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https://www.readbyqxmd.com/read/29334625/light-quality-affects-flavonoid-production-and-related-gene-expression-in-cyclocarya-paliurus
#1
Yang Liu, Shengzuo Fang, Wanxia Yang, Xulan Shang, Xiangxiang Fu
Understanding the responses of plant growth and secondary metabolites to differential light conditions is very important to optimize cultivation conditions of medicinal woody plants. As a highly valued and multiple function tree species, Cyclocarya paliurus is planted and managed for timber production and medical use. In this study, LED-based light including white light (WL), blue light (BL), red light (RL), and green light (GL) were used to affect leaf biomass production, flavonoid accumulation and related gene expression of one-year C...
January 11, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29334460/multiple-stimuli-responses-of-stereo-isomers-of-aie-active-ethynylene-bridged-and-pyridyl-modified-tetraphenylethene
#2
Zhaoyang Wang, Xiao Cheng, Anjun Qin, Haoke Zhang, Jing Zhi Sun, Ben Zhong Tang
Luminescent molecules with aggregation-induced emission (AIE) property, or AIE-gens are typical stimuli-responsive materials and many AIE-gens have shown luminescent responses to mechano-, thermo-, electro-, vapo- and/or solvato-stimulus, but the detailed structure-property relationship has been addressed for only few of them. Here, we report a tetraphenylethene (TPE) derivative with pyridyl modifiers and ethynylene bridges. The (Z)- and (E)-isomers are clearly purified and both of them are AIE-active and demonstrate multiple luminescent responses to external stimuli...
January 15, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29333931/hyperspectral-imaging-of-tissue-perfusion-and-oxygenation-in-wounds-assessing-the-impact-of-a-micro-capillary-dressing
#3
Thomas Wild, Markus Becker, Jochen Winter, Nicole Schuhschenk, Georg Daeschlein, Frank Siemers
OBJECTIVE: Experimental tests of non-invasive multi- or hyperspectral imaging (HSI) systems reveal the high potential of support for medical diagnostic purposes and scientific biomedical analysis. Until now the use of HSI technologies for medical applications was limited by complex and overly sophisticated systems. We present a new and compact HSI-camera that could be used in normal clinical practice. METHOD: We assessed the use of the HSI system on the hands of 10 healthy volunteers, looking at control parameters, and those following venous occlusion, arterial occlusion and reperfusion, including tissue oxygenation, tissue haemoglobin index, perfusion in 4-6mm depth=near infrared spectroscopy (NIR), and tissue water index...
January 2, 2018: Journal of Wound Care
https://www.readbyqxmd.com/read/29333886/anaemia-worsens-early-functional-outcome-after-traumatic-brain-injury-a-preliminary-study
#4
N Scott Litofsky, Douglas C Miller, Zhenzhou Chen, Agnes Simonyi, Diana Klakotskaia, Andrew Giritharan, Qi Feng, Diane McConnell, Jiankun Cui, Zezong Gu
OBJECTIVE: To determine early effects on outcome from traumatic brain injury (TBI) induced by controlled cortical impact (CCI) associated with anaemia in mice. HYPOTHESIS: Outcome from TBI with concomitant anaemia would be worse than TBI without anaemia. METHODS: CCI was induced with electromagnetic impaction in four groups of C57BL/6J mice: sham, sham+anaemia; TBI; and TBI+anaemia. Anaemia was created by withdrawal of 30% of calculated intravascular blood volume and saline replacement of equal volume...
January 15, 2018: Brain Injury: [BI]
https://www.readbyqxmd.com/read/29333097/preliminary-study-of-mr-and-fluorescence-dual-mode-imaging-combined-macrophage-targeted-and-superparamagnetic-polymeric-micelles
#5
Wen-Juan Li, Yong Wang, Yulin Liu, Teng Wu, Wen-Li Cai, Xin-Tao Shuai, Guo-Bin Hong
Purpose: To establish small-sized superparamagnetic polymeric micelles for magnetic resonance and fluorescent dual-modal imaging, we investigated the feasibility of MR imaging (MRI) and macrophage-targeted in vitro. Methods: A new class of superparamagnetic iron oxide nanoparticles (SPIONs) and Nile red-co-loaded mPEG-Lys3-CA4-NR/SPION polymeric micelles was synthesized to label Raw264.7 cells. The physical characteristics of the polymeric micelles were assessed, the T2 relaxation rate was calculated, and the effect of labeling on the cell viability and cytotoxicity was also determined in vitro...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29332836/fluorescent-and-colorimetric-dual-mode-aptasensor-for-thrombin-detection-based-on-target-induced-conjunction-of-split-aptamer-fragments
#6
Wenna Duan, Xiuzhong Wang, Haixia Wang, Feng Li
Since the lack of detection diversity of the single-signal readout strategy, it is urgent to develop fast and multisignal assay strategies. A highly selective and sensitive assay method with colorimetric and fluorometric dual signals readouts is presented in this paper. It is based on the principle that the target induced conjunction of split aptamer fragments assembled on the surface of Au nanoparticles (AuNPs). In the presence of targets, the color of solution changed from wine red to blue and can be measured both visual inspection and spectrophotometry because of the aggregation of AuNPs...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332299/similarity-search-combined-with-docking-and-molecular-dynamics-for-novel-hache-inhibitor-scaffolds
#7
Nadia Melo Borges, Geraldo Rodrigues Sartori, Jean F R Ribeiro, Josmar R Rocha, João B L Martins, Carlos A Montanari, Ricardo Gargano
The main purpose of this study was to address the performance of virtual screening methods based on ligands and the protein structure of acetylcholinesterase (AChE) in order to retrieve novel human AChE (hAChE) inhibitors. In addition, a protocol was developed to identify novel hit compounds and propose new promising AChE inhibitors from the ZINC database with 10 million commercially available compounds. In this sense, 3D similarity searches using rapid overlay of chemical structures and similarity analysis through comparison of electrostatic overlay of docked hits were used to retrieve AChE inhibitors from collected databases...
January 13, 2018: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29332284/measurement-of-hypersensitive-cell-death-triggered-by-avirulent-bacterial-pathogens-in-arabidopsis
#8
Zahra Imanifard, Elodie Vandelle, Diana Bellin
The hypersensitive response is one of the most powerful and complex defense reactions to survive to pathogen attacks during an incompatible plant-pathogen interaction. Local programmed cell death accompanies the hypersensitive response at the site of infection to prevent pathogen growth and spread. A precise quantitative assessment of this form of programmed cell death is essential to unravel the genetic and molecular mechanisms underlying the process. Here, we first describe the optimization of a Trypan Blue staining protocol for quantitatively measuring the HR-cell death in Arabidopsis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332258/photodynamic-antimicrobial-chemotherapy-pact-using-toluidine-blue-inhibits-both-growth-and-biofilm-formation-by-candida-krusei
#9
Bruna Graziele Marques da Silva, Moisés Lopes Carvalho, Isabela Bueno Rosseti, Stella Zamuner, Maricilia Silva Costa
Among non-albicans Candida species, the opportunistic pathogen Candida krusei emerges because of the high mortality related to infections produced by this yeast. The Candida krusei is an opportunistic pathogen presenting an intrinsic resistance to fluconazol. In spite of the reduced number of infections produced by C. krusei, its occurrence is increasing in some groups of patients submitted to the use of fluconazol for prophylaxis. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells...
January 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29332123/braf-nras-and-gnaq-mutations-in-conjunctival-melanocytic-nevi
#10
Jasmine H Francis, Hans E Grossniklaus, Larissa A Habib, Brian Marr, David H Abramson, Klaus J Busam
Purpose: To evaluate BRAF, NRAS, and GNAQ mutations in surgical specimens of common and blue conjunctival melanocytic nevi. Methods: Surgical specimens from 25 conjunctival melanocytic nevi (23 common and 2 blue) of 25 patients were evaluated. All common nevi were analyzed immunohistochemically for the expression of BRAF V600E or NRAS Q61R. One lesion with negative immunoreactivity and for all blue nevi, a hybridization capture-based next-generation sequencing method was employed for mutation analysis...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29331948/lvis-blue-as-a-low-porosity-stent-and-coil-adjuvant
#11
Matthew J Koch, Christopher J Stapleton, Scott B Raymond, Susan Williams, Thabele M Leslie-Mazwi, James David Rabinov, Aman B Patel
INTRODUCTION: The LVIS Blue is an FDA-approved stent with 28% metallic coverage that is indicated for use in conjunction with coil embolization for the treatment of intracranial aneurysms. Given a porosity similar to approved flow diverters and higher than currently available intracranial stents, we sought to evaluate the effectiveness of this device for the treatment of intracranial aneurysms. METHODS: We performed an observational single-center study to evaluate initial occlusion and occlusion at 6-month follow-up for patients treated with the LVIS Blue in conjunction with coil embolization at our institution using the modified Raymond-Roy classification (mRRC), where mRRC 1 indicates complete embolization, mRRC 2 persistent opacification of the aneurysm neck, mRRC 3a filling of the aneurysm dome within coil interstices, and mRRC 3b filling of the aneurysm dome...
January 13, 2018: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/29331834/land-water-food-nexus-and-indications-of-crop-adjustment-for-water-shortage-solution
#12
Dandan Ren, Yonghui Yang, Yanmin Yang, Keith Richards, Xinyao Zhou
While agriculture places the greatest demand on water resources, increasing agricultural production is worsening a global water shortage. Reducing the cultivation of water-consuming crops may be the most effective way to reduce agricultural water use. However, when also taking food demand into consideration, sustaining the balance between regional water and food securities is a growing challenge. This paper addresses this task for regions where water is unsustainable for food production (Beijing-Tianjin-Hebei Region for example) by: (i) assessing the different effects of wheat and maize on water use; (ii) analyzing virtual water and virtual land flows associated with food imports and exports between Beijing-Tianjin-Hebei and elsewhere in China; (iii) identifying sub-regions where grain is produced using scarce water resources but exported to other regions; and (iv) analyzing the potentiality for mitigating water shortage via Land-Water-Food Nexus...
January 11, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29331778/synthesis-of-a-novel-narrow-band-gap-iron-ii-iii-oxide-titania-silver-silicate-nanocomposite-as-a-highly-efficient-and-stable-visible-light-driven-photocatalyst
#13
Huiping Chen, Nan Chen, Chuanping Feng, Yu Gao
Ag6Si2O7, a visible light-driven photocatalyst, has attracted considerable attention owing to its enormous environmental remediation potential. In this work, a magnetic iron(II,III) oxide/titania/silver silicate (Fe3O4/TiO2/Ag6Si2O7) nanocomposite was synthesized by anchoring TiO2 and Ag6Si2O7 on the surface of Fe3O4 nanoparticles. The morphology, crystal structure, as well as the spectroscopic, magnetic, and photocurrent properties of the as-prepared Fe3O4/TiO2/Ag6Si2O7 nanocomposite were studied. Methylene blue (MB) was used for evaluating the photocatalytic performance under simulated visible light...
January 6, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331761/prevention-of-articular-cartilage-degeneration-in-a-rat-model-of-monosodium-iodoacetate-induced-osteoarthritis-by-oral-treatment-with-withaferin-a
#14
Dharmendra Choudhary, Sulekha Adhikary, Naseer Ahmad, Priyanka Kothari, Ashwni Verma, Prabodh Kumar Trivedi, Prabhat Ranjan Mishra, Ritu Trivedi
Withaferin A (WFA), a highly oxygenated withanolide is used for anti-osteoporotic, fracture healing, obesity control as medicine and dietary supplement in Ayurveda and Unani medicine but its potential remains to be investigate for the osteoarthritis studies. In the present study, chondro-protective effects of WFA, under in vitro and in vivo conditions were evaluated. In-vitro pharmacological activity of WFA was tested on rat articular chondrocytes through MTT, DPPH, different staining, FACS and translation studies...
January 10, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29331725/bivo4-n-rgo-nano-composites-as-highly-efficient-visible-active-photocatalyst-for-the-degradation-of-dyes-and-antibiotics-in-eco-system
#15
Brindha Appavu, Sivakumar Thiripuranthagan, Sudhakar Ranganathan, Elangovan Erusappan, Kathiravan Kannan
Herein, we report the synthesis of novel nitrogen doped reduced graphene oxide/ BiVO4 photo catalyst by single step hydrothermal method. The physicochemical properties of the catalysts were characterized using XRD, N2 adsorption-desorption, Raman, XPS, SEM TEM, DRS-UV and EIS techniques. The synthesized catalysts were tested for their catalytic activity in the photo degradation of some harmful textile dyes (methylene blue & congo red) and antibiotics (metronidazole and chloramphenicol) under visible light irradiation...
January 10, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29331661/effect-of-laf3-ag-fluorescent-nanoparticles-on-photodynamic-efficiency-and-cytotoxicity-of-protoporphyrin-ix-photosensitizer
#16
F Tavakkoli, M Zahedifar, E Sadeghi
LaF3: Ag nanoparticles (NPs) were synthesized by the co-precipitation method. The produced NPs were characterized by X-ray diffraction (XRD) pattern, scanning electron microscope (SEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). The emission spectrum of LaF3:Ag NPs is mostly overlapped with the absorption band of protoporphyrin IX (PpIX) and their conjugation was confirmed by studying fluorescence resonance energy transfer (FRET) from LaF3:Ag donor to protoporphyrin IX acceptor...
January 10, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29331487/curecuma-cationic-curcuminoids-with-improved-properties-and-enhanced-antimicrobial-photodynamic-activity
#17
Andreas Spaeth, Andre Graeler, Tim Maisch, Kristjan Plaetzer
The naturally occurring photosensitizer curcumin has excellent biocompatibility, but its antimicrobial photodynamic efficacy is limited by (i) weak adherence to Gram(-) cell walls, (ii) low (photo-)stability and (iii) limited solubility in water. In this study novel curcuminoids bearing cationic substituents were prepared by different synthetic routes. The derivatives exhibit excellent water solubility, improved photostability and low aggregation. All novel curcuminoids showed antibacterial photodynamic effects (>3 log10 reduction of CFU) against Escherichia coli and Staphylococcus aureus upon blue light illumination...
October 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29331306/primary-mucinous-eccrine-carcinoma-of-the-buccal-space-a-case-report-and-review-of-the-literature
#18
Kirkland N Lozada, Muhammed S Qazi, Azita S Khorsandi, Raymond L Chai
IMPORTANCE: Mucinous eccrine carcinoma is a rare entity that most commonly affects the head and neck. Due to its low frequency of occurrence, review of its etiology, histopathology, and treatment strategies is beneficial to all clinicians who may encounter similar appearing masses. OBSERVATION: An 84-year-old male presented with a blue mass on the left cheek. This mass started as a small bump and grew significantly over one year. His primary care physician monitored its growth and ultimately referred to an otolaryngologist...
January 5, 2018: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/29331130/femtosecond-coherent-nuclear-dynamics-of-excited-tetraphenylethylene-ultrafast-transient-absorption-and-ultrafast-raman-loss-spectroscopic-studies
#19
Surajit Kayal, Khokan Roy, Siva Umapathy
Ultrafast torsional dynamics plays an important role in the photoinduced excited state dynamics. Tetraphenylethylene (TPE), a model system for the molecular motor, executes interesting torsional dynamics upon photoexcitation. The photoreaction of TPE involves ultrafast internal conversion via a nearly planar intermediate state (relaxed state) that further leads to a twisted zwitterionic state. Here, we report the photoinduced structural dynamics of excited TPE during the course of photoisomerization in the condensed phase by ultrafast Raman loss (URLS) and femtosecond transient absorption (TA) spectroscopy...
January 14, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29330702/ptac10-an-s1-domain-containing-component-of-the-transcriptionally-active-chromosome-complex-is-essential-for-plastid-gene-expression-in-arabidopsis-thaliana-and-is-phosphorylated-by-chloroplast-targeted-casein-kinase-ii
#20
Qing-Bo Yu, Tuan-Tuan Zhao, Lin-Shan Ye, Ling Cheng, Ying-Qian Wu, Chao Huang, Zhong-Nan Yang
In higher plant chloroplasts, the plastid-encoded RNA polymerase (PEP) consists of four catalytic subunits and numerous nuclear-encoded accessory proteins, including pTAC10, an S1-domain-containing protein. In this study, pTAC10 knockout lines were characterized. Two ptac10 mutants had an albino phenotype and severely impaired chloroplast development. The pTAC10 genomic sequence fused to a four-tandem MYC tag driven by its own promoter functionally complemented the ptac10-1 mutant phenotype. pTAC10 was present in both the chloroplast stroma and thylakoids...
January 12, 2018: Photosynthesis Research
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