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https://www.readbyqxmd.com/read/30007208/single-cell-dielectrophoresis-study-of-apoptosis-progression-induced-by-controlled-starvation
#1
Elham Salimi, Katrin Braasch, Azita Fazelkhah, Samaneh Afshar, Bahareh Saboktakin Rizi, Kaveh Mohammad, Michael Butler, Greg E Bridges, Douglas J Thomson
Nutrient depletion in fed-batch cultures and at the end of batch cultures is among the main causes of stress on cells and a trigger of apoptosis. In this study, we investigated changes in the cytoplasm conductivity of Chinese hamster ovary (CHO) cells under controlled starvation. Employing a single-cell dielectrophoresis (DEP) cytometer, we measured the DEP response of CHO cells incubated in a medium without glucose and glutamine over a 48-h period. Using the measured data in conjunction with numerical simulations, we determined the cytoplasm conductivity of viable and apoptotic cell subpopulations...
July 5, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/30007203/preeclampsia-serum-induces-human-glomerular-vascular-endothelial-cell-hyperpermeability-via-the-hmgb1-caveolin-1-pathway
#2
Qinyang Xu, Fei Du, Ye Zhang, Yincheng Teng, Minfang Tao, Alex F Chen, Rongzhen Jiang
To explore new ideas about the pathogeny of preeclampsia (PE) proteinuria, this study focused on whether severe PE serum (PES) could induce high-molecular-weight protein (HMWP) hyperpermeability in glomerular endothelial cells (GEC) via the HMGB1-Caveolin-1 (CAV-1) pathway. Normal pregnancy serum (NPS) and severe PES were used to treat primary human GEC monolayer for 24 h. The CAV-1 inhibitor methyl-beta-cyclodextrin (MBCD), the HMGB1 inhibitor glycyrrhizicacid (GA), recombinant HMGB1 (rHMGB1) were also used to treat GEC monolayer that were stimulated by NPS or severe PES...
July 6, 2018: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/30006822/structural-investigation-of-the-hormone-melatonin-and-its-alkali-and-alkaline-earth-metal-complexes-in-the-gas-phase
#3
Satrajit Chakrabarty, Matthew J DiTucci, Giel Berden, Jos Oomens, Evan R Williams
Gas phase infrared dissociation spectra of the radical cation, deprotonated and protonated forms of the hormone melatonin, and its complexes with alkali (Li+ , Na+ , and K+ ) and alkaline earth metal ions (Mg2+ , Ca2+ , and Sr2+ ) are measured in the spectral range 800-1800 cm-1 . Minimum energy geometries calculated at the B3LYP/LACVP++** level are used to assign structural motifs to absorption bands in the experimental spectra. The melatonin anion is deprotonated at the indole-N. The indole-C linking the amide chain is the most favored protonation site...
July 13, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/30006806/molecular-verification-of-transplacental-transmission-of-theileria-lestoquardi-in-goat
#4
Bijan Esmaeilnejad, Mousa Tavassoli, Awat Samiei, Nasser Hajipour
Ovine and caprine malignant theileriosis (OCMT), a critical condition in small ruminant production, causes lethal infections. In September 2016, a total number of 400 goats of Marghoz breed (the largest population of goat breed in Iran), in northwest of Iran, were examined for approximately 25 pre-partum abortions and 7 goats' mortality for a period of 3 days. A dead goat and her aborted fetus were brought into the Urmia Veterinary Hospital for further diagnostic investigations. The microbiological assessments including direct microscopical examination of the vaginal discharges and placentome with respect to the differential staining (Gram's staining method), conventional pure culturing, fetal abomasal contents, and the liver were negative...
July 13, 2018: Parasitology Research
https://www.readbyqxmd.com/read/30006675/a-paper-based-optical-probe-for-chromium-by-using-gold-nanoparticles-modified-with-2-2-thiodiacetic-acid-and-smartphone-camera-readout
#5
Shadab Faham, Gholamreza Khayatian, Hamed Golmohammadi, Raouf Ghavami
A paper based analytical device is presented for the determination of Cr(III) and Cr(VI) using gold nanoparticles (AuNPs) modified with 2,2'-thiodiacetic acid. The modified AuNPs were characterized using UV-Vis spectrophotometry, Fourier transform infrared, dynamic light scattering, zeta potential, energy dispersive spectroscopy and transmission electron microscopy. Cr(III) ions induce the aggregation of the modified AuNPs, and the color of the nanoprobe changes from red to blue. This can be detected visually, or by colorimetry, or with a camera...
July 13, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/30006659/high-glycine-concentration-increases-collagen-synthesis-by-articular-chondrocytes-in-vitro-acute-glycine-deficiency-could-be-an-important-cause-of-osteoarthritis
#6
Patricia de Paz-Lugo, José Antonio Lupiáñez, Enrique Meléndez-Hevia
Collagen synthesis is severely diminished in osteoarthritis; thus, enhancing it may help the regeneration of cartilage. This requires large amounts of glycine, proline and lysine. Previous works of our group have shown that glycine is an essential amino acid, which must be present in the diet in large amounts to satisfy the demands for collagen synthesis. Other authors have shown that proline is conditionally essential. In this work we studied the effect of these amino acids on type II collagen synthesis. Bovine articular chondrocytes were cultured under a wide range of different concentrations of glycine, proline and lysine...
July 13, 2018: Amino Acids
https://www.readbyqxmd.com/read/30006317/cartilage-tissue-formation-through-assembly-of-microgels-containing-mesenchymal-stem-cells
#7
Fanyi Li, Vinh X Truong, Philipp Fisch, Clara Levinson, Veronica Glattauer, Marcy Zenobi-Wong, Helmut Thissen, John S Forsythe, Jessica E Frith
Current clinical approaches to treat articular cartilage degeneration provide only a limited ability to regenerate tissue with long-term durability and functionality. In this application, injectable bulk hydrogels and microgels containing stem cells can provide a suitable environment for tissue regeneration. However insufficient cell-cell interactions, low differentiation efficiency and poor tissue adhesion hinder the formation of high-quality hyaline type cartilage. Here, we have designed a higher order tissue-like structure using injectable cell-laden microgels as the building blocks to achieve human bone marrow-derived mesenchymal stem cell (hBMSC) long-term maintenance and chondrogenesis...
July 10, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/30006137/sigma-1-receptor-activation-alleviates-blood-brain-barrier-dysfunction-in-vascular-dementia-mice
#8
Dan-Yang Liu, Tian-Yan Chi, Xue-Fei Ji, Peng Liu, Xiao-Xiao Qi, Lin Zhu, Zi-Qi Wang, Lin-Li, Ling Chen, Li-Bo Zou
Sigma-1 receptor (Sig-1R) activation has been shown to decrease infarct volume and enhance neuronal survival after brain ischemia-reperfusion (IR) in rodent models. The present study aims to investigate first the effect of Sig-1R activation on blood-brain barrier (BBB) disruption during experimental stroke. Male C57BL/6 mice were subjected to bilateral common carotid artery occlusion (BCCAO) for 15 min, and the worst BBB leakage was observed on the 7th day after brain IR. To confirm the BBB protective role of Sig-1R, mice were divided into five groups (sham group, BCCAO group, PRE084 group, BD1047 group, PRE084 and BD1047 group; 29-35 mice for each group), and treated with agonist PRE084 (1 mg/kg) and/or antagonist BD1047 (1 mg/kg) for 7 days intraperitoneally once a day after BCCAO...
July 10, 2018: Experimental Neurology
https://www.readbyqxmd.com/read/30006125/breast-mass-characterization-using-shear-wave-elastography-and-ultrasound
#9
S Hari, S B Paul, R Vidyasagar, E Dhamija, A D Adarsh, S Thulkar, S Mathur, V Sreenivas, S Sharma, A Srivastava, V Seenu, R Prashad
PURPOSE: To evaluate the role of SWE in characterizing breast masses and ascertain whether additional use of SWE to ultrasound for evaluating BI-RADS 3 and 4a masses could help reduce long-term follow-up and unnecessary biopsies of these suspicious breast masses. MATERIALS AND METHODS: This prospective, cross-sectional study was performed between June 2013 and November 2014. All enrolled patients underwent clinical breast examination, ultrasound, SWE and ultrasound-guided core biopsy of the breast mass...
July 10, 2018: Diagnostic and Interventional Imaging
https://www.readbyqxmd.com/read/30006011/development-of-ph-indicator-from-pla-peo-ultrafine-fibers-containing-pigment-of-microalgae-origin
#10
Juliana Botelho Moreira, Ana Luiza Machado Terra, Jorge Alberto Vieira Costa, Michele Greque de Morais
Phycocyanin is a pigment of intense blue color, constituting the biomass of microalga such as Spirulina. This pigment is sensitive to pH and this instability results in color change. Thus, phycocyanin fading may become interesting for application in intelligent packaging. The objective of the study was to develop PLA/PEO ultrafine fibers containing phycocyanin to be used as pH indicators membranes for food packaging. The ultrafine fibers were formed by electrospinning process. The average diameter of 1318 nm was obtained for PLA/PEO ultrafine fibers with 2% (w·v-1 ) of phycocyanin and 921 nm for those developed with 3% (w·v-1 ) of the dye...
July 10, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30005306/blood-brain-barrier-breakdown-memory-impairment-and-neurotoxicity-caused-in-mice-submitted-to-orally-treatment-with-thymol
#11
Matheus D Baldissera, Carine F Souza, Antônio Francisco Igor M De Matos, Pedro Henrique Doleski, Bernardo Baldisserotto, Aleksandro S Da Silva, Silvia G Monteiro
Several evidences have related the biochemical and pharmacological properties of thymol, but the possible neurotoxic effects of this compound remain unknown and not evaluated. Thus, the purpose of this study was to evaluate whether intake of thymol in different doses (10, 20 and 40 mg/kg) induce neurotoxicity and behavioral alterations using mice as experimental model, as well as the involvement of blood-brain barrier (BBB) and brain neurotransmitters in these alterations. Thymol (20 and 40 mg/kg) significantly decrease latency time to inhibitory avoidance task when compared to control group, indicating a memory loss after 30 days of oral treatment...
July 8, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/30005249/nano-porous-sapo-34-enhanced-thin-film-nanocomposite-polymeric-membrane-simultaneously-high-water-permeation-and-complete-removal-of-cationic-anionic-dyes-from-water
#12
Negin Ghaemi, Parisa Safari
Thin-film nanocomposite (TFN) membranes were prepared by embedding nano-porous SAPO-34 nanoparticles in a polypyrrole thin-film layer polymerized on PES substrate. SEM, EDX, AFM, hydrophilicity, zeta potential and MWCO measurements were applied to verify characteristics of membranes. TFN membranes presented considerably higher pure water permeability (by more than 300%) due to high hydrophilicity and also nano-channels created in thin-film. Performance of TFN membranes were also evaluated by removal of different anionic and cationic dyes (methyl violet 6B, reactive blue 4 and acid blue 193) from water...
July 5, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/30005177/characteristics-of-intracellular-algogenic-organic-matter-and-its-reactivity-with-hydroxyl-radicals
#13
Doorae Lee, Minhwan Kwon, Yongtae Ahn, Youmi Jung, Seong-Nam Nam, Il-Hwan Choi, Joon-Wun Kang
The aim of this study was to investigate the reactivity of intracellular algogenic organic matter (IOM) with hydroxyl radicals (·OH), a key reaction species in advanced oxidation processes. IOM was extracted from two green algae, Chlamydomonas reinhardtii and Scenedesmus sp., and two blue-green algae, Anabaena sp. and Microcystis aeruginosa using a freeze-thaw method. The second-order rate constants of the extracted IOM with ·OH were determined as 7.95 × 108 MC -1 s-1 (Chlamydomonas reinhardtii), 6.71 × 108 MC -1 s-1 (Scenedesmus sp...
June 30, 2018: Water Research
https://www.readbyqxmd.com/read/30004973/blue-light-illuminates-a-novel-sepsis-treatment
#14
Sonal Pannu, Elliott D Crouser
No abstract text is available yet for this article.
August 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/30004877/the-influence-of-label-design-on-search-performance-and-noticeability-in-wide-field-of-view-augmented-reality-displays
#15
Ernst Kruijff, Jason Orlosky, Naohiro Kishishita, Christina Trepkowski, Kiyoshi Kiyokawa
In Augmented Reality (AR), search performance for outdoor tasks is an important metric for evaluating the success of a large number of AR applications. Users must be able to find content quickly, labels and indicators must not be invasive but still clearly noticeable, and the user interface should maximize search performance in a variety of conditions. To address these issues, we have set up a series of experiments to test the influence of virtual characteristics such as color, size, and leader lines on the performance of search tasks and noticeability in both real and simulated environments...
July 10, 2018: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/30004681/fluorescent-sulfonate-based-inorganic-organic-hybrid-nanoparticles-for-staining-and-imaging
#16
Marieke Poß, Eva Zittel, Anna Meschkov, Ute Schepers, Claus Feldmann
Sulfonate-based inorganic-organic hybrid nanoparticles (IOH-NPs) with the general saline composition [Gd(OH)]2+n/2[Rdye(SO3)n]n- showing optical absorption and emission in the blue to red spectral regime are presented for the first time. All IOH-NPs are prepared via straightforward aqueous synthesis and instantaneously result in colloidally highly stable suspensions with mean particle diameters of 40-50 nm and high zeta potentials (-20 to -40 mV at pH 7.0). Specifically, the IOH-NPs comprise [Gd(OH)]2+2[CSB]4-, [Gd(OH)]2+2[DB71]4-, [Gd(OH)]2+[NFR]2-, [Gd(OH)]2+[AR97]2-, and [Gd(OH)]2+2[EB]4- showing blue, orange, red and infrared absorption and emission ([CSB]: Chicago Sky Blue; [DB71]: Direct Blue 71; [NFR]: Nuclear Fast Red; [AR97]: Acid Red 97; [EB]: Evans Blue)...
July 13, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/30004663/boron-doped-graphene-nanoribbons-electronic-structure-and-raman-fingerprint
#17
Boris V Senkovskiy, Dmitry Yu Usachov, Alexander V Fedorov, Tomas Marangoni, Danny Haberer, Cesare Tresca, Gianni Profeta, Vasile Caciuc, Shigeru Tsukamoto, Nicolae Atodiresei, Niels Ehlen, Chaoyu Chen, José Avila, Maria C Asensio, Andrei Yu Varykhalov, Alexei Nefedov, Christof Wöll, Timur K Kim, Moritz Hoesch, Felix R Fischer, Alexander Grüneis
We investigate the electronic and vibrational properties of bottom-up synthesized aligned armchair graphene nanoribbons of N=7 carbon atoms width periodically doped by substitutional boron atoms (B-7AGNRs). Using angle-resolved photoemission spectroscopy (ARPES), we find that the dopant-derived valence and conduction band states are notably hybridized with electronic states of Au substrate and spread in energy. The interaction with the substrate leaves the bands with pure carbon character rather unperturbed...
July 13, 2018: ACS Nano
https://www.readbyqxmd.com/read/30004516/monolithic-integration-of-iii-nitride-voltage-controlled-light-emitters-with-dual-wavelength-photodiodes-by-selective-area-epitaxy
#18
Chao Liu, Yuefei Cai, Huaxing Jiang, Kei May Lau
We report for the first time on-chip integration of III-nitride voltage-controlled light emitters with visible and ultraviolet (UV) photodiodes (PDs). InGaN/GaN and AlGaN/GaN heterostructures were grown in specific regions by selective-area epitaxy, allowing monolithic integration of versatile devices including visible light emitting diodes (LEDs), visible-light PDs, AlGaN/GaN high electron mobility transistors (HEMTs), and UV-light Schottky barrier (SB) PDs. A serial connection between the LED and HEMT through the epitaxial layers enables a three-terminal voltage-controlled light emitter (HEMT-LED), efficiently converting voltage-controlled signals into visible-light signals that can be coupled into an adjacent visible-light PD generating electrical signals...
July 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/30004438/using-natural-biomacromolecules-for-adsorptive-and-enzymatic-removal-of-aniline-blue-from-water
#19
Xiaojuan You, Enzhong Li, Jiayang Liu, Songhua Li
The present study investigated the adsorptive and enzymatic removal of aniline blue dye (AB) from aqueous solution using waxy riceprocessing waste (RW), peanut shell (PS), microbial waste of Aspergillus niger (MW) as low cost adsorbents, and laccase (Lac) as a biocatalyst. Commercial activated carbon (AC) was also employed to compare the adsorption performance with the three adsorbents. Dye removal was examined under various parameters in batch experiments. It was found that dye removal by RW and Lac was 89⁻94% noticeably better than that by MW and PS (20⁻70%)...
July 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30004218/intrinsic-cancer-cell-selective-toxicity-of-organic-photothermal-nano-agent-a-simple-formulation-for-combined-photothermal-chemotherapy-of-cancer
#20
Zhuoxuan Lu, Feng-Ying Huang, Rong Cao, Guang-Hong Tan, Guohui Yi, Nongyue He, Lingfeng Xu, Liming Zhang
Nano-agent-mediated photothermal therapy (PTT) combined with chemotherapy has been proposed as an effective strategy against cancer. However, chemotherapeutic agents often cause serious side effects. Herein, a novel PTT nanoagent (Cy5.5-MSA-G250) with unanticipated intrinsic tumor-selective cytotoxicity is developed. The Cy5.5-MSA-G250 nanoparticles (NPs) are created by mixing mouse serum albumin (MSA) and coomassie brilliant blue (G250), and then conjugated with cyanine 5.5 (Cy5.5). As expected, Cy5.5-MSA-G250 NPs can efficiently kill cancer cells in vitro and in vivo by PTT...
July 13, 2018: ACS Applied Materials & Interfaces
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