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https://www.readbyqxmd.com/read/29783703/regulation-mediated-by-n-acyl-homoserine-lactone-quorum-sensing-signals-in-the-rhizobium-legume-symbiosis
#1
REVIEW
Nieves Calatrava-Morales, Matthew McIntosh, María J Soto
Soil-dwelling bacteria collectively referred to as rhizobia synthesize and perceive N -acyl-homoserine lactone (AHL) signals to regulate gene expression in a population density-dependent manner. AHL-mediated signaling in these bacteria regulates several functions which are important for the establishment of nitrogen-fixing symbiosis with legume plants. Moreover, rhizobial AHL act as interkingdom signals triggering plant responses that impact the plant-bacteria interaction. Both the regulatory mechanisms that control AHL synthesis in rhizobia and the set of bacterial genes and associated traits under quorum sensing (QS) control vary greatly among the rhizobial species...
May 18, 2018: Genes
https://www.readbyqxmd.com/read/29783033/coordination-of-symbiosis-and-cell-cycle-functions-in-sinorhizobium-meliloti
#2
REVIEW
Shuanghong Xue, Emanuele G Biondi
The symbiotic nitrogen fixing species Sinorhizobium meliloti represents a remarkable model system for the class Alphaproteobacteria, which includes genera such as Caulobacter, Agrobacterium and Brucella. It is capable of living free in the soil, and is also able to establish a complex symbiosis with leguminous plants, during which its cell cycle program is completely rewired presumably due, at least in part, to the action of peptides secreted by the plant. Here we will discuss how the cell cycle regulation works in S...
May 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29777364/cell-division-and-turgor-mediate-enhanced-plant-growth-in-arabidopsis-plants-treated-with-the-bacterial-signalling-molecule-lumichrome
#3
Motlalepula Pholo, Beatrix Coetzee, Hans J Maree, Philip R Young, James R Lloyd, Jens Kossmann, Paul N Hills
Transcriptomic analysis indicates that the bacterial signalling molecule lumichrome enhances plant growth through a combination of enhanced cell division and cell enlargement, and possibly enhances photosynthesis. Lumichrome (7,8 dimethylalloxazine), a novel multitrophic signal molecule produced by Sinorhizobium meliloti bacteria, has previously been shown to elicit growth promotion in different plant species (Phillips et al. in Proc Natl Acad Sci USA 96:12275-12280, https://doi.org/10.1073/pnas.96.22.12275 , 1999)...
May 17, 2018: Planta
https://www.readbyqxmd.com/read/29751749/engineering-a-vitamin-b-12-high-throughput-screening-system-by-riboswitch-sensor-in-sinorhizobium-meliloti
#4
Yingying Cai, Miaomiao Xia, Huina Dong, Yuan Qian, Tongcun Zhang, Beiwei Zhu, Jinchuan Wu, Dawei Zhang
BACKGROUND: As a very important coenzyme in the cell metabolism, Vitamin B12 (cobalamin, VB12 ) has been widely used in food and medicine fields. The complete biosynthesis of VB12 requires approximately 30 genes, but overexpression of these genes did not result in expected increase of VB12 production. High-yield VB12 -producing strains are usually obtained by mutagenesis treatments, thus developing an efficient screening approach is urgently needed. RESULT: By the help of engineered strains with varied capacities of VB12 production, a riboswitch library was constructed and screened, and the btuB element from Salmonella typhimurium was identified as the best regulatory device...
May 11, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29743820/use-of-rna-immunoprecipitation-method-for-determining-sinorhizobium-meliloti-rna-hfq-protein-associations-in-vivo
#5
Mengsheng Gao, Anne Benge, Julia M Mesa, Regina Javier, Feng-Xia Liu
Background: Soil bacterium Sinorhizobium meliloti ( S. meliloti ) forms an endosymbiotic partnership with Medicago truncatula ( M. truncatula ) roots which results in root nodules. The bacteria live within root nodules where they function to fix atmospheric N2 and supply the host plant with reduced nitrogen. The bacterial RNA-binding protein Hfq (Hfq) is an important regulator for the effectiveness of the nitrogen fixation. RNA immunoprecipitation (RIP) method is a powerful method for detecting the association of Hfq protein with specific RNA in cultured bacteria, yet a RIP method for bacteria living in root nodules remains to be described...
2018: Biological Procedures Online
https://www.readbyqxmd.com/read/29737549/effect-of-pb-resistant-plant-growth-promoting-rhizobacteria-inoculation-on-growth-and-lead-uptake-by-lathyrus-sativus
#6
Souhir Abdelkrim, Salwa H Jebara, Omar Saadani, Manel Chiboub, Ghassen Abid, Moez Jebara
In search of efficient and resistant plant growth-promoting rhizobacteria (PGPR) strains with multiple activities, a total of twelve bacterial belonging to R. leguminosarum, S. meliloti, Pseudomonas sp., P. fluorescens, Luteibacter sp., Variovorax sp., B. simplex, and B. megaterium were isolated from root nodules of grass pea (Lathyrus sativus L.) grown in contaminated soils. Upon screening, all test strains were able to synthesize indoleacetic acid; more than 90% were siderophore producers and 75% showed varying levels of phosphate solubilizing ability...
May 8, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29724854/complete-genome-sequence-of-sinorhizobium-meliloti-bacteriophage-hmsp1-susan
#7
Brennon Fleagle, Aldina Imamovic, Silvia Toledo, Madison Couves, Autumn Jensen, Mandy Vang, Ashley Steevens, Nevin D Young, Michael J Sadowsky, Betsy M Martinez-Vaz
Sinorhiozbium bacteriophage HMSP1-Susan has a genome of 51,963 bp in size, with a GC content of 52.5%. It contains 97 putative coding sequences; 83% of these coding sequences (CDS) encode proteins classified as hypothetical or having unknown functions. HMSP1 has limited homology to previously reported viruses and likely represents a new phage that infects this nitrogen-fixing bacterium.
May 3, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29684389/high-quality-forage-production-under-salinity-by-using-a-salt-tolerant-atnxh1-expressing-transgenic-alfalfa-combined-with-a-natural-stress-resistant-nitrogen-fixing-bacterium
#8
Margarita Stritzler, Pagano Elba, Carolina Berini, Cristina Gomez, Nicolás Ayub, Gabriela Soto
Alfalfa, usually known as the "Queen of Forages", is the main source of vegetable protein to meat and milk production systems worldwide. This legume is extremely rich in proteins due to its highly efficient symbiotic association with nitrogen-fixing strains. In the last years, alfalfa culture has been displaced to saline environments by other important crops, including major cereals, a fact that has reduced its biomass production and symbiotic nitrogen fixation. In this short communication, we report the high forage production and nutrient quality of alfalfa under saline conditions by alfalfa transformation with the AtNHX1 Na+ /H+ antiporter and inoculation with the stress-resistant nitrogen-fixing strain Sinorhizobium meliloti B401...
April 20, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29672509/robustness-encoded-across-essential-and-accessory-replicons-of-the-ecologically-versatile-bacterium-sinorhizobium-meliloti
#9
George C diCenzo, Alex B Benedict, Marco Fondi, Graham C Walker, Turlough M Finan, Alessio Mengoni, Joel S Griffitts
Bacterial genome evolution is characterized by gains, losses, and rearrangements of functional genetic segments. The extent to which large-scale genomic alterations influence genotype-phenotype relationships has not been investigated in a high-throughput manner. In the symbiotic soil bacterium Sinorhizobium meliloti, the genome is composed of a chromosome and two large extrachromosomal replicons (pSymA and pSymB, which together constitute 45% of the genome). Massively parallel transposon insertion sequencing (Tn-seq) was employed to evaluate the contributions of chromosomal genes to growth fitness in both the presence and absence of these extrachromosomal replicons...
April 19, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29671722/the-complete-replicons-of-16-ensifer-meliloti-strains-offer-insights-into-intra-and-inter-replicon-gene-transfer-transposon-associated-loci-and-repeat-elements
#10
Matthew Nelson, Joseph Guhlin, Brendan Epstein, Peter Tiffin, Michael J Sadowsky
Ensifer meliloti (formerly Rhizobium meliloti and Sinorhizobium meliloti) is a model bacterium for understanding legume-rhizobial symbioses. The tripartite genome of E. meliloti consists of a chromosome, pSymA and pSymB, and in some instances strain-specific accessory plasmids. The majority of previous sequencing studies have relied on the use of assemblies generated from short read sequencing, which leads to gaps and assembly errors. Here we used PacBio-based, long-read assemblies and were able to assemble, de novo, complete circular replicons...
April 19, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29670598/contribution-of-mobile-group-ii-introns-to-sinorhizobium-meliloti-genome-evolution
#11
REVIEW
Nicolás Toro, Francisco Martínez-Abarca, María D Molina-Sánchez, Fernando M García-Rodríguez, Rafael Nisa-Martínez
Mobile group II introns are ribozymes and retroelements that probably originate from bacteria. Sinorhizobium meliloti , the nitrogen-fixing endosymbiont of legumes of genus Medicago , harbors a large number of these retroelements. One of these elements, RmInt1, has been particularly successful at colonizing this multipartite genome. Many studies have improved our understanding of RmInt1 and phylogenetically related group II introns, their mobility mechanisms, spread and dynamics within S. meliloti and closely related species...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29619919/polyamines-are-required-for-normal-growth-in-sinorhizobium-meliloti
#12
Victor A Becerra-Rivera, Ed Bergström, Jane Thomas-Oates, Michael F Dunn
Polyamines (PAs) are ubiquitous polycations derived from basic l-amino acids whose physiological roles are still being defined. Their biosynthesis and functions in nitrogen-fixing rhizobia such as Sinorhizobium meliloti have not been extensively investigated. Thin layer chromatographic and mass spectrometric analyses showed that S. meliloti Rm8530 produces the PAs, putrescine (Put), spermidine (Spd) and homospermidine (HSpd), in their free forms and norspermidine (NSpd) in a form bound to macromolecules. The S...
April 2018: Microbiology
https://www.readbyqxmd.com/read/29582151/bacterial-exopolysaccharides-as-a-modern-biotechnological-tool-for-modification-of-fungal-laccase-properties-and-metal-ion-binding
#13
Monika Osińska-Jaroszuk, Magdalena Jaszek, Magdalena Starosielec, Justyna Sulej, Anna Matuszewska, Monika Janczarek, Renata Bancerz, Jerzy Wydrych, Adrian Wiater, Anna Jarosz-Wilkołazka
Four bacterial EPSs extracted from Rhizobium leguminosarum bv. trifolii Rt24.2, Sinorhizobium meliloti Rm1021, Bradyrhizobium japonicum USDA110, and Bradyrhizobium elkanii USDA76 were determined towards their metal ion adsorption properties and possible modification of Cerrena unicolor laccase properties. The highest magnesium and iron ion-sorption capacity (~ 42 and ~ 14.5%, respectively) was observed for EPS isolated from B. japonicum USDA110. An evident influence of EPSs on the stability of laccase compared to the control values (without EPSs) was shown after 30-day incubation at 25 °C...
March 26, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29563186/inter-replicon-gene-flow-contributes-to-transcriptional-integration-in-the-sinorhizobium-meliloti-multipartite-genome
#14
George C diCenzo, Deelaka Wellappili, G Brian Golding, Turlough M Finan
Integration of newly acquired genes into existing regulatory networks is necessary for successful horizontal gene transfer (HGT). Ten percent of bacterial species contain at least two DNA replicons over 300 kilobases in size, with the secondary replicons derived predominately through HGT. The Sinorhizobium meliloti genome is split between a 3.7 Mb chromosome, a 1.7 Mb chromid consisting largely of genes acquired through ancient HGT, and a 1.4 Mb megaplasmid consisting primarily of recently acquired genes. Here, RNA-sequencing is used to examine the transcriptional consequences of massive, synthetic genome reduction produced through the removal of the megaplasmid and/or the chromid...
May 4, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29547354/the-lsrb-protein-is-required-for-agrobacterium-tumefaciens-interaction-with-host-plants
#15
Guirong Tang, Qiong Li, Shenghui Xing, Ningning Li, Zheng Tang, Liangliang Yu, Junhui Yan, Xuan Li, Li Luo
Agrobacterium tumefaciens infects and causes crown galls in dicot plants by transferring T-DNA from the Ti plasmid to the host plant via a type IV secretion system (T4SS). This process requires appropriate environmental conditions, certain plant secretions and bacterial regulators. In our previous work, a member of the LysR family of transcriptional regulators (LsrB) in Sinorhizobium meliloti was found to modulate its symbiotic interactions with the host plant alfalfa. However, the function of its homologue in A...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29531182/nsra-a-predicted-%C3%AE-barrel-outer-membrane-protein-involved-in-plant-signal-perception-and-the-control-of-secondary-infection-in-sinorhizobium-meliloti
#16
Anne-Marie Garnerone, Fernando Sorroche, Lan Zou, Céline Mathieu-Demazière, Chang Fu Tian, Catherine Masson-Boivin, Jacques Batut
An ongoing signal exchange fine-tunes the symbiotic interaction between rhizobia and legumes, ensuring the establishment and maintenance of mutualism. In a recently identified regulatory loop, endosymbiotic S. meliloti exert a negative feedback on root infection in response to unknown plant cues. Upon signal perception, three bacterial Adenylate Cyclases (ACs) of the inner membrane, CyaD1, CyaD2 and CyaK, synthesize the second messenger cAMP that, together with the cAMP-dependent Clr transcriptional activator, activates the expression of genes involved in root infection control...
March 12, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29488306/2-tridecanone-impacts-surface-associated-bacterial-behaviours-and-hinders-plant-bacteria-interactions
#17
Isabel M López-Lara, Joaquina Nogales, Ángel Pech-Canul, Nieves Calatrava-Morales, Lydia M Bernabéu-Roda, Paloma Durán, Virginia Cuéllar, José Olivares, Laura Alvarez, Diana Palenzuela-Bretones, Manuel Romero, Stephan Heeb, Miguel Cámara, Otto Geiger, María J Soto
Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha-proteobacterium Sinorhizobium meliloti, the lack of long-chain fatty acyl-coenzyme A synthetase activity (FadD) leads to increased surface motility, defects in biofilm development and impaired root colonization. In this study, analyses of lipid extracts and volatiles revealed that a fadD mutant accumulates 2-tridecanone (2-TDC), a methylketone (MK) known as a natural insecticide...
February 28, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29484603/identification-of-small-rna-protein-partners-in-plant-symbiotic-bacteria
#18
Marta Robledo, Ana M Matia-González, Natalia I García-Tomsig, José I Jiménez-Zurdo
The identification of the protein partners of bacterial small noncoding RNAs (sRNAs) is essential to understand the mechanistic principles and functions of riboregulation in prokaryotic cells. Here, we describe an optimized affinity chromatography protocol that enables purification of in vivo formed sRNA-protein complexes in Sinorhizobium meliloti, a genetically tractable nitrogen-fixing plant symbiotic bacterium. The procedure requires the tagging of the desired sRNA with the MS2 aptamer, which is affinity-captured by the MS2-MBP protein conjugated to an amylose resin...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29467773/characterization-of-novel-plant-symbiosis-mutants-using-a-new-multiple-gene-expression-reporter-sinorhizobium-meliloti-strain
#19
Claus Lang, Lucinda S Smith, Sharon R Long
The formation of nitrogen fixing root nodules by Medicago truncatula and Sinorhizobium meliloti requires communication between both organisms and coordinated differentiation of plant and bacterial cells. After an initial signal exchange, the bacteria invade the tissue of the growing nodule via plant-derived tubular structures, called infection threads. The bacteria are released from the infection threads into invasion-competent plant cells, where they differentiate into nitrogen-fixing bacteroids. Both organisms undergo dramatic transcriptional, metabolic and morphological changes during nodule development...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29462313/overexpression-of-the-periplasmic-nitrate-reductase-supports-anaerobic-growth-by-ensifer-meliloti
#20
María J Torres, Sergio Avila, Eulogio J Bedmar, María J Delgado
The alfalfa endosymbiont Ensifer meliloti strain1021 is known to be an incomplete denitrifier due to its inability to grow anoxically using nitrate as respiratory substrate to produce ATP and grow under anoxic conditions. Although this bacterium contains and expresses the complete set of denitrification genes napEFDABC, nirK, norECBQD and nosRZDFYLX encoding the periplasmic nitrate reductase (Nap), Cu-containing nitrite reductase (NirK), c-type nitric oxide (cNor) and nitrous oxide reductase (Nos), respectively, the reasons of its inability to grow under anoxic conditions are still very poorly understood...
April 1, 2018: FEMS Microbiology Letters
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