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Ovarian cryopreservation

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https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#1
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28510115/application-of-decellularized-tissue-scaffolds-in-ovarian-tissue-transplantation
#2
Enes Taylan, Kutluk Oktay
In tissue engineering, decellularized scaffolds have been proved to have remarkable capacity to promote regeneration in various organs such as kidney, heart, lung, and liver. Marrying the field of cryobiology and reproductive medicine resulted in considerable progress and breakthroughs, which led to the emergence of ovarian tissue cryopreservation and transplantation as a promising option for fertility preservation. Here we describe an innovative application of decellularized tissue scaffolds as a regenerative platform for reconstruction of ovarian grafts for auto-transplantation...
May 17, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28506797/-systematic-proposal-of-fertility-preservation-by-mature-oocyte-cryopreservation-for-recurrent-benign-ovarian-tumors
#3
Y Dadoun, H Azaïs, L Keller, E d'Orazio, P Collinet, C Decanter
OBJECTIVES: To investigate prospectively the pattern of the follicular growth and to characterize the COH outcome in terms of oocyte number and maturity in patients with voluminous recurrent benign ovarian tumors with a high surgical risk of significant reduction of the ovarian follicular content. METHODS: The inclusion criteria were: age between 18 and 36, presence of at least one benign ovarian tumor (≥ 5cm) with high risk of recurrence. The fertility preservation cycle was performed at least 3 months after the cyst surgery...
May 12, 2017: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/28502527/effects-of-different-sucrose-concentrations-on-vitrified-porcine-preantral-follicles-qualitative-and-quantitative-analysis
#4
Paula Rocío Gabriel, Pablo Torres, María Celeste Fratto, Humberto Cisale, Juan Alberto Claver, Daniel Marcelo Lombardo, María Laura Fischman
The aim of the present study was to perform a qualitative and quantitative analysis of the effect of different sucrose concentrations combined with ethylene glycol in the preservation of vitrified porcine preantral follicles. Fragments of ovarian cortex were vitrified in cryotubes containing 200 μl of the vitrification solution (30% Ethylene Glycol; 20% Fetal Bovine Serum; 0 M-0.25 M - 0.75 M or 1 M sucrose) and stored in liquid nitrogen for a week. Histological analysis showed that after vitrification the number of normal follicles decreased compared to the fresh tissue (control)...
May 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/28501366/reproductive-outcomes-after-a-single-dose-of-gonadotropin-releasing-hormone-agonist-compared-with-human-chorionic-gonadotropin-for-the-induction-of-final-oocyte-maturation-in-hyper-responder-women-aged-35-40%C3%A2-years
#5
Samer Tannus, Rola Turki, Yoni Cohen, Weon-Young Son, Tal Shavit, Michael Haim Dahan
OBJECTIVE: To investigate the reproductive outcomes after the use of GnRH agonist (GnRHa) compared with hCG for the induction of final oocyte maturation in GnRH antagonist cycles performed in hyper-responder women aged 35-40 years. DESIGN: Retrospective study. SETTING: Academic fertility center. PATIENT(S): Two hundred seventy-two hyper-responder women aged 35-40 years who underwent controlled ovarian stimulation under GnRH antagonist suppression were included...
May 10, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28486360/ovarian-mucinous-cystadenoma-after-ovarian-graft
#6
Carole Fajau-Prevot, Yann Tanguy Le Gac, Christine Chevreau, Clémentine Cohade, Nicolas Gatimel, Jean Parinaud, Roger Leandri
BACKGROUND: Freezing strips of ovarian cortex before chemotherapy followed by transplantation is an experimental method to preserve fertility for reproductive-aged women with cancer. We report a case of a cancer patient who developed a mucinous cystadenoma in a grafted piece of ovarian cortex. CASE: A 32-year-old woman with a Ewing sarcoma had ovarian cryopreservation using cortical strip freezing before receiving chemotherapy. Five years later she had no ovarian function, and the strips were thawed and grafted back onto the ovary...
May 5, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28467767/impact-of-endometrial-preparation-protocols-for-frozen-embryo-transfer-on-live-birth-rates
#7
Maria Cerrillo, Leyre Herrero, Alfredo Guillén, Mercedes Mayoral, Juan Antonio García-Velasco
BACKGROUND: It has been reported that a natural cycle (NC) is similar to or even better than hormone replacement therapy (HRT) in patients with regular cycles who undergo frozen embryo transfer (FET). Hundreds of FETs are managed yearly in our clinic. Scheduling these cycles is critical in a busy unit like ours. This is why we have to prove if a NC really shows a better outcome than other endometrium preparation protocols. METHODS: Hence we carried out a prospective study between June 2011 and June 2012, which included 530 patients (570 FET cycles) randomly allocated to two study groups: Group 1 (n=280 cycles), artificial cycle (HRT); or group 2 (n=290 cycles), natural cycle...
April 28, 2017: Rambam Maimonides Medical Journal
https://www.readbyqxmd.com/read/28459598/practices-and-attitudes-regarding-women-undergoing-fertility-preservation-a-survey-of-the-national-physicians-cooperative
#8
Pietro Bortoletto, Rafael Confino, Brigid M Smith, Teresa K Woodruff, Mary Ellen Pavone
PURPOSE: To describe physician attitudes and variations in oncofertility treatment strategies. METHODS: An exploratory online survey administered between December 1, 2014 and January 27, 2015 to 185 members of the National Physicians Cooperative (NPC). RESULTS: Twenty-eight percent (52 of 185) of NPC members responded to the online survey. Fifty percent of respondents were obstetrician-gynecologists working largely in academic medical centers...
May 1, 2017: Journal of Adolescent and Young Adult Oncology
https://www.readbyqxmd.com/read/28458301/ultrastructural-characteristics-of-human-oocytes-vitrified-before-and-after-in-vitro-maturation
#9
Yolanda Segovia, Noemí Victory, Irene Peinado, Laura M García-Valverde, Magdalena García, Jon Aizpurua, Ana Monzó, María José Gómez-Torres
The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MII oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM)...
April 30, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28455751/national-trends-and-outcomes-of-autologous-in-vitro-fertilization-cycles-among-women-ages-40-years-and-older
#10
Heather Hipp, Sara Crawford, Jennifer F Kawwass, Sheree L Boulet, David A Grainger, Dmitry M Kissin, Denise Jamieson
PURPOSE: The purpose of the study was to describe trends in and investigate variables associated with clinical pregnancy and live birth in autologous in vitro fertilization (IVF) cycles among women ≥40 years. METHODS: We used autologous IVF cycle data from the National ART Surveillance System (NASS) for women ≥40 years at cycle start. We assessed trends in fresh and frozen cycles (n = 371,536) from 1996 to 2013. We reported perinatal outcomes and determined variables associated with clinical pregnancy and live birth in fresh cycles between 2007 and 2013...
April 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28442066/characterisation-and-cryopreservation-of-the-ovarian-preantral-follicle-population-from-spix-s-yellow-toothed-cavies-galea-spixii-wagler-1831
#11
Érica C G Praxedes, Gabriela L Lima, Andréia M Silva, Carlos A C Apolinário, José A B Bezerra, Ana L P Souza, Moacir F Oliveira, Ana P R Rodrigues, Alexandre R Silva
The aim of the present study was to characterise the ovarian preantral follicle (PF) population and to establish a solid surface vitrification (SSV) process using dimethyl sulfoxide (DMSO) as a cryoprotectant for preservation of ovarian tissue from yellow-toothed cavies (Galea spixii). Ovaries were fixed for PF population analysis or were subjected to the SSV process. The mean (± s.e.m.) PF population per ovarian pair was estimated to be 416.0±342.8. There were 140.0±56.0 (63.4%) and 125.0±58.0 (64.0%) primary follicles on the right and left ovaries, respectively...
March 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28438562/first-successful-vitrification-of-salmonid-ovarian-tissue
#12
Jelena Lujić, Zoran Marinović, Simona Sušnik Bajec, Ida Djurdjevič, Eszter Kása, Béla Urbányi, Ákos Horváth
Due to a lack of cryopreservation protocols for fish eggs and embryos, alternative techniques which will enable storage of female genetic resources are crucial for future development of reproduction management in conservation biology and aquaculture. Experiments were conducted to develop an optimal vitrification protocol for cryopreservation of brown trout Salmo trutta juvenile ovarian tissue. Needle immersed vitrification (NIV) method was used where ovaries were pinned on an acupuncture needle, passaged through equilibration and vitrification solutions containing different combinations and concentrations of methanol (MeOH), propylene glycol (PG) and dimethyl sulfoxide (Me2SO) and subsequently plunged into liquid nitrogen...
April 21, 2017: Cryobiology
https://www.readbyqxmd.com/read/28433373/preimplantation-genetic-diagnosis-for-aneuploidy-testing-in-women-older-than-44-years-a-multicenter-experience
#13
Filippo Maria Ubaldi, Danilo Cimadomo, Antonio Capalbo, Alberto Vaiarelli, Laura Buffo, Elisabetta Trabucco, Susanna Ferrero, Elena Albani, Laura Rienzi, Paolo E Levi Setti
OBJECTIVE: To report laboratory and clinical outcomes in preimplantation genetic diagnosis for aneuploidies (PGD-A) cycles for women 44 to 47 years old. DESIGN: Multicenter, longitudinal, observational study. SETTING: In vitro fertilization (IVF) centers. PATIENT(S): One hundred and thirty-seven women aged 44.7 ± 0.7 years (range: 44.0-46.7) undergoing 150 PGD-A cycles during April 2013 to January 2016. INTERVENTION(S): Quantitative polymerase chain reaction-based PGD-A on trophectoderm biopsies and cryopreserved euploid single-embryo transfer (SET)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433369/fertility-preservation-and-refreezing-of-transplanted-ovarian-tissue-a-potential-new-way-of-managing-patients-with-low-risk-of-malignant-cell-recurrence
#14
Stine Gry Kristensen, Veronica Giorgione, Peter Humaidan, Birgit Alsbjerg, Anne-Mette Bay Bjørn, Erik Ernst, Claus Yding Andersen
OBJECTIVE: To report the first successful refreezing of ovarian tissue recovered more than 3 years after transplantation in a woman previously treated for early-stage ovarian cancer. DESIGN: Evaluation of cryopreserved and grafted ovarian tissue. SETTING: University hospital. PATIENT(S): A 23-year-old woman diagnosed with stage 1C ovarian mucinous cystadenocarcinoma. INTERVENTION(S): The patient underwent ovarian tissue cryopreservation for fertility preservation and subsequent heterotopic transplantation for fertility restoration 9 years after freezing...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28421499/chapter-15-development-of-a-nationwide-network-for-ovarian-tissue-cryopreservation
#15
Jana Liebenthron, Markus Montag
Ovarian tissue cryopreservation is gaining much interest since the publication of the first live birth after retransplantation of frozen-thawed tissue in 2004 (Donnez et al., Lancet 364:1405-1410, 2004). In contrast to cryopreservation of gametes and embryos, ovarian tissue freezing is a complex requiring a proper approach in order to make this a viable option for fertility preservation of cancer patients. Due to the need in terms of laboratory space, equipment, personnel, and adequate logistics, an ovarian tissue cryobank is most economic if managed as a centralized service unit that interacts with numerous clinics covering the surgical part...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421496/chapter-12-human-ovarian-tissue-slow-freezing
#16
Debra A Gook
Cryopreservation of human ovarian tissue is now being accepted as a mainstream ART laboratory procedure. The procedure described has been validated for use at the histological and functional level, and, recently, unequivocal evidence of preservation of primordial follicles has been demonstrated following twin births from cryopreserved ovarian tissue grafted at a heterotopic site.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421488/chapter-4-role-of-antioxidants-and-antifreeze-proteins-in-cryopreservation-vitrification
#17
Seul Ki Kim, Hye Won Youm, Jung Ryeol Lee, Chang Suk Suh
In recent years, supplementation of antioxidants and antifreeze proteins during cryopreservation/vitrification has significantly improved the survival and function of oocytes and ovarian tissues (OT) in animal models. In this chapter, the experimental protocols for the use of antioxidants and antifreeze proteins in cryopreservation/vitrification are described.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421486/chapter-2-utility-of-animal-models-for-human-ovarian-tissue-cryopreservation
#18
Seul Ki Kim, Jung Ryeol Lee, S Samuel Kim
Success in cryopreservation of ovarian tissue (OT) in animal models has led to develop efficient cryo-technologies for human ovarian tissue. In this chapter, cryopreservation protocols developed for animal experiments are described.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28419412/eliminating-malignant-cells-from-cryopreserved-ovarian-tissue-is-possible-in-leukaemia-patients
#19
Michelle Soares, Pascale Saussoy, Mathilde Maskens, Hélène Reul, Christiani A Amorim, Jacques Donnez, Marie-Madeleine Dolmans
Reimplantation of cryopreserved ovarian tissue (OT) can successfully restore ovarian function in young cancer patients after gonadotoxic treatment. However, for patients with leukaemia, there is a risk of malignant cell transmission. Our objective was to evaluate minimal disseminated disease in OT from leukaemia patients and test a follicle isolation technique to obtain disease-free follicle suspensions. Cryopreserved OT from 12 leukaemia patients was thawed and analysed by histology and long-term xenografting in immunosuppressed mice...
April 17, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28416291/cumulative-live-birth-rate-after-gnrh-agonist-trigger-and-elective-cryopreservation-of-all-embryos-in-high-responders
#20
Veljko Vlaisavljević, Borut Kovačič, Jure Knez
Elective embryo cryopreservation after using gonadotrophin-releasing hormone (GnRH) antagonist protocols and GnRH agonist triggering is becoming an increasingly important part of medically assisted reproduction. We designed a single-centre retrospective study to assess the cumulative probability of achieving a live birth through consecutive transfers of vitrified-warmed blastocysts after elective embryo cryopreservation in high-responding patients. Hence, 123 women identified to be at high risk for developing ovarian hyperstimulation syndrome were included...
April 7, 2017: Reproductive Biomedicine Online
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