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https://www.readbyqxmd.com/read/27931714/host-plant-growth-promotion-and-cadmium-detoxification-in-solanum-nigrum-mediated-by-endophytic-fungi
#1
Abdur Rahim Khan, Ihsan Ullah, Muhammad Waqas, Gun-Seok Park, Abdul Latif Khan, Sung-Jun Hong, Rehman Ullah, Byung Kwon Jung, Chang Eon Park, Shafiq Ur-Rehman, In-Jung Lee, Jae-Ho Shin
Current investigation conducted to evaluate the associated fungal endophyte interactions of a Cd hyper-accumulator Solanum nigrum Korean ecotype under varying concentrations of Cd. Two indole-3-acetic acid (IAA) producing fungal strains, RSF-4L and RSF-6L, isolated from the leaves of S. nigrum, were initially screened for Cd tolerance and accumulation potential. In terms of dry biomass production, the strain RSF-6L showed higher tolerance and accumulation capacity for Cd toxicity in comparison to RSF-4L. Therefore, RSF-6L was applied in vivo to S...
February 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27929414/pb-2-effects-on-growth-lipids-and-protein-and-dna-profiles-of-the-thermophilic-bacterium-thermus-thermophilus
#2
Barbara Nicolaus, Annarita Poli, Paola Di Donato, Ida Romano, Giusi Laezza, Alessia Gioiello, Sergio Ulgiati, Florinda Fratianni, Filomena Nazzaro, Pierangelo Orlando, Stefano Dumontet
Extremophiles are organisms able to thrive in extreme environmental conditions and some of them show the ability to survive high doses of heavy metals thanks to defensive mechanisms provided by primary and secondary metabolic products, i.e., extremolytes, lipids, and extremozymes. This is why there is a growing scientific and industrial interest in the use of thermophilic bacteria in a host of tasks, from the environmental detoxification of heavy metal to industrial activities, such as bio-machining and bio-metallurgy...
December 6, 2016: Microorganisms
https://www.readbyqxmd.com/read/27928781/mechanism-of-fluorescence-enhancement-of-biosynthesized-xfe2o4-bifeo3-x-cr-mn-co-or-ni-membranes
#3
Liang Bian, Hai-Long Li, Hai-Liang Dong, Fa-Qin Dong, Mian-Xin Song, Li-Sheng Wang, Wen-Ping Hou, Lei Gao, Xiao-Yan Zhang, Tian-Liang Zhou, Guang-Ai Sun, Xin-Xi Li, Lei Xie
Ferrites-bismuth ferrite is an intriguing option for medical diagnostic imaging device due to its magnetoelectric and enhanced near-infrared fluorescent properties. However, the embedded XFO nanoparticles are randomly located on the BFO membranes, making implementation in devices difficult. To overcome this, we present a facile bio-approach to produce XFe2O4-BiFeO3 (XFO-BFO) (X = Cr, Mn, Co, or Ni) membranes using Shewanella oneidensis MR-1. The perovskite BFO enhances the fluorescence intensity (at 660 and 832 nm) and surface potential difference (-469 ~ 385 meV and -80 ~ 525 meV) of the embedded spinel XFO...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27927915/comparative-evaluation-of-four-phenotypic-tests-for-the-detection-of-carbapenemase-producing-gram-negative-bacteria
#4
Audrey Noël, Te-Din Huang, Catherine Berhin, Martin Hoebeke, Warda Bouchahrouf, Sami Yunus, Pierre Bogaerts, Youri Glupczynski
Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates i.e. the Neo-Rapid CARB kit (Rosco Diagnostica A/S), the RAPIDEC CARBA NP test (BioMérieux SA), the β CARBA test (Bio-Rad Laboratories N.V) and a home-made electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n= 198], nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27927161/scifio-an-extensible-framework-to-support-scientific-image-formats
#5
Mark C Hiner, Curtis T Rueden, Kevin W Eliceiri
BACKGROUND: No gold standard exists in the world of scientific image acquisition; a proliferation of instruments each with its own proprietary data format has made out-of-the-box sharing of that data nearly impossible. In the field of light microscopy, the Bio-Formats library was designed to translate such proprietary data formats to a common, open-source schema, enabling sharing and reproduction of scientific results. While Bio-Formats has proved successful for microscopy images, the greater scientific community was lacking a domain-independent framework for format translation...
December 7, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/27925410/unraveling-the-role-of-formic-acid-and-the-type-of-solvent-in-the-catalytic-conversion-of-lignin-a-holistic-approach
#6
Mikel Oregui Bengoechea, Iñaki Gandarias, Pedro Luis Arias, Tanja Barth
The role of formic acid together with the effect of the solvent type and their synergic interactions with a NiMo catalyst were studied for the conversion of lignin into bio-oil in an alcohol/formic acid media. Replacing formic acid with molecular hydrogen or isopropanol decreased the oil yield to a considerable degree, increased the solid yield and altered the nature of the bio-oil. The differences induced by the presence of molecular hydrogen were comparable to the ones observed in the isopropanol system, suggesting similar lignin conversion mechanisms for both systems...
December 7, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27925173/potential-of-long-non-coding-rnas-in-cancer-patients-from-bio-markers-to-therapeutic-targets
#7
REVIEW
Subash Chandra Gupta, Yashoda Nandan Tripathi
Because of high specificity and easy detection in the tissues, serum, plasma, urine and saliva, interest in exploring the potential of long non-coding RNAs (lncRNAs) in cancer patients continues to increase. LncRNAs have shown potential as a bio-marker in the diagnosis and prognosis of bladder cancer, prostate cancer, gastric cancer, pancreatic cancer, breast cancer, and many other cancer types. Some lncRNAs have also been used as adjunct to improve the specificity and sensitivity of existing biomarkers. The molecular tools such as RNA-seq, RNA-FISH, ic-SHAPE and quantitative real-time PCR have been used for examining the lncRNAs' potential...
December 7, 2016: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27924784/bio-fabrication-of-nanomesh-channels-of-single-walled-carbon-nanotubes-for-locally-gated-field-effect-transistors
#8
Hye-Hyeon Byeon, Woo Chul Lee, Wonbin Kim, Seong Keun Kim, Woong Kim, Hyunjung Yi
Single-walled carbon nanotubes (SWNTs) are one of the promising electronic components for nanoscale electronic devices such as field-effect transistors (FETs) owing to their excellent device characteristics such as high conductivity, high carrier mobility and mechanical flexibility. Localized gating gemometry of FETs enables individual addressing of active channels and allows for better electrostatics via thinner dielectric layer of high k-value. For localized gating of SWNTs, it becomes critical to define SWNTs of controlled nanostructures and functionality onto desired locations in high precision...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27924490/site-specific-fluorescent-labeling-of-argonaute-for-fret-based-bio-assays
#9
Sarah Willkomm, Adrian Zander, Dina Grohmann
Deciphering the molecular mechanisms of eukaryotic Argonaute proteins is crucial for the understanding of RNA interference (RNAi), a posttranscriptional gene silencing process. Fluorescence-based single-molecule studies like single-molecule Förster resonance energy transfer (FRET) between a donor and acceptor dye represent a versatile tool to gain a mechanistic understanding of the structural dynamics of a biomolecular complex. Until today it was not possible to site-specifically introduce fluorophores into eukaryotic Argonaute...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924256/operating-principles-of-rotary-molecular-motors-differences-between-f1-and-v1-motors
#10
REVIEW
Ichiro Yamato, Yoshimi Kakinuma, Takeshi Murata
Among the many types of bioenergy-transducing machineries, F- and V-ATPases are unique bio- and nano-molecular rotary motors. The rotational catalysis of F1-ATPase has been investigated in detail, and molecular mechanisms have been proposed based on the crystal structures of the complex and on extensive single-molecule rotational observations. Recently, we obtained crystal structures of bacterial V1-ATPase (A3B3 and A3B3DF complexes) in the presence and absence of nucleotides. Based on these new structures, we present a novel model for the rotational catalysis mechanism of V1-ATPase, which is different from that of F1-ATPases...
2016: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/27922209/unconventional-pretreatment-of-lignocellulose-with-low-temperature-plasma
#11
Jens Vanneste, Thijs Ennaert, Annick Vanhulsel, Bert F Sels
Lignocellulose represents a potential supply of sustainable feedstock for the production of biofuels and chemicals. There is, however, an important cost and efficiency challenge associated with the conversion of such lignocellulosics. Since its structure is complex and not prone to undergo chemical reactions very easily, chemical and mechanical pretreatments are usually necessary in order to be able to refine them into the compositional building blocks (carbohydrates and lignin), from which value-added platform molecules such as glucose, ethylene glycol, 5-hydroxymethylfurfural, levulinic acid, etc...
December 6, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27922072/molecular-structure-and-dynamics-of-water-on-pristine-and-strained-phosphorene-wetting-and-diffusion-at-nanoscale
#12
Wei Zhang, Chao Ye, Linbi Hong, Zaixing Yang, Ruhong Zhou
Phosphorene, a newly fabricated two-dimensional (2D) nanomaterial, has emerged as a promising material for biomedical applications with great potential. Nonetheless, understanding the wetting and diffusive properties of bio-fluids on phosphorene which are of fundamental importance to these applications remains elusive. In this work, using molecular dynamics (MD) simulations, we investigated the structural and dynamic properties of water on both pristine and strained phosphorene. Our simulations indicate that the diffusion of water molecules on the phosphorene surface is anisotropic, with strain-enhanced diffusion clearly present, which arises from strain-induced smoothing of the energy landscape...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27918446/metabolomics-and-its-application-in-the-development-of-discovering-biomarkers-for-osteoporosis-research
#13
REVIEW
Huanhuan Lv, Feng Jiang, Daogang Guan, Cheng Lu, Baosheng Guo, Chileung Chan, Songlin Peng, Baoqin Liu, Wenwei Guo, Hailong Zhu, Xuegong Xu, Aiping Lu, Ge Zhang
Osteoporosis is a progressive skeletal disorder characterized by low bone mass and increased risk of fracture in later life. The incidence and costs associated with treating osteoporosis cause heavy socio-economic burden. Currently, the diagnosis of osteoporosis mainly depends on bone mineral density and bone turnover markers. However, these indexes are not sensitive and accurate enough to reflect the osteoporosis progression. Metabolomics offers the potential for a holistic approach for clinical diagnoses and treatment, as well as understanding of the pathological mechanism of osteoporosis...
December 2, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27918290/nacre-mimetic-bulk-lamellar-composites-reinforced-with-high-aspect-ratio-glass-flakes
#14
Selen N Gurbuz Guner, Arcan F Dericioglu
Nacre-mimetic epoxy matrix composites reinforced with readily available micron-sized high aspect ratio C-glass flakes were fabricated by a relatively simple, single-step, scalable, time, cost and man-power effective processing strategy: hot-press assisted slip casting (HASC). HASC enables the fabrication of preferentially oriented two-dimensional inorganic reinforcement-polymer matrix bulk lamellar composites with a micro-scale structure resembling the brick-and-mortar architecture of nacre. By applying the micro-scale design guideline found in nacre and optimizing the relative volume fractions of the reinforcement and the matrix as well as by anchoring the brick-and-mortar architecture, and tailoring the interface between reinforcements and the matrix via silane coupling agents, strong, stiff and tough bio-inspired nacre-mimetic bulk composites were fabricated...
December 5, 2016: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/27915023/anisotropic-noble-metal-nanoparticles-synthesis-surface-functionalization-and-applications-in-biosensing-bioimaging-drug-delivery-and-theranostics
#15
REVIEW
Gokul Paramasivam, Namitharan Kayambu, Arul Maximus Rabel, Ashok K Sundramoorthy, Anandhakumar Sundaramurthy
Anisotropic nanoparticles have fascinated scientists and engineering communities for over a century because of their unique physical and chemical properties. In recent years, continuous advances in design and fabrication of anisotropic nanoparticles have opened new avenues for application in various areas of biology, chemistry and physics. Anisotropic nanoparticles have the plasmon absorption in the visible as well as near-infrared (NIR) region, which enable them to be used for crucial applications such as biological imaging, medical diagnostics and therapy ("theranostics")...
November 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27911420/-role-of-immunological-factors-in-the-development-of-myopic-choroidal-neovascularization
#16
A G Shchuko, N V Zaitseva, T N Yur'eva, E R Chernykh, I M Mikhalevich, A V Grigor'eva
AIM: To study the concentration of cytokines in the aqueous humor of the anterior chamber in patients with myopic choroidal neovascularization (mCNV) and to compare the results to their ophthalmic status. MATERIAL AND METHODS: A total of 19 patients (19 eyes) with mCNV treated with intravitreal ranibizumab were included in the study. The control group consisted of 15 patients (15 eyes) with myopia who had cataract surgery. Age, sex, and refractive error distribution were similar to that in the study group...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27910541/research-on-the-best-measurement-situation-between-optical-probe-and-tissue-surfaces-in-non-invasive-detection
#17
Xuyao Yu, Rong Liu, Hui Yu, Jiao Wang, Jun Wang, Kexin Xu
Near-infrared spectroscopy is often used for the non-invasive detection of composition in the human body, such as that of blood glucose and haemoglobin, due to its high penetration depth into tissues. Although it is feasible to position the optical probe precisely, contact situation between probe and human tissues is a difficult problem to determine because of physiological tremor and mechanical performance of bio-soft tissue. Here, we proposed a novel estimation method for the situation between the optical probe and tissue surfaces based on the dynamic auto-correlation matrix of two-dimensional correlation spectroscopy (2DCOS) and radar chart...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909726/microrna%C3%A2-21-promotes-migration-and-invasion-of-glioma-cells-via-activation-of-sox2-and-%C3%AE-%C3%A2-catenin-signaling
#18
Guoxuan Luo, Wentao Luo, Xiaohui Sun, Jinzhi Lin, Mo Wang, Yang Zhang, Weishi Luo, Yong Zhang
The expression of microRNA 21 (miR-21) has been reported to be upregulated in various types of cancer, including malignant gliomas. However, its functions and mechanisms in glioma remain to be fully elucidated. The present study established miRNA‑21 overexpression and knockdown cell lines using SRY‑box 2 (Sox2) small interfering RNA (siRNA) to knockdown expression and Sox2 cDNA was cloned into pcDNA 3.1 mammalian expression vector for ectopic expression. BIO and XAV‑939 were used for β‑catenin signaling activation and knockdown, respectively...
November 28, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27909720/tetrandrine-inhibits-glioma-stem-like-cells-by-repressing-%C3%AE-catenin-expression
#19
Yong Zhang, Yu-Lin Wen, Ji-Wei Ma, Jie-Cheng Ye, Xiao Wang, Jian-Xian Huang, Chao-Yue Meng, Xiao-Ze Xu, Shao-Xiang Wang, Xue-Yun Zhong
Cancer stem cells (CSCs) in glioma are often responsible for relapse and resistance to therapy. The purpose of the present study was to confirm the self-renewal and migration inhibitory effects of tetrandrine (Tet), which is a compound extracted from the dried root of Stephania tetrandra S. Moore, toward glioma stem-like cells (GSLCs) and to examine the associated molecular mechanisms. Using a neurosphere culture technique, we enriched the GSLC population from the human glioblastoma cell lines U87 and U251...
November 23, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27908016/bistable-gaits-and-wobbling-induced-by-pedestrian-bridge-interactions
#20
Igor V Belykh, Russell Jeter, Vladimir N Belykh
Several modern footbridges around the world have experienced large lateral vibrations during crowd loading events. The onset of large-amplitude bridge wobbling has generally been attributed to crowd synchrony; although, its role in the initiation of wobbling has been challenged. To study the contribution of a single pedestrian into overall, possibly unsynchronized, crowd dynamics, we use a bio-mechanically inspired inverted pendulum model of human balance and analyze its bi-directional interaction with a lively bridge...
November 2016: Chaos
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