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https://www.readbyqxmd.com/read/28231693/native-plants-p-amurense-and-h-japonicus-extracts-act-as-antioxidants-to-support-developmental-competence-of-bovine-blastocysts
#1
Geon-Yeop Do, Jin-Woo Kim, Hyo-Jin Park, Seung-Bin Yoon, Jae-Young Park, Seul-Gi Yang, Bae Dong Jung, Yong-Soo Kwon, Man-Jong Kang, Bong-Seok Song, Sun-Uk Kim, Kyu-Tae Chang, Deog-Bon Koo
Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not yet been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extract on developmental competence and quality of preimplantation bovine embryos. Methods: After in vitro fertilization, bovine embryos were cultured for 7 days in CR1aa medium supplemented with P...
February 23, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28231245/signalling-crosstalk-at-the-leading-edge-controls-tissue-closure-dynamics-in-the-drosophila-embryo
#2
Raphaël Rousset, Fabrice Carballès, Nadège Parassol, Sébastien Schaub, Delphine Cérézo, Stéphane Noselli
Tissue morphogenesis relies on proper differentiation of morphogenetic domains, adopting specific cell behaviours. Yet, how signalling pathways interact to determine and coordinate these domains remains poorly understood. Dorsal closure (DC) of the Drosophila embryo represents a powerful model to study epithelial cell sheet sealing. In this process, JNK (JUN N-terminal Kinase) signalling controls leading edge (LE) differentiation generating local forces and cell shape changes essential for DC. The LE represents a key morphogenetic domain in which, in addition to JNK, a number of signalling pathways converges and interacts (anterior/posterior -AP- determination; segmentation genes, such as Wnt/Wingless; TGFβ/Decapentaplegic)...
February 23, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28231241/hydrocephalus-and-arthrogryposis-in-an-immunocompetent-mouse-model-of-zika-teratogeny-a-developmental-study
#3
Jose Xavier-Neto, Murilo Carvalho, Bruno Dos Santos Pascoalino, Alisson Campos Cardoso, Ângela Maria Sousa Costa, Ana Helena Macedo Pereira, Luana Nunes Santos, Ângela Saito, Rafael Elias Marques, Juliana Helena Costa Smetana, Silvio Roberto Consonni, Carla Bandeira, Vivian Vasconcelos Costa, Marcio Chaim Bajgelman, Paulo Sérgio Lopes de Oliveira, Marli Tenorio Cordeiro, Laura Helena Vega Gonzales Gil, Bianca Alves Pauletti, Daniela Campos Granato, Adriana Franco Paes Leme, Lucio Freitas-Junior, Carolina Borsoi Moraes Holanda de Freitas, Mauro Martins Teixeira, Estela Bevilacqua, Kleber Franchini
The teratogenic mechanisms triggered by ZIKV are still obscure due to the lack of a suitable animal model. Here we present a mouse model of developmental disruption induced by ZIKV hematogenic infection. The model utilizes immunocompetent animals from wild-type FVB/NJ and C57BL/6J strains, providing a better analogy to the human condition than approaches involving immunodeficient, genetically modified animals, or direct ZIKV injection into the brain. When injected via the jugular vein into the blood of pregnant females harboring conceptuses from early gastrulation to organogenesis stages, akin to the human second and fifth week of pregnancy, ZIKV infects maternal tissues, placentas and embryos/fetuses...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28230329/integrating-trans-abdominal-ultrasonography-with-fecal-steroid-metabolite-monitoring-to-accurately-diagnose-pregnancy-and-predict-the-timing-of-parturition-in-the-red-panda-ailurus-fulgens-styani
#4
Erin Curry, Lissa J Browning, Paul Reinhart, Terri L Roth
Red pandas (Ailurus fulgens styani) exhibit a variable gestation length and may experience a pseudopregnancy indistinguishable from true pregnancy; therefore, it is not possible to deduce an individual's true pregnancy status and parturition date based on breeding dates or fecal progesterone excretion patterns alone. The goal of this study was to evaluate the use of transabdominal ultrasonography for pregnancy diagnosis in red pandas. Two to three females were monitored over 4 consecutive years, generating a total of seven profiles (four pregnancies, two pseudopregnancies, and one lost pregnancy)...
February 23, 2017: Zoo Biology
https://www.readbyqxmd.com/read/28230233/establishment-and-growth-responses-of-nile-tilapia-embryonic-stem-like-cell-lines-under-feeder-free-condition
#5
Zhenhua Fan, Linyan Liu, Xiaohuan Huang, Yang Zhao, Linyan Zhou, Deshou Wang, Jing Wei
Embryonic stem (ES) cells provide an invaluable tool for molecular analysis of vertebrate development and a bridge linking genomic manipulations in vitro and functional analysis of target genes in vivo. Work towards fish ES cells so far has focused on zebrafish (Danio renio) and medaka (Oryzias latipes). Here we describe the derivation, pluripotency, differentiation and growth responses of ES cell lines from Nile tilapia (Oreochromis niloticus), a world-wide commercial farmed fish. These cell lines, designated as TES1-3, were initiated from blastomeres of Nile tilapia middle blastula embryos (MBE)...
February 23, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28229988/endometrial-microrna-signature-during-the-window-of-implantation-changed-in-patients-with-repeated-implantation-failure
#6
Cheng Shi, Huan Shen, Li-Juan Fan, Jing Guan, Xin-Bang Zheng, Xi Chen, Rong Liang, Xiao-Wei Zhang, Qing-Hua Cui, Kun-Kun Sun, Zhu-Ran Zhao, Hong-Jing Han
BACKGROUND: At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different endometrial microRNA (miRNA) signatures for impaired endometrial receptivity by microarray analysis. METHODS: A total of 12 repeated implantation failure (RIF) patients and 10 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28229951/chemical-and-toxicological-characterizations-of-hydraulic-fracturing-flowback-and-produced-water
#7
Yuhe He, Shannon L Flynn, Erik J Folkerts, Yifeng Zhang, Dongliang Ruan, Daniel S Alessi, Jonathan W Martin, Greg G Goss
Hydraulic fracturing (HF) has emerged as a major method of unconventional oil and gas recovery. The toxicity of hydraulic fracturing flowback and produced water (HF-FPW) has not been previously reported and is complicated by the combined complexity of organic and inorganic constituents in HF fluids and deep formation water. In this study, we characterized the solids, salts, and organic signatures in an HF-FPW sample from the Duvernay Formation, Alberta, Canada. Untargeted HPLC-Orbitrap revealed numerous unknown dissolved polar organics...
February 14, 2017: Water Research
https://www.readbyqxmd.com/read/28229028/small-scale-spatial-variation-in-population-and-individual-level-reproductive-parameters-of-the-blue-legged-hermit-crab-clibanarius-tricolor
#8
J Antonio Baeza, Donald C Behringer
Management of the few regulated ornamental fisheries relies on inadequate information about the life history of the target species. Herein, we investigated the reproductive biology of the most heavily traded marine invertebrate in the western Atlantic; the blue-legged hermit crab Clibanarius tricolor. We report on density, individual-level, and population-level reproductive parameters in 14 populations spanning the Florida Keys. In C. tricolor, abundance, population-level, and individual-level reproductive parameters exhibited substantial small-scale spatial variation in the Florida Keys...
2017: PeerJ
https://www.readbyqxmd.com/read/28228767/identification-of-zoupi-orthologs-in-soybean-potentially-involved-in-endosperm-breakdown-and-embryogenic-development
#9
Yaohua Zhang, Xin Li, Suxin Yang, Xianzhong Feng
Soybean (Glycine max Merr.) is the world's most widely grown legume and provides an important source of protein and oil. Improvement of seed quality requires deep insights into the genetic regulation of seed development. The endosperm serves as a temporary source of nutrients that are transported from maternal to filial tissues, and it also generates signals for proper embryo formation. Endosperm cell death is associated with the processes of nutrient transfer and embryo expansion. The bHLH domain transcription factor AtZHOUPI (AtZOU) plays a key role in both the lysis of the transient endosperm and the formation of embryo cuticle in Arabidopsis thaliana...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28228749/neuroprotective-role-of-the-pi3-kinase-akt-signaling-pathway-in-zebrafish
#10
Shuang Chen, Yunzhang Liu, Xiaozhi Rong, Yun Li, Jianfeng Zhou, Ling Lu
Neuronal survival and growth in the embryo is controlled partly by trophic factors. For most trophic factors (such as Insulin-like growth factor-1), the ability to regulate cell survival has been attributed to the phosphoinositide 3-kinase (PI3K)/Akt kinase cascade. This study presents data illustrating the role of PI3K/Akt in attainment of normal brain size during zebrafish embryogenesis. Blocking PI3K with inhibitor LY294002 caused a significant reduction in brain size (in addition to global growth retardation) during zebrafish embryogenesis...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28228731/increased-hemodynamic-load-in-early-embryonic-stages-alters-endocardial-to-mesenchymal-transition
#11
Madeline Midgett, Claudia S López, Larry David, Alina Maloyan, Sandra Rugonyi
Normal blood flow is essential for proper heart formation during embryonic development, as abnormal hemodynamic load (blood pressure and shear stress) results in cardiac defects seen in congenital heart disease. However, the progressive detrimental remodeling processes that relate altered blood flow to cardiac defects remain unclear. Endothelial-mesenchymal cell transition is one of the many complex developmental events involved in transforming the early embryonic outflow tract into the aorta, pulmonary trunk, interventricular septum, and semilunar valves...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28228439/loss-of-pollen-specific-phospholipase-not-like-dad-triggers-gynogenesis-in-maize
#12
Laurine M Gilles, Abdelsabour Khaled, Jean-Baptiste Laffaire, Sandrine Chaignon, Ghislaine Gendrot, Jérôme Laplaige, Hélène Bergès, Genséric Beydon, Vincent Bayle, Pierre Barret, Jordi Comadran, Jean-Pierre Martinant, Peter M Rogowsky, Thomas Widiez
Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE DAD (NLD) coding for a patatin-like phospholipase A. In all surveyed inducer lines, NLD carries a 4-bp insertion leading to a predicted truncated protein...
February 22, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28228401/wolf-hirschhorn-syndrome-candidate-1-like-1-epigenetically-regulates-nephrin-gene-expression
#13
Yugo Ito, Kan Katayama, Yukino Nishibori, Yoshihiro Akimoto, Akihiko Kudo, Ryota Kurayama, Ichiro Hada, Shohei Takahashi, Toru Kimura, Toshiyuki Fukutomi, Tomohisa Katada, Junichi Suehiro, Olga Beltcheva, Karl Tryggvason, Kunimasa Yan
Altered expression of nephrin underlies the pathophysiology of proteinuria in both congenital and acquired nephrotic syndrome. However, the epigenetic mechanisms of nephrin gene regulation remain elusive. Here, we show that Wolf-Hirschhorn syndrome candidate 1-like 1 long form (WHSC1L1-L) is a novel epigenetic modifier of nephrin gene regulation. WHSC1L1-L was associated with histone H3K4 and H3K36 in human embryonic kidney cells. WHSC1L1-L gene was expressed in the podocytes and functional protein product was detected in these cells...
February 22, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28228320/embryo-multinucleation-at-the-two-cell-stage-is-an-independent-predictor-of-intracytoplasmic-sperm-injection-outcomes
#14
Laurent Desch, Céline Bruno, Maxime Luu, Julie Barberet, Cécile Choux, Marjorie Lamotte, Emeline Schmutz, Paul Sagot, Patricia Fauque
OBJECTIVE: To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN: Retrospective study. SETTING: Hospital. PATIENT(S): Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28228316/gonadotropin-releasing-hormone-analogue-as-sole-luteal-support-in-antagonist-based-assisted-reproductive-technology-cycles
#15
Itai Bar Hava, Moran Blueshtein, Hadas Ganer Herman, Yeela Omer, Gila Ben David
OBJECTIVE: To evaluate the efficacy of GnRH agonists (GnRH-a) as sole luteal phase support in patients undergoing IVF in antagonist-based cycles compared with standard vaginal P preparations. DESIGN: Retrospective cohort. SETTING: Private fertility clinic. PATIENT(S): Patients who underwent antagonist-based cycles performed at our clinic between 2009 and 2015. INTERVENTION(S): Intranasal GnRH-a or vaginal P as luteal support...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28227372/multimodal-embryonic-imaging-using-optical-coherence-tomography-selective-plane-illumination-microscopy-and-optical-projection-tomography
#16
M Singh, C Wu, D Mayerich, M E Dickinson, I V Larina, K V Larin, M Singh, C Wu, D Mayerich, M E Dickinson, I V Larina, K V Larin, I V Larina, M Singh, K V Larin, D Mayerich, M E Dickinson, C Wu
The murine model is commonly utilized for studying developmental diseases. Different optical techniques have been developed to image mouse embryos, but each has its own set of limitations and restrictions. In this study, we compare the performance of the well-established technique of optical coherence tomography (OCT) to the relatively new methods of selective plane illumination microscopy (SPIM) and optical projection tomography (OPT) to assess murine embryonic development. OCT can provide label free high resolution images of the mouse embryo, but suffers from light attenuation that limits visualization of deeper structures...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226725/a-chicken-embryo-cardiac-outflow-tract-atlas-for-registering-changes-due-to-abnormal-blood-flow
#17
James P Carson, Monique Y Rennie, Michael Danilchik, Kent Thornburg, Sandra Rugonyi, James P Carson, Monique Y Rennie, Michael Danilchik, Kent Thornburg, Sandra Rugonyi, Kent Thornburg, Michael Danilchik, James P Carson, Monique Y Rennie, Sandra Rugonyi
Subdivision-based image registration has previously been applied to co-localize digital information extracted from rigid structures in biological specimens, such as the brain. Here, we describe and demonstrate the creation and application of a two-dimensional subdivision-based atlas representing a dynamic structure: the outflow tract of the developing chicken heart. The atlas is designed to segment three different anatomical layers of the outflow tract, and is demonstrated on the characterization of collagen XIV in both control and induced abnormal flow specimens...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226539/directing-the-spatial-patterning-of-motor-neuron-differentiation-in-engineered-microenvironments
#18
Christopher J Demers, Greg Cox, Scott D Collins, Rosemary L Smith, Christopher J Demers, Greg Cox, Scott D Collins, Rosemary L Smith, Greg Cox, Christopher J Demers, Scott D Collins, Rosemary L Smith
Embryonic development of the spinal cord proceeds through a carefully orchestrated temporal and spatial sequence of chemical cues to provide precise patterning of adult cell types. Recreating this complex microenvironment in a standard cell culture dish is difficult, if not impossible. In this paper, a microfluidic device is used to recapitulate, in vitro, the graded patterning events which occur during early spinal cord development. The microdevice design is developed using COMSOL modeling, with which the spatiotemporal profiles of multiple, diffusible morphogens are simulated...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226240/position-and-hippo-signaling-dependent-plasticityduring-lineage-segregation-in-the-early-mouse-embryo
#19
Eszter Posfai, Sophie Petropoulos, Flavia Ro de Barros, John Paul Schell, Igor Jurisica, Rickard Sandberg, Fredrik Lanner, Janet Rossant
The segregation of the trophectoderm (TE) from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo signaling. However, the window of responsiveness to Hippo signaling, the exact timing of lineage commitment and the overall relationship between cell commitment and global gene expression changes are still unclear. Single-cell RNA sequencing during lineage segregation revealed that the TE transcriptional profile stabilizes earlier than the ICM and prior to blastocyst formation...
February 22, 2017: ELife
https://www.readbyqxmd.com/read/28225561/flotillins-control-zebrafish-epiboly-through-their-role-in-cadherin-mediated-cell-cell-adhesion
#20
Eduardo A Rios Morris, Stéphane Bodin, Bénédicte Delaval, Franck Comunale, Virginie Georget, Manoel L Costa, Georges Lutfalla, Cécile Gauthier-Rouvière
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions...
February 22, 2017: Biology of the Cell
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