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https://www.readbyqxmd.com/read/29236110/simultaneous-monitoring-of-different-vitrification-solution-components-permeating-into-tissues
#1
Andrés Vásquez-Rivera, Kim K Sommer, Harriëtte Oldenhof, Adam Z Higgins, Kelvin G M Brockbank, Andres Hilfiker, Willem F Wolkers
Cryopreservation can be used for long-term preservation of tissues and organs. It relies on using complex mixtures of cryoprotective agents (CPAs) to reduce the damaging effects of freezing, but care should be taken to avoid toxic effects of CPAs themselves. In order to rationally design cryopreservation strategies for tissues, it is important to precisely determine permeation kinetics of the protectants that are used to ensure maximum permeation, while minimizing the exposure time and toxicity effects. This is particularly challenging with protectant solutions consisting of multiple components each with different physical properties and diffusing at a different rate...
December 13, 2017: Analyst
https://www.readbyqxmd.com/read/29234679/detection-of-cryptosporidium-and-cyclospora-oocysts-from-environmental-water-for-drinking-and-recreational-activities-in-sarawak-malaysia
#2
Lesley Maurice Bilung, Ahmad Syatir Tahar, Nur Emyliana Yunos, Kasing Apun, Yvonne Ai-Lian Lim, Elexson Nillian, Hashimatul Fatma Hashim
Cryptosporidiosis and cyclosporiasis are caused by waterborne coccidian protozoan parasites of the genera Cryptosporidium and Cyclospora, respectively. This study was conducted to detect Cryptosporidium and Cyclospora oocysts from environmental water abstracted by drinking water treatment plants and recreational activities in Sarawak, Malaysia. Water samples (12 each) were collected from Sungai Sarawak Kanan in Bau and Sungai Sarawak Kiri in Batu Kitang, respectively. In addition, 6 water samples each were collected from Ranchan Recreational Park and UNIMAS Lake at Universiti Malaysia Sarawak, Kota Samarahan, respectively...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29234335/metabolite-profiling-of-wheat-seedlings-induced-by-chitosan-revelation-of-the-enhanced-carbon-and-nitrogen-metabolism
#3
Xiaoqian Zhang, Kecheng Li, Ronge Xing, Song Liu, Pengcheng Li
Chitosan plays an important role in regulating growth and eliciting defense in many plant species. However, the exact metabolic response of plants to chitosan is still not clear. The present study performed an integrative analysis of metabolite profiles in chitosan-treated wheat seedlings and further investigated the response of enzyme activities and transcript expression related to the primary carbon (C) and nitrogen (N) metabolism. Metabolite profiling revealed that chitosan could induce significant difference of organic acids, sugars and amino acids in leaves of wheat seedlings...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29233757/the-in-vitro-effect-of-antimicrobial-photodynamic-therapy-on-dental-microcosm-biofilms-from-partially-erupted-permanent-molars-a-pilot-study
#4
Fabiana Sodré de Oliveira, Thiago Cruvinel, Daniela Alejandra Cusicanqui Méndez, Evandro José Dionísio, Daniel Rios, Maria Aparecida Andrade Moreira Machado
BACKGROUND: The aPDT could enhance the prevention of dental caries lesions in pits and fissures of partially erupted molars, by killing microorganisms from complex dental biofilms. This pilot study aimed to evaluate the effect of the Antimicrobial Photodynamic Therapy (aPDT) on the viability of specific microorganism groups of dental microcosm biofilms from occlusal surfaces of first permanent molars in eruption. METHODS: Dental microcosm biofilms grown on bovine enamel blocks, from dental plaque collected on occlusal surfaces of a partially erupted lower right first permanent molar, with McBain medium plus 1% sucrose in anaerobic condition at 37°C for 72h...
December 9, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29233512/response-to-oral-sucrosomial-iron-supplementation-in-patients-undergoing-bariatric-surgery-the-bari-fer-study
#5
Andreea Ciudin, Olga Simó-Servat, Jose Maria Balibrea, Ramon Vilallonga, Cristina Hernandez, Rafael Simó, Jordi Mesa
BACKGROUND: Chronic intravenous iron administration is often required after bariatric surgery. Oral sucrosomial iron has a particular form of absorption and may represent an alternative treatment. OBJECTIVE: To assess the effect of switching to oral sucrosomial iron in patients receiving intravenous iron supplementation after bariatric surgery. PATIENTS AND METHODS: A case-control study was conducted on 40 women of childbearing age, of whom 20 were switched to oral sucrosomial iron, while 20 patients continued on intravenous iron sucrose every three months...
December 7, 2017: Endocrinología, Diabetes y Nutrición
https://www.readbyqxmd.com/read/29232921/the-importance-of-the-circadian-clock-in-regulating-plant-metabolism
#6
REVIEW
Jin A Kim, Hyun-Soon Kim, Seo-Hwa Choi, Ji-Young Jang, Mi-Jeong Jeong, Soo In Lee
Carbohydrates are the primary energy source for plant development. Plants synthesize sucrose in source organs and transport them to sink organs during plant growth. This metabolism is sensitive to environmental changes in light quantity, quality, and photoperiod. In the daytime, the synthesis of sucrose and starch accumulates, and starch is degraded at nighttime. The circadian clock genes provide plants with information on the daily environmental changes and directly control many developmental processes, which are related to the path of primary metabolites throughout the life cycle...
December 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29232842/a-new-laccase-based-biosensor-for-tartrazine
#7
Siti Zulaikha Mazlan, Yook Heng Lee, Sharina Abu Hanifah
Laccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1...
December 9, 2017: Sensors
https://www.readbyqxmd.com/read/29232607/simultaneous-uplc-ms-ms-analysis-of-two-stable-isotope-labeled-versions-of-sucrose-in-mouse-plasma-and-brain-samples-as-markers-of-blood-brain-barrier-permeability-and-brain-vascular-space
#8
Ekram Ahmed Chowdhury, Faleh Alqahtani, Raktima Bhattacharya, Reza Mehvar, Ulrich Bickel
Blood Brain Barrier (BBB) permeability is frequently compromised in the course of diseases affecting the central nervous system (CNS). Sucrose is a low molecular weight, hydrophilic marker with slow permeability at the naive BBB and therefore one of the widely used indicators of barrier integrity. Our laboratory recently developed a highly sensitive UPLC-MS/MS method for stable isotope labeled [13C12]sucrose in biological matrices. Correction of total brain concentration for contribution of intravascular space is required in such experiments in order to accurately measure BBB permeability, and it is often accomplished by vascular perfusion with buffer solutions prior to brain sampling...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29232497/evaluation-of-olfaction-and-taste-function-in-type-2-diabetic-patients-with-and-without-peripheral-neuropathy
#9
Semih Yazla, Süay Özmen, Sinem Kıyıcı, Demet Yıldız, Mehmet Haksever, Sündüz Gencay
AIMS: Olfaction and gustation in patients with diabetes mellitus (DM) have great significance on quality of life and their impairment may result in possible hazards. A limited number of studies have been performed to determine the alteration of both gustatory and olfactory function in type 2 diabetic patients with diabetic peripheral neuropathy (DPN). The aim of this study was to determine whether type 2 diabetic patients, with and without DPN, exhibit major olfactory and gustatory dysfunction using validated and dependable techniques...
December 12, 2017: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/29231782/akin10-activity-as-a-cellular-link-between-metabolism-and-circadian-clock-entrainment-in-arabidopsis-thaliana
#10
Alfredo Sánchez-Villarreal, Amanda M Davis, Seth J Davis
AKIN10, the catalytic subunit of the Snf1 (sucrose non-fermenting 1)-related kinase 1 (SnRK1) complex, acts as an energy sensor in plants. We showed that AKIN10-induced expression affects the pace of the circadian clock and particularly the phase of expression of GIGANTEA (GI). The AKIN10 effect on period length required TIME FOR COFFEE (TIC), a circadian-clock component with developmental and metabolic roles. Here we expand on the possible interactions between AKIN10, whose activity is involved in transcriptional reprogramming, and clock elements GI and TIC...
December 12, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29230818/relationship-between-carbohydrate-composition-and-fungal-deterioration-of-functional-strawberry-juices-preserved-using-non-thermal-treatments
#11
Lucía Cassani, Gabriel Quintana, María R Moreira, Andrea Gómez-Zavaglia
BACKGROUND: The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) were studied, as well as the evolution of these compounds and their relationship with fungal deterioration during 14 days of refrigerated storage. RESULTS: A simple and environmentally friendly analytical approach based on high performance liquid chromatography with reflection index detector was developed for simultaneous determination of inulin, FOS and mono and disaccharides present in the juices...
December 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29230167/changes-in-the-cholinergic-catecholaminergic-orexinergic-and-serotonergic-structures-forming-part-of-the-sleep-systems-of-adult-mice-exposed-to-intrauterine-alcohol
#12
Oladiran I Olateju, Adhil Bhagwandin, Amadi O Ihunwo, Paul R Manger
We examined the effect of chronic prenatal alcohol exposure on certain neuronal systems involved with the sleep-wake cycle of C57BL/6J mice exposed to prenatal alcohol once they had reached 56 days post-natal. Pregnant mice were exposed to alcohol, through oral gavage, on gestational days 7-16, with recorded blood alcohol concentration (BAC)s averaging 1.84 mg/ml (chronic alcohol group, CA). Two control groups, an oral gavage sucrose control group (chronic alcohol control group, CAc) and a non-treated control group (NTc), were also examined...
2017: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/29229561/effect-of-trehalose-and-sucrose-based-extenders-on-equine-sperm-quality-after-vitrification-preliminary-results
#13
C C Pérez-Marín, F D Requena, A Arando, S Ortiz-Villalón, F Requena, E I Agüera
There has been a lack of research into equine sperm vitrification to date, but studies of other species suggest it may have significant potential. To evaluate the impact of various cryoprotectant agents (CPA) and vitrification on equine sperm quality, a controlled study was carried out. A total of 12 ejaculates were subjected to exposure to CPA and vitrification. Sperm was diluted in a range of CPA: fresh, control (BSA), sucrose (0.15M, 0.3M and 0.5M), trehalose (0.15M, 0.3M and 0.5M) and the combination of sucrose and trehalose (M1: 0...
December 8, 2017: Cryobiology
https://www.readbyqxmd.com/read/29229337/receptor-protein-of-lysinibacillus-sphaericus-mosquito-larvicidal-toxin-displays-amylomaltase-activity
#14
Mahima Sharma, Gagan D Gupta, Vinay Kumar
The activated binary toxin (BinAB) from Lysinibacillus sphaericus binds to surface receptor protein (Cqm1) on the midgut cell membrane and kills Culex quinquefasciatus larvae on internalization. Cqm1 is attached to cells via a glycosyl-phosphatidylinositol (GPI) anchor. It has been classified as a member of glycoside hydrolase family 13 of the CAZy database. Here, we report characterization of the ordered domain (residues 23-560) of Cqm1. Gene expressing Cqm1 of BinAB susceptible mosquito was chemically synthesized and the protein was purified using E...
December 8, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29229334/broad-range-chemical-profiling-of-natural-deep-eutectic-solvent-extracts-using-a-high-performance-thin-layer-chromatography-based-method
#15
Xiaojie Liu, Samantha Ahlgren, Henrie A A J Korthout, Luis F Salomé-Abarca, Lina M Bayona, Robert Verpoorte, Young Hae Choi
Natural deep eutectic solvents (NADES) made mainly with abundant primary metabolites are being increasingly applied in green chemistry. The advantages of NADES as green solvents have led to their use in novel green products for the food, cosmetics and pharma markets. However, one of the main difficulties encountered in the development of novel products and their quality control arises from their low vapour pressure and high viscosity. These features create the need for the development of new analytical methods suited to this type of sample...
December 6, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29228731/impact-of-anger-emotional-stress-before-pregnancy-on-adult-male-offspring
#16
Sheng Wei, Xinyang Xiao, Jieqiong Wang, Shiguang Sun, Zifa Li, Kaiyong Xu, Fang Li, Jie Gao, Dehao Zhu, Mingqi Qiao
Previous studies have reported that maternal chronic stress or depression is linked to an increased risk of affective disorders in progeny. However, the impact of maternal chronic stress before pregnancy on the progeny of animal models is unknown. We investigated the behaviors and the neurobiology of 60-day-old male offspring of female rats subjected to 21 days of resident-intruder stress before pregnancy. An anger stressed parental rat model was established using the resident-intruder paradigm and it was evaluated using behavioral tests...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228399/effects-of-glucose-and-sucrose-on-mood-a-systematic-review-of-interventional-studies
#17
Ondine van de Rest, Nikita L van der Zwaluw, Lisette C P G M de Groot
Context: Glucose is the main energy source for the brain, and as such, manipulation of glucose supply may affect brain function. It has been suggested that a change in blood glucose may influence mood. Objective: The aim of this review was to investigate the potential effects of glucose and sucrose, compared with placebo, on mood. Data Sources: The electronic databases PubMed and Scopus were searched. Reference lists of selected articles were checked manually...
December 8, 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/29228351/defensive-behaviors-of-the-new-mealybug-citrus-pest-delottococcus-aberiae-hemiptera-pseudococcidae-against-three-generalist-parasitoids
#18
Alejandro Tena, Eliana Nieves, Julieta Herrero, Alberto Urbaneja
Delottococcus aberiae De Lotto (Hemiptera: Pseudococcidae) is an invasive mealybug that has become a citrus pest in Europe. This mealybug species causes serious damage because it deforms the fruits. Here, we studied the defensive behavior of D. aberiae when it was attacked by three parasitoid species: Acerophagus angustifrons (Gahan), Anagyrus sp. near pseudoccoci (Girault), and Leptomastix algirica Trjapitzin (Hymenoptera: Encyrtidae). Anagyrus sp. near pseudoccoci and L. algirica detected and accepted nymphs and adult females of D...
December 8, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29228055/transcriptional-profiling-of-sugarcane-leaves-and-roots-under-progressive-osmotic-stress-reveals-a-regulated-coordination-of-gene-expression-in-a-spatiotemporal-manner
#19
Alejandro Pereira-Santana, Edyciel J Alvarado-Robledo, Jesus A Zamora-Briseño, Jorge T Ayala-Sumuano, Victor M Gonzalez-Mendoza, Francisco Espadas-Gil, Luis D Alcaraz, Enrique Castaño, Miguel A Keb-Llanes, Felipe Sanchez-Teyer, Luis Carlos Rodriguez-Zapata
Sugarcane is one of the most important crops worldwide and is a key plant for the global production of sucrose. Sugarcane cultivation is severely affected by drought stress and it is considered as the major limiting factor for their productivity. In recent years, this plant has been subjected to intensive research focused on improving its resilience against water scarcity; particularly the molecular mechanisms in response to drought stress have become an underlying issue for its improvement. To better understand water stress and the molecular mechanisms we performed a de novo transcriptomic assembly of sugarcane (var...
2017: PloS One
https://www.readbyqxmd.com/read/29228046/genome-wide-microarray-analysis-leads-to-identification-of-genes-in-response-to-herbicide-metribuzin-in-wheat-leaves
#20
Whitney Pilcher, Hana Zandkamiri, Kelly Arceneaux, Stephen Harrison, Niranjan Baisakh
Herbicides are an important component of weed management in wheat, particularly in the southeastern US where weeds actively compete with wheat throughout the winter for nutrients and reduce tillering and ultimately the yield of the crop. Some wheat varieties are sensitive to metribuzin, a low-cost non-selective herbicide, leading to leaf chlorosis, stand loss, and decreased yield. Knowledge of the genetics of herbicide tolerance in wheat is very limited and most new varieties have not been screened for metribuzin tolerance...
2017: PloS One
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