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J L Zheng, J Sun, H Zhang, Y Zhang
Both microRNA (miRNA) and long noncoding RNA (lncRNA) fall within the category of noncoding RNA. MiRNA is a 20-24 nt long, highly conserved, single-stranded noncoding RNA. MiRNA can specifically bind to the 3' untranslated region of target mRNA, induce the transcript degradation or translation inhibition, and eventually impact the biological functions of the cell, such as proliferation, differentiation, and apoptosis. Whereas lncRNA is an over 200 bp long, single-stranded, noncoding RNA, which can regulate the important biological processes, such as cell division, growth, differentiation and apoptosis...
May 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Xin Zhao, Yunxia Hou, Zhongzhen Tuo, Fangmeng Wei
The object of this study was to analyze the expression of miR-194 and miR-29 in gastric cancer and their roles in the regulation of malignant phenotype of gastric cancer cells, and to explore the application value of miR-194 and miR-29 in diagnosis and prognosis of gastric cancer. Tumor tissue and adjacent healthy tissue of 165 gastric cancer patients diagnosed by pathologic examinations were collected. Expression of miR-194 and miR-29 in the tissues was detected by RT-PCR. The relationship between miR-194 and miR-29 expression and clinical data was analyzed...
May 2018: Experimental and Therapeutic Medicine
Rong Wei, Rufang Zhang, Hongmei Li, Hongyun Li, Saiji Zhang, Yewei Xie, Shen Li, Fang Chen
We previously demonstrate that microRNAs (miRNA) play an important role in hypothermic circulatory arrest (DHCA)-associated neural injury. However, the specific role of miRNAs in the pathogenesis of DHCA-induced neuron death is still unclear. Thus, in the present study, we investigated miR-29 expression and roles in neuronal HT-22 cells with oxygen-glucose deprivation/reoxygenation (OGD/R). In this study, the model of OGD/R was established using an airtight culture container and the anaeropack. Measurement of reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) was used the probes of JC-1 and H2DCFDA...
April 3, 2018: Current Neurovascular Research
Jeremy Herrera, Daniel J Beisang, Mark Peterson, Colleen Forster, Adam Gilbertsen, Alexey Benyumov, Karen Smith, Christopher E Korenczuk, Victor H Barocas, Kacey Guenther, Ryan Hite, Lin Zhang, Craig A Henke, Peter B Bitterman
RATIONALE: The lung ECM in IPF mediates progression of fibrosis by decreasing fibroblast expression of microRNA-29 (miR-29), a master negative regulator of ECM production. The molecular mechanism is undefined. IPF ECM is stiffer than normal. Stiffness drives fibroblast ECM production in a YAP-dependent manner; and YAP is a known regulator of miR-29. Therefore, we tested the hypothesis that negative regulation of miR-29 by IPF ECM was mediated by mechanotransduction of stiffness. OBJECTIVES: To determine how IPF ECM negatively regulates miR-29...
March 26, 2018: American Journal of Respiratory and Critical Care Medicine
Tran T Hien, Eliana Garcia-Vaz, Karin G Stenkula, Johan Sjögren, Johan Nilsson, Maria F Gomez, Sebastian Albinsson
Diabetes is a major risk factor for cardiovascular disease and this is in part due to the effects of hyperglycemia on vascular smooth muscle cells. Small non-coding microRNAs are known to control smooth muscle phenotype and arterial contractility and are dysregulated in diabetes. The effect of microRNAs on smooth muscle differentiation is in part mediated by the transcription factor KLF4 but the role of this mechanism in diabetic vascular disease is not fully understood. Herein, we have investigated the importance of hyperglycemia and diabetes for the expression of KLF4 in vascular smooth muscle and the involvement of miRNAs in this regulation...
March 25, 2018: Journal of Cellular Physiology
Hongtao Hu, Shuang Hu, Shen Xu, Yue Gao, Fang Zeng, Hua Shui
Renal interstitial fibrosis is a necessary step in the progression of chronic kidney to end stage renal disease. MicroRNA-29 (miR-29) has been shown to play essential roles in epithelial-mesenchymal transition (EMT), and thus may contribute to the regulation of renal interstitial fibrosis. However, the role of miR-29 in the regulation of EMT during chronic kidney disease and renal transplantation has been a source of intense debate, and the mechanisms underlying this process are incompletely understood. In this study, we investigated the function of miR-29b in the regulation of EMT and to gain a better understanding of the mechanism by which miR-29b modulates EMT by targeting the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway during the process of renal interstitial fibrosis...
March 22, 2018: International Journal of Molecular Medicine
Tiago Pereira-da-Silva, Madalena Coutinho Cruz, Catarina Carrusca, Rui Cruz Ferreira, Patrícia Napoleão, Miguel Mota Carmo
AIMS: Atherosclerosis is associated with altered circulating microRNA profiles. It is yet unclear whether the expression of these potential biomarkers differs according to the location of atherosclerosis. We assessed whether atherosclerosis of different arterial territories, except the coronary, is associated with specific circulating microRNA profiles. METHODS: A systematic search in PubMed, Web of Science, Embase, and Cochrane Library was carried out using a retrieval strategy including MESH and non-MSH terms...
2018: American Journal of Cardiovascular Disease
Phimon Atsawasuwan, Paul Lazari, Yinghua Chen, Xiaofeng Zhou, Grace Viana, Carla A Evans
Secretory microRNAs (miRNAs) have been used increasingly as biomarkers for cancers, autoimmune diseases and inflammatory diseases. They are reported as being freely circulated or encapsulated in microvesicles such as exosomes. This study was performed to elucidate the presence of miRNAs with exosomes in human gingival crevicular fluid (GCF), and the expression profile of miRNA-29 during orthodontic tooth movement. Four healthy volunteer and fifteen orthodontic patients were enrolled in the study. Secretory miRNA in GCF was collected and analyzed using a bioanalyzer, realtime PCR and Western blot analysis...
2018: PloS One
Angelo Bitetti, Allison C Mallory, Elisabetta Golini, Claudia Carrieri, Héctor Carreño Gutiérrez, Emerald Perlas, Yuvia A Pérez-Rico, Glauco P Tocchini-Valentini, Anton J Enright, William H J Norton, Silvia Mandillo, Dónal O'Carroll, Alena Shkumatava
microRNAs (miRNAs) repress target transcripts through partial complementarity. By contrast, highly complementary miRNA-binding sites within viral and artificially engineered transcripts induce miRNA degradation in vitro and in cell lines. Here, we show that a genome-encoded transcript harboring a near-perfect and deeply conserved miRNA-binding site for miR-29 controls zebrafish and mouse behavior. This transcript originated in basal vertebrates as a long noncoding RNA (lncRNA) and evolved to the protein-coding gene NREP in mammals, where the miR-29-binding site is located within the 3' UTR...
March 2018: Nature Structural & Molecular Biology
Daria Domańska-Senderowska, Zbigniew Jastrzębski, Justyna Kiszałkiewicz, Michał Brzeziański, Dorota Pastuszak-Lewandoska, Łukasz Radzimińki, Ewa Brzeziańska-Lasota, Anna Jegier
Recently studies have shown that, depending on the type of training and its duration, the expression levels of selected circulating myomiRNAs (c-miR-27a,b, c-miR-29a,b,c, c-miR-133a) differ and correlate with the physiological indicators of adaptation to physical activity. To analyse the expression of selected classes of miRNAs in soccer players during different periods of their training cycle. The study involved 22 soccer players aged 17-18 years. The multi-stage 20-m shuttle run test was used to estimate VO2 max among the soccer players...
December 2017: Biology of Sport
Haiyan Song, Lei Ding, Shuang Zhang, Wei Wang
MicroRNA (miR)-29 family members have been reported to play important regulatory roles in metabolic disease. We used TargetScan to show that "secreted protein acidic rich in cysteine" (SPARC) is a target of the miR-29s. SPARC is a multifunctional secretory protein involved in a variety of biological activities, and SPARC dysregulation is associated with a wide range of obesity-related disorders, including type 2 diabetes mellitus (T2DM). We explored whether miR-29s played roles in glucose metabolism and whether miR-29s directly targeted SPARC...
March 4, 2018: Biochemical and Biophysical Research Communications
Apollinaire Ngankeu, Parvathi Ranganathan, Violaine Havelange, Deedra Nicolet, Stefano Volinia, Bayard L Powell, Jonathan E Kolitz, Geoffrey L Uy, Richard M Stone, Steven M Kornblau, Michael Andreeff, Carlo M Croce, Clara D Bloomfield, Ramiro Garzon
We previously reported that microRNA (miR)-29b is down-regulated and has a tumor suppressor role in acute myeloid leukemia (AML). However, little is known about the mechanisms responsible for miR-29b expression downregulation in AML. In this work we screened for mutations that could affect miR-29b expression. Using Sanger sequencing, we identified a germline thymidine (T) base deletion within the miR-29b-1/miR-29a cluster precursor in 16% of AML patients. Remarkably we found a significant enrichment for the presence of the miR-29 polymorphism in core binding factor (CBF) newly diagnosed AML patients ( n = 61/303; 20%) with respect to age, sex and race matched controls ( n = 43/402:11%, P < 0...
January 12, 2018: Oncotarget
Keiichi Koshizuka, Naoko Kikkawa, Toyoyuki Hanazawa, Yasutaka Yamada, Atsushi Okato, Takayuki Arai, Koji Katada, Yoshitaka Okamoto, Naohiko Seki
Due to their aggressive behavior, local recurrence and distant metastasis, survival rate of advanced stage of the patients with head and neck squamous cell carcinoma (HNSCC) is very poor. Currently available epidermal growth factor receptor (EGFR)-targeted therapies are not considered curative for HNSCC. Therefore, novel approaches for identification of therapeutic targets in HNSCC are needed. All members of the miRNA-29 family ( miR-29a , miR-29b , and miR-29c ) were downregulated in HNSCC tissues by analysis of RNA-sequencing based microRNA (miRNA) expression signature...
January 9, 2018: Oncotarget
Lei Cao, Yu Zhang, Shuai Zhang, Tian-Peng Jiang, Li Chen, Jing Liu, Shi Zhou
Cerebral ischemic injury causes severe brain damage and remains one of the leading causes of morbidity and mortality worldwide. Members of the microRNA-29 (miR-29) family are involved in regulating the process of ischemia and may be developed as biomarkers to diagnose and treat cerebral ischemia. The role of miR-29b in cerebral ischemia injury remains poorly understood. The purpose of the present study was to investigate whether miR-29b overexpression suppressed cerebral ischemic injury and to explore its underlying mechanism of action...
January 2018: Experimental and Therapeutic Medicine
Longxiang Tu, Qi Huang, Shangfeng Fu, Dewu Liu
A hypertrophic scar is the result of abnormal repair of the body after trauma. Histopathologically, it is mostly the result of the excessive proliferation of fibroblasts and the accumulation of extracellular matrix. Accumulating evidence has demonstrated that long non‑coding RNAs (lncRNAs) have a critical role in the regulation of gene expression and in the pathogenesis of diseases. However, the roles of lncRNAs in hypertrophic scars have remained elusive. The present study investigated the profiles of differentially expressed lncRNAs between fibroblasts derived from a hypertrophic scar and normal skin, and explored the possible mechanisms underlying the development of hypertrophic scars...
April 2018: International Journal of Molecular Medicine
Iana H Haralambieva, Richard B Kennedy, Whitney L Simon, Krista M Goergen, Diane E Grill, Inna G Ovsyannikova, Gregory A Poland
BACKGROUND: MicroRNAs are important mediators of post-transcriptional regulation of gene expression through RNA degradation and translational repression, and are emerging biomarkers of immune system activation/response after vaccination. METHODS: We performed Next Generation Sequencing (mRNA-Seq) of intracellular miRNAs in measles virus-stimulated B and CD4+ T cells from high and low antibody responders to measles vaccine. Negative binomial generalized estimating equation (GEE) models were used for miRNA assessment and the DIANA tool was used for gene/target prediction and pathway enrichment analysis...
2018: PloS One
Paweł Rubiś, Justyna Totoń-Żurańska, Sylwia Wiśniowska-Śmiałek, Ewa Dziewięcka, Maria Kołton-Wróż, Paweł Wołkow, Ewelina Pitera, Lucyna Rudnicka-Sosin, Ann C Garlitski, Andrzej Gackowski, Piotr Podolec
It is unknown whether fibrosis-associated microRNAs: miR-21, miR-26, miR-29, miR-30 and miR-133a are linked to cardiovascular (CV) outcome. The study evaluated the levels of extracellular matrix (ECM) fibrosis and the prevalence of particular microRNAs in patients with dilated cardiomyopathy (DCM) to investigate any correlation with CV events. METHODS: Seventy DCM patients (48 ± 12 years, EF 24.4 ± 7.4%) underwent right ventricular biopsy. The control group was comprised of 7 patients with CAD who underwent CABG and intraoperative biopsy...
April 2018: Journal of Cellular and Molecular Medicine
Eloïse A Kremer, Niharika Gaur, Melissa A Lee, Olivia Engmann, Johannes Bohacek, Isabelle M Mansuy
5-hydroxymethylation (5-hmC) is an epigenetic modification on DNA that results from the conversion of 5-methylcytosine by Ten-Eleven Translocation (TET) proteins. 5-hmC is widely present in the brain and is subjected to dynamic regulation during development and upon neuronal activity. It was recently shown to be involved in memory processes but currently, little is known about how it is controlled in the brain during memory formation. Here, we show that Tet3 is selectively up-regulated by activity in hippocampal neurons in vitro, and after formation of fear memory in the hippocampus...
January 26, 2018: Scientific Reports
Michael E Widlansky, David M Jensen, Jingli Wang, Yong Liu, Aron M Geurts, Alison J Kriegel, Pengyuan Liu, Rong Ying, Guangyuan Zhang, Marc Casati, Chen Chu, Mobin Malik, Amberly Branum, Michael J Tanner, Sudhi Tyagi, Kristie Usa, Mingyu Liang
We investigated the role of microRNAs (miRNA) in endothelial dysfunction in the setting of cardiometabolic disorders represented by type 2 diabetes mellitus (T2DM). miR-29 was dysregulated in resistance arterioles obtained by biopsy in T2DM patients. Intraluminal delivery of miR-29a-3p or miR-29b-3p mimics restored normal endothelium-dependent vasodilation (EDVD) in T2DM arterioles that otherwise exhibited impaired EDVD Intraluminal delivery of anti-miR-29b-3p in arterioles from non-DM human subjects or rats or targeted mutation of Mir29b-1/a gene in rats led to impaired EDVD and exacerbation of hypertension in the rats...
January 26, 2018: EMBO Molecular Medicine
Baoyi Zhu, Mari Ekman, Daniel Svensson, Jessica Lindvall, Bengt-Olof Nilsson, Bengt Uvelius, Karl Swärd
Bladder denervation and bladder outlet obstruction are urological conditions that cause bladder growth. Transcriptomic surveys in outlet obstruction have identified differentially expressed genes, but similar studies following denervation have not been done. This was addressed using a rat model in which the pelvic ganglia were cryo-ablated followed by bladder microarray analyses. At 10 days following denervation, bladder weight had increased 5.6-fold and 2890 mRNAs and 135 miRNAs were differentially expressed...
January 10, 2018: American Journal of Physiology. Renal Physiology
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