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https://www.readbyqxmd.com/read/28738566/preparation-and-evaluation-of-a-novel-n-benzyl-phenethylamino-%C3%AE-cyclodextrin-bonded-chiral-stationary-phase-for-hplc
#1
Liang Li, Biaoping Cheng, Rendan Zhou, Zhigang Cao, Chun Zeng, Laisheng Li
A novel N-benzyl-phenethylamino-β-cyclodextrin-bonded ordered mesoporous SBA-15 chiral stationary phase (BZCDP) for high-performance liquid chromatography (HPLC) was prepared. The structure and morphology of the ligand and the stationary phase were characterized by mass spectrometry, elemental analysis, infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis. The enantiomers of chiral compounds including nine common β-adrenergic blocker drugs (β-blockers), eight dansyl amino acids (DNS-AA) and six flavanones were successfully separated by polar organic solvent and reversed-phase chromatography, respectively...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738307/pharmacological-characterization-and-binding-modes-of-novel-racemic-and-optically-active-phenylalanine-based-antagonists-of-ampa-receptors
#2
Ewa Szymańska, Birgitte Nielsen, Tommy N Johansen, Ana Maria Cuñado Moral, Darryl S Pickering, Katarzyna Szczepańska, Anna Mickowska, Katarzyna Kieć-Kononowicz
In order to map out molecular determinants for the competitive blockade of AMPA receptor subtypes, a series of racemic aryl-substituted phenylalanines was synthesized and pharmacologically characterized in vitro at native rat ionotropic glutamate receptors. Most of the compounds showed micromolar affinity and preference for AMPA receptors. Individual stereoisomers of selected compounds were further evaluated at recombinant homomeric rat GluA2 and GluA3 receptors. The most potent compound, (-)-2-amino-3-(6-chloro-2',5'-dihydroxy-5-nitro-[1,1'-biphenyl]-3-yl)propanoic acid, the expected R-isomer showing Ki of 1...
July 8, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28738087/exercise-physiology-with-a-left-ventricular-assist-device-analysis-of-heart-pump-interaction-with-a-computational-simulator
#3
Libera Fresiello, Frank Rademakers, Piet Claus, Gianfranco Ferrari, Arianna Di Molfetta, Bart Meyns
Patients with a Ventricular Assist Device (VAD) are hemodynamically stable but show an impaired exercise capacity. Aim of this work is to identify and to describe the limiting factors of exercise physiology with a VAD. We searched for data concerning exercise in heart failure condition and after VAD implantation from the literature. Data were analyzed by using a cardiorespiratory simulator that worked as a collector of inputs coming from different papers. As a preliminary step the simulator was used to reproduce the evolution of hemodynamics from rest to peak exercise (ergometer cycling) in heart failure condition...
2017: PloS One
https://www.readbyqxmd.com/read/28738069/inoculation-effects-on-root-colonizing-arbuscular-mycorrhizal-fungal-communities-spread-beyond-directly-inoculated-plants
#4
Martina Janoušková, Karol Krak, Miroslav Vosátka, David Püschel, Helena Štorchová
Inoculation with arbuscular mycorrhizal fungi (AMF) may improve plant performance at disturbed sites, but inoculation may also suppress root colonization by native AMF and decrease the diversity of the root-colonizing AMF community. This has been shown for the roots of directly inoculated plants, but little is known about the stability of inoculation effects, and to which degree the inoculant and the inoculation-induced changes in AMF community composition spread into newly emerging seedlings that were not in direct contact with the introduced propagules...
2017: PloS One
https://www.readbyqxmd.com/read/28738015/observational-study-to-estimate-the-changes-in-the-effectiveness-of-bacillus-calmette-gu%C3%A3-rin-bcg-vaccination-with-time-since-vaccination-for-preventing-tuberculosis-in-the-uk
#5
Punam Mangtani, Patrick Nguipdop-Djomo, Ruth H Keogh, Lucy Trinder, Peter G Smith, Paul Em Fine, Jonathan Sterne, Ibrahim Abubakar, Emilia Vynnycky, John Watson, David Elliman, Marc Lipman, Laura C Rodrigues
BACKGROUND: Until recently, evidence that protection from the bacillus Calmette-Guérin (BCG) vaccination lasted beyond 10 years was limited. In the past few years, studies in Brazil and the USA (in Native Americans) have suggested that protection from BCG vaccination against tuberculosis (TB) in childhood can last for several decades. The UK's universal school-age BCG vaccination programme was stopped in 2005 and the programme of selective vaccination of high-risk (usually ethnic minority) infants was enhanced...
July 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28737981/contact-forces-acting-on-large-femoral-osteochondral-allografts-during-forced-knee-extension
#6
Peter Z Du, Keith L Markolf, Christopher J Lama, David R McAllister, Kristofer J Jones
BACKGROUND: A single cylindrical graft plug is commonly used for large focal femoral defects during osteochondral allograft (OCA) transplantation. Excessive contact force (CF) on a proud plug could compromise initial healing. CFs during forced knee extension are of particular interest because this maneuver is used by therapists to restore early postoperative range of motion. HYPOTHESIS: A proud OCA plug will significantly increase the CF and significantly decrease the knee extension angle (KEA)...
July 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28737980/nitration-of-h2b-histone-elicits-an-immune-response-in-experimental-animals
#7
Md Asad Khan, Khursheed Alam, Syed Mehdi Hassan, M Moshahid A Rizvi
Histone H2B is an autoantigen that appears in circulation due to altered apoptosis/or insufficient clearance and is likely to be involved in the induction and progression of autoimmune diseases since modified-H2B is immunogenic. Our studies demonstrate that tyrosines of H2B histone spontaneously converts to free and nitrotyrosine bound protein in vivo. Commercially available H2B histone was modified with peroxynitrite in vitro. Modified H2B was found to be more immunogenic than native form in experimental animals...
July 24, 2017: Autoimmunity
https://www.readbyqxmd.com/read/28737967/integral-membrane-proteins-bottom-up-top-down-and-structural-proteomics
#8
Upendra Karr, Margaret Simonian, Julian P Whitelegge
Integral membrane proteins and lipids constitute the bilayer membranes that surround cells and sub-cellular compartments, and modulate movements of molecules and information between them. Since membrane protein drug targets represent a disproportionately large segment of the proteome, technical developments need timely review. Areas covered and literature search strategy: Publically available resources such as Pubmed were surveyed. Bottom-up proteomics analyses now allow efficient extraction and digestion such that membrane protein coverage is essentially complete, making up around one third of the proteome...
July 24, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28737929/enhanced-degradation-of-tce-on-a-superfund-site-using-endophyte-assisted-poplar-tree-phytoremediation
#9
Sharon Lafferty Doty, John Leonard Freeman, Christopher M Cohu, Joel Gerard Burken, Andrea Firrincieli, Andrew Simon, Zareen Khan, Jud G Isebrands, Joseph Lukas, Michael J Blaylock
Trichloroethylene (TCE) is a widespread environmental pollutant common in groundwater plumes associated with industrial manufacturing areas. We had previously isolated and characterized a natural bacterial endophyte, Enterobacter sp. strain PDN3, of poplar trees, that rapidly metabolizes TCE, releasing chloride ion. We now report findings from a successful three-year field trial of endophyte-assisted phytoremediation on the Middlefield-Ellis-Whisman Superfund Study Area TCE plume in the Silicon Valley of California...
July 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28737752/mechanical-strain-sensing-implicated-in-cell-shape-recovery-in-escherichia-coli
#10
Felix Wong, Lars D Renner, Gizem Özbaykal, Jayson Paulose, Douglas B Weibel, Sven van Teeffelen, Ariel Amir
The shapes of most bacteria are imparted by the structures of their peptidoglycan cell walls, which are determined by many dynamic processes that can be described on various length scales ranging from short-range glycan insertions to cellular-scale elasticity(1-11). Understanding the mechanisms that maintain stable, rod-like morphologies in certain bacteria has proved to be challenging due to an incomplete understanding of the feedback between growth and the elastic and geometric properties of the cell wall(3,4,12-14)...
July 24, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28737731/study-on-chemical-profile-and-neuroprotective-activity-of-myrica-rubra-leaf-extract
#11
Pinghong Chen, Xianzong Lin, Ching-Hsu Yang, Xu Tang, Yu-Wei Chang, Weibing Zheng, Lianzhong Luo, Changan Xu, Yung-Husan Chen
The chemical profile of Myrica rubra (a native species in China) leaf extract was investigated by UPLC-PDA-HRMS, and the neuroprotective activity of two characteristic constituents, myricanol and myricetrin, was evaluated with N2a cells using H₂O₂-inducedoxidative challenge through a series of methods, e.g., MTT assay, ROS assay and [Ca(2+)]i assay. Among the 188 constituents detected in the extract of Myrica rubra leaf, 116 were identified definitely or tentatively by the comprehensive utilization of precise molecular weight and abundant multistage fragmentation information obtained by quadrupole orbitrap mass spectrometry...
July 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737286/unexpected-chiro-thermoresponsive-behavior-of-helical-poly-phenylacetylene-s-bearing-elastin-based-side-chains
#12
Sandra Arias, Felix Freire, Marcelo Calderon, Julian Bergueiro
The thermoresponsive behavior of an elastin based polymer can be altered by the polymeric macromolecular conformation. Thus, when the elastin basic amino-acid sequence VPGVG is used as a pendant group of a poly(phenylacetylene) (PPA) its thermoresponsivity in water can be remotely detected due to conformational changes on the formed helix. Circular dichroism at different temperatures show an inversion of the first Cotton effect (450 nm) at 25.8 ºC that matches with the cloud point temperature. Interestingly, this elastin-based side-chain PPA shows an upper critical solution temperature with low pH and concentration dependency, not expected in elastin based polymers...
July 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28737278/multicellular-vascularized-engineered-tissues-through-user-programmable-biomaterial-photodegradation
#13
Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
A photodegradable material-based approach to generate endothelialized 3D vascular networks within cell-laden hydrogel biomaterials is introduced. Exploiting multiphoton lithography, microchannel networks spanning nearly all size scales of native human vasculature are readily generated with unprecedented user-defined 4D control. Intraluminal channel architectures of synthetic vessels are fully customizable, providing new opportunities for next-generation microfluidics and directed cell function.
July 24, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28737038/evolution-of-aggregate-structure-in-solutions-of-anionic-monorhamnolipids-experimental-and-computational-results
#14
Ryan J Eismin, Elango Munusamy, Laurel L Kegel, David E Hogan, Raina M Maier, Steven D Schwartz, Jeanne E Pemberton
The evolution of solution aggregates of the anionic form of the native monorhamnolipid (mRL) mixture produced by Pseudomonas aeruginosa ATCC 9027 is explored at pH 8.0 using both experimental and computational approaches. Experiments utilizing surface tension measurements, dynamic light scattering, and both steady-state and time-resolved fluorescence spectroscopy reveal solution aggregation properties. All-atom molecular dynamics simulations on self-assemblies of the most abundant monorhamnolipid molecule, l-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-C10-C10), in its anionic state explore the formation of aggregates and the role of hydrogen bonding, substantiating the experimental results...
July 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28737018/increased-myocardial-native-t1-relaxation-time-in-patients-with-nonischemic-dilated-cardiomyopathy-with-complex-ventricular-arrhythmia
#15
Shiro Nakamori, An H Bui, Jihye Jang, Hossam A El-Rewaidy, Shingo Kato, Long H Ngo, Mark E Josephson, Warren J Manning, Reza Nezafat
PURPOSE: To study the relationship between diffuse myocardial fibrosis and complex ventricular arrhythmias (ComVA) in patients with nonischemic dilated cardiomyopathy (NICM). We hypothesized that NICM patients with ComVA would have a higher native myocardial T1 time, suggesting more extensive myocardial diffuse fibrosis. MATERIALS AND METHODS: We prospectively enrolled NICM patients with a history of ComVA (n = 50) and age-matched NICM patients without ComVA (n = 57)...
July 24, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28736993/molecular-characterization-of-streptococcus-pneumoniae-particularly-serotype19a-st320-that-emerged-in-krasnoyarsk-russia
#16
Irina N Protasova, Tsai-Wen Wan, Natalya V Bakhareva, Wei-Chun Hung, Wataru Higuchi, Yasuhisa Iwao, Tatyana A Yelistratova, Natalya A Ilyenkova, Yelena S Sokolovskaya, Galina P Martynova, Ivan V Reva, Galina V Reva, Sergey V Sidorenko, Lee-Jene Teng, Olga V Peryanova, Alla B Salmina, Tatsuo Yamamoto
Streptococcus pneumoniae, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). We herein report pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk). Analyses included antimicrobial susceptibility testing, ST typing, a pulsed-field gel electrophoresis analysis, virulence-related surface protein gene (VSG) typing with novel primers, and a structural analysis by scanning electron microscopy...
July 23, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28736891/heat-induced-native-dimerization-prevents-amyloid-formation-by-variable-domain-from-immunoglobulin-light-chain-rei
#17
Marina Nawata, Hirotaka Tsutsumi, Yuta Kobayashi, Satoru Unzai, Shouhei Mine, Tsutomu Nakamura, Koichi Uegaki, Hironari Kamikubo, Mikio Kataoka, Daizo Hamada
Amyloid light chain (AL) amyloidosis is a protein-misfolding disease characterized by accumulation of immunoglobulin light chains (LCs) into amyloid fibrils. Dimerization of full length or variable domain (VL ) of LC serves to stabilize the native state and prevent the formation of amyloid fibrils. We here analyzed the thermodynamic properties of dimerization and unfolding reactions by nonamyloidogenic VL from REI LC or its monomeric Y96K mutant using sedimentation velocity and circular dichroism. The data indicate that the equilibrium shifts to native dimerization for wild type REI VL by elevating temperature due to the negative enthalpy change for dimer dissociation (-81...
July 24, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28736871/inequitable-health-service-use-in-a-canadian-paediatric-population-a-cross-sectional-study-of-individual-and-contextual-level-factors
#18
C Holtz, J Gilliland, A Thind, P Wilk, M K Campbell
BACKGROUND: Health service use may be influenced by multilevel predisposing, enabling, and need factors but is equitable when driven by need. The study's objectives were as follows: (a) to investigate residential context's effect on child health service use and (b) to examine inequity of child health service use by testing for effect measure modification of need factors. METHODS: The sample of 1,451 children was from a prenatal cohort recruited from London, Ontario, between 2002 and 2004, with follow-up until children were toddler/preschooler-aged...
July 23, 2017: Child: Care, Health and Development
https://www.readbyqxmd.com/read/28736752/inhibitory-activity-of-a-concanavalin-isolated-fraction-from-a-glucosamine-peptides-reaction-system-against-heat-resistant-e-coli
#19
Yuliya Hrynets, Daylin Johana Hincapie Martinez, Maurice Ndagijimana, Mirko Betti
Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were determined. The minimum inhibitory concentration of glycated hydrolysates decreased by 1.2 times compared to the native hydrolysate, with no differences between (+) or (-) TGase treatments...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28736690/decellularization-of-human-internal-mammary-artery-biomechanical-properties-and-histopathological-evaluation
#20
Abdol-Mohammad Kajbafzadeh, Reza Khorramirouz, Seyede Maryam Kameli, Javad Hashemi, Amin Bagheri
This study undertook to create small-diameter vascular grafts and assess their structure and mechanical properties to withstand arterial implantation. Twenty samples of intact human internal mammary arteries (IMAs) were collected and decellularized using detergent-based methods. To evaluate residual cellular and extracellular matrix (ECM) components, histological analysis was performed. Moreover, collagen typing and ECM structure were analyzed by Picrosirius red and Movat's pentachrome staining. Scanning electron microscopy was also applied to assess microarchitecture of both endothelial and adventitial surfaces of native and decellularized arterial samples...
2017: BioResearch Open Access
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