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https://www.readbyqxmd.com/read/29334105/specific-labeling-of-mitochondria-of-chlamydomonas-with-cationic-helicene-fluorophores
#1
Christoph Bauer, Romain Duwald, Geraldine Maria Labrador, Simon Pascal, Pau Moneva Lorente, Johann Bosson, Jérôme Lacour, Jean-David Rochaix
Twelve cationic helicenes and one triangulene were tested for the specific labeling of mitochondria from algal cells. Octyl ester derivative 5 readily penetrates algal cells and gives rise to clear fluorescence patterns when it is used at concentrations in the μM range. Under these conditions, cell structures are well preserved and cell survival is not compromised. Cationic helicene compounds such as 5 provide new useful tools for examining the mitochondrial network and its dynamics including fission and fusion events...
January 15, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29327448/the-saliva-proteome-of-dogs-variations-within-and-between-breeds-and-between-species
#2
Sabah Pasha, Taichi Inui, Iain Chapple, Stephen Harris, Lucy Holcombe, Melissa M Grant
Saliva is a complex multifunctional fluid that bathes the oral cavity to assist in soft and hard tissue maintenance, lubrication, buffering, defence against microbes and initiating digestion of foods. It has been extensively characterised in humans but its protein composition in dogs remains poorly characterised, yet could explain (patho) physiological differences between individuals, breeds and with humans. This pilot discovery study aimed to characterise canine saliva from two breeds, Labrador retrievers and Beagles, and to compare this with human saliva using quantitative mass spectrometry...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29308443/relationship-between-adiponectin-and-apob-in-individuals-with-diabetes-in-the-atlantic-path-cohort
#3
Vanessa DeClercq, Yunsong Cui, Trevor J B Dummer, Cynthia Forbes, Scott A Grandy, Melanie Keats, Louise Parker, Ellen Sweeney, Zhijie Michael Yu, Roger S McLeod
Context: The increasing prevalence of obesity and diabetes greatly influences the risk for cardiovascular (CV) comorbidities and affects the quality of life of many people. However, the relationship among diabetes, obesity, and cardiovascular risk is complex and requires further investigation to understand the biological milieu connecting these conditions. Objective: The aim of the current study was to explore the relationship between biological markers of adipose tissue function (adiponectin) and CV risk (apolipoprotein B) in body mass index (BMI)-matched participants with and without diabetes...
December 1, 2017: Journal of the Endocrine Society
https://www.readbyqxmd.com/read/29260323/occurrences-and-changes-of-disinfection-by-products-in-small-water-supply-systems
#4
Shakhawat Chowdhury
The small water supply systems (WSSs) often report high concentrations of disinfection by-products (DBPs) in drinking water. In this study, occurrences of trihalomethanes (THMs) and haloacetic acids (HAAs) in Newfoundland and Labrador (NL), Canada, were investigated from 441 WSSs for a period of 18 years (1999-2016). The WSSs were divided into groundwater (GWP) and surface water (SWP) systems, which were further classified into eight sub-groups (P1-P8) based on the population served (≤ 100; 101-250; 251-500; 501-1000; 1001-3000; 3001-5000; 5001-10,000; and 10,000+, respectively)...
December 20, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29247849/mitogenomics-supports-an-unexpected-taxonomic-relationship-for-the-extinct-diving-duck-chendytes-lawi-and-definitively-places-the-extinct-labrador-duck
#5
Janet C Buckner, Ryan Ellingson, David A Gold, Terry L Jones, David K Jacobs
Chendytes lawi, an extinct flightless diving anseriform from coastal California, was traditionally classified as a sea duck, tribe Mergini, based on similarities in osteological characters. We recover and analyze mitochondrial genomes of C. lawi and five additional Mergini species, including the extinct Labrador Duck, Camptorhyncus labradorius. Despite its diving morphology, C. lawi is reconstructed as an ancient relictual lineage basal to the dabbling ducks (tribe Anatini), revealing an additional example of convergent evolution of characters related to feeding behavior among ducks...
December 13, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29247721/polymorphisms-in-the-canine-il7r-3-utr-are-associated-with-thymic-output-in-labrador-retriever-dogs-and-influence-post-transcriptional-regulation-by-microrna-185
#6
Angela Holder, Gareth Jones, Francesca Soutter, Donald B Palmer, Richard Aspinall, Brian Catchpole
Interleukin-7 (IL-7) and its receptor (IL-7R) are essential for T cell development in the thymus, and changes in the IL-7/IL-7R pathway have been implicated in age-associated thymic involution which results in a reduction of naïve T cell output. The aim of this study was to investigate the relationship between IL7 and IL7R genetic variation and thymic output in dogs. No single nucleotide polymorphisms (SNPs) were identified in the canine IL7 gene, but a number were present in the canine IL7R gene. Polymorphisms in the IL7R exon 8 and 3'UTR were found to be associated with signal joint T cell receptor excision circle (sj-TREC) values (a biomarker of thymic output) in young and geriatric Labrador retrievers...
December 13, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29241349/distribution-and-clustering-of-cutaneous-t-cell-lymphoma-ctcl-cases-in-canada-during-1992-to-2010
#7
Feras M Ghazawi, Elena Netchiporouk, Elham Rahme, Matthew Tsang, Linda Moreau, Steven Glassman, Nathalie Provost, Martin Gilbert, Sara-Elizabeth Jean, Osama Roshdy, Kevin Pehr, Denis Sasseville, Ivan V Litvinov
BACKGROUND: Clustering of patients with cutaneous T-cell lymphoma (CTCL) was reported in several jurisdictions around the world. This rare cancer is known to affect spouses and in some cases multiple members of the same family. These combined results suggest the existence of external disease triggers/promoters. We recently conducted the first comprehensive analysis of CTCL incidence and mortality in Canada, which revealed case clustering in several regions. OBJECTIVES: To extend our previous analysis on CTCL incidence across Canada and to provide all the collected data on CTCL patient incidence in Canada during the period of 1992 to 2010...
December 1, 2017: Journal of Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/29227139/phenotypic-hip-and-elbow-dysplasia-trends-in-rottweilers-and-labrador-retrievers-in-south-africa-2007-2015-are-we-making-progress
#8
Robert M Kirberger
Canine hip and elbow dysplasia are major orthopaedic problems prevalent the world over, and South Africa is no exception. Hip and elbow dysplasia phenotypic status is certified by a number of different radiographic schemes in the world. South Africa uses the Fédération Cynologique Internationale system to certify hips, and the International Elbow Working Group scheme to certify elbows. One way of reducing these often crippling conditions is by selective breeding using only dogs with no or marginal dysplastic joints...
November 22, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/29203942/inguinal-herniation-of-a-mineralized-paraprostatic-cyst-in-a-dog
#9
Kyle P Vititoe, Federico Vilaplana Grosso, Stephanie Thomovsky, Chee Kin Lim, Hock Gan Heng
A firm mass was noted in the right inguinal subcutaneous region of an 11-year-old intact male Labrador retriever dog presented for right pelvic limb weakness. Pelvic radiographs showed 2 large ovoid structures with circumferential thin eggshell-like mineralization in the right external inguinal region. The structures were confirmed sonographically, and on magnetic resonance imaging as a large folded herniated mineralized paraprostatic cyst through a defect in the right inguinal wall. To the author's knowledge, this is the first published report of an inguinal herniated mineralized paraprostatic cyst...
December 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/29202502/variation-in-the-quantity-of-elastic-fibres-with-degeneration-in-canine-cranial-cruciate-ligaments-from-labrador-retrievers
#10
Kinley D Smith, Kei Hayashi, Dylan N Clements, Peter D Clegg, John F Innes, Eithne J Comerford
Objectives This study aims to quantify numbers of elastic fibres in cranial cruciate ligaments from a dog breed at high risk of cranial cruciate ligament disease. Methods Macroscopically normal cranial cruciate ligaments were harvested from six Labrador retrievers. Sequential histological sections were assessed for extracellular matrix degeneration (haematoxylin and eosin stain) and elastic fibre staining (Miller's stain). Elastic fibres were semi-quantified using previously published scoring systems. Each section was scored twice by two observers...
November 2017: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
https://www.readbyqxmd.com/read/29193392/ancient-chromosomal-rearrangement-associated-with-local-adaptation-of-a-post-glacially-colonized-population-of-atlantic-cod-in-the-northwest-atlantic
#11
Marion Sinclair-Waters, Ian R Bradbury, Corey J Morris, Sigbjørn Lien, Matthew P Kent, Paul Bentzen
Intraspecific diversity is central to the management and conservation of exploited species, yet knowledge of how this diversity is distributed and maintained in the genome of many marine species is lacking. Recent advances in genomic analyses allow for genome-wide surveys of intraspecific diversity and offer new opportunities for exploring genomic patterns of divergence. Here, we analyzed genome-wide polymorphisms to measure genetic differentiation between an offshore migratory and a non-migratory population and to define conservation units of Atlantic Cod (Gadus morhua) in coastal Labrador...
November 28, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29193227/minimally-invasive-lag-screw-fixation-of-sacroiliac-luxation-fracture-using-a-dedicated-novel-instrument-system-apparatus-and-technique-description
#12
Loïc M Déjardin, Albane H Fauron, Laurent P Guiot, Reunan P Guillou
OBJECTIVE: To describe a novel Sacroiliac Luxation Instrument System (SILIS™) and its application in minimally invasive osteosynthesis (MIO) of sacroiliac luxations/fractures (SIL/F). The SILIS was designed to provide stable SIL/F reduction and accurate sacral screw placement while reducing personnel exposure to ionizing radiation during intraoperative fluoroscopy. STUDY DESIGN: Descriptive, proof of concept cadaveric study. METHODS: A right SIL and a left SIL/F were created on a Labrador Retriever that had died of natural causes...
November 29, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29182888/psychometric-assessment-and-precision-remodeling-of-the-iowa-infant-feeding-attitude-scale-to-improve-clinical-use-and-efficacy-among-prenatal-women-in-canada
#13
Nouf M AlKusayer, William K Midodzi, Leigh Anne Newhook, Lorraine Burrage, Nicole Gill, Beth Halfyard, Laurie K Twells
BACKGROUND: The 17-item Iowa Infant Feeding Attitude Scale (IIFAS) has been widely used to assess maternal attitudes toward infant feeding and to predict breastfeeding intention. The IIFAS has been validated among prenatal women located in Newfoundland and Labrador in Canada, although its length may prove challenging to complete in a clinical setting. Research aim: The authors aimed to reduce the number of items from the original 17-item IIFAS scale while maintaining reliability and validity...
November 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/29170428/complex-three-dimensional-self-assembly-in-proxies-for-atmospheric-aerosols
#14
C Pfrang, K Rastogi, E R Cabrera-Martinez, A M Seddon, C Dicko, A Labrador, T S Plivelic, N Cowieson, A M Squires
Aerosols are significant to the Earth's climate, with nearly all atmospheric aerosols containing organic compounds that often contain both hydrophilic and hydrophobic parts. However, the nature of how these compounds are arranged within an aerosol droplet remains unknown. Here we demonstrate that fatty acids in proxies for atmospheric aerosols self-assemble into highly ordered three-dimensional nanostructures that may have implications for environmentally important processes. Acoustically trapped droplets of oleic acid/sodium oleate mixtures in sodium chloride solution are analysed by simultaneous synchrotron small-angle X-ray scattering and Raman spectroscopy in a controlled gas-phase environment...
November 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/29168045/biocompatibility-of-intravitreal-injection-of-human-mesenchymal-stem-cells-in-immunocompetent-rabbits
#15
Sonia Labrador Velandia, Salvatore Di Lauro, Maria Luz Alonso-Alonso, Soraya Tabera Bartolomé, Girish Kumar Srivastava, José Carlos Pastor, Ivan Fernandez-Bueno
PURPOSE: To evaluate the feasibility, safety, and biocompatibility of intravitreal injection of human mesenchymal stem cells (MSCs) in immunocompetent pigmented rabbits. MATERIALS AND METHODS: Thirty-two pigmented rabbits (24 females, 8 males; Chinchilla-New Zealand White) were divided into 8 groups of 4 animals. Commercially prepared human MSCs were injected (0.05 ml) into the post-lens vitreous of the right eyes. Groups 1 and 4 received isotonic medium (Ringer lactate-based), groups 2, 5, 7, and 8 received a low dose of 15 × 10(6) cells/ml...
November 22, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29167464/north-atlantic-variability-and-its-links-to-european-climate-over-the-last-3000-years
#16
Paola Moffa-Sánchez, Ian R Hall
The subpolar North Atlantic is a key location for the Earth's climate system. In the Labrador Sea, intense winter air-sea heat exchange drives the formation of deep waters and the surface circulation of warm waters around the subpolar gyre. This process therefore has the ability to modulate the oceanic northward heat transport. Recent studies reveal decadal variability in the formation of Labrador Sea Water. Yet, crucially, its longer-term history and links with European climate remain limited. Here we present new decadally resolved marine proxy reconstructions, which suggest weakened Labrador Sea Water formation and gyre strength with similar timing to the centennial cold periods recorded in terrestrial climate archives and historical records over the last 3000 years...
November 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/29166281/epidemiological-study-of-canine-mast-cell-tumours-according-to-the-histological-malignancy-grade
#17
Anna Śmiech, B Ślaska, W Łopuszyński, A Jasik, M Szczepanik, P Wilkołek
The aim of the study was to identify significant relationships between the tumour malignancy grade and dogs' age, breed, sex, size, and location of mast cell tumours (MCTs). MCTs accounted for 13.27% of all diagnosed canine skin tumours. The highest incidence was recorded among Boxers, Labrador Retrievers, American Staffordshire Terriers, and Golden Retrievers. Statistical analysis revealed significantly higher probability of occurrence of the grade I mast cell tumour in the French Bulldog in the head, neck, torso, and limb regions, the grade-II mast cell tumour in Boxer, Doberman, Dachshund, shepherds, and setters in the scrotal region, and the grade III mast cell tumour in Shar-Pei in the axilla region...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29163653/axial-pattern-flaps-using-the-deep-circumflex-iliac-artery-superficial-brachial-and-cranial-superficial-epigastric-direct-cutaneous-arteries-in-the-dog
#18
M Shafiuzama, M S Sabarish Babu, A Mohamed, P Sankar, G N Sindhu, S Hemalatha, G Ravi Sundar
Three dogs (Boxer, Labrador Retriever and German Shepherd) between the age of 7-10 years were presented with the history of tumour masses on right caudo-lateral thigh, right cranial forearm and left cranial and caudal thoracic mammary gland region, respectively. Fine needle aspiration cytology of the tumour masses and the sentinel regional lymph nodes were done. Plain radiography was done to rule out distant metastasis. In all the three cases the tumour masses were large in size, firmly adherent to the tissues underneath and sufficient loose skin was not available to close the skin defect following surgery...
2017: Iranian Journal of Veterinary Research
https://www.readbyqxmd.com/read/29155221/motor-axon-guidance-in-drosophila
#19
REVIEW
Aref Arzan Zarin, Juan-Pablo Labrador
The Drosophila motor system starts to assemble during embryonic development. It is composed of 30 muscles per abdominal hemisegment and 36 motor neurons assembling into nerve branches to exit the CNS, navigate within the muscle field and finally establish specific connections with their target muscles. Several families of guidance molecules that play a role controlling this process as well as transcriptional regulators that program the behavior of specific motoneurons have been identified. In this review we summarize the role of both groups of molecules in the motor system as well as their relationship where known...
November 16, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29152242/comparative-assessment-of-canine-origin-lactobacillus-johnsonii-cpn23-and-dairy-origin-lactobacillus-acidophillus-ncdc-15-for-nutrient-digestibility-faecal-fermentative-metabolites-and-selected-gut-health-indices-in-dogs
#20
Sachin Kumar, Ashok K Pattanaik, Shalini Sharma, Reema Gupta, Sunil E Jadhav, Narayan Dutta
The present experiment was undertaken to validate a probiotic of canine origin for its potential use in dogs. A total of fifteen adult female Labrador dogs were allocated to three equal groups and fed a basal diet without probiotic (control) or with probiotic of either canine (Lactobacillus johnsonii CPN23; cPRO) or dairy (L. acidophilus NCDC 15; dPRO) origin for 9 weeks. The digestibility of most macronutrients remained similar among the groups; however, fibre digestibility was improved (P = 0·034) in dogs receiving cPRO...
2017: Journal of Nutritional Science
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