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https://www.readbyqxmd.com/read/28342550/the-effects-of-endothelin-1-on-satellite-glial-cells-in-peripheral-ganglia
#1
Rachel Feldman-Goriachnik, Menachem Hanani
Endothelins (ET) are a family of highly active neuropeptides with manifold influences via ET receptors (ETR) in both the peripheral and central nervous systems. We have shown previously that satellite glial cells (SGCs) in mouse trigeminal ganglia (TG) are extremely sensitive to ET-1 in evoking [Ca(2+)]in increase, apparently via ETBR activation, but there is no functional information on ETR in SGCs of other peripheral ganglia. Here we tested the effects of ET-1 on SGCs in nodose ganglia (NG), which is sensory, and superior cervical ganglia (Sup-CG), which is part of the sympathetic nervous system, and further investigated the influence of ET-1 on SGCs in TG...
March 18, 2017: Neuropeptides
https://www.readbyqxmd.com/read/28337973/crystal-growth-structure-and-magnetic-properties-of-ca-sub-10-sub-cr-sub-7-sub-o-sub-28-sub
#2
Christian Balz, Bella Lake, Manfred Reehuis, A T M Islam, Oleksandr Prokhnenko, Yogesh Singh, Philip Pattison, Sandor Toth
A detailed diffraction study of Ca<sub>10</sub>Cr<sub>7</sub>O<sub>28</sub> is presented which adds significant new insights into the structural and magnetic properties of this compound. A new crystal structure type was used where the a and b axes are doubled compared to previous models providing a more plausible structure where all crystallographic sites are fully occupied. The presence of two different valences of chromium was verified and the locations of the magnetic Cr<sup>5+</sup> and non-magnetic Cr<sup>6+</sup> ions were identified...
March 24, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28337641/inhibition-of-proliferation-of-prostate-cancer-cell-line-du-145-in-vitro-and-in-vivo-using-salvia-miltiorrhiza-bunge
#3
Woong Jin Bae, Jin Bong Choi, Kang Sup Kim, U Syn Ha, Sung Hoo Hong, Ji Youl Lee, Tae-Kon Hwang, Sung Yeoun Hwang, Zhi-Ping Wang, Sae Woong Kim
OBJECTIVE: To investigate the antiproliferative activity of Salvia miltiorrhiza Bunge. (SM) on the castration-resistant prostate cancer (CRPC) cell line DU-145, in vitro and in vivo. METHODS: Prostate cancer cell line (DU-145) and normal prostate cell line (RWPE-1) were treated with SM at different concentrations (3.125, 12.5, 25 and 50 μg/mL) to investigate the antiproliferative effects. DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells...
March 23, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28335768/leisure-sedentary-time-is-differentially-associated-with-hypertension-diabetes-mellitus-and-hyperlipidemia-depending-on-occupation
#4
Man Sup Lim, Bumjung Park, Il Gyu Kong, Songyong Sim, So Young Kim, Jin-Hwan Kim, Hyo Geun Choi
BACKGROUND: Sedentary behavior is considered an independent cause of cardio-metabolic diseases, regardless of physical activity level and obesity. Few studies have reported the association between leisure sedentary time and cardio-vascular diseases in terms of occupation. METHODS: We performed a cross-sectional study using data from the Korean Community Health Survey (KCHS) for 240,086 participants assessed in 2011 and 2013. Occupation was categorized into four groups: farmer or fisherman, laborer, and soldier (Group I); service worker, salesperson, technician, mechanic, production worker, and engineer (Group II); manager, expert, specialist, and clerk (Group III); and unemployed (Group IV)...
March 23, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28332486/fabrication-of-the-flexible-highly-ordered-porous-alumina-templates-by-the-combined-nanosphere-lithography-and-anodization
#5
Michal Krupinski, Marcin Perzanowski, Alexey Maximenko, Yevhen Zabila, Marta Marszalek
In this work, we propose a new method for a large-scale production of flexible, periodic alumina arrays with well-ordered pores. We show the incorporation of pre-patterning based on a polystyrene (PS) nanosphere lithography into aluminium anodization process. We prepared ordered monolayers of PS spheres with average diameters of (510 ± 10) nm, and (430 ± 10) nm on a large area (1.5 x 1.5 cm<sup>2</sup>) of the Si substrate. Next, we deposited the 5 μm aluminium layer on arrays of PS nanospheres using the sputtering technique...
March 23, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28331079/characterization-and-vaccine-potential-of-membrane-vesicles-produced-by-francisella-noatunensis-sup-orientalis-in-an-adult-zebrafish-model
#6
Leidy Lagos, Julia I Tandberg, Urska Repnik, Preben Boysen, Erik Ropstad, Deepa Varkey, Ian T Paulsen, Hanne C Winther-Larsen
Vaccine development against extracellular bacteria has been important for the sustainability of the aquaculture industry. In contrast, infections with intracellular pathogens remain largely an unresolved problem. Francisella noatunensis subspecies orientalis (Fno) are Gram-negative, facultative intracellular bacteria that cause the disease francisellosis in fish. Francisellosis is commonly characterized as a chronic granulomatous disease with high morbidity and can result in high mortality depending on the host...
March 22, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28330527/validation-of-multiresidue-method-for-analysis-of-31-pesticides-in-rice-using-gas-chromatography-tandem-mass-spectrometry
#7
Rahul Mondal, Ramen Kumar Kole, Anjan Bhattacharyya
Five modified QuEChERS were tested for the multiresidue analysis of 31 pesticides in rice. Rice was spiked with mixturesolution of pesticides at 10 ng/g. Method selection was based on the LODs (sensitivity) and recovery tests (accuracy) of the pesticides. Analysis was done in GC-tandem MS in multiple reaction monitoring mode with a total run time of approximately 37 min. The selected method was validated after spiking rice at 20 and 100 ng/g in rice. The performance characteristics of the method impacted for all selected pesticides were acceptable according to the guidelines for method validation (recovery of 70-120% with an RSD of <20% and r<sup>2</sup> value of ≥0...
February 22, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28327473/simulation-study-of-a-d-shape-pet-scanner-for-improved-sensitivity-and-reduced-cost-in-whole-body-imaging
#8
Abdella M Ahmed, Hideaki Tashima, Taiga Yamaya
Many research efforts are being made to increase the sensitivity and improve the imaging performance of positron emission tomography (PET) scanners. Conventionally, sensitivity can be increased by increasing the number of detector rings in the axial direction (but at high cost) or reduce the diameter of the scanner (with the disadvantages of reducing the space for patients and degrading the spatial resolution due to the parallax error). In this study, we proposed a PET scanner with a truncated ring and an array of detectors that can be arranged in a straight line below the bed...
March 22, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28323636/ultrasmall-water-dispersible-tween80-modified-yb-er-nagd-wo-sub-4-sub-sub-2-sub-nanoparticles-with-record-upconversion-ratiometric-thermal-sensitivity-and-their-internalization-by-mesenchymal-stem-cells
#9
Concepcion Cascales, Carlos Paino, Eulalia Bazán, Carlos Zaldo
This work presents the synthesis by coprecipitation of diamond shaped Yb:Er:NaGd(WO<sub>4</sub>)<sub>2</sub> crystalline nanoparticles (NPs) with diagonal dimensions in the 5-7 nm × 10-12 nm range which have been modified with TWEEN80 for their dispersion in water, and their interaction with mesenchymal stem cells (MSCs) proposed as cellular NP vehicles. These NPs belong to a large family of tetragonal Yb:Er:NaT(XO<sub>4</sub>)<sub>2</sub> (T=Y, La, Gd, Lu; X= Mo, W) compounds with green (<sup>2</sup>H<sub>11/2</sub>+<sup>4</sup>S<sub>3/2</sub>→<sup>4</sup>I<sub>15/2</sub>) Er-related upconversion (UC) efficiency comparable to that of Yb:Er:β-NaYF<sub>4</sub> reference compound, but with a ratiometric thermal sensitivity (S) 2...
March 21, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28322043/-sup-18-sup-f-fluoro-hydroxyphenethylguanidines-efficient-synthesis-and-comparison-of-two-structural-isomers-as-radiotracers-of-cardiac-sympathetic-innervation
#10
Yong-Woon Jung, Keun Sam Jang, Guie Gu, Robert A Koeppe, Phillip S Sherman, Carole A Quesada, David M Raffel
Fluorine-18 labeled phenethylguanidines are currently under development in our laboratory as radiotracers for quantifying regional cardiac sympathetic nerve density using PET imaging techniques. In this study, we report an efficient synthesis of <sup>18</sup>F-hydroxyphenethylguanidines consisting of nucleophilic aromatic [<sup>18</sup>F]fluorination of a protected diaryliodonium salt precursor followed by a single deprotection step to afford the desired radiolabeled compound. This approach has been shown to reliably produce 4-[<sup>18</sup>F]fluoro-<i>m</I>-hydroxyphenethylguanidine ([<sup>18</sup>F]4F-MHPG, [<sup>18</sup>F]<b>1</b>) and its structural isomer 3-[<sup>18</sup>F]fluoro-<i>p</i>-hydroxyphenethylguanidine ([<sup>18</sup>F]3F-PHPG, [<sup>18</sup>F]<b>2</b>) with good radiochemical yields...
March 21, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28315638/determination-of-triazines-and-triazoles-in-grapes-using-atmospheric-pressure-matrix-assisted-laser-desorption-ionization-high-resolution-mass-spectrometry
#11
Vishal Mahale, Ajeet Singh, Gayatri S Phadke, Avinash D Ghanate, Dasharath P Oulkar, Kaushik Banerjee, Venkateswarlu Panchagnula
A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ)...
March 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28315586/sucralfate-versus-histamine-2-receptor-antagonists-for-stress-ulcer-prophylaxis-in-adult-critically-ill-patients-a-meta-analysis-and-trial-sequential-analysis-of-randomized-trials
#12
Mustafa Alquraini, Fayez Alshamsi, Morten Hylander Møller, Emilie Belley-Cote, Saleh Almenawer, Roman Jaeschke, Robert MacLaren, Waleed Alhazzani
PURPOSE: To determine the impact of using sucralfate versus H2RAs for SUP on patient important outcomes. MATERIALS AND METHODS: We searched CENTRAL, MEDLINE, EMBASE, ACPJC, clinical trials registries, and conference proceedings through June 2016 for randomized controlled trials (RCTs) comparing sucralfate to H2RAs for SUP in adult critically ill patients. RESULTS: 21 RCTs enrolling 3121 patients met inclusion criteria. There was no significant difference between sucralfate compared to H2RAs in the risk of clinically important GI bleeding (risk ratio [RR] 1...
March 8, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28315293/phase-ii-trial-of-intensity-modulated-radiotherapy-concurrent-with-chemotherapy-for-postoperative-node-positive-esophageal-squamous-cell-carcinoma
#13
Hua Tao, Yiqin Zhou, Chengyun Yao, Dayong Gu, Wei Chen, Jincheng Lu
The aim of this article is to evaluate the efficacy and toxicity of intensity-modulated radiotherapy concurrent with weekly docetaxel in patients with node-positive esophageal squamous cell carcinoma after radical surgery. Between January 2011 and December 2013, a total of 46 eligible patients were planned for enrollment. All the patients received intensity-modulated radiotherapy concurrent with weekly docetaxel (20 mg/m<sup>2</sup>). Patients were treated 5 days per week at 2.0 Gy/d. The total dose of external radiotherapy given was 50 Gy in 25 fractions...
March 8, 2017: Oncology Research
https://www.readbyqxmd.com/read/28314559/corrigendum-to-morphologic-analysis-of-occipital-sinuses-for-occipital-screw-fixation-using-digital-subtraction-angiography-world-neurosurg-91-2016-279-284
#14
Dong Hoon Lee, Jae Taek Hong, Jae Hoon Sung, Ashish Jain, Joon Huh, Sang Uk Kim, Joon Young Kim, Jae Yoel Kwon, Chul Bum Cho, Il Sup Kim, Sang Won Lee
No abstract text is available yet for this article.
March 14, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28314113/genome-wide-estimation-of-heritability-and-its-functional-components-for-flowering-defense-ionomics-and-developmental-traits-in-a-geographically-diverse-population-of-i-arabidopsis-thaliana-i
#15
Rong-Cai Yang
Narrow-sense heritability (portion of the total phenotypic variation due to additive genetic effect, <i>h</i><sup>2</sup>) is a critical parameter in plant breeding and genetics, but its estimation is difficult for populations with unknown pedigree information. This study applied a marker-based linear mixed model (LMM) analysis to estimate narrow-sense heritability and its seven functional components corresponding to SNPs in coding and noncoding regions for each of 107 flowering, defense, ionomics and developmental traits in an Arabidopsis (<i>Arabidopsis thaliana</i>) population of 199 inbred lines with unknown genetic relatedness...
March 17, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28314108/-i-roegneria-alashanica-i-keng-a-species-with-the-b-ststst-sup-y-sup-st-sup-y-sup-b-genome-constitution
#16
Richard R-C Wang, Kevin B Jensen
The genome constitution of tetraploid <i>Roegneria alashanica</I> Keng has been in question for a long time. Most scientific studies have suggested that <i>R. alashanica</I> had two versions of the <b>St</b> genome, <b>St<sub>1</sub>St<sub>2</sub></b>, similar to that of <i>Pseudoroegneria elytrigioides</I> (C. Yen et J. L. Yang) B. R. Lu. A study, however, concluded that <i>R. alashanica</I> had the <b>StY</b> genome formula typical for tetraploid <i>Roegneria</I> species...
March 17, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28304172/ab-initio-studies-on-the-clathrate-hydrates-of-some-nitrogen-and-sulfur-containing-gases
#17
Ningru Sun, Zewen Li, Nianxiang Qiu, Xiaohui Yu, Xuran Zhang, Yanjun Li, Longbin Yang, Kan Luo, Qing Huang, Shiyu Du
Ab initio calculations are performed to investigate the host-guest interactions and multiple occupancies of some sulfur- (H2S, CS2) and nitrogen-containing (N2, NO, and NH3) molecules in dodecahedral, tetrakaidecahedral, and hexakaidecahedral water cages in this work. Five functionals in the framework of density functional theory are compared, and the M06-2X method appears to be the best to predict the binding energies as well as the geometries. Results show that N2 and NO molecules are more stable in the 5(12)6(4) cage, while NH3 and H2S prefer to stabilize in the 5(12)6(2) cage...
March 24, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28303785/fast-gas-chromatography-with-tandem-mass-spectrometry-analysis-of-selected-persistent-organic-pollutants-and-organophosphorus-and-synthetic-pyrethroid-pesticides-in-indian-prawn-fenneropenaeus-indicus-in-compliance-with-the-eu-mrls
#18
Durvesh Sawant, Jitendra Kelkar, Pratap Rasam, Ajit Datar, Prashant Hase, Dheeraj Handique, Ankush Bhone, Sanket Chiplunkar, Manish Hate
A fast GC with tandem MS method was developed and validated for multiresidue determination of 95 chemical contaminants(24 synthetic pyrethroids, 17 organochlorines, 17 organophosphorus compounds, 18 polycyclic aromatic hydrocarbons, and 19 polychlorinated biphenyls) in Indian prawns (<i>Fenneropenaeus indicus</i>) as per the European Union maximum residual limit requirements. Chromatographic separation and MS determination were achieved within a short run time of 18 min, without compromising sensitivity and specificity...
March 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28301492/variability-in-target-delineation-of-cervical-carcinoma-a-korean-radiation-oncology-group-study-krog-15-06
#19
Ji Hyeon Joo, Young Seok Kim, Byung Chul Cho, Chi Young Jeong, Won Park, Hak Jae Kim, Won Sup Yoon, Mee Sun Yoon, Ji-Yoon Kim, Jin Hwa Choi, Youngmin Choi, Joo-Young Kim
PURPOSE: To determine inter-observer variability in target volume definition of cervical cancer in radical and adjuvant radiotherapy (RT) settings. METHODS: Eight physicians contoured CTVs of 2 patients underwent definitive and postoperative RT. Each volume was analyzed using the individual/median volume ratio and generalized conformity index (CIgen). And center of mass (COM) of each contour was calculated. Expert agreement was quantified using an expectation maximization algorithm for Simultaneous Truth and Performance Level Estimation (STAPLE)...
2017: PloS One
https://www.readbyqxmd.com/read/28297686/-early-experience-with-the-vercise-sup-tm-sup-dbs-system-in-the-treatment-of-dystonic-tremor
#20
Yasushi Miyagi
Six cases of dystonic tremor were treated with the Vercise<sup>TM</sup> deep brain stimulation(DBS)system, which has the multiple independent current control(MICC)technology. The mean preoperative score of Burke-Fahn-Marsden dystonia rating scale was 16.2±9.4, which was reduced to 6.1±4.6 at 5 months postoperatively. A 65-year-old male presented an intractable dystonic tremor of the jaw, neck, and shoulders due to tardive syndrome. He experienced the successful tremor relief after unipolar DBS in the globus pallidus internus(GPi)with Vercise<sup>TM</sup> but complained of dysarthria...
March 2017: No Shinkei Geka. Neurological Surgery
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