keyword
MENU ▼
Read by QxMD icon Read
search

Periodontics

keyword
https://www.readbyqxmd.com/read/27926431/the-oral-microbiome-in-health-and-its-implication-in-oral-and-systemic-diseases
#1
B Sampaio-Maia, I M Caldas, M L Pereira, D Pérez-Mongiovi, R Araujo
The oral microbiome can alter the balance between health and disease, locally and systemically. Within the oral cavity, bacteria, archaea, fungi, protozoa, and viruses may all be found, each having a particular role, but strongly interacting with each other and with the host, in sickness or in health. A description on how colonization occurs and how the oral microbiome dynamically evolves throughout the host's life is given. In this chapter the authors also address oral and nonoral conditions in which oral microorganisms may play a role in the etiology and progression, presenting the up-to-date knowledge on oral dysbiosis as well as the known underlying pathophysiologic mechanisms involving oral microorganisms in each condition...
2016: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/27925695/integrated-biomarker-profiling-of-smokers-with-periodontitis
#2
Radhakrishnan Nagarajan, Mohanad Al-Sabbagh, Dolph Dawson, Jeffrey Ebersole
: In the context of precision medicine, understanding patient-specific variation is an important step in developing targeted and patient-tailored treatment regimens for periodontitis. While several studies have successfully demonstrated the usefulness of molecular expression profiling in conjunction with single classifier systems in discerning distinct disease groups, the majority of these studies do not provide sufficient insights into potential variations within the disease groups. AIM: The goal of the present study is to discern biological response profiles of periodontitis and non-periodontitis smoking subjects using an informed panel of biomarkers across multiple scales (salivary, oral microbiome, pathogens and other markers)...
December 7, 2016: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/27925202/calprotectin-induces-il-6-and-mcp-1-production-via-toll-like-receptor-4-signaling-in-human-gingival-fibroblasts
#3
Yasufumi Nishikawa, Yukari Kajiura, Jung Hwan Lew, Jun-Ichi Kido, Toshihiko Nagata, Koji Naruishi
Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs)...
December 7, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27924938/the-secretome-of-periodontal-ligament-stem-cells-from-ms-patients-protects-against-eae
#4
Thangavelu Soundara Rajan, Sabrina Giacoppo, Francesca Diomede, Patrizia Ballerini, Michele Paolantonio, Marco Marchisio, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani
Manipulation of stem cells or stem cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament stem cells (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients and healthy donors block experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing anti-inflammatory and immunosuppressive effects in spinal cord and spleen, and reverse disease progression by restoring tissue integrity via remyelination in the spinal cord...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924613/characterization-quantification-and-visualization-of-neutrophil-extracellular-traps
#5
Phillipa C White, Ilaria J Chicca, Martin R Ling, Helen J Wright, Paul R Cooper, Mike R Milward, Iain L C Chapple
Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924608/a-method-to-isolate-purify-and-characterize-human-periodontal-ligament-stem-cells
#6
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P Mark Bartold
Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924607/fabrication-and-characterization-of-decellularized-periodontal-ligament-cell-sheet-constructs
#7
Amro Farag, Cédryck Vaquette, Dietmar W Hutmacher, P Mark Bartold, Saso Ivanovski
Decellularized tissue-engineered constructs have the potential to promote regeneration by providing a biomimetic extracellular matrix that directs tissue-specific regeneration when implanted in situ. Recently, the use of cell sheets has shown promising results in promoting periodontal regeneration. Here, we describe the fabrication of decellularized periodontal cell sheets with intact extracellular matrix structural and biological properties. Melt electro spun polycaprolactone (PCL) scaffolds are used as a carrier for the inherently fragile cell sheets, to provide support during the processes of decellularization...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924604/exploring-genome-wide-expression-profiles-using-machine-learning-techniques
#8
Moritz Kebschull, Panos N Papapanou
Although contemporary high-throughput -omics methods produce high-dimensional data, the resulting wealth of information is difficult to assess using traditional statistical procedures. Machine learning methods facilitate the detection of additional patterns, beyond the mere identification of lists of features that differ between groups.Here, we demonstrate the utility of (1) supervised classification algorithms in class validation, and (2) unsupervised clustering in class discovery. We use data from our previous work that described the transcriptional profiles of gingival tissue samples obtained from subjects suffering from chronic or aggressive periodontitis (1) to test whether the two diagnostic entities were also characterized by differences on the molecular level, and (2) to search for a novel, alternative classification of periodontitis based on the tissue transcriptomes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924603/differential-expression-and-functional-analysis-of-high-throughput-omics-data-using-open-source-tools
#9
Moritz Kebschull, Melanie Julia Fittler, Ryan T Demmer, Panos N Papapanou
Today, -omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ, or tissue sample, allow for an unbiased, comprehensive genome-level analysis of complex diseases, offering a large advantage over earlier "candidate" gene or pathway analyses. A primary goal in the analysis of these high-throughput assays is the detection of those features among several thousand that differ between different groups of samples. In the context of oral biology, our group has successfully utilized -omics technology to identify key molecules and pathways in different diagnostic entities of periodontal disease...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924602/genome-wide-analysis-of-periodontal-and-peri-implant-cells-and-tissues
#10
Moritz Kebschull, Claudia Hülsmann, Per Hoffmann, Panos N Papapanou
Omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ or tissue sample, are powerful means of generating comprehensive genome-level data sets on complex diseases. We have systematically assessed the transcriptome, miRNome and methylome of gingival tissues from subjects with different diagnostic entities of periodontal disease, and studied the transcriptome of primary cells ex vivo, or in vitro after infection with periodontal pathogens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924596/methods-to-study-antagonistic-activities-among-oral-bacteria
#11
Fengxia Qi, Jens Kreth
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate with other neighboring species in order to perpetuate in the multispecies community. The human oral cavity is colonized by >700 microbial species known as the indigenous microbiota. This indigenous flora normally maintains an ecological balance through antagonistic as well as mutualistic interspecies interactions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924595/quantitative-analysis-of-periodontal-pathogens-using-real-time-polymerase-chain-reaction-pcr
#12
Mª José Marin, Elena Figuero, David Herrera, Mariano Sanz
The quantitative polymerase chain reaction (qPCR) is a variant of PCR aimed to detect and quantify a targeted DNA molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated PCR product. This chapter presents the detailed procedures for quantification of different periodontal pathogens (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, and Fusobacterium spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924587/qualitative-and-quantitative-proteome-analysis-of-oral-fluids-in-health-and-periodontal-disease-by-mass-spectrometry
#13
Erdjan Salih
The significance of protein identification and characterization by classical protein chemistry approaches is clearly highlighted by our detailed understanding of the biological systems assembled over a time period of almost a century. The advent of state-of-the-art mass spectrometry (MS) with sensitivity, speed, and global protein analysis capacity without individual protein purification has transformed the classical protein chemistry with premise to accelerate discovery. These combined with the ability of the oral fluids such as whole saliva (WS) and gingival crevicular fluid (GCF) to reflect both systemic and locally derived proteins have generated significant interest to characterize these fluids more extensively by MS technology...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27922193/phagocytosis-of-collagen-fibrils-by-fibroblasts-in-vivo-is-independent-of-the-uparap-endo180-receptor
#14
Sara Sprangers, Niels Behrendt, Lars Engelholm, Yixuan Cao, Vincent Everts
As a crucial step in ECM remodeling, collagen degradation occurs through different processes, including both extracellular and intracellular degradation. The extracellular pathways of collagen degradation require secretion of collagenolytic proteases, whereas intracellular collagen degradation occurs in the lysosomal compartment after uptake, involving either pre-cleaved or intact fibrillar collagen. The endocytic collagen receptor uPARAP/Endo180 plays an important role in internalization of large collagen degradation products, whereas its role in the phagocytosis of fibrillar collagen has been debated...
December 6, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27920981/infective-endocarditis-and-sj%C3%A3-gren-s-syndrome-diagnosed-simultaneously
#15
Fujiko Morita, Yuji Hirai, Kiyozumi Suzuki, Yuki Uehara, Kazunori Mitsuhashi, Atsushi Amano, Toshio Naito
Poor dentition and/or dental infection due to insufficient oral care are presumed to be risk factors for infective endocarditis (IE). We present a case of endocarditis caused by Granulicatella adiacens and Sjögren's syndrome (SS) with oral complications diagnosed simultaneously. A 67-year-old woman was admitted to our hospital with fever, general fatigue, arthralgia, and back pain. She was diagnosed with primary SS according to the criteria of the American-European Consensus Group. Transthoracic echocardiography carried out to examine her persistent fever revealed vegetation formation (14 × 5 mm) on the aortic valve and her blood cultures were positive for G...
2017: IDCases
https://www.readbyqxmd.com/read/27920651/use-of-direct-acting-antivirals-for-the-treatment-of-hepatitis-c-virus-associated-oral-lichen-planus-a-case-report
#16
Kenji Misaka, Takashi Kishimoto, Yuji Kawahigashi, Michio Sata, Yumiko Nagao
Hepatitis C virus (HCV) is frequently associated with various extrahepatic manifestations such as autoimmune features and immune complex deposit diseases. Oral lichen planus (OLP) is one of the representative extrahepatic manifestations of HCV infection. Direct-acting antivirals (DAA) are highly effective and safe for the eradication of HCV. However, there is a lack of information regarding the association between HCV-associated OLP and interferon (IFN)-free DAA therapy. Herein, we present the case of a 60-year-old female who was diagnosed with OLP during routine periodontal treatment by a dentist...
September 2016: Case Reports in Gastroenterology
https://www.readbyqxmd.com/read/27920312/dental-care-and-medicare-beneficiaries-access-gaps-cost-burdens-and-policy-options
#17
Amber Willink, Cathy Schoen, Karen Davis
Despite the wealth of evidence that oral health is related to physical health, Medicare explicitly excludes dental care from coverage, leaving beneficiaries at risk for tooth decay and periodontal disease and exposed to high out-of-pocket spending. To profile these risks, we examined access to dental care across income groups and types of insurance coverage in 2012. High-income beneficiaries were almost three times as likely to have received dental care in the previous twelve months, compared to low-income beneficiaries-74 percent of whom received no dental care...
December 1, 2016: Health Affairs
https://www.readbyqxmd.com/read/27920303/the-dental-medical-divide
#18
Elizabeth A Mertz
The importance of oral health for overall well-being cannot be overstated. Yet the US dental delivery system struggles to address effectively the two most common oral diseases (caries and periodontal disease), which are among the most prevalent of all chronic diseases and are largely preventable. This article describes the evolution of contemporary US dental care policy and practice, highlighting the challenges resulting from the dental system's separation from the rest of health care, and explores the implications of this divide for the future of oral health policy and system reform...
December 1, 2016: Health Affairs
https://www.readbyqxmd.com/read/27918732/relationship-between-889-c-t-polymorphism-in-interleukin-1a-gene-and-risk-of-chronic-periodontitis-evidence-from-a-meta-analysis-with-new-published-findings
#19
F-R-P da Silva, A-C-C Guimarães-Vasconcelos, L-F de-Carvalho-França, D di-Lenardo, L-S Rodrigues, M-L-L Barreto-do-Nascimento, D-F-P Vasconcelos
BACKGROUND: Periodontitis results from an inflammatory response caused by accumulative microorganisms in periodontal sites. Several factors are involved in pathogenesis of periodontitis, for example the -889 C/T polymorphism in interleukin-1A gene. This study aimed to evaluate the relationship between this polymorphism and risk of development of chronic periodontitis by a meta-analysis based in new published findings. MATERIAL AND METHODS: Thereunto a review in literature was performed in the electronic biomedical and education databases (Cochrane Library, Google Scholar, MEDLINE and PubMed) to studies published before August 2, 2015, the abstracts were evaluated and the data extraction performed by two calibrated examiners...
December 6, 2016: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/27917624/relationships-between-high-mobility-group-protein-b1-and-triggering-receptor-expressed-on-myeloid-cells-concentrations-in-gingival-crevicular-fluid-and-chronic-periodontitis
#20
Mojgan Paknejad, Mandana Sattari, Zohreh Roozbahani, Morteza Ershadi, Ali Mehrfard
One of the inflammatory mediators which is secreted by inflammatory cells is high-mobility group protein B1 (HMGB1). Interaction of HMGB1 and toll-like receptors (TLRs) leads to increased production of inflammatory cytokines. On the other hand, it was shown that triggering receptor expressed on myeloid cells (TREM-1) also can be activated by TLRs, and its soluble form (sTREM-1) can be formed by cleaving of membrane-bound form of TREM-1 proteinases. Since there is not enough knowledge about the precise role of HMGB1 and sTREM-1 in periodontal diseases, the aim of this study was to evaluate the concentration of HMGB1 and sTREM-1 in gingival crevicular fluid (GCF) samples of patients with chronic periodontitis...
October 2016: Iranian Journal of Allergy, Asthma, and Immunology
keyword
keyword
2701
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"