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https://www.readbyqxmd.com/read/28651344/uterine-responses-to-early-pre-attachment-embryos-in-the-domestic-dog-and-comparisons-with-other-domestic-animal-species
#1
Felix R Graubner, Aykut Gram, Ewa Kautz, Stefan Bauersachs, Selim Aslan, Ali R Agaoglu, Alois Boos, Mariusz P Kowalewski
In the dog there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal...
June 23, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28651174/label-free-chronopotentiometric-glycoprofiling-of-prostate-specific-antigen-using-sialic-acid-recognizing-lectins
#2
Stefan Belicky, Hana Černocká, Tomas Bertok, Alena Holazova, Kamila Réblová, Emil Paleček, Jan Tkac, Veronika Ostatná
In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA)...
June 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28651161/connexin-43-mediates-changes-in-protein-phosphorylation-in-hk-2-cells-during-chronic-cadmium-exposure
#3
Zehe Ge, Haipeng Diao, Maohui Yu, Xiaoli Ji, Qingping Liu, Xiuli Chang, Qing Wu
Connexin 43 (Cx43) is believed to play a role in the mechanisms of toxicity of many chemical species, include cadmium (Cd). In this study, human renal proximal tubule (HK-2) cells were exposed to Cd (1μM, 10 days). Of the 584 protein residues detected using a Phospho Explorer antibody microarray (PEX100), more than half changed their levels of phosphorylation after chronic Cd exposure. Cx43 siRNA attenuated Cd-induced apoptosis and inhibited proliferation, while also attenuating changes in the levels of phosphorylation of many protein residues...
June 20, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28651148/aldo-keto-reductase-activity-after-diethylhexyl-phthalate-exposure-in-eutopic-and-ectopic-endometrial-cells
#4
La Yeon Kim, Mee Ran Kim, Jang Heub Kim, Hyun Hee Cho
OBJECTIVE: Endometriosis is a multifactorial gynaecological disease in reproductive-age women. Endometriotic tissue is characterized by high prostaglandin levels and progesterone resistance. Human aldo-keto reductases (AKRs) convert progesterone to a less potent metabolite and cause progesterone resistance. Therefore, in this study, we evaluated whether diethylhexyl phthalate (DEHP) alters AKR expression in human ectopic and eutopic endometrium. STUDY DESIGN: We used microarrays and western blotting to study the effects of DEHP, and checked the presence of AKR in endometriosis patients by enzyme-linked immunosorbent assay (ELISA)...
May 22, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28651046/a-heparan-sulfate-microarray-reveals-that-heparan-sulfate-protein-binding-exhibit-different-ligand-requirements
#5
Chengli Zong, Andre Venot, Xiuru Li, Weigang Lu, Wenyuan Xiao, Josetti Lynn Wilkes, Catherina L Salanga, Tracy M Handel, Lianchun Wang, Margreet A Wolfert, Geert-Jan Boons
Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes. Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties. The ligand requirements of HS-binding proteins have, however, been defined in only a few cases. We describe here a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides. It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation...
June 26, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28651018/meta-analysis-of-mirna-expression-profiles-for-prostate-cancer-recurrence-following-radical-prostatectomy
#6
Elnaz Pashaei, Elham Pashaei, Maryam Ahmady, Mustafa Ozen, Nizamettin Aydin
BACKGROUND: Prostate cancer (PCa) is a leading reason of death in men and the most diagnosed malignancies in the western countries at the present time. After radical prostatectomy (RP), nearly 30% of men develop clinical recurrence with high serum prostate-specific antigen levels. An important challenge in PCa research is to identify effective predictors of tumor recurrence. The molecular alterations in microRNAs are associated with PCa initiation and progression. Several miRNA microarray studies have been conducted in recurrence PCa, but the results vary among different studies...
2017: PloS One
https://www.readbyqxmd.com/read/28651004/a-human-endogenous-retrovirus-derived-gene-that-can-contribute-to-oncogenesis-by-activating-the-erk-pathway-and-inducing-migration-and-invasion
#7
Cécile Lemaître, Jhen Tsang, Caroline Bireau, Thierry Heidmann, Marie Dewannieux
Endogenous retroviruses are cellular genes of retroviral origin captured by their host during the course of evolution and represent around 8% of the human genome. Although most are defective and transcriptionally silenced, some are still able to generate retroviral-like particles and proteins. Among these, the HERV-K(HML2) family is remarkable since its members have amplified relatively recently and many of them still have full length coding genes. Furthermore, they are induced in cancers, especially in melanoma, breast cancer and germ cell tumours, where viral particles, as well as the envelope protein (Env), can be detected...
June 26, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28651000/assessing-the-impact-of-benzo-a-pyrene-on-marine-mussels-application-of-a-novel-targeted-low-density-microarray-complementing-classical-biomarker-responses
#8
Mohamed Banni, Susanna Sforzini, Volker M Arlt, Audrey Barranger, Lorna J Dallas, Caterina Oliveri, Yann Aminot, Beniamina Pacchioni, Caterina Millino, Gerolamo Lanfranchi, James W Readman, Michael N Moore, Aldo Viarengo, Awadhesh N Jha
Despite the increasing use of mussels in environmental monitoring and ecotoxicological studies, their genomes and gene functions have not been thoroughly explored. Several cDNA microarrays were recently proposed for Mytilus spp., but putatively identified partial transcripts have rendered the generation of robust transcriptional responses difficult in terms of pathway identification. We developed a new low density oligonucleotide microarray with 465 probes covering the same number of genes. Target genes were selected to cover most of the well-known biological processes in the stress response documented over the last decade in bivalve species at the cellular and tissue levels...
2017: PloS One
https://www.readbyqxmd.com/read/28650989/tumor-tissue-protein-signatures-reflect-histological-grade-of-breast-cancer
#9
Petter Skoog, Mattias Ohlsson, Mårten Fernö, Lisa Rydén, Carl A K Borrebaeck, Christer Wingren
Histological grade is one of the most commonly used prognostic factors for patients diagnosed with breast cancer. However, conventional grading has proven technically challenging, and up to 60% of the tumors are classified as histological grade 2, which represents a heterogeneous cohort less informative for clinical decision making. In an attempt to study and extend the molecular puzzle of histologically graded breast cancer, we have in this pilot project searched for additional protein biomarkers in a new space of the proteome...
2017: PloS One
https://www.readbyqxmd.com/read/28650445/a-distinct-function-of-the-retinoblastoma-protein-in-the-control-of-lipid-composition-identified-by-lipidomic-profiling
#10
H Muranaka, A Hayashi, K Minami, S Kitajima, S Kohno, Y Nishimoto, N Nagatani, M Suzuki, L A N Kulathunga, N Sasaki, N Okada, T Matsuzaka, H Shimano, H Tada, C Takahashi
Here, by combining lipidomics with transcriptome analysis, we demonstrate that Rb depletion in mouse embryonic fibroblastss induces significant alterations in their lipid composition. We discovered that Rb depletion induced increase in lysophosphatidylserine, diacylglycerol (DAG), fatty acid (FA), acylcarnitine, phosphatidylcholine (PC), arachidonoyl ethanolamine, and decrease in phosphatidylglycerol, monoacylglycerol, without change in total lipid per protein levels. Analysis of the acyl chain composition of DAG, PC and phosphatidylserine revealed increase of saturated and mono-unsaturated acyl chains with specific carbon chain length...
June 26, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28649435/improved-prediction-of-parp-inhibitor-response-and-identification-of-synergizing-agents-through-use-of-a-novel-gene-expression-signature-generation-algorithm
#11
Daniel J McGrail, Curtis Chun-Jen Lin, Jeannine Garnett, Qingxin Liu, Wei Mo, Hui Dai, Yiling Lu, Qinghua Yu, Zhenlin Ju, Jun Yin, Christopher P Vellano, Bryan Hennessy, Gordon B Mills, Shiaw-Yih Lin
Despite rapid advancement in generation of large-scale microarray gene expression datasets, robust multigene expression signatures that are capable of guiding the use of specific therapies have not been routinely implemented into clinical care. We have developed an iterative resampling analysis to predict sensitivity algorithm to generate gene expression sensitivity profiles that predict patient responses to specific therapies. The resultant signatures have a robust capacity to accurately predict drug sensitivity as well as the identification of synergistic combinations...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28649419/identification-of-transcription-factors-that-promote-the-differentiation-of-human-pluripotent-stem-cells-into-lacrimal-gland-epithelium-like-cells
#12
Masatoshi Hirayama, Shigeru B H Ko, Tetsuya Kawakita, Tomohiko Akiyama, Sravan K Goparaju, Atsumi Soma, Yuhki Nakatake, Miki Sakota, Nana Chikazawa-Nohtomi, Shigeto Shimmura, Kazuo Tsubota, Minoru S H Ko
Dry eye disease is the most prevalent pathological condition in aging eyes. One potential therapeutic strategy is the transplantation of lacrimal glands, generated in vitro from pluripotent stem cells such as human embryonic stem cells, into patients. One of the preceding requirements is a method to differentiate human embryonic stem cells into lacrimal gland epithelium cells. As the first step for this approach, this study aims to identify a set of transcription factors whose overexpression can promote the differentiation of human embryonic stem cells into lacrimal gland epithelium-like cells...
2017: NPJ Aging and Mechanisms of Disease
https://www.readbyqxmd.com/read/28649259/the-cbl-and-cipk-gene-family-in-grapevine-vitis-vinifera-genome-wide-analysis-and-expression-profiles-in-response-to-various-abiotic-stresses
#13
Yue Xi, Jinyi Liu, Chao Dong, Zong-Ming Max Cheng
Calcium plays a central role in regulating signal transduction pathways. Calcineurin B-like proteins (CBLs), which harbor a crucial region consisting of EF hands that capture Ca(2+), interact in a specific manner with CBL-interacting protein kinases (CIPKs). This two gene families or their interacting-complex widely respond to various environment stimuli and development processes. The genome-wide annotation and specific expression patterns of CBLs and CIPKs, however, in grapevine remain unclear. In the present study, eight CBL and 20 CIPK genes were identified in grapevine genome, and divided into four and five subfamilies, respectively, based on phylogenetic analysis, and validated by gene structure and the distribution of conserved protein motifs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28649126/cis-stilbene-glucoside-in-polygonum-multiflorum-induces-immunological-idiosyncratic-hepatotoxicity-in-lps-treated-rats-by-suppressing-ppar-%C3%AE
#14
Ya-Kun Meng, Chun-Yu Li, Rui-Yu Li, Lan-Zhi He, He-Rong Cui, Ping Yin, Cong-En Zhang, Peng-Yan Li, Xiu-Xiu Sang, Ya Wang, Ming Niu, Ya-Ming Zhang, Yu-Ming Guo, Rong Sun, Jia-Bo Wang, Zhao-Fang Bai, Xiao-He Xiao
The root of Polygonum multiflorum Thunb (PM) has been used in China to treat a variety of diseases, such as constipation, early graying of the hair and hyperlipemia. Recent evidence shows that PM causes idiosyncratic drug-induced liver injury (IDILI) in humans. In this study, we investigated the molecular basis of PM-induced liver injury in a rat model of IDILI based on a non-hepatotoxic dose of LPS. SD rats were orally administered 3 potentially hepatotoxic compounds of PM: cis-stilbene glucoside (cis-SG, 50 mg/kg), trans-SG (50 mg/kg) or emodin (5 mg/kg), followed by injection of LPS (2...
June 26, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28649092/aryl-hydrocarbon-receptor-ahr-is-a-potential-tumour-suppressor-in-pituitary-adenomas
#15
Robert Formosa, Joseph Borg, Josanne Vassallo
Pituitary adenomas (PA) represent the largest group of intracranial neoplasms, yet the molecular mechanisms driving this disease remain largely unknown. The aim of this study was to use high-throughput screening to identify molecular pathways that may be playing a significant and consistent role in PA. RNA profiling using microarrays on eight local PAs identified the aryl hydrocarbon receptor (AHR) signalling pathway as a key canonical pathway down-regulated in all PA types. This was confirmed by qPCR in 31 tumours...
June 25, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/28649033/danazol-alters-mitochondria-metabolism-of-fibrocystic-breast-mcf10a-cells
#16
Zhazira Irgebay, Banu Yeszhan, Bhaswati Sen, Sultan Tuleukhanov, Ari D Brooks, Richard Sensenig, Zulfiya Orynbayeva
Fibrocystic Breast Disease (FBD) or Fibrocystic change (FC) affects about 60% of women at some time during their life. Although usually benign, it is often associated with pain and tenderness (mastalgia). The synthetic steroid danazol has been shown to be effective in reducing the pain associated with FBD, but the cellular and molecular mechanisms for its action have not been elucidated. We investigated the hypothesis that danazol acts by affecting energy metabolism. Effects of danazol on Mcf10A cells homeostasis, including mechanisms of oxidative phosphorylation, cytosolic calcium signaling and oxidative stress, were assessed by high-resolution respirometry and flow cytometry...
June 22, 2017: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/28648804/the-single-igf-1-partial-deficiency-is-responsible-for-mitochondrial-dysfunction-and-is-restored-by-igf-1-replacement-therapy
#17
M Olleros Santos-Ruiz, M C Sádaba, I Martín-Estal, U Muñoz, C Sebal Neira, I Castilla-Cortázar
BACKGROUND & AIMS: We previously described in cirrhosis and aging, both conditions of IGF-1 deficiency, a clear hepatic mitochondrial dysfunction with increased oxidative damage. In both conditions, the hepatic mitochondrial function was improved with low doses of IGF-1. The aim of this work was to explore if the only mere IGF-1 partial deficiency, without any exogenous insult, is responsible for hepatic mitochondrial dysfunction. METHODS: Heterozygous (igf1(+/-)) mice were divided into two groups: untreated and treated mice with low doses of IGF-1...
June 2, 2017: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/28648760/corneal-endothelial-cells-activate-innate-and-acquired-arm-of-anti-viral-responses-after-cytomegalovirus-infection
#18
Dai Miyazaki, Ryu Uotani, Michiko Inoue, Tomoko Haruki, Yumiko Shimizu, Keiko Yakura, Satoru Yamagami, Tatsuo Suzutani, Mayumi Hosogai, Hiroki Isomura, Yoshitsugu Inoue
Infection of the corneal endothelial cells by human cytomegalovirus (CMV) is an important cause of corneal endotheliitis. CMV endotheliitis is difficult to completely cure and relapses are frequent. This can cause blinding corneal bullous keratopathy. However, the pathogenesis of CMV endotheliitis remains undetermined. To understand the immunopathology of endotheliitis, we examined how corneal endothelial cells prime the anti-viral immunity after CMV infection based on global transcriptional responses. To accomplish this, human corneal endothelial (HCEn) cells were infected with CMV, and the global transcriptional responses were determined by microarray analyses for primary anti-viral responses using network analysis...
June 22, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28648530/automatic-quantification-of-ihc-stain-in-breast-tma-using-colour-analysis
#19
M Milagro Fernández-Carrobles, Gloria Bueno, Marcial García-Rojo, Lucía González-López, Carlos López, Oscar Déniz
Immunohistochemical (IHC) biomarkers in breast tissue microarray (TMA) samples are used daily in pathology departments. In recent years, automatic methods to evaluate positive staining have been investigated since they may save time and reduce errors in the diagnosis. These errors are mostly due to subjective evaluation. The aim of this work is to develop a density tool able to automatically quantify the positive brown IHC stain in breast TMA for different biomarkers. To avoid the problem of colour variation and make a robust tool independent of the staining process, several colour standardization methods have been analysed...
June 13, 2017: Computerized Medical Imaging and Graphics: the Official Journal of the Computerized Medical Imaging Society
https://www.readbyqxmd.com/read/28648459/acute-liver-failure-is-associated-with-altered-cerebral-expression-profiles-of-long-non-coding-rnas
#20
Vinícius R Silva, Rodrigo Secolin, Raghu Vemuganti, Iscia Lopes-Cendes, Alan S Hazell
Hepatic encephalopathy (HE) represents a serious complication of acute liver failure (ALF) in which cerebral edema leading to brainstem herniation as a result of increased intracranial hypertension is a major consequence. Long non-coding RNAs (lncRNAs) play a significant role in coordinating gene expression, with recent studies indicating an influence in the pathogenesis of several diseases. To investigate their involvement in the cerebral pathophysiology of ALF, we profiled the expression of lncRNAs in the frontal cortex of mice at coma stage following treatment with the hepatotoxin azoxymethane...
June 22, 2017: Neuroscience Letters
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