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https://www.readbyqxmd.com/read/28545156/the-boron-transporter-bnac4-bor1-1c-is-critical-for-inflorescence-development-and-fertility-under-boron-limitation-in-brassica-napus
#1
Quan Zhang, Haifei Chen, Mingliang He, Zhuqing Zhao, Hongmei Cai, Guangda Ding, Lei Shi, Fangsen Xu
Boron (B) is an essential micronutrient for plants, but the molecular mechanisms underlying the uptake and distribution of B in allotetraploid rapeseed (Brassica napus) are unclear. Here, we identified a B transporter of rapeseed, BnaC4.BOR1;1c, which expressed in shoot nodes and involved in distributing B to the reproductive organs. Transgenic Arabidopsis plants containing a BnaC4.BOR1;1c promoter-driven GUS reporter gene showed strong GUS activity in roots, nodal regions of the shoots and immature floral buds...
May 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28544671/a-common-thermal-niche-among-geographically-diverse-populations-of-the-widely-distributed-tree-species-eucalyptus-tereticornis-no-evidence-for-adaptation-to-climate-of-origin
#2
John E Drake, Angelica Vårhammar, Dushan Kumarathunge, Belinda E Medlyn, Sebastian Pfautsch, Peter B Reich, David T Tissue, Oula Ghannoum, Mark G Tjoelker
Impacts of climate warming depend on the degree to which plants are constrained by adaptation to their climate-of-origin or exhibit broad climatic suitability. We grew cool-origin, central and warm-origin provenances of Eucalyptus tereticornis in an array of common temperature environments from 18 to 35.5°C to determine if this widely distributed tree species consists of geographically contrasting provenances with differentiated and narrow thermal niches, or if provenances share a common thermal niche. The temperature responses of photosynthesis, respiration, and growth were equivalent across the three provenances, reflecting a common thermal niche despite a 2,200 km geographic distance and 13°C difference in mean annual temperature at seed origin...
May 23, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28543910/wheatgrass-derived-polysaccharide-has-antiinflammatory-anti-oxidative-and-anti-apoptotic-effects-on-lps-induced-hepatic-injury-in-mice
#3
Sarmila Nepali, Hyeon-Hui Ki, Ji-Hyun Lee, Hoon-Yeon Lee, Dae-Ki Kim, Young-Mi Lee
Hepatic injury occurs frequently during sepsis, and polysaccharides isolated from plants have been reported to have antiinflammatory and antioxidant effects in various models. However, the effect of wheatgrass-derived polysaccharide (WGP) has not been previously studied. In the present study, we investigated the effect of WGP on lipopolysaccharide (LPS)-induced hepatic injury in mice. Mice were pre-treated with WGP (100 or 200 mg/kg daily for 2 days) and then challenged with LPS (1 mg/kg, intraperitoneal), and sacrificed after 12 h...
May 24, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28542547/determination-of-diagnostic-standards-on-saturated-soil-extracts-for-cut-roses-grown-in-greenhouses
#4
John Jairo Franco-Hermida, María Fernanda Quintero, Raúl Iskander Cabrera, José Miguel Guzman
This work comprises the theoretical determination and validation of diagnostic standards for the analysis of saturated soil extracts for cut rose flower crops (Rosa spp.) growing in the Bogota Plateau, Colombia. The data included 684 plant tissue analyses and 684 corresponding analyses of saturated soil extracts, all collected between January 2009 and June 2013. The tissue and soil samples were selected from 13 rose farms, and from cultivars grafted on the 'Natal Briar' rootstock. These concurrent samples of soil and plant tissues represented 251 production units (locations) of approximately 10,000 m2 distributed across the study area...
2017: PloS One
https://www.readbyqxmd.com/read/28542545/a-first-insight-into-the-involvement-of-phytohormones-pathways-in-coffee-resistance-and-susceptibility-to-colletotrichum-kahawae
#5
Inês Diniz, Andreia Figueiredo, Andreia Loureiro, Dora Batista, Helena Azinheira, Vítor Várzea, Ana Paula Pereira, Elijah Gichuru, Pilar Moncada, Leonor Guerra-Guimarães, Helena Oliveira, Maria do Céu Silva
Understanding the molecular mechanisms underlying coffee-pathogen interactions are of key importance to aid disease resistance breeding efforts. In this work the expression of genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) pathways were studied in hypocotyls of two coffee varieties challenged with the hemibiotrophic fungus Colletotrichum kahawae, the causal agent of Coffee Berry Disease. Based on a cytological analysis, key time-points of the infection process were selected and qPCR was used to evaluate the expression of phytohormones biosynthesis, reception and responsive-related genes...
2017: PloS One
https://www.readbyqxmd.com/read/28542282/expression-patterns-of-members-of-the-ethylene-signaling-related-gene-families-in-response-to-dehydration-stresses-in-cassava
#6
Meng Yun Ren, Ren Jun Feng, Hou Rui Shi, Li Fang Lu, Tian Yan Yun, Ming Peng, Xiao Guan, Heng Zhang, Jing Yi Wang, Xi Yan Zhang, Cheng Liang Li, Yan Jun Chen, Peng He, Yin Dong Zhang, Jiang Hui Xie
Drought is the one of the most important environment stresses that restricts crop yield worldwide. Cassava (Manihot esculenta Crantz) is an important food and energy crop that has many desirable traits such as drought, heat and low nutrients tolerance. However, the mechanisms underlying drought tolerance in cassava are unclear. Ethylene signaling pathway, from the upstream receptors to the downstream transcription factors, plays important roles in environmental stress responses during plant growth and development...
2017: PloS One
https://www.readbyqxmd.com/read/28541416/serial-block-face-sem-visualization-of-unusual-plant-nuclear-tubular-extensions-in-a-carnivorous-plant-utricularia-lentibulariaceae
#7
Bartosz J Plachno, Piotr Swiatek, Richard W Jobson, Karol Malota, Wojciech Brutkowski
Background and Aims: In Utricularia nelumbifolia , the nuclei of placental nutritive tissue possess unusually shaped projections not known to occur in any other flowering plant. The main aim of the study was to document the morphology and ultrastructure of these unusual nuclei. In addition, the literature was searched to find examples of nuclear tubular projections in other plant groups, and the nuclei of closely related species of Utricularia (i.e. sects Iperua , Orchidioides , Foliosa and Utricularia ) were examined...
May 24, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28541415/transcriptome-wide-identification-and-expression-analysis-of-divaricata-and-radialis-like-genes-of-the-mediterranean-orchid-orchis-italica
#8
Maria Carmen Valoroso, Sofia De Paolo, Giovanni Iazzetti, Serena Aceto
Bilateral symmetry of flowers is a relevant novelty that has occurred many times throughout the evolution of flowering plants. In Antirrhinum majus, establishment of flower dorso-ventral asymmetry is mainly due to interaction of TCP (CYC and DICH) and MYB (DIV, RAD and DRIF) transcription factors. In the present study, we characterized 8 DIV-, 4 RAD- and 2 DRIF-like genes from the transcriptome of Orchis italica, an orchid species with bilaterally symmetric and resupinate flowers. We found a similar number of DIV- and RAD-like genes within the genomes of Phalaenopsis equestris and Dendrobium catenatum orchids...
May 25, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28540497/large-scale-transcriptome-analysis-reveals-arabidopsis-metabolic-pathways-are-frequently-influenced-by-different-pathogens
#9
Zhenhong Jiang, Fei He, Ziding Zhang
Through large-scale transcriptional data analyses, we highlighted the importance of plant metabolism in plant immunity and identified 26 metabolic pathways that were frequently influenced by the infection of 14 different pathogens. Reprogramming of plant metabolism is a common phenomenon in plant defense responses. Currently, a large number of transcriptional profiles of infected tissues in Arabidopsis (Arabidopsis thaliana) have been deposited in public databases, which provides a great opportunity to understand the expression patterns of metabolic pathways during plant defense responses at the systems level...
May 24, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28539971/a-fast-efficient-chromatin-immunoprecipitation-method-for-studying-protein-dna-binding-in-arabidopsis-mesophyll-protoplasts
#10
Jeong Hwan Lee, Suhyun Jin, Sun Young Kim, Wanhui Kim, Ji Hoon Ahn
BACKGROUND: Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an indispensable tool used to investigate the binding of DNA-binding proteins (e.g., transcription factors) to their target sequences in vivo. ChIP assays require specific antibodies that recognize endogenous target transcription factors; however, in most cases, such specific antibodies are unavailable...
2017: Plant Methods
https://www.readbyqxmd.com/read/28536883/identification-of-genes-related-to-skin-development-in-potato
#11
Vijaya K R Vulavala, Edna Fogelman, Lior Rozental, Adi Faigenboim, Zachariah Tanami, Oded Shoseyov, Idit Ginzberg
Newly identified genes that are preferentially expressed in potato skin include genes that are associated with the secondary cell wall and stress-related activities and contribute to the skin's protective function. Microarrays were used to compare the skin and tuber-flesh transcriptomes of potato, to identify genes that contribute to the unique characteristics of the skin as a protective tissue. Functional gene analysis indicated that genes involved in developmental processes such as cell division, cell differentiation, morphogenesis and secondary cell wall formation (lignification and suberization), and stress-related activities, are more highly expressed in the skin than in the tuber flesh...
May 23, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28536583/sediment-resuspension-and-deposition-on-seagrass-leaves-impedes-internal-plant-aeration-and-promotes-phytotoxic-h2s-intrusion
#12
Kasper E Brodersen, Kathrine J Hammer, Verena Schrameyer, Anja Floytrup, Michael A Rasheed, Peter J Ralph, Michael Kühl, Ole Pedersen
HIGHLIGHTS: Sedimentation of fine sediment particles onto seagrass leaves severely hampers the plants' performance in both light and darkness, due to inadequate internal plant aeration and intrusion of phytotoxic H2S. Anthropogenic activities leading to sediment re-suspension can have adverse effects on adjacent seagrass meadows, owing to reduced light availability and the settling of suspended particles onto seagrass leaves potentially impeding gas exchange with the surrounding water. We used microsensors to determine O2 fluxes and diffusive boundary layer (DBL) thickness on leaves of the seagrass Zostera muelleri with and without fine sediment particles, and combined these laboratory measurements with in situ microsensor measurements of tissue O2 and H2S concentrations...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28536283/signal-integration-in-quorum-sensing-enables-cross-species-induction-of-virulence-in-pectobacterium-wasabiae
#13
Rita S Valente, Pol Nadal-Jimenez, André F P Carvalho, Filipe J D Vieira, Karina B Xavier
Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks-the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway-control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28536145/monoterpenes-support-systemic-acquired-resistance-within-and-between-plants
#14
Marlies Riedlmeier, Andrea Ghirardo, Marion Wenig, Claudia Knappe, Kerstin Koch, Elisabeth Georgii, Sanjukta Dey, Jane E Parker, Joerg-Peter Schnitzler, Corina Vlot
This study investigates the role of volatile organic compounds in systemic acquired resistance (SAR), a salicylic acid (SA)-associated, broad-spectrum immune response in systemic, healthy tissues of locally infected plants. Gas chromatography coupled to mass spectrometry analyses of SAR-related emissions of wild-type and non-SAR-signal-producing mutant plants associated SAR with monoterpene emissions. Headspace exposure of Arabidopsis thaliana to a mixture of the bicyclic monoterpenes α-pinene and β-pinene induced defense, accumulation of reactive oxygen species, and expression of SA- and SAR-related genes, including the SAR regulatory AZELAIC ACID INDUCED1 (AZI1) gene and three of its paralogs...
May 23, 2017: Plant Cell
https://www.readbyqxmd.com/read/28536103/repression-of-cystathionine-%C3%AE-synthase-in-seeds-recruits-the-s-methylmethionine-cycle
#15
Hagai Cohen, Yael Hacham, Irina Panizel, Ilana Rogachev, Asaph Aharoni, Rachel Amir
S-methylmethionine (SMM) was previously suggested to participate in the metabolism of methionine in seeds. To further reveal its roles, we had previously produced transgenic Arabidopsis RNAi seeds with lower transcript expression of CYSTATHIONINE γ-SYNTHASE (AtCGS), methionine's main regulatory enzyme. Unexpectedly, these seeds accumulated significantly higher levels of methionine compared to control seeds through an as yet unknown mechanism. Here, transcript and metabolic analyses coupled with isotope-labeled [13C]SMM and [13C]methionine feeding experiments enabled us to reveal that SMM that were synthesized in rosette leaves of RNAi plants significantly contributed to the accumulation of methionine in their seeds at late stages of development...
May 23, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28534650/functions-of-iqd-proteins-as-hubs-in-cellular-calcium-and-auxin-signaling-a-toolbox-for-shape-formation-and-tissue-specification-in-plants
#16
Katharina Bürstenbinder, Dipannita Mitra, Jakob Quegwer
Ca(2+) ions play pivotal roles as second messengers in intracellular signal transduction, and coordinate many biological processes. Changes in intracellular Ca(2+) levels are perceived by Ca(2+) sensors such as CaM/CML proteins, which transduce Ca(2+) signals into cellular responses by regulation of diverse target proteins. Insights into molecular functions of CaM targets are thus essential to understand the molecular and cellular basis of Ca(2+) signaling. During the last decade, IQD proteins emerged as the largest class of CaM targets in plants with mostly unknown functions...
May 23, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28534439/hypolipidemic-and-hepatoprotective-activities-of-rosemary-and-thyme-in-gentamicin-treated-rats
#17
A M Hegazy, A S Abdel-Azeem, H M Zeidan, K S Ibrahim, Em El Sayed
Rosemary ( Rosmarinus officinalis; RM) and thyme ( Thymus vulgaris; TV) are used as remedies. We designed this work to investigate the preventive effect of aqueous extracts of RM or TV on the gentamicin (GM)-induced hepatotoxicity and abnormalities of lipid profile in rats. Both plant extracts exhibited an in vitro antioxidant activity as determined by the 1,1-diphenyl-2-picrylhydrazyl assay. Radical scavenging activity for TV extract was 48.7% and for RM extract was 25.6%. Polyphenolic determination by high-performance liquid chromatography for both extracts revealed that catechin, coumarin, cinnamic acid and rutin were detected in both extracts...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28534307/acanthus-ilicifolius-l-a-promising-candidate-for-phytostabilization-of-zinc
#18
A M Shackira, Jos T Puthur, E Nabeesa Salim
The potential of a halophyte species-Acanthus ilicifolius L.-to phytostabilize zinc (Zn) grown under hydroponics culture conditions was critically evaluated in this study. The propagules after treating with ZnSO4 (4 mM) were analysed for the bioaccumulation pattern, translocation rate of Zn to the shoot, effects of Zn accumulation on organic solutes and the antioxidant defence system. It was found that most of the Zn absorbed by the plant was retained in the root (47%) and only a small portion was transported to stem (12%) and leaves (11%)...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28534166/in-silico-identification-and-validation-of-potential-micrornas-in-kinnow-mandarin-citrus-reticulata-blanco
#19
Prashant Mohanpuria, Naveen Duhan, Navraj Kaur Sarao, Manvir Kaur, Mandip Kaur
MicroRNAs (miRNAs) are a large family of 19-25 nucleotides, regulatory, non-coding RNA molecules that control gene expression by cleaving or inhibiting the translation of target gene transcripts in animals and plants. Despite the important functions of miRNAs related to regulation of plant growth and development processes, metabolism, and abiotic and biotic stresses, little is known about the disease-related miRNA. Here, we present a new pipeline for miRNA analysis using expressed sequence tags (ESTs)-based bioinformatics approach in Kinnow mandarin, a commercially important citrus fruit crop...
May 22, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/28533813/rapid-identification-of-growth-years-and-profiling-of-bioactive-ingredients-in-astragalus-membranaceus-var-mongholicus-huangqi-roots-from-hunyuan-shanxi
#20
Hua-Sheng Peng, Jun Wang, He-Ting Zhang, Hai-Yan Duan, Xiao-Mei Xie, Ling Zhang, Ming-En Cheng, Dai-Yin Peng
BACKGROUND: The content of medicinal bioactive constituents in huangqi is affected by plant age. In this study, we devised a quick and convenient method for determining the age of huangqi, which was cultivated in Hunyuan County (Shanxi Province). METHODS: 1, 2, 3, 4, 5, 8, 10 growth years huangqi had 38 samples, all samples were collected separately. The growth rings in these samples were observed after making paraffin section and freehand-section. The relationship between growth rings and its growth years was analyzed by SPSS 19...
2017: Chinese Medicine
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