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https://www.readbyqxmd.com/read/28713341/prevalence-host-range-and-comparative-genomic-analysis-of-temperate-ochrobactrum-phages
#1
Claudia Jäckel, Stefan Hertwig, Holger C Scholz, Karsten Nöckler, Jochen Reetz, Jens A Hammerl
Ochrobactrum and Brucella are closely related bacteria that populate different habitats and differ in their pathogenic properties. Only little is known about mobile genetic elements in these genera which might be important for survival and virulence. Previous studies on Brucella lysogeny indicated that active phages are rare in this genus. To gain insight into the presence and nature of prophages in Ochrobactrum, temperate phages were isolated from various species and characterized in detail. In silico analyses disclosed numerous prophages in published Ochrobactrum genomes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28686606/plant-fed-versus-chemicals-fed-rhizobacteria-of-lucerne-plant-only-teabags-culture-media-not-only-increase-culturability-of-rhizobacteria-but-also-recover-a-previously-uncultured-lysobacter-sp-novosphingobium-sp-and-pedobacter-sp
#2
Nabil A Hegazi, Mohamed S Sarhan, Mohamed Fayez, Sascha Patz, Brian R Murphy, Silke Ruppel
In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard nutrient agar, where we found that the cultivable populations significantly increased by up to 60% of the total bacterial numbers as estimated by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR)...
2017: PloS One
https://www.readbyqxmd.com/read/28649348/no-evidence-for-adaptation-to-local-rhizobial-mutualists-in-the-legume-medicago-lupulina
#3
Tia L Harrison, Corlett W Wood, Isabela L Borges, John R Stinchcombe
Local adaptation is a common but not ubiquitous feature of species interactions, and understanding the circumstances under which it evolves illuminates the factors that influence adaptive population divergence. Antagonistic species interactions dominate the local adaptation literature relative to mutualistic ones, preventing an overall assessment of adaptation within interspecific interactions. Here, we tested whether the legume Medicago lupulina is adapted to the locally abundant species of mutualistic nitrogen-fixing rhizobial bacteria that vary in frequency across its eastern North American range...
June 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28625986/the-irr-and-rira-proteins-participate-in-a-complex-regulatory-circuit-and-act-in-concert-to-modulate-bacterioferritin-expression-in-ensifer-meliloti-1021
#4
Daniela Costa, Vanesa Amarelle, Claudio Valverde, Mark R O'Brian, Elena Fabiano
In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and oxidative stress response. This gene is located downstream and overlapping the smc03787 ORF. No well predicted RirA or Irr boxes were found in the region immediately upstream the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787 We demonstrate that bfr gene expression is enhanced under iron sufficient condition and that Irr and RirA modulate this expression...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28618121/specificity-traits-consistent-with-legume-rhizobia-coevolution-displayed-by-ensifer-meliloti-rhizosphere-colonization
#5
María Eugenia Salas, Mauricio Javier Lozano, José Luis López, Walter Draghi, Javier Serrania, Gonzalo Arturo Torres Tejerizo, Francisco Javier Albicoro, Juliet Fernanda Nilsson, Mariano Pistorio, María Florencia Del Papa, Gustavo Parisi, Anke Becker, Antonio Lagares
Rhizobia are α- and ß-Proteobacteria that associate with legumes in symbiosis to fix atmospheric nitrogen. The chemical communication between roots and rhizobia begins in the rhizosphere. Using signature-tagged-Tn5 mutagenesis (STM) we performed a genome-wide screening for Ensifer meliloti genes that participate in colonizing the rhizospheres of alfalfa and other legumes. The analysis of ca. 6,000 mutants indicated that genes relevant for rhizosphere colonization account for nearly 2% of the rhizobial genome and that most (ca...
June 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28611764/interplay-of-pathogen-induced-defense-responses-and-symbiotic-establishment-in-medicago-truncatula
#6
Tao Chen, Liujian Duan, Bo Zhou, Haixiang Yu, Hui Zhu, Yangrong Cao, Zhongming Zhang
Suppression of host innate immunity appears to be required for the establishment of symbiosis between rhizobia and host plants. In this study, we established a system that included a host plant, a bacterial pathogen and a symbiotic rhizobium to study the role of innate immunity during symbiotic interactions. A pathogenic bacterium, Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000), was shown to cause chlorosis in Medicago truncatula A17. Sinorhizobium meliloti strain Sm2011 (Sm2011) and Pst DC3000 strain alone induced similar defense responses in M...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28607058/host-secreted-antimicrobial-peptide-enforces-symbiotic-selectivity-in-medicago-truncatula
#7
Qi Wang, Shengming Yang, Jinge Liu, Kata Terecskei, Edit Ábrahám, Anikó Gombár, Ágota Domonkos, Attila Szűcs, Péter Körmöczi, Ting Wang, Lili Fodor, Linyong Mao, Zhangjun Fei, Éva Kondorosi, Péter Kaló, Attila Kereszt, Hongyan Zhu
Legumes engage in root nodule symbioses with nitrogen-fixing soil bacteria known as rhizobia. In nodule cells, bacteria are enclosed in membrane-bound vesicles called symbiosomes and differentiate into bacteroids that are capable of converting atmospheric nitrogen into ammonia. Bacteroid differentiation and prolonged intracellular survival are essential for development of functional nodules. However, in the Medicago truncatula-Sinorhizobium meliloti symbiosis, incompatibility between symbiotic partners frequently occurs, leading to the formation of infected nodules defective in nitrogen fixation (Fix(-))...
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28607056/microsymbiont-discrimination-mediated-by-a-host-secreted-peptide-in-medicago-truncatula
#8
Shengming Yang, Qi Wang, Elena Fedorova, Jinge Liu, Qiulin Qin, Qiaolin Zheng, Paul A Price, Huairong Pan, Dong Wang, Joel S Griffitts, Ton Bisseling, Hongyan Zhu
The legume-rhizobial symbiosis results in the formation of root nodules that provide an ecological niche for nitrogen-fixing bacteria. However, plant-bacteria genotypic interactions can lead to wide variation in nitrogen fixation efficiency, and it is not uncommon that a bacterial strain forms functional (Fix(+)) nodules on one plant genotype but nonfunctional (Fix(-)) nodules on another. Host genetic control of this specificity is unknown. We herein report the cloning of the Medicago truncatula NFS1 gene that regulates the fixation-level incompatibility with the microsymbiont Sinorhizobium meliloti Rm41...
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28559336/arac-like-transcriptional-activator-cuxr-binds-c-di-gmp-by-a-pilz-like-mechanism-to-regulate-extracellular-polysaccharide-production
#9
Simon Schäper, Wieland Steinchen, Elizaveta Krol, Florian Altegoer, Dorota Skotnicka, Lotte Søgaard-Andersen, Gert Bange, Anke Becker
Cyclic dimeric GMP (c-di-GMP) has emerged as a key regulatory player in the transition between planktonic and sedentary biofilm-associated bacterial lifestyles. It controls a multitude of processes including production of extracellular polysaccharides (EPSs). The PilZ domain, consisting of an N-terminal "RxxxR" motif and a β-barrel domain, represents a prototype c-di-GMP receptor. We identified a class of c-di-GMP-responsive proteins, represented by the AraC-like transcription factor CuxR in plant symbiotic α-proteobacteria...
June 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28547343/msmir156-affects-global-gene-expression-and-promotes-root-regenerative-capacity-and-nitrogen-fixation-activity-in-alfalfa
#10
Banyar Aung, Ruimin Gao, Margaret Y Gruber, Ze-Chun Yuan, Mark Sumarah, Abdelali Hannoufa
MicroRNA156 (miR156) regulates a network of downstream genes to affect plant growth and development. We previously generated alfalfa (Medicago sativa) plants that overexpress homologous miR156 (MsmiR156OE), and identified three of its SPL target genes. These plants exhibited increased vegetative yield, delayed flowering and longer roots. In this study, we aimed to elucidate the effect of miR156 on the root system, including effect on nodulation and nitrogen fixation. We found that MsmiR156 overexpression increases root regeneration capacity in alfalfa, but with little effect on root biomass at the early stages of root development...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28533217/transcriptional-control-of-the-lateral-flagellar-genes-of-bradyrhizobium-diazoefficiens
#11
Elías J Mongiardini, J Ignacio Quelas, Carolina Dardis, M Julia Althabegoiti, Aníbal R Lodeiro
Bradyrhizobium diazoefficiens, the soybean N2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. Here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. We found that those genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. Among the monocistronic units is blr6846, homologous to the Class-IB master regulators of flagella synthesis in Brucella melitensis and Ensifer meliloti, and required for the expression of all the lateral-flagellar genes except lafA2, which locus encodes a single lateral flagellin...
May 22, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28489251/geographically-structured-genetic-variation-in-the-medicago-lupulina-ensifer-mutualism
#12
Tia L Harrison, Corlett W Wood, Katy D Heath, John R Stinchcombe
Gene flow between genetically differentiated populations can maintain variation in species interactions, especially when population structure is congruent between interacting species. However, large-scale empirical comparisons of the population structure of interacting species are rare, particularly in positive interspecific interactions (mutualisms). One agriculturally and ecologically important mutualism is the partnership between legume plants and rhizobia. Through characterizing and comparing the population genomic structure of the legume Medicago lupulina and two rhizobial species (Ensifer medicae and E...
May 10, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28461447/bacterial-pero-permeases-transport-sulfate-and-related-oxyanions
#13
Marie-Christine Hoffmann, Yvonne Pfänder, Marc Tintel, Bernd Masepohl
Rhodobacter capsulatus synthesizes the high-affinity ABC transporters CysTWA and ModABC to specifically import the chemically related oxyanions sulfate and molybdate, respectively. In addition, R. capsulatus has the low-affinity permease PerO acting as a general oxyanion transporter, whose elimination increases tolerance to molybdate and tungstate. Although PerO-like permeases are widespread in bacteria, their function has not been examined in any other species to date. Here, we present evidence that PerO permeases from the alphaproteobacteria Agrobacterium tumefaciens, Dinoroseobacter shibae, Rhodobacter sphaeroides, and Sinorhizobium meliloti and the gammaproteobacterium Pseudomonas stutzeri functionally substitute for R...
July 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28437159/specific-host-responsive-associations-between-medicago-truncatula-accessions-and-sinorhizobium-strains
#14
Théophile Kazmierczak, Marianna Nagymihály, Florian Lamouche, Quentin Barrière, Ibtissem Guefrachi, Benoit Alunni, Mouna Ouadghiri, Jamal Ibijbijen, Éva Kondorosi, Peter Mergaert, Véronique Gruber
Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impact on the efficiency of nodule functioning and nitrogen-fixation activity. The model legume Medicago truncatula forms nodules with several species of the Sinorhizobium genus. However, the efficiency of these bacterial strains is highly variable...
May 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28417169/rational-design-of-substrate-binding-pockets-in-polyphosphate-kinase-for-use-in-cost-effective-atp-dependent-cascade-reactions
#15
Hao Cao, Kaili Nie, Chengcheng Li, Haijun Xu, Fang Wang, Tianwei Tan, Luo Liu
Adenosine-5'-triphosphate (ATP) is the energy equivalent of the living system. Polyphosphate (polyP) is the ancient energy storage equivalent of organisms. Polyphosphate kinases (PPKs) catalyze the polyP formation or ATP formation, to store energy or to regenerate ATP, respectively. However, most PPKs are active only in the presence of long polyPs, which are more difficult and more expensive to generate than the short polyPs. We investigated the PPK preference towards polyPs by site-directed mutagenesis and computational simulation, to understand the mechanism and further design enzymes for effective ATP regeneration using short polyPs for in vitro cascade reactions, which are highly desired for research and applications...
July 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28416708/phou-allows-rapid-adaptation-to-high-phosphate-concentrations-by-modulating-pstscab-transport-rate-in-sinorhizobium-meliloti
#16
George C diCenzo, Harsh Sharthiya, Anish Nanda, Maryam Zamani, Turlough M Finan
Maintenance of cellular phosphate homeostasis is essential for cellular life. The PhoU protein has emerged as a key regulator of this process in bacteria and it is suggested to modulate phosphate import by PstSCAB and control activation of the phosphate limitation response by the PhoR-PhoB two-component system. However, a proper understanding of PhoU has remained elusive due to numerous complications of mutating phoU, including loss of viability and genetic instability of the mutants. Here, we developed two sets of strains of Sinorhizobium meliloti that overcame these limitations and allowed for a more detailed and comprehensive analysis of the biological and molecular activities of PhoU...
April 17, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28401641/a-conserved-%C3%AE-proteobacterial-small-rna-contributes-to-osmoadaptation-and-symbiotic-efficiency-of-rhizobia-on-legume-roots
#17
Marta Robledo, Alexandra Peregrina, Vicenta Millán, Natalia I García-Tomsig, Omar Torres-Quesada, Pedro F Mateos, Anke Becker, José I Jiménez-Zurdo
Small non-coding RNAs (sRNAs) are expected to have pivotal roles in the adaptive responses underlying symbiosis of nitrogen-fixing rhizobia with legumes. Here, we provide primary insights into the function and activity mechanism of the Sinorhizobium meliloti trans-sRNA NfeR1 (Nodule Formation Efficiency RNA). Northern blot probing and transcription tracking with fluorescent promoter-reporter fusions unveiled high nfeR1 expression in response to salt stress and throughout the symbiotic interaction. The strength and differential regulation of nfeR1 transcription are conferred by a motif, which is conserved in nfeR1 promoter regions in α-proteobacteria...
April 12, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28398840/the-ntry-ntrx-system-of-sinorhizobium-meliloti-gr4-regulates-motility-eps-i-production-and-nitrogen-metabolism-but-is-dispensable-for-symbiotic-nitrogen-fixation
#18
Nieves Calatrava-Morales, Joaquina Nogales, Kinia Ameztoy, Bart van Steenbergen, María J Soto
Sinorhizobium meliloti can translocate over surfaces. However, little is known about the regulatory mechanisms that control this trait and its relevance for establishing symbiosis with alfalfa plants. To gain insights into this field, we isolated Tn5 mutants of S. meliloti GR4 with impaired surface motility. In mutant strain GRS577, the transposon interrupted the ntrY gene encoding the sensor kinase of the NtrY/NtrX two-component regulatory system. GRS577 is impaired in flagella synthesis and overproduces succinoglycan, which is responsible for increased biofilm formation...
July 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28398123/heterologous-complementation-reveals-a-specialized-activity-for-baca-in-the-medicago-sinorhizobium-meliloti-symbiosis
#19
George C diCenzo, Maryam Zamani, Hannah N Ludwig, Turlough M Finan
The bacterium Sinorhizobium meliloti Rm2011 forms N2-fixing root nodules on alfalfa and other leguminous plants. The pSymB chromid contains a 110-kb region (the ETR region) showing high synteny to a chromosomally located region in Sinorhizobium fredii NGR234 and related rhizobia. We recently introduced the ETR region from S. fredii NGR234 into the S. meliloti chromosome. Here, we report that, unexpectedly, the S. fredii NGR234 ETR region did not complement deletion of the S. meliloti ETR region in symbiosis with Medicago sativa...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28394919/correction-quantitative-proteomic-analysis-of-the-hfq-regulon-in-sinorhizobium-meliloti-2011
#20
Patricio Sobrero, Jan-Philip Schlüter, Ulrike Lanner, Andreas Schlosser, Anke Becker, Claudio Valverde
[This corrects the article DOI: 10.1371/journal.pone.0048494.].
2017: PloS One
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