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https://www.readbyqxmd.com/read/28924656/live-cell-sted-imaging-with-the-hyper2-biosensor
#1
Natalia M Mishina, Vsevolod V Belousov
Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins of interest allows subdiffraction imaging of intracellular macromolecular architecture with simultaneous extraction of functional information about cellular activities. Here, we describe a protocol for live-cell STED microscopy of the HyPer2 biosensor fused to cytoskeletal filaments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28912561/luminophore-configuration-and-concentration-dependent-optoelectronic-characteristics-of-a-quantum-dot-embedded-dna-hybrid-thin-film
#2
Velu Arasu, Sreekantha Reddy Dugasani, Mallikarjuna Reddy Kesama, Ho Kyoon Chung, Sung Ha Park
To be useful in optoelectronic devices and sensors, a platform comprising stable fluorescence materials is essential. Here we constructed quantum dots (QDs) embedded DNA thin films which aims for stable fluorescence through the stabilization of QDs in the high aspect ratio salmon DNA (SDNA) matrix. Also for maximum luminescence, different concentration and configurations of core- and core/alloy/shell-type QDs were embedded within SDNA. The QD-SDNA thin films were constructed by drop-casting and investigated their optoelectronic properties...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28863030/persistent-super-utilization-of-acute-care-services-among-subgroups-of-veterans-experiencing-homelessness
#3
Dorota Szymkowiak, Ann Elizabeth Montgomery, Erin E Johnson, Todd Manning, Thomas P O'Toole
BACKGROUND: Acute health care utilization often occurs among persons experiencing homelessness. However, knowing which individuals will be persistent super-utilizers of acute care is less well understood. OBJECTIVE: The objective of the study was to identify those more likely to be persistent super-utilizers of acute care services. RESEARCH DESIGN: We conducted a latent class analysis of secondary data from the Veterans Health Administration Corporate Data Warehouse, and Homeless Operations Management and Evaluation System...
August 31, 2017: Medical Care
https://www.readbyqxmd.com/read/28861995/chemo-regio-and-stereoselective-n-alkenylation-of-pyrazoles-benzpyrazoles-using-activated-and-unactivated-alkynes
#4
Vineeta Garg, Pradeep Kumar, Akhilesh K Verma
Transition-metal-free chemo-, regio-, and stereoselective synthesis of (Z) and (E) styryl pyrazoles and benzpyrazoles by the addition of N-heterocycles onto functionalized terminal and internal alkynes using a super basic solution of KOH/DMSO has been described. The stereochemical outcome of the reaction was governed by time and quantity of the base. The reaction of pyrazoles and benzpyrazoles onto alkynes takes place chemoselectively without affecting the free -NH2 group of pyrazoles and -OH group of alkynes...
September 14, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28858629/selective-expression-of-the-transcription-elongation-factor-ell3-in-b-cells-prior-to-ell2-drives-proliferation-and-survival
#5
Lou-Ella M M Alexander, January Watters, Jessica A Reusch, Michelle Maurin, Brook S Nepon-Sixt, Katerina Vrzalikova, Mark G Alexandrow, Paul G Murray, Kenneth L Wright
B cell activation is dependent on a large increase in transcriptional output followed by focused expression on secreted immunoglobulin as the cell transitions to an antibody producing plasma cell. The rapid transcriptional induction is facilitated by the release of poised RNA pol II into productive elongation through assembly of the super elongation complex (SEC). We report that a SEC component, the Eleven -nineteen Lysine-rich leukemia (ELL) family member 3 (ELL3) is dynamically up-regulated in mature and activated human B cells followed by suppression as B cells transition to plasma cells in part mediated by the transcription repressor PRDM1...
August 28, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28834070/patient-navigation-for-patients-frequently-visiting-the-emergency-department-a-randomized-controlled-trial
#6
David Seaberg, Stanton Elseroad, Michael Dumas, Sudave Mendiratta, Jessica Whittle, Cheryl Hyatte, Jan Keys
BACKGROUND: ED super-utilizers (patients with 5 or more visits/year) comprise only 5% of the patients seen yet comprise 25% of total ED visits. Though the reasons for this are multifactorial, the cost to the patient and the community is exceedingly high. The cost is not just monetary; care of these patients is inappropriately fragmented and their presence in the ED may contribute to overcrowding affecting the community's emergency readiness. Previous studies using staff trained to help patients navigate their care options have had conflicting results...
August 21, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28830458/role-of-mek-partner-1-in-cancer-stemness-through-mek-erk-pathway-in-cancerous-neural-stem-cells-expressing-egfrviii
#7
Soo-Jung Kwon, Ok-Seon Kwon, Keun-Tae Kim, Young-Hyun Go, Si-In Yu, Byeong-Ha Lee, Hiroyuki Miyoshi, Eunsel Oh, Seung-Ju Cho, Hyuk-Jin Cha
BACKGROUND: Glioma stem cells (GSCs) are a major cause of the frequent relapse observed in glioma, due to their high drug resistance and their differentiation potential. Therefore, understanding the molecular mechanisms governing the 'cancer stemness' of GSCs will be particularly important for improving the prognosis of glioma patients. METHODS: We previously established cancerous neural stem cells (CNSCs) from immortalized human neural stem cells (F3 cells), using the H-Ras oncogene...
August 22, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28826927/the-integration-of-triggered-drug-delivery-with-real-time-quantification-using-fret-creating-a-super-smart-drug-delivery-system
#8
Noorjahan Aibani, Paola Fontoura da Costa, Jodie Masterson, Nino Marino, Françisco M Raymo, John Callan, Bridgeen Callan
The ability to control drug release at a specific physiological target enables the possibility of an enhanced therapeutic effect with reduced off-target toxic side effects. The discipline of controlled drug release has grown to include most areas of medicine with examples in the literature of targeted drug delivery to the majority of organs within the human body. In addition, a variety of external stimuli used to meditate the drug release process have also been investigated. Nonetheless, the concurrent real time monitoring of drug release has not been widely studied...
August 18, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28809477/reversible-reaction-based-fluorescent-probe-for-real-time-imaging-of-glutathione-dynamics-in-mitochondria
#9
Jianwei Chen, Xiqian Jiang, Chengwei Zhang, Kevin R MacKenzie, Fabio Stossi, Timothy Palzkill, Meng C Wang, Jin Wang
We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress...
August 18, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28798955/super-resolution-microscopy-reveals-a-nanoscale-organization-of-acetylcholine-receptors-for-trans-synaptic-alignment-at-neuromuscular-synapses
#10
Amanda L York, James Q Zheng
The neuromuscular junction (NMJ) is a chemical synapse formed between motoneurons and skeletal muscle fibers. The vertebrate NMJ uses acetylcholine (ACh) as the neurotransmitter and features numerous invaginations of the postsynaptic muscle membrane termed junctional folds. ACh receptors (AChRs) are believed to be concentrated on the crest of junctional folds but their spatial organization remains to be fully understood. In this study, we utilized super-resolution microscopy to examine the nanoscale organization of AChRs at NMJ...
July 2017: ENeuro
https://www.readbyqxmd.com/read/28789556/optical-determination-of-the-electronic-coupling-and-intercalation-geometry-of-thiazole-orange-homodimer-in-dna
#11
Paul D Cunningham, William P Bricker, Sebastián A Díaz, Igor L Medintz, Mark Bathe, Joseph S Melinger
Sequence-selective bis-intercalating dyes exhibit large increases in fluorescence in the presence of specific DNA sequences. This property makes this class of fluorophore of particular importance to biosensing and super-resolution imaging. Here we report ultrafast transient anisotropy measurements of resonance energy transfer (RET) between thiazole orange (TO) molecules in a complex formed between the homodimer TOTO and double-stranded (ds) DNA. Biexponential homo-RET dynamics suggest two subpopulations within the ensemble: 80% intercalated and 20% non-intercalated...
August 7, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28773174/hydrothermal-sterilization-improves-initial-osteoblast-responses-on-sandpaper-polished-titanium
#12
Xingling Shi, Lingli Xu, Qingliang Wang, Lin Xu
Hydrocarbon contamination accumulated on titanium (Ti) implant surfaces during storage and sterilization is unavoidable and difficult to remove. It impairs the bioactivity of implants, restricts initial interactions between implants and the surrounding biological environment, and has become a common challenge for Ti implants. To overcome this problem, sterilization was considered as the final surface modification and a novel method, hydrothermal sterilization (HS), was proposed. Briefly, stored sandpaper-polished Ti specimens were sterilized in a glass container with pure water at 121 °C for 20 min and kept in the same water until utilization...
July 17, 2017: Materials
https://www.readbyqxmd.com/read/28770484/high-resolution-mr-lymphangiography-for-planning-lymphaticovenous-anastomosis-treatment-a-single-centre-experience
#13
Maria Antonietta Mazzei, Francesco Gentili, Francesco Giuseppe Mazzei, Paolo Gennaro, Duccio Guerrieri, Andrea Nigri, Guido Gabriele, Elisabetta Weber, Alfonso Fausto, Giuseppe Botta, Luca Volterrani
PURPOSE: This article illustrates the feasibility of MR lymphangiography (MRL) for imaging lymphatic vessels in patients with lymphedema, its accuracy in distinguishing lymphatic vessels from veins, and its utility for planning Lymphaticovenous anastomosis (LVA) treatment. MATERIALS AND METHODS: We prospectively enrolled 30 patients (24 women, range 18-70, 17 cases of lower limb lymphedema, 6 cases of primary lymphedema). All the patients underwent MRL, using a 1...
August 2, 2017: La Radiologia Medica
https://www.readbyqxmd.com/read/28767114/in-situ-twisting-for-stabilizing-and-toughening-conductive-graphene-yarns
#14
Xi Xiang, Zhengpeng Yang, Jiangtao Di, Wujun Zhang, Ru Li, Lixing Kang, Yongyi Zhang, Haijiao Zhang, Qingwen Li
Graphene fibres are of great interest for diverse applications such as their use in supercapacitors, batteries, stimulus-responsive actuators and smart textiles. However, previously reported graphene-based fibres commonly have very low toughness and poor stretchability, which can limit many of their possible applications. We demonstrate an efficient approach for preparing super-tough yarns of reduced graphene oxides via the in situ twisting of multiplied graphene oxide fibres during fibre extrusion and coagulation, followed by chemical reduction and drying...
August 2, 2017: Nanoscale
https://www.readbyqxmd.com/read/28764379/communication-development-of-standing-evanescent-wave-fluorescence-correlation-spectroscopy-and-its-application-to-the-lateral-diffusion-of-lipids-in-a-supported-lipid-bilayer
#15
Takuhiro Otosu, Shoichi Yamaguchi
We present standing evanescent-wave fluorescence correlation spectroscopy (SEW-FCS). This technique utilizes the interference of two evanescent waves which generates a standing evanescent-wave. Fringe-pattern illumination created by a standing evanescent-wave enables us to measure the diffusion coefficients of molecules with a super-resolution corresponding to one fringe width. Because the fringe width can be reliably estimated by a simple procedure, utilization of fringes is beneficial to quantitatively analyze the slow diffusion of molecules in a supported lipid bilayer (SLB), a model biomembrane formed on a solid substrate, with the timescale relevant for reliable FCS analysis...
July 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28764343/generalized-model-for-the-diffusion-of-solvents-in-glassy-polymers-from-fickian-to-super-case-ii
#16
Jiayuan Miao, Mesfin Tsige, Philip L Taylor
The diffusion of small solvent molecules in glassy polymers may take on a variety of different forms. Fickian, anomalous, Case II and Super Case II diffusion have all been observed, and theoretical models exist that describe each specific type of behavior. Here we present a single generalized kinetic model capable of yielding all these different types of diffusion on the basis of just two parameters. The principal determinant of the type of diffusion is observed to be a dimensionless parameter, γ, that describes the influence of solvent-induced swelling in lowering the potential barriers separating available solvent sites...
July 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28757839/epidural-for-high-dose-radiation-brachytherapy-in-a-noncompliant-super-obese-patient-with-severe-pulmonary-hypertension
#17
Michael C Trawicki, Lana M Volz
High-dose radiation brachytherapy is a treatment for inoperable cervical and endometrial carcinoma. A general anesthetic is often utilized for patient comfort, facilitating patient transport to imaging resources, and allowing rapid discharge home after the procedure. Patient comorbidities, however, must be considered for every anesthetic performed. We describe a successful surgical epidural anesthetic for a patient with challenging comorbidities, including cardiac, pulmonary, and hematologic body systems.
July 2017: Saudi Journal of Anaesthesia
https://www.readbyqxmd.com/read/28752156/accounting-for-the-confound-of-meninges-in-segmenting-entorhinal-and-perirhinal-cortices-in-t1-weighted-mri
#18
Long Xie, Laura E M Wisse, Sandhitsu R Das, Hongzhi Wang, David A Wolk, Jose V Manjón, Paul A Yushkevich
Quantification of medial temporal lobe (MTL) cortices, including entorhinal cortex (ERC) and perirhinal cortex (PRC), from in vivo MRI is desirable for studying the human memory system as well as in early diagnosis and monitoring of Alzheimer's disease. However, ERC and PRC are commonly over-segmented in T1-weighted (T1w) MRI because of the adjacent meninges that have similar intensity to gray matter in T1 contrast. This introduces errors in the quantification and could potentially confound imaging studies of ERC/PRC...
October 2016: Medical Image Computing and Computer-assisted Intervention: MICCAI ..
https://www.readbyqxmd.com/read/28747311/an-n-terminal-pfs230-domain-produced-in-baculovirus-as-a-biological-active-transmission-blocking-vaccine-candidate
#19
Shwu-Maan Lee, Chia-Kuei Wu, Jordan L Plieskatt, Kazutoyo Miura, John M Hickey, C Richter King
Transmission-blocking vaccines have the potential to accelerate malaria parasite elimination by inducing antibodies that block parasite transmission from humans to mosquitoes. Pfs230, a gametocyte surface protein involved in gamete function, has long been a promising candidate. Due to the large size (3,135 amino acids), complex domains, and repeating six-cysteine (6-Cys) motifs with a multitude of disulfide bonds, the feasibility of expression of a full-length protein has been difficult. A priority focus, therefore, has been on the generation of single domains, including N-terminal fragments...
July 26, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28741935/super-photostable-phosphole-based-dye-for-multiple-acquisition-stimulated-emission-depletion-imaging
#20
Chenguang Wang, Masayasu Taki, Yoshikatsu Sato, Aiko Fukazawa, Tetsuya Higashiyama, Shigehiro Yamaguchi
As stimulated emission depletion (STED) microscopy can provide structural details of cells with an optical resolution beyond the diffraction limit, it has become an indispensable tool in cell biology. However, the intense STED laser beam usually causes rapid photobleaching of the employed fluorescent dyes, which significantly limits the utility of STED microscopy from a practical perspective. Herein we report a new design of super-photostable dye, PhoxBright 430 (PB430), comprising a fully ring-fused π-conjugated skeleton with an electron-accepting phosphole P-oxide unit...
July 25, 2017: Journal of the American Chemical Society
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