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https://www.readbyqxmd.com/read/28340485/an-approach-for-liposome-immobilization-using-sterically-stabilized-micelles-ssms-as-a-precursor-for-bio-layer-interferometry-based-interaction-studies
#1
Jakob Wallner, Gabriele Lhota, Markus Schosserer, Karola Vorauer-Uhl
Non-fluidic bio-layer interferometry (BLI) has rapidly become a standard tool for monitoring almost all biomolecular interactions in a label-free, real-time and high-throughput manner. High-efficiency screening methods which measure the kinetics of liposomes with a variety of compounds require the immobilization of liposomes. In this work, a method is described for immobilizing liposomes for interaction studies, based on the biophysical principles of this biosensor platform. The immobilization approach includes the loading of DSPE-PEG(2000)-biotin containing sterically stabilized micelles (SSMs) which are restructured in a buffer change step, resulting in an accessible substrate for liposome immobilization...
March 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28340435/optimization-of-hydrogen-dispersion-in-thermophilic-up-flow-reactors-for-ex-situ-biogas-upgrading
#2
Ilaria Bassani, Panagiotis G Kougias, Laura Treu, Hugo Porté, Stefano Campanaro, Irini Angelidaki
This study evaluates the efficiency of four novel up-flow reactors for ex situ biogas upgrading converting externally provided CO2 and H2 to CH4, via hydrogenotrophic methanogenesis. The gases were injected through stainless steel diffusers combined with alumina ceramic sponge or through alumina ceramic membranes. Pore size, input gas loading and gas recirculation flow rate were modulated to optimize gas-liquid mass transfer, and thus methanation efficiency. Results showed that larger pore size diffusion devices achieved the best kinetics and output-gas quality converting all the injected H2 and CO2, up to 3...
March 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340433/feasibility-of-carbon-dioxide-sequestration-by-spongiochloris-sp-microalgae-during-petroleum-wastewater-treatment-in-airlift-bioreactor
#3
Abdeldjalil Abid, Faten Saidane, Moktar Hamdi
The aim of this work was to study the ability of using Hydrocabonoclastic native microbial and Spongiochloris sp microalgae in airlift bioreactors couples in order to restore hydrocarbons wastewater and develop the capacity of natural systems to reduce greenhouse effect through maximal control of CO2 gas emission in atmosphere. The kinetic parameters of CO2 gas fixation level and conversion it into biological material by microalgae as the biodegradation process effect in hydrocarbon have been evaluated. The result present that maximum specific growth rate μmax of Spongiochloris sp was (0...
March 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28340419/photolytic-degradation-of-the-%C3%AE-blocker-nebivolol-in-aqueous-solution
#4
Alaa Salma, Holger V Lutze, Torsten C Schmidt, Jochen Tuerk
Nebivolol (NEB) is one of the top-sold prescription drugs belonging to the third generation of beta-blockers. However, so far, occurrence data in the environment are lacking. Within this study NEB has been found for the first time in effluent samples of wastewater treatment plants in Germany with an average concentration of 13 ng L(-1). Its photodegradation behavior in the environment and in technical processes is largely unknown. To fill this gap, three different UV treatment procedures (UV-C at 254 nm, UV-B at 312 nm and UV-A at 365 nm) were investigated in three different matrices: pure water, pure water in presence of the hydroxyl radical (OH) scavenger tert...
March 13, 2017: Water Research
https://www.readbyqxmd.com/read/28340418/enantioselective-reductive-transformation-of-climbazole-a-concept-towards-quantitative-biodegradation-assessment-in-anaerobic-biological-treatment-processes
#5
Monica Brienza, Serge Chiron
An efficient chiral method-based using liquid chromatography-high resolution-mass spectrometry analytical method has been validated for the determination of climbazole (CBZ) enantiomers in wastewater and sludge with quantification limits below the 1 ng/L and 2 ng/g range, respectively. On the basis of this newly developed analytical method, the stereochemistry of CBZ was investigated over time in sludge biotic and sterile batch experiments under anoxic dark and light conditions and during wastewater biological treatment by subsurface flow constructed wetlands...
March 21, 2017: Water Research
https://www.readbyqxmd.com/read/28340410/development-of-fusogenic-glass-surfaces-that-impart-spatiotemporal-control-over-macrophage-fusion-direct-visualization-of-multinucleated-giant-cell-formation
#6
James J Faust, Wayne Christenson, Kyle Doudrick, Robert Ros, Tatiana P Ugarova
Implantation of synthetic material, including vascular grafts, pacemakers, etc. results in the foreign body reaction and the formation of multinucleated giant cells (MGCs) at the exterior surface of the implant. Despite the long-standing premise that fusion of mononucleated macrophages results in the formation of MGCs, to date, no published study has shown fusion in context with living specimens. This is due to the fact that optical-quality glass, which is required for the majority of live imaging techniques, does not promote macrophage fusion...
March 2, 2017: Biomaterials
https://www.readbyqxmd.com/read/28340407/transmission-of-persistent-ionizing-radiation-induced-foci-through-cell-division-in-human-primary-cells
#7
Aurelie Vaurijoux, Pascale Voisin, Amelie Freneau, Joan Francesc Barquinero, Gaetan Gruel
Unrepaired DNA double-strand breaks (DSBs) induced by ionizing radiation are associated with lethal effects and genomic instability. After the initial breaks and chromatin destabilization, a set of post-translational modifications of histones occurs, including phosphorylation of serine 139 of histone H2AX (γH2AX), which leads to the formation of ionizing radiation-induced foci (IRIF). DSB repair results in the disappearance of most IRIF within hours after exposure, although some remain 24h after irradiation...
March 10, 2017: Mutation Research
https://www.readbyqxmd.com/read/28340369/degradation-of-diclofenac-by-uv-activated-persulfate-process-kinetic-studies-degradation-pathways-and-toxicity-assessments
#8
Xian Lu, Yisheng Shao, Naiyun Gao, Juxiang Chen, Yansen Zhang, Huiming Xiang, Youluo Guo
Diclofenac (DCF) is the frequently detected non-steroidal pharmaceuticals in the aquatic environment. In this study, the degradation of DCF was evaluated by UV-254nm activated persulfate (UV/PS). The degradation of DCF followed the pseudo first-order kinetics pattern. The degradation rate constant (kobs) was accelerated by UV/PS compared to UV alone and PS alone. Increasing the initial PS dosage or solution pH significantly enhanced the degradation efficiency. Presence of various natural water constituents had different effects on DCF degradation, with an enhancement or inhibition in the presence of inorganic anions (HCO3(-) or Cl(-)) and a significant inhibition in the presence of NOM...
March 21, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28340336/a-dna-based-t-cell-receptor-reveals-a-role-for-receptor-clustering-in-ligand-discrimination
#9
Marcus J Taylor, Kabir Husain, Zev J Gartner, Satyajit Mayor, Ronald D Vale
A T cell mounts an immune response by measuring the binding strength of its T cell receptor (TCR) for peptide-loaded MHCs (pMHC) on an antigen-presenting cell. How T cells convert the lifetime of the extracellular TCR-pMHC interaction into an intracellular signal remains unknown. Here, we developed a synthetic signaling system in which the extracellular domains of the TCR and pMHC were replaced with short hybridizing strands of DNA. Remarkably, T cells can discriminate between DNA ligands differing by a single base pair...
March 23, 2017: Cell
https://www.readbyqxmd.com/read/28340192/prediction-equation-for-calculating-residual-kidney-urea-clearance-using-urine-collections-for-different-hemodialysis-treatment-frequencies-and-interdialytic-intervals
#10
Yoshitsugu Obi, Kamyar Kalantar-Zadeh, Elani Streja, John T Daugirdas
Background.: The purpose of the study was to explore the precision of an equation designed to estimate residual kidney urea clearance (K RU ) from interdialytic urine collection data and pre-hemodialysis (HD) serum urea nitrogen (SUN) in different hemodialysis treatment schedules. Methods.: The generalizability of the proposed equation was tested in 32 731 HD treatments where urine was collected prior to a dialysis session, mostly for 24 h but sometimes longer, in patients being dialyzed 1-4 times/week...
March 15, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28340139/assessment-of-acute-kidney-injury-in-rhabdomyolytic-mice-by-transcutaneous-measurement-of-sinistrin-excretion
#11
Aldo Moggio, Stefania Geraci, Alberto Boido, Carsten Sticht, Norbert Gretz, Benedetta Bussolati
Background: . Early and accurate assessment of renal function is required for the successful detection and treatment of acute kidney injury (AKI). However, only retention parameters such as plasma urea and creatinine, and the indirect estimation of glomerular filtration rate are commonly available. Methods.: Here, we measured the kinetics of plasma fluorescein isothiocyanate (FITC)-sinistrin excretion to detect alterations of renal function over time in a murine model of rhabdomyolysis-induced AKI...
February 27, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28340058/predictive-modeling-for-monitoring-egg-freshness-during-variable-temperature-storage-conditions
#12
S M Yimenu, J Y Kim, J Koo, B S Kim
The overall aim of this research was to develop egg freshness prediction models in terms of selected quality indices. Six experiments (4 constant temperatures and 2 variable temperatures) were carried out on hen eggs for a total period of 10, 21, 26, 13, and 105 d at storage temperatures of 30, 20, 20 to 10, 30 to 10, and 5 and 10°C, to observe trends in the relative weight loss (RWL), Haugh unit (HU), yolk index (YI), albumin index (AI), yolk pH, and albumin pH. The results showed that there was an increasing trend in the RWL and a decreasing trend in the YI, AI, and HU for all temperature conditions...
March 2, 2017: Poultry Science
https://www.readbyqxmd.com/read/28340027/influence-of-long-term-in-vivo-exposure-debris-accumulation-and-archwire-material-on-friction-force-among-different-types-of-brackets-and-archwires-couples
#13
Uroš Mezeg, Jasmina Primožic
Objective: The aim was to assess the influence of long-term in vivo exposure, debris accumulation and archwire material on static and kinetic friction force among different types of brackets and archwires couples. Material and Methods: Friction testing was performed among four lower incisors' brackets, conventional and self-ligating (SL), coupled with either nickel-titanium or stainless steel archwires, as-received and in vivo exposed in 18 subjects. The friction testing was performed for a sliding distance of 14 mm at a speed of 10 mm/min, with a starting force of 0...
February 28, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28339682/the-effects-of-interleukin-2-on-immune-response-regulation
#14
Ryan S Waters, Justin S A Perry, SunPil Han, Bibiana Bielekova, Tomas Gedeon
The immune system has many adaptive and dynamic components that are regulated to ensure appropriate, precise and rapid response to a foreign pathogen. A delayed or inadequate immune response can lead to prolonged disease, while an excessive or under-regulated response can lead to autoimmunity. The cytokine, interleukin-2 (IL-2) and its receptor IL-2R play an important role in maintaining this balance.The IL-2 receptor transduces pSTAT5 signal through both the intermediate and high affinity receptors, which differ from each other by the presence of CD25 chain in IL-2 receptor...
February 20, 2017: Mathematical Medicine and Biology: a Journal of the IMA
https://www.readbyqxmd.com/read/28339479/mouse-newborn-cells-allow-highly-productive-mouse-cytomegalovirus-replication-constituting-a-novel-convenient-primary-cell-culture-system
#15
Vu Thuy Khanh Le-Trilling, Mirko Trilling
Mammalian cell culture is indispensable for most aspects of current biomedical research. Immortalized cell lines are very convenient, but transforming principles (e.g. oncogenic viruses or their oncogenes) can heavily influence the experimental outcome. Primary cells do not share this apparent disadvantage but are more laborious to generate. Certain viruses (e.g. mouse cytomegalovirus) do not replicate efficiently in most transformed cell lines. In the past, such viruses have been routinely propagated on primary mouse embryonic fibroblasts (MEF) established around day 17 (d17) of gestation...
2017: PloS One
https://www.readbyqxmd.com/read/28339350/the-effect-of-initial-knee-angle-on-concentric-only-squat-jump-performance
#16
Lachlan J Mitchell, Christos K Argus, Kristie-Lee Taylor, Jeremy M Sheppard, Dale W Chapman
PURPOSE: There is uncertainty as to which knee angle during a squat jump (SJ) produces maximal jump performance. Importantly, understanding this information will aid in determining appropriate ratios for assessment and monitoring of the explosive characteristics of athletes. METHOD: This study compared SJ performance across different knee angles-90º, 100º, 110º, 120º, 130º, and a self-selected depth-for jump height and other kinetic characteristics. For comparison between SJ and an unconstrained dynamic movement, participants also performed a countermovement jump from a self-selected depth...
March 24, 2017: Research Quarterly for Exercise and Sport
https://www.readbyqxmd.com/read/28339283/development-optimization-and-implementation-of-a-centralized-metabolic-soft-spot-assay
#17
Anthony A Paiva, Cheryl Klakouski, Shu Li, Benjamin M Johnson, Yue-Zhong Shu, Jonathan Josephs, Tatyana Zvyaga, Ismael Zamora, Wilson Z Shou
AIM: High clearance is a commonly encountered issue in drug discovery. Here we present a centralized metabolic soft spot identification assay with adequate capacity and turnaround time to support the metabolic optimization needs of an entire discovery organization. METHODOLOGY: An integrated quan/qual approach utilizing both an orthogonal sample-pooling methodology and software-assisted structure elucidation was developed to enable the assay. Major metabolic soft spots in liver microsomes (rodent and human) were generated in a batch mode, along with kinetics of parent disappearance and metabolite formation, typically within 1 week of incubation...
March 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28339277/turbulence-nonlinearities-shed-light-on-geometric-asymmetry-in-tokamak-confinement-transitions
#18
I Cziegler, A E Hubbard, J W Hughes, J L Terry, G R Tynan
A comprehensive study of fully frequency-resolved nonlinear kinetic energy transfer has been performed for the first time in a diverted tokamak, providing new insight into the parametric dependences of edge turbulence transitions. Measurements using gas puff imaging in the turbulent L-mode state illuminate the source of the long known but as yet unexplained "favorable-unfavorable" geometric asymmetry of the power threshold for transition to the turbulence-suppressed H mode. Results from the recently discovered I mode point to a competition between zonal flow (ZF) and geodesic-acoustic modes (GAM) for turbulent energy, while showing new evidence that the I-to-H transition is still dominated by ZFs...
March 10, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28339247/thermodynamic-and-kinetic-fragility-of-freon-113-the-most-fragile-plastic-crystal
#19
A Vispa, M Romanini, M A Ramos, L C Pardo, F J Bermejo, M Hassaine, A I Krivchikov, J W Taylor, J Ll Tamarit
We present a dynamic and thermodynamic study of the orientational glass former Freon 113 (1,1,2-trichloro-1,2,2-trifluoroethane, CCl_{2}F-CClF_{2}) in order to analyze its kinetic and thermodynamic fragilities. Freon 113 displays internal molecular degrees of freedom that promote a complex energy landscape. Experimental specific heat and its microscopic origin, the vibrational density of states from inelastic neutron scattering, together with the orientational dynamics obtained by means of dielectric spectroscopy have revealed the highest fragility value, both thermodynamic and kinetic, found for this orientational glass former...
March 10, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28339231/chiral-dark-sector
#20
Raymond T Co, Keisuke Harigaya, Yasunori Nomura
We present a simple and natural dark sector model in which dark matter particles arise as composite states of hidden strong dynamics and their stability is ensured by accidental symmetries. The model has only a few free parameters. In particular, the gauge symmetry of the model forbids the masses of dark quarks, and the confinement scale of the dynamics provides the unique mass scale of the model. The gauge group contains an Abelian symmetry U(1)_{D}, which couples the dark and standard model sectors through kinetic mixing...
March 10, 2017: Physical Review Letters
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