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Anthocyanin, transcription

Haitao Shi, Guoyin Liu, Yunxie Wei, Zhulong Chan
The accumulation of flavonoids is activated by various abiotic stresses, and the induction of reactive oxygen species (ROS) especially hydrogen peroxide (H2 O2 ) is a general response to abiotic stress in plants. However, the direct link between flavonoids and H2 O2 and underlying mechanism remain elusive. In this study, we found that the concentrations of anthocyanin and flavonoids were significantly induced by H2 O2 treatment. Furthermore, we found that the transcript level of ZINC FINGER of ARABIDOPSIS THALIANA 6 (ZAT6) was significantly activated after exogenous H2 O2 treatment, and modulation of AtZAT6 expression positively affected the concentrations of both anthocyanin and total flavonoids...
April 19, 2018: Plant Molecular Biology
Dong Liang, Yanqiu Shen, Zhiyou Ni, Qin Wang, Zhi Lei, Nengqin Xu, Qunxian Deng, Lijin Lin, Jin Wang, Xiulan Lv, Hui Xia
Melatonin, a multiple signal molecule, plays important roles in delaying senescence during the development of plants. Because few species have been studied for the effect of exogenous melatonin on anti-aging, the plausible mechanism of melatonin of anti-aging effects on other plant species has remained largely unknown. In the present study, the effects of exogenous melatonin on leaf senescence in kiwifruit were examined during natural aging after melatonin (200 μM) or water (Control) pretreatment. The decreased membrane damage and lower hydrogen peroxide (H2 O2 ) content due to the enhanced scavenging activity of antioxidant enzymes peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) demonstrated that melatonin effectively delayed the aging of kiwifruit leaves...
2018: Frontiers in Plant Science
Chunhua Zhang, Ruchao Feng, Ruijuan Ma, Zhijun Shen, Zhixiang Cai, Zhizhong Song, Bin Peng, Mingliang Yu
The basic helix-loop-helix (bHLH) transcription factor family is a superfamily found in all eukaryotes that plays important roles in regulating growth and development. Over the past several decades, many bHLH superfamily genes have been identified and characterized in herbaceous and woody plants. However, the genes belonging to the bHLH superfamily in peach (Prunus persica) have not yet been comprehensively identified and characterized. Here, we identified 95 members of the bHLH superfamily in the peach genome, and these genes were classified into 19 subfamilies based on a phylogenetic comparison with bHLH proteins from Arabidopsis...
2018: PloS One
Simon Bulman, Franziska Richter, Sabine Marschollek, Freia Benade, Sabine Jülke, Jutta Ludwig-Müller
The plant pathogenic protist Plasmodiophora brassicae causes the clubroot disease of Brassicaceae. The biotrophic organism has the capacity to downregulate plant defence responses. The previously characterised P. brassicae PbBSMT methyltransferase has substrate specificity for salicylic, benzoic and anthranilic acids. We therefore propose a role for the methylation of SA in attenuating the plant's defence response in infected roots as a novel strategy for intracellular parasitism. To further elucidate the function of PbBSMT, we overexpressed the gene under the control of an inducible promoter in Arabidopsis thaliana and performed physiological, molecular and phytopathological analyses with these transgenic plants under control and induced conditions in comparison to wild type...
April 1, 2018: Plant Biology
Muhammad Anwar, Guiqing Wang, Jiacheng Wu, Saquib Waheed, Andrew C Allan, Lihui Zeng
R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factor NtMYB2 was identified from Chinese narcissus ( Narcissus tazetta L. var. Chinensis Roem) and functionally characterized. NtMYB2 belongs to subgroup 4 of the R2R3 MYB transcription factor family that are related to repressor MYBs involved in the regulation of anthocyanin and flavonoids. Transient expression confirmed that NtMYB2 strongly reduced the red pigmentation induced by MYB- anthocyanin activators in agro-infiltrated tobacco leaves...
March 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiaolan Jiang, Keyi Huang, Guangshun Zheng, Hua Hou, Peiqiang Wang, Han Jiang, Xuecheng Zhao, Mingzhuo Li, Shuxiang Zhang, Yajun Liu, Liping Gao, Lei Zhao, Tao Xia
Tea is one of the most widely consumed nonalcoholic beverages worldwide. Polyphenols are nutritional compounds present in the leaves of tea plants. Although numerous genes are functionally characterized to encode enzymes that catalyze the formation of diverse polyphenolic metabolites, transcriptional regulation of those different pathways such as late steps of the proanthcoyanidin (PA) pathway remains unclear. In this study, using different tea transcriptome databases, we screened at least 140 R2R3-MYB transcription factors (TFs) and grouped them according to the basic function domains of the R2R3 MYB TF superfamily...
May 2018: Plant Science: An International Journal of Experimental Plant Biology
Chao-Chao Liu, Cheng Chi, Li-Juan Jin, Jianhua Zhu, Jing-Quan Yu, Yan-Hong Zhou
The production of anthocyanin is regulated by light and corresponding photoreceptors. In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.). These responses are the result of changes in mRNA and the protein levels of SlHY5, a transcription factor. In vitro and in vivo experiments using EMSA and ChIP-qPCR assays revealed that SlHY5 could directly recognize and bind to the G-box and ACE motifs in the promoters of anthocyanin biosynthesis genes, such as CHS1, CHS2 and DFR...
March 22, 2018: Plant, Cell & Environment
Wenhui Jiang, Tianxiang Liu, Wenzhi Nan, Diddugodage Chamila Jeewani, Yanlu Niu, Chunlian Li, Yong Wang, Xue Shi, Cong Wang, Jiahuan Wang, Yang Li, Xin Gao, Zhonghua Wang
The purple pericarp of bread wheat (Triticum aestivum L.) is a useful source of dietary anthocyanins. Previous mapping results indicated that the purple pericarp is controlled by two complementary genes located on chromosomes 7D and 2A. However, the related genes and their effects on regulating the purple pericarp are unknown. In this study, two transcription factors were characterised as anthocyanin activators in purple pericarp: TaPpm1 (purple pericarp-MYB 1) and TaPpb1 (purple pericarp-bHLH 1). Three nonfunctional variants were detected in the coding sequence of TaPpm1 from non-purple seed lines, in which the function of TaPpm1 was destroyed by insertion-caused frame shift or truncated peptides...
March 17, 2018: Journal of Experimental Botany
Olesya Yu Shoeva
The wheat TaMyc1 gene encodes for transcriptional factor (TF) with bHLH domain. The gene is expressed in purple wheat grains and activates transcription of the anthocyanin biosynthesis structural genes. To reveal the features of TaMyc1 regulation in wheat pericarp transcription start sites (TSS) were identified by 5' RACE mean and translation efficiency was predicted by in silico methods. Three alternative transcript variants of TaMyc1 differing by 5' leader sequence only were identified in purple pericarp...
March 19, 2018: Molecular Biology Reports
Carlos Daniel Crocco, Gabriel Gomez-Ocampo, Anita Mantese, Edmundo L Ploschuk, Javier Francisco Botto
B-box (BBX) proteins are zinc-finger transcription factors containing one or two B-box motifs. BBX proteins act as key factors in the networks regulating growth and development. The relevance of BBX21 to light and abscisic acid signaling in seedling development is well stablished, however its importance in adult plant development and agronomic species is poorly understood. Therefore, we studied the effect of heterologous expression of Arabidopsis thaliana BBX21 in potato (Solanum tuberosum) var. Spunta. Three independent AtBBX21-expressing lines and the wild-type control were cultivated under sunlight and at controlled temperatures in a greenhouse...
March 19, 2018: Plant Physiology
Jing Li, Yong-Jun He, Lu Zhou, Yang Liu, Mingmin Jiang, Li Ren, Huoying Chen
BACKGROUND: The anthocyanins are highly enriched in eggplants (Solanum melongena L.) with purple peel. However, our previous study showed that anthocyanins biosynthesis in eggplant cultivar 'Lanshan Hexian' was completely regulated by light and color becomes evident at most 2 days after exposure to light. In the present investigation, transcriptome study was made to explore the underlying molecular mechanisms of light-induced anthocyanin biosynthesis in eggplant (Solanum melongena L...
March 20, 2018: BMC Genomics
Jian Liang, Junxian He
Nitrogen (N) is a major nutrient of plants but often a limiting factor for plant growth and crop yield. To adapt to N deficiency, plants have evolved adaptive responses including accumulation of anthocyanins. However, it is still unclear whether the accumulated anthocyanins are part of the components of plant tolerance under low N stress. Here, we demonstrate that low N-induced anthocyanins contribute substantially to the low N tolerance of Arabidopsis thaliana. pap1-1, a mutant defective in MYB75 (PAP1), a MYB-type transcription factor that positively regulates anthocyanin biosynthesis in Arabidopsis, was found to have significantly decreased survival rate to low N stress compared to its wild-type plants...
March 16, 2018: Biochemical and Biophysical Research Communications
Ruiyan Tao, Songling Bai, Junbei Ni, Qinsong Yang, Yuan Zhao, Yuanwen Teng
A conserved blue light sensing and transduction pathway contributes to blue light-induced anthocyanin accumulation in the peel of red pear. Peel color is an economically important characteristic that influences the appearance quality of red pear, whose red color is due to anthocyanin accumulation. The process of coloration in the fruit peel is strongly influenced by light. However, how light quality influences color development remains unclear. In this study, we analyzed the effects of different light qualities on color development in the red pear 'Red Zaosu', a mutant of the hybrid cultivar 'Zaosu' of Pyrus pyrifolia and P...
March 15, 2018: Planta
Liyu Shi, Shifeng Cao, Xin Chen, Wei Chen, Yonghua Zheng, Zhenfeng Yang
Proanthocyanidins (PAs) are distributed widely in Chinese bayberry fruit and have been associated with human health benefits, but molecular and biochemical characterization of PA biosynthesis remains unclear. Here, two genes encoding key PA biosynthetic enzymes, anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) were isolated in bayberry fruit. MrANR was highly expressed at the early stage of fruit development when soluble PAs accumulated at high levels. Meanwhile, the transcript abundance of both MrANR and MrLAR observed at the late stage was paralleled with the high amounts of insoluble PAs...
2018: Frontiers in Plant Science
Huifeng Luo, Cheng Dai, Yongping Li, Jia Feng, Zhongchi Liu, Chunying Kang
The red color of the foliage and fruit in strawberry comes from anthocyanins stored in the vacuole. However, how anthocyanin accumulation is regulated in strawberry is still unclear. A reduced anthocyanin in petioles (rap) mutant was identified in an ENU mutagenized population of YW5AF7, a white-fruited variety of the wild strawberry Fragaria vesca. The causative mutation was identified to be a premature stop codon in a glutathione S-transferase (GST) gene. In addition to the foliage coloration, RAP also mediates fruit pigmentation and acts downstream of the fruit-specific transcription factor FvMYB10...
March 10, 2018: Journal of Experimental Botany
Hee Kyoung Joo, Sunga Choi, Yu Ran Lee, Eun Ok Lee, Myoung Soo Park, Kyu Been Park, Cuk-Seong Kim, Yong Pyo Lim, Jong-Tae Park, Byeong Hwa Jeon
Anthocyanins, the most prevalent flavonoids in red/purple fruits and vegetables, are known to improve immune responses and reduce chronic disease risks. In this study, the anti-inflammatory activities of an anthocyanin-rich extract from red Chinese cabbage (ArCC) were shown based on its inhibitory effects in cultured endothelial cells and hyperlipidemic apolipoprotein E -deficient mice. ArCC treatment suppressed monocyte adhesion to tumor necrosis factor-α-stimulated endothelial cells. This was validated by ArCC's ability to downregulate the expression and transcription of endothelial adhesion molecules, determined by immunoblot and luciferase promoter assays, respectively...
March 12, 2018: International Journal of Molecular Sciences
Jiaxuan Guo, Shufang Wang, XiaoYang Yu, Rui Dong, Yuzhong Li, Xurong Mei, Yuanyue Shen
Polyamines (PAs) participate in many plant growth and developmental processes, including fruit ripening. However, it is not clear whether PAs play a role in the ripening of strawberry (Fragaria ananassa), a model non-climacteric plant. Here, we found that the content of the PA spermine (Spm) increased more sharply after the onset of fruit coloration than did that of the PAs putrescine (Put) or spermidine (Spd). Spm dominance in ripe fruit resulted from abundant transcripts of a strawberry S-adenosyl-L-methionine decarboxylase gene (FaSAMDC), which encodes an enzyme that generates a residue needed for PA biosynthesis...
March 9, 2018: Plant Physiology
Fang Wang, Yu Xiu Dong, Xiao Zhen Tang, Tian Li Tu, Bin Zhao, Na Sui, Daolin Fu, Xian Sheng Zhang
To study mechanism of anthocyanin biosynthesis regulation, we examined light-regulated gene expression involved in anthocyanin biosynthesis in purple grain of wheat. Ten kinds of anthocyanins were identified from a purple-grain wheat cultivar by HPLC ESI-MS/MS analysis. The libraries constructed from total RNA of purple grain under light (L) or dark (D) conditions for 15 and 20 days were sequenced. In total, 1,874 differentially expressed genes (DEGs) in L20 vs. L15, 1,432 DEGs in D20 vs. D15, 862 DEGs in D15 vs...
March 9, 2018: Journal of Agricultural and Food Chemistry
Dóra Faragó, László Sass, Ildikó Valkai, Norbert Andrási, László Szabados
Plant size, shape and color are important parameters of plants, which have traditionally been measured by destructive and time-consuming methods. Non-destructive image analysis is an increasingly popular technology to characterize plant development in time. High throughput automatic phenotyping platforms can simultaneously analyze multiple morphological and physiological parameters of hundreds or thousands of plants. Such platforms are, however, expensive and are not affordable for many laboratories. Moreover, determination of basic parameters is sufficient for most studies...
2018: Frontiers in Plant Science
Yuyan Sun, Jinglei Wang, Yang Qiu, Tongjin Liu, Jiangping Song, Xixiang Li
Radish is an economically important vegetable crop belonging to the family Brassicaceae. The high anthocyanin content of the 'Xinlimei' radish roots has been associated with diverse health benefits. However, there is a lack of transcript-level information regarding anthocyanin biosynthesis. In the present study, the 'Xinlimei' radish root transcriptome was analyzed by RNA sequencing at five developmental stages. A total of 222,384,034 clean reads were obtained and 32,253 unigenes were annotated. Expression profiles revealed 10,890 differentially expressed genes (DEGs) among the five analyzed libraries...
March 5, 2018: Gene
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