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Ovarian cryopreservation

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https://www.readbyqxmd.com/read/28065417/assisted-reproduction-technique-outcomes-for-fresh-versus-deferred-cryopreserved-day-2-embryo-transfer-a-retrospective-matched-cohort-study
#1
Mathilde Bourdon, Pietro Santulli, Vanessa Gayet, Chloé Maignien, Louis Marcellin, Khaled Pocate-Cheriet, Charles Chapron
Ovarian stimulation could adversely affect endometrial receptivity and consequently embryo implantation. One emerging strategy is the 'freeze-all' approach. Most studies have focused on blastocyst transfers, with limited research on day-2 deferred cryopreserved embryo transfers. In this large retrospective cohort study, outcomes were compared between day-2 fresh versus deferred cryopreserved embryo transfers. After matching by age and number of previous cycles, 325 cycles were included in the fresh group and 325 in the deferred cryopreserved embryo transfers group: no significant differences were found between groups in implantation (0...
December 19, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28063961/zinc-supplementation-of-vitrification-medium-improves-in-vitro-maturation-and-fertilization-of-oocytes-derived-from-vitrified-warmed-mouse-ovaries
#2
Shirin Geravandi, Mehri Azadbakht, Mahsa Pourmoradi, Fatemeh Nowrouzi
Oocyte cryopreservation is an approach for fertility preservation for normal women and cancer patients facing chemo and radiotherapy. The present study evaluated the effect of adding zinc chloride to the vitrification medium used for whole mouse ovaries and then assessing the in vitro maturation and fertilization of oocytes when they were subsequently extracted from these vitrified ovarian tissues. Four vitrification solutions with 0, 100,150 and 200 μg/dl zinc (V0, V1, V2 and V3 respectively) were compared...
January 4, 2017: Cryobiology
https://www.readbyqxmd.com/read/28050963/live-births-after-polar-body-biopsy-and-frozen-thawed-cleavage-stage-embryo-transfer-case-report
#3
Fernando Guimarães, Matheus Roque, Marcello Valle, Alessandra Kostolias, Rodrigo A de Azevedo, Ciro D Martinhago, Marcos Sampaio, Selmo Geber
Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050959/fertility-preservation-in-endometrial-cancer-is-it-safe-review-of-the-literature
#4
REVIEW
Márcia Mendonça Carneiro, Rívia Mara Lamaita, Márcia Cristina França Ferreira, Agnaldo Lopes Silva-Filho
Almost 5% of women with endometrial cancer are under age 40, and they often have well-differentiated endometrioid estrogen-dependent tumors. Cancer survival rates have improved over the last decades so strategies to avoid or reduce the reproductive damage caused by oncologic treatment are needed. We reviewed the published literature to find evidence to answer the following questions: How should we manage women in reproductive age with endometrial cancer? How safe is fertility preservation in endometrial cancer? Can pregnancy influence endometrial cancer recurrence? What are the fertility sparing options available? Progestins may be prescribed after careful evaluation and counseling...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28028773/86-successful-births-and-9-ongoing-pregnancies-worldwide-in-women-transplanted-with-frozen-thawed-ovarian-tissue-focus-on-birth-and-perinatal-outcome-in-40-of-these-children
#5
Annette Klüver Jensen, Kirsten Tryde Macklon, Jens Fedder, Erik Ernst, Peter Humaidan, Claus Yding Andersen
PURPOSE: This study aims to make an account of the children born following transplantation of frozen-thawed ovarian tissue worldwide with specific focus on the perinatal outcome of the children. Furthermore, perinatal outcome of seven deliveries (nine children) from Denmark is reported. METHODS: PubMed was searched for papers of deliveries resulting from ovarian tissue cryopreservation (OTC). Seven women underwent OTC prior to chemotherapy. Four of these women still had low ovarian function and had tried to conceive...
December 27, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28009430/timing-should-no-longer-be-an-obstacle-to-oocyte-cryopreservation-in-patients-with-cancer
#6
Giorgia Mangili, Enrico Papaleo, Cristina Sigismondi, Rossella Masciangelo, Veronica Sarais, Veronica Giorgione, Roberta Nale, Laura Privitera, Letizia Carnelli, Valentina Di Mattei, Massimo Candiani
PURPOSE: Anticancer treatment-related infertility is preventable with oocyte cryopreservation, but this is often not considered a relevant issue, due to lack of knowledge and time. The aim of this study is to prove that adequate organization of an Oncofertility Unit and the use of new protocols for controlled ovarian stimulation (COS) can reduce the time required by the procedure, encouraging consultants and patients to preserve fertility before gonadotoxic treatments. METHODS: A total of 125 patients diagnosed with malignant tumors were referred to the Oncofertility Unit of San Raffaele Hospital: 52 patients between April 2011 and October 2013 and 73 patients between October 2013 and November 2015...
November 28, 2016: Tumori
https://www.readbyqxmd.com/read/27999119/transgender-men-s-experiences-of-fertility-preservation-a-qualitative-study
#7
G Armuand, C Dhejne, J I Olofsson, K A Rodriguez-Wallberg
STUDY QUESTION: How do transgender men experience fertility preservation (FP) by cryopreservation of oocytes? SUMMARY ANSWER: The procedures required prior to oocyte cryopreservation, such as hormonal ovarian stimulation and transvaginal ultrasound (TVS), have a negative impact on gender dysphoria as they are closely linked to the men's female assigned sex at birth, which is incompatible with their current status. WHAT IS KNOWN ALREADY: Transgender persons often have high dissatisfaction with assigned sex-specific body features, such as the genital organs and androgen/oestrogen-responsive features...
December 19, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27986819/there-is-no-evidence-that-the-time-from-egg-retrieval-to-embryo-transfer-affects-live-birth-rates-in-a-freeze-all-strategy
#8
K Lattes, M A Checa, R Vassena, M Brassesco, V Vernaeve
STUDY QUESTION: Does the time from ovum pick-up (OPU) to frozen embryo transfer (FET) affect reproductive outcomes in a freeze-all strategy? SUMMARY ANSWER: Our study did not detect statistically significant differences between first and subsequent cycles, clinically relevant differences are not ruled out and further and larger studies are required. WHAT IS KNOWN ALREADY: Following controlled ovarian hyperstimulation (COH) delaying FET until the endometrium has returned to an optimal pre-stimulation state may have a significant emotional impact on patients, which adds to the stress and anxiety accompanying a standard IVF cycle...
December 16, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27981878/xenogeneic-decellularized-scaffold-a-novel-platform-for-ovary-regeneration
#9
Wen-Yue Liu, Shi-Gang Lin, Ru-Yi Zhuo, Yuan-Yuan Xie, Wei Pan, Xian-Feng Lin, Fei-Xia Shen
Women younger than 40 years may face early menopause because of premature ovarian failure (POF). The cause of POF can be idiopathic or iatrogenic, especially the cancer-induced oophorectomy and chemo- or radiation therapy. The current treatments, including hormone replacement therapy (HRT) and cryopreservation techniques, have increased risk of ovarian cancer and may reintroduce malignant cells after autografting. Decellularization technique has been regarded as a novel regenerative medicine strategy for organ replacement, wherein the living cells of an organ are removed, leaving the extracellular matrix (ECM) for cellular seeding...
January 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27960135/impact-of-gnrha-pretreatment-on-the-autotransplatation-efficacy-of-cytopreserved-rat-ovarian-tissue
#10
Xinhui Zhou, Weihua He, Rong Zhu, Yulong Zheng
Pre-therapeutic cryopreservation of ovarian tissue and subsequent transplantation after disease remission has recently emerged as an option to restore endocrinal function and preserve fertility for patients subjected to gonadotoxic treatments such as chemotherapy. However, the relatively low survival rate of follicles after grafting the frozen-thawed ovarian tissue largely limited the application of this technique. Gonadotropin-releasing hormone analogues (GnRHa), owing to their endocrinal regulatory effect, have been successfully used for fertility preservation against gonadotoxic conditions such as cytotoxic agents based anti-cancer therapy...
December 10, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27942575/in-vitro-maturation-and-cryopreservation-of-oocytes-retrieved-from-intra-operative-aspiration-during-second-enucleation-for-ovarian-tumor-a-case-report
#11
Hiromitsu Shirasawa, Yukiyo Kumazawa, Wataru Sato, Natsuki Ono, Yukihiro Terada
•We reported oocyte collection from an ovarian tumor with a single ovary.•Intra-operative retrieval of oocytes may be useful for preserving fertility.•We have done in vitro maturation for immature oocytes with ovarian enucleation.
February 2017: Gynecologic Oncology Reports
https://www.readbyqxmd.com/read/27925004/appropriate-osmotic-balance-duration-for-different-volumes-of-ovarian-tissue-in-vitrification-solution-a-study-of-ovary-tissue-vitrification-and-transplantation-in-sheep
#12
L Dang, X Zheng, Q Chang, Y Yang, Y Wang, Y Cai, C Hei, H Wang, C Zhao, W Zhu, Y Wang
:   BACKGROUND: Auto-transplantation of cryopreserved ovarian tissue has become a promising method for fertility preservation and standardization of the process is crucial for practical applications. OBJECTIVE: Here we used different size of large sheep ovarian cortex to study the most suitable osmotic balance durations in the vitrification solution for large piece ovary cortex. MATERIALS AND METHODS: The ovarian cortices from six-month old female sheep were divided into 40 cubic mm, 80 cubic mm and 160 cubic mm volume, A two-step osmotic balance method was used based on the results from morphological and histological study, we detected the expression of VEGF after thawing, the percentage of follicles that survived and serum E2 levels,together with apoptosis test by TUNEL...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27923859/non-growing-follicle-density-is-increased-following-adriamycin-bleomycin-vinblastine-and-dacarbazine-abvd-chemotherapy-in-the-adult-human-ovary
#13
M McLaughlin, T W Kelsey, W H B Wallace, R A Anderson, E E Telfer
STUDY QUESTION: Do the chemotherapeutic regimens of ABVD (adriamycin, bleomycin, vinblastine and dacarbazine) or OEPA-COPDAC (combined vincristine, etoposide, prednisone, doxorubicin (OEPA) and cyclophosphamide, vincristine, prednisone, dacarbazine (COPDAC)) used to treat Hodgkin lymphoma (HL), affect the density, morphology and in vitro developmental potential of human ovarian follicles? SUMMARY ANSWER: Ovarian tissue from women treated with ABVD contained a higher density of non-growing follicles (NGFs) per cubic millimetre and increased numbers of multiovular follicles but showed reduced in vitro growth compared with patients with lymphoma who had not received chemotherapy, patients treated with OEPA-COPDAC, age-matched healthy women and age-related model-predicted values...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27917673/-first-attempts-in-the-introduction-of-cryopreservation-of-ovarian-tissues
#14
Péter Fancsovits, János Urbancsek, László Fónyad, Anna Sebestyén, Gézáné Csorba, Ádám Lehner, Zita Kaszás, János Rigó, Attila Bokor
INTRODUCTION: The oncological treatment may damage ovarian function. To prevent this, it is possible to cryopreserve the ovarian tissue, and to keep the samples for long-term storage. The frozen-thawed tissue could be retransplanted after chemo- or radiotherapy. AIM: The aim of our study was to examine the effect of cryopreservation on the viability of ovarian tissue. METHOD: We analyzed the survival of frozen-thawed donated ovarian tissues...
December 2016: Orvosi Hetilap
https://www.readbyqxmd.com/read/27906588/fertility-preservation-in-pediatric-and-adolescent-oncology-patients-the-decision-making-process-of-parents
#15
Nancy Li, Yasmin Jayasinghe, Matthew A Kemertzis, Paddy Moore, Michelle Peate
PURPOSE: Decisions surrounding fertility preservation (FP) in children, adolescents, and adults can be difficult due to the distress of a cancer diagnosis, time constraints for decision-making, and lack of efficacy data. This review examines the decision-making process of oncology patients and their parents (if patients are in the pediatric or adolescent population) to better understand experiences of decisional conflict and regret. METHODS: Two electronic databases, Embase and Pubmed, were searched using the terms (Decision-making OR Conflict (Psychology) OR Decision regret) AND (Freezing OR Oocyte OR Ovarian tissue OR Semen preservation OR Fertility preservation OR Cryopreservation) AND (Neoplasms OR Cancer OR Chemotherapy OR Drug therapy OR Radiotherapy)...
December 1, 2016: Journal of Adolescent and Young Adult Oncology
https://www.readbyqxmd.com/read/27904170/twenty-five-year-old-woman-with-bilateral-borderline-ovarian-tumour-desiring-to-preserve-fertility-case-report-and-literature-review-on-the-current-state-of-fertility-preservation-in-women-with-borderline-ovarian-tumours
#16
S Findeklee, L Lotz, K Heusinger, I Hoffmann, R Dittrich, M W Beckmann
Borderline ovarian tumours are semimalignant tumours occurring unilaterally or bilaterally with a peak incidence among women of reproductive age. Since the affected women often wish to preserve fertility, particular precautions must be taken when counselling the patient and obtaining consent prior to planning an individual treatment. Options for preserving fertility include an organ-sparing surgical procedure and cryopreservation of oocytes and/or ovarian tissue. In this article, we report on a 25-year-old patient with a bilateral seromucinous borderline tumour who desired all fertility-preserving options...
November 2016: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/27892637/distribution-of-follicles-in-canine-ovarian-tissues-and-xenotransplantation-of-cryopreserved-ovarian-tissues-with-even-distribution-of-follicles
#17
I Wakasa, M Hayashi, Y Abe, H Suzuki
Ovarian follicles are not homogeneously distributed within the ovarian cortex in several species of mammals. Yet to maximize the reproducibility of experimental results of ovarian transplantation, it is essential to assess the degree of density and distribution of follicles in ovarian tissues before their transplantation. In this study, the ovarian cortex from 13 immature bitches (ten purebred and three mongrels) was sectioned into 1.0- to 1.5-mm(3) cubes, those were fixed, sectioned and stained with haematoxylin and eosin...
November 28, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27882769/-current-issues-of-reproductive-medicine-in-the-czech-republic
#18
P Ventruba, J Žáková, M Ješeta, D Rumpík, M Brandejská, I Crha, D Hlinka, L Jelínková, E Oráčová, J Pavelková, K Řežábek, K Veselá
INTRODUCTION: During the 25th symposium of assisted reproduction in Brno was lunch time organised as the lunch table discussion on the selected topics of assisted reproduction. More than 150 specialists reviewed themes related to gynecology and embryology.Discussed topics: Lunch table discussion covered the following topics: (1) Cross-border health care in assisted reproduction; (2) Indication for PGS (preimplantation genetic screening) in the context of actual information; (3) Does ovarian stimulation belong to the ambulance of registering gynecologists? (4) Therapy with clomifen - only for IVF specialists? (5) How and with whom should psychological support be directed during IVF? (6) Stimulation in women with low ovarian reserve; (7) Is basic semen analyses sufficient? (8) Time-lapse systems as relevant markers of embryonic development; (9) How to be oriented with choices of media and consumables in the IVF lab, and (10) "Freeze All" - is this new trend in cryopreservation suitable for all? CONCLUSIONS: Panel conclusions were presented during the afternoon session, which had great attendance, featured lively commentary, and produced some definitive consensus...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27870915/effects-of-angiopoietin-2-on-transplanted-mouse-ovarian-tissue
#19
Hye Won Youm, Jaewang Lee, Eun Jung Kim, Hyun Sun Kong, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Transplantation of ovarian tissue (OT) is currently the only clinical option to restore fertility with cryopreserved OT. However, follicle loss caused by ischemia and slow revascularization occurs in transplanted OT. To shorten the ischemic period and promote angiogenesis, some angiogenic factors have been used. Angiopoietin-2 (Ang2) is one of the major angiogenic factors and has been reported to promote blood vessels and increase vascular permeability in ischemic and/or hypoxic environment. This study was performed to investigate the effects of Ang2 on follicle integrity and revascularization of transplanted mouse OT...
2016: PloS One
https://www.readbyqxmd.com/read/27865446/the-use-of-utrogestan-during-controlled-ovarian-hyperstimulation-in-normally-ovulating-women-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection-treatments-in-combination-with-a-freeze-all-strategy-a-randomized-controlled-dose-finding-study
#20
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To compare the clinical characteristics in a Utrogestan and hMG protocol with the use of different doses of Utrogestan in normally ovulating women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatments. DESIGN: Prospective controlled study. SETTING: Tertiary-care academic medical center. PATIENT(S): A total of 150 infertile patients undergoing IVF/ICSI treatments. INTERVENTION(S): Utrogestan and hMG were administered simultaneously beginning on cycle day 3...
November 16, 2016: Fertility and Sterility
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