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https://www.readbyqxmd.com/read/28545549/detection-and-genetic-characterization-of-a-wide-range-of-infectious-agents-in-ixodes-pavlovskyi-ticks-in-western-siberia-russia
#1
Vera Rar, Natalia Livanova, Sergey Tkachev, Galina Kaverina, Artem Tikunov, Yuliya Sabitova, Yana Igolkina, Victor Panov, Stanislav Livanov, Nataliya Fomenko, Igor Babkin, Nina Tikunova
BACKGROUND: The Ixodes pavlovskyi tick species, a member of the I. persulcatus/I. ricinus group, was discovered in the middle of the 20(th) century in the Russian Far East. Limited data have been reported on the detection of infectious agents in this tick species. The aim of this study was to investigate the prevalence and genetic variability of a wide range of infectious agents in I. pavlovskyi ticks collected in their traditional and recently invaded habitats, the Altai Mountains and Novosibirsk Province, respectively, which are both located within the Western Siberian part of the I...
May 25, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28543264/implication-clinical-and-biological-impact-of-vector-borne-haemopathogens-in-anaemic-dogs-in-france-a-prospective-study
#2
T Bouzouraa, J L Cadore, J Chene, I Goy-Thollot, F Ponce, K Chalvet-Monfray, B Rannou, L Chabanne
OBJECTIVES: To prospectively describe the clinical and biological impact of vector-borne haemopathogens in anaemic dogs in France and occurrence of haemolysis. MATERIALS AND METHODS: This prospective descriptive cohort study includes 134 client-owned dogs that were anaemic on admission at the Veterinary Teaching Hospital of VetAgro Sup, Lyon, France. They underwent comprehensive screening with PCR to detect a panel of vector-borne haemopathogens, SNAP Leishmania and SNAP 4Dx Plus (IDEXX)...
May 23, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28541469/clinical-and-molecular-evidence-of-atovaquone-and-azithromycin-resistance-in-relapsed-babesia-microti-infection-associated-with-rituximab-and-chronic-lymphocytic-leukemia
#3
Matthew S Simon, Lars F Westblade, Alexis Dziedziech, Joseph E Visone, Richard R Furman, Stephen G Jenkins, Audrey N Schuetz, Laura A Kirkman
Babesiosis treatment failures with standard therapy have been reported, but the molecular mechanisms are not well understood. We describe the emergence of atovaquone and azithromycin resistance associated with mutations in the binding regions of the target proteins of both drugs during treatment of an immunosuppressed patient with relapsing babesiosis.
May 24, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28536802/molecular-investigations-of-the-bat-tick-argas-vespertilionis-ixodida-argasidae-and-babesia-vesperuginis-apicomplexa-piroplasmida-reflect-bat-connection-between-central-europe-and-central-asia
#4
Sándor Hornok, Krisztina Szőke, Tamás Görföl, Gábor Földvári, Vuong Tan Tu, Nóra Takács, Jenő Kontschán, Attila D Sándor, Péter Estók, Sara Epis, Sándor Boldogh, Dávid Kováts, Yuanzhi Wang
Argas vespertilionis is a geographically widespread haematophagous ectoparasite species of bats in the Old World, with a suspected role in the transmission of Babesia vesperuginis. The aims of the present study were (1) to molecularly screen A. vespertilionis larvae (collected in Europe, Africa and Asia) for the presence of piroplasms, and (2) to analyze mitochondrial markers of A. vespertilionis larvae from Central Asia (Xinjiang Province, Northwestern China) in a phylogeographical context. Out of the 193 DNA extracts from 321 A...
May 23, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28532427/microrna-275-and-its-target-vitellogenin-2-are-crucial-in-ovary-development-and-blood-digestion-of-haemaphysalis-longicornis
#5
Jiawei Hao, Jin Luo, Ze Chen, Qiaoyun Ren, Jinxia Guo, Xiaocui Liu, Qiuyu Chen, Feng Wu, Zhen Wang, Jianxun Luo, Hong Yin, Hui Wang, Guangyuan Liu
BACKGROUND: The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens...
May 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28514953/emergence-of-babesia-canis-in-southern-england
#6
Maria Del Mar Fernández de Marco, Luis M Hernández-Triana, L Paul Phipps, Kayleigh Hansford, E Sian Mitchell, Ben Cull, Clive S Swainsbury, Anthony R Fooks, Jolyon M Medlock, Nicholas Johnson
BACKGROUND: The United Kingdom is considered free of autochthonous transmission of canine babesiosis although cases are reported in dogs associated with recent travel abroad. During the winter months of 2015/16, a cluster of cases of disease in dogs with signs suggestive of canine babesiosis were reported in Harlow, Essex. METHODS: Babesia species were detected in dog blood samples by Giemsa staining of blood smears and by pan-piroplasm PCRs. Babesia species were also detected in extracts of tick DNA using pan-piroplasm PCRs...
May 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28506045/serological-and-molecular-detection-of-toxoplasma-gondii-and-babesia-microti-in-the-blood-of-rescued-wild-animals-in-gangwon-do-province-korea
#7
Sung-Hee Hong, Hee-Jong Kim, Young-Il Jeong, Shin-Hyeong Cho, Won-Ja Lee, Jong-Tak Kim, Sang-Eun Lee
Infections of Toxoplasma gondii and Babesia microti are reported in many wild animals worldwide, but information on their incidence and molecular detection in Korean wild fields is limited. In this study, the prevalence of T. gondii and B. microti infection in blood samples of 5 animal species (37 Chinese water deer, 23 raccoon dogs, 6 roe deer, 1 wild boar, and 3 Eurasian badgers) was examined during 2008-2009 in Gangwon-do (Province), the Republic of Korea (=Korea) by using serological and molecular tests...
April 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28504633/-tick-borne-infections-in-denmark
#8
Bo Bødker Jensen, Lukas Frans Ocias, Nanna Skaarup Andersen, Ram Benny Dessau, Karen Angeliki Krogfelt, Sigurdur Skarphedinsson
The castor bean tick, Ixodes ricinus, is common in woodlands in most of Denmark. Besides Borrelia burgdorferi, it can harbour a number of pathogenic microorganisms such as tick-borne encephalitis virus, Anaplasma phagocytophilum, Rickettsia helvetica, Francisella tularensis, Candidatus Neoehrlichia mikurensis, Bartonella spp., Borrelia miyamotoi and Babesia spp. These tick-borne infections should be a differential diagnostic consideration during the tick season in Denmark. We review the distribution, clinical manifestations, diagnosis and treatment of these microorganisms...
May 15, 2017: Ugeskrift for Laeger
https://www.readbyqxmd.com/read/28504439/anaplasma-phagocytophilum-babesia-microti-borrelia-burgdorferi-sensu-lato-and-toxoplasma-gondii-in-ixodes-ricinus-acari-ixodida-ticks-collected-from-slowinski-national-park-northern-poland
#9
Marek Asman, Magdalena Nowak-Chmura, Krzysztof Solarz, Ewa Szilman, Magdalena Semla, Bartłomiej Zyśk
No abstract text is available yet for this article.
June 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/28502642/molecular-detection-of-tick-borne-pathogens-in-wild-red-foxes-vulpes-vulpes-from-central-italy
#10
Valentina Virginia Ebani, Guido Rocchigiani, Simona Nardoni, Fabrizio Bertelloni, Violetta Vasta, Roberto Amerigo Papini, Ranieri Verin, Alessandro Poli, Francesca Mancianti
Spleen samples from 153 red foxes, shot during regular hunting season in the province of Pisa (Central Italy), were examined to detect DNA of Anaplasma phagocytophilum, Ehrlichia canis, Coxiella burnetii, Francisella tularensis, Hepatozoon canis and Babesia sp./Theileria sp. DNA of vector-borne pathogens was detected in 120 (78.43%; 95% CI: 71.06-84.66%) foxes. Specifically, 75 (49%; 95% CI: 40.86-57.22%) animals scored PCR-positive per H. canis, 68 (44.44%; 95% CI: 36.42-52.69%) for E. canis, 35 (22.88%; 95% CI: 16...
May 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28502276/characterization-of-tabanid-flies-diptera-tabanidae-in-south-africa-and-zambia-and-detection-of-protozoan-parasites-they-are-harbouring
#11
Moeti O Taioe, Makhosazana Y Motloang, Boniface Namangala, Amos Chota, Nthatisi I Molefe, Simon P Musinguzi, Keisuke Suganuma, Polly Hayes, Toi J Tsilo, John Chainey, Noboru Inoue, Oriel M M Thekisoe
Tabanids are haematophagous flies feeding on livestock and wildlife. In the absence of information on the relationship of tabanid flies and protozoan parasites in South Africa and Zambia, the current study was aimed at characterizing tabanid flies collected in these two countries as well as detecting protozoan parasites they are harbouring. A total of 527 tabanid flies were collected whereby 70·2% were from South Africa and 29·8% were from Zambia. Morphological analysis revealed a total of five different genera collected from the sampled areas namely: Ancala, Atylotus, Haematopota, Philoliche and Tabanus...
May 15, 2017: Parasitology
https://www.readbyqxmd.com/read/28501503/detection-and-molecular-characterization-of-babesia-theileria-and-hepatozoon-species-in-hard-ticks-collected-from-kagoshima-the-southern-region-in-japan
#12
Tatsunori Masatani, Kei Hayashi, Masako Andoh, Morihiro Tateno, Yasuyuki Endo, Masahito Asada, Kodai Kusakisako, Tetsuya Tanaka, Mutsuyo Gokuden, Nodoka Hozumi, Fumiko Nakadohzono, Tomohide Matsuo
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southernmost region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2...
March 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28499722/babesia-genotypes-in-haemaphysalis-concinna-collected-from-birds-in-hungary-reflect-phylogeographic-connections-with-siberia-and-the-far-east
#13
Barbara Flaisz, Kinga M Sulyok, Dávid Kováts, Jenő Kontschán, Tibor Csörgő, Ármin Csipak, Miklós Gyuranecz, Sándor Hornok
Haemaphysalis concinna is the second most common tick species attaching to birds in Hungary. Recently, Babesia genotypes, found in Siberia and the Far East, have been detected in this tick species collected from the vegetation in Hungary and Slovakia. The aim of this study was to molecularly investigate if these piroplasms also occur in H. concinna carried by migratory birds, which might explain their occurrence in the western Palaearctic. During a 2-year period, 321 H. concinna larvae and nymphs were collected from 121 passerine birds (of 19 species) in Hungary...
April 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28499721/efficacy-of-south-african-babesia-bovis-vaccine-against-field-isolates
#14
P C Troskie, A A Latif, B J Mans, M P Combrink
A high-passage Babesia bovis vaccine containing only one genotype population was, although protective, inferior compared to the immunity afforded by a lower passage of the same strain containing two populations. The 24 times serially passaged South African B. bovis S vaccine strain contain only a single parasite population (Bv80 allele A 558bp). Forty-four field isolates sampled were all found different with regard to the number and composition of the parasite populations present in each isolate. The extensive genotypic diversity in South Africa and the limited genotypic diversity observed in the S24 vaccine, raised the question on its ability to protect against such diverse populations...
May 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28497743/seroprevalence-of-theileria-equi-and-babesia-caballi-in-horses-in-spain
#15
Maria Guadalupe Montes Cortés, José Luis Fernández-García, Miguel Ángel Habela Martínez-Estéllez
Equine piroplasmoses are enzootic parasitic diseases distributed worldwide with high incidence in tropical and subtropical regions. In Spain, there is insufficient epidemiological data about equine piroplasmoses. The main aim of the present study was therefore to estimate the prevalence of Theileria equi and Babesia caballi in five regions and obtain information about the risk factors. This study was conducted in the central and south-western regions of Spain, using indirect fluorescence antibody testing (IFAT) in 3,100 sera samples from apparently healthy horses of different ages, breeds, coat colours, genders and geographical locations...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28484147/the-presence-of-tick-borne-diseases-in-domestic-dogs-and-cats-living-on-iriomote-jima-and-tsushima-islands
#16
Mao Jikuya, Morihiro Tateno, Masashi Takahashi, Yasuyuki Endo
The Iriomote cat and Tsushima leopard cat are endangered wildcats in Japan and inhabit only Iriomote-jima and Tsushima islands, respectively. Domestic dogs and cats living on Iriomote-jima and Tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. Pathogen-derived DNA in blood samples was detected by polymerase chain reaction. Babesia gibsoni was detected in dogs of Iriomote-jima, and Hepatozoon felis and hemoplasmas were detected in domestic cats of Tsushima...
May 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28464956/geno-and-phenotypic-characteristics-of-a-transfected-babesia-bovis-6-cys-e-knockout-clonal-line
#17
Heba F Alzan, Marta G Silva, William C Davis, David R Herndon, David A Schneider, Carlos E Suarez
BACKGROUND: Babesia bovis is an intra-erythrocytic tick-transmitted apicomplexan protozoan parasite. It has a complex lifestyle including asexual replication in the mammalian host and sexual replication occurring in the midgut of host tick vector, typically, Rhipicephalus microplus. Previous evidence showed that certain B. bovis genes, including members of 6-Cys gene family, are differentially expressed during tick and mammalian stages of the parasite's life cycle. Moreover, the 6-Cys E gene is differentially expressed in the T3Bo strain of B...
May 2, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28455331/reverse-line-blot-based-detection-approaches-of-microbial-pathogens-in-ixodes-ricinus-ticks-collected-in-austria-and-impact-of-the-chosen-method
#18
Anna-Margarita Schötta, Michiel Wijnveld, Hannes Stockinger, Gerold Stanek
Ticks transmit a large number of pathogens capable of causing human disease. In this study, the PCR-reverse line blot (RLB) method was used to screen for pathogens in a total of 554 Ixodes ricinus ticks collected from all provinces of Austria. These pathogens belong to the genera Borrelia, Rickettsiae, Anaplasma/(Neo-)Ehrlichia, Babesia, and CoxiellaThe pathogens with the highest detected prevalence were spirochetes of the Borrelia burgdorferi sensu lato complex, in 142 ticks (25.6%). B. afzelii (80/142) was the most frequently detected species, followed by B...
April 28, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28453188/description-of-15-dna-positive-and-antibody-negative-window-period-blood-donations-identified-during-prospective-screening-for-babesia-microti
#19
Erin D Moritz, Mary Ellen Hewins, Victor P Berardi, Roger Y Dodd, Susan L Stramer
BACKGROUND: Blood donation screening detecting only antibodies fails to identify donors in the earliest stage of infection, before a detectable immunologic response, that is, the "window period" (WP). We present data on WP donations identified during prospective screening for Babesia microti, a transfusion-transmissible parasite of increasing concern in the United States. STUDY DESIGN AND METHODS: Blood donations collected in Connecticut, Massachusetts, Minnesota, and Wisconsin were screened using polymerase chain reaction (PCR) and arrayed fluorescence immunoassay (AFIA) to detect B...
April 28, 2017: Transfusion
https://www.readbyqxmd.com/read/28449722/prevalence-of-tick-borne-haemoparasites-in-small-ruminants-in-turkey-and-diagnostic-sensitivity-of-single-pcr-and-rlb
#20
Huseyin Bilgin Bilgic, Serkan Bakırcı, Onur Kose, Ahmet Hakan Unlu, Selin Hacılarlıoglu, Hasan Eren, William Weir, Tulin Karagenc
BACKGROUND: Tick-borne haemoparasitic diseases (TBHDs), caused by Theileria, Babesia, Anaplasma and Ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. Many of these diseases threaten livestock production and some also represent a concern to human public health. The primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) in Turkey...
April 27, 2017: Parasites & Vectors
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