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https://www.readbyqxmd.com/read/29777036/pol%C3%AE-a-y-family-translesion-synthesis-polymerase-promotes-cellular-tolerance-of-myc-induced-replication-stress
#1
Kiminori Kurashima, Takayuki Sekimoto, Tsukasa Oda, Tsuyoshi Kawabata, Fumio Hanaoka, Takayuki Yamashita
Growth of precancerous and cancer cells relies on their tolerance of oncogene-induced replication stress (RS). Translesion synthesis (TLS) plays an essential role in cellular tolerance of various types of RS and bypasses replication barriers by employing specialized polymerases. However, limited information is available about the role of TLS polymerases in oncogene-induced RS. Here, we report that Polη, a Y-family TLS polymerase, promotes cellular tolerance of Myc-induced RS. Polη was recruited to Myc-induced RS sites, and Polη depletion enhanced the Myc-induced slowing and stalling of replication forks and the subsequent generation of double-strand breaks (DSBs)...
May 18, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29704992/opposing-roles-of-y-family-dna-polymerases-in-lipid-peroxide-mutagenesis-at-the-hisg46-target-in-the-ames-test
#2
Petr Grúz, Masatomi Shimizu, Masami Yamada, Kei-Ichi Sugiyama, Masamitsu Honma
DNA polymerases play a key role in mutagenesis by performing translesion DNA synthesis (TLS). The Y-family of DNA polymerases comprises several evolutionarily conserved families, specializing in TLS of different DNA adducts. Exocyclic etheno and propano DNA adducts are among the most common endogenous DNA lesions induced by lipid peroxidation reactions triggered by oxidative stress. We have investigated the participation of two enterobacterial representatives of the PolIV and PolV branches of Y-family DNA polymerases in mutagenesis by two model lipid peroxidation derived genotoxins, glyoxal and crotonaldehyde...
May 2018: Mutation Research
https://www.readbyqxmd.com/read/29683380/diverse-roles-of-rad18-and-y-family-dna-polymerases-in-tumorigenesis
#3
Yang Yang, Yanzhe Gao, Anastasia Zlatanou, Satoshi Tateishi, Vyacheslav Yurchenko, Igor B Rogozin, Cyrus Vaziri
Mutagenesis is a hallmark and enabling characteristic of cancer cells. The E3 ubiquitin ligase RAD18 and its downstream effectors, the 'Y-family' Trans-Lesion Synthesis (TLS) DNA polymerases, confer DNA damage tolerance at the expense of DNA replication fidelity. Thus, RAD18 and TLS polymerases are attractive candidate mediators of mutagenesis and carcinogenesis. The skin cancer-propensity disorder xeroderma pigmentosum-variant (XPV) is caused by defects in the Y-family DNA polymerase Pol eta (Polη). However it is unknown whether TLS dysfunction contributes more generally to other human cancers...
May 8, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29625036/coordinated-activity-of-y-family-tls-polymerases-and-exo1-protects-non-s-phase-cells-from-uv-induced-cytotoxic-lesions
#4
Sarah Sertic, Antonio Mollica, Ilaria Campus, Stefania Roma, Emanuela Tumini, Andrés Aguilera, Marco Muzi-Falconi
No abstract text is available yet for this article.
April 5, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29614052/genomic-characterization-of-sixteen-yersinia-enterocolitica-infecting-podoviruses-of-pig-origin
#5
Mabruka Salem, Mikael Skurnik
Yersinia enterocolitica causes enteric infections in humans and animals. Human infections are often caused by contaminated pork meat. Y. enterocolitica colonizes pig tonsils and pigs secrete both the human pathogen and its specific bacteriophages into the stools. In this work, sixteen Y. enterocolitica -infecting lytic bacteriophages isolated from pig stools originating from several pig farms were characterized. All phages belong to the Podoviridae family and their genomes range between 38,391-40,451 bp in size...
April 3, 2018: Viruses
https://www.readbyqxmd.com/read/29601523/genome-wide-analysis-of-dcl-ago-and-rdr-gene-families-in-pepper-capsicum-annuum-l
#6
Lei Qin, Ning Mo, Tayeb Muhammad, Yan Liang
RNA silencing is an evolutionarily conserved mechanism that regulates variety of cellular processes in plants. Argonaute protein (AGO), Dicer-like protein (DCL) and RNA-dependent RNA polymerase (RDR) are critical components of RNA silencing. These efficient and indispensable components of the RNAi pathway have not been identified and characterized in pepper. In this study, we identified 12 CaAGO , 4 CaDCL and 6 CaRDR genes in pepper and compared them with those of Arabidopsis, tobacco, potato and tomato. Detailed phylogenetic analyses revealed that each CaAGO, CaDCL and CaRDR protein family were classified into four clades...
March 30, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29557391/cyclin-y-modulates-the-proliferation-invasion-and-metastasis-of-hepatocellular-carcinoma-cells
#7
Kaishun Shi, Qingjing Ru, Chenyi Zhang, Jie Huang
BACKGROUND Cyclin Y (CCNY) is a member of the cyclin family of proteins that regulate the cell cycle. The aims of this study were to compare the expression of CCNY in normal liver and human hepatocellular carcinoma (HCC), in normal and HCC cell lines, and in mouse HCC tumor xenografts. MATERIAL AND METHODS Tumor tissues from 55 patients diagnosed with HCC were studied for CCNY expression. Human HCC cell lines, SK-Hep1, HepG2, HEP3B, HuH7 and L02 were studied using the MTT cell proliferation assay, cell apoptosis, transwell and wound healing assays...
March 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29551515/coordinated-activity-of-y-family-tls-polymerases-and-exo1-protects-non-s-phase-cells-from-uv-induced-cytotoxic-lesions
#8
Sarah Sertic, Antonio Mollica, Ilaria Campus, Stefania Roma, Emanuela Tumini, Andrés Aguilera, Marco Muzi-Falconi
UV-induced photoproducts are responsible for the pathological effects of sunlight. Mutations in nucleotide excision repair (NER) cause severe pathologies characterized by sunlight sensitivity, coupled to elevated predisposition to cancer and/or neurological dysfunctions. We have previously shown that in UV-irradiated non-cycling cells, only a particular subset of lesions activates the DNA damage response (DDR), and this requires NER and EXO1 activities. To define the molecular mechanism acting at these lesions, we demonstrate that Y family TLS polymerases are recruited at NER- and EXO1-positive lesion sites in non-S phase cells...
April 5, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29404532/y-chromosome-microdeletions-frequency-in-idiopathic-azoospermia-oligoasthenozoospermia-and-oligospermia
#9
Delnya Gholami, Hamideh Jafari-Ghahfarokhi, Maryam Nemati-Dehkordi, Hossien Teimori
Background: Genetic factors are candidates for about 30% of male infertility with sperm production-related abnormalities. Y chromosome microdeletions are responsible for around 10% of male infertility. These microdeletions generally occur in azoospermia factor on the Yq. That is often associated with the quantitative reduction of sperm. Objective: The aim of this cross-sectional study was to determine the frequency of Yq microdeletions among idiopathic azoospermic, oligoasthenozoospermic, and oligospermic men in Shohada infertility center, Chaharmahal va Bakhtiari province...
November 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29363281/detection-and-genetic-characterization-of-porcine-deltacoronavirus-in-tibetan-pigs-surrounding-the-qinghai-tibet-plateau-of-china
#10
M Wang, Y Wang, A R Baloch, Y Pan, L Tian, F Xu, S Shivaramu, S Chen, Q Zeng
Porcine deltacoronavirus (PDCoV) is a recently discovered RNA virus that belongs to the family Coronaviridae and genus Deltacoronavirus. This virus causes enteric disease in piglets that is characterized by enteritis and diarrhoea. In our present investigation, 189 diarrhoeic samples were collected between July 2016 and May 2017 from Tibetan pigs inhabiting in three different provinces surrounding the Qinghai-Tibet Plateau of China. We then applied the molecular-based method of reverse transcription polymerase chain reactions (RT-PCRs) to detect the presence of PDCoV in collected samples, and RT-PCR indicated that the prevalence of PDCoV was 3...
April 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29318463/a-novel-splice-site-mutation-of-the-prkar1a-gene-c-440-5-g-c-in-a-chinese-family-with-carney-complex
#11
J Fu, F Lai, Y Chen, X Wan, G Wei, Y Li, H Xiao, X Cao
BACKGROUND: Carney complex (CNC) is an extremely rare, multiple endocrine neoplasia syndrome that occurs in an autosomal dominant manner. Mutations in PRKAR1A have been reported to be a common genetic cause of CNC. METHODS: In this study, we reported a Chinese pedigree of CNC that manifests mainly as spotty skin pigmentation and primary pigmented nodular adrenocortical disease. Whole blood samples of this pedigree were collected for DNA/RNA analysis. Polymerase chain reaction (PCR) and reverse-transcription polymerase chain reaction analyses were performed to amplify the 11 exons and adjacent introns of PRKAR1A...
January 9, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29284754/viral-discovery-and-diversity-in-trypanosomatid-protozoa-with-a-focus-on-relatives-of-the-human-parasite-leishmania
#12
Danyil Grybchuk, Natalia S Akopyants, Alexei Y Kostygov, Aleksandras Konovalovas, Lon-Fye Lye, Deborah E Dobson, Haroun Zangger, Nicolas Fasel, Anzhelika Butenko, Alexander O Frolov, Jan Votýpka, Claudia M d'Avila-Levy, Pavel Kulich, Jana Moravcová, Pavel Plevka, Igor B Rogozin, Saulius Serva, Julius Lukeš, Stephen M Beverley, Vyacheslav Yurchenko
Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania ( Crithidia and Leptomonas ), as well as plant-infecting Phytomonas Leptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization...
January 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29247828/hypersensitivity-of-mouse-embryonic-fibroblast-cells-defective-for-dna-polymerases-%C3%AE-%C3%AE-and-%C3%AE%C2%BA-to-various-genotoxic-compounds-its-potential-for-application-in-chemical-genotoxic-screening
#13
Junichi Akagi, Masayuki Yokoi, Young-Man Cho, Takeshi Toyoda, Haruo Ohmori, Fumio Hanaoka, Kumiko Ogawa
Genotoxic agents cause modifications of genomic DNA, such as alkylation, oxidation, bulky adduct formation, and strand breaks, which potentially induce mutations and changes to the structure or number of genes. Majority of point mutations are generated during error-prone bypass of modified nucleotides (translesion DNA synthesis, TLS); however, when TLS fails, replication forks stalled at lesions eventually result in more lethal effects, formation of double-stranded breaks (DSBs). Here we compared sensitivities to various compounds among mouse embryonic fibroblasts derived from wild-type and knock-out mice lacking one of the three Y-family TLS DNA polymerases (Polη, Polι, and Polκ) or all of them (TKO)...
January 2018: DNA Repair
https://www.readbyqxmd.com/read/29238954/interleukin-25-is-involved-in-cutaneous-t-cell-lymphoma-progression-by-establishing-a-t-helper-2-dominant-microenvironment
#14
R Nakajima, T Miyagaki, M Hirakawa, T Oka, N Takahashi, H Suga, A Yoshizaki, H Fujita, Y Asano, M Sugaya, S Sato
BACKGROUND: Interleukin (IL)-25 is a member of the IL-17 family, which can promote and augment T-helper (Th) type 2 responses. The expression of IL-25 and its cognate receptor, IL-25 receptor (IL-25R), is upregulated and correlated with disease activity in Th2-associated diseases. OBJECTIVES: To examine the expression and function of IL-25 in cutaneous T-cell lymphoma (CTCL). METHODS: Expression and location of IL-25 in lesional skin was investigated with immunohistochemistry...
December 13, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/29234107/bulky-lesion-bypass-requires-dpo4-binding-in-distinct-conformations
#15
Pramodha S Liyanage, Alice R Walker, Alfonso Brenlla, G Andrés Cisneros, Louis J Romano, David Rueda
Translesion DNA synthesis is an essential process that helps resume DNA replication at forks stalled near bulky adducts on the DNA. Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon (PAH) that can be metabolically activated to benzo[a]pyrene diol epoxide (BPDE), which then can react with DNA to form carcinogenic DNA adducts. Here, we have used single-molecule florescence resonance energy transfer (smFRET) experiments, classical molecular dynamics simulations, and nucleotide incorporation assays to investigate the mechanism by which the model Y-family polymerase, Dpo4, bypasses a (+)-cis-B[a]P-N2 -dG adduct in DNA...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29224322/-%C3%AE-1-antitrypsin-pittsburg-mutations-report-of-two-cases-in-the-same-family
#16
L J Cao, X Bai, Z Q Yu, W Zhang, J Su, Z Y Wang, C G Ruan
Objective: To analyze the clinical and laboratory abnormalities of two patients with α1-antitrypsin (α1-AT) Pittsburgh in a family and review the literatures. Methods: Both plasma clotting time and factor activities were performed using clotting or substrate methods. Platelet aggregation was evaluated using an optical aggregometer. The serum protein electrophoresis was performed on Sebia HYDRASYS by using Agarose gel. The exons of α1-AT were amplified by using polymerase chain reaction (PCR) and then sequenced and compared with NCBI GenBank records...
November 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29198202/effects-of-insecticides-chlorpyrifos-emamectin-benzoate-and-fipronil-on-spodoptera-litura-might-be-mediated-by-obps-and-csps
#17
X Lin, Y Jiang, L Zhang, Y Cai
Spodoptera litura is a widespread polyphagous insect pest that can develop resistance and cross-resistance to insecticides, making it difficult to control. Insecticide exposure has previously been linked with induction of specific olfactory-related proteins, including some chemosensory proteins (CSPs) and odorant-binding proteins (OPBs), which may disrupt detection of environmental factors and reduce fitness. However, functional evidence supporting insecticide and OBPs/CSPs mediation remains unknown. Here we fed male S...
December 4, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29197589/sirt6-expression-and-oxidative-dna-damage-in-individuals-with-prediabetes-and-type-2-diabetes-mellitus
#18
Z Çalışkan, T Mutlu, M Güven, M Tunçdemir, M Niyazioğlu, Y Hacioglu, Y Dincer
Sirtuins (SIRTs) is a family of NAD+ dependent histone deacetylases. SIRT6 takes play in glucose homeostasis, genomic stability and DNA repair. Although increased oxidative DNA damage and decreased DNA repair activity were determined in diabetes mellitus, the possible relation between level of oxidative DNA damage and SIRT6 expression has not been investigated so far. We determined SIRT6 expression and urinary 8-hydroxy deoxyguanosine (8-OHdG) levels, marker of oxidative DNA damage, in cases with prediabetes (PreDM) and type 2 diabetes mellitus (T2DM)...
February 5, 2018: Gene
https://www.readbyqxmd.com/read/29129138/the-electrostatic-role-of-the-zn-cys2his2-complex-in-binding-of-operator-dna-with-transcription-factors-mouse-egr-1-from-the-cys2his2-family
#19
Y N Chirgadze, E A Boshkova, R V Polozov, V S Sivozhelezov, A V Dzyabchenko, M B Kuzminsky, V A Stepanenko, V V Ivanov
The mouse factor Zif268, known also as early growth response protein EGR-1, is a classical representative for the Cys2His2 transcription factor family. It is required for binding the RNA polymerase with operator dsDNA to initialize the transcription process. We have shown that only in this family of total six Zn-finger protein families the Zn complex plays a significant role in the protein-DNA binding. Electrostatic feature of this complex in the binding of factor Zif268 from Mus musculus with operator DNA has been considered...
January 7, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29116069/-participation-of-the-pirna-pathway-in-recruiting-a-component-of-rna-polymerase-i-transcription-initiation-complex-to-germline-cell-nucleoli
#20
E A Fefelova, A D Stolyarenko, E Y Yakushev, V A Gvozdev, M S Klenov
Proteins of the Piwi family and short Piwi-interacting RNAs (piRNAs) ensure the protection of the genome from transposable elements. We have previously shown that nuclear Piwi protein tends to concentrate in the nucleoli of the cells of Drosophila melanogaster ovaries. It could be hypothesized that the function of Piwi in the nucleolus is associated with the repression of R1 and R2 retrotransposons inserted into the rDNA cluster. Here, we show that Piwi participates in recruiting Udd protein to nucleoli. Udd is a component of the conserved Selectivity Factor I-like (SL1-like) complex, which is required for transcription initiation by RNA polymerase I...
September 2017: Molekuliarnaia Biologiia
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