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"Intracellular water"

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https://www.readbyqxmd.com/read/29338044/probing-the-changes-in-gene-expression-due-to-%C3%AE-crystallin-mutations-in-mouse-models-of-hereditary-human-cataract
#1
Usha P Andley, Eric Tycksen, Brittney N McGlasson-Naumann, Paul D Hamilton
The mammalian eye lens expresses a high concentration of crystallins (α, β and γ-crystallins) to maintain the refractive index essential for lens transparency. Crystallins are long-lived proteins that do not turnover throughout life. The structural destabilization of crystallins by UV exposure, glycation, oxidative stress and mutations in crystallin genes leads to protein aggregation and development of cataracts. Several destabilizing mutations in crystallin genes are linked with human autosomal dominant hereditary cataracts...
2018: PloS One
https://www.readbyqxmd.com/read/29212765/changes-in-the-fluid-volume-balance-between-intra-and-extracellular-water-in-a-sample-of-japanese-adults-aged-15-88-years-old-a-cross-sectional-study
#2
Yasushi Ohashi, Nobuhiko Joki, Keisuke Yamazaki, Takeshi Kawamura, Reibin Tai, Hideyo Oguchi, Rena Yuasa, Ken Sakai
The fluid volume balance between intracellular water (ICW) and extracellular water (ECW) gradually changes with age and various medical conditions. Comprehension of these physiological changes would aid in clinical decision-making related to body fluid assessments. A total of 1,992 individuals (753 men and 1,239 women) aged ≥15 years included in this study had their body composition measurements performed at training gyms in 2014. We developed a regression formula to assess the association of age with the ratio of ECW to ICW in these subjects...
December 6, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29198074/comparison-of-muscle-lean-mass-measurement-methods-correlation-with-functional-and-biochemical-testing
#3
B Buehring, E Siglinsky, D Krueger, W Evans, M Hellerstein, Y Yamada, N Binkley
DXA-measured lean mass is often used to assess muscle mass but has limitations. Thus, we compared DXA lean mass with two novel methods-bioelectric impedance spectroscopy and creatine (methyl-d3) dilution. The examined methodologies did not measure lean mass similarly and the correlation with muscle biomarkers/function varied. INTRODUCTION: Muscle function tests predict adverse health outcomes better than lean mass measurement. This may reflect limitations of current mass measurement methods...
December 2, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/29182426/effects-of-single-set-resistance-training-with-different-frequencies-on-a-cellular-health-indicator-in-older-women
#4
Alex S Ribeiro, Matheus A Nascimento, Brad J Schoenfeld, João Pedro Nunes, Andreo F Aguiar, Edilaine F Cavalcante, Analiza M Silva, Luís B Sardinha, Steven J Fleck, Edilson S Cyrino
The main purpose of this study was compare the effects of resistance training (RT) performed two versus three times per week on phase angle (a cellular health indicator) in older women. Thirty-nine women (69.1±5.5 years) were randomly assigned to perform a RT program two (G2X) or three (G3X) days per week for 12 weeks. The RT was a whole-body program (8 exercises, 1 set, 10-15 repetitions). Phase angle, resistance, reactance, and total body water were assessed by bioimpedance spectroscopy. Intracellular water, reactance, and phase angle increased significantly in G2X (2...
November 28, 2017: Journal of Aging and Physical Activity
https://www.readbyqxmd.com/read/29176686/the-dynamics-of-intracellular-water-constrains-glycolytic-oscillations-in-saccharomyces-cerevisiae
#5
Henrik S Thoke, Sigmundur Thorsteinsson, Roberto P Stock, Luis A Bagatolli, Lars F Olsen
We explored the dynamic coupling of intracellular water with metabolism in yeast cells. Using the polarity-sensitive probe 6-acetyl-2-dimethylaminonaphthalene (ACDAN), we show that glycolytic oscillations in the yeast S. cerevisiae BY4743 wild-type strain are coupled to the generalized polarization (GP) function of ACDAN, which measures the physical state of intracellular water. We analysed the oscillatory dynamics in wild type and 24 mutant strains with mutations in many different enzymes and proteins. Using fluorescence spectroscopy, we measured the amplitude and frequency of the metabolic oscillations and ACDAN GP in the resting state of all 25 strains...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29170709/effects-of-heat-exposure-on-body-water-assessed-using-single-frequency-bioelectrical-impedance-analysis-and-bioimpedance-spectroscopy
#6
Brett S Nickerson, Michael R Esco, Phillip A Bishop, Brian M Kliszczewicz, Henry N Williford, Kyung-Shin Park, Bailey A Welborn, Ronald L Snarr, Danilo V Tolusso
The purpose of this study was to determine if heat exposure alters the measures of total body water (TBW), extracellular water (ECW), and intracellular water (ICW) in both single-frequency bioelectrical impedance analysis (BIA) and bioimpedance spectroscopy (BIS). Additionally, we sought to determine if any differences exist between the BIA and BIS techniques before and after brief exposure to heat. Body water was evaluated for twenty men (age=24±4 years) in a thermoneutral environment (22°C) before (PRE) and immediately after (POST) 15 min of passive heating (35°C) in an environmental chamber...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/29158619/the-effect-of-mineral-based-alkaline-water-on-hydration-status-and-the-metabolic-response-to-short-term-anaerobic-exercise
#7
Jakub Chycki, Tomasz Zając, Adam Maszczyk, Anna Kurylas
Previously it was demonstrated that mineralization and alkalization properties of mineral water are important factors influencing acid-base balance and hydration in athletes. The purpose of this study was to investigate the effects of drinking different types of water on urine pH, specific urine gravity, and post-exercise lactate utilization in response to strenuous exercise. Thirty-six male soccer players were divided into three intervention groups, consuming around 4.0 l/day of different types of water for 7 days: HM (n=12; highly mineralized water), LM (n=12; low mineralized water), and CON (n=12; table water)...
September 2017: Biology of Sport
https://www.readbyqxmd.com/read/29146716/dynamic-contrast-enhanced-mri-derived-intracellular-water-lifetime-%C3%AF-i-a-prognostic-marker-for-patients-with-head-and-neck-squamous-cell-carcinomas
#8
S Chawla, L A Loevner, S G Kim, W-T Hwang, S Wang, G Verma, S Mohan, V LiVolsi, H Quon, H Poptani
BACKGROUND AND PURPOSE: Shutter-speed model analysis of dynamic contrast-enhanced MR imaging allows estimation of mean intracellular water molecule lifetime (a measure of cellular energy metabolism) and volume transfer constant (a measure of hemodynamics). The purpose of this study was to investigate the prognostic utility of pretreatment mean intracellular water molecule lifetime and volume transfer constant in predicting overall survival in patients with squamous cell carcinomas of the head and neck and to stratify p16-positive patients based upon survival outcome...
November 16, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/29106751/fast-na-k-pump-driven-steady-state-transcytolemmal-water-exchange-in-neuronal-tissue-a-study-of-rat-brain-cortical-cultures
#9
Ruiliang Bai, Charles S Springer, Dietmar Plenz, Peter J Basser
PURPOSE: Water homeostasis and transport play important roles in brain function (e.g., ion homeostasis, neuronal excitability, cell volume regulation, etc.). However, specific mechanisms of water transport across cell membranes in neuronal tissue have not been completely elaborated. METHODS: The kinetics of transcytolemmal water exchange were measured in neuronal tissue using simultaneous, real-time fluorescence and nuclear magnetic resonance (NMR) measurements of perfused, active brain organotypic cortical cultures...
November 6, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29077582/neuroimaging-in-the-diagnosis-of-chronic-traumatic-encephalopathy-a-systematic-review
#10
Philip Sparks, Tim Lawrence, Stephan Hinze
OBJECTIVE: Chronic traumatic encephalopathy (CTE) is a neurodegenerative tauopathy associated with repeated subconcussive and concussive head injury. Clinical features include cognitive, behavioral, mood, and motor impairments. Definitive diagnosis is only possible at postmortem. Here, the utility of neuroimaging in the diagnosis of CTE is evaluated by systematically reviewing recent evidence for changes in neuroimaging biomarkers in suspected cases of CTE compared with controls. DATA SOURCES: Providing an update on a previous systematic review of articles published until December 2014, we searched for articles published between December 2014 and July 2016...
October 25, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/29026086/picosecond-orientational-dynamics-of-water-in-living-cells
#11
Martijn Tros, Linli Zheng, Johannes Hunger, Mischa Bonn, Daniel Bonn, Gertien J Smits, Sander Woutersen
Cells are extremely crowded, and a central question in biology is how this affects the intracellular water. Here, we use ultrafast vibrational spectroscopy and dielectric-relaxation spectroscopy to observe the random orientational motion of water molecules inside living cells of three prototypical organisms: Escherichia coli, Saccharomyces cerevisiae (yeast), and spores of Bacillus subtilis. In all three organisms, most of the intracellular water exhibits the same random orientational motion as neat water (characteristic time constants ~9 and ~2 ps for the first-order and second-order orientational correlation functions), whereas a smaller fraction exhibits slower orientational dynamics...
October 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29018100/randomized-crossover-trial-of-blood-volume-monitoring-guided-ultrafiltration-biofeedback-to-reduce-intradialytic-hypotensive-episodes-with-hemodialysis
#12
Kelvin C W Leung, Robert R Quinn, Pietro Ravani, Henry Duff, Jennifer M MacRae
BACKGROUND AND OBJECTIVES: Intradialytic hypotension (IDH) is associated with morbidity. The effect of blood volume-guided ultrafiltration biofeedback, which automatically adjusts fluid removal rate on the basis of blood volume parameters, on the reduction of IDH was tested in a randomized crossover trial. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We performed a 22-week, single blind, randomized crossover trial in patients receiving maintenance hemodialysis who had >30% of sessions complicated by symptomatic IDH in five centers in Calgary, Alberta, Canada...
November 7, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28979785/estimated-dietary-sodium-intake-in-haemodialysis-patients-using-food-frequency-questionnaires
#13
Anastasia Gkza, Andrew Davenport
BACKGROUND: In clinical practice, dietary sodium assessment requires reliable and rapid screening tools. We wished to evaluate the usefulness of food frequency questionnaires (FFQ) in estimating dietary sodium intakes in haemodialysis patients. METHODS: We used the Derby Salt Questionnaire (DSQ), and Scored Sodium Questionnaire (SSQ) to estimate sodium intake. Body composition was determined by bioimpedance. RESULTS: In total, 139 haemodialysis patients (95 men) completed the FFQs, with mean ± standard deviation age 67 ± 15 years...
October 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28971467/bioimpedance-measurements-in-adolescents-with-polycystic-ovary-syndrome-a-pilot-study
#14
Styliani A Geronikolou, Flora Bacopoulou, Dennis Cokkinos
Limited data are available on the body composition of adolescent women with polycystic ovary syndrome (PCOS). The aim of this study was to examine differences in body composition indices of metabolism, homeostasis and inflammation, between Greek adolescent females suffering from PCOS and age- and body mass index (BMI)-matched non-PCOS controls. Thirteen PCOS patients and nine non-PCOS controls, aged 13-24 years participated in this cross-sectional study. Study participants underwent assessment by a novel dual frequency bioimpedance device (BIA-ACC)...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28830412/body-composition-and-personality-traits-in-so-yang-type-males
#15
Jiwon Yoon, Jiho Nam, Chae Hun Leem, Jong Yeol Kim
BACKGROUND: The purpose of the present study was to examine the body composition of So-Yang type males according to Sasang constitutional medicine, which is popular in Korea. Different Sasang constitutional types are associated with different muscle distributions, body shapes, and disease susceptibilities. We used the Sasang Personality Questionnaire (SPQ) as a measure of the temperament of each Sasang type. METHODS: In total, 953 subjects aged over 20 years were recruited in Korea...
August 22, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28820993/changing-profiles-of-bound-water-content-and-distribution-in-the-activated-sludge-treatment-by-nacl-addition-and-ph-modification
#16
Dong-Qin He, Ying-Jie Zhang, Chuan-Shu He, Han-Qing Yu
Currently the dewatering of activated sludge from wastewater treatment plants is a problem not well solved. Extracellular polymeric substances (EPS), including loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS), are highly hydrated biopolymers and play important roles in sludge dewatering. In the present work, two types of treatments, i.e., salt addition and pH modification, were proposed to evaluate the effects of bound water content and its distribution on the dewatering performance of activated sludge...
November 2017: Chemosphere
https://www.readbyqxmd.com/read/28757053/cryopreservation-of-indian-red-jungle-fowl-gallus-gallus-murghi-semen-with-polyvinylpyrrolidone
#17
Bushra Allah Rakha, Muhammad Sajjad Ansari, Shamim Akhter, Zartasha Zafar, Iftikhar Hussain, Julian Santiago-Moreno, Elisabeth Blesbois
The Indian red jungle fowl is a sub-species of the genus Gallus native to South Asia; facing high risk of extinction in its native habitat. During cryopreservation, permeable cryoprotectants like glycerol are usually employed and we previously showed encouraging results with 20% glycerol. Because bird spermatozoa contain very little intracellular water, the possibility of replacing an internal cryoprotectant by an external one is opened. In the present study, we tested the replacement of internal cryoprotectant glycerol by the external cryoprotectant Polyvinylpyrrolidone (PVP)...
July 27, 2017: Cryobiology
https://www.readbyqxmd.com/read/28755131/increase-in-echo-intensity-and-extracellular-to-intracellular-water-ratio-is-independently-associated-with-muscle-weakness-in-elderly-women
#18
Masashi Taniguchi, Yosuke Yamada, Yoshihiro Fukumoto, Shinichiro Sawano, Seigo Minami, Tome Ikezoe, Yuya Watanabe, Misaka Kimura, Noriaki Ichihashi
PURPOSE: The changes in muscle composition and its heterogeneity during aging are associated with muscle weakness in elderly persons independent of decreases in muscle mass or muscle thickness (MT). Both the assessment of echo intensity (EI) with ultrasound imaging and the evaluation of the extracellular water/intracellular water (ECW/ICW) ratio with segmental bioelectrical impedance spectroscopy (BIS) are non-invasive and convenient methods and seem valuable for muscle quality determination...
October 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28701677/mechanisms-of-diuresis-for-acute-decompensated-heart-failure-by-tolvaptan
#19
RANDOMIZED CONTROLLED TRIAL
Hidetsugu Nomoto, Yasuhiro Satoh, Mayu Kamiyama, Kento Yabe, Mayumi Masumura, Atsushi Sakakibara, Shu Yamashita, Masahito Suzuki, Tomoyo Sugiyama, Tetsuo Oumi, Masakazu Ohno, Yoshihide Takahashi, Mitsuaki Isobe
Tolvaptan, a vasopressin type 2 receptor antagonist, does not affect kidney circulation or cause worsening of renal function (WRF) in patients with acute decompensated heart failure (ADHF). Bioelectrical impedance analysis (BIA) can be used to evaluate intravascular volume by calculating the ratio of extracellular water (ECW) to intracellular water (ICW). There have been no reports examining the mechanisms of tolvaptan-induced diuresis using BIA. We investigated whether tolvaptan decreases excess volume while maintaining intravascular volume in ADHF patients...
August 3, 2017: International Heart Journal
https://www.readbyqxmd.com/read/28699923/the-effects-of-moderate-versus-high-load-resistance-training-on-muscle-growth-body-composition-and-performance-in-collegiate-women
#20
Jason M Cholewa, Fabricio E Rossi, Christopher MacDonald, Amy Hewins, Samantha Gallo, Ashley Micenski, Layne Norton, Bill I Campbell
Twenty young women (20.3+1.5 years, 164+6 cm, 68.7+13.8 kg) without prior structured resistance training experience were recruited for this study. Body composition (BodPod), compartmental water (Bioelectrical Impedance), 7-site skinfold, and arm and thigh CSA were assessed pre- and post- 8 week training. Performance testing consisted of vertical jump, 3 kg chest pass initial velocity, squat 1RM and overhead press 1RM. Following 2 weeks of familiarization training, subjects were matched for body composition and relative squat strength, and randomly assigned to either a high- (HL: n=10; 4 sets of 5-7 repetitions) or moderate-load (ML: n=10; 2 sets of 10-14 repetitions) group that completed 6-7 exercises per day performed to momentary muscular failure...
June 2, 2017: Journal of Strength and Conditioning Research
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