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https://www.readbyqxmd.com/read/28211531/proteomic-analysis-of-urinary-extracellular-vesicles-from-high-gleason-score-prostate-cancer
#1
Kazutoshi Fujita, Hideaki Kume, Kyosuke Matsuzaki, Atsunari Kawashima, Takeshi Ujike, Akira Nagahara, Motohide Uemura, Yasushi Miyagawa, Takeshi Tomonaga, Norio Nonomura
Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n = 6], GS 6 PCa [n = 6], or GS 8-9 PCa [n = 6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. We identified 4710 proteins and quantified 3528 proteins in the urinary EVs. Eleven proteins increased in patients with PCa compared to those with negative biopsy (ratio >1...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205552/a-class-of-extracellular-vesicles-from-breast-cancer-cells-activates-vegf-receptors-and-tumour-angiogenesis
#2
Qiyu Feng, Chengliang Zhang, David Lum, Joseph E Druso, Bryant Blank, Kristin F Wilson, Alana Welm, Marc A Antonyak, Richard A Cerione
Non-classical secretory vesicles, collectively referred to as extracellular vesicles (EVs), have been implicated in different aspects of cancer cell survival and metastasis. Here, we describe how a specific class of EVs, called microvesicles (MVs), activates VEGF receptors and tumour angiogenesis through a unique 90 kDa form of VEGF (VEGF90K). We show that VEGF90K is generated by the crosslinking of VEGF165, catalysed by the enzyme tissue transglutaminase, and associates with MVs through its interaction with the chaperone Hsp90...
February 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28202520/evolution-of-cancer-stem-like-cells-in-endocrine-resistant-metastatic-breast-cancers-is-mediated-by-stromal-microvesicles
#3
Pasquale Sansone, Marjan Berishaj, Vinagolu K Rajasekhar, Claudio Ceccarelli, Qing Chang, Antonio Strillacci, Claudia Savini, Lauren Shapiro, Robert Bowman, Chiara Mastroleo, Sabrina De Carolis, Laura Daly, Alberto Benito-Martin, Fabiana Perna, Nicola Fabbri, John H Healey, Enzo Spisni, Monica Cricca, David Lyden, Massimiliano Bonafé, Jacqueline Bromberg
The hypothesis that microvesicle (MV)-mediated microRNA transfer converts non-cancer stem cells into cancer stem cells (CSCs) leading to therapy resistance remains poorly investigated. Here we provide direct evidence supporting this hypothesis, by demonstrating how MV derived from cancer associated fibroblasts (CAF) transfer miR-221 to promote hormonal therapy resistance (HTR) in models of luminal breast cancer. We determined that CAF-derived MV horizontally transferred miR221 to tumor cells and, in combination with hormone therapy activated an ERlo/Notchhi feed-forward loop responsible for the generation of CD133hi CSC...
February 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28202504/expression-profiling-of-circulating-microvesicles-reveals-intercellular-transmission-of-oncogenic-pathways
#4
Gloria Milani, Tobia Lana, Silvia Bresolin, Sanja Aveic, Anna Pasto, Chiara Frasson, Geertruy Te Kronnie
: Circulating microvesicles (MVs) have been described as important players in cell-to-cell communication carrying biological information under normal or pathological condition. MVs released by cancer cells may incorporate diverse biomolecules (e.g. active lipids, proteins and RNA) which can be delivered and internalized by recipient cells, potentially altering gene expression of recipient cells and eventually impacting disease progression. Leukemia in vitro model systems were used to investigate MVs as vehicles of protein-coding messages...
February 15, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28198167/signal-peptide-peptidase-encoded-by-hm13-contributes-to-tumor-progression-by-affecting-egfrviii-secretion-profiles-in-glioblastoma
#5
Jian-Wei Wei, Jin-Quan Cai, Chuan Fang, Yan-Li Tan, Kai Huang, Chao Yang, Qun Chen, Chuan-Lu Jiang, Chun-Sheng Kang
BACKGROUND AND AIMS: EGFRvIII is the most prevalent glioblastoma mutation, occurring in more than 25% of glioblastomas. EGFRvIII cells release microvesicles that contain proteins, miRNAs, and mRNAs that enhance the growth and survival of surrounding tumor cells. However, little is known about the maturation process and regulatory mechanisms of secreted vesicles in EGFRvIII cells. METHODS: Signal peptide peptidase (SPP) provides a fascinating mechanism for protein cleavage and subsequent dislocation in the endoplasmic reticulum transmembrane domain...
March 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/28197629/rab27a-rab27b-and-vps36-are-downregulated-in-advanced-prostate-cancer-and-show-functional-relevance-in-prostate-cancer-cells
#6
Thomas Stefan Worst, Yannic Meyer, Maria Gottschalt, Cleo-Aron Weis, Jost von Hardenberg, Christine Frank, Annette Steidler, Maurice Stephan Michel, Philipp Erben
Paracrine and long-range signaling via extracellular vesicles, such as exosomes and microvesicles, is deemed crucial for tumorigenesis, invasion and spread of solid tumors. The ESCRT machinery (endosomal sorting complexes required for transport) and Rab-proteins act as key players in vesicular trafficking and secretion. Yet, their role in prostate cancer (PCa) is unknown. Therefore, this study aimed to elucidate the relevance of these components in PCa. In silico reanalysis of genes with known involvement in vesicular trafficking and secretion in an existing microarray dataset revealed low expression of RAB27A, RAB27B and VPS36 to be predictive for reduced BCR-free survival in patients with localized PCa (p=0...
February 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28194184/signal-factors-secreted-by-2d-and-spheroid-mesenchymal-stem-cells-and-by-cocultures-of-mesenchymal-stem-cells-derived-microvesicles-and-retinal-photoreceptor-neurons
#7
Lili Xie, Mao Mao, Liang Zhou, Lusi Zhang, Bing Jiang
We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons. We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers. MVs isolated from all 3 culture conditions were incubated with 661W cells. Levels of 51 signal factors in conditioned medium from those cultured conditions were quantified with bead-based assay...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28186501/intrinsic-repair-protects-cells-from-pore-forming-toxins-by-microvesicle-shedding
#8
Matthew Romero, Michelle Keyel, Guilan Shi, Pushpak Bhattacharjee, Robyn Roth, John E Heuser, Peter A Keyel
Pore-forming toxins (PFTs) are used by both the immune system and by pathogens to disrupt cell membranes. Cells attempt to repair this disruption in various ways, but the exact mechanism(s) that cells use are not fully understood, nor agreed upon. Current models for membrane repair include (1) patch formation (e.g., fusion of internal vesicles with plasma membrane defects), (2) endocytosis of the pores, and (3) shedding of the pores by blebbing from the cell membrane. In this study, we sought to determine the specific mechanism(s) that cells use to resist three different cholesterol-dependent PFTs: Streptolysin O, Perfringolysin O, and Intermedilysin...
February 10, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28186467/effect-of-the-microenvironment-on-mesenchymal-stem-cells-paracrine-signalling-opportunities-to-engineer-the-therapeutic-effect
#9
Gina Diamanta Kusuma, James Carthew, Rebecca Lim, Jessica Ellen Frith
Cues from the extracellular environment, including physical stimuli, are well known to affect mesenchymal stem cells (MSCs) properties in terms of proliferation and differentiation. Many therapeutic strategies are now targeting this knowledge to increase the efficacy of cell therapies, typically employed to repair tissue functions in the event of injury, either by direct engraftment into the target tissue or differentiation into mature tissues. However, it is now envisioned that harnessing the repertoire of factors secreted by MSCs (termed the secretome) may provide an alternate to these cell therapies...
February 10, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28185192/apoptotic-bodies-selective-detection-in-extracellular-vesicles
#10
Paul Hauser, Sha Wang, Vladimir V Didenko
Normal and dying cells release various types of membrane-bound vesicles including microvesicles, exosomes, and apoptotic bodies. These vesicles play important roles in intercellular communication and signal transduction. However, their diverse forms and subtypes fluctuate in size and other properties. In result current purification approaches do not fully discriminate between different categories of extracellular vesicles. Here, we present a fluorescence technique that specifically identifies apoptotic bodies in preparations of microvesicles, exosomes, and other extracellular vesicles...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28182086/urinary-microvesicle-bound-uromodulin-a-potential-molecular-biomarker-in-diabetic-kidney-disease
#11
Neng-Jun Lou, Yi-Hong Ni, Hong-Ying Jia, Jing-Ti Deng, Lu Jiang, Feng-Jie Zheng, Ai-Li Sun
This study was designed to investigate the changes of urinary microvesicle-bound uromodulin and total urinary uromodulin levels in human urine and the correlations with the severity of diabetic kidney disease (DKD). 31 healthy subjects without diabetes and 100 patients with type 2 diabetes mellitus (T2DM) were included in this study. The patients with T2DM were divided into three groups based on the urinary albumin/creatinine ratio (UACR): normoalbuminuria group (DM, n = 46); microalbuminuria group (DN1, n = 32); and macroalbuminuria group (DN2, n = 22)...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28168203/microvesicles-correlated-with-components-of-metabolic-syndrome-in-men-with-type-2-diabetes-mellitus-and-lowered-testosterone-levels-but-were-unaltered-by-testosterone-therapy
#12
Jaco Botha, Line Velling Magnussen, Morten Hjuler Nielsen, Tine Bo Nielsen, Kurt Højlund, Marianne Skovsager Andersen, Aase Handberg
Aims. To investigate how circulating microvesicle phenotypes correlate with insulin sensitivity, body composition, plasma lipids, and hepatic fat accumulation. We hypothesized that changes elicited by testosterone replacement therapy are reflected in levels of microvesicles. Methods. Thirty-nine type 2 diabetic males with lowered testosterone levels were assigned to either testosterone replacement therapy or placebo and evaluated at baseline and after 24 weeks. Microvesicles were analysed by flow cytometry and defined as lactadherin-binding particles within the 0...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28167681/mir-450a-5p-within-rat-adipose-tissue-exosome-like-vesicles-promotes-adipogenic-differentiation-by-targeting-wisp2
#13
Yan Zhang, Mei Yu, Minjia Dai, Chang Chen, Qi Tang, Wei Jing, Hang Wang, Weidong Tian
Adipose tissue is an active endocrine organ that could secrete a wide number of factors to regulate adipogenesis via paracrine signals. In addition to soluble proteins in adipose tissue, microRNAs (miRNAs) enriched in extracellular vesicles (EVs), such as exosomes or microvesicles, could modulate intercellular communications. In this study, we demonstrated that exosome-like vesicles derived from adipose tissue (Exo-AT) were internalized by adipose tissue-derived stem cells (ADSCs), then induced adipogenesis...
February 6, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28167212/recruitment-of-rna-molecules-by-connexin-rna-binding-motifs-implication-in-rna-and-dna-transport-through-microvesicles-and-exosomes
#14
REVIEW
Marta Varela-Eirin, Adrian Varela-Vazquez, Marina Rodríguez-Candela Mateos, Anton Vila-Sanjurjo, Eduardo Fonseca, José L Mascareñas, M Eugenio Vázquez, Maria D Mayan
Connexins (Cxs) are integral membrane proteins that form high-conductance plasma membrane channels, allowing communication from cell to cell (via gap junctions) and from cells to the extracellular environment (via hemichannels). Initially described for their role in joining excitable cells (nerve and muscle), gap junctions (GJs) are found between virtually all cells in solid tissues and are essential for functional coordination by enabling the direct transfer of small signalling molecules, metabolites, ions, and electrical signals from cell to cell...
February 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28166726/endothelial-colony-forming-cells-ecfcs-in-murine-aki-implications-for-future-cell-based-therapies
#15
D Patschan, K Schwarze, B Tampe, M Zeisberg, S Patschan, G A Müller
BACKGROUND: In recent years, early Endothelial Progenitor Cells (eEPCs) have been proven as effective tool in murine ischemic AKI and in diabetic nephropathy. The mechanisms of eEPC-mediated vasoprotection have been elucidated in detail. Besides producing a diverse range of humoral factors, the cells also act by secreting vasomodulatory microvesicles. Only few data in contrast have been published about the role of so-called Endothelial Colony Forming Cells (ECFCs - late EPCs) in ischemic AKI...
February 6, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28161856/stem-cell-reviews-and-reports-microenvironment-and-extracellular-microvesicles-section
#16
EDITORIAL
Giovanni Camussi
No abstract text is available yet for this article.
February 4, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28153942/divergent-endothelial-function-but-similar-platelet-microvesicle-responses-following-eccentric-and-concentric-cycling-at-a-similar-aerobic-power-output
#17
Mark Rakobowchuk, Ophélie Ritter, Eurico Nestor Wilhelm, Laurie Isacco, Malika Bouhaddi, Bruno Degano, Nicolas Tordi, Laurent Mourot
Endothelial function and microvesicle concentration changes after acute bouts of continuous eccentric exercise have not been assessed previously nor compared to concentric exercise at similar aerobic power outputs. This method of training may be useful amongst some clinical populations, but acute responses are not well described. As such, twelve healthy males completed two experimental sessions of either 45 minutes of eccentric or concentric cycling at a matched aerobic power output below the ventilatory threshold...
February 2, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28151805/comparison-of-tissue-factor-expression-and-activity-in-foetal-and-adult-endothelial-cells
#18
Mary E W Collier, Atinuke Akinmolayan, Alison H Goodall
Tissue factor (TF) is not usually expressed by endothelial cells but can be induced in these cells by inflammatory cytokines. Many studies have used human umbilical vein endothelial cells (HUVEC) as a model to examine the regulation of TF expression. However, there is a question as to whether this reflects TF expression in adult endothelial cells. This study compared TF expression and the release of TF-positive microvesicles in HUVEC and adult human dermal blood endothelial cells (HDBEC) in response to tumour necrosis factor α (TNFα) and interleukin-1 β (IL-1β)...
February 1, 2017: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/28151481/epigenetic-regulation-of-hgf-met-receptor-axis-is-critical-for-the-outgrowth-of-bone-metastasis-from-breast-carcinoma
#19
Paola Bendinelli, Paola Maroni, Emanuela Matteucci, Maria Alfonsina Desiderio
Our translational research deals with the influence of microenvironment on the phenotype and colonization of bone metastases from breast carcinoma, and on pre-metastatic niche formation. The aim of the present study was to clarify the origin of hepatocyte growth factor (HGF), ligand of Met receptor, the control of the axis HGF/Met by DNA methylation, and its importance for the nexus supportive cells-metastatic cells and for metastasis outgrowth. In bone metastasis of the 1833-xenograft model, DNA methyltransferase blockade using the chemotherapic drug 5-aza-2'-deoxycytidine (decitabine) strongly reduced the expression of HGF/Met receptor axis and of E-cadherin, with decrease of metastasis wideness and osteolysis, prolonging mice survival...
February 2, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28150804/extracellular-vesicles-in-coronary-artery-disease
#20
REVIEW
Chantal M Boulanger, Xavier Loyer, Pierre-Emmanuel Rautou, Nicolas Amabile
Membrane vesicles released in the extracellular space are composed of a lipid bilayer enclosing soluble cytosolic material and nuclear components. Extracellular vesicles include apoptotic bodies, exosomes, and microvesicles (also known previously as microparticles). Originating from different subcellular compartments, the role of extracellular vesicles as regulators of transfer of biological information, acting locally and remotely, is now acknowledged. Circulating vesicles released from platelets, erythrocytes, leukocytes, and endothelial cells contain potential valuable biological information for biomarker discovery in primary and secondary prevention of coronary artery disease...
February 2, 2017: Nature Reviews. Cardiology
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