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https://www.readbyqxmd.com/read/28432834/molecular-control-of-gut-formation-in-the-spider-parasteatoda-tepidariorum
#1
Natália Martins Feitosa, Matthias Pechmann, Evelyn E Schwager, Vitória Tobias-Santos, Alistair P McGregor, Wim G M Damen, Rodrigo Nunes da Fonseca
The development of a digestive system is an essential feature of bilaterians. Studies of the molecular control of gut formation in arthropods have been studied in detail in the fruit fly Drosophila melanogaster. However, little is known in other arthropods, especially in noninsect arthropods. To better understand the evolution of arthropod alimentary system, we investigate the molecular control of gut development in the spider Parasteatoda tepidariorum (Pt), the primary chelicerate model species for developmental studies...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432826/ploidy-has-little-effect-on-timing-early-embryonic-events-in-the-haplo-diploid-wasp-nasonia
#2
Deanna Arsala, Jeremy A Lynch
The nucleocytoplasmic (N/C) ratio plays a prominent role in the maternal-to-zygotic transition (MZT) in many animals. The effect of the N/C ratio on cell-cycle lengthening and zygotic genome activation (ZGA) has been studied extensively in Drosophila, where haploid embryos experience an additional division prior to completing cellularization and triploid embryos cellularize precociously by one division. In this study, we set out to understand how the obligate difference in ploidy in the haplodiploid wasp, Nasonia, affects the MZT and which aspects of the Drosophila MZT are conserved...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432817/factors-involved-in-early-polarization-of-the-anterior-posterior-axis-in-the-milkweed-bug-oncopeltus-fasciatus
#3
Neta Ginzburg, Mira Cohen, Ariel D Chipman
The axes of insect embryos are defined early in the blastoderm stage. Genes involved in this polarization are well known in Drosophila, but less so in other insects, such as the milkweed bug Oncopeltus fasciatus. Using quantitative PCR, we looked at differential expression of several candidate genes for early anterior-posterior patterning and found that none of them are expressed asymmetrically in the early blastoderm. We then used an RNA-Seq approach to identify novel candidate genes that might be involved in early polarization in Oncopeltus...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432816/rhodnius-prolixus-from-classical-physiology-to-modern-developmental-biology
#4
REVIEW
Rodrigo Nunes-da-Fonseca, Mateus Berni, Vitória Tobias-Dos-Santos, Attilio Pane, Helena Marcolla Araujo
The hemiptera Rhodnius prolixus is a blood-feeding insect and a primary vector of Trypanosoma cruzi, the etiological agent of the Chagas disease. Over the past century, Rhodnius has been the subject of intense investigations, which have contributed to unveil important aspects of metabolism and physiology in insects. Recent technological innovations are helping dissect the genetic and molecular underpinnings of Rhodnius embryogenesis and organogenesis, thus fostering the use of this important species in the fields of developmental and evolutionary biology...
April 22, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28432708/in-vitro-production-of-porcine-zygotes-using-intracytoplasmic-injection-of-vitrified-sperm
#5
C C Arraztoa, C Baca Castex, G M Alvarez, P D Cetica, D M Neild
The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO2 and tri-gas. A group of porcine oocytes maturated in vitro were injected with vitrified-warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test...
April 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28432707/suberoylanilide-hydroxamic-acid-a-novel-histone-deacetylase-inhibitor-improves-the-development-and-acetylation-level-of-miniature-porcine-handmade-cloning-embryos
#6
J M Sun, K Q Cui, Z P Li, X R Lu, Z F Xu, Q Y Liu, B Huang, D S Shi
Histone deacetylase inhibitors (HDACis) can change the histone acetylation and significantly enhance the developmental competence of the pre-implantation SCNT embryo. To select a proper histone deacetylase inhibitor to improve the success rate and potentially developmental ability of handmade cloning (HMC) embryos of miniature porcine, we compared the effect of two histone deacetylase inhibitors (SAHA vs. VPA) on HMC embryo development, their histone acetylation level and the expression level of relevant genes...
April 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28432599/the-beneficial-effect-of-repaglinide-on-in-vitro-maturation-and-development-ability-of-immature-mouse-oocytes
#7
Eshrat Kalehoei, Mehri Azadbakht
Repaglinide is a hypoglycemic drug, causing depolarization of the cell membrane, opening the voltage-gated calcium channels, and then increasing intracellular calcium in the pancreatic B cells by inhibition of the K-ATP-sensitive channels. Oocyte in vitro maturation (IVM) is influenced by different factors such as calcium signaling. In this study, we examined the effects of repaglinide on in vitro maturation and fertilization ability of mouse oocyte. Immature oocytes were isolated from female Naval Medical Research Institute mice which are 6-8 wk old mechanically and then cultured in 30 μl droplets of T6 medium with different concentrations of repaglinide...
April 21, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28432496/reproductive-effects-of-life-cycle-exposure-to-difenoconazole-on-female-marine-medaka-oryzias-melastigma
#8
Xiaocui Dong, Zhenghong Zuo, Jiaojiao Guo, Hongbin Li, Lemeng Zhang, Meng Chen, Zhibin Yang, Chonggang Wang
Difenoconazole (DFZ) is a widely used triazole fungicide which has been detected in some estuaries and embayments. This study was conducted to investigate the effects of DFZ on ovarian development in female marine medaka (Oryzias melastigma). After 180 days exposure of the embryo to DFZ (0, 1, 10, 100 and 1000 ng/L), the gonadosomatic index and percentage of mature oocytes produced were significantly reduced in the 1, 10 and 100 ng/L treatments but not the 1000 ng/L treatment compared to the control, thus exhibiting a U-shaped dose response curve...
April 21, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28432333/metabolomic-and-proteomic-analysis-of-maize-embryonic-callus-induced-from-immature-embryo
#9
Fei Ge, Hongmei Hu, Xing Huang, Yanling Zhang, Yanli Wang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen
The low ratio of embryonic callus (EC) induction has inhibited the rapid development of maize genetic engineering. Still, little is known to explain the genotype-dependence of EC induction. Here, we performed a large-scale, quantitative analysis of the maize EC metabolome and proteome at three typical induction stages in two inbred lines with a range of EC induction capabilities. Comparison of the metabolomes and proteomes suggests that the differential molecular responses begin at an early stage of development and continue throughout the process of EC formation...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432219/optimized-cubic-protocol-for-3d-imaging-of-chicken-embryos-at-single-cell-resolution
#10
María Victoria Gómez-Gaviro, Evan Balaban, Diana Bocancea, María Teresa Lorrio, María Pompeiano, Manuel Desco, Jorge Ripoll, Juan José Vaquero
The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole early-stage chicken embryos, and in the whole brains of late-stage embryos.
April 21, 2017: Development
https://www.readbyqxmd.com/read/28432216/embryonic-cholecystitis-and-defective-gallbladder-contraction-in-the-sox17-haploinsufficient-model-of-biliary-atresia
#11
Hiroki Higashiyama, Aisa Ozawa, Hiroyuki Sumitomo, Mami Uemura, Ko Fujino, Hitomi Igarashi, Kenya Imaimatsu, Naoki Tsunekawa, Yoshikazu Hirate, Masamichi Kurohmaru, Yukio Saijoh, Masami Kanai-Azuma, Yoshiakira Kanai
The gallbladder excretes cytotoxic bile acids to the duodenum through the cystic duct and common bile duct system. Sox17 haploinsufficiency causes the biliary atresia-like phenotypes and hepatitis in late organogenesis mouse embryos. However, the molecular and cellular mechanisms in the Sox17-haploinsufficient gallbladder and liver in the etiology of biliary atresia remain unclear. In this study, transcriptomic analyses revealed the early onset of cholecystitis in the Sox17(+/-) embryos, together with the appearance of ectopic cystic duct-like epithelia in their gallbladders...
April 21, 2017: Development
https://www.readbyqxmd.com/read/28432215/actomyosin-based-tissue-folding-requires-a-multicellular-myosin-gradient
#12
Natalie C Heer, Pearson W Miller, Soline Chanet, Norbert Stoop, Jörn Dunkel, Adam C Martin
Tissue folding promotes three-dimensional (3D) form during development. In many cases, folding is associated with myosin accumulation at the apical surface of epithelial cells, as seen in the vertebrate neural tube and the Drosophila ventral furrow. This type of folding is characterized by constriction of apical cell surfaces, and the resulting cell shape change is thought to cause tissue folding. Here, we use quantitative microscopy to measure the pattern of transcription, signaling, myosin activation, and cell shape in the Drosophila mesoderm...
April 21, 2017: Development
https://www.readbyqxmd.com/read/28432198/folate-dependent-methylation-of-septins-governs-ciliogenesis-during-neural-tube-closure
#13
Manami Toriyama, Michinori Toriyama, John B Wallingford, Richard H Finnell
Periconception maternal folic acid (vitamin B9) supplementation can reduce the prevalence of neural tube defects (NTDs), although just how folates benefit the developing embryo and promote closing of the neural tube and other morphologic processes during development remains unknown. Folate contributes to a 1-carbon metabolism, which is essential for purine biosynthesis and methionine recycling and affects methylation of DNA, histones, and nonhistone proteins. Herein, we used animal models and cultured mammalian cells to demonstrate that disruption of the methylation pathway mediated by folate compromises normal neural tube closure (NTC) and ciliogenesis...
April 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28432103/morphology-and-cardiac-physiology-are-differentially-affected-by-temperature-in-developing-larvae-of-the-marine-fish-mahi-mahi-coryphaena-hippurus
#14
Prescilla Perrichon, Christina Pasparakis, Edward M Mager, John D Stieglitz, Daniel D Benetti, Martin Grosell, Warren W Burggren
Cardiovascular performance is altered by temperature in larval fishes, but how acute vs. chronic temperature exposures independently affect cardiac morphology and physiology in the growing larva is poorly understood. Consequently, the influence of water temperature on cardiac plasticity was investigated in developing mahi-mahi. Morphological (e.g. standard length, heart angle) and physiological cardiac variables (e.g. heart rate fH, stroke volume, cardiac output) were recorded under two conditions by imaging: i) under acute temperature exposure where embryos were reared at 25°C up to 128 hours post-fertilization (hpf) and then acutely exposed to 25° (rearing temperature), 27° and 30°C; ii) at two rearing (chronic) temperatures of 26° and 30°C and performed at 32 and 56 hpf...
April 21, 2017: Biology Open
https://www.readbyqxmd.com/read/28431985/quercetin-interferes-with-the-fluid-volume-and-receptivity-development-of-the-uterus-in-rats-during-the-peri-implantation-period
#15
Huma Shahzad, Nelli Giribabu, Kamarulzaman Karim, Normadiah Kassim, Sekaran Muniandy, Kilari Eswar Kumar, Naguib Salleh
HYPOTHESIS: quercetin could induce changes to the fluid volume and receptivity development of the uterus during peri-implantation period. METHODS: Female rats were treated with quercetin (10, 25 and 50mg/kg/day) subcutaneously beginning from day-1 pregnancy. Uterus was harvested at day-4 (following three days quercetin treatment) for morphological, ultra-structural, protein and mRNA expressional changes and plasma sex-steroid levels analyses. In another cohort of rats, implantation rate was determined at day-6 (following five days quercetin treatment)...
April 18, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28431894/molecular-cloning-and-functional-characterization-of-duck-nucleotide-binding-oligomerization-domain-1-nod1
#16
Huilin Li, Hui Jin, Yaqian Li, Dejian Liu, Mohamed Frahat Foda, Yunbo Jiang, Rui Luo
Nucleotide-binding oligomerization domain 1 (NOD1) is an imperative cytoplasmic pattern recognition receptor (PRR) and considered as a key member of the NOD-like receptor (NLR) family which plays a critical role in innate immunity through sensing microbial components derived from bacterial peptidoglycan. In the current study, the full-length of duck NOD1 (duNOD1) cDNA from duck embryo fibroblasts (DEFs) was cloned. Multiple sequence alignment and phylogenetic analysis demonstrated that duNOD1 exhibited a strong evolutionary relationship with chicken and rock pigeon NOD1...
April 18, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28431857/modeling-human-infertility-with-pluripotent-stem-cells
#17
Di Chen, Joanna J Gell, Yu Tao, Enrique Sosa, Amander T Clark
Human fertility is dependent upon the correct establishment and differentiation of the germline. This is because no other cell type in the body is capable of passing a genome and epigenome from parent to child. Terminally differentiated germline cells in the adult testis and ovary are called gametes. However, the initial specification of germline cells occurs in the embryo around the time of gastrulation. Most of our knowledge regarding the cell and molecular events that govern human germline specification involves extrapolating scientific principles from model organisms, most notably the mouse...
April 13, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28431828/clinical-use-of-monopronucleated-zygotes-following-blastocyst-culture-and-preimplantation-genetic-screening-including-verification-of-biparental-chromosome-inheritance
#18
Cara K Bradley, Maria Traversa, Natalie Hobson, Alison J Gee, Steven J McArthur
In assisted reproduction, embryos derived from monopronucleated (1PN) zygotes are considered abnormal and unsuitable for clinical use. Outcomes of 1PN-derived embryos designated for preimplantation genetic screening (PGS) were analysed. These embryos, especially from intracytoplasmic sperm injection (ICSI), were found to have a low developmental potential; 1PN and 2PN day 5 blastocyst development for IVF was 14.8% versus 36.4% (P < 0.0001), and for ICSI, 6.6% versus 34.0% (P < 0.0001), respectively. With the use of comparative genomic hybridization or next-generation sequencing, PGS was successfully carried out for 74 IVF and 32 ICSI 1PN-derived blastocysts, revealing adjusted abnormality rates of 39...
March 27, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28431082/compositional-differences-among-female-associated-and-embryo-associated-microbiota-of-the-viviparous-pacific-beetle-cockroach-diploptera-punctata
#19
Paul A Ayayee, George Keeney, Zakee L Sabree, Agustí Muñoz-Garcia
All cockroach species, except one, harbor the endosymbiont Blattabacterium, transmitted from females to embryos. Adult cockroaches acquire non-Blattabacterium bacteria as part of their gut microbiota over time, but our knowledge of the possible transmission of these non-Blattabacterium bacteria from females to embryos is rudimentary. We characterized the gut microbiota of gravid viviparous Diploptera punctata females and the non-Blattabacterium microbiota of associated developing embryos, as well as the gut microbiota of non-gravid females, and the microbiota of orphan embryos (females not included), following high-throughput sequencing of the 16S rRNA gene to assess bacterial transference...
April 18, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28431017/long-non-coding-rna-exchange-during-the-oocyte-to-embryo-transition-in-mice
#20
Rosa Karlic, Sravya Ganesh, Vedran Franke, Eliska Svobodova, Jana Urbanova, Yutaka Suzuki, Fugaku Aoki, Kristian Vlahovicek, Petr Svoboda
No abstract text is available yet for this article.
April 1, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
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