keyword
MENU ▼
Read by QxMD icon Read
search

"Cardiac tissue engineering"

keyword
https://www.readbyqxmd.com/read/29107590/human-pluripotent-stem-cell-derived-cardiac-tissue-like-constructs-for-repairing-the-infarcted-myocardium
#1
Junjun Li, Itsunari Minami, Motoko Shiozaki, Leqian Yu, Shin Yajima, Shigeru Miyagawa, Yuji Shiba, Nobuhiro Morone, Satsuki Fukushima, Momoko Yoshioka, Sisi Li, Jing Qiao, Xin Li, Lin Wang, Hidetoshi Kotera, Norio Nakatsuji, Yoshiki Sawa, Yong Chen, Li Liu
High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We show that multilayered and elongated CMs could be organized at high density along aligned nanofibers in a simple one-step seeding process, resulting in upregulated cardiac biomarkers and enhanced cardiac functions...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29053443/enhancement-of-intercellular-electrical-synchronization-by-conductive-materials-in-cardiac-tissue-engineering
#2
Yu Wu, Liang Guo
OBJECTIVE: cardiac tissue regeneration for the treatment of cardiovascular diseases has been of great research interest. Under the hypothesis that electrical synchronization of cardiac cells can be aided by conductive materials, electrically conductive scaffolds have been frequently used to improve cardiac tissue regeneration. However, theoretical analysis is presently absent in examining the underlying mechanism and rationally guiding the design of these conductive scaffolds. METHODS: here, equivalent-circuit models are proposed, in which two adjacent groups of cardiomyocytes are grown either on a bulk conductive substrate or around conductive nanostructures...
October 15, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/29052369/protein-polysaccharide-based-scaffolds-mimicking-native-extracellular-matrix-for-cardiac-tissue-engineering-applications
#3
Elisabetta Rosellini, Yu Shrike Zhang, Bianca Migliori, Niccoletta Barbani, Luigi Lazzeri, Su Ryon Shin, Mehmet Remzi Dokmeci, Maria Grazia Cascone
Tissue engineering has emerged as a viable approach to treat disease or repair damage in tissues and organs. One of the key elements for the success of tissue engineering is the use of a scaffold serving as artificial extracellular matrix (ECM). The ECM hosts the cells and improves their survival, proliferation, and differentiation, enabling the formation of new tissue. Here, we propose the development of a class of protein/polysaccharide-based porous scaffolds for use as ECM substitutes in cardiac tissue engineering...
October 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28951744/igf1-and-nrg1-enhance-proliferation-metabolic-maturity-and-the-force-frequency-response-in-hesc-derived-engineered-cardiac-tissues
#4
Cassady E Rupert, Kareen L K Coulombe
Insulin-like growth factor 1 (IGF1) and neuregulin-1β (NRG1) play important roles during cardiac development both individually and synergistically. In this study, we analyze how 3D cardiac tissue engineered from human embryonic stem cell- (hESC-) derived cardiomyocytes and 2D-plated hESC-cardiomyocytes respond to developmentally relevant growth factors both to stimulate maturity and to characterize the therapeutic potential of IGF1 and NRG1. When administered to engineered cardiac tissues, a significant decrease in active force production of ~65% was measured in all treatment groups, likely due to changes in cellular physiology...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28923324/regenerating-heart-using-a-novel-compound-and-human-wharton-jelly-mesenchymal-stem-cells
#5
Shahram Rabbani, Masoud Soleimani, Mohammad Imani, Mohammad Sahebjam, Ali Ghiaseddin, Seyed Mahdi Nassiri, Jalil Majd Ardakani, Maryam Tajik Rostami, Arash Jalali, Bahmanshir Mousanassab, Mahsa Kheradmandi, Seyed Hossein Ahmadi Tafti
BACKGROUND: Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disputing. AIM OF THE STUDY: In this study a combination of human Wharton jelly mesenchymal stem cells (HWJMSCs) with a novel compound consisting polyethylene glycol (PEG), hyaluronic acid and chitosan is presented to heart regeneration...
April 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/28895403/injectable-carbon-nanotube-functionalized-reverse-thermal-gel-promotes-cardiomyocytes-survival-and-maturation
#6
Brisa Peña, Susanna Bosi, Brian A Aguado, Daniele Borin, Nikki L Farnsworth, Evgenia Dobrinskikh, Teisha J Rowland, Valentina Martinelli, Mark Jeong, Matthew R G Taylor, Carlin S Long, Robin Shandas, Orfeo Sbaizero, Maurizio Prato, Kristi S Anseth, Daewon Park, Luisa Mestroni
The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used to improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a solution at room temperature to a three-dimensional (3D) gel-based matrix shortly after reaching body temperature...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28875579/a-cardiac-patch-from-aligned-microvessel-and-cardiomyocyte-patches
#7
Jeremy A Schaefer, Pilar A Guzman, Sonja B Riemenschneider, Timothy J Kamp, Robert T Tranquillo
Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack pre-formed microvascularization and are therefore limited in thickness and force production. In the present study, we sought to assess whether a bi-layer patch composed of a layer made from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and a microvessel layer composed of self-assembled human blood outgrowth endothelial cells (BOECs) and pericytes (PCs) was capable of engrafting on the epicardial surface of a nude rat infarct model and becoming perfused by the host four weeks after acute implantation...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28875152/artificial-cardiac-muscle-with-or-without-the-use-of-scaffolds
#8
REVIEW
Yifei Li, Donghui Zhang
During the past several decades, major advances and improvements now promote better treatment options for cardiovascular diseases. However, these diseases still remain the single leading cause of death worldwide. The rapid development of cardiac tissue engineering has provided the opportunity to potentially restore the contractile function and retain the pumping feature of injured hearts. This conception of cardiac tissue engineering can enable researchers to produce autologous and functional biomaterials which represents a promising technique to benefit patients with cardiovascular diseases...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28857113/myofibrils-in-cardiomyocytes-tend-to-assemble-along-the-maximal-principle-stress-directions
#9
Hongyan Yuan, Bahador Marzban, Kevin Kit Parker
The mechanisms underlying the spatial organization of self-assembled myofibrils in cardiac tissues remain incompletely understood. By modeling cells as elastic solids under active cytoskeletal contraction, we found a good correlation between the predicted maximal principal stress directions and the in vitro myofibril orientations in individual cardiomyocytes. This implies that actomyosin fibers tend to assemble along the maximal tensile stress (MTS) directions. By considering the dynamics of focal adhesion and myofibril formation in the model, we showed that different patterns of myofibril organizations in mature versus immature cardiomyocytes can be explained as the consequence of the different levels of force-dependent remodeling of focal adhesions...
December 1, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28832034/surface-modified-polymers-for-cardiac-tissue-engineering
#10
REVIEW
Ambigapathi Moorthi, Yu-Chang Tyan, Tze-Wen Chung
Cardiovascular disease (CVD), leading to myocardial infarction and heart failure, is one of the major causes of death worldwide. The physiological system cannot significantly regenerate the capabilities of a damaged heart. The current treatment involves pharmacological and surgical interventions; however, less invasive and more cost-effective approaches are sought. Such new approaches are developed to induce tissue regeneration following injury. Hence, regenerative medicine plays a key role in treating CVD...
September 26, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28770811/fabrication-of-arbitrary-3d-components-in-cardiac-surgery-from-macro-micro-to-nanoscale
#11
Ranjith Kumar Kankala, Kai Zhu, Jun Li, Chun-Sheng Wang, Shi-Bin Wang, Ai-Zheng Chen
Fabrication of tissue-/organ-like structures at arbitrary geometries by mimicking the properties of the complex material offers enormous interest to the research and clinical applicability in cardiovascular diseases. Patient-specific, durable, and realistic three-dimensional (3D) cardiac models for anatomic consideration have been developed for education, pro-surgery planning, and intra-surgery guidance. In cardiac tissue engineering (TE), 3D printing technology is the most convenient and efficient microfabrication method to create biomimetic cardiovascular tissue for the potential in vivo implantation...
August 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28720761/parylene-c-topographic-micropattern-as-a-template-for-patterning-pdms-and-polyacrylamide-hydrogel
#12
Ilaria Sanzari, Mauro Callisti, Antonio De Grazia, Daniel J Evans, Tomas Polcar, Themistoklis Prodromakis
Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28699224/melt-electrospinning-writing-of-poly-hydroxymethylglycolide-co-%C3%AE%C2%B5-caprolactone-based-scaffolds-for-cardiac-tissue-engineering
#13
Miguel Castilho, Dries Feyen, María Flandes-Iparraguirre, Gernot Hochleitner, Jürgen Groll, Pieter A F Doevendans, Tina Vermonden, Keita Ito, Joost P G Sluijter, Jos Malda
Current limitations in cardiac tissue engineering revolve around the inability to fully recapitulate the structural organization and mechanical environment of native cardiac tissue. This study aims at developing organized ultrafine fiber scaffolds with improved biocompatibility and architecture in comparison to the traditional fiber scaffolds obtained by solution electrospinning. This is achieved by combining the additive manufacturing of a hydroxyl-functionalized polyester, (poly(hydroxymethylglycolide-co-ε-caprolactone) (pHMGCL), with melt electrospinning writing (MEW)...
July 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28683653/optimization-of-human-myocardium-decellularization-method-for-the-construction-of-implantable-patches
#14
Franca Di Meglio, Daria Nurzynska, Veronica Romano, Rita Miraglia, Immacolata Belviso, Anna Maria Sacco, Valeria Barbato, Mariagrazia Di Gennaro, Giuseppina Granato, Ciro Maiello, Stefania Montagnani, Clotilde Castaldo
Cardiac tissue engineering by means of synthetic or natural scaffolds combined with stem/progenitor cells is emerging as the response to the unsatisfactory outcome of approaches based solely on the injection of cells. Parenchymal and supporting cells are surrounded, in vivo, by a specialized and tissue-specific microenvironment, consisting mainly of extracellular matrix (ECM) and soluble factors incorporated in the ECM. Since the naturally occurring ECM is the ideal platform for ensuring cell engraftment, survival, proliferation, and differentiation, the acellular native ECM appears by far the most promising and appealing substrate among all biomaterials tested so far...
September 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28664892/potential-of-propagation-based-synchrotron-x-ray-phase-contrast-computed-tomography-for-cardiac-tissue-engineering
#15
Mohammad Izadifar, Paul Babyn, Dean Chapman, Michael E Kelly, Xiongbiao Chen
Hydrogel-based cardiac tissue engineering offers great promise for myocardial infarction repair. The ability to visualize engineered systems in vivo in animal models is desired to monitor the performance of cardiac constructs. However, due to the low density and weak X-ray attenuation of hydrogels, conventional radiography and micro-computed tomography are unable to visualize the hydrogel cardiac constructs upon their implantation, thus limiting their use in animal systems. This paper presents a study on the optimization of synchrotron X-ray propagation-based phase-contrast imaging computed tomography (PCI-CT) for three-dimensional (3D) visualization and assessment of the hydrogel cardiac patches...
July 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28663141/electrospun-conductive-nanofibrous-scaffolds-for-engineering-cardiac-tissue-and-3d-bioactuators
#16
Ling Wang, Yaobin Wu, Tianli Hu, Baolin Guo, Peter X Ma
Mimicking the nanofibrous structure similar to extracellular matrix and conductivity for electrical propagation of native myocardium would be highly beneficial for cardiac tissue engineering and cardiomyocytes-based bioactuators. Herein, we developed conductive nanofibrous sheets with electrical conductivity and nanofibrous structure composed of poly(l-lactic acid) (PLA) blending with polyaniline (PANI) for cardiac tissue engineering and cardiomyocytes-based 3D bioactuators. Incorporating of varying contents of PANI from 0wt% to 3wt% into the PLA polymer, the electrospun nanofibrous sheets showed enhanced conductivity while maintaining the same fiber diameter...
September 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28638487/current-strategies-and-challenges-for-purification-of-cardiomyocytes-derived-from-human-pluripotent-stem-cells
#17
REVIEW
Kiwon Ban, Seongho Bae, Young-Sup Yoon
Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) are considered a most promising option for cell-based cardiac repair. Hence, various protocols have been developed for differentiating hPSCs into CMs. Despite remarkable improvement in the generation of hPSC-CMs, without purification, these protocols can only generate mixed cell populations including undifferentiated hPSCs or non-CMs, which may elicit adverse outcomes. Therefore, one of the major challenges for clinical use of hPSC-CMs is the development of efficient isolation techniques that allow enrichment of hPSC-CMs...
2017: Theranostics
https://www.readbyqxmd.com/read/28634611/micro-and-nano-patterned-conductive-graphene-peg-hybrid-scaffolds-for-cardiac-tissue-engineering
#18
Alec S T Smith, Hyok Yoo, Hyunjung Yi, Eun Hyun Ahn, Justin H Lee, Guozheng Shao, Ekaterina Nagornyak, Michael A Laflamme, Charles E Murry, Deok-Ho Kim
A lack of electrical conductivity and structural organization in currently available biomaterial scaffolds limits their utility for generating physiologically representative models of functional cardiac tissue. Here we report on the development of scalable, graphene-functionalized topographies with anisotropic electrical conductivity for engineering the structural and functional phenotypes of macroscopic cardiac tissue constructs. Guided by anisotropic electroconductive and topographic cues, the tissue constructs displayed structural property enhancement in myofibrils and sarcomeres, and exhibited significant increases in the expression of cell-cell coupling and calcium handling proteins, as well as in action potential duration and peak calcium release...
June 29, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28629100/biomaterials-and-cells-for-cardiac-tissue-engineering-current-choices
#19
REVIEW
Rusha Chaudhuri, Madhumitha Ramachandran, Pearl Moharil, Megha Harumalani, Amit K Jaiswal
The major purpose of cardiac tissue engineering is to engineer cells on scaffolds and use it as a substitute to infarcted cardiac cells. With an ever-increasing risk of cardiac diseases there is an increasing need to have a stable and sustainable approach to cure such ailments. This review provides a comprehensive update on the cell sources and biomaterials essential for cardiac tissue engineering, ensuring their biocompatibility under a variety of conditions. Cells can be obtained from allogenic or autologous sources...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28590127/interwoven-aligned-conductive-nanofiber-yarn-hydrogel-composite-scaffolds-for-engineered-3d-cardiac-anisotropy
#20
Yaobin Wu, Ling Wang, Baolin Guo, Peter X Ma
Mimicking the anisotropic cardiac structure and guiding 3D cellular orientation play a critical role in designing scaffolds for cardiac tissue regeneration. Significant advances have been achieved to control cellular alignment and elongation, but it remains an ongoing challenge for engineering 3D cardiac anisotropy using these approaches. Here, we present a 3D hybrid scaffold based on aligned conductive nanofiber yarns network (NFYs-NET, composition: polycaprolactone, silk fibroin, and carbon nanotubes) within a hydrogel shell for mimicking the native cardiac tissue structure, and further demonstrate their great potential for engineering 3D cardiac anisotropy for cardiac tissue engineering...
June 27, 2017: ACS Nano
keyword
keyword
26798
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"