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https://www.readbyqxmd.com/read/28239780/sesamin-extends-lifespan-through-pathways-related-to-dietary-restriction-in-caenorhabditis-elegans
#1
Yumiko Nakatani, Yukie Yaguchi, Tomomi Komura, Masakazu Nakadai, Kenji Terao, Eriko Kage-Nakadai, Yoshikazu Nishikawa
PURPOSE: Sesamin, a polyphenolic compound found in sesame seeds, has been reported to exert a variety of beneficial health effects. We have previously reported that sesamin increases the lifespan of Caenorhabditis elegans. In this study, we investigated the molecular mechanisms underlying the longevity effect of sesamin in C. elegans. METHODS: Starting from three days of age, Caenorhabditis elegans animals were fed a standard diet alone or supplemented with sesamin...
February 26, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28239692/high-throughput-screening-of-microchip-synthesized-genes-in-programmable-double-emulsion-droplets
#2
H F Chan, S Ma, J Tian, K W Leong
The rapid advances in synthetic biology and biotechnology are increasingly demanding high-throughput screening technology, such as screening of the functionalities of synthetic genes for optimization of protein expression. Compartmentalization of single cells in water-in-oil (W/O) emulsion droplets allows screening of a vast number of individualized assays, and recent advances in automated microfluidic devices further help realize the potential of droplet technology for high-throughput screening. However these single-emulsion droplets are incompatible with aqueous phase analysis and the inner droplet environment cannot easily communicate with the external phase...
February 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28239559/gene-profiling-the-window-of-implantation-microarray-analyses-from-human-and-rodent-models
#3
Jennifer L Herington, Yan Guo, Jeff Reese, Bibhash C Paria
Poor uterine receptivity leads to implantation defects or failure. Identification of uterine molecules crucial to uterine receptivity and/or embryo implantation provides the opportunity to design a diagnostic screening toolkit for uterine receptivity or targeted drug discovery for treating implantation-based infertility. In this regard, gene-profiling studies performed in humans and rodents have identified numerous genes involved in the transcriptional regulation of uterine receptivity and embryo implantation...
December 2016: J Reprod Health Med
https://www.readbyqxmd.com/read/28239339/microarray-and-qpcr-analyses-of-wallerian-degeneration-in-rat-sciatic-nerves
#4
Sheng Yi, Xin Tang, Jun Yu, Jie Liu, Fei Ding, Xiaosong Gu
Wallerian degeneration occurs immediately following injury to mammal peripheral nerves. To better understand the molecular events occurring during Wallerian degeneration, a rat model of sciatic nerve transection was used to assess differentially expressed genes at 0.5, 1, 6, 12, 24 h, 4 days, 1, 2, 3, and 4 weeks post nerve injury (PNI). Hierarchical clustering, Euclidean distance matrix, and principal component analysis (PCA) collectively suggested three distinct phases within the post-injury period of 4 weeks...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28239029/comprehensive-analysis-of-gene-mutation-and-expression-profiles-in-neuroendocrine-carcinomas-of-the-stomach
#5
Rie Makuuchi, Masanori Terashima, Masatoshi Kusuhara, Takashi Nakajima, Masakuni Serizawa, Keiichi Hatakeyama, Keiichi Ohshima, Kenichi Urakami, Ken Yamaguchi
The gene mutation and expression profiles of gastric neuroendocrine carcinoma (NEC) have not been comprehensively determined. Here, we examined the gene mutation and expression profiles of NEC using whole exome sequencing (WES) and microarray analysis. Six patients with gastric NEC and 13 with gastric adenocarcinoma (GAD) were included in this study. Single nucleotide variants were compared and multivariate statistical investigation with orthogonal partial least squares discriminant analysis (OPLS-DA) was performed to compare the difference in expression profiles between NEC and GAD...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28238761/agr2-associates-with-her2-expression-predicting-poor-outcome-in-subset-of-estrogen-receptor-negative-breast-cancer-patients
#6
Eva Ondrouskova, Lucia Sommerova, Rudolf Nenutil, Oldrich Coufal, Pavel Bouchal, Borivoj Vojtesek, Roman Hrstka
Expression of the AGR2 oncogene was shown to be associated with estrogen receptor positive tumors. This gene contributes to enhanced cellular proliferation, drug resistance, metastasis development and may also serve as a predictor of poor prognosis. However, our analysis of AGR2 expression in a subset of estrogen-receptor negative tumors revealed that AGR2 could also be upregulated in hormone-independent manner. AGR2 expression was shown to be significantly increased in HER2 positive breast tumors on both the mRNA and the protein level...
February 23, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28238728/an-integrative-transcriptomic-analysis-reveals-bisphenol-a-exposure-induced-dysregulation-of-microrna-expression-in-human-endometrial-cells
#7
Wei-Chun Chou, Pei-Hsuan Lee, Yan-Yan Tan, Ho-Chen Lin, Chung-Wei Yang, Kuan-Hsueh Chen, Chun-Yu Chuang
Bisphenol A (BPA) are commonly used in the manufacture of polycarbonate plastics. Higher BPA exposure levels have been found in patients with endometrial hyperplasia that is one of risk factors of endometrial cancer (EC). Aberrant microRNAs (miRNAs) regulation has been observed in the development of cancer. Thus, this study investigated whether BPA exposure can disrupt miRNA regulation and its gene expression regarding to EC carcinogenic progress. Microarray experiments of miRNA and mRNA were performed in human endometrial cancer RL95-2 cells with treatment of low-to-moderate (10, 10(3) and 10(5)nM) BPA to explore the aberrant genes corresponding to human EC progression...
February 23, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28238417/enhanced-expression-of-programmed-cell-death-1-pd-1-protein-in-benign-vascular-anomalies
#8
Clarissa N Amaya, Frank H Wians, Brad A Bryan, Alireza Torabi
Programmed cell death 1 (PD-1) and its ligands have been shown to play a significant role in evasion of malignant tumour cells from the immune system. Last year, the United States Food and Drug Administration (FDA) approved anti-PD-1 inhibitors for treatment of non-small cell lung carcinoma and recently has approved anti-PD-L1 blocker for treatment of metastatic urothelial cell carcinoma. However, the role that the immune system might have on benign tumours including vascular anomalies has received less attention...
February 23, 2017: Pathology
https://www.readbyqxmd.com/read/28238352/reliable-mite-specific-ige-testing-in-nasal-secretions-by-means-of-allergen-microarray
#9
Margot Berings, Stefania Arasi, Natalie De Ruyck, Serena Perna, Yvonne Resch, Christian Lupinek, Kuan-Wei Chen, Susanne Vrtala, Giovanni Battista Pajno, Claus Bachert, Bart N Lambrecht, Melissa Dullaers, Rudolf Valenta, Paolo Maria Matricardi, Philippe Gevaert
No abstract text is available yet for this article.
February 18, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28238255/linear-multi-epitope-glyco-peptides-for-type-specific-serology-of-herpes-simplex-virus-hsv-infections
#10
Christian Walter Risinger, Kasper Kildegaard Sørensen, Knud J Jensen, Sigvard Olofsson, Tomas Bergstrom, Ola Blixt
Detection of type-specific antibodies is an important and essential part of accurate diagnosis, even in silent carriers of HSV-1 (oral) and HSV-2 (genital) infections. Serologic assays that identify HSV-1 and HSV-2 type-specific antibodies have been commercially available for more than a decade but often face problems related to cross-reactivity and similar issues. Attempts to identify type specific peptide epitopes for use in serology for both HSV-1 and HSV-2 have been limited. We recently demonstrated epitope mapping of envelope glycoprotein G2 and identified a type-specific glycopeptide epitope that broadly recognized HSV-2 infected individuals...
February 26, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28237701/regulator-of-g-protein-signaling-20-correlates-with-clinicopathological-features-and-prognosis-in-triple-negative-breast-cancer
#11
Quan Li, Wenxu Jin, Yefeng Cai, Fang Yang, Endong Chen, Danrong Ye, Qingxuan Wang, Xiaoxiang Guan
Triple-negative breast cancer (TNBC) is a highly aggressive tumor subtype lacking effective prognostic indicators or therapeutic targets. Therefore, finding a novel molecular biomarker for TNBC to achieve target therapy and predict its prognosis is crucial in preventing inappropriate treatment. Regulator of G-protein signaling (RGS) families of protein can negatively regulate signaling of heterotrimeric G proteins and are known to be upregulated in various tumors. In this study, we demonstrated that RGS20 was more highly expressed in TNBC tumor tissue than in adjacent normal tissue by analyzing the cancer genome atlas (TCGA) database...
February 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28237528/identification-of-potential-therapeutic-target-genes-key-mirnas-and-mechanisms-in-oral-lichen-planus-by-bioinformatics-analysis
#12
Cuihua Gong, Shangtong Sun, Bing Liu, Jing Wang, Xiaodong Chen
The study aimed to identify the potential target genes and key miRNAs as well as to explore the underlying mechanisms in the pathogenesis of oral lichen planus (OLP) by bioinformatics analysis. The microarray data of GSE38617 were downloaded from Gene Expression Omnibus (GEO) database. A total of 7 OLP and 7 normal samples were used to identify the differentially expressed genes (DEGs) and miRNAs. The DEGs were then performed functional enrichment analyses. Furthermore, DEG-miRNA network and miRNA-function network were constructed by Cytoscape software...
February 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28237325/the-effect-of-lysophosphatidic-acid-together-with-interferon-tau-on-the-global-transcriptomic-profile-in-bovine-endometrial-cells
#13
Dorota Boruszewska, Ilona Kowalczyk-Zieba, Emilia Sinderewicz, Katarzyna Grycmacher, Joanna Staszkiewicz, Izabela Woclawek-Potocka
In cows, lysophosphatidic acid (LPA), which acts in an auto/paracrine manner, serves as a luteotropic factor during early pregnancy by stimulating progesterone and prostaglandin E2 secretion, thus protecting the bovine corpus luteum and early embryo development. Our hypothesis was that LPA exerted some local effects on the bovine endometrium prior to early embryo-maternal interactions and that interferon tau (IFNτ), the pregnancy recognition signal, modulated this action. In the present study, we applied an in vitro model involving whole-transcriptomic profiling to examine the effects of LPA on gene expression in bovine endometrial cells...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28237042/expression-of-ngf-gdnf-and-mmp-9-in-prostate-carcinoma
#14
Sirin Baspinar, Sema Bircan, Metin Ciris, Nermin Karahan, Kemal Kursat Bozkurt
The aim of the present study was to investigate the immunohistochemical expression of NGF, GDNF and MMP-9 in benign prostatic hyperplasia (BPH), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PC), and to analyse their association with the clinicopathological parameters in PC cases. Immunohistochemistry was performed on the tissue microarray (TMA) sections of 30 BPH, 40 HGPIN and 121 primary PC tissues. There was a significant difference regarding the expression of NGF and GDNF between PC and HGPIN (p<0...
February 10, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28236279/oas-gene-family-expression-is-associated-with-hiv-related-neurocognitive-disorders
#15
C Sanfilippo, M R Pinzone, D Cambria, A Longo, M Palumbo, R Di Marco, F Condorelli, G Nunnari, L Malaguarnera, M Di Rosa
HIV-associated neurocognitive disorders are common in HIV-infected individuals, even in the combination antiretroviral therapy (c-ART) era. Several mechanisms are involved in neuronal damage, including chronic inflammation immune activation. Mammalian 2'-5'-oligoadenylate synthetase (OAS) genes are produced in response to interferon (IFN), mainly by monocytes, and exert their antiviral functions by activation of RNase L that degrades viral and cellular RNAs. In this study, we aimed at exploring OAS gene family RNA expression in simian immunodeficiency virus encephalitis (SIVE), in HIV-associated neurocognitive disorders (HAND), and in HIV-associate dementia (HAD)...
February 24, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28236118/expression-of-vista-correlated-with-immunosuppression-and-synergized-with-cd8-to-predict-survival-in-human-oral-squamous-cell-carcinoma
#16
Lei Wu, Wei-Wei Deng, Cong-Fa Huang, Lin-Lin Bu, Guang-Tao Yu, Liang Mao, Wen-Feng Zhang, Bing Liu, Zhi-Jun Sun
V-domain Ig suppressor of T cell activation (VISTA), a novel immune checkpoint regulatory molecule, suppresses T cell mediated immune responses. The aim of the present study was to profile the immunological expression, clinical significance and correlation of VISTA in human oral squamous cell carcinoma (OSCC). Human tissue microarrays, containing 165 primary OSCCs, 48 oral epithelial dysplasias and 43 normal oral mucosae, were applied to investigate the expression levels of VISTA, CD8, cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), programmed death ligand 1 (PD-L1), PI3Kα p110, IL13Rα2, phospho-STAT3 at tyrosine 705 (p-STAT3) and myeloid-derived suppressor cell (MDSC) markers (CD11b and CD33) by immunohistochemistry and digital pathology analysis...
February 24, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28236054/gene-expression-signature-for-early-prediction-of-late-occurring-pancytopenia-in-irradiated-baboons
#17
Matthias Port, Francis Hérodin, Marco Valente, Michel Drouet, Andreas Lamkowski, Matthäus Majewski, Michael Abend
Based on gene expression changes measured in the peripheral blood within the first 2 days after irradiation, we predicted a pancytopenia in a baboon model. Eighteen baboons were irradiated with 2.5 or 5 Gy. According to changes in blood cell counts, the surviving baboons (n = 17) exhibited a hematological acute radiation syndrome (HARS) either with or without a pancytopenia. We used a two stage study design where stage I was a whole genome screen (microarrays) for mRNA combined with a qRT-PCR platform for simultaneous detection of 667 miRNAs using a part of the samples...
February 24, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28235844/tbrd-1-and-tbrd-2-regulate-expression-of-genes-necessary-for-spermatid-differentiation
#18
Ina Theofel, Marek Bartkuhn, Thomas Boettger, Stefanie M K Gärtner, Judith Kreher, Alexander Brehm, Christina Rathke
Male germ cell differentiation proceeds to a large extent in the absence of active gene transcription. In Drosophila, hundreds of genes whose proteins are required during post-meiotic spermatid differentiation (spermiogenesis) are transcribed in primary spermatocytes. Transcription of these genes depends on the sequential action of the testis meiotic arrest complex (tMAC), Mediator complex, and testis-specific TFIID (tTFIID) complex. How the action of these protein complexes is coordinated and which other factors are involved in the regulation of transcription in spermatocytes is not well understood...
February 24, 2017: Biology Open
https://www.readbyqxmd.com/read/28235791/high-throughput-screening-identifies-micrornas-that-target-nox2-and-improve-function-following-acute-myocardial-infarction
#19
Junyu Yang, Milton E Brown, Hanshuo Zhang, Mario Martinez, Zhihua Zhao, Srishti Bhutani, Shenyi Yin, David Trac, Jianzhong Jeff Xi, Michael E Davis
AIMS: Myocardial infarction (MI) is the most common cause of heart failure. Excessive production of reactive oxygen species plays a key role in the pathogenesis of cardiac remodeling after MI. NADPH with Nox2 as the catalytic subunit is a major source of superoxide production and expression is significantly increased in the infarcted myocardium, especially by infiltrating macrophages. While microRNAs (miRNAs) are potent regulators of gene expression, and play an important role in heart disease, there still lacks efficient ways to identify miRNAs that target important pathological genes for treating MI...
February 24, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28235630/comprehensive-immunohistochemical-analysis-of-histone-deacetylases-in-pancreatic-neuroendocrine-tumors-hdac5-as-a-predictor-of-poor-clinical-outcome
#20
Eckhard Klieser, Romana Urbas, Stefan Stättner, Florian Primavesi, Tarkan Jäger, Adam Dinnewitzer, Christian Mayr, Tobias Kiesslich, Klaus Holzmann, Pietro Di Fazio, Daniel Neureiter, Stefan Swierczynski
Epigenetic factors contribute to carcinogenesis, tumor promotion and chemoresistance. Histone deacetylases (HDACs) are epigenetic regulators that primarily cause chromatin compaction, leading to inaccessibility of promoter regions and eventually gene silencing. Many cancer entities feature over-expression of HDACs. Currently, the role of HDACs in pancreatic neuroendocrine tumors (pNETs) is unclear. We analyzed expression patterns of all HDAC classes (Class I, IIA, IIB, III & IV) in five human tissue microarrays (TMA) representing 57 pNETs resected between 1997 and 2013 and corresponding control tissue...
February 21, 2017: Human Pathology
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