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https://www.readbyqxmd.com/read/28931084/barcoding-snakeheads-teleostei-channidae-revisited-discovering-greater-species-diversity-and-resolving-perpetuated-taxonomic-confusions
#1
Cecilia Conte-Grand, Ralf Britz, Neelesh Dahanukar, Rajeev Raghavan, Rohan Pethiyagoda, Heok Hui Tan, Renny K Hadiaty, Norsham S Yaakob, Lukas Rüber
Snakehead fishes of the family Channidae are predatory freshwater teleosts from Africa and Asia comprising 38 valid species. Snakeheads are important food fishes (aquaculture, live food trade) and have been introduced widely with several species becoming highly invasive. A channid barcode library was recently assembled by Serrao and co-workers to better detect and identify potential and established invasive snakehead species outside their native range. Comparing our own recent phylogenetic results of this taxonomically confusing group with those previously reported revealed several inconsistencies that prompted us to expand and improve on previous studies...
2017: PloS One
https://www.readbyqxmd.com/read/28931050/saxs-and-stability-studies-of-iron-induced-oligomers-of-bacterial-frataxin-cyay
#2
Mostafa Fekry, Wessen Alshokry, Przemysław Grela, Marek Tchórzewski, Eva-Christina Ahlgren, Christopher A Söderberg, Oleksandr Gakh, Grazia Isaya, Salam Al-Karadaghi
Frataxin is a highly conserved protein found in both prokaryotes and eukaryotes. It is involved in several central functions in cells, which include iron delivery to biochemical processes, such as heme synthesis, assembly of iron-sulfur clusters (ISC), storage of surplus iron in conditions of iron overload, and repair of ISC in aconitase. Frataxin from different organisms has been shown to undergo iron-dependent oligomerization. At least two different classes of oligomers, with different modes of oligomer packing and stabilization, have been identified...
2017: PloS One
https://www.readbyqxmd.com/read/28930995/stereotactic-atlas-guided-laser-capture-microdissection-of-brain-regions-affected-by-traumatic-injury
#3
Harris A Weisz, Deborah R Boone, Stacy L Sell, Helen L Hellmich
The ability to isolate specific brain regions of interest can be impeded in tissue disassociation techniques that do not preserve their spatial distribution. Such techniques also potentially skew gene expression analysis because the process itself can alter expression patterns in individual cells. Here we describe a laser capture microdissection (LCM) method to selectively collect specific brain regions affected by traumatic brain injury (TBI) by using a modified Nissl (cresyl violet) staining protocol and the guidance of a rat brain atlas...
September 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#4
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930522/advances-in-tick-vaccinology-in-brazil-from-gene-expression-to-immunoprotection
#5
Renato Andreotti, Poliana Fernanda Giachetto, Rodrigo Casquero Cunha
Rhipicephalus (Boophilus) microplus has substantial economic impact on the cattle breeding industry and, chemical control and tick resistance development are the major concern. There is a worldwide search for new options, and control using vaccines has been the main focus nowadays. Studies performed in Brazil found that Bm86-based immunization of bovines reduced the infestation of R. (B.) microplus of vaccinated bovines by 45% to 60%. Native Boophilusmicroplus tripsin inhibitors (BmTIs) with trypsin-, kallikrein-, and elastase-inhibiting activities have been used as immunogens in bovines reaching 72...
January 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28930438/supported-planar-mammalian-membranes-as-models-of-in-vivo-cell-surface-architectures
#6
Han-Yuan Liu, Hannah Grant, Hung-Lun Hsu, Raya Sorkin, Filip Bošković, G J L Wuite, Susan Daniel
Emerging technologies use cell plasma membrane vesicles or 'blebs' as an intermediate to form molecularly complete, planar cell surface mimetics that are compatible with a variety of characterization tools and microscopy methods. This approach enables direct incorporation of membrane proteins into supported lipid bilayers without using detergents and reconstitution and preserves native lipids and membrane species. Such a system can be advantageous as in vitro models of in vivo cell surfaces for study of the roles of membrane proteins as drug targets in drug delivery, host-pathogen interactions, tissue engineering, and many other bioanalytical and sensing applications...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28930432/injectable-stem-cell-laden-open-porous-microgels-that-favour-adipogenesis-in-vitro-and-in-vivo-evaluation
#7
Pengfei Xia, Kunxi Zhang, Yan Gong, Guifei Li, Shifeng Yan, Jingbo Yin
Microgels, with large surface area per volume, show great advantages in adipose tissue engineering due to their injectability and similarity with natural extracellular matrix. However, to date, no studies have tried applying microgels to adipose tissue regeneration. Herein, based on double bonded poly-(L-glutamic acid)-g-2-Hydroxyethyl methacrylate (PLGA-g-HEMA) and maleic anhydride-modified chitosan (MCS), an open porous microgel with high hydrophilicity and great injectability is successfully prepared (microgels diameter of 200-300 μm, pore diameter of 38 μm, and porosity of 88...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28930289/genetic-signatures-of-variation-in-population-size-in-a-native-fungal-pathogen-after-the-recent-massive-plantation-of-its-host-tree
#8
F Labbé, M C Fontaine, C Robin, C Dutech
Historical fluctuations in forests' distribution driven by past climate changes and anthropogenic activities can have large impacts on the demographic history of pathogens that have a long co-evolution history with these host trees. Using a population genetic approach, we investigated that hypothesis by reconstructing the demographic history of Armillaria ostoyae, one of the major pathogens of the maritime pine (Pinus pinaster), in the largest monospecific pine planted forest in Europe (south-western France)...
September 20, 2017: Heredity
https://www.readbyqxmd.com/read/28930280/chemical-composition-and-monoterpenoid-enantiomeric-distribution-of-the-essential-oils-from-apharsemon-commiphora-gileadensis
#9
Nativ Dudai, Alona Shachter, Prabodh Satyal, William N Setzer
Background:Commiphora gileadensis (Hebrew: apharsemon) has been used since Biblical times to treat various ailments, and is used today in the traditional medicine of some Middle Eastern cultures. Methods: The essential oils from the stem bark, leaves, and fruits of Commiphora gileadensis-collected at the Ein Gedi Botanical Garden, Israel-were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. In addition, the enantiomeric distributions of the monoterpenoids in the essential oils have been determined by chiral gas chromatography...
September 12, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930265/a-review-on-ethno-medicinal-and-pharmacological-activities-of-sphenocentrum-jollyanum-pierre
#10
REVIEW
Olubukola Sinbad Olorunnisola, Olumide Samuel Fadahunsi, Peter Adegbola
Sphenocentrum jollyanum Pierre is a member of a diverse family of plants known as Menispermaceae. They are famous for a plethora of important biological functions. S. jollyanum is a shrub native to the tropical forest zones of West Africa and thrives in deep shade. It is widely cultivated in Cameroun, Sierra Leone, Nigeria, Ghana, and Côte d'Ivoire. S. jollyanum is employed in folk medicine as a cure for wounds, fever, coughs, high blood pressure, breast tumor, constipation, and as an aphrodisiac. Phytochemical investigations revealed that the plant is a rich source of secondary metabolites such as annin, alkaloids, saponins, and flavonoids...
July 3, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930241/chemical-composition-antimicrobial-and-antioxidant-activities-of-essential-oils-from-two-avicennia-schaueriana-stapf-leechm-ex-moldenke-acanthaceae-populations
#11
Kamilla N Machado, Telma M Kaneko, Maria Cláudia M Young, Cynthia Murakami, Inês Cordeiro, Paulo Roberto H Moreno
BACKGROUND: Avicennia schaueriana Stapf & Leechm. ex Moldenke (Acanthaceae) is a native species from the Brazilian mangroves presenting ecological and economic significance. This study compared the composition and the biological activities from the essential oils obtained from two A. schaueriana populations collected at Jureia-Itatins and Ilha do Cardoso. METHODS: Essential oils were obtained by conventional means, and their compositions were analyzed by GC-MS...
May 1, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930158/a-method-for-medical-diagnosis-based-on-optical-fluence-rate-distribution-at-tissue-surface
#12
Omnia Hamdy, Jala El-Azab, Tarek A Al-Saeed, Mahmoud F Hassan, Nahed H Solouma
Optical differentiation is a promising tool in biomedical diagnosis mainly because of its safety. The optical parameters' values of biological tissues differ according to the histopathology of the tissue and hence could be used for differentiation. The optical fluence rate distribution on tissue boundaries depends on the optical parameters. So, providing image displays of such distributions can provide a visual means of biomedical diagnosis. In this work, an experimental setup was implemented to measure the spatially-resolved steady state diffuse reflectance and transmittance of native and coagulated chicken liver and native and boiled breast chicken skin at 635 and 808 nm wavelengths laser irradiation...
September 20, 2017: Materials
https://www.readbyqxmd.com/read/28930094/effect-of-surface-roughness-chemical-composition-and-native-oxide-crystallinity-on-the-orientation-of-self-assembled-gan-nanowires-on-ti-foils
#13
G Calabrese, S V Pettersen, C Pfüller, M Ramsteiner, J K Grepstad, O Brandt, L Geelhaar, S Fernández-Garrido
We report on plasma-assisted molecular beam epitaxial growth of almost randomly oriented, uniformly tilted, and vertically aligned self-assembled GaN nanowires (NWs), respectively, on different types of polycrystalline Ti foils. The NW orientation with respect to the substrate normal, which is affected by an in situ treatment of the foil surface before NW growth, depends on the crystallinity of the native oxide. Direct growth on the as-received foils results in the formation of ensembles of nearly randomly oriented NWs due to the strong roughening of the surface induced by chemical reactions between the impinging elements and Ti...
September 20, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28930090/biomimetic-matrix-fabricated-by-lmp-1-gene-transduced-mc3t3-e1-cells-for-bone-regeneration
#14
Junxuan Ma, Wei Guo, Manman Gao, Baoding Huang, Qihua Qi, Zemin Ling, Yan Chen, Hao Hu, Hailian Zhou, Fei Yu, Ke Chen, Zhiyu Zhou, Deming Xiao, Xuenong Zou
Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). The study aimed to mimic native osteoinductive microenvironment by developing an ECM using gene-transduced cells. LIM mineralization protein-1 (LMP-1) gene was transferred to the murine pre-osteoblast cells (MC3T3-E1) using lentiviral vectors. Western blotting essay indicated that the MC3T3-E1 cells expressed an increased level of bone morphologic protein-2, -4 and-7 (BMP-2, -4 and -7) after LMP-1 gene transduction...
September 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28930080/seroprevalence-of-brucella-antibodies-in-harbor-seals-in-alaska-usa-with-age-regional-and-reproductive-comparisons
#15
A Hoover-Miller, J L Dunn, C L Field, G Blundell, S Atkinson
Populations of harbor seal Phoca vitulina in the Gulf of Alaska have dramatically declined during the past 4 decades. Numbers of seals in Glacier Bay, in southeast Alaska, USA, have also declined despite extensive protection. Causes of the declines and slow recovery are poorly understood. Brucellosis is a zoonotic disease that adversely affects reproduction in many domestic species. We measured the seroprevalence of Brucella antibodies in 554 harbor seals in 3 Alaska locations: Prince William Sound (PWS), Glacier Bay (GB), and Tracy Arm Fords Terror (TAFT) Wilderness Area...
September 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28930049/experience-with-the-syncardia-total-artificial-heart-in-a-canadian-centre
#16
Anthony Nguyen, Michel Pellerin, Louis P Perrault, Michel White, Anique Ducharme, Normand Racine, Michel Carrier
BACKGROUND: The SynCardia total artificial heart (TAH) provides complete circulatory support by replacing both native ventricles. Accepted indications include bridge to transplantation and destination therapy. We review our experience with TAH implantation during a period when axial flow pump became available. METHODS: We retrospectively analyzed the demographics, clinical characteristics and survival of all patients receiving the TAH. RESULTS: From September 2004 to November 2016, 13 patients (12 men, mean age 45 ± 13 yr) received the TAH for refractory cardiogenic shock secondary to idiopathic (56%) or ischemic (17%) cardiomyopathy and to other various causes (33%)...
October 1, 2017: Canadian Journal of Surgery. Journal Canadien de Chirurgie
https://www.readbyqxmd.com/read/28929425/medium-term-outcomes-after-reversal-of-roux-en-y-gastric-bypass
#17
Gustavo Andrés Arman, J Himpens, R Bolckmans, D Van Compernolle, R Vilallonga, G Leman
BACKGROUND: Roux-en-Y gastric bypass (RYGB) can be reversed into normal anatomy (NA) or into sleeve gastrectomy (NASG) to address undesired side effects. Concomitant hiatal hernia repair (HHR) may be required. Before reversal, some patients benefit from placement of a gastrostomy, mostly to predict the result of recreating the native anatomy. METHODS: Retrospective study on mid-term effects of RYGB reversal to NA and NASG, including clinical and weight evolution, surgical complications, and incidence of gastro-esophageal reflux (GERD)...
September 19, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28929399/prioritisation-of-native-legume-species-for-further-evaluation-as-potential-forage-crops-in-water-limited-agricultural-systems-in-south-africa
#18
Francuois L Müller, Lincoln M Raitt, Samson B M Chimphango, M Igshaan Samuels, Clement F Cupido, J Stephen Boatwright, Richard Knight, Marike Trytsman
In the face of climate change, identification of forage species suitable for dryland farming under low rainfall conditions in South Africa is needed. Currently, there are only a limited number of forage species suitable for dryland farming under such conditions. The objective of this study was to identify and prioritise native legume species that could potentially be used in dryland farming systems in water-limited agro-ecosystems in South Africa. Using a combination of ecological niche modelling techniques, plant functional traits, and indigenous knowledge, 18 perennial herbaceous or stem-woody legume species were prioritised for further evaluation as potential fodder species within water-limited agricultural areas...
September 19, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28929266/brazilian-meningococcal-c-conjugate-vaccine-physicochemical-immunological-and-thermal-stability-characteristics
#19
Renata Chagas Bastos, Marilza Batista Corrêa, Iaralice Medeiros de Souza, Milton Neto da Silva, Denise da Silva Gomes Pereira, Fernanda Otaviano Martins, Camila da Silva Faria, Ana Paula Dinis Ano Bom, Maria de Lourdes Leal, Ellen Jessouroun, José Godinho da Silva, Ricardo de Andrade Medronho, Ivna Alana Freitas Brasileiro da Silveira
High temperature is known to cause some instability in polysaccharide-protein conjugated vaccines and studies under stress conditions may be useful in determining whether short-term accidental exposure to undesired conditions can compromise product quality. In this study, we examined the structural stability of three industrial batches of Brazilian Meningococcal C conjugate bulk (MPCT) incubated at 4, 37, and 55 °C for 5 weeks. The effect of exposure to the storage temperatures was monitored by HPLC-SEC, CZE, CD and NMR techniques...
September 19, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28929253/positive-rheotaxis-extended-drop-a-one-step-procedure-to-select-and-recover-sperm-with-mature-chromatin-for-intracytoplasmic-sperm-injection
#20
Hamilton De Martin, Marcello S Cocuzza, Bruno C Tiseo, Guilherme J A Wood, Eduardo P Miranda, Pedro A A Monteleone, José Maria Soares, Paulo C Serafini, Miguel Srougi, Edmund C Baracat
PURPOSE: The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method. METHODS: This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS)...
September 19, 2017: Journal of Assisted Reproduction and Genetics
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