Read by QxMD icon Read

R2r3 myb

Marçal Soler, Anna Plasencia, Jorge Lepikson-Neto, Eduardo L O Camargo, Annabelle Dupas, Nathalie Ladouce, Edouard Pesquet, Fabien Mounet, Romain Larbat, Jacqueline Grima-Pettenati
Comparative phylogenetic analyses of the R2R3-MYB transcription factor family revealed that five subgroups were preferentially found in woody species and were totally absent from Brassicaceae and monocots (Soler et al., 2015). Here, we analyzed one of these subgroups (WPS-I) for which no gene had been yet characterized. Most Eucalyptus members of WPS-I are preferentially expressed in the vascular cambium, the secondary meristem responsible for tree radial growth. We focused on EgMYB88, which is the most specifically and highly expressed in vascular tissues, and showed that it behaves as a transcriptional activator in yeast...
2016: Frontiers in Plant Science
M He, H Wang, Y Z Liu, W J Gao, Y H Gao, F Wang, Y W Zhou
v-myb avianmyeloblastosis viral oncogene homolog (MYB) transcription factors are key regulators of stress responsive gene expression in plants. In this study, the MYB gene, ChiMYB (GenBank accession No. KT948997), was isolated from Chrysanthemum indicum, and was functionally characterized with an emphasis on salinity stress tolerance. The full ChiMYB cDNA sequence (948 bp) encoded a typical R2R3 MYB transcription factor that contained 315 amino acid residues and two MYB domains. The temporal expression pattern of ChiMYB was noted in C...
September 23, 2016: Genetics and Molecular Research: GMR
X B Luo, Z Liu, L Xu, Y Wang, X W Zhu, W Zhang, W Chen, Y L Zhu, X J Su, M Everlyne, L W Liu
Glucosinolates (GSLs) are important secondary metabolites in Brassicaceae plants. Previous studies have mainly focused on GSL contents, types, and biosynthesis-related genes, but the molecular characterization patterns of GSL biosynthesis-related transcription factors remain largely unexplored in radish (Raphanus sativus L.). To isolate transcription factor genes regulating the GSL biosynthesis, genomic DNA and cDNA sequences of RsMYB28 and RsMYB29 genes were isolated in radish. Two R2R3-MYB domains were identified in the deduced amino acid sequences...
September 23, 2016: Genetics and Molecular Research: GMR
Jing Zhang, Hang Ge, Chen Zang, Xian Li, Donald Grierson, Kun-Song Chen, Xue-Ren Yin
Lignin is important for plant secondary cell wall formation and participates in resistance to various biotic and abiotic stresses. Loquat undergoes lignification not only in vegetative tissues but also in flesh of postharvest fruit, which adversely affects consumer acceptance. Thus, researches on lignin biosynthesis and regulation are important to understand loquat fruit lignification. In loquat, a gene encoding an enzyme in the lignin biosynthesis pathway, Ej4CL1, was reported to be regulated by transcription factors, including EjMYB1, EjMYB2, EjMYB8, and EjAP2-1, knowledge of this process is still limited...
2016: Frontiers in Plant Science
Shan-Shan Sun, Paul F Gugger, Qing-Feng Wang, Jin-Ming Chen
The lotus (Nelumbonaceae: Nelumbo Adans.) is a highly desired ornamental plant, comprising only two extant species, the sacred lotus (N. nucifera Gaerten.) with red flowers and the American lotus (N. lutea Willd.) with yellow flowers. Flower color is the most obvious difference of two species. To better understand the mechanism of flower color differentiation, the content of anthocyanins and the expression levels of four key structural genes (e.g., DFR, ANS, UFGT and GST) were analyzed in two species. Our results revealed that anthocyanins were detected in red flowers, not yellow flowers...
2016: PeerJ
José Tomás Matus
Plants are constantly challenged by environmental fluctuations. In response, they have developed a wide range of morphological and biochemical adaptations committed to ameliorate the effects of abiotic stress. When exposed to higher solar radiation levels, plants activate the synthesis of a large set of enzymes and secondary metabolites as part of a complex sunscreen and antioxidant defense mechanism. Grapevine (Vitis vinifera L.) has become a widely used system for studying adaptive responses to this type of stress since changes in berry composition, positively influenced by increased ultraviolet (UV) radiation levels, improve the quality of wines subsequently produced...
2016: Frontiers in Plant Science
Lulu Xie, Fei Li, Shifan Zhang, Hui Zhang, Wei Qian, Peirong Li, Shujiang Zhang, Rifei Sun
Introgression breeding is a widely used method for the genetic improvement of crop plants; however, the mechanism underlying candidate gene flow patterns during hybridization is poorly understood. In this study, we used a powerful pipeline to investigate a Chinese cabbage (Brassica rapa L. ssp. pekinensis) introgression line with the anthocyanin overaccumulation phenotype. Our purpose was to analyze the gene flow patterns during hybridization and elucidate the genetic factors responsible for the accumulation of this important pigment compound...
2016: Frontiers in Plant Science
Binmei Sun, Zhangsheng Zhu, Panrong Cao, Hao Chen, Changming Chen, Xin Zhou, Yanhui Mao, Jianjun Lei, Yanpin Jiang, Wei Meng, Yingxi Wang, Shaoqun Liu
Purple foliage always appears in Camellia sinensis families; however, the transcriptional regulation of anthocyanin biosynthesis is unknown. The tea bud sport cultivar 'Zijuan' confers an abnormal pattern of anthocyanin accumulation, resulting in a mutant phenotype that has a striking purple color in young foliage and in the stem. In this study, we aimed to unravel the underlying molecular mechanism of anthocyanin biosynthetic regulation in C. sinensis. Our results revealed that activation of the R2R3-MYB transcription factor (TF) anthocyanin1 (CsAN1) specifically upregulated the bHLH TF CsGL3 and anthocyanin late biosynthetic genes (LBGs) to confer ectopic accumulation of pigment in purple tea...
2016: Scientific Reports
Zhenqian Zhang, Xiaona Hu, Yunqin Zhang, Zhenyan Miao, Can Xie, Xiangzhao Meng, Jie Deng, Jiangqi Wen, Kirankumar S Mysore, Florian Frugier, Tao Wang, Jiangli Dong
Cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. C-REPEAT BINDING FACTOR1 (CBF1), CBF2, and CBF3 genes were shown previously to participate in cold acclimation in Medicago truncatula In addition, MtCBF4 is transcriptionally induced by salt, drought, and cold stresses. We show here that MtCBF4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. To identify molecular factors acting upstream and downstream of the MtCBF4 transcription factor (TF) in cold responses, we first identified genes that are differentially regulated upon MtCBF4 overexpression using RNAseq Digital Gene Expression Profiling...
October 2016: Plant Physiology
Wenbin Liao, Yiling Yang, Yayun Li, Gan Wang, Ming Peng
Cassava plants (Manihot esculenta Crantz) resist environmental stresses by shedding leaves in leaf pulvinus abscission zones (AZs), thus leading to adaptation to new environmental conditions. Little is known about the roles of cassava R2R3 MYB factors in regulating AZ separation. Herein, 166 cassava R2R3 MYB genes were identified. Evolutionary analysis indicated that the 166 R2R3 MYB genes could be divided into 11 subfamilies. Transcriptome analysis indicated that 26 R2R3 MYB genes were expressed in AZs across six time points during both ethylene- and water-deficit stress-induced leaf abscission...
2016: Scientific Reports
Luming Yao, Yina Jiang, Xinxin Lu, Biao Wang, Pei Zhou, Tianlong Wu
Few regulators for drought tolerance have been identified in Lablab purpureus which is a multipurpose leguminous crop. The transcription factor MYB is involved in regulatory networks in response to abiotic and biotic stresses in plants. A novel R2R3-MYB factor in L. purpureus has been identified. An suppression subtraction hybridization (SSH) library was constructed using root tissues of L. purpureus MEIDOU 2012 from well-watered and water-stress treatments that were subjected to drought stress for 10 days...
October 2016: Molecular Biology Reports
Meng-Jun Li, Yu Qiao, Ya-Qing Li, Zhan-Liang Shi, Nan Zhang, Cai-Li Bi, Jin-Kao Guo
We isolated the TaMYBsm1 genes, encoding R2R3-type MYB proteins in common wheat, aimed to uncover the possible molecular mechanisms related to drought response. The TaMYBsm1 genes, TaMYBsm1-A, TaMYBsm1-B and TaMYBsm1-D, were isolated and analyzed from the common wheat cultivar Shimai 15. Their expression patterns under PEG 6000 and mannitol were monitored by semi-quantitative RT-PCR and β-glucuronidase (Gus) assay. The function of TaMYBsm1-D under drought stress in transgenic Arabidopsis plants was investigated, and the germination rate, water loss rate, as well as the proline and malondialdehyde (MDA) content were compared with that in wild type (WT) plants...
August 19, 2016: Journal of Plant Research
Wenjun Huang, A B M Khaldun, Jianjun Chen, Chanjuan Zhang, Haiyan Lv, Ling Yuan, Ying Wang
Flavonols as plant secondary metabolites with vital roles in plant development and defense against UV light, have been demonstrated to be the main bioactive components (BCs) in the genus Epimedium plants, several species of which are used as materials for Herba Epimedii, an important traditional Chinese medicine. The flavonol biosynthetic pathway genes had been already isolated from Epimedium sagittatum, but a R2R3-MYB transcription factor regulating the flavonol synthesis has not been functionally characterized so far in Epimedium plants...
2016: Frontiers in Plant Science
Md Kamrul Hasan, Congcong Liu, Fanan Wang, Golam Jalal Ahammed, Jie Zhou, Ming-Xing Xu, Jing-Quan Yu, Xiao-Jian Xia
Glutathione (GSH) plays a critical role in plant growth, development and responses to stress. However, the mechanism by which GSH regulates tolerance to cadmium (Cd) stress still remains unclear. Here we show that inhibition of GSH biosynthesis by buthionine sulfoximine (BSO) aggravated Cd toxicity by increasing accumulation of reactive oxygen species (ROS) and reducing contents of nitric oxide (NO) and S-nitrosothiol (SNO) in tomato roots. In contrast, exogenous GSH alleviated Cd toxicity by substantially minimizing ROS accumulation and increasing contents of NO and SNO, and activities of antioxidant enzymes that eventually reduced oxidative stress...
October 2016: Chemosphere
Yueqing Li, Xiaotong Shan, Ruifang Gao, Song Yang, Shucai Wang, Xiang Gao, Li Wang
The MBW complex, comprised by R2R3-MYB, basic helix-loop-helix (bHLH) and WD40, is a single regulatory protein complex that drives the evolution of multiple traits such as flavonoid biosynthesis and epidermal cell differentiation in plants. In this study, two IIIf Clade-bHLH regulator genes, FhGL3L and FhTT8L, were isolated and functionally characterized from Freesia hybrida. Different spatio-temporal transcription patterns were observed showing diverse correlation with anthocyanin and proanthocyanidin accumulation...
2016: Scientific Reports
Chonghui Li, Jian Qiu, Guangsui Yang, Surong Huang, Junmei Yin
A R2R3-MYB gene AaMYB2 was isolated from Anthurium andraeanum (Hort.) and was functionally characterized to be a positive transcriptional regulator for anthocyanin biosynthesis in the spathes and leaves. Spathe coloration is an important Anthurium andraeanum (Hort.) characteristic, which is mainly contributed by anthocyanins. R2R3-MYB transcription factors (TFs) are important regulators of anthocyanin biosynthesis in plants. Here we describe the identification and characterization of AaMYB2, a member in subgroup 6 of the R2R3-MYB TFs family, which correlated with anthocyanin biosynthesis in A...
October 2016: Plant Cell Reports
Haiyan Hu, Xin He, Lili Tu, Longfu Zhu, Sitao Zhu, Zonghe Ge, Xianlong Zhang
Jasmonic acid (JA) signalling has been well studied in Arabidopsis. Most reports focus on the role of JA in biological pathways such as stress resistance, trichome initiation and anthocyanin accumulation. The JASMONATE ZIM-DOMAIN (JAZ) protein is one of the important repressors in the JA signalling pathway. Previous studies showed that JA functions in fiber initiation and elongation, but little is known about how JAZ genes function in fiber development. In this study, a cotton JAZ protein (GhJAZ2) containing a highly conserved TIFY motif and a C-terminal Jas domain was identified, and its function during cotton fiber development was analysed...
July 15, 2016: Plant Journal: for Cell and Molecular Biology
Darren Chern Jan Wong, Rudolf Schlechter, Alessandro Vannozzi, Janine Höll, Ibrahim Hmmam, Jochen Bogs, Giovanni Battista Tornielli, Simone Diego Castellarin, José Tomás Matus
R2R3-MYB transcription factors (TFs) belong to a large and functionally diverse protein superfamily in plants. In this study, we explore the evolution and function of this family in grapevine (Vitis vinifera L.), a high-value fruit crop. We identified and manually curated 134 genes using RNA-Seq data, and named them systematically according to the Super-Nomenclature Committee. We identified novel genes, splicing variants and grapevine/woody-specific duplicated subgroups, suggesting possible neo- and sub-functionalization events...
July 12, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Margaret F Hendrick, Findley R Finseth, Minna E Mathiasson, Kristen A Palmer, Emma M Broder, Peter Breigenzer, Lila Fishman
Microgeographic adaptation provides a particularly interesting context for understanding the genetic basis of phenotypic divergence, and may also present unique empirical challenges. In particular, plant adaptation to extreme soil mosaics may generate barriers to gene flow or shifts in mating system that confound simple genomic scans for adaptive loci. Here, we combine three approaches - QTL mapping of candidate intervals in controlled crosses, population resequencing (PoolSeq), and analyses of wild recombinant individuals - to investigate one trait associated with Mimulus guttatus (yellow monkeyflower) adaptation to geothermal soils in Yellowstone National Park...
July 9, 2016: Molecular Ecology
Daniel J Gates, Susan R Strickler, Lukas A Mueller, Bradley J S C Olson, Stacey D Smith
MYB transcription factors play an important role in regulating key plant developmental processes involving defense, cell shape, pigmentation, and root formation. Within this gene family, sequences containing an R2R3 MYB domain are the most abundant type and exhibit a wide diversity of functions. In this study, we identify 559 R2R3 MYB genes using whole genome data from four species of Solanaceae and reconstruct their evolutionary relationships. We compare the Solanaceae R2R3 MYBs to the well-characterized Arabidopsis thaliana sequences to estimate functional diversity and to identify gains and losses of MYB clades in the Solanaceae...
August 2016: Journal of Molecular Evolution
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"