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X-G Liu, J Xu, F Li, M-J Li, T Hu
OBJECTIVE: Chemoresistance is one of the obstacles for effective treatment of cancers, and recent evidence has shown that microRNAs play critical roles in drug resistance. In this study, we investigated the effect of miR-377 on cisplatin resistance in osteosarcoma (OS). PATIENTS AND METHODS: Tumor tissues from chemoresistant and control OS patients were subjected to Real-time polymerase chain reaction (PCR) to assess miR-377 expression. The effect and mechanism of miR-377 on cisplatin resistance were assessed using Cell Counting Kit (CCK) 8, flow cytometry, Western blot, and luciferase assays in cisplatin-resistant OS cells lines...
March 2018: European Review for Medical and Pharmacological Sciences
Emine Sekerdag, Ihsan Solaroglu, Yasemin Gursoy-Ozdemir
As a result of ischemia or hemorrhage, blood supply to neurons is disrupted which subsequently promotes a cascade of pathophysiological responses resulting in cell loss. Many mechanisms are involved solely or in combination in this disorder including excitotoxicity, mitochondrial death pathways, and the release of free radicals, protein misfolding, apoptosis, necrosis, autophagy and inflammation. Besides neuronal cell loss, damage to and loss of astrocytes as well as injury to white matter contributes also to cerebral injury...
March 20, 2018: Current Neuropharmacology
Christopher R Broda, Svetlana B Shugh, Rohan B Parikh, YunFei Wang, Tobias R Schlingmann, Cory V Noel
After an arterial switch operation (ASO), serial imaging is necessary to monitor for maladaptive changes. We compared cardiac magnetic resonance imaging (CMR) to 2-D transthoracic echocardiography (TTE) in assessing post-operative ASO patients. We performed a retrospective review of patients at a single tertiary care center who underwent an ASO and subsequently had a CMR performed from 7/2010 to 7/2016. Those with single ventricle anatomy, congenitally corrected transposition of the great arteries, or previous atrial switch operation were excluded...
March 22, 2018: Pediatric Cardiology
Hayder Hamzah, Ali Abduljabar, Jonathan Lees, Adrian Porch
A miniaturized 2.4 GHz re-entrant cavity has been designed, manufactured and tested as a sensor for microfluidic compositional analysis. It has been fully evaluated experimentally with water and common solvents, namely methanol, ethanol, and chloroform, with excellent agreement with the expected behaviour predicted by the Debye model. The sensor's performance has also been assessed for analysis of segmented flow using water and oil. The samples' interaction with the electric field in the gap region has been maximized by aligning the sample tube parallel to the electric field in this region, and the small width of the gap (typically 1 mm) result in a highly localised complex permittivity measurement...
March 19, 2018: Sensors
Gianvincenzo Sparacia, Roberto Cannella, Vincenzina Lo Re, Giuseppe Mamone, Koji Sakai, Kei Yamada, Roberto Miraglia
PURPOSE: To assess brain-core temperature of end-stage liver disease patients undergoing orthotopic liver transplantation (OLT) using a temperature measurement technique based on the apparent diffusion coefficient of the cerebrospinal fluid in the lateral ventricles. MATERIALS AND METHODS: The study group was composed of 19 patients with a model for end-stage liver disease (MELD) score of 23.7 who underwent MR imaging before and after OLT. MR imaging studies were performed with a 1...
March 19, 2018: Japanese Journal of Radiology
Syed M Tareq, M Tauhid Ur Rahman, A Z M Zahedul Islam, A B M Baddruzzaman, M Ashraf Ali
Climate-induced waterlogging has been significantly affecting the lives and livelihood of people in the south-west coastal region of Bangladesh for a couple of decades. The objective of this study is to investigate the waterlogging hazards of Tala, a south-western coastal Upazila of Bangladesh by analyzing satellite image. An empirical model based on velocity of flow, depth of flow, and inundation depth has been proposed to assess waterlogging hazard in pre monsoon, monsoon, and post monsoon period. Landsat TM images for the years 1989, 2000, and 2011 were analyzed by using Geoinformatics including GIS and remote sensing techniques to quantify the water hazard...
March 19, 2018: Environmental Monitoring and Assessment
Ulrike Binsker, Raghavendra Palankar, Jan Wesche, Thomas P Kohler, Josephine Prucha, Gerhard Burchhardt, Manfred Rohde, Frank Schmidt, Barbara M Bröker, Uwe Mamat, Jan Pané-Farré, Anica Graf, Patrick Ebner, Andreas Greinacher, Sven Hammerschmidt
Staphylococcus aureus can cause bloodstream infections associated with infective endocarditis (IE) and disseminated intravascular coagulopathy (DIC). Both complications involve platelets. In view of an increasing number of antibiotic-resistant strains, new approaches to control systemic S. aureus infection are gaining importance. Using a repertoire of 52 recombinant S. aureus proteins in flow cytometry-based platelet activation and aggregation assays, we identified, in addition to the extracellular adherence protein Eap, three secreted staphylococcal proteins as novel platelet activating proteins...
March 19, 2018: Thrombosis and Haemostasis
Jacqueline Cloos, Jeffrey R Harris, Jeroen J W M Janssen, Angele Kelder, F Huang, Gerrit Sijm, Maike Vonk, Alexander N Snel, Jennifer R Scheick, Willemijn J Scholten, Jannemieke Carbaat-Ham, Dennis Veldhuizen, Diana Hanekamp, Yvonne J M Oussoren-Brockhoff, Gertjan J L Kaspers, Gerrit J Schuurhuis, A Kate Sasser, Gert Ossenkoppele
Response criteria in acute myeloid leukemia (AML) has recently been re-established, with morphologic examination utilized to determine whether patients have achieved complete remission (CR). Approximately half of the adult patients who entered CR will relapse within 12 months due to the outgrowth of residual AML cells in the bone marrow. The quantitation of these remaining leukemia cells, known as minimal or measurable residual disease (MRD), can be a robust biomarker for the prediction of these relapses. Moreover, retrospective analysis of several studies has shown that the presence of MRD in the bone marrow of AML patients correlates with poor survival...
March 5, 2018: Journal of Visualized Experiments: JoVE
Marietta Zita Poles, Nikolett Bódi, Mária Bagyánszki, Éva Fekete, András Tamás Mészáros, Gabriella Varga, Szilárd Szűcs, Anna Nászai, Liliána Kiss, Andrey V Kozlov, Mihály Boros, József Kaszaki
Our aim was to characterize the main components of the nitrosative response with quantitative changes of the nitrergic myenteric neurons in adjacent intestinal segments after transient superior mesenteric artery occlusion. We also tested the hypothesis that exogenous methane may modulate the evolution of nitroxidation by influencing xanthine oxidoreductase (XOR) activity. The microcirculatory consequences of a 50min ischemia or ischemia-reperfusion were investigated in anesthetized rats (n=124) inhaling normoxic air with or without 2...
March 14, 2018: Free Radical Biology & Medicine
Yaowarin Nakornpakdee, Rasana W Sermswan, Santi Maneewatchararangsri, Surasakdi Wongratanacheewin
BACKGROUND: Leptospirosis is a bacterial disease caused by the Leptospira interrogans. The hamster is considered a susceptible host while the mouse is resistant. The knowledge of hamster T cell immunity is limited compared to the mouse. The reason why the hamster and the mouse give different responses to leptospires remains unclear. OBJECTIVE: To determine the differential responses of CD4+ T cells between hamsters and mice using Leptospira interrogans as an infectious model...
March 12, 2018: Asian Pacific Journal of Allergy and Immunology
Takahiro Nemoto, Alexandros Alexakis
The fluctuations of turbulence intensity in a pipe flow around the critical Reynolds number is difficult to study but important because they are related to turbulent-laminar transitions. We here propose a rare-event sampling method to study such fluctuations in order to measure the time scale of the transition efficiently. The method is composed of two parts: (i) the measurement of typical fluctuations (the bulk part of an accumulative probability function) and (ii) the measurement of rare fluctuations (the tail part of the probability function) by employing dynamics where a feedback control of the Reynolds number is implemented...
February 2018: Physical Review. E
Cheng Jin, Jianjiang Feng, Lei Wang, Heng Yu, Jiang Liu, Jiwen Lu, Jie Zhou
In this paper, we present an approach for left atrial appendage (LAA) multi-phase fast segmentation and quantitative assisted diagnosis of atrial fibrillation (AF) based on 4D-CT data. We take full advantage of the temporal dimension information to segment the living, flailed LAA based on a parametric max-flow method and graph-cut approach to build 3-D model of each phase. To assist the diagnosis of AF, we calculate the volumes of 3-D models, and then generate a "volume-phase" curve to calculate the important dynamic metrics: ejection fraction, filling flux, and emptying flux of the LAA's blood by volume...
March 9, 2018: Computers in Biology and Medicine
Sebastian V Nielsen, Martin Kellner, Per G Henriksen, Håkan Olsén, Steen H Hansen, Erik Baatrup
Selective serotonin re-uptake inhibitors are pharmaceuticals used to treat a range of psychological disorders. They are frequently found in surface waters in populated areas. In recent years, they have been shown to affect the behaviour of various aquatic organisms in a way that can have ecological effects. In this study, we exposed zebrafish of both sexes to nominally 0.00, 0.15 and 1.50 µg L-1 Escitalopram in flow-through tanks for three weeks. Subsequently, ten swimming behaviour parameters were quantified using high-resolution video tracking...
March 14, 2018: Ecotoxicology
Sho Tamai, Yasuhiko Hayashi, Yasuo Sasagawa, Masahiro Oishi, Mitsutoshi Nakada
Background: Although it is well known that most choroid plexus cysts (CPCs) are asymptomatic, previous studies have reported that they can infrequently cause progressive hydrocephalus along with their increasing sizes. Among those cases, some patients needed cyst fenestration or cerebrospinal fluid (CSF) diversion to recover neurological deterioration. Meanwhile, some CPCs revealed spontaneous resolution, and in rare cases, they developed re-accumulation. Some reports have described series of radiological findings about their changes in location...
2018: Surgical Neurology International
Collin D Edington, Wen Li Kelly Chen, Emily Geishecker, Timothy Kassis, Luis R Soenksen, Brij M Bhushan, Duncan Freake, Jared Kirschner, Christian Maass, Nikolaos Tsamandouras, Jorge Valdez, Christi D Cook, Tom Parent, Stephen Snyder, Jiajie Yu, Emily Suter, Michael Shockley, Jason Velazquez, Jeremy J Velazquez, Linda Stockdale, Julia P Papps, Iris Lee, Nicholas Vann, Mario Gamboa, Matthew E LaBarge, Zhe Zhong, Xin Wang, Laurie A Boyer, Douglas A Lauffenburger, Rebecca L Carrier, Catherine Communal, Steven R Tannenbaum, Cynthia L Stokes, David J Hughes, Gaurav Rohatgi, David L Trumper, Murat Cirit, Linda G Griffith
Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-system microfluidic platforms that are compatible with the quantitative study of a range of compounds, including lipophilic drugs. We describe three different platform designs - "4-way", "7-way", and "10-way" - each accommodating a mixing chamber and up to 4, 7, or 10 MPSs...
March 14, 2018: Scientific Reports
Jessica Hill, Johnathan Matlock, Quentin Lefebvre, Katie Cooper, Jonathan Clayden
Tetrahydroisoquinolines and tetrahydrobenzazepines are prepared by acid-promoted ring contraction of cyclic ureas, themselves formed by ring expansion of indolines and tetrahydroquinolines. The consequent overall one-carbon insertion reaction gives these 6- and 7-membered heterocyclic scaffolds in three steps from readily available precursors. Other ring sizes may be formed by an alternative elimination reaction of bicyclic structures. Scalability of the method was demonstrated by operating it in a flow system...
March 14, 2018: Angewandte Chemie
Hanh Ngo, Roberto Forero, David Mountain, Daniel Fatovich, Wing Nicola Man, Peter Sprivulis, Mohammed Mohsin, Sam Toloo, Antonio Celenza, Gerard Fitzgerald, Sally McCarthy, Ken Hillman
BACKGROUND: In 2009, the Western Australian (WA) Government introduced the Four-Hour Rule (FHR) program. The policy stated that most patients presenting to Emergency Departments (EDs) were to be seen and either admitted, transferred, or discharged within 4 hours. This study utilised de-identified data from five participating hospitals, before and after FHR implementation, to assess the impact of the FHR on several areas of ED functioning. METHODS: A state (WA) population-based intervention study design, using longitudinal data obtained from administrative health databases via record linkage methodology, and interrupted time series analysis technique...
2018: PloS One
Zhi Li, Elizabeth Gothard, Mark C Coles, Carrie A Ambler
In skin wounds, innate-immune cells clear up tissue debris and microbial contamination, and also secrete cytokines and other growth factors that impact repair process such as re-epithelialization and wound closure. After injury, there is a rapid influx and efflux of immune cells at wound sites, yet the function of each innate cell population in skin repair is still under investigation. Flow cytometry is a valuable research tool for detecting and quantifying immune cells; however, in mouse back skin, the difficulty in extracting immune cells from small area of skin due to tissue complexity has made cytometric analysis an underutilized tool...
2018: Frontiers in Immunology
Antoine Paccard, Ben A Wasserman, Dieta Hanson, Louis Astorg, Dan Durston, Sara Kurland, Travis M Apgar, Rana W El-Sabaawi, Eric P Palkovacs, Andrew P Hendry, Rowan D H Barrett
The evolutionary consequences of temporal variation in selection remain hotly debated. We explored these consequences by studying threespine stickleback in a set of bar-built estuaries along the central California coast. In most years, heavy rains induce water flow strong enough to break through isolating sand bars, connecting streams to the ocean. New sand bars typically re-form within a few weeks or months, thereby re-isolating populations within the estuaries. These breaching events cause severe and often extremely rapid changes in abiotic and biotic conditions, including shifts in predator abundance...
March 13, 2018: Journal of Evolutionary Biology
Radhouan El Zrelli, Lotfi Rabaoui, Nabil Daghbouj, Heithem Abda, Sylvie Castet, Claudie Josse, Pieter van Beek, Marc Souhaut, Sylvain Michel, Nejla Bejaoui, Pierre Courjault-Radé
Since the establishment of the coastal industrial complex in Gabes city (Gulf of Gabes, SE Tunisia), hundred million tons of untreated phosphogypsum have been discharged in the open sea causing serious environmental problems. To better understand the dynamic and behavior of phosphate/phosphogypsum contaminants from raw ores to marine environment, a chemical, organic, mineralogical, and morphological characterization of phosphate rock and phosphogypsum was conducted using several sophisticated techniques. The chemical analysis showed that phosphate and phosphogypsum contain high loads of trace elements and that the transfer factors of pollutants varied from 5...
March 12, 2018: Environmental Science and Pollution Research International
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