranes, chromatin clumps and prominent nucleoli...
May 22, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28544508/enhancement-of-plasma-protein-adsorption-and-osteogenesis-of-hmscs-by-functionalized-siloxane-coatings-for-titanium-implants
#11
Maria Martínez-Ibáñez, N Sanjeeva Murthy, Yong Mao, Julio Suay, Marilo Gurruchaga, Isabel Goñi, Joachim Kohn
A series of sol-gel derived silicon based coatings were developed to improve the osseointegration of commercial titanium dental implants. The osseointegration starts with a positive interaction between the implant surface and surrounding tissues, which is facilitated by the adsorption of plasma proteins onto the biomaterial surface immediately after implantation. It is likely that the enhancement of protein adsorption to titanium implants leads to a better implant/tissue integration. In addition, silica based biomaterials have been shown to promote osteoblast differentiation...
May 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28544498/cancer-metastasis-through-the-prism-of-epithelial-to-mesenchymal-transition-in-circulating-tumor-cells
#12
REVIEW
Douglas S Micalizzi, Daniel A Haber, Shyamala Maheswaran
Metastasis of epithelial cancer cells to distal sites is a particularly critical stage of cancer progression that typically marks the incurability of the disease. It is governed by a complex series of events including invasion and intravasation of tumor cells into the stroma and blood, respectively. Epithelial to mesenchymal transition (EMT), a phenotypic change marked by the loss of epithelial characteristics and the acquisition of invasive mesenchymal properties, is implicated in the dissemination of tumor cells...
May 24, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28544462/biomimetic-macroporous-pcl-scaffolds-for-ex-vivo-expansion-of-cord-blood-derived-cd34-cells-with-feeder-cells-support
#13
Xiuwei Pan, Qiong Sun, Yuanhao Zhang, Haibo Cai, Yun Gao, Yongjia Shen, Weian Zhang
Ex vivo expansion of hematopoietic stem cells (HSCs) with most current methods can hardly satisfy clinical application requirement. While in vivo, HSCs efficiently self-renew in niche where they interact with 3D extracellular matrix and stromal cells. Therefore, co-cultures of CD34(+) cells and mesenchyme stem cells derived from human amniotic membrane (hAMSCs) on the basis of biomimetic macroporous three-dimensional (3D) poly(ε-caprolactone) (PCL) scaffolds are developed, where scaffolds and hAMSCs are applied to mimic structural and cellular microenvironment of HSCs...
May 24, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28544452/a-conserved-role-for-vegf-signaling-in-specification-of-homologous-mesenchymal-cell-types-positioned-at-spatially-distinct-developmental-addresses-in-early-development-of-sea-urchins
#14
Eric M Erkenbrack, Elizabeth Petsios
Comparative studies of early development in echinoderms are revealing the tempo and mode of alterations to developmental gene regulatory networks and to the cell types they specify. In euechinoid sea urchins, skeletogenic mesenchyme (SM) ingresses prior to gastrulation at the vegetal pole and aligns into a ring-like array with two bilateral pockets of cells, the sites where spiculogenesis will later occur. In cidaroid sea urchins, the anciently diverged sister clade to euechinoid sea urchins, a homologous SM cell type ingresses later in development, after gastrulation has commenced, and consequently at a distinct developmental address...
May 23, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28544415/inertial-microfluidic-cell-stretcher-imcs-fully-automated-high-throughput-and-near-real-time-cell-mechanotyping
#15
Yanxiang Deng, Steven P Davis, Fan Yang, Kevin S Paulsen, Maneesh Kumar, Rebecca Sinnott DeVaux, Xianhui Wang, Douglas S Conklin, Assad Oberai, Jason I Herschkowitz, Aram J Chung
Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure cell mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming and labor-intensive processes, not allowing techniques to be practically used for cell biology research applications...
May 23, 2017: Small
https://www.readbyqxmd.com/read/28544403/serine-protease-inhibitor-kazal-type-1-spink1-downregulates-e-cadherin-and-induces-emt-of-hepatoma-cells-to-promote-hepatocellular-carcinoma-metastasis-via-the-mek-erk-signaling-pathway
#16
Ying Hai-Yan, Gong Chao-Jie, Feng Yi, Jing Da-Dao, Lun-Gen Lu
OBJECTIVE: To investigate serine protease inhibitor Kazal type 1(SPINK1) expression and its influence on the prognosis of human hepatocellular carcinoma(HCC) and to explore the underlying molecular mechanisms involved. METHODS: Eighty patients with HCC who underwent curative resection were followed-up with a median observation time of 58.6 months.SPINK1expression was detected in the primary HCC samples by immunohistochemistry . Its role in tumor invasion and metastasis was evaluated in vitro by gene silence using siRNA-mediated approach, rSPINK1 and U0126(an inhibitor of MEK/ERK)...
May 23, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28544201/influence-of-highly-porous-electrospun-plga-pcl-nha-fibrous-scaffolds-on-the-differentiation-of-tooth-bud-cells-in-vitro
#17
Xinjie Cai, Sofie Ten Hoopen, Weibo Zhang, Charles Yi, Wanxun Yang, Fang Yang, John A Jansen, X Frank Walboomers, Pamela C Yelick
In this study, we investigated the use of three-dimensional electrospun poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) scaffolds seeded and cultured with postnatal dental cells, for improved dental tissue regeneration. Wet-electrospinning combined with ultrasonic treatment was studied as a method to enhance scaffold porosity and to promote cell-cell interactions. We also investigated whether nano-hydroxyapatite (nHA) incorporation could enhance dental cell differentiation. All scaffolds were seeded with human tooth pulp-derived dental mesenchymal (hDM) cells, or a combination of hDM and pig dental epithelial (pDE) cells, cultured for up to 28 days...
May 24, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28544005/hiv-1-p55-gag-protein-induces-senescence-of-human-bone-marrow-mesenchymal-stem-cells-and-reduces-their-capacity-to-support-expansion-of-hematopoietic-stem-cells-in-vitro
#18
Ya-Hong Yuan, Shan-Shan Zhao, Xiao-Li Wang, Zhi-Ping Teng, Dong-Sheng Li, Yi Zeng
Patients with human immunodeficiency virus-1 (HIV-1) infection often present with hematopoietic failure. As the important hematopoietic support cells in the bone marrow (BM), the bone marrow mesenchymal stem cells (BMSCs) can be impacted by HIV proteins that are released by infected cells within BM. In this study, we tested whether HIV protein p55-gag could induce senescence of BMSCs and reduce their capacity to support expansion of hematopoietic stem cells in vitro. BMSCs were chronically treated with p55-gag (BMSCgag ) for up to 20 days, and their proliferative activity and senescence makers were compared to nontreated cells (BMSCcon )...
May 25, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28543986/a-case-of-primordial-odontogenic-tumor-a-new-entity-in-the-latest-who-classification-2017
#19
Toshinori Ando, Madhu Shrestha, Takashi Nakamoto, Kaori Uchisako, Sachiko Yamasaki, Koichi Koizumi, Ikuko Ogawa, Mutsumi Miyauchi, Takashi Takata
Primordial odontogenic tumor (POT) is a rare lesion in the jaw which has been included as a new entity of benign mixed epithelial and mesenchymal odontogenic tumour in the latest World Health Organization (WHO) classification (2017). Only seven cases have been reported. It typically occurs in the posterior mandible. We report an additional case of POT in the maxilla of an 8-year-old girl presenting with an asymptomatic buccal enlargement. A well-defined, unilocular, radiolucent lesion was observed radiographically...
May 25, 2017: Pathology International
https://www.readbyqxmd.com/read/28543623/regulatory-effect-of-microrna-34a-on-osteogenesis-and-angiogenesis-in-glucocorticoid-induced-osteonecrosis-of-the-femoral-head
#20
Xiaolong Zha, Bolin Sun, Rufan Zhang, Chen Li, Zuoqin Yan, Jifei Chen
BACKGROUND AND AIMS: Glucocorticoid-induced osteonecrosis of the femoral head (GIOFH) is a common and devastating orthopedic disease, and its underlying mechanism remains unclear. The aim of this study was to determine the role of microRNA-34a (mir-34a) in GIOFH. METHODS: C57 mouse mesenchymal stem cells (mMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured with dexamethasone (Dex). A total of 48 adult rats were treated with glucocorticoids, and after the onset of GIOFH, each femoral head was removed...
May 20, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
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