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R2r3 myb

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https://www.readbyqxmd.com/read/28523445/ptrmyb57-contributes-to-the-negative-regulation-of-anthocyanin-and-proanthocyanidin-biosynthesis-in-poplar
#1
Shuzhen Wan, Chaofeng Li, Xiaodong Ma, Keming Luo
A novel R2R3 MYB transcription factor PtrMYB57 interacted with bHLH131 and PtrTTG1 to form the MBW complex and negatively regulated the biosynthesis of both anthocyanins and PAs in poplar. R2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs involved in anthocyanin and proanthocyanidin (PA) biosynthesis have been identified in poplar. In this study, we report the identification and characterization of a subgroup 4 MYB member PtrMYB57, which contains a repressor domain (LxLxL) at the C-terminal end...
May 18, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28508121/overexpression-of-smmyb9b-enhances-tanshinone-concentration-in-salvia-miltiorrhiza-hairy-roots
#2
Jingxian Zhang, Lubin Zhou, Xiaoyu Zheng, Jinjia Zhang, Li Yang, Ronghui Tan, Shujuan Zhao
A Salvia miltiorrhiza R2R3-MYB gene, SmMYB9b , has been cloned and characterized. Overexpression of SmMYB9b resulted in a significant improvement of tanshinones, the lipophilic active ingredients in danshen hairy roots. Plant R2R3-MYB transcription factors play important roles in various physiological and biochemical processes. Danshen (Salvia miltiorrhiza bunge) is a valuable medicinal herb with tanshinones and salvianolic acids as the principal bioactive ingredients. A number of putative R2R3-MYB transcription factors have been identified in the plant, but their function remains to be studied...
May 15, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28489859/an-r2r3-type-myb-transcription-factor-gmmyb29-regulates-isoflavone-biosynthesis-in-soybean
#3
Shanshan Chu, Jiao Wang, Ying Zhu, Shulin Liu, Xiaoqiong Zhou, Huairen Zhang, Chun-E Wang, Wenming Yang, Zhixi Tian, Hao Cheng, Deyue Yu
Isoflavones comprise a group of secondary metabolites produced almost exclusively by plants in the legume family, including soybean [Glycine max (L.) Merr.]. They play vital roles in plant defense and have many beneficial effects on human health. Isoflavone content is a complex quantitative trait controlled by multiple genes, and the genetic mechanisms underlying isoflavone biosynthesis remain largely unknown. Via a genome-wide association study (GWAS), we identified 28 single nucleotide polymorphisms (SNPs) that are significantly associated with isoflavone concentrations in soybean...
May 10, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28483879/an-r2r3-myb-transcription-factor-in-capsaicinoid-biosynthesis
#4
Magda Lisette Arce-Rodríguez, Neftalí Ochoa-Alejo
Capsaicinoids are responsible for the hot taste of chili peppers. They are restricted to the genus Capsicum and are synthesized by the acylation of the aromatic compound vanillylamine (derived from the phenylpropanoid pathway) with a branched-chain fatty acid by the catalysis of the putative enzyme capsaicinoid synthase. R2R3-MYB transcription factors have been reported in different species of plants as regulators of structural genes of the phenylpropanoid pathway; therefore, we hypothesized that MYB genes might be involved in the regulation of the biosynthesis of pungent compounds...
May 8, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28483877/lnk1-and-lnk2-co-repressors-interact-with-the-myb3-transcription-factor-in-phenylpropanoid-biosynthesis
#5
Meiliang Zhou, Kaixuan Zhang, Zhanmin Sun, Mingli Yan, Cheng Chen, Xinquan Zhang, Yixiong Tang, Yanmin Wu
Subgroup 4 of R2R3-MYB transcription factors (TFs) consist of four members, MYB3, MYB4, MYB7 and MYB32, which possess the conserved EAR repression motif (pdLHLD/LLxiG/S) in their C-termini. Here, we show that MYB3 is a newly identified repressor in Arabidopsis phenylpropanoids biosynthesis. However, the repression mechanism of MYB3 is completely different from MYB4, MYB7 and MYB32. Yeast two hybrid screening using MYB3 as a bait isolates NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED1 (LNK1) and LNK2, members of a small family of four LNK proteins...
May 8, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28460279/overexpression-of-a-tartary-buckwheat-r2r3-myb-transcription-factor-gene-ftmyb9-enhances-tolerance-to-drought-and-salt-stresses-in-transgenic-arabidopsis
#6
Fei Gao, Jing Zhou, Ren-Yu Deng, Hai-Xia Zhao, Cheng-Lei Li, Hui Chen, Tatsuro Suzuki, Sang-Un Park, Qi Wu
Tartary buckwheat (Fagopyrum tataricum) is a traditional coarse cereal that exhibits strong plasticity in its adaptation to harsh and complicated environmental stresses. In an attempt to study the strong tolerance of tartary buckwheat, the FtMYB9 gene, which encodes an R2R3-MYB transcription factor protein, was functionally investigated. FtMYB9 expression was rapidly and strongly induced by ABA, cold, salt, and drought treatments in the seedling stage. A yeast one-hybrid system assay indicated that FtMYB9 is an activator of transcriptional activity, consistent with its roles as a transcription factor...
April 13, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28452060/cimyb17-a-stress-induced-chicory-r2r3-myb-transcription-factor-activates-promoters-of-genes-involved-in-fructan-synthesis-and-degradation
#7
Hongbin Wei, Anja Bausewein, Steffen Greiner, Nicolas Dauchot, Karsten Harms, Thomas Rausch
In Cichorium intybus, inulin metabolism is mediated by fructan-active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), and fructan 1-exohydrolases 1, 2a and 2b (1-FEH1, -2a and -2b), respectively. While these enzymes have been rigorously characterized, the transcriptional network orchestrating their development- and stress-related expression has remained largely unknown. Here, the possible role of R2R3-MYB transcription factors in FAZY regulation was explored via bioinformatic identification of R2R3-MYBs (using an RNA sequencing (RNAseq) database), studies of co-expression of these factors with target genes, in vivo transient transactivation assays of FAZY target promoters (dual luciferase assay), and a yeast one-hybrid assay investigating the specificity of the binding of these factors to cis-elements...
April 28, 2017: New Phytologist
https://www.readbyqxmd.com/read/28429079/the-r2r3-myb-tt2b-and-the-bhlh-tt8-genes-are-the-major-regulators-of-proanthocyanidin-biosynthesis-in-the-leaves-of-lotus-species
#8
Francisco José Escaray, Valentina Passeri, Ana Perea-García, Cristian Javier Antonelli, Francesco Damiani, Oscar Adolfo Ruiz, Francesco Paolocci
By exploiting interspecific hybrids and their progeny, we identified key regulatory and transporter genes intimately related to proanthocyanidin biosynthesis in leaves of Lotus spp. Proanthocyanidins (PAs), known as condensed tannins, are polymeric flavonoids enriching forage legumes of key nutritional value to prevent bloating in ruminant animals. Unfortunately, major forage legumes such as alfalfa and clovers lack PAs in edible tissues. Therefore, engineering the PA trait in herbage of forage legumes is paramount to improve both ecological and economical sustainability of cattle production system...
April 20, 2017: Planta
https://www.readbyqxmd.com/read/28423209/r2r3-myb-transcription-factor-mdmyb73-is-involved-in-malate-accumulation-and-vacuolar-acidification-in-apple
#9
Da-Gang Hu, Yuan-Yuan Li, Quan-Yan Zhang, Ming Li, Cui-Hui Sun, Jian-Qiang Yu, Yu-Jin Hao
Malate, the predominant organic acid in many fruits, is a crucial component of fruit organoleptic quality, including taste and flavor. The genetic and environmental mechanisms affecting malate metabolism in fruit cells have been studied extensively. However, the transcriptional regulation of malate-metabolizing enzymes and vacuolar transporters remains poorly understood. Our previous studies demonstrated that MdMYB1 modulates anthocyanin accumulation and vacuolar acidification, by directly activating vacuolar transporters, including MdVHA-B1, MdVHA-E, MdVHP1, and MdtDT...
April 19, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28421329/characterization-of-two-tt2-type-myb-transcription-factors-regulating-proanthocyanidin-biosynthesis-in-tetraploid-cotton-gossypium-hirsutum
#10
Nan Lu, Marissa Roldan, Richard A Dixon
Two TT2-type MYB transcription factors identified from tetraploid cotton are involved in regulating proanthocyanidin biosynthesis, providing new strategies for engineering condensed tannins in crops. Proanthocyanidins (PAs), also known as condensed tannins, are important secondary metabolites involved in stress resistance in plants, and are health supplements that help to reduce cholesterol levels. As one of the most widely grown crops in the world, cotton provides the majority of natural fabrics and is a supplemental food for ruminant animals...
April 18, 2017: Planta
https://www.readbyqxmd.com/read/28386931/subfunctionalization-of-duplicate-myb-genes-in-solanum-commersonii-generated-the-cold-induced-scan2-and-the-anthocyanin-regulator-scan1
#11
Vincenzo D'Amelia, Riccardo Aversano, Alessandra Ruggiero, Giorgia Batelli, Ingo Appelhagen, Claudio Dinacci, Lionel Hill, Cathie Martin, Domenico Carputo
Wild potato species are useful sources allelic diversity and loci lacking in the cultivated potato. In these species, the presence of anthocyanins in leaves has been associated with a greater tolerance to cold stress. However, the molecular mechanisms that allow potatoes to withstand cold exposure remain unclear. Here, we show that the expression of AN2, a MYB transcription factor, is induced by low temperatures in wild, cold tolerant Solanum commersonii, and not in susceptible S. tuberosum varieties. We found that AN2 is a paralog of the potato anthocyanin regulator AN1, showing similar interaction ability with bHLH co-partners...
April 7, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28370629/a-group-of-grapevine-myba-transcription-factors-located-in-chromosome-14-control-anthocyanin-synthesis-in-vegetative-organs-with-different-specificities-compared-to-the-berry-color-locus
#12
José Tomás Matus, Erika Cavallini, Rodrigo Loyola, Janine Höll, Laura Finezzo, Silvia Dal Santo, Sandrine Vialet, Mauro Commisso, Federica Roman, Andrea Schubert, José Antonio Alcalde, Jochen Bogs, Agnès Ageorges, Giovanni Battista Tornielli, Patricio Arce-Johnson
Grapevine organs accumulate anthocyanins in a cultivar-specific and environmentally induced manner. The MYBA1-A2 genes within the berry color locus in chromosome 2 represent the major genetic determinants of fruit color. The simultaneous occurrence of transposon insertions and point mutations in these genes is responsible for most white-skinned phenotypes. However, red pigmentation found in vegetative organs suggests the presence of additional regulators. This work describes a genomic region of chromosome 14 containing three closely related R2R3-MYB genes, named MYBA5, MYBA6 and MYBA7...
April 2, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28361257/induction-of-anthocyanin-in-the-inner-epidermis-of-red-onion-leaves-by-environmental-stimuli-and-transient-expression-of-transcription-factors
#13
Elizabeth J Wiltshire, Colin C Eady, David A Collings
Novel imaging approaches have allowed measurements of the anthocyanin induction in onion epidermal cells that can be induced through water stress or transient expression of exogenous transcription factors. Environmental and genetic mechanisms that allow the normally colourless inner epidermal cells of red onion (Allium cepa) bulbs to accumulate anthocyanin were quantified by both absorbance ratios and fluorescence. We observed that water-stressing excised leaf segments induced anthocyanin formation, and fluorescence indicated that this anthocyanin was spectrally similar to the anthocyanin in the outer epidermal cells...
March 30, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28348066/poplar-myb115-and-myb134-transcription-factors-regulate-proanthocyanidin-synthesis-and-structure
#14
Amy Midori James, Dawei Ma, Robin Mellway, Andreas Gesell, Kazuko Yoshida, Vincent Walker, Lan Tran, Don Stewart, Michael Reichelt, Jussi Suvanto, Juha-Pekka Salminen, Jonathan Gershenzon, Armand Séguin, C Peter Constabel
The accumulation of proanthocyanidins is regulated by a complex of transcription factors composed of R2R3 MYB, basic helix-loop-helix, and WD40 proteins that activate the promoters of biosynthetic genes. In poplar (genus Populus), MYB134 is known to regulate proanthocyanidin biosynthesis by activating key flavonoid genes. Here, we characterize a second MYB regulator of proanthocyanidins, MYB115. Transgenic poplar overexpressing MYB115 showed a high-proanthocyanidin phenotype and reduced salicinoid accumulation, similar to the effects of MYB134 overexpression...
May 2017: Plant Physiology
https://www.readbyqxmd.com/read/28345675/a-myb-transcription-factor-dcmyb6-is-involved-in-regulating-anthocyanin-biosynthesis-in-purple-carrot-taproots
#15
Zhi-Sheng Xu, Kai Feng, Feng Que, Feng Wang, Ai-Sheng Xiong
Carrots are widely grown and enjoyed around the world. Purple carrots accumulate rich anthocyanins in the taproots, while orange, yellow, and red carrots accumulate rich carotenoids in the taproots. Our previous studies indicated that variation in the activity of regulatory genes may be responsible for variations in anthocyanin production among various carrot cultivars. In this study, an R2R3-type MYB gene, designated as DcMYB6, was isolated from a purple carrot cultivar. In a phylogenetic analysis, DcMYB6 was grouped into an anthocyanin biosynthesis-related MYB clade...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287502/cmmyb19-over-expression-improves-aphid-tolerance-in-chrysanthemum-by-promoting-lignin-synthesis
#16
Yinjie Wang, Liping Sheng, Huanru Zhang, Xinping Du, Cong An, Xiaolong Xia, Fadi Chen, Jiafu Jiang, Sumei Chen
The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the localized to the localized to the localized to the localized to the localized to the nucleus nucleus . CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root...
March 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28285084/a-salt-stress-regulator-from-the-poplar-r2r3-myb-family-integrates-the-regulation-of-lateral-root-emergence-and-aba-signaling-to-mediate-salt-stress-tolerance-in-arabidopsis
#17
Qing Fang, Tianzhi Jiang, Liangxiang Xu, Hai Liu, Hui Mao, Xianqiang Wang, Bo Jiao, Yanjiao Duan, Qiong Wang, Qiannan Dong, Li Yang, Guozheng Tian, Chi Zhang, Yifeng Zhou, Xiaopeng Liu, Haiyang Wang, Di Fan, Bangjun Wang, Keming Luo
The roles of most MYB transcription factors (TFs) in the poplar remain unclear. Here, we demonstrate that PtrSSR1, a salt-stress-regulator in the Populus trichocarpa R2R3 MYB gene family, mediates the tolerance of transgenic Arabidopsis plants to salt stress. The transcripts of PtrSSR1 could be induced by salt stress rapidly in poplar. Subcellular localization and yeast assays indicated that PtrSSR1 encoded a nuclear protein with transactivation activity. The Arabidopsis transformants overexpressing PtrSSR1 clearly displayed lateral root emergence (LRE) inhibition compared with wild-type (Wt) under normal conditions; while upon NaCl treatment, the transformants showed improved tolerance, and the LRs emerged faster from salt-induced inhibition...
February 24, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28284041/the-atmyb12-activation-domain-maps-to-a-short-c-terminal-region-of-the-transcription-factor
#18
Ralf Stracke, Neslihan Turgut-Kara, Bernd Weisshaar
The Arabidopsis thaliana R2R3-MYB transcription factor MYB12 is a light-inducible, flavonol-specific activator of flavonoid biosynthesis. The transactivation activity of the AtMYB12 protein was analyzed using a C-terminal deletion series in a transient A. thaliana protoplast assay with the goal of mapping the activation domain (AD). Although the deletion of the last 46 C-terminal amino acids did not affect the activation capacity, the deletion of the last 98 amino acids almost totally abolished transactivation of two different target promoters...
March 11, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28267260/comparative-transcriptomic-analysis-of-key-genes-involved-in-flavonoid-biosynthetic-pathway-and-identification-of-a-flavonol-synthase-from-artemisia-annua-l
#19
Shuoqian Liu, Liping Liu, Yuwei Tang, Shuo Xiong, Jinhua Long, Zhonghua Liu, Na Tian
(1) The regulation mechanism of flavonoids, which were found to synergize anti-malarial and anti-cancer compounds in Artemisia annua, is still unclear. (2) In this study, an anthocyanidin-accumulating mutant callus was induced from A. annua, and then comparative transcriptomic analysis of the wild-type and mutant calli was performed, based on the next-generation Illumina/Solexa sequencing platform and de novo assembly. (3) As a result, a total of 82,393 unigenes were obtained and 34,764 unigenes were annotated in the public database...
March 7, 2017: Plant Biology
https://www.readbyqxmd.com/read/28255171/mcmyb12-transcription-factors-co-regulate-proanthocyanidin-and-anthocyanin-biosynthesis-in-malus-crabapple
#20
Ji Tian, Jie Zhang, Zhen-Yun Han, Ting-Ting Song, Jin-Yan Li, Ya-Ru Wang, Yun-Cong Yao
The flavonoid compounds, proanthocyanidins (PAs), protect plants from biotic stresses, contribute to the taste of many fruits, and are beneficial to human health in the form of dietary antioxidants. In this study, we functionally characterized two Malus crabapple R2R3-MYB transcription factors, McMYB12a and McMYB12b, which co-regulate PAs and anthocyanin biosynthesis. McMYB12a was shown to be mainly responsible for upregulating the expression of anthocyanin biosynthetic genes by binding to their promoters, but to be only partially responsible for regulating PAs biosynthetic genes...
March 3, 2017: Scientific Reports
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