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https://www.readbyqxmd.com/read/28508164/viability-of-strongyloides-venezuelensis-eggs-and-larvae-in-vermiculite-containing-the-fungus-duddingtonia-flagrans
#1
Laryssa Pinheiro Costa Silva, Carolina Magri Ferraz, Anderson Rocha Aguiar, Jackson Victor Araújo, Steveen Rios Ribeiro, Débora Goldner Rossi, Luanderson Queiroz Mendes, Fausto Edmundo Lima Pereira, Narcisa Imaculada Brant Moreira, Fabio Ribeiro Braga
Strongyloidiasis is the most clinically important disease among the infections caused by geohelminths, seeing that this parasite can cause autoinfection. The use of nematophagous fungi like Duddingtonia flagrans, that have predation action on eggs and infecciososas forms of helminths, emerges as an alternative method for environmental control. For this reason, analyzing the viability of larvae and eggs of Strongyloides venezuelensis and the action of Duddingtonia flagrans AC001 in vermiculite, as well as the action of the nematophagous fungi in different growth stages, is important to elaborate and define the best culture conditions that favor the activity of the fungus...
May 15, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28506307/intestinal-parasitic-infections-and-its-association-with-undernutrition-and-cd4-t-cell-levels-among-hiv-aids-patients-on-haart-in-butajira-ethiopia
#2
Dereje Gedle, Gemechu Kumera, Tewodros Eshete, Kasahun Ketema, Haweni Adugna, Fetuma Feyera
BACKGROUND: Intestinal parasitic infections and HIV/AIDS have been the major public health problems and remain a vital cause of morbidity and mortality in developing countries. Both problems are linked in a vicious cycle. The magnitude of intestinal parasites was prevalent among people living with HIV/AIDS even in the HAART era. However, the pertinent risk factors associated with intestinal parasites among HIV/AIDS patients were not well investigated in Ethiopia particularly at Butajira town...
May 15, 2017: Journal of Health, Population, and Nutrition
https://www.readbyqxmd.com/read/28505198/strongyloides-seroprevalence-before-and-after-an-ivermectin-mass-drug-administration-in-a-remote-australian-aboriginal-community
#3
Therese M Kearns, Bart J Currie, Allen C Cheng, James McCarthy, Jonathan R Carapetis, Deborah C Holt, Wendy Page, Jennifer Shield, Roslyn Gundjirryirr, Eddie Mulholland, Linda Ward, Ross M Andrews
BACKGROUND: Strongyloides seroprevalence is hyper-endemic in many Australian Aboriginal and Torres Strait Islander communities, ranging from 35-60%. We report the impact on Strongyloides seroprevalence after two oral ivermectin mass drug administrations (MDAs) delivered 12 months apart in a remote Australian Aboriginal community. METHODS: Utilizing a before and after study design, we measured Strongyloides seroprevalence through population census with sequential MDAs at baseline and month 12...
May 15, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28500375/first-molecular-identification-and-genetic-diversity-of-strongyloides-stercoralis-and-strongyloides-fuelleborni-in-human-communities-having-contact-with-long-tailed-macaques-in-thailand
#4
Tongjit Thanchomnang, Pewpan M Intapan, Oranuch Sanpool, Rutchanee Rodpai, Somjintana Tourtip, Sujitra Yahom, Jitsuda Kullawat, Prayong Radomyos, Chalida Thammasiri, Wanchai Maleewong
The parasitic nematodes, Strongyloides stercoralis and Strongyloides fuelleborni, can infect humans and non-human primates. We amplified and sequenced a portion of the 18S ribosomal RNA gene (rRNA) and of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of Strongyloides from humans in the study area in Thailand, where people have frequent contact with long-tailed macaques. Fresh stool samples were obtained from 213 people and were examined using the agar plate culture method. The overall prevalence of Strongyloides infection was 8...
May 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28495192/effect-of-creep-fed-supplement-on-the-susceptibility-of-pasture-grazed-suckling-lambs-to-gastrointestinal-helminths
#5
Gleice Kelli Ayardes de Melo, Camila Celeste Brandão Ferreira Ítavo, Kedma Leonora Silva Monteiro, Jonilson Araújo da Silva, Pâmila Carolini Gonçalves da Silva, Luís Carlos Vinhas Ítavo, Dyego Gonçalves Lino Borges, Fernando de Almeida Borges
This study evaluated the effect of creep feeding a protein supplement on the susceptibility of suckling lambs to infection with gastrointestinal helminths. Male and female lambs were grazed on Brachiaria spp. pastures next to their mothers. Animals were allocated to one of two treatments: creep feeding (261g/d) and control (no supplementation). The trial period was the suckling of lambs during two years of study: May-October 2013 and March-July 2014. Supplementary creep feeding of lambs improved animal performance (P<0...
May 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28493000/serological-and-molecular-tests-for-the-diagnosis-of-strongyloides-stercoralis-infection-in-dogs
#6
Dora Buonfrate, Paola Paradies, Fabrizio Iarussi, Fabio Formenti, Francesca Perandin, Domenico Otranto, Zeno Bisoffi
Strongyloides stercoralis can cause severe infection both in humans and dogs. Coproparasitological examination has low sensitivity for the diagnosis of this parasite; hence, different diagnostic techniques have been implemented. However, serology and molecular methods have been assessed almost exclusively in humans. In this study, two serologic assays and a real-time PCR (RT-PCR), routinely used for the diagnosis of strongyloidiasis in humans, have been tested for the diagnosis in dogs. Five dogs living in the same kennel in Bari, southern Italy, were diagnosed with S...
May 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28483729/presence-of-parasites-in-environmental-waters-in-samsun-and-its-districts
#7
Ülkü Karaman, Zeynep Kolören, Onuralp Seferoğlu, Emine Ayaz, Elif Demirel
OBJECTIVE: The aim of this study was to detect the presence of parasites in environmental waters in Samsun and its districts. METHODS: At the center of Samsun, 13 stations were determined. The research was performed between March 2012 and February 2013, and every month, water samples were collected on the dates stated. The samples were stained with Kinyoun acid-fast, modified trichrome, and trichrome dyes after examining with the direct bond. The preparations were evaluated in terms of parasitologic under a light microscope...
March 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28476340/first-report-of-human-intestinal-sarcocystosis-in-cambodia
#8
Virak Khieu, Hanspeter Marti, Saomony Chhay, Meng Chuor Char, Sinuon Muth, Peter Odermatt
Human intestinal sarcocystosis (HIS), caused by Sarcocystis species, is acquired by eating undercooked meat from sarcocyst-containing cattle (S. hominis, S. heydorni) and pigs (S. suihominis). We report on the detection of human intestinal Sarcocystis infections in a cross-sectional survey of Strongyloides stercoralis in early 2014, in Rovieng District, Preah Vihear Province, northern Cambodia. Among 1081 participants, 108 (10.0%) were diagnosed with Sarcocystis spp. oocysts in stool samples. Males had a significantly higher risk of infection than females (OR: 1...
May 2, 2017: Parasitology International
https://www.readbyqxmd.com/read/28469498/twenty-five-years-of-chronic-strongyloidiasis-in-an-immigrant
#9
Kalambay N Junior, Rehana Zaman, Mohammed H Zaman, Taufiq Zaman
Chronic strongyloidiasis is an infection of the tropical regions, caused by the nematode Strongyloides stercoralis. In the United States, patients are typically immigrants. The very long asymptomatic phase followed by the clinical presentation of the disease mimics asthma, chronic obstructive pulmonary disease (COPD), or inflammatory bowel disease (IBD) as in this case report. The inconsistency of eosinophilia and the low sensitivity of microscopic stool examination make chronic strongyloidiasis a disease that is frequently misdiagnosed in the United States...
2017: Clinical Medicine Insights. Case Reports
https://www.readbyqxmd.com/read/28464844/pulmonary-strongyloidiasis-assessment-between-manifestation-and-radiological-findings-in-16-severe-strongyloidiasis-cases
#10
Daijiro Nabeya, Shusaku Haranaga, Gretchen Lynn Parrott, Takeshi Kinjo, Saifun Nahar, Teruhisa Tanaka, Tetsuo Hirata, Akira Hokama, Masao Tateyama, Jiro Fujita
BACKGROUND: Strongyloidiasis is a chronic parasitic infection caused by Strongyloides stercoralis. Severe cases such as, hyperinfection syndrome (HS) and disseminated strongyloidiasis (DS), can involve pulmonary manifestations. These manifestations frequently aid the diagnosis of strongyloidiasis. Here, we present the pulmonary manifestations and radiological findings of severe strongyloidiasis. METHODS: From January 2004 to December 2014, all patients diagnosed with severe strongyloidiasis at the University of the Ryukyus Hospital or affiliated hospitals in Okinawa, Japan, were included in this retrospective study...
May 2, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28462089/helminth-parasites-of-howler-and-spider-monkeys-in-mexico-insights-into-molecular-diagnostic-methods-and-their-importance-for-zoonotic-diseases-and-host-conservation
#11
Brenda Solórzano-García, Gerardo Pérez-Ponce de León
The majority of the parasite assessments of New World primates have been conducted through the identification of the eggs found in faeces, though many species of parasites have very similar eggs, leaving uncertainty in the diagnosis. Here, we present the results of a parasite survey of the three species of primates distributed in Mexico, combining non-invasive sampling with molecular techniques via DNA extraction of the eggs found in the faeces. Mitochondrial and ribosomal DNA were employed for species identification and Bayesian phylogenetic analysis...
August 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28459817/modulation-of-mycobacterium-tuberculosis-specific-humoral-immune-responses-is-associated-with-strongyloides-stercoralis-co-infection
#12
Rajamanickam Anuradha, Saravanan Munisankar, Yukti Bhootra, Chandrakumar Dolla, Paul Kumaran, Thomas B Nutman, Subash Babu
BACKGROUND / OBJECTIVES: Helminth infections are known to influence T cell responses in latent tuberculosis (LTBI). Whether helminth infections also modulate B cell responses in helminth-tuberculosis co-infection is not known. METHODS: We assessed Mycobacterium tuberculosis (Mtb)-antigen specific IgM and IgG levels, circulating levels of the B cell growth factors, BAFF and APRIL and the absolute numbers of the various B cell subsets in individuals with LTBI, LTBI with coincident Strongyloides stercoralis (Ss) infection (LTBI/Ss) and in those with Ss infection alone (Ss)...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28459014/detection-of-cryptosporidium-in-stool-samples-of-immunocompromised-patients
#13
K Vanathy, Subhash Chandra Parija, Jharna Mandal, Abdoul Hamide, Sriram Krishnamurthy
INTRODUCTION: Cryptosporidium species is the most common opportunistic enteric parasite encountered in the immunocompromised patients. Considering the need to diagnose them early relies mostly on rapid tests such as antigen detection by immunochromatographic test (ICT), ELISA, and microscopy. However, the sensitivity and specificity varies with different methods and different kits used. This study was conducted to determine the intestinal parasitic profile in immunocompromised patients and to assess the diagnostic accuracy of the ICT using ImmunoCard STAT kit in detecting Cryptosporidium spp...
January 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/28455628/identification-of-antigenic-proteins-in-strongyloides-stercoralis-by-proteomic-analysis
#14
Rutchanee Rodpai, Pewpan M Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Porntip Laummaunwai, Chaisiri Wongkham, Tonkla Insawang, Wanchai Maleewong
Strongyloides stercoralis is an intestinal helminth that infects people worldwide. Hyperinfection or disseminated human strongyloidiasis can involve vital organs, leading to lethal outcomes. We analyzed immunoproteomics of antigenic spots, derived from S. stercoralis third-stage larvae and reacted with human strongyloidiasis sera, by two-dimensional gel electrophoresis and immunoblotting. Of 26 excised antigenic spots analyzed by liquid chromatography-electrospray ionization-tandem mass spectrometry, 20 proteins were identified...
April 29, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28451440/microbiological-screenings-for-infection-control-in-unaccompanied-minor-refugees-the-german-armed-forces-medical-service-s-experience
#15
Winfried Maaßen, Dorothea Wiemer, Claudia Frey, Christina Kreuzberg, Egbert Tannich, Rebecca Hinz, Andreas Wille, Andreas Fritsch, Ralf Matthias Hagen, Hagen Frickmann
BACKGROUND: The German Military Medical Service contributed to the medical screening of unaccompanied minor refugees (UMRs) coming to Germany in 2014 and 2015. In this study, a broad range of diagnostic procedures was applied to identify microorganisms with clinical or public health significance. Previously, those tests had only been used to screen soldiers returning from tropical deployments. This instance is the first time the approach has been studied in a humanitarian context. METHODS: The offered screenings included blood cell counts, hepatitis B serology and microscopy of the stool to look for protozoa and worm eggs as well as PCR from stool samples targeting pathogenic bacteria, protozoa and helminths...
2017: Military Medical Research
https://www.readbyqxmd.com/read/28443982/enzyme-linked-immunosorbent-assay-for-diagnosis-of-amphimerus-spp-liver-fluke-infection-in-humans
#16
William Cevallos, Manuel Calvopiña, Victoria Nipáz, Belén Vicente-Santiago, Julio López-Albán, Pedro Fernández-Soto, Ángel Guevara, Antonio Muro
BACKGROUND: Amphimerus spp. is a liver fluke that infects humans and domestic animals. It is highly prevalent in some Ecuadorian communities. Currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. More sensitive methods are needed for diagnostic testing. OBJECTIVE: The main objective of this work was to develop an enzyme-linked immunosorbent assay (ELISA) using crude antigens from Amphimerus spp. adult worms to detect anti-Amphimerus IgG in human sera...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28414672/a-strong-and-fortuitous-case-of-dyspnea
#17
R Hammer, M Sciaudone
CASE: A 48 year-old man with no past medical history was sent to our emergency department (ED); from a primary care clinic for hypertensive urgency of 200/130. The man reported an intermittent non-productive cough of approximately one year's duration and worsening dyspnea on exertion and orthopnea over the last month with lower extremity swelling. Of note, he emigrated from Honduras twenty years ago. Blood pressure normalized with administration of Lasix in the ED. Physical exam revealed rales in lung bases bilaterally, jugular venous distension, lower extremity pitting edema with serpiginous patches of erythema and excoriation, and a cardiac gallop...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28380118/in-vitro-efficacy-of-latex-and-purified-papain-from-carica-papaya-against-strongyloides-venezuelensis-eggs-and-larvae
#18
Dayane Moraes, Marcelo Arantes Levenhagen, Julia Maria Costa-Cruz, Antônio Paulino da Costa, Rosângela Maria Rodrigues
Latex from Carica papaya is rich in bioactive compounds, especially papain, which may help to control parasitic diseases. This study evaluated the efficacy of latex from C. papaya and purified papain against Strongyloides venezuelensis. The Egg Hatching Test (EHT) and the Larval Motility Test (LMT) using fresh and frozen latex (250mg/mL), lyophilized latex (34mg/mL), and purified papain (2.8 mg/mL) were performed. Albendazole (0.025 mg/mL) and ivermectin (316 ppm) were used as positive controls. EHT and LMT were carried out through the incubation of each solution with S...
April 3, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28377220/estimating-occurrence-of-strongyloides-stercoralis-in-the-caribbean-island-countries-implications-for-monitoring-and-control
#19
Jennifer K Ketzis, Anne Conan
BACKGROUND: Few data are available for the prevalence of Strongyloides stercoralis in the Caribbean region. This frequently under diagnosed soil-transmitted helminth (STH) can result in long-term low intensity chronic infections that are asymptomatic or can cause varied intestinal disturbances. With autoinfections, infections lasting over 60 years can occur and hyperinfections lead to high morbidity and mortality. METHODOLOGY/PRINCIPAL FINDINGS: Historical literature was searched to determine the prevalence of S...
April 1, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28377165/a-case-of-strongyloides-hyperinfection-syndrome-in-the-setting-of-persistent-eosinophilia-but-negative-serology
#20
Stephen Osiro, Camille Hamula, Allison Glaser, Meenakshi Rana, Dallas Dunn
Strongyloides stercoralis is a unique intestinal nematode with the ability to replicate and complete its life cycle without leaving the host. We report a fatal case of Strongyloides hyperinfection syndrome in a patient who had persistent eosinophilia for several years but negative Strongyloides serology. Our case suggests that ELISA serologies cannot solely be relied upon to diagnose Strongyloides stercoralis infection; history and clinical judgment remain crucial to this diagnosis.
March 14, 2017: Diagnostic Microbiology and Infectious Disease
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