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https://www.readbyqxmd.com/read/28735416/super-resolution-microscopical-localization-of-dopamine-receptors-1-and-2-in-rat-hippocampal-synaptosomes
#1
Andras G Miklosi, Giorgia Del Favero, Tanja Bulat, Harald Höger, Ryuichi Shigemoto, Doris Marko, Gert Lubec
Although dopamine receptors D1 and D2 play key roles in hippocampal function, their synaptic localization within the hippocampus has not been fully elucidated. In order to understand precise functions of pre- or postsynaptic dopamine receptors (DRs), the development of protocols to differentiate pre- and postsynaptic DRs is essential. So far, most studies on determination and quantification of DRs did not discriminate between subsynaptic localization. Therefore, the aim of the study was to generate a robust workflow for the localization of DRs...
July 22, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28735396/isolation-of-polysomal-rna-for-analyzing-stress-responsive-genes-regulated-at-the-translational-level-in-plants
#2
Yong-Fang Li, Ramamurthy Mahalingam, Ramanjulu Sunkar
Alteration of gene expression is an essential mechanism, which allows plants to respond and adapt to adverse environmental conditions. Transcriptome and proteome analyses in plants exposed to abiotic stresses revealed that protein levels are not correlated with the changes in corresponding mRNAs, indicating regulation at translational level is another major regulator for gene expression. Analysis of translatome, which refers to all mRNAs associated with ribosomes, thus has the potential to bridge the gap between transcriptome and proteome...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735061/up-regulation-of-glur1-in-paraventricular-nucleus-and-greater-expressions-of-synapse-related-proteins-in-the-hypothalamus-of-chronic-unpredictable-stress-induced-depressive-rats
#3
Rong Li, Xia Zhao, Li Cai, Wan-Wan Gao
Converging evidence indicates that abnormal glutamatergic and synaptic systems may be associated with the pathophysiology of depression. Over-activation of corticotropin-releasing hormone (CRH) neurons in the hypothalamic paraventricular nucleus (PVN) plays a key role in the hypothalamic-pituitary-adrenal (HPA) axis hyperactivity and is a prominent feature in depression. In this study, we examined the effect of chronic unpredictable stress (CUS) in rats on the expression of AMPA subunit GluR1 protein in the hypothalamic PVN as well as on the expression of GluR1 and several synapse-related proteins in the hypothalamus...
July 19, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28734224/structural-characterization-of-glucosylated-lactose-derivatives-synthesized-by-the-lactobacillus-reuteri-gtfa-and-gtf180-glucansucrase-enzymes
#4
Hien T T Pham, Lubbert Dijkhuizen, Sander S van Leeuwen
Glucansucrase enzymes from lactic acid bacteria are receiving strong interest because of their wide range of gluco-oligosaccharide and polysaccharide products from sucrose, some of which have prebiotic potential. Glucansucrases GtfA and Gtf180 from Lactobacillus reuteri strains are known to convert sucrose into α-glucans with different types of linkages, but also to use other molecules as acceptor substrates. Here we report that incubation of (N-terminally truncated versions of) these enzymes with lactose plus sucrose resulted in synthesis of at least 5 glucosylated lactose products of a degree of polymerization (DP) of 3-4...
July 8, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28733872/metabolic-profiles-of-flooding-tolerant-mechanism-in-early-stage-soybean-responding-to-initial-stress
#5
Xin Wang, Wei Zhu, Akiko Hashiguchi, Minoru Nishimura, Jingkui Tian, Setsuko Komatsu
Metabolomic analysis of flooding-tolerant mutant and abscisic acid-treated soybeans suggests that accumulated fructose might play a role in initial flooding tolerance through regulation of hexokinase and phosphofructokinase. Soybean is sensitive to flooding stress, which markedly reduces plant growth. To explore the mechanism underlying initial-flooding tolerance in soybean, mass spectrometry-based metabolomic analysis was performed using flooding-tolerant mutant and abscisic-acid treated soybeans. Among the commonly-identified metabolites in both flooding-tolerant materials, metabolites involved in carbohydrate and organic acid displayed same profile at initial-flooding stress...
July 21, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28733283/osmotic-compounds-enhance-antibiotic-efficacy-against-acinetobacter-baumannii-biofilm-communities
#6
Azeza Falghoush, Haluk Beyenal, Thomas E Besser, Anders Omsland, Douglas R Call
Biofilm-associated infections are a clinical challenge in part because a hydrated matrix protects the bacterial community from antibiotics. Herein, we evaluated how different osmotic compounds [maltodextrin, sucrose and polyethylene glycol (PEG)] enhance antibiotic efficacy against Acinetobacter baumannii biofilm communities. Established test-tube biofilms (24 h, strain ATCC 17978) were treated with osmotic compounds in the presence or absence of 10X the minimum inhibitory concentration of different antibiotics (50 μg/ml tobramycin, 20 μg/ml ciprofloxacin, 300 μg/ml chloramphenicol, 30 μg/ml nalidixic acid or 100 μg/ml erythromycin)...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28732144/influence-of-high-latitude-light-conditions-on-sensory-quality-and-contents-of-health-and-sensory-related-compounds-in-swede-roots-brassica-napus-l-ssp-rapifera-metzg
#7
Jørgen A B Mølmann, Sidsel Fiskaa Hagen, Gunnar B Bengtsson, Tor J Johansen
BACKGROUND: Vegetable growers in Arctic areas usually must rely on market strategies based on regional origin and product quality. Swede roots (rutabaga) were grown in a phytotron to investigate the effect of high latitude light conditions on sensory quality and some health and sensory-related compounds. Experimental treatments included modifications of 24 h natural day length (69° 39' N), by moving plants at daily intervals to dark chambers with either no light, fluorescent growth light and/or low intensity photoperiod extension...
July 21, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28730748/extracellular-signal-regulated-kinase-activation-in-the-spinal-cord-contributes-to-visceral-hypersensitivity-induced-by-craniofacial-injury-followed-by-stress
#8
Y-J Zhao, J-H Li, B Hu, Y Wang, X-F Chang, R J Traub, D-Y Cao
BACKGROUND: We previously developed an animal model to examine mechanisms that underlie the emergence of visceral hypersensitivity modeling pain characteristics of temporomandibular disorder (TMD) patients with comorbid irritable bowel syndrome (IBS). In ovariectomized (OVx) rats with estradiol (E2) replacement, visceral hypersensitivity developed subsequent to masseter muscle inflammation followed by repeated forced swim (FS) stress. The purpose of this study was to investigate whether activation of extracellular signal-regulated kinase (ERK) in the spinal cord contributes to visceral hypersensitivity in this overlapping pain model...
July 21, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28730325/single-stage-photofermentative-biohydrogen-production-from-sugar-beet-molasses-by-different-purple-non-sulfur-bacteria
#9
Emrah Sagir, Ebru Ozgur, Ufuk Gunduz, Inci Eroglu, Meral Yucel
Biohydrogen production via fermentative routes offers considerable advantages in waste recycling and sustainable energy production. This can be realized by single-stage dark or photofermentative processes, or by a two-stage integrated process; the latter offering the higher production yields due to complete conversion of sugar substrates into H2 and CO2. However, problems arising from the integration of these two processes limit its scale-up and implementation. Hence, high efficiency one-step fermentative biohydrogen production processes from sugar-rich wastes are preferable...
July 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28729389/severe-energy-deficit-up-regulates-leptin-receptors-leptin-signaling-and-ptp1b-in-human-skeletal-muscle
#10
Ismael Perez-Suarez, Jesus Gustavo Ponce-Gonzalez, Jaime de La Calle-Herrero, Jose Losa-Reyna, Marcos Martin-Rincon, David Morales-Alamo, Alfredo Santana, Hans-Christer Holmberg, Jose A L Calbet
In obesity, leptin receptors (OBR) and leptin signaling in skeletal muscle are down-regulated. To determine whether OBR and leptin signaling are up-regulated with a severe energy deficit fifteen overweight men were assessed before (PRE), after 4 days of caloric restriction (3.2 Kcal/kg BW/day) in combination with prolonged exercise (CRE) (8h walking + 45min single-arm cranking/day) to induce an energy deficit of ~5500 Kcal/d, and following 3 days of control diet (isoenergetic) and reduced exercise (CD). During CRE, the diet consisted solely of whey protein (n=8) or sucrose (n=7) (0...
July 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28729075/effects-of-environmental-enrichment-during-abstinence-in-morphine-dependent-parents-on-anxiety-depressive-like-behaviors-and-voluntary-morphine-consumption-in-rat-offspring
#11
Alireza Pooriamehr, Parviz Sabahi, Hossein Miladi-Gorji
Chronic morphine exposure during puberty increased morphine-induced rewarding effects and sensitization in the next generation. Given the well-known beneficial effects of environmental enrichment on the severity of physical and psychological dependence on morphine, we examined effects of enriched environment during morphine abstinence in morphine dependent parental rats before mating on the anxiety and depressive-like behaviors, and voluntary morphine consumption in their offspring. Paternal and/or maternal rats were injected with bi-daily doses (10mg/kg, 12h intervals) of morphine for 14days followed by rearing in a standard environment (SE) or enriched environment (EE) during 30days of morphine abstinence before mating...
July 17, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28729051/inhibiting-effects-of-fructanase-on-competence-stimulating-peptide-dependent-quorum-sensing-system-in-streptococcus-mutans
#12
Yusuke Suzuki, Ryo Nagasawa, Hidenobu Senpuku
Streptococcus mutans produces glucosyltransferases encoded by the gtfB and gtfC genes, which synthesize insoluble glucan, and both insoluble and soluble glucans by conversion of sucrose, and are known as principal agents to provide strong biofilm formation and demineralization on tooth surfaces. S. mutans possess a Com-dependent quorum sensing (QS) system, which is important for survival in severe conditions. The QS system is stimulated by the interaction between ComD {Receptor to competence-stimulating peptide (CSP)} encoded by the comD and CSP encoded by the comC, and importantly associated with bacteriocin production and genetic competence...
July 17, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28728372/supplementary-effects-of-higher-levels-of-various-disaccharides-on-processing-yield-quality-properties-and-sensory-attributes-of-chinese-style-pork-jerky
#13
Chih-Ming Chen, Hsien-Tang Lin
Objectives: This study was conducted to evaluate the supplementary effect of higher concentrations of various dissaccharides on processing yield, major physicochemical properties, and sensory attributes of Chinese-style pork jerky (CSPJ). Methods: CSPJ samples were prepared by marinating sliced ham (4 mm) with three dissaccharides, including sucrose, lactose, and maltose, at 0%, 15%, 18%, 21%, and 24%. Subsequently, the CSPJ samples were dried and roasted. The moisture content, water activity, crude protein, moisture-to-protein ratio, pH, processing yield, shear force, color, and sensory attributes of the CSPJ samples were evaluated...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728166/whole-genome-sequencing-revealed-novel-prognostic-biomarkers-and-promising-targets-for-therapy-of-ovarian-clear-cell-carcinoma
#14
Hiroaki Itamochi, Tetsuro Oishi, Nao Oumi, Satoshi Takeuchi, Kosuke Yoshihara, Mikio Mikami, Nobuo Yaegashi, Yasuhisa Terao, Kazuhiro Takehara, Kimio Ushijima, Hidemichi Watari, Daisuke Aoki, Tadashi Kimura, Toshiaki Nakamura, Yoshihito Yokoyama, Junzo Kigawa, Toru Sugiyama
BACKGROUND: Ovarian clear cell carcinoma (OCCC) is mostly resistant to standard chemotherapy that results in poor patient survival. To understand the genetic background of these tumours, we performed whole-genome sequencing of OCCC tumours. METHODS: Tumour tissue samples and matched blood samples were obtained from 55 Japanese women diagnosed with OCCC. Whole-genome sequencing was performed using the Illumina HiSeq platform according to standard protocols. RESULTS: Alterations to the switch/sucrose non-fermentable (SWI/SNF) subunit, the phosphatidylinositol-3-kinase (PI3K)/Akt signalling pathway, and the receptor tyrosine kinase (RTK)/Ras signalling pathway were found in 51%, 42%, and 29% of OCCC tumours, respectively...
July 20, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28728145/extracellular-dna-contributes-to-dental-biofilm-stability
#15
Sebastian Schlafer, Rikke L Meyer, Irene Dige, Viduthalai R Regina
Extracellular DNA (eDNA) is a major matrix component of many bacterial biofilms. While the presence of eDNA and its role in biofilm stability have been demonstrated for several laboratory biofilms of oral bacteria, there is no data available on the presence and function of eDNA in in vivo grown dental biofilms. This study aimed to determine whether eDNA was part of the matrix in biofilms grown in situ in the absence of sucrose and whether treatment with DNase dispersed biofilms grown for 2.5, 5, 7.5, 16.5, or 24 h...
July 21, 2017: Caries Research
https://www.readbyqxmd.com/read/28727494/efficacy-of-standard-prophylaxis-versus-on-demand-treatment-with-bayer-s-sucrose-formulated-recombinant-fviii-rfviii-fs-in-chinese-children-with-severe-hemophilia-a
#16
Yongqiang Zhao, Juan Xiao, Renchi Yang, Runhui Wu, Yu Hu, Horst Beckmann, Junde Wu, Qingsong Hou, Jing Sun
In China, care of patients with severe hemophilia primarily involves insufficient dosing of on-demand treatment and secondary low-dose prophylaxis (10 IU/kg 2× /wk). We sought to evaluate 3× /wk, standard-dose prophylaxis with sucrose-formulated recombinant factor VIII (rFVIII-FS; Bayer) compared with on-demand treatment in Chinese children with severe hemophilia A. Children and adolescents aged 2-16 years with severe hemophilia A, no inhibitors, and no prophylaxis for >6 consecutive months before study entry were eligible for this 24-week, interventional, sequential-treatment study...
July 20, 2017: Pediatric Hematology and Oncology
https://www.readbyqxmd.com/read/28726801/accumbens-mechanisms-for-cued-sucrose-seeking
#17
Ana-Clara Bobadilla, Constanza Garcia-Keller, Jasper A Heinsbroek, Michael Scofield, Victoria Chareunsouk, Cara Monforton, Peter W Kalivas
Many studies support a perspective that addictive drugs usurp brain circuits used by natural rewards, especially for the dopamine-dependent reinforcing qualities of both drugs and natural rewards. Reinstated drug seeking in animal models of relapse relies on glutamate spillover from cortical terminals synapsing in the nucleus accumbens core (NAcore) to stimulate metabotropic glutamate receptor5 (mGluR5) on neuronal nitric oxide synthase (nNOS) interneurons. Contrasting the release of dopamine that is shared by sucrose and drugs of abuse, reinstated sucrose seeking does not induce glutamate spillover...
July 20, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28725961/the-plasma-membrane-proteome-of-medicago-truncatula-roots-as-modified-by-arbuscular-mycorrhizal-symbiosis
#18
Achref Aloui, Ghislaine Recorbet, Christelle Lemaître-Guillier, Arnaud Mounier, Thierry Balliau, Michel Zivy, Daniel Wipf, Eliane Dumas-Gaudot
In arbuscular mycorrhizal (AM) roots, the plasma membrane (PM) of the host plant is involved in all developmental stages of the symbiotic interaction, from initial recognition to intracellular accommodation of intra-radical hyphae and arbuscules. Although the role of the PM as the agent for cellular morphogenesis and nutrient exchange is especially accentuated in endosymbiosis, very little is known regarding the PM protein composition of mycorrhizal roots. To obtain a global overview at the proteome level of the host PM proteins as modified by symbiosis, we performed a comparative protein profiling of PM fractions from Medicago truncatula roots either inoculated or not with the AM fungus Rhizophagus irregularis...
July 19, 2017: Mycorrhiza
https://www.readbyqxmd.com/read/28725638/isolation-of-f-novicida-containing-phagosome-from-infected-human-monocyte-derived-macrophages
#19
Valentina Marecic, Olga Shevchuk, Mateja Ozanic, Mirna Mihelcic, Michael Steinert, Antonija Jurak Begonja, Yousef Abu Kwaik, Marina Santic
Francisella is a gram-negative bacterial pathogen, which causes tularemia in humans and animals. A crucial step of Francisella infection is its invasion of macrophage cells. Biogenesis of the Francisella-containing phagosome (FCP) is arrested for ~15 min at the endosomal stage, followed by gradual bacterial escape into the cytosol, where the microbe proliferates. The crucial step in pathogenesis of tularemia is short and transient presence of the bacterium within phagosome. Isolation of FCPs for further studies has been challenging due to the short period of time of bacterial residence in it and the characteristics of the FCP...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28725337/complete-genome-sequence-of-leuconostoc-suionicum-dsm-20241-t-provides-insights-into-its-functional-and-metabolic-features
#20
Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon
The genome of Leuconostoc suionicum DSM 20241(T) (=ATCC 9135(T) = LMG 8159(T) = NCIMB 6992(T)) was completely sequenced and its fermentative metabolic pathways were reconstructed to investigate the fermentative properties and metabolites of strain DSM 20241(T) during fermentation. The genome of L. suionicum DSM 20241(T) consists of a circular chromosome (2026.8 Kb) and a circular plasmid (21.9 Kb) with 37.58% G + C content, encoding 997 proteins, 12 rRNAs, and 72 tRNAs. Analysis of the metabolic pathways of L...
2017: Standards in Genomic Sciences
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