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Minimum inhibitory concentration

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https://www.readbyqxmd.com/read/28431915/synergistic-anticandidal-activity-of-menthol-in-combination-with-itraconazole-and-nystatin-against-clinical-candida-glabrata-and-candida-krusei-isolates
#1
Aghil Sharifzadeh, Ali Reza Khosravi, Hojjatollah Shokri, Paria Samadi Tari
BACKGROUND: Candida glabrata (C. glabrata) and C. krusei are now emerging as serious hospital acquired infections in immunocompromised patients. Menthol, a terpenic compound, has been reported to have antifungal activity. OBJECTIVES: The aim of this study was to investigate the effect of menthol in combination with itraconazole or nystatin against C. glabrata and C. krusei isolates. METHODS: The effects of menthol along with itraconazole and nystatin, were evaluated by the Clinical Laboratory Standards Institute (CLSI) M44-A and CLSI M27-A3 methods...
April 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28431183/efficacy-of-tiamulin-alone-or-in-combination-with-chlortetracycline-against-experimental-mycoplasma-gallisepticum-infection-in-chickens
#2
A Garmyn, M Vereecken, K Degussem, W Depondt, F Haesebrouck, A Martel
Mycoplasma gallisepticum (M. gallisepticum) remains one of the most important diseases in poultry production. Controlling the impact of the disease is done by eradication of positive breeder flocks or by vaccination and medication. A widely used molecule in medication programs is tiamulin, a pleuromutilin antibiotic. Since recent data on the in vivo efficacy of this molecule are scarce, 2 challenge studies were conducted using a recently isolated M. gallisepticum strain belonging to the wildtype population with regard to its tiamulin and tetracycline minimum inhibitory concentration (MIC)...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28429168/bedaquiline-susceptibility-test-for-totally-drug-resistant-tuberculosis-mycobacterium-tuberculosis
#3
Ji-Chan Jang, Yong-Gyun Jung, Jungil Choi, Hyunju Jung, Sungweon Ryoo
This study aimed to provide information that bedaquilline is significantly effective for treatment of totally drug resistant (TDR) Mycobacterium tuberculosis that shows resistant to all first- and second-line drugs-using an innovative disc agarose channel (DAC) system. Time-lapse images of single bacterial cells under culture conditions with different concentrations of bedaquiline were analysed by image processing software to determine minimum inhibitory concentrations (MICs). Bedaquiline inhibited the growth of TDR M...
April 20, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28428132/novel-properties-of-hippophae-rhamnoides-l-twig-and-leaf-extracts-anti-virulence-action-and-synergy-with-antifungals-studied-in-vitro-on-candida-spp-model
#4
Beata Sadowska, Aleksandra Budzyńska, Anna Stochmal, Jerzy Żuchowski, Barbara Różalska
Original, chemically characterized Sea buckthorn (SBT) twig and leaf extracts were in vitro studied in terms of anti-Candida activity. Minimum inhibitory concentrations (MICs) of the extracts against C. albicans ATCC 10231 ranged: 250 μg/ml (twig), 31.5 μg/ml (leaf), and against C. glabrata G1 (clinical isolate) - 15.6 μg/ml (twig), 3.9 μg/ml (leaf). Next the extracts have been used at their subMIC. Both extracts significantly enhanced activity of fluconazole (FLC) and caspofungin (CAS) against C. albicans and increased their efficacy against C...
April 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28427461/inhibitory-activities-of-selected-sudanese-medicinal-plants-on-porphyromonas-gingivalis-and-matrix-metalloproteinase-9-and-isolation-of-bioactive-compounds-from-combretum-hartmannianum-schweinf-bark
#5
Ebtihal Abdalla M Mohieldin, Ali Mahmoud Muddathir, Tohru Mitsunaga
BACKGROUND: Periodontal diseases are one of the major health problems and among the most important preventable global infectious diseases. Porphyromonas gingivalis is an anaerobic Gram-negative bacterium which has been strongly implicated in the etiology of periodontitis. Additionally, matrix metalloproteinases-9 (MMP-9) is an important factor contributing to periodontal tissue destruction by a variety of mechanisms. The purpose of this study was to evaluate the selected Sudanese medicinal plants against P...
April 20, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28425874/in-vitro-and-in-vivo-assessments-of-rhodomyrtus-tomentosa-leaf-extract-as-an-alternative-anti-streptococcal-agent-in-nile-tilapia-oreochromis-niloticus-l
#6
Pinanong Na-Phatthalung, Sasitorn Chusri, Naraid Suanyuk, Supayang Piyawan Voravuthikunchai
PURPOSE: Rhodomyrtustomentosa is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of R. tomentosa leaf extract against Streptococcus agalactiae and Streptococcus iniae isolated from infected tilapia. METHODOLOGY: The anti-streptococcal activity of R. tomentosa was determined using broth microdilution assays...
April 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28424995/the-emergence-of-vancomycin-resistant-staphylococcus-aureus-in-an-intensive-care-unit-in-kerman-iran
#7
Yasser Fasihi, Fereshteh Saffari, Shahla Mansouri, Davood Kalantar-Neyestanaki
Methicillin-resistant Staphylococcus aureus (MRSA) is a global threat to public health. This study is the first report of the emergence of vancomycin-resistant MRSA in Kerman, Iran. During a period of 15 months, a total of 205 clinical isolates of S. aureus were collected from three university hospitals affiliated with the Kerman University of Medical Science, Kerman, Iran. Screening of methicillin and vancomycin resistance was carried out by phenotypic methods. The resistance and virulence genes of vancomycin-resistant isolates were detected by polymerase chain reaction (PCR)...
April 19, 2017: Wiener Medizinische Wochenschrift
https://www.readbyqxmd.com/read/28422479/on-demand-gas-to-liquid-process-to-fabricate-thermoresponsive-antimicrobial-nanocomposites-and-coatings
#8
Bijay Kumar Poudel, Jae Hong Park, Jeong Hoon Byeon
Antimicrobial material is emerging as a major component of the mitigation strategy against microbial growth on abiotic surfaces. In this work, a newly designed process is proposed to fabricate thermoresponsive antimicrobial nanocomposites (TANs) and coatings (TACs) as an on-demand system. Thermoresponsive polymer (TRP)-incorporated silver (Ag) nanocomposites with silica nanoparticles (SNPs) or carbon nanotubes (CNTs; Ag-SNP@TRP or Ag-CNT@TRP) were produced by a single-pass gas-to-liquid process. The SNPs or CNTs were first produced by spark ablation and successively injected for dispersal in a liquid cell containing polydimethylsiloxane, poly(N-isopropylacrylamide), and silver nitrate under ultrasound irradiation...
April 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28422057/antifungal-and-anti-biofilm-activities-of-acetone-lichen-extracts-against-candida-albicans
#9
Marion Millot, Marion Girardot, Lucile Dutreix, Lengo Mambu, Christine Imbert
Candida albicans is a commensal coloniser of the human gastrointestinal tract and an opportunistic pathogen, especially thanks to its capacity to form biofilms. This lifestyle is frequently involved in infections and increases the yeast resistance to antimicrobials and immune defenses. In this context, 38 lichen acetone extracts have been prepared and evaluated for their activity against C. albicans planktonic and sessile cells. Minimum inhibitory concentrations of extracts (MICs) were determined using the broth microdilution method...
April 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28422054/growth-inhibition-of-sulfate-reducing-bacteria-in-produced-water-from-the-petroleum-industry-using-essential-oils
#10
Pamella Macedo de Souza, Fátima Regina de Vasconcelos Goulart, Joana Montezano Marques, Humberto Ribeiro Bizzo, Arie Fitzgerald Blank, Claudia Groposo, Maíra Paula de Sousa, Vanessa Vólaro, Celuta Sales Alviano, Daniela Sales Alviano Moreno, Lucy Seldin
Strategies for the control of sulfate-reducing bacteria (SRB) in the oil industry involve the use of high concentrations of biocides, but these may induce bacterial resistance and/or be harmful to public health and the environment. Essential oils (EO) produced by plants inhibit the growth of different microorganisms and are a possible alternative for controlling SRB. We aimed to characterize the bacterial community of produced water obtained from a Brazilian petroleum facility using molecular methods, as well as to evaluate the antimicrobial activity of EO from different plants and their major components against Desulfovibrio alaskensis NCIMB 13491 and against SRB growth directly in the produced water...
April 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28421415/a-tyrosine-residue-along-with-a-glutamic-acid-of-the-omega-like-loop-governs-the-beta-lactamase-activity-of-msmeg_4455-in-mycobacterium-smegmatis
#11
Ankita Bansal, Debasish Kar, Satya Deo Pandey, Ashok Matcha, N Ganesh Kumar, Soshina Nathan, Anindya S Ghosh
Mycobacterial beta-lactamases are involved in exerting beta-lactam resistance, though many of these proteins remain uncharacterized. Here, we have characterized MSMEG_4455 of Mycobacterium smegmatis as a beta-lactamase using molecular, biochemical and mutational techniques. To elucidate its nature in vivo and in vitro, and to predict its structure-function relationship in silico analysis is done. The MSMEG_4455 is cloned and expressed ectopically in a beta-lactamase deficient Escherichia coli mutant to establish the in vivo beta-lactamase like nature via minimum inhibitory concentration (MIC) determination...
April 19, 2017: Protein Journal
https://www.readbyqxmd.com/read/28418717/diversity-of-endophytic-bacteria-in-a-fern-species-dryopteris-uniformis-makino-makino-and-evaluation-of-their-antibacterial-potential-against-five-foodborne-pathogenic-bacteria
#12
Gitishree Das, Seonjoo Park, Kwang-Hyun Baek
The fern plant Dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. This study was conducted to explore the endophytic bacterial diversity associated with D. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (FPB). Among 51 isolated endophytic bacteria (EB), 26 EB were selected based on their morphological characteristics and identified by 16S rRNA gene analysis. The distribution of EB was diverse in the leaf and the stem/root tissues...
April 18, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28418601/to-arginine-residues-in-the-cooh-terminal-of-human-%C3%AE-defensin-3-constitute-an-essential-motif-for-antimicrobial-activity-and-il-6-production
#13
Yoko Sakagami-Yasui, Yoshinori Shirafuji, Osamu Yamasaki, Shin Morizane, Toshihisa Hamada, Hiroshi Umemura, Keiji Iwatsuki
Human β-defensin-3 (HBD-3) possesses antimicrobial activities and the potential to induce proinflammatory cytokines. HBD-3 contains a unique motif of two arginine residues (Arg. R) in the COOH-terminal region. To understand the bioactive properties of the Arg residues of HBD-3, we examined antimicrobial activities against S. aureus and P. aeruginosa using synthetic HBD-2, HBD-3, and two variant peptides of HBD-3: the Arg-truncated variant designated desR HBD-3, and NRR HBD-3, in which both Arg residues were shifted to the N-terminal region...
April 18, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28417918/high-prevalence-of-blandm-1-blavim-qace-and-qace%C3%AE-1-genes-and-their-association-with-decreased-susceptibility-to-antibiotics-and-common-hospital-biocides-in-clinical-isolates-of-acinetobacter-baumannii
#14
Fatma Alzahraa M Gomaa, Zeinab H Helal, Mazhar I Khan
The objective of this study was to evaluate the susceptibility of metallo-β-lactamase (MBL)-producing Acinetobacter baumannii (A. baumannii) clinical isolates to biocides. We also determined the prevalence and correlation of efflux pump genes, class 1 integron and MBL encoding genes. In addition, blaVIM, blaNDM-1, qacE and qacEΔ1 nucleotide sequence analysis was performed and compared to sequences retrieved from GenBank at the National Center for Biotechnology Information database. A. baumannii had a resistance rate to carbapenem of 71...
April 12, 2017: Microorganisms
https://www.readbyqxmd.com/read/28417524/biofilms-of-candida-spp-from-the-ocular-conjunctiva-of-horses-with-reduced-azole-susceptibility-a-complicating-factor-for-the-treatment-of-keratomycosis
#15
Raimunda Sâmia Nogueira Brilhante, Paula Vago Bittencourt, Débora de Souza Collares Castelo-Branco, Glaucia Morgana de Melo Guedes, Jonathas Sales de Oliveira, Lucas Pereira Alencar, Rossana de Aguiar Cordeiro, Mariana Pinheiro, Evilázio Fernandes Nogueira-Filho, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
OBJECTIVES: This study aimed to assess the biofilm-forming ability of Candida spp. from the ocular conjunctiva of horses and to investigate the antifungal susceptibility of these biofilms. PROCEDURES: Initially, the biofilm-forming ability of 15 strains was assessed by crystal violet staining, which reveals the fungal biomass adhered to the polystyrene plates, and scanning electron microscopy. Then, the minimum inhibitory concentrations (MICs) of amphotericin B, fluconazole, itraconazole, and caspofungin were initially determined against strains in planktonic form...
April 18, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28415524/biomolecule-assisted-synthesis-of-ag-reduced-graphene-oxide-nanocomposite-with-excellent-electrocatalytic-and-antibacterial-performance
#16
Soghra Fathalipour, Sima Pourbeyram, Aziaeh Sharafian, Asghar Tanomand, Parisa Azam
In this work, an environmentally friendly method was applied for the synthesis of aqueous suspension of l-cysteine modified Ag nanoparticles (NPs)-decorated reduced graphene oxide (rGO) nanocomposite. l-cysteine played a triple role as reducing agent, stabilizer and linker of Ag NPs onto the surface of rGO. The resultant nanocomposite was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction studies (XRD), zeta potential, Raman spectroscopy, scanning electron microscopy (SEM) and energy dispersive analysis of X-ray (EDX)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415502/antimicrobial-textiles-biogenic-silver-nanoparticles-against-candida-and-xanthomonas
#17
Daniela Ballottin, Stephanie Fulaz, Flávia Cabrini, Junko Tsukamoto, Nelson Durán, Oswaldo L Alves, Ljubica Tasic
This paper introduces cotton fibers impregnated with biogenic silver nanoparticles (AgNPs), synthesized from a Fusarium oxysporum fungal filtrate (FF) solution, and open up the possibility for their use in medical environment and agriculture clothing as means to avoid microbial spreading. After thorough AgNPs characterization, regarding their physical, chemical and biochemical properties, Minimum Inhibitory Concentrations (MIC) against some human and orange tree pathogens were determined. We report the strong AgNPs activity against Candida parapsilosis and Xanthomonas axonopodis pv...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415462/microencapsulation-of-eugenol-molecules-by-%C3%AE-cyclodextrine-as-a-thermal-protection-method-of-antibacterial-action
#18
R Piletti, A M Bugiereck, A T Pereira, E Gussati, J Dal Magro, J M M Mello, F Dalcanton, R Z Ternus, C Soares, H G Riella, M A Fiori
Eugenol is natural oil that has excellent antibacterial properties but cannot be used to fabricate many products that require thermal processing. One possible alternative to the use of the eugenol molecules in high-temperature processes is the encapsulation of these molecules in a structure that is not toxic and is resistant to thermal treatment. This work investigated the encapsulation process of eugenol molecules in β-cyclodextrine and the antibacterial properties of eugenol-β-cyclodextrine (the eugenol-βCD complex) against Escherichia coli and Staphylococcus aureus...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415415/curcumin-loaded-polysaccharide-nanoparticles-optimization-and-anticariogenic-activity-against-streptococcus-mutans
#19
Amir Maghsoudi, Fatemeh Yazdian, Saleheh Shahmoradi, Leila Ghaderi, Mehran Hemati, Ghassem Amoabediny
Curcumin was loaded into different polysaccharide nanoparticles chitosan, alginate and starch, using the desolvation method. Curcumin-loaded nanoparticles exhibited enhanced solubility in aqueous solutions comparing with free curcumin. Effects of formulation parameters such as curcumin concentration and different volumes of ethanolic solution were affected the particle size and loading efficiency. Under optimum conditions, curcumin-loaded chitosan, starch and alginate nanoparticles with mean particles sizes of 66...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415384/preparation-and-characterization-of-expanded-graphite-metal-oxides-for-antimicrobial-application
#20
Wei-Che Hung, Kuo-Hui Wu, Dong-Yi Lyu, Ken-Fa Cheng, Wen-Chien Huang
Composite materials based on expanded graphite (EG) and metal oxide (MO) particles was prepared by an explosive combustion and blending method. The objective of the study was to develop EG impregnated with metal oxide particulates (Ag2O, CuO and ZnO) and evaluate the level of protection the materials conferred against biological agents. The physical properties of the EG/MO composites were examined using SEM, EDX and XRD spectroscopy, and the results indicated that the MO particles were incorporated into the EG matrix after impregnation...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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