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Integrin alpha 7

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https://www.readbyqxmd.com/read/29760566/-itga7-functions-as-a-tumor-suppressor-and-regulates-migration-and-invasion-in-breast-cancer
#1
Adheesh Bhandari, Erjie Xia, Yuying Zhou, Yaoyao Guan, Jingjing Xiang, Lingguo Kong, Yinghao Wang, Fan Yang, Ouchen Wang, Xiaohua Zhang
Background: Breast cancer is the most common malignancy in women and the underlying mechanism of breast cancer cell metastasis is still far from uncover. Integrin subunit alpha 7 ( ITGA7 ) is a functioning protein. It has been detected in many malignancies. But the function of ITGA7 in breast cancer is not clear. Our aim is to explore ITGA7 expression and its role in breast cancer. Methods: Real-time PCR was performed to determine ITGA7 expression in BC tissues and normal adjacent tissues...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/29674419/comparative-study-of-the-novel-rgd-motif-containing-itg%C3%AE-v%C3%AE-6-binding-peptides-sflap3-and-sfitgv6-for-diagnostic-application-in-hnscc
#2
Saskia Roesch, Thomas Lindner, Max Sauter, Anastasia Loktev, Paul Flechsig, Martin Müller, Walter Mier, Rolf Warta, Gerhard Dyckhoff, Christel Herold-Mende, Uwe Haberkorn, Annette Altmann
Alpha v beta 6 (αvβ6) integrin is overexpressed by several carcinomas, and thus considered as target for diagnostic imaging and anti-cancer therapies. Recently, we presented SFITGv6 as a novel potential tracer for imaging and targeted therapy of αvβ6 integin-positive carcinomas. Here, we analyzed affinity and specificity of five native αvβ6 integrin-specific binders in comparison to SFITGv6. Methods: Sunflower trypsin inhibitor (SFTI)-1-based peptides containing arginine-glycine-aspartic acid (RGD) motif-spanning octamers of fibronectin (SFFN1), tenascin C (SFTNC), vitronectin (SFVTN), and latency-associated peptides (LAP) 1 (SFLAP1), and 3 (SFLAP3) were synthesized and their binding potential to αvβ6 integrin-expressing head and neck squamous cell carcinoma (HNSCC) cell lines was evaluated...
April 19, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29656994/an-ex-vivo-abomasal-ovine-model-to-study-the-immediate-immune-response-in-the-context-of-haemonchus-contortus-larval-stage
#3
Saeed El-Ashram, Cuiping Li, Fathi Abouhajer, Rashid Mehmood, Ibrahim Al Nasr, Yinghui Zhang, Tang Lu, Ding Yili, Xun Suo, Zhang Haoji, Zhili Li, Shujian Huang
We have set up an ex vivo ovine abomasal model, which can mimic the multicellular process to explore the early steps in haemonchine nematode infection using RNA-seq technology. Ovine abomasal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. Atotal of 233 were substantially induced genes between L4 -inoculated and uninoculated-control tissues, respectively. However, a total of 14 were considerably down-regulated genes between the 51 aforementioned tissues...
April 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29547664/overlapping-expression-patterns-and-functions-of-three-paralogous-p5b-atpases-in-caenorhabditis-elegans
#4
Jeffrey Zielich, Elena Tzima, Eva Ayla Schröder, Faten Jemel, Barbara Conradt, Eric J Lambie
P5B ATPases are present in the genomes of diverse unicellular and multicellular eukaryotes, indicating that they have an ancient origin, and that they are important for cellular fitness. Inactivation of ATP13A2, one of the four human P5B ATPases, leads to early-onset Parkinson's disease (Kufor-Rakeb Syndrome). The presence of an invariant PPALP motif within the putative substrate interaction pocket of transmembrane segment M4 suggests that all P5B ATPases might have similar transport specificity; however, the identity of the transport substrate(s) remains unknown...
2018: PloS One
https://www.readbyqxmd.com/read/29325889/early-cell-response-of-osteogenic-cells-on-differently-modified-implant-surfaces-sequences-of-cell-proliferation-adherence-and-differentiation
#5
Sebastian Blatt, Andreas Max Pabst, Eik Schiegnitz, Marco Hosang, Thomas Ziebart, Christian Walter, Bilal Al-Nawas, Marcus Oliver Klein
OBJECTIVES: Osseointegration of dental implants is a crucial prerequisite for long-term survival. Therefore, surface modifications are needed to interact with the extracellular environment and to trigger osteogenic cell responses such as cell proliferation, adherence, and differentiation. The purpose of this study was to investigate different surface modifications in vitro over 2 weeks. MATERIALS AND METHODS: Commercially available cells from a human osteogenic cell line (HHOB-c) were cultivated on the following surfaces: titanium with smooth surfaces (polished titanium (P), machined titanium (M), polyetheretherketone (Peek)), titanium with rough and hydrophilised surfaces (acid etched titanium (A), sandblasted acid etched titanium (SA and SA2), sandblasted acid etched hydrophilised (SAH), titanium plasma painted titanium (TPS)), titanium with calcium phosphate-containing surfaces (titanium plasma painted calcium phosphate modified titanium (TPS-CaP), sandblasted calcium phosphate modified titanium (S-CaP), sandblasted acid etched calcium phosphate modified titanium (SA-CaP)), and zirconium-oxide (yttrium amplified zirconium (Z), yttrium amplified Ca2+ delivering zirconium (Z-Ca))...
March 2018: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29322079/fibroblast-and-keratinocyte-gene-expression-following-exposure-to-extracts-of-neem-plant-azadirachta-indica
#6
Takao Someya, Katsura Sano, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3)...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29306866/mechanical-stress-potentiates-the-differentiation-of-periodontal-ligament-stem-cells-into-keratocytes
#7
Jialin Chen, Wei Zhang, Ludvig J Backman, Peyman Kelk, Patrik Danielson
AIMS: To explore the role of corneal-shaped static mechanical strain on the differentiation of human periodontal ligament stem cells (PDLSCs) into keratocytes and the possible synergistic effects of mechanics and inducing medium. METHODS: PDLSCs were exposed to 3% static dome-shaped mechanical strain in a Flexcell Tension System for 3 days and 7 days. Keratocyte phenotype was determined by gene expression of keratocyte markers. Keratocyte differentiation (inducing) medium was introduced in the Flexcell system, either continuously or intermittently combined with mechanical stimulation...
April 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29228930/gene-expression-analysis-in-asthma-using-a-targeted-multiplex-array
#8
Christopher D Pascoe, Ma'en Obeidat, Bryna A Arsenault, Yunlong Nie, Stephanie Warner, Dorota Stefanowicz, Samuel J Wadsworth, Jeremy A Hirota, S Jasemine Yang, Delbert R Dorscheid, Chris Carlsten, Tillie L Hackett, Chun Y Seow, Peter D Paré
BACKGROUND: Gene expression changes in the structural cells of the airways are thought to play a role in the development of asthma and airway hyperresponsiveness. This includes changes to smooth muscle contractile machinery and epithelial barrier integrity genes. We used a targeted gene expression arrays to identify changes in the expression and co-expression of genes important in asthma pathology. METHODS: RNA was isolated from the airways of donor lungs from 12 patients with asthma (8 fatal) and 12 non-asthmatics controls and analyzed using a multiplexed, hypothesis-directed platform to detect differences in gene expression...
December 11, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/29172866/radiosensitization-of-ultrasmall-gnp-peg-crgdfk-in-alts1c1-exposed-to-therapeutic-protons-and-kilovoltage-and-megavoltage-photons
#9
Milad Enferadi, Sheng-Yung Fu, Ji-Hong Hong, Chuan-Jong Tung, Tsi-Chian Chao, Shiaw-Pyng Wey, Chun-Hui Chiu, Chun-Chieh Wang, Mahdi Sadeghi
PURPOSE: One of the promising radiosensitizers is the ultrasmall gold nanoparticle (GNP) with a hydrodynamic diameter <3 nm. We studied functionalized ultrasmall GNPs (1.8 nm diameter) coated by polyethylene glycol (PEG) and conjugated with cyclic RGDfK (2.6 nm hydrodynamic diameter) for targeting of alpha(v) beta(3) integrin (αv β3 ) in the murine ALTS1C1 glioma cell line. MATERIALS AND METHODS: We investigated the uptake, toxicity and radiosensitivity of GNP-PEG-cRGDfKs in ALTS1C1 cells exposed to protons, kilovoltage photons and megavoltage photons...
February 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28919876/peptide-composition-of-stroke-causing-emboli-correlate-with-serum-markers-of-atherosclerosis-and-inflammation
#10
Neal M Rao, Joseph Capri, Whitaker Cohn, Maram Abdaljaleel, Lucas Restrepo, Jeffrey A Gornbein, William H Yong, David S Liebeskind, Julian P Whitelegge
INTRODUCTION: The specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible that the composition of the thrombus from which an embolus originated will have distinctive molecular characteristics reflective of the underlying pathophysiology. We used mass spectrometry to evaluate the protein composition of retrieved emboli from patients with differing stroke etiologies and correlated the protein levels to serum predictors of atherosclerosis...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28441661/defective-platelet-activation-and-bleeding-complications-upon-cholestasis-in-mice
#11
Nina Sarah Gowert, Meike Klier, Maria Reich, Friedrich Reusswig, Lili Donner, Verena Keitel, Dieter Häussinger, Margitta Elvers
BACKGROUND/AIMS: Platelets are essential mediators of hemostasis to avoid excessive blood loss. Cirrhosis and chronic liver diseases are characterized by alterations in hemostasis. Alterations in the secondary hemostasis have been well studied, while defects in primary hemostasis, especially the consequences of cholestatic liver disease on platelet function are not well defined. METHODS: After bile duct ligation (BDL) platelet activation and thrombus formation were analyzed in mice...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28439079/translation-but-not-transfection-limits-clinically-relevant-exogenous-mrna-based-induction-of-alpha-4-integrin-expression-on-human-mesenchymal-stem-cells
#12
Adam Nowakowski, Anna Andrzejewska, Johannes Boltze, Franziska Nitzsche, Li-Li Cui, Jukka Jolkkonen, Piotr Walczak, Barbara Lukomska, Miroslaw Janowski
Mesenchymal stem cells (MSCs) represent promising resource of cells for regenerative medicine in neurological disorders. However, efficient and minimally invasive methods of MSCs delivery to the brain still have to be developed. Intra-arterial route is very promising, but MSCs are missing machinery for diapedesis through blood-brain barrier. Thus, here we have tested a mRNA-based method to induce transient expression of ITGA4, an adhesion molecule actively involved in cell extravasation. We observed that transfection with an ITGA4-mRNA construct bearing a conventional cap analogue (7-methylguanosine) failed to produce ITGA4 protein, but exogenous ITGA4-mRNA was detected in transfected MSCs...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28314844/abituzumab-targeting-of-%C3%AE-v-class-integrins-inhibits-prostate-cancer-progression
#13
Yuan Jiang, Jinlu Dai, Zhi Yao, Greg Shelley, Evan T Keller
Integrins that contain an integrin αV subunit contribute to multiple functions that promote cancer progression. The goal of this study was to determine whether abituzumab (DI17E6, EMD 525797), a humanized monoclonal antibody (mAb) against integrin αV impacts, prostate cancer progression. To evaluate this, prostate cancer cells were treated with DI17E6 and its effects on proliferation, apoptosis, cell-cycle, adhesion, detachment, migration, invasion and phosphorylation of downstream targets, including FAK, Akt, and ERK, were determined...
July 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28024362/controlled-sub-nanometer-epitope-spacing-in-a-three-dimensional-self-assembled-peptide-hydrogel
#14
E Thomas Pashuck, Benoît J R Duchet, Catherine S Hansel, Stephanie A Maynard, Lesley W Chow, Molly M Stevens
Cells in the body use a variety of mechanisms to ensure the specificity and efficacy of signal transduction. One way that this is achieved is through tight spatial control over the position of different proteins, signaling sequences, and biomolecules within and around cells. For instance, the extracellular matrix protein fibronectin presents RGDS and PHSRN sequences that synergistically bind the α5β1 integrin when separated by 3.2 nm but are unable to bind when this distance is >5.5 nm.1 Building biomaterials to controllably space different epitopes with subnanometer accuracy in a three-dimensional (3D) hydrogel is challenging...
December 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/27890854/association-between-low-trough-levels-of-vedolizumab-during-induction-therapy-for-inflammatory-bowel-diseases-and-need-for-additional-doses-within-6-months
#15
Nicolas Williet, Gilles Boschetti, Marion Fovet, Thomas Di Bernado, Pierre Claudez, Emilie Del Tedesco, Camille Jarlot, Leslie Rinaldi, Anne Berger, Jean-Marc Phelip, Bernard Flourie, Stéphane Nancey, Stéphane Paul, Xavier Roblin
BACKGROUND & AIMS: We investigated whether serum trough levels of vedolizumab, a humanized monoclonal antibody against integrin α4β7, during the induction phase of treatment can determine whether patients will need additional doses (optimization of therapy) within the first 6 months. METHODS: We conducted an observational study of 47 consecutive patients with Crohn's disease (CD; n = 31) or ulcerative colitis (UC; n = 16) who had not responded to 2 previous treatment regimens with antagonists of tumor necrosis factor and were starting therapy with vedolizumab at 2 hospitals in France, from June 2014 through April 2016...
November 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27660340/the-complexity-of-alpha-e-beta-7-blockade-in-inflammatory-bowel-diseases
#16
REVIEW
Carolijn Smids, Carmen S Horjus Talabur Horje, Femke van Wijk, Ellen G van Lochem
Monoclonal antibodies targeting integrins are emerging as new treatment option in inflammatory bowel diseases. Integrins are molecules involved in cell adhesion and signalling. After the successful introduction of anti-α4β7, currently anti-β7 is under evaluation in a phase three trial. Anti-β7 blocks both α4β7/MAdCAM-1 and αEβ7/E-cadherin interaction, targeting both the homing to and the retention in the gut of potential pathological T cells. Since the physiological and potential pathological roles of immune cells expressing αEβ7 are less distinct than of those expressing α4β7, an overview of the current state of knowledge on αEβ7 in mice and humans in both health and inflammatory bowel diseases is presented here, also addressing the potential consequences of anti-β7 treatment...
April 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/27535240/hyperglycemia-reduces-integrin-subunits-alpha-v-and-alpha-5-on-the-surface-of-dermal-fibroblasts-contributing-to-deficient-migration
#17
Maira Estanislau S Almeida, Kelly S Monteiro, Ellen E Kato, Sandra C Sampaio, Tarcio T Braga, Niels O S Câmara, Marcelo L Lamers, Marinilce F Santos
Deficient wound healing is a common multifactorial complication in diabetic patients, but the cellular and molecular mechanisms involved are poorly defined. In the present study, we analyzed the effects of hyperglycemia on integrins expression in rat dermal fibroblasts and addressed its role in cell adhesion and migration. Diabetes Mellitus was induced in rats by streptozotocin injection and maintained for 30 days. Primary cultures of dermal fibroblasts from control and diabetic rats were maintained under low glucose (5 mM D-glucose) or high glucose (30 mM D-glucose) for 7 days...
October 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27269969/features-of-liver-tissue-remodeling-in-intestinal-failure-during-and-after-weaning-off-parenteral-nutrition
#18
Annika Mutanen, Jouko Lohi, Timo Sorsa, Hannu Jalanko, Mikko P Pakarinen
BACKGROUND: Intestinal failure is associated frequently with liver injury, which persists after weaning off parenteral nutrition. We compared features of liver remodeling in intestinal failure during and after weaning off parenteral nutrition. METHODS: Liver biopsies and serum samples were obtained from 25 intestinal failure patients at a median age of 9.7 years (interquartile range: 4.6-18) and from age-matched control patients. Seven patients had been receiving parenteral nutrition for 53 months (22-160), and 18 patients had been weaned off parenteral nutrition 6...
September 2016: Surgery
https://www.readbyqxmd.com/read/27105860/osteogenic-gene-array-of-osteoblasts-cultured-on-a-novel-osteoinductive-biphasic-calcium-phosphate-bone-grafting-material
#19
Richard J Miron, Yuang Shuang, Dieter D Bosshardt, Jordi Caballé-Serrano, Fatiha Chandad, Yufeng Zhang
OBJECTIVES: Recently, novel biphasic calcium phosphate (BCP) scaffolds have emerged as a new class of bone grafts with osteoinductive potential demonstrating the ability to form ectopic bone in extra-skeletal sites. The aim of the present study was to perform an osteogenic gene array to target possible genes responsible for eliciting the changes in cell expression responsible for inducing osteoblast differentiation. MATERIALS AND METHODS: Human MG63 osteoblast-like cells were seeded for 24 h on tissue culture plastic or osteoinductive BCP particles and analyzed for upregulated genes using an osteogenesis super-array...
April 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/26948838/efficacy-and-safety-of-vedolizumab-in-the-treatment-of-ulcerative-colitis
#20
REVIEW
Eugeni Domènech, Javier P Gisbert
Integrins play a crucial role in the development and maintenance of the inflammatory process in patients with inflammatory bowel disease. Vedolizumab is a humanized monoclonal antibody with a predominantly gastrointestinal effect. It specifically inhibits leukocyte integrin α4 β7 , thus preventing its interaction with mucosal vascular addressin cell adhesion molecule 1(MAdCAM-1), which is involved in the migration of lymphocytes from the blood stream to the intestinal tissue. Vedolizumab is indicated in the treatment of moderate to severe active Crohn's disease and ulcerative colitis in adult patients with poor response, loss of response, or intolerance to conventional treatment or to tumour necrosis factor alpha (TNF-α) antagonists...
December 2016: Gastroenterología y Hepatología
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