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Ovarian cryopreservation

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https://www.readbyqxmd.com/read/28647785/fertility-preservation-for-trans-men-frozen-thawed-in-vitro-matured-oocytes-collected-at-the-time-of-ovarian-tissue-processing-exhibit-normal-meiotic%C3%A2-spindles
#1
S Lierman, K Tilleman, K Braeckmans, K Peynshaert, S Weyers, G T'Sjoen, P De Sutter
PURPOSES: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary...
June 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28647355/random-start-ovarian-stimulation-in-women-desiring-elective-cryopreservation-of-oocytes
#2
Nigel Pereira, Anna Voskuilen-Gonzalez, Kolbe Hancock, Jovana P Lekovich, Glenn L Schattman, Zev Rosenwaks
The current study investigates the utility of random-start ovarian stimulation in women desiring elective oocyte cryopreservation. Women in the study cohort underwent random-start ovarian stimulation, and were subdivided based on the phase of the menstrual cycle that ovarian stimulation began, i.e. early follicular, late follicular or luteal phase. Women undergoing conventional cycle day (CD) 2/3 ovarian stimulation start were controls. A total of 1302 women were included - 859 (66.0%) conventional CD 2/3, 342 (26...
June 12, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28646900/human-umbilical-cord-stem-cell-conditioned-medium-versus-serum-free-culture-medium-in-the-treatment-of-cryopreserved-human-ovarian-tissues-in-in-vitro-culture-a-randomized-controlled-trial
#3
Yingxian Jia, Xiaohan Shi, Yidong Xie, Xiaochuan Xie, Yan Wang, Shangwei Li
BACKGROUND: To reduce young female fertility loss, the in-vitro culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function...
June 24, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28645837/residual-ethylene-glycol-and-dimethyl-sulphoxide-concentration-in-human-ovarian-tissue-during-warming-thawing-steps-following-cryopreservation
#4
Yusuke Nakamura, Ryuichiro Obata, Noriyuki Okuyama, Nobuya Aono, Tomoko Hashimoto, Koichi Kyono
There have been 60 births after transplantation of cryopreserved ovarian tissue: 58 using the slow freezing method, and two using the vitrification method. DMSO and EG are widely used as cryoprotectants. However DMSO is a known epimutagen, and EG has been reported to be toxic in high concentrations. In this study, we measured residual DMSO and EG in ovarian tissue after vitrification and slow freezing. Cryoprotectants remained at a high concentration in the vitrified/warmed ovarian tissue just before transplantation (DMSO: 9...
June 6, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28645304/validation-of-an-automated-technique-for-ovarian-cortex-dissociation-isolation-of-viable-ovarian-cells-and-their-qualification-by-multicolor-flow-cytometry
#5
Tristan Zver, Elodie Mouloungui, Aurélie Berdin, Christophe Roux, Clotilde Amiot
BACKGROUND: Ovarian tissue cryopreservation is a technique for fertility preservation addressed to prepubertal girls or to patients for whom no ovarian stimulation is possible before initiation of gonadotoxic treatments. Autotransplantation of frozen-thawed ovarian tissue is the only available option for reuse but presents some limitations: ischemic tissue damages post-transplant and reintroduction of malignant cells in cases of cancer. It is therefore essential to qualify ovarian tissue before autograft on a functional and oncological point of view...
June 23, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28639180/initial-maternal-serum-human-chorionic-gonadotropin-levels-in-pregnancies-achieved-after-assisted-reproductive-technology-are-higher-after-preimplantation-genetic-screening-and-after-frozen-embryo-transfer-a-retrospective-cohort
#6
Elie Hobeika, Sonali Singh, Shaveta Malik, Eric S Knochenhauer, Michael L Traub
PURPOSE: Few published articles have compared initial hCG values across all different types of ART cycles, including cycles with fresh or frozen embryo transfer. No articles have compared initial hCG values in cycles utilizing preimplantation genetic screening (PGS). The purpose of this study is to compare initial hCG values after fresh embryo transfer, frozen embryo transfer, and after PGS. METHODS: This was a single-center retrospective cohort study at an academically affiliated private IVF center...
June 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28629583/ex%C3%A2-vivo-retrieval-and-cryopreservation-of-oocytes-from-oophorectomized-specimens-for-fertility-preservation-in-a-brca1-mutation-carrier-with-ovarian-cancer
#7
Nigel Pereira, Andrea G Hubschmann, Jovana P Lekovich, Glenn L Schattman, Zev Rosenwaks
OBJECTIVE: To report a case of ex vivo oocyte retrieval from oophorectomized specimens in a BRCA1 mutation carrier undergoing surgical staging for ovarian cancer. DESIGN: Video case report and literature review. SETTING: University-affiliated center. PATIENT(S): A 37-year-old single woman, gravida 0, with a known BRCA1 mutation, presented to her oncologist with a complex right ovarian mass and elevated CA-125 level...
June 16, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28609269/corifollitropin-alfa-compared-to-daily-rfsh-or-hp-hmg-in-gnrh-antagonist-controlled-ovarian-stimulation-protocol-for-patients-undergoing-assisted-reproduction
#8
Priscila Morais Galvão Souza, Bruno Ramalho de Carvalho, Hitomi Miura Nakagawa, Thalita Reis Esselin Rassi, Antônio César Paes Barbosa, Adelino Amaral Silva
OBJECTIVE: This study aimed to compare the outcomes of controlled ovarian stimulation (COS) with corifollitropin alfa versus daily recombinant follicle-stimulating hormone (rRFSH) or highly purified human menopausal gonadotropin (HP-HMG) in patients undergoing in vitro fertilization (IVF) cycles based on gonadotropin-releasing hormone (GnRH) antagonist protocols. The primary endpoints were total number of oocytes and mature oocytes. METHODS: This retrospective study looked into 132 controlled ovarian stimulation cycles from IVF or oocyte cryopreservation performed in a private human reproduction center between January 1 and December 31, 2014...
June 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28601378/gnrh-agonist-administration-prior-to-embryo-transfer-in-freeze-all-cycles-of-patients-with-endometriosis-or-aberrant-endometrial-integrin-expression
#9
Eric S Surrey, Mandy Katz-Jaffe, Laxmi V Kondapalli, Robert L Gustofson, William B Schoolcraft
Prolonged gonadotrophin-releasing hormone agonist (GnRHa) administration before IVF with fresh embryo transfer to patients with endometriosis or aberrant endometrial integrin expression (-integrin) improves outcomes but may suppress ovarian response and prevents elective cryopreservation of all embryos. This retrospective cohort pilot study evaluates freeze-all cycles with subsequent prolonged GnRHa before embryo transfer in these populations. Patients from 2010 to 2015 who met inclusion criteria and received a long-acting GnRHa every 28 days twice before FET were evaluated...
May 17, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28590419/melatonin-and-fertoprotective-adjuvants-prevention-against-premature-ovarian-failure-during-chemotherapy
#10
REVIEW
Hoon Jang, Kwonho Hong, Youngsok Choi
Premature ovarian failure is one of the side effects of chemotherapy in pre-menopausal cancer patients. Preservation of fertility has become increasingly important in improving the quality of life of completely recovered cancer patients. Among the possible strategies for preserving fertility such as ovarian tissue cryopreservation, co-treatment with a pharmacological adjuvant is highly effective and poses less of a burden on the human body. Melatonin is generally produced in various tissues and acts as a universally acting antioxidant in cells...
June 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28583597/equine-ovarian-tissue-viability-after-cryopreservation-and-in%C3%A2-vitro-culture
#11
G D A Gastal, F L N Aguiar, B G Alves, K A Alves, S G S de Tarso, G M Ishak, C A Cavinder, J M Feugang, E L Gastal
Ovarian tissue cryopreservation allows the preservation of the female fertility potential for an undetermined period. The objectives of this study were to compare the efficiency of cryoprotective agents (CPAs; dimethyl sulfoxide, DMSO; ethylene glycol, EG; and propylene glycol, PROH) using slow-freezing and vitrification methods, and evaluate the viability of cryopreserved equine ovarian tissue after 7 days of culture. Fresh and cryopreserved ovarian fragments were evaluated for preantral follicle morphology, stromal cell density, EGFR, Ki-67, Bax, and Bcl-2 protein expression, and DNA fragmentation...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28580514/complete-protection-against-cryodamage-of-cryopreserved-whole-bovine-and-human-ovaries-using-dmso-as-a-cryoprotectant
#12
Johan R Westphal, Renne Gerritse, Didi D M Braat, Catharina C M Beerendonk, Ronald Peek
PURPOSE: This study aims to determine the optimal cryopreservation protocol for whole ovaries intended for preservation of fertility in women. METHODS: We investigated the optimal cryopreservation procedure for whole ovaries in a bovine model. The following parameters were investigated to determine their effect on ovarian tissue viability: type of cryoprotectant, administration route of the cryoprotectant (perfusion and/or submersion), and the maximum tolerable interval between death of the animal and start of the cryopreservation process...
June 3, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28576861/pharmacological-inhibition-of-the-dna-damage-checkpoint-prevents-radiation-induced-oocyte-death
#13
Vera D Rinaldi, Kristin Hsieh, Robert Munroe, Ewelina M Bolcun-Filas, John C Schimenti
Ovarian function is directly correlated with survival of the primordial follicle reserve. Women diagnosed with cancer have a primary imperative of treating the cancer, but since the resting oocytes are hypersensitive to the DNA-damaging modalities of certain chemo- and radiotherapeutic regimens, such patients face the collateral outcome of premature loss of fertility and ovarian endocrine function. Current options for fertility preservation primarily include collection and cryopreservation of oocytes or in vitro fertilized oocytes, but this necessitates a delay in cancer treatment and additional assisted reproductive technology (ART) procedures...
June 2, 2017: Genetics
https://www.readbyqxmd.com/read/28558117/gonadotropin-releasing-hormone-agonist-triggering-and-a-freeze-all-approach-the-final-step-in-eliminating-ovarian-hyperstimulation-syndrome
#14
Marcus J Davenport, Beverley Vollenhoven, Alon J Talmor
Importance: In vitro fertilization (IVF) has evolved dramatically in recent decades; however, clinical practices have been slow to adopt these advancements, particularly regarding final oocyte maturation and the timing of embryo transfer. Concerns still exist over the ability of gonadotropin-releasing hormone (GnRH) agonists and elective embryo cryopreservation to reduce the risk of ovarian hyperstimulation syndrome (OHSS) without compromising pregnancy outcomes. Objective: This review investigates IVF outcomes associated with GnRH-agonist triggering and elective embryo cryopreservation...
May 2017: Obstetrical & Gynecological Survey
https://www.readbyqxmd.com/read/28550713/state-of-the-art-on-oocyte-cryopreservation-in-female-cancer-patients-a-critical-review-of-the-literature
#15
REVIEW
Claudia Massarotti, Paola Scaruffi, Matteo Lambertini, Valentino Remorgida, Lucia Del Mastro, Paola Anserini
During the last decades, important advances in therapeutic options have led to increased survival rates in cancer patients; however, cancer treatments are associated with several potential adverse effects including infertility in those diagnosed during their reproductive years. A proper discussion about fertility preservation options before the use of therapies with potential gonadotoxicity (i.e. oncofertility counseling) is standard of care and should be offered to all patients of childbearing age. Temporary ovarian suppression with LH-RH analogs, oocyte and embryo cryopreservation are standard strategies for fertility preservation in female cancer patients...
June 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28534057/the-effect-of-thawing-protocols-on-follicle-conservation-in-human-ovarian-tissue-cryopreservation
#16
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28528388/fertility-sparing-for-young-patients-with-gynecologic-cancer-how-mri-can-guide-patient-selection-prior-to-conservative-management
#17
REVIEW
Sinead H McEvoy, Stephanie Nougaret, Nadeem R Abu-Rustum, Hebert Alberto Vargas, Elizabeth A Sadowski, Christine O Menias, Fuki Shitano, Shinya Fujii, Ramon E Sosa, Joanna G Escalon, Evis Sala, Yulia Lakhman
Historically, cancer treatment has emphasized measures for the "cure" regardless of the long-term consequences. Advances in cancer detection and treatment have resulted in improved outcomes bringing to the fore various quality of life considerations including future fertility. For many young cancer patients, fertility preservation is now an integral component of clinical decision-making and treatment design. Optimal fertility-sparing options for young patients with gynecologic cancer are influenced by patient age, primary cancer, treatment regimens, and patient preferences...
May 20, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#18
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28510115/application-of-decellularized-tissue-scaffolds-in-ovarian-tissue-transplantation
#19
Enes Taylan, Kutluk Oktay
In tissue engineering, decellularized scaffolds have been proved to have remarkable capacity to promote regeneration in various organs such as kidney, heart, lung, and liver. Marrying the field of cryobiology and reproductive medicine resulted in considerable progress and breakthroughs, which led to the emergence of ovarian tissue cryopreservation and transplantation as a promising option for fertility preservation. Here we describe an innovative application of decellularized tissue scaffolds as a regenerative platform for reconstruction of ovarian grafts for auto-transplantation...
May 17, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28506797/-systematic-proposal-of-fertility-preservation-by-mature-oocyte-cryopreservation-for-recurrent-benign-ovarian-tumors
#20
Y Dadoun, H Azaïs, L Keller, E d'Orazio, P Collinet, C Decanter
OBJECTIVES: To investigate prospectively the pattern of the follicular growth and to characterize the COH outcome in terms of oocyte number and maturity in patients with voluminous recurrent benign ovarian tumors with a high surgical risk of significant reduction of the ovarian follicular content. METHODS: The inclusion criteria were: age between 18 and 36, presence of at least one benign ovarian tumor (≥ 5cm) with high risk of recurrence. The fertility preservation cycle was performed at least 3 months after the cyst surgery...
May 12, 2017: Gynecologie, Obstetrique, Fertilite & Senologie
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