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https://www.readbyqxmd.com/read/28443982/enzyme-linked-immunosorbent-assay-for-diagnosis-of-amphimerus-spp-liver-fluke-infection-in-humans
#1
William Cevallos, Manuel Calvopiña, Victoria Nipáz, Belén Vicente-Santiago, Julio López-Albán, Pedro Fernández-Soto, Ángel Guevara, Antonio Muro
BACKGROUND: Amphimerus spp. is a liver fluke that infects humans and domestic animals. It is highly prevalent in some Ecuadorian communities. Currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. More sensitive methods are needed for diagnostic testing. OBJECTIVE: The main objective of this work was to develop an enzyme-linked immunosorbent assay (ELISA) using crude antigens from Amphimerus spp. adult worms to detect anti-Amphimerus IgG in human sera...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28414672/a-strong-and-fortuitous-case-of-dyspnea
#2
R Hammer, M Sciaudone
CASE: A 48 year-old man with no past medical history was sent to our emergency department (ED); from a primary care clinic for hypertensive urgency of 200/130. The man reported an intermittent non-productive cough of approximately one year's duration and worsening dyspnea on exertion and orthopnea over the last month with lower extremity swelling. Of note, he emigrated from Honduras twenty years ago. Blood pressure normalized with administration of Lasix in the ED. Physical exam revealed rales in lung bases bilaterally, jugular venous distension, lower extremity pitting edema with serpiginous patches of erythema and excoriation, and a cardiac gallop...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28380118/in-vitro-efficacy-of-latex-and-purified-papain-from-carica-papaya-against-strongyloides-venezuelensis-eggs-and-larvae
#3
Dayane Moraes, Marcelo Arantes Levenhagen, Julia Maria Costa-Cruz, Antônio Paulino da Costa, Rosângela Maria Rodrigues
Latex from Carica papaya is rich in bioactive compounds, especially papain, which may help to control parasitic diseases. This study evaluated the efficacy of latex from C. papaya and purified papain against Strongyloides venezuelensis. The Egg Hatching Test (EHT) and the Larval Motility Test (LMT) using fresh and frozen latex (250mg/mL), lyophilized latex (34mg/mL), and purified papain (2.8 mg/mL) were performed. Albendazole (0.025 mg/mL) and ivermectin (316 ppm) were used as positive controls. EHT and LMT were carried out through the incubation of each solution with S...
April 3, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28377220/estimating-occurrence-of-strongyloides-stercoralis-in-the-caribbean-island-countries-implications-for-monitoring-and-control
#4
Jennifer K Ketzis, Anne Conan
BACKGROUND: Few data are available for the prevalence of Strongyloides stercoralis in the Caribbean region. This frequently under diagnosed soil-transmitted helminth (STH) can result in long-term low intensity chronic infections that are asymptomatic or can cause varied intestinal disturbances. With autoinfections, infections lasting over 60 years can occur and hyperinfections lead to high morbidity and mortality. METHODOLOGY/PRINCIPAL FINDINGS: Historical literature was searched to determine the prevalence of S...
April 1, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28377165/a-case-of-strongyloides-hyperinfection-syndrome-in-the-setting-of-persistent-eosinophilia-but-negative-serology
#5
Stephen Osiro, Camille Hamula, Allison Glaser, Meenakshi Rana, Dallas Dunn
Strongyloides stercoralis is a unique intestinal nematode with the ability to replicate and complete its life cycle without leaving the host. We report a fatal case of Strongyloides hyperinfection syndrome in a patient who had persistent eosinophilia for several years but negative Strongyloides serology. Our case suggests that ELISA serologies cannot solely be relied upon to diagnose Strongyloides stercoralis infection; history and clinical judgment remain crucial to this diagnosis.
March 14, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28370833/infectious-complications-in-indigenous-renal-transplant-recipients-in-western-australia
#6
Peter Boan, Ramyasuda Swaminathan, Ashley Irish
BACKGROUND: Infectious complications remain a significant risk following renal transplantation. AIMS: To examine the burden and pattern of infection following renal transplantation in Aboriginal and Torres Strait Islanders (ATSI) compared to non-ATSI. METHODS: Retrospective cohort study of 141 consecutive adult renal transplant recipients in Western Australia between 2005 and 2011. We determined baseline serological status for relevant organisms, the number of patients with specific infections and infectious admission in the first year post transplantation and the rate of infectious death during follow up...
April 3, 2017: Internal Medicine Journal
https://www.readbyqxmd.com/read/28369530/efficacy-of-moxidectin-versus-ivermectin-against-strongyloides-stercoralis-infections-a-randomized-controlled-non-inferiority-trial
#7
Beatrice Barda, Somphou Sayasone, Khampheng Phongluxa, Syda Xayavong, Khonsavanh Keoduangsy, Peter Odermatt, Maxim Puchkov, Jörg Huwyler, Jan Hattendorf, Jennifer Keiser
Background: Infections with Strongyloides stercoralis are of considerable public health relevance. Moxidectin, a well-established drug in veterinary medicine under consideration for regulatory submission for the treatment of onchocerciasis, might serve as alternative to the most widely used ivermectin. Methods: We conducted an exploratory, randomized, single-blind trial to evaluate the efficacy and safety of moxidectin (8 mg) versus ivermectin (200 μg/kg) against S...
March 25, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28366929/strongyloides-stercoralis-infection-among-human-immunodeficiency-virus-hiv-infected-patients-in-the-united-states-of-america-a-case-report-and-review-of-literature
#8
Laia Jimena Vazquez Guillamet, Zane Saul, Goran Miljkovich, Gabriel Alejandro Vilchez, Nikolai Mendonca, Venkata Gourineni, Nicholas Lillo, Marguerite Pinto, Aurengzaib Baig, Louie Mar Gangcuangco
BACKGROUND Strongyloides stercoralis infection is endemic in subtropical and tropical regions but is reported rather sporadically in temperate countries. In the USA, the highest rates of infection are from the southeastern states, predominantly among immigrants. There is paucity of case reports on S. stercoralis infection among HIV-infected patients who were born and raised in the USA. CASE REPORT A 61-year-old male with known HIV infection (CD4 count: 235 cells/uL, undetectable HIV RNA, on antiretroviral therapy) presented with a 3-month history of diarrhea...
April 3, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28347664/venestatin-a-ca-binding-protein-from-the-parasitic-nematode-strongyloides-venezuelensis-is-involved-in-the-larval-migration-process
#9
Daigo Tsubokawa, Takeshi Hatta, Taisei Kikuchi, Hiroki Maeda, Fusako Mikami, M Abdul Alim, Haruhiko Maruyama, Naotoshi Tsuji
The secretory EF-hand Ca(++)-binding proteins act as calcium signaling molecules for control of cell functions, but those proteins from parasitic helminths are poorly understood. Here, we have identified and characterized an EF-hand Ca(++)-binding protein from the rodent nematode, Strongyloides venezuelensis, termed 'venestatin', which is highly conserved in Strongyloides spp. Canonical two EF-hand domains and a signal peptide are present in venestatin. A gel mobility shift assay and Ruthenium red staining indicated that the recombinant venestatin possesses binding ability with Ca(++) ions...
March 24, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28346396/a-pcr-based-molecular-detection-of-strongyloides-stercoralisin-human-stool-samples-from-tabriz-city-iran
#10
Reza Ghasemikhah, Mohammad Amin Tabatabaiefar, Seyed Ali Shariatzadeh, Abbas Shahbazi, Teymour Hazratian
Strongyloides stercoralis is a nematode causing serious infections in immunocompromised patients. In chronically infected patients, the low parasitic content as well as the resemblance of the larvae to several other species make diagnosis basedonmorphology difficult. In the present study, a PCR-based method targeting the internal transcribed sequence 2 (ITS2) of the rDNA region was examined for the molecular detection of S. stercoralis infection from the stool samples. A total of 1800 patients were included...
March 27, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28345791/trichoscopic-diagnosis-of-cutaneous-pelodera-strongyloides-infestation-in-a-dog
#11
Rareş G M Căpitan, Chiara Noli
BACKGROUND: Pelodera strongyloides infestation has rarely been reported in dogs. It is a saprophytic free-living nematode, found in moist decaying organic matter, whose L3 larval stages occasionally can invade the skin of dogs and humans. Larvae penetrate the follicular infundibula causing an erythematous and pruritic dermatitis. The disease is usually diagnosed by deep skin scraping or histopathology, with large numbers of parasites readily identified in hair follicles. CASE REPORT: Pelodera strongyloides dermatitis was diagnosed in a six-year-old mixed breed dog presenting with alopecia, erythema and severe pruritus on the ventrum and limbs...
March 27, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28338804/duplications-and-positive-selection-drive-the-evolution-of-parasitism-associated-gene-families-in-the-nematode-strongyloides-papillosus
#12
Praveen Baskaran, Tegegn G Jaleta, Adrian Streit, Christian Rödelsperger
Gene duplication is a major mechanism playing a role in the evolution of phenotypic complexity and in the generation of novel traits. By comparing parasitic and nonparasitic nematodes, a recent study found that the evolution of parasitism in Strongyloididae is associated with a large expansion in the Astacin and CAP gene families.To gain novel insights into the developmental processes in the sheep parasite Strongyloides papillosus, we sequenced transcriptomes of different developmental stages and sexes. Overall, we found that the majority of genes are developmentally regulated and have one-to-one orthologs in the diverged S...
March 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28336269/epidemiology-of-polyparasitism-with-taenia-solium-schistosomes-and-soil-transmitted-helminths-in-the-co-endemic-village-of-malanga-democratic-republic-of-congo
#13
Joule Madinga, Katja Polman, Kirezi Kanobana, Lisette van Lieshout, Eric Brienen, Nicolas Praet, Constantin Kabwe, Sarah Gabriël, Pierre Dorny, Pascal Lutumba, Niko Speybroeck
Helminth co-infections are common in sub-Saharan Africa. However, little is known about the distribution and determinants of co-infections with Taenia solium taeniasis/cysticercosis. Building on a previous community-based study on human cysticercosis in Malanga village, we investigated co-infections with Taenia solium, soil-transmitted helminths (STHs) and Schistosoma spp and associated risk factors in a random subsample of 330 participants. Real time PCR assays were used to detect DNA of soil-transmitted helminths (STHs), T...
March 20, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28331839/enterococcal-meningitis-in-association-with-strongyloides-hyperinfection-syndrome
#14
Kalpesh S Sukhwani, Nitin Bansal, Mamta Soni, Anand Ramamurthy, Ram Gopalakrishnan
INTRODUCTION: Strongyloidiasis can cause hyperinfection or disseminated infection in an immunocompromised host, and is an important factor linked to enterococcal bacteremia and meningitis. CASE REPORTS: We report two cases highlighting the importance of suspecting Strongyloides hyperinfection syndrome in patients with enterococcal meningitis. CONCLUSION: Our cases highlight the importance of suspecting Strongyloides hyperinfection syndrome in cases of community acquired enterococcal bacteremia and meningitis...
March 2017: Germs
https://www.readbyqxmd.com/read/28331018/strongyloides-hyperinfection-syndrome-in-an-immunocompetent-host-resulting-in-bandemia-and-death
#15
Aung Myint, Courtney Chapman, Isabel Almira-Suarez, Nupur Mehta
Strongyloides stercoralis infection is usually asymptomatic but can result in a hyperinfection syndrome, most commonly triggered by acquired or iatrogenic immunosuppression. Here, we present a case of a man aged 60 years originally from a strongyloides endemic area with a medical history of alcohol abuse who presents with strongyloides hyperinfection syndrome (SHS) complicated by partial small bowel obstruction, pulmonary haemorrhage, large bandemia without eosinophilia and cardiac arrest resulting in death...
March 22, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28316848/strongyloides-hyperinfection-in-a-renal-transplant-patient-always-be-on-the-lookout
#16
Murtaza Mazhar, Ijlal Akbar Ali, Nelson Iván Agudelo Higuita
We present a case of a 71-year-old Vietnamese man with chronic kidney disease secondary to adult polycystic kidney disease. He had been a prisoner of war before undergoing a successful cadaveric renal transplant in the United States. He presented to clinic one year after the transplant with gross hematuria, productive cough, intermittent chills, and weight loss. Long standing peripheral eosinophilia of 600-1200/μL triggered further evaluation. A wet mount of stool revealed Strongyloides stercoralis larvae...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28279703/limitations-to-the-adoption-of-a-standardized-strongyloides-stercoralis-diagnostic-method-case-study-in-the-caribbean
#17
Jennifer K Ketzis
BACKGROUND: Strongyloides stercoralis is frequently under-diagnosed due to the low sensitivity of common faecal diagnostic methods used in clinical laboratories. This leads to a belief that prevalence is low and that S. stercoralis is not an important soil-transmitted helminth (STH). S. stercoralis diagnostic methods with higher sensitivity are available but often not used. Reasons for their lack of use need to be identified and addressed in order to increase awareness of this neglected parasite...
March 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28273080/geospatial-distribution-of-intestinal-parasitic-infections-in-rio-de-janeiro-brazil-and-its-association-with-social-determinants
#18
Clarissa Perez Faria, Graziela Maria Zanini, Gisele Silva Dias, Sidnei da Silva, Marcelo Bessa de Freitas, Ricardo Almendra, Paula Santana, Maria do Céu Sousa
BACKGROUND: Intestinal parasitic infections remain among the most common infectious diseases worldwide. This study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of Rio de Janeiro, considering demographic, socio-economic, and epidemiological contextual factors. METHODS/PRINCIPAL FINDINGS: The cross-section survey was conducted among individuals attending the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, RJ) during the period from April 2012 to February 2015...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28264908/differences-in-the-importance-of-mast-cells-basophils-ige-and-igg-versus-cd4-t-cells-and-ilc2-cells-in-primary-and-secondary-immunity-to-strongyloides-venezuelensis
#19
Kaori Mukai, Hajime Karasuyama, Kenji Kabashima, Masato Kubo, Stephen J Galli
There is evidence that mast cells, basophils and IgE can contribute to immune responses to parasites, however, the relative importance of these effector elements in parasite immunity is not fully understood. Previous work in Il3-deficient and c-kit mutant Kit(W/W-v) mice indicated that interleukin-3 and c-Kit contribute to expulsion of the intestinal nematode Strongyloides venezuelensis (S.v.) during primary infection. Our findings in mast cell-deficient Kit(W-sh/W-sh) mice and two types of mast cell-deficient mice that have normal c-kit: "Hello Kitty" and MasTRECK mice, confirmed prior work in Kit(W/W-v) mice suggesting that mast cells play an important role in S...
March 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28261873/purpuric-lesion-in-an-immunocompromised-patient-a-presentation-of-disseminated-strongyloidiasis
#20
LETTER
A D da Silva Citrin, J A Guzmán, J Barros, C D Machado-Filho
No abstract text is available yet for this article.
March 6, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
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