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https://www.readbyqxmd.com/read/29124841/regulation-of-cysteine-residues-in-lsrb-proteins-from-sinorhizobium-meliloti-under-free-living-and-symbiotic-oxidative-stress
#1
Guirong Tang, Shenghui Xing, Sunjun Wang, Liangliang Yu, Xuan Li, Christian Staehelin, Menghua Yang, Li Luo
The development of legume nitrogen-fixing nodules is regulated by reactive oxygen species (ROS) produced by symbionts. Several regulators from Rhizobium are involved in ROS sensing. In a previous study, we found that Sinorhizobium meliloti LsrB regulates lipopolysaccharide production and is associated with H2 O2 accumulation in alfalfa (Medicago sativa) nodules. However, its underlying regulatory mechanism remains unclear. Here, we report that the cysteine residues in LsrB are required for adaptation to oxidative stress, gene expression, alfalfa nodulation and nitrogen fixation...
November 10, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29121239/overproduction-of-s-meliloti-argc-n-acetyl-gamma-glutamyl-phosphate-reductase-promotes-growth-delay-and-inefficient-nodules
#2
Carmen Vargas-Lagunas, Yolanda Mora, Rafael Díaz, Gabriel Martínez-Batallar, Lourdes Girard, Sergio Encarnación, Humberto Peralta, Jaime Mora
argC encodes N-acetyl-gamma-glutamyl phosphate reductase, the enzyme that catalyzes the high-energy consuming third step in the arginine synthesis pathway. A comparative analysis revealed two translation start sites in argC from Sinorhizobium meliloti. To determine whether both protein versions are synthesized in the organism and their functional role, we obtained genetic constructs with one (1S) or two (2S) start sites, with promoters of low (pspeB) or high (plac) transcriptional rate. The constructs were transferred to the S...
November 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29104370/is-there-sufficient-ensifer-and-rhizobium-species-diversity-in-uk-farmland-soils-to-support-red-clover-trifolium-pratense-white-clover-t-repens-lucerne-medicago-sativa-and-black-medic-m-lupulina
#3
Rachel Roberts, Robert W Jackson, Tim H Mauchline, Penny R Hirsch, Liz J Shaw, Thomas F Döring, Hannah E Jones
Rhizobia play important roles in agriculture owing to their ability to fix nitrogen through a symbiosis with legumes. The specificity of rhizobia-legume associations means that underused legume species may depend on seed inoculation with their rhizobial partners. For black medic (Medicago lupulina) and lucerne (Medicago sativa) little is known about the natural prevalence of their rhizobial partner Ensifer meliloti in UK soils, so that the need for inoculating them is unclear. We analysed the site-dependence of rhizobial seed inoculation effects on the subsequent ability of rhizobial communities to form symbioses with four legume species (Medicago lupulina, M...
November 2017: Applied Soil Ecology: a Section of Agriculture, Ecosystems & Environment
https://www.readbyqxmd.com/read/29076560/an-account-of-evolutionary-specialization-the-abcr-small-rnas-in-the-rhizobiales
#4
REVIEW
Lauren M Sheehan, Clayton C Caswell
The AbcR small RNAs (sRNAs) are a fascinating example of two highly conserved sRNAs that differ tremendously at the functional level amongst organisms. From their transcriptional activation to their regulatory capabilities, the AbcR sRNAs exhibit varying characteristics in three well-studied bacteria belonging to the Rhizobiales order: the plant symbiont Sinorhizobium meliloti, the plant pathogen Agrobacterium tumefaciens, and the animal pathogen Brucella abortus. This review outlines the similarities and differences of the AbcR sRNAs between each of these organisms, and discusses reasons as to why this group of sRNAs has diverged in their genetic organization and regulatory functions across species...
October 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29051066/study-of-the-cys-his-bridge-electron-transfer-pathway-in-a-copper-containing-nitrite-reductase-by-site-directed-mutagenesis-spectroscopic-and-computational-methods
#5
Julio C Cristaldi, María C Gómez, Pablo J González, Felix M Ferroni, Sergio Dalosto, Alberto C Rizzi, María G Rivas, Carlos D Brondino
The Cys-His bridge as electron transfer conduit in the enzymatic catalysis of nitrite to nitric oxide by nitrite reductase from Sinorhizobium meliloti 2011 (SmNir) was evaluated by site-directed mutagenesis, steady state kinetic studies, UV-vis and EPR spectroscopic measurements as well as computational calculations. The kinetic, structural and spectroscopic properties of the His171Asp (H171D) and Cys172Asp (C172D) SmNir variants were compared with the wild type enzyme. Molecular properties of H171D and C172D indicate that these point mutations have not visible effects on the quaternary structure of SmNir...
October 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29050878/stable-symbiotic-nitrogen-fixation-under-water-deficit-field-conditions-by-a-stress-tolerant-alfalfa-microsymbiont-and-its-complete-genome-sequence
#6
Cintia Jozefkowicz, Silvina Brambilla, Romina Frare, Margarita Stritzler, Carlos Piccinetti, Mariana Puente, Carolina Andrea Berini, Pedro Reyes Pérez, Gabriela Soto, Nicolás Ayub
We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine in isolated bacteroids positively correlated with low precipitation levels, suggesting that this compound acts as a critical osmoprotectant under field conditions. Genome analysis revealed that strain B401 contains alternative pathways for the biosynthesis and uptake of glycine betaine and its precursors...
October 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29049366/transgenic-expression-of-antimicrobial-peptide-d2a21-confers-resistance-to-diseases-incited-by-pseudomonas-syringae-pv-tabaci-and-xanthomonas-citri-but-not-candidatus-liberibacter-asiaticus
#7
Guixia Hao, Shujian Zhang, Ed Stover
Citrus Huanglongbing (HLB) associated with 'Candidatus Liberibacter asiaticus' (Las) and citrus canker disease incited by Xanthomonas citri are the most devastating citrus diseases worldwide. To control citrus HLB and canker disease, we previously screened over forty antimicrobial peptides (AMPs) in vitro for their potential application in genetic engineering. D2A21 was one of the most active AMPs against X. citri, Agrobacterium tumefaciens and Sinorhizobium meliloti with low hemolysis activity. Therefore, we conducted this work to assess transgenic expression of D2A21 peptide to achieve citrus resistant to canker and HLB...
2017: PloS One
https://www.readbyqxmd.com/read/28990486/nodule-specific-cysteine-rich-peptides-negatively-regulate-nitrogen-fixing-symbiosis-in-a-strain-specific-manner-in-medicago-truncatula
#8
Qi Wang, Jinge Liu, Hua Li, Shengming Yang, Peter Körmöczi, Attila Kereszt, Hongyan Zhu
Medicago truncatula shows a high level of specificity when interacting with its symbiotic partner Sinorhizobium meliloti. This specificity is mainly manifested at the nitrogen-fixing stage of nodule development, such that a particular bacterial strain forms nitrogen-fixing nodules (Nod+/Fix+) on one plant genotype but ineffective nodules (Nod+/Fix-) on another. Recent studies have just begun to reveal the underlying molecular mechanisms that control this specificity. The S. meliloti strain A145 induces the formation of Fix+ nodules on the accession DZA315...
October 9, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28978203/silver-engineered-nanomaterials-and-ions-elicit-species-specific-o2-consumption-responses-in-plant-growth-promoting-rhizobacteria
#9
Ricky W Lewis, Jason Unrine, Paul M Bertsch, David H McNear
Metal containing engineered nanomaterials (ENMs) are now commonly used in various industrial and commercial applications. Many of these materials can be transformed during waste water treatment and ultimately enter terrestrial ecosystems via agriculturally applied biosolids. It is unclear how agriculturally important soil microbes will be affected by exposure to environmentally relevant, sublethal concentrations of ENMs and their transformation products (i.e., ions, aggregates, etc.). A method was developed, which puts O2 consumption responses in terms of viability, and tested by examining the toxic effects of Ag(+), Zn(2+), and Ni(2+) ions on the plant growth promoting rhizobacterium (PGPR) Bacillus amyloliquefaciens GB03...
October 4, 2017: Biointerphases
https://www.readbyqxmd.com/read/28975015/high-quality-draft-genome-sequence-of-ensifer-meliloti-mlalz-1-a-microsymbiont-of-medicago-laciniata-l-miller-collected-in-lanzarote-canary-islands-spain
#10
Wan Adnawani Meor Osman, Peter van Berkum, Milagros León-Barrios, Encarna Velázquez, Patrick Elia, Rui Tian, Julie Ardley, Margaret Gollagher, Rekha Seshadri, T B K Reddy, Natalia Ivanova, Tanja Woyke, Amrita Pati, Victor Markowitz, Mohamed N Baeshen, Naseebh Nabeeh Baeshen, Nikos Kyrpides, Wayne Reeve
10.1601/nm.1335 Mlalz-1 (INSDC = ATZD00000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing nodule of Medicago laciniata (L.) Miller from a soil sample collected near the town of Guatiza on the island of Lanzarote, the Canary Islands, Spain. This strain nodulates and forms an effective symbiosis with the highly specific host M. laciniata. This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) sequencing project...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28916561/succinate-transport-is-not-essential-for-symbiotic-nitrogen-fixation-by-sinorhizobium-meliloti-nor-rhizobium-leguminosarum
#11
Michael J Mitsch, George C diCenzo, Alison Cowie, Turlough M Finan
Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria during endosymbiotic relationships with plants. The bacteria require the plant to provide a carbon source for generation of reductant to power SNF. While C4-dicarboxylates (succinate, fumarate, malate) appear to be the primary, if not sole, carbon source provided to the bacteria, the contribution of each C4-dicarboxylate is not known. We address this issue using a genetic and systems-level analyses. Expression of a malate specific transporter (MaeP) in Sinorhizobium meliloti Rm1021 dct mutants unable to transport C4-dicarboxylates resulted in malate import rates up to 30% that of wild type...
September 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28905086/identification-and-characterization-of-a-novel-vibo-quercitol-1-dehydrogenase-from-burkholderia-terrae-suitable-for-production-of-vibo-quercitol-from-2-deoxy-scyllo-inosose
#12
Nobuya Itoh, Junji Kurokawa, Hiroshi Toda, Kazunobu Konishi
(-)-vibo-Quercitol is a deoxyinositol (1L-1,2,4/3,5-cyclohexanepentol) that naturally occurs in oak species, honeydew honey, wines aged in oak barrels, and Gymnema sylvestre and is a potential intermediate for pharmaceuticals. We found that (-)-vibo-quercitol is stereoselectively synthesized from 2-deoxy-scyllo-inosose by the reductive reaction of a novel (-)-vibo-quercitol 1-dehydrogenase found in the proteomes of Burkholderia, Pseudomonas, and Arthrobacter. Among them, Burkholderia terrae sp. AKC-020 (40-1) produced a (-)-vibo-quercitol 1-dehydrogenase appropriate for synthesizing (-)-vibo-quercitol with a high diastereomeric excess...
October 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28905000/transcriptome-analysis-of-polyhydroxybutyrate-cycle-mutants-reveals-discrete-loci-connecting-nitrogen-utilization-and-carbon-storage-in-sinorhizobium-meliloti
#13
Maya D'Alessio, Ricardo Nordeste, Andrew C Doxey, Trevor C Charles
Polyhydroxybutyrate (PHB) and glycogen polymers are produced by bacteria as carbon storage compounds under unbalanced growth conditions. To gain insights into the transcriptional mechanisms controlling carbon storage in Sinorhizobium meliloti, we investigated the global transcriptomic response to the genetic disruption of key genes in PHB synthesis and degradation and in glycogen synthesis. Under both nitrogen-limited and balanced growth conditions, transcriptomic analysis was performed with genetic mutants deficient in PHB synthesis (phbA, phbB, phbAB, and phbC), PHB degradation (bdhA, phaZ, and acsA2), and glycogen synthesis (glgA1)...
September 2017: MSystems
https://www.readbyqxmd.com/read/28871850/succinoglycan-production-contributes-to-acidic-ph-tolerance-in-sinorhizobium-meliloti-rm1021
#14
Justin P Hawkins, Barney A Geddes, Ivan J Oresnik
In this work, the hypothesis that exopolysaccharide plays a role in the survival of Sinorhizobium meliloti at low pH levels is addressed. When S. meliloti was grown at pH 5.75, synthesis of succinoglycan increased, whereas synthesis of galactoglucan decreased. Succinoglycan that was isolated from cultures grown at low pH had a lower degree of polymerization relative to that which was isolated from cultures grown at neutral pH, suggesting that low-molecular weight (LMW) succinoglycan might play a role in adaptation to low pH...
December 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28851745/a-key-regulator-of-the-glycolytic-and-gluconeogenic-central-metabolic-pathways-in-sinorhizobium-meliloti
#15
George C diCenzo, Zahed Muhammed, Magne Østerås, Shelley A P O'Brien, Turlough M Finan
The order Rhizobiales contains numerous agriculturally, biotechnologically, and medically important bacteria, including the rhizobia, and the genera Agrobacterium, Brucella, and Methylobacterium, among others. These organisms tend to be metabolically versatile, but there has been relatively little investigation into the regulation of their central carbon metabolic pathways. Here, RNA-sequencing and promoter fusion data are presented to show that the PckR protein is a key regulator of central carbon metabolism in Sinorhizobium meliloti; during growth with gluconeogenic substrates, PckR represses expression of the complete Entner-Doudoroff glycolytic pathway and induces expression of the pckA and fbaB gluconeogenic genes...
November 2017: Genetics
https://www.readbyqxmd.com/read/28842338/structure-proteome-and-genome-of-sinorhizobium-meliloti-phage-%C3%AE-m5-a-virus-with-luz24-like-morphology-and-a-highly-mosaic-genome
#16
Matthew C Johnson, Marta Sena-Velez, Brian K Washburn, Georgia N Platt, Stephen Lu, Tess E Brewer, Jason S Lynn, M Elizabeth Stroupe, Kathryn M Jones
Bacteriophages of nitrogen-fixing rhizobial bacteria are revealing a wealth of novel structures, diverse enzyme combinations and genomic features. Here we report the cryo-EM structure of the phage capsid at 4.9-5.7 Å-resolution, the phage particle proteome, and the genome of the Sinorhizobium meliloti-infecting Podovirus ΦM5. This is the first structure of a phage with a capsid and capsid-associated structural proteins related to those of the LUZ24-like viruses that infect Pseudomonas aeruginosa. Like many other Podoviruses, ΦM5 is a T=7 icosahedron with a smooth capsid and short, relatively featureless tail...
August 22, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28827694/a-novel-c-di-gmp-binding-domain-in-glycosyltransferase-bgsa-is-responsible-for-the-synthesis-of-a-mixed-linkage-%C3%AE-glucan
#17
Daniel Pérez-Mendoza, Daniela Bertinetti, Robin Lorenz, María-Trinidad Gallegos, Friedrich W Herberg, Juan Sanjuán
BgsA is the glycosyltransferase (GT) involved in the synthesis of a linear mixed-linkage β-glucan (MLG), a recently described exopolysaccharide activated by c-di-GMP in Sinorhizobium meliloti and other Rhizobiales. Although BgsA displays sequence and structural homology with bacterial cellulose synthases (CS), it does not contain any predictable c-di-GMP binding domain. In this work we demonstrate that the cytoplasmic C-terminal domain of BgsA (C-BgsA) binds c-di-GMP with both high affinity (KD = 0.23 μM) and specificity...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805544/rna-silencing-in-plant-symbiotic-bacteria-insights-from-a-protein-centric-view
#18
José I Jiménez-Zurdo, Marta Robledo
Extensive work in model enterobacteria has evidenced that the RNA chaperone Hfq and several endoribonucleases, such as RNase E or RNase III, serve pivotal roles in small RNA-mediated post-transcriptional silencing of gene expression. Characterization of these protein hubs commonly provide global functional and mechanistic insights into complex sRNA regulatory networks. The legume endosymbiont Sinorhizobium meliloti is a non-classical model bacterium with a very complex lifestyle in which riboregulation is expected to play important adaptive functions...
August 14, 2017: RNA Biology
https://www.readbyqxmd.com/read/28792680/transcriptomic-basis-of-genome-by-genome-variation-in-a-legume-rhizobia-mutualism
#19
Liana T Burghardt, Joseph Guhlin, Chan Lan Chun, Junqi Liu, Michael J Sadowsky, Robert M Stupar, Nevin D Young, Peter Tiffin
In the legume-rhizobia mutualism, the benefit each partner derives from the other depends on the genetic identity of both host and rhizobial symbiont. To gain insight into the extent of genome × genome interactions on hosts at the molecular level and to identify potential mechanisms responsible for the variation, we examined host gene expression within nodules (the plant organ where the symbiosis occurs) of four genotypes of Medicago truncatula grown with either Ensifer meliloti or E. medicae symbionts...
August 9, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28768212/effects-of-biosolids-from-a-wastewater-treatment-plant-receiving-manufactured-nanomaterials-on-medicago-truncatula-and-associated-soil-microbial-communities-at-low-nanomaterial-concentrations
#20
Chun Chen, Olga V Tsyusko, Dave H McNear, Jonathan Judy, Ricky W Lewis, Jason M Unrine
Concern has grown regarding engineered nanomaterials (ENMs) entering agricultural soils through the application of biosolids and their possible effects on agroecosystems, even though the ENMs are extensively transformed. The effects of exposure to biosolids containing transformation products of these ENMs at low concentrations remain largely unexplored. We examined the responses of Medicago truncatula and its symbiotic rhizobia Sinorhizobium meliloti exposed to soil amended with biosolids from WWTP containing low added concentrations of ENMs (ENM Low), bulk/dissolved metals (bulk/dissolved Low), or no metal additions (control)...
December 31, 2017: Science of the Total Environment
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