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https://www.readbyqxmd.com/read/27921379/fibroids-that-do-not-distort-the-uterine-cavity-and-ivf-success-rates-an-observational-study-using-extensive-matching-criteria
#1
G Christopoulos, A Vlismas, R Salim, R Islam, G Trew, S Lavery
OBJECTIVE: To assess the impact of non-cavity-distorting fibroids on in vitro fertilisation (IVF) pregnancy outcomes. DESIGN: A retrospective, matched, single-centre, cohort study was performed. SETTING: The IVF unit of a tertiary, university hospital. POPULATION: We analysed all women with non-cavity-distorting uterine fibroids undergoing IVF/intracytoplasmic sperm injection (ICSI) cycles from 1 January 2011 to 1 May 2015...
December 5, 2016: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/27921302/the-role-of-embryo-movement-in-the-development-of-the-furcula
#2
A S Pollard, S Boyd, I M McGonnell, A A Pitsillides
The pectoral girdle is a complex structure which varies in its morphology between species. A major component in birds is the furcula, which can be considered equivalent to a fusion of the paired clavicles found in many mammals, and the single interclavicle found in many reptiles. These elements are a remnant of the dermal skeleton and the only intramembranous bones in the trunk. Postnatally, the furcula plays important mechanical roles by stabilising the shoulder joint and acting as a mechanical spring during flight...
December 6, 2016: Journal of Anatomy
https://www.readbyqxmd.com/read/27921090/the-effect-of-intrauterine-human-chorionic-gonadotropin-injection-before-embryo-transfer-on-the-implantation-and-pregnancy-rate-in-infertile-patients-a-randomized-clinical-trial
#3
Razieh Dehghani Firouzabadi, Sima Janati, Mohammad Hossein Razi
BACKGROUND: Implantation is one of the essential steps for the success of assisted reproductive techniques (ART). Their success depends on three main factors: embryo quality, endometrial receptivity (ER), and synchrony between embryo and endometrium. There are various factors that regulate the complex process of implantation. In this regard, one may refer to human chorionic gonadotropin (hCG) as the most important factor. OBJECTIVE: This study aims to investigate the effect of intrauterine hCG injection before embryo transfer (ET) on pregnancy outcome in infertile couples...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27921089/effect-of-l-carnitine-on-in-vitro-developmental-rate-the-zona-pellucida-and-hatching-of-blastocysts-and-their-cell-numbers-in-mouse-embryos
#4
Nasrin Khanmohammadi, Mansoureh Movahedin, Manouchehr Safari, Hamid Reza Sameni, Behpour Yousefi, Behnaz Jafari, Sam Zarbakhsh
: L-carnitine (LC) is an antioxidant with the ability to promote the growth in vitro embryo. OBJECTIVE: The goal was to evaluate the effect of LC on some indicators of embryo development and blastocyst quality including zona pellucid (ZP) thickness, the hatching of blastocysts and their cell numbers. MATERIALS AND METHODS: Mouse embryos were randomly divided into five groups and incubated with different concentrations of LC (I; 0, II; 0.5, III; 1, IV; 2 and V; 4 mg/ml) from 2-cell to hatched blastocyst...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27921085/effects-of-autologous-platelet-rich-plasma-on-implantation-and-pregnancy-in-repeated-implantation-failure-a-pilot-study
#5
Leila Nazari, Saghar Salehpour, Sedighe Hoseini, Shahrzad Zadehmodarres, Ladan Ajori
BACKGROUND: Repeated implantation failure (RIF) is a major challenge in reproductive medicine and despite several methods that have been described for management, there is little consensus on the most effective one. OBJECTIVE: This study was conducted to evaluate the effectiveness of platelet-rich plasma in improvement of pregnancy rate in RIF patients. MATERIALS AND METHODS: Twenty women with a history of RIF who were candidates for frozen-thawed embryo transfer were recruited in this study...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27920954/drosophila-chem-mutations-disrupt-epithelial-polarity-in-drosophila-embryos
#6
José M Zamudio-Arroyo, Juan R Riesgo-Escovar
Drosophila embryogenesis has proven to be an extremely powerful system for developmental gene discovery and characterization. We isolated five new EMS-induced alleles that do not complement the l(3R)5G83 lethal line isolated in the Nüsslein-Volhard and Wieschaus screens. We have named this locus chem. Lethality of the new alleles as homozygous zygotic mutants is not completely penetrant, and they have an extended phenocritical period. Like the original allele, a fraction of mutant embryos die with cuticular defects, notably head involution and dorsal closure defects...
2016: PeerJ
https://www.readbyqxmd.com/read/27920558/monitoring-the-effects-of-doxorubicin-on-3d-spheroid-tumor-cells-in-real-time
#7
NamHuk Baek, Ok Won Seo, MinSung Kim, John Hulme, Seong Soo A An
Recently, increasing numbers of cell culture experiments with 3D spheroids presented better correlating results in vivo than traditional 2D cell culture systems. 3D spheroids could offer a simple and highly reproducible model that would exhibit many characteristics of natural tissue, such as the production of extracellular matrix. In this paper numerous cell lines were screened and selected depending on their ability to form and maintain a spherical shape. The effects of increasing concentrations of doxorubicin (DXR) on the integrity and viability of the selected spheroids were then measured at regular intervals and in real-time...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27920473/na-hco3-cotransporter-is-expressed-on-%C3%AE-and-%C3%AE-cells-during-rat-pancreatic-development
#8
Li-Hua Cao, Cheng-Cai Xia, Zhao-Chun Shi, Ning Wang, Zheng-Hua Gu, Li-Zhi Yu, Qi Wan, Wei De
AIM: To determine the expression and localization of the electrogenic Na(+)/HCO3(-) cotransporter (NBC1) in rat pancreas during development. METHODS: The rat pancreas from postnatal and embryos removed from the uterus of pregnant rats that had been sacrificed by CO2 asphyxiation were used. Rat pancreas from embryonic day (E) 15.5 and E18.5 rat embryos was isolated under a stereomicroscope. Rat pancreas from postnatal (P) days 0, 7, 14, 21 and adult was directly isolated by the unaided eye...
November 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27920198/effects-of-breeder-age-strain-and-eggshell-temperature-on-nutrient-metabolism-of-broiler-embryos
#9
A Nangsuay, R Meijerhof, I van den Anker, M J W Heetkamp, B Kemp, H van den Brand
Breeder age and broiler strain influence the availability of nutrients and oxygen through yolk size and eggshell conductance, and the effects of these egg characteristics on nutrient metabolism might be influenced by eggshell temperature (EST). This study aims to determine effects of breeder age, strain, and EST on nutrient metabolism of embryos. A study was designed as a 2 × 2 × 2 factorial arrangement using four batches of in total 4,464 hatching eggs of 2 flock ages at 29 to 30 wk (young) and 54 to 55 wk (old) of Ross 308 and Cobb 500...
December 5, 2016: Poultry Science
https://www.readbyqxmd.com/read/27920155/sos2-and-acp1-loci-identified-through-large-scale-exome-chip-analysis-regulate-kidney-development-and-function
#10
Man Li, Yong Li, Olivia Weeks, Vladan Mijatovic, Alexander Teumer, Jennifer E Huffman, Gerard Tromp, Christian Fuchsberger, Mathias Gorski, Leo-Pekka Lyytikäinen, Teresa Nutile, Sanaz Sedaghat, Rossella Sorice, Adrienne Tin, Qiong Yang, Tarunveer S Ahluwalia, Dan E Arking, Nathan A Bihlmeyer, Carsten A Böger, Robert J Carroll, Daniel I Chasman, Marilyn C Cornelis, Abbas Dehghan, Jessica D Faul, Mary F Feitosa, Giovanni Gambaro, Paolo Gasparini, Franco Giulianini, Iris Heid, Jinyan Huang, Medea Imboden, Anne U Jackson, Janina Jeff, Min A Jhun, Ronit Katz, Annette Kifley, Tuomas O Kilpeläinen, Ashish Kumar, Markku Laakso, Ruifang Li-Gao, Kurt Lohman, Yingchang Lu, Reedik Mägi, Giovanni Malerba, Evelin Mihailov, Karen L Mohlke, Dennis O Mook-Kanamori, Antonietta Robino, Douglas Ruderfer, Erika Salvi, Ursula M Schick, Christina-Alexandra Schulz, Albert V Smith, Jennifer A Smith, Michela Traglia, Laura M Yerges-Armstrong, Wei Zhao, Mark O Goodarzi, Aldi T Kraja, Chunyu Liu, Jennifer Wessel, Eric Boerwinkle, Ingrid B Borecki, Jette Bork-Jensen, Erwin P Bottinger, Daniele Braga, Ivan Brandslund, Jennifer A Brody, Archie Campbell, David J Carey, Cramer Christensen, Josef Coresh, Errol Crook, Gary C Curhan, Daniele Cusi, Ian H de Boer, Aiko P J de Vries, Joshua C Denny, Olivier Devuyst, Albert W Dreisbach, Karlhans Endlich, Tõnu Esko, Oscar H Franco, Tibor Fulop, Glenn S Gerhard, Charlotte Glümer, Omri Gottesman, Niels Grarup, Vilmundur Gudnason, Tamara B Harris, Caroline Hayward, Lynne Hocking, Albert Hofman, Frank B Hu, Lise Lotte N Husemoen, Rebecca D Jackson, Torben Jørgensen, Marit E Jørgensen, Mika Kähönen, Sharon L R Kardia, Wolfgang König, Charles Kooperberg, Jennifer Kriebel, Lenore J Launer, Torsten Lauritzen, Terho Lehtimäki, Daniel Levy, Pamela Linksted, Allan Linneberg, Yongmei Liu, Ruth J F Loos, Antonio Lupo, Christine Meisinger, Olle Melander, Andres Metspalu, Paul Mitchell, Matthias Nauck, Peter Nürnberg, Marju Orho-Melander, Afshin Parsa, Oluf Pedersen, Annette Peters, Ulrike Peters, Ozren Polasek, David Porteous, Nicole M Probst-Hensch, Bruce M Psaty, Lu Qi, Olli T Raitakari, Alex P Reiner, Rainer Rettig, Paul M Ridker, Fernando Rivadeneira, Jacques E Rossouw, Frank Schmidt, David Siscovick, Nicole Soranzo, Konstantin Strauch, Daniela Toniolo, Stephen T Turner, André G Uitterlinden, Sheila Ulivi, Dinesh Velayutham, Uwe Völker, Henry Völzke, Melanie Waldenberger, Jie Jin Wang, David R Weir, Daniel Witte, Helena Kuivaniemi, Caroline S Fox, Nora Franceschini, Wolfram Goessling, Anna Köttgen, Audrey Y Chu
Genome-wide association studies have identified >50 common variants associated with kidney function, but these variants do not fully explain the variation in eGFR. We performed a two-stage meta-analysis of associations between genotypes from the Illumina exome array and eGFR on the basis of serum creatinine (eGFRcrea) among participants of European ancestry from the CKDGen Consortium (nStage1: 111,666; nStage2: 48,343). In single-variant analyses, we identified single nucleotide polymorphisms at seven new loci associated with eGFRcrea (PPM1J, EDEM3, ACP1, SPEG, EYA4, CYP1A1, and ATXN2L; PStage1<3...
December 5, 2016: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/27920088/suv4-20-activity-in-the-preimplantation-mouse-embryo-controls-timely-replication
#11
André Eid, Diego Rodriguez-Terrones, Adam Burton, Maria-Elena Torres-Padilla
Extensive chromatin remodeling after fertilization is thought to take place to allow a new developmental program to start. This includes dynamic changes in histone methylation and, in particular, the remodeling of constitutive heterochromatic marks such as histone H4 Lys20 trimethylation (H4K20me3). While the essential function of H4K20me1 in preimplantation mouse embryos is well established, the role of the additional H4K20 methylation states through the action of the SUV4-20 methyltransferases has not been addressed...
December 5, 2016: Genes & Development
https://www.readbyqxmd.com/read/27919701/evaluation-of-the-neurotoxic-effects-of-chronic-embryonic-exposure-with-inorganic-mercury-on-motor-and-anxiety-like-responses-in-zebrafish-danio-rerio-larvae
#12
Noraini Abu Bakar, Nurul Syafida Asma' Mohd Sata, Nurul Farhana Ramlan, Wan Norhamidah Wan Ibrahim, Syaizwan Zahmir Zulkifli, Che Azurahanim Che Abdullah, Mohammad Noor Amal Azmai
Chronic exposure to mercury (Hg) can lead to cumulative impairments in motor and cognitive functions including alteration in anxiety responses. Although several risk factors have been identified in recent year, little is known about the environmental factors that either due exposure toward low level of inorganic mercury that may led to the developmental disorders. The present study investigated the effects of embryonic exposure of mercury chloride on motor function and anxiety-like behavior. The embryo exposed to 6 different concentrations of HgCl2 (7...
December 2, 2016: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/27919666/calreticulin-is-a-secreted-bmp-antagonist-expressed-in-hensen-s-node-during-neural-induction
#13
Irene De Almeida, Nidia M M Oliveira, Rebecca A Randall, Caroline S Hill, John M McCoy, Claudio D Stern
Hensen's node is the "organizer" of the avian and mammalian early embryo. It has many functions, including neural induction and patterning of the ectoderm and mesoderm. Some of the signals responsible for these activities are known but these do not explain the full complexity of organizer activity. Here we undertake a functional screen to discover new secreted factors expressed by the node at this time of development. Using a Signal Sequence Trap in yeast, we identify several candidates. Here we focus on Calreticulin...
December 2, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27919529/three-step-effluent-chlorination-increases-disinfection-efficiency-and-reduces-dbp-formation-and-toxicity
#14
Yu Li, Xiangru Zhang, Mengting Yang, Jiaqi Liu, Wanxin Li, Nigel J D Graham, Xiaoyan Li, Bo Yang
Chlorination is extensively applied for disinfecting sewage effluents, but it unintentionally generates disinfection byproducts (DBPs). Using seawater for toilet flushing introduces a high level of bromide into domestic sewage. Chlorination of sewage effluent rich in bromide causes the formation of brominated DBPs. The objectives of achieving a disinfection goal, reducing disinfectant consumption and operational costs, as well as diminishing adverse effects to aquatic organisms in receiving water body remain a challenge in sewage treatment...
December 2, 2016: Chemosphere
https://www.readbyqxmd.com/read/27919228/antimicrobial-activity-and-safety-evaluation-of-peptides-isolated-from-the-hemoglobin-of-chickens
#15
Fengjiao Hu, Qiaoxing Wu, Shuang Song, Ruiping She, Yue Zhao, Yifei Yang, Meikun Zhang, Fang Du, Majid Hussain Soomro, Ruihan Shi
BACKGROUND: Hemoglobin is a rich source of biological peptides. As a byproduct and even wastewater of poultry-slaughtering facilities, chicken blood is one of the most abundant source of hemoglobin. RESULTS: In this study, the chicken hemoglobin antimicrobial peptides (CHAP) were isolated and the antimicrobial and bactericidal activities were tested by the agarose diffusion assay, minimum inhibitory concentration (MIC) analysis, minimal bactericidal concentration (MBC) analysis, and time-dependent inhibitory and bactericidal assays...
December 5, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27919171/cpg-methylation-participates-in-regulation-of-hepatitis-b-virus-gene-expression-in-host-sperm-and-sperm-derived-embryos
#16
ChengYao Zhong, Hui Lu, TingTing Han, XiaoFang Tan, PengHao Li, JiHua Huang, QingDong Xie, ZhiWei Hou, Ting Qu, Yang Jiang, Sheng Wang, Lan Xu, Ying Zhong, TianHua Huang
AIM: This study was undertaken to investigate relationship between hepatitis B virus (HBV) CpG methylation and HBV gene transcription in sperm and sperm-derived embryos. METHODS: HBV-infected patient sperm and HBV plasmid-transfected donor sperm were subjected to interspecific in vitro fertilization, methylation-specific PCR, bisulfite sequencing PCR, reverse transcription PCR and real-time quantitative PCR. RESULTS: Positive methylation bands for CpG islands II and III in the HBV genome were observed in patient sperm but not in controls, and methylation percentages of CpG sites varied among different patient sperm samples...
December 6, 2016: Epigenomics
https://www.readbyqxmd.com/read/27919073/mitochondrial-replacement-in-human-oocytes-carrying-pathogenic-mitochondrial-dna-mutations
#17
Eunju Kang, Jun Wu, Nuria Marti Gutierrez, Amy Koski, Rebecca Tippner-Hedges, Karen Agaronyan, Aida Platero-Luengo, Paloma Martinez-Redondo, Hong Ma, Yeonmi Lee, Tomonari Hayama, Crystal Van Dyken, Xinjian Wang, Shiyu Luo, Riffat Ahmed, Ying Li, Dongmei Ji, Refik Kayali, Cengiz Cinnioglu, Susan Olson, Jeffrey Jensen, David Battaglia, David Lee, Diana Wu, Taosheng Huang, Don P Wolf, Dmitry Temiakov, Juan Carlos Izpisua Belmonte, Paula Amato, Shoukhrat Mitalipov
Maternally inherited mitochondrial (mt)DNA mutations can cause fatal or severely debilitating syndromes in children, with disease severity dependent on the specific gene mutation and the ratio of mutant to wild-type mtDNA (heteroplasmy) in each cell and tissue. Pathogenic mtDNA mutations are relatively common, with an estimated 778 affected children born each year in the United States. Mitochondrial replacement therapies or techniques (MRT) circumventing mother-to-child mtDNA disease transmission involve replacement of oocyte maternal mtDNA...
November 30, 2016: Nature
https://www.readbyqxmd.com/read/27918914/transformation-of-jaw-muscle-satellite-cells-to-cardiomyocytes
#18
Randall S Daughters, Susan A Keirstead, Jonathan M W Slack
In the embryo a population of progenitor cells known as the second heart field forms not just parts of the heart but also the jaw muscles of the head. Here we show that it is possible to take skeletal muscle satellite cells from jaw muscles of the adult mouse and to direct their differentiation to become heart muscle cells (cardiomyocytes). This is done by exposing the cells to extracellular factors similar to those which heart progenitors would experience during normal embryonic development. By contrast, cardiac differentiation does not occur at all from satellite cells isolated from trunk and limb muscles, which originate from the somites of the embryo...
December 2, 2016: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/27918728/cholesterol-metabolism-in-rabbit-blastocysts-under-maternal-diabetes
#19
S Mareike Pendzialek, Maria Schindler, Torsten Plösch, Jacqueline Gürke, Elisa Haucke, Stefanie Hecht, Bernd Fischer, Anne Navarrete Santos
In the rabbit reproductive model, maternal experimentally induced insulin-dependent diabetes mellitus (expIDD) leads to accumulation of lipid droplets in blastocysts. Cholesterol metabolism is a likely candidate to explain such metabolic changes. Therefore, in the present study we analysed maternal and embryonic cholesterol concentrations and expression of related genes in vivo (diabetic model) and in vitro (embryo culture in hyperglycaemic medium). In pregnant expIDD rabbits, the serum composition of lipoprotein subfractions was changed, with a decrease in high-density lipoprotein cholesterol and an increase in very low-density lipoprotein cholesterol; in uterine fluid, total cholesterol concentrations were elevated...
December 6, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27918727/the-current-state-of-reproductive-biology-research-in-australia-and-new-zealand-core-themes-from-the-society-for-reproductive-biology-annual-meeting-2016
#20
L K Akison, P H Andraweera, M J Bertoldo, H M Brown, J S M Cuffe, T Fullston, O Holland, J E Schjenken
Because reproduction is essential for all life, it is central to our understanding of all aspects of biology. The Society for Reproductive Biology (SRB) 2016 conference held on the Gold Coast (Qld, Australia) displayed the current breadth of reproductive research in Australia and New Zealand, with additional insights from world leaders in the field. This conference review provides a focused summary of the key questions, emerging ideas and novel technologies that were presented in the symposia. Presented research demonstrated key advances in how stem cell biology may allow us to better understand pluripotency, as well as how environmental and lifestyle factors, such as circadian disruption, smoking, alcohol and diet, affect gametogenesis, embryo implantation, placental function and reproductive capacity...
December 6, 2016: Reproduction, Fertility, and Development
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