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https://www.readbyqxmd.com/read/27911225/successful-treatment-of-mixed-yolk-sac-tumor-and-mature-teratoma-in-the-spinal-cord-case-report
#1
Akitake Mukasa, Shunsuke Yanagisawa, Kuniaki Saito, Shota Tanaka, Keisuke Takai, Junji Shibahara, Masachika Ikegami, Yusuke Nakao, Katsushi Takeshita, Masao Matsutani, Nobuhito Saito
Primary spinal germ cell tumors are rare, and spinal nongerminomatous germ cell tumors represent an even rarer subset for which no standard therapy has been established. The authors report the case of a 24-year-old woman with multifocal primary spinal germ cell tumors scattered from T-12 to L-5 that consisted of yolk sac tumor and mature teratoma. After diagnostic partial resection, the patient was treated with 30 Gy of craniospinal irradiation and 30 Gy of local spinal irradiation, followed by 8 courses of chemotherapy based on ifosfamide, cisplatin, and etoposide (ICE)...
December 2, 2016: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/27902895/pathogeny-of-fatigued-walking-condition-in-pekin-ducks
#2
M J Da Costa, E O Oviedo-Rondón, M Wineland, D Jeffrey
Fatigued walking condition (FWC) in ducks is an important welfare and processing issue during the loading/unloading to the processing plant that can be related to heart and bone development. An experiment was conducted to evaluate the effects of incubation conditions on duck embryo bone and heart development and their subsequent effects on ducks showing FWC at market age. Four groups of 2500 Pekin duck eggs were subjected to combinations of two incubation temperature profiles (elevated [E] and normal [N]) and two eggshell conductance profiles (G) (reduced [GR] and normal [GN])...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27876644/dinotefuran-induced-morphophysiological-changes-in-semi-engorged-females-rhipicephalus-sanguineus-latreille-1806-acari-ixodidae-ticks-ultra-structural-evaluation
#3
Patrícia Rosa de Oliveira, Luis Adriano Anholeto, Gerváso Henrique Bechara, Maria Izabel Camargo Mathias
The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet(®)) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days...
November 19, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27871459/morphology-histochemistry-and-glycosylation-of-the-placenta-and-associated-tissues-in-the-european-hedgehog-erinaceus-europaeus
#4
Carolyn J P Jones, A M Carter, W R Allen, Sandra A Wilsher
INTRODUCTION: There are few descriptions of the placenta and associated tissues of the European hedgehog (Erinaceus europaeus) and here we present findings on a near-term pregnant specimen. METHODS: Tissues were examined grossly and then formalin fixed and wax-embedded for histology and immunocytochemistry (cytokeratin) and resin embedded for lectin histochemistry. RESULTS: Each of four well-developed and near term hoglets displayed a discoid, haemochorial placenta with typical labyrinth and spongy zones...
December 2016: Placenta
https://www.readbyqxmd.com/read/27861143/intratumoral-heterogeneity-and-chemoresistance-in-nonseminomatous-germ-cell-tumor-of-the-testis
#5
Mehmet Asim Bilen, Kenneth R Hess, Matthew T Campbell, Jennifer Wang, Russell R Broaddus, Jose A Karam, John F Ward, Christopher G Wood, Seungtaek L Choi, Priya Rao, Miao Zhang, Aung Naing, Rosale General, Diana H Cauley, Sue-Hwa Lin, Christopher J Logothetis, Louis L Pisters, Shi-Ming Tu
BACKGROUND: Nonseminomatous germ cell tumor of the testis (NSGCT) is largely curable. However, a small group of patients develop refractory disease. We investigated the hypothesis that intratumoral heterogeneity contributes to the emergence of chemoresistance and the development of refractory tumor subtypes. RESULTS: Our institution's records for January 2000 through December 2010 included 275 patients whose primary tumor showed pure embryonal carcinoma (pure E); mixed embryonal carcinoma, yolk sac tumor, and teratoma (EYT); or mixed embryonal carcinoma, yolk sac tumor, seminoma, and teratoma (EYST)...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27859263/post-chemotherapy-retroperitoneal-teratoma-in-nonseminomatous-germ-cell-tumors-do-predictive-factors-exist-results-from-a-national-multicenter-study
#6
Marie Dusaud, Bernard Malavaud, Younes Bayoud, Philippe Sebe, Jean Luc Hoepffner, Laurent Salomon, Alain Houlgatte, Géraldine Pignot, Jérome Rigaud, Aude Fléchon, Christian Pfister, Morgan Rouprêt, Michel Soulié, Arnaud Méjean, Xavier Durand
BACKGROUND AND OBJECTIVES: To identify predictive preoperative factors of the presence of teratoma in retroperitoneal lymph node dissection specimens. METHODS: We performed a 20 years multicenter retrospective analysis of all patients who underwent retroperitoneal lymph node dissection for residual masses after chemotherapy (PC-RPLND). Patients had undergone PC-RPLND after chemotherapy for advanced testicular cancer. The histologic components of the primary tumor were compared with those of the residual masses using logistic regression...
November 18, 2016: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27807495/giant-mediastinal-germ-cell-tumour-an-enigma-of-surgical-consideration
#7
Firdaus Hayati, Nurayub Mohd Ali, Levin Kesu Belani, Nornazirah Azizan, Andee Dzulkarnaen Zakaria, Mohd Ramzisham Abdul Rahman
We present a case of 16-year-old male, who was referred from private centre for dyspnoea, fatigue, and orthopnea. The chest radiograph revealed complete opacification of left chest which was confirmed by computed tomography as a large left mediastinal mass measuring 14 × 15 × 18 cm. The diagnostic needle core biopsy revealed mixed germ cell tumour with possible combination of embryonal carcinoma, yolk sac, and teratoma. After 4 cycles of neoadjuvant BEP regime, there was initial response of tumour markers but not tumour bulk...
2016: Case Reports in Surgery
https://www.readbyqxmd.com/read/27802161/elys-regulates-the-localization-of-lbr-by-modulating-its-phosphorylation-state
#8
Yasuhiro Mimura, Masatoshi Takagi, Michaela Clever, Naoko Imamoto
Lamin B receptor (LBR), an inner nuclear membrane (INM) protein, contributes to the functional integrity of the nucleus by tethering heterochromatin to the nuclear envelope. We have previously reported that the depletion of embryonic large molecule derived from yolk sac (ELYS; also known as AHCTF1), a component of the nuclear pore complex, from cells perturbs the localization of LBR to the INM, but little is known about the underlying molecular mechanism. In this study, we found that the depletion of ELYS promoted LBR phosphorylation at the residues known to be phosphorylated by cyclin-dependent kinase (CDK) and serine/arginine protein kinases 1 and 2 (SRPK1 and SRPK2, respectively)...
November 15, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27798931/radiation-pneumonitis-changes-over-time-after-stereotactic-body-radiation-therapy-for-lung-tumors-post-treatment-cavity-sunny-side-up-egg-like-changes
#9
Toshiya Maebayashi, Naoya Ishibashi, Takuya Aizawa, Masakuni Sakaguchi, Tsutomu Sato, Jiro Kawamori, Yoshiaki Tanaka
BACKGROUND: Stereotactic body radiation therapy (SBRT) is a viable treatment option for stage I non-small cell lung carcinoma (NSCLC) in non-surgical candidates. Although treatment with a 100 Gy biological effective dose is considered to carry a low risk of local recurrence, only a few reports have focused on changes in tumors or surrounding intact portions of the lung after SBRT. Such changes are assessed using diagnostic imaging specifically for radiation pneumonitis, which can be difficult to distinguish from local tumor recurrence...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27778488/robot-assisted-laparoscopic-retroperitoneal-lymph-node-dissection-in-testicular-tumor
#10
Fabio C M Torricelli, Denis Jardim, Giuliano B Guglielmetti, Vipul Patel, Rafael F Coelho
INTRODUCTION AND OBJECTIVE: Retroperitoneal lymph node dissection (RPLND) is indicated for patients with non-seminoma¬tous germ cell tumor (NSGCT) with residual disease after chemotherapy. Although the gold standard approach is still the open surgery, few cases of robot-assisted laparoscopic RPLND have been described. Herein, we aim to present the surgical technique for robot-assisted laparoscopic RPLND. PATIENT AND METHOD: A 30 year-old asymptomatic man presented with left testicular swelling for 2 months...
October 25, 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/27702419/expression-of-the-t85a-mutant-of-zebrafish-aquaporin-3b-improves-post-thaw-survival-of-cryopreserved-early-mammalian-embryos
#11
Sylvia J Bedford-Guaus, François Chauvigné, Eva Mejía-Ramírez, Mercè Martí, Antoni Ventura-Rubio, Ángel Raya, Joan Cerdà, Anna Veiga
While vitrification has become the method of choice for preservation of human oocytes and embryos, cryopreservation of complex tissues and of large yolk-containing cells, remains largely unsuccessful. One critical step in such instances is appropriate permeation while avoiding potentially toxic concentrations of cryoprotectants. Permeation of water and small non-charged solutes, such as those used as cryoprotectants, occurs largely through membrane channel proteins termed aquaporins (AQPs). Substitution of a Thr by an Ala residue in the pore-forming motif of the zebrafish (Dario rerio) Aqp3b paralog resulted in a mutant (DrAqp3b-T85A) that when expressed in Xenopus or porcine oocytes increased their permeability to ethylene glycol at pH 7...
October 5, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27439963/effects-of-in-ovo-feeding-with-glycerol-for-broilers
#12
D G Neves, P L Retes, R R Rocha, L G Ferreira, L P Naves, R R Alvarenga, E J Fassani, L J Pereira, R V Sousa, M G Zangeronimo
The objective was to evaluate the effect of in ovo feeding with glycerol on post-hatch development in broiler chicks. A total of 408 fertile eggs were divided into six experimental groups consisting of five 0.9% saline solutions containing various concentrations of glycerol (12.5, 25.0, 37.5 and 50.0 nmol/ml), and a placebo group (inoculation with saline only) and a control group (without inoculation). Inoculations were performed at 17 days of incubation for the evaluation of hatchability, embryo mortality, body and viscera weights, intestinal epithelium morphometry, blood glucose and liver glycerol kinase activity of chicks at hatching...
July 21, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27409335/sida-tuberculata-malvaceae-a-study-based-on-development-of-extractive-system-and-in-silico-and-in-vitro-properties
#13
H S da Rosa, A C F Salgueiro, A Z C Colpo, F R Paula, A S L Mendez, V Folmer
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions...
July 11, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27385583/controlled-synthesis-of-au-agau-yolk-shell-cuboctahedra-with-well-defined-facets
#14
Alejandra Londono-Calderon, Daniel Bahena, Miguel J Yacaman
The synthesis of Au@AgAu yolk-shell cuboctahedra nanoparticles formed by galvanic replacement in a seed-mediated method is described. Initially, single-crystal Au seeds are used for the formation of Au@Ag core-shell nanocubes, which serve as the template material for the deposition of an external Au layer. The well-controlled synthesis yields the formation of cuboctahedra nanoparticles with smooth inner and outer Au/Ag surfaces. The deposition/oxidation process is described to understand the formation of cuboctahedra and octahedra nanoparticles...
August 2, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27352567/ovarian-yolk-sac-tumor-in-postmenopausal-females-a-report-of-five-cases-and-a-literature-review
#15
REVIEW
X Wang, J He, Y Li
PURPOSE OF INVESTIGATION: Little is known regarding ovarian yolk sac tumor (YST) in postmenopausal females. The authors aimed to investigate the clinical features and prognosis of postmenopausal patients with ovarian YST. MATERIALS AND METHODS: Five cases of ovarian YST in postmenopausal females were retrospectively reviewed. A MEDLINE search was performed to identify postmenopausal patients with ovarian YST. RESULTS: Four patients had Stage I or II disease (Stage IC, IIC, and IIB in one, two, and one patient, respectively), and one patient demonstrated an unclear stage...
2016: European Journal of Gynaecological Oncology
https://www.readbyqxmd.com/read/27261260/molecular-characterization-and-developmental-expression-patterns-of-apolipoprotein-a-i-in-senegalese-sole-solea-senegalensis-kaup
#16
J Román-Padilla, A Rodríguez-Rúa, M Manchado, I Hachero-Cruzado
The apolipoprotein A-I (ApoA-I) is an essential component of the high density lipoproteins (HDL). In this study, the cDNA and genomic sequences of this apolipoprotein were characterized for first time in Solea senegalensis. The predicted polypeptide revealed conserved structural features including ten repeats in the lipid-binding domain and some residues involved in cholesterol interaction and binding. The gene structure analysis identified four exons and three introns. Moreover, the synteny analysis revealed that apoA-I did not localize with other apolipoproteins indicating a divergent evolution with respect to the apoA-IV and apoE cluster...
May 2016: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/27258312/molecular-characterization-of-three-novel-phospholipase-a%C3%A2-proteins-from-the-venom-of-atheris-chlorechis-atheris-nitschei-and-atheris-squamigera
#17
He Wang, Xiaole Chen, Mei Zhou, Lei Wang, Tianbao Chen, Chris Shaw
Secretory phospholipase A₂ (sPLA₂) is known as a major component of snake venoms and displays higher-order catalytic hydrolysis functions as well as a wide range of pathological effects. Atheris is not a notoriously dangerous genus of snakes although there are some reports of fatal cases after envenomation due to the effects of coagulation disturbances and hemorrhaging. Molecular characterization of Atheris venom enzymes is incomplete and there are only a few reports in the literature. Here, we report, for the first time, the cloning and characterization of three novel cDNAs encoding phospholipase A₂ precursors (one each) from the venoms of the Western bush viper (Atheris chlorechis), the Great Lakes bush viper (Atheris nitschei) and the Variable bush viper (Atheris squamigera), using a "shotgun cloning" strategy...
2016: Toxins
https://www.readbyqxmd.com/read/27237594/liquid-chromatography-mass-spectrometry-determination-of-perfluoroalkyl-acids-in-environmental-solid-extracts-after-phospholipid-removal-and-on-line-turbulent-flow-chromatography-purification
#18
M Mazzoni, S Polesello, M Rusconi, S Valsecchi
An on-line TFC (Turbulent Flow Chromatography) clean up procedures coupled with UHPLC-MS/MS (Ultra High Performance Liquid Chromatography Mass Spectrometry) multi-residue method was developed for the simultaneous determination of 8 perfluroalkyl carboxylic acids (PFCA, from 5 to 12 carbon atoms) and 3 perfluoroalkyl sulfonic acids (PFSA, from 4 to 8 carbon atoms) in environmental solid matrices. Fast sample preparation procedure was based on a sonication-assisted extraction with acetonitrile. Phospholipids in biological samples were fully removed by an off-line SPE purification before injection, using HybridSPE(®) Phospholipid Ultra cartridges...
July 1, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27207954/synchronous-activity-lowers-the-energetic-cost-of-nest-escape-for-sea-turtle-hatchlings
#19
Mohd Uzair Rusli, David T Booth, Juanita Joseph
A potential advantage of group movement in animals is increased locomotion efficiency. This implies a reduced energetic cost for individuals that occur in larger groups such as herds, flocks and schools. When chelonian hatchlings hatch in the underground nest with finite energy for their post-hatching dispersal phase, they face the challenge of minimizing energetic expenditure while escaping the nest. The term 'social facilitation' has been used to describe the combined digging effort of sea turtle hatchlings during nest escape...
May 15, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27192057/molecular-characterization-and-function-analysis-of-the-vitellogenin-receptor-from-the-cotton-bollworm-helicoverpa-armigera-h%C3%A3-bner-lepidoptera-noctuidae
#20
Wanna Zhang, Long Ma, Haijun Xiao, Bingtang Xie, Guy Smagghe, Yuyuan Guo, Gemei Liang
Developing oocytes accumulate plentiful yolk protein during oogenesis through receptor-mediated endocytosis. The vitellogenin receptor (VgR), belonging to the low-density lipoprotein receptor (LDLR) family, regulates the absorption of yolk protein. In this work, the full-length vitellogenin receptor (HaVgR) in the cotton bollworm Helicoverpa armigera was identified, encoding a 1817 residue protein. Sequence alignment revealed that the sequence of HaVgR contained all of the conservative structural motifs of LDLR family members, and phylogenetic analysis indicated that HaVgR had a high identity among Lepidoptera and was distinct from that of other insects...
2016: PloS One
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