Rida Malik, Peng Liao, Ji Zhou, Rawaa Hussain, James Charles Fredenburgh, Lisa Hettrick, Alexey S Revenko, Jeffrey I Weitz
Factor (F) XII knockdown attenuates catheter thrombosis in rabbits. Because histidine-rich glycoprotein (HRG) modulates FXIIa activity, we hypothesized that HRG depletion would promote catheter thrombosis. To test this, rabbits were given antisense oligonucleotides (ASOs) against HRG, FXII, or a control ASO or saline. The activated partial thromboplastin time (aPTT), prothrombin time (PT), and catheter-induced thrombin generation were determined in blood collected before and after treatment. Compared with the controls, the HRG- and FXII-directed ASOs reduced hepatic mRNA and plasma levels of HRG and FXII, respectively, by over 90%...
April 12, 2023: Blood Advances