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https://www.readbyqxmd.com/read/27911392/optogenetic-random-mutagenesis-using-histone-minisog-in-c-elegans
#1
Kentaro Noma, Yishi Jin
Forward genetic screening in model organisms is the workhorse to discover functionally important genes and pathways in many biological processes. In most mutagenesis-based screens, researchers have relied on the use of toxic chemicals, carcinogens, or irradiation, which requires designated equipment, safety setup, and/or disposal of hazardous materials. We have developed a simple approach to induce heritable mutations in C. elegans using germline-expressed histone-miniSOG, a light-inducible potent generator of reactive oxygen species...
November 14, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910937/biohydrogen-production-from-enzymatic-hydrolysis-of-food-waste-in-batch-and-continuous-systems
#2
Wei Han, Yingting Yan, Yiwen Shi, Jingjing Gu, Junhong Tang, Hongting Zhao
In this study, the feasibility of biohydrogen production from enzymatic hydrolysis of food waste was investigated. Food waste (solid-to-liquid ratio of 10%, w/v) was first hydrolyzed by commercial glucoamylase to release glucose (24.35 g/L) in the food waste hydrolysate. Then, the obtained food waste hydrolysate was used as substrate for biohydrogen production in the batch and continuous (continuous stirred tank reactor, CSTR) systems. It was observed that the maximum cumulative hydrogen production of 5850 mL was achieved with a yield of 245...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910761/investigation-of-requisites-for-the-optimal-mycelial-growth-of-the-lingzhi-or-reishi-medicinal-mushroom-ganoderma-lucidum-agaricomycetes-on-oil-palm-biomass-in-malaysia
#3
Surya Sudheer, Asgar Ali, Sivakumar Manickam
Rigorous research has been carried out regarding the cultivation of Ganoderma lucidum using different agricultural residues. Nevertheless, large-scale cultivation and the separation of active compounds of G. lucidum are still challenges for local farmers. The objective of this study was to evaluate the use of oil palm waste fibers such as empty fruit bunch fibers and mesocarp fibers as effective substrates for the growth of G. lucidum mycelia to study the possibility of solid-state cultivation and to determine the optimum conditions necessary for the growth of mycelia of this mushroom on these waste fibers...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910262/enterobacter-sp-lu1-as-a-novel-succinic-acid-producer-co-utilization-of-glycerol-and-lactose
#4
Marcin Podleśny, Piotr Jarocki, Jakub Wyrostek, Tomasz Czernecki, Jagoda Kucharska, Anna Nowak, Zdzisław Targoński
Succinic acid is an important C4-building chemical platform for many applications. A novel succinic acid-producing bacterial strain was isolated from goat rumen. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the genus Enterobacter. This is the first report of a wild bacterial strain from the genus Enterobacter that is capable of efficient succinic acid production. Co-fermentation of glycerol and lactose significantly improved glycerol utilization under anaerobic conditions, debottlenecking the utilization pathway of this valuable biodiesel waste product...
December 1, 2016: Microbial Biotechnology
https://www.readbyqxmd.com/read/27909923/quality-improvement-of-acidic-soils-by-biochar-derived-from-renewable-materials
#5
Deok Hyun Moon, Inseong Hwang, Yoon-Young Chang, Agamemnon Koutsospyros, Kyung Hoon Cheong, Won Hyun Ji, Jeong-Hun Park
Biochar derived from waste plant materials and agricultural residues was used to improve the quality of an acidic soil. The acidic soil was treated for 1 month with both soy bean stover-derived biochar and oak-derived biochar in the range of 1 to 5 wt% for pH improvement and exchangeable cation enhancement. Following 1 month of treatment, the soil pH was monitored and exchangeable cations were measured. Moreover, a maize growth experiment was performed for 14 days with selected treated soil samples to confirm the effectiveness of the treatment...
December 1, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27909410/mechanosensitive-molecular-networks-involved-in-transducing-resistance-exercise-signals-into-muscle-protein-accretion
#6
REVIEW
Emil Rindom, Kristian Vissing
Loss of skeletal muscle myofibrillar protein with disease and/or inactivity can severely deteriorate muscle strength and function. Strategies to counteract wasting of muscle myofibrillar protein are therefore desirable and invite for considerations on the potential superiority of specific modes of resistance exercise and/or the adequacy of low load resistance exercise regimens as well as underlying mechanisms. In this regard, delineation of the potentially mechanosensitive molecular mechanisms underlying muscle protein synthesis (MPS), may contribute to an understanding on how differentiated resistance exercise can transduce a mechanical signal into stimulation of muscle accretion...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27909352/solid-phase-extraction-of-tritiated-contaminants-from-tritium-containing-waste-oils
#7
Andrzej Olejniczak, Jacek Fall, Katarzyna Olejniczak, Marina V Gustova, Alexandr G Shostenko
A solid phase extraction method for removing polar tritiated contaminants from tritium-containing waste oils has been developed. The composition of the degradation products present in the waste oil was determined. The results indicated that upon exposure to tritium gas, fragment methyl ketones, carboxylic acids, and lactones were the main polar products of the mineral-based oil oxidation. The nonpolar fragmentation products included n-alkanes, monomethylalkanes, and acyclic isoprenoids and were analogous to those formed during [Formula: see text]-irradiation of the oil...
2016: Journal of Radioanalytical and Nuclear Chemistry
https://www.readbyqxmd.com/read/27909212/black-carbon-emission-reduction-strategies-in-healthcare-industry-for-effective-global-climate-change-management
#8
Emilia Mmbando Raila, David O Anderson
Climate change remains one of the biggest threats to life on earth to date with black carbon (BC) emissions or smoke being the strongest cause after carbon dioxide (CO2). Surprisingly, scientific evidence about black carbon emissions reduction in healthcare settings is sparse. This paper presents new research findings on the reduction of black carbon emissions from an observational study conducted at the UN Peacekeeping Operations (MINUSTAH) in Haiti in 2014. Researchers observed 20 incineration cycles, 30 minutes for each cycle of plastic and cardboard sharps healthcare waste (HCW) containers ranged from 3 to 14...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27909211/application-of-material-flow-analysis-to-municipal-solid-waste-in-maputo-city-mozambique
#9
Leticia Sarmento Dos Muchangos, Akihiro Tokai, Atsuko Hanashima
Understanding waste flows within an urban area is important for identifying the main problems and improvement opportunities for efficient waste management. Assessment tools such as material flow analysis (MFA), an extensively applied method in waste management studies, provide a structured and objective evaluating process to characterize the waste management system best, to identify its shortcomings and to propose suitable strategies. This paper presents the application of MFA to municipal solid waste management (MSWM) in Maputo City, the capital of Mozambique...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27909210/a-full-scale-study-on-thermal-degradation-of-polychlorinated-dibenzo-p-dioxins-and-dibenzofurans-in-municipal-solid-waste-incinerator-fly-ash-and-its-secondary-air-pollution-control-in-china
#10
Xingbao Gao, Bingjing Ji, Dahai Yan, Qifei Huang, Xuemei Zhu
Degradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in municipal solid waste incinerator fly ash is beneficial to its risk control. Fly ash was treated in a full-scale thermal degradation system (capacity 1 t d(-1)) to remove polychlorinated dibenzo-p-dioxins and dibenzofurans. Apart from the confirmation of the polychlorinated dibenzo-p-dioxin and dibenzofuran decomposition efficiency, we focused on two major issues that are the major obstacles for commercialising this decomposition technology in China, desorption and regeneration of dioxins and control of secondary air pollution...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27909209/reuse-of-bituminous-pavements-a-mini-review-of-research-regulations-and-modelling
#11
REVIEW
Joke Anthonissen, Wim Van den Bergh, Johan Braet
Bituminous pavement can be recycled - even multiple times - by reusing it in new bituminous mixtures. If the mechanical properties of the binder get worse, this reclaimed asphalt is often used in the sub-structure of the road. Apparently, up till now, no end-of-life phase exists for the material. Actually, defining the end-of-life and the end-of-waste stage of a material is important for life cycle assessment modelling. Various standards and scientific studies on modelling life cycle assessment are known, but the crucial stages are not yet defined for reclaimed asphalt pavement...
November 30, 2016: Waste Management & Research
https://www.readbyqxmd.com/read/27909143/interobserver-and-intraobserver-reliability-of-clinical-assessments-in-knee-osteoarthritis
#12
Nasimah Maricar, Michael J Callaghan, Matthew J Parkes, David T Felson, Terence W O'Neill
OBJECTIVE: Clinical examination of the knee is subject to measurement error. The aim of this analysis was to determine interobserver and intraobserver reliability of commonly used clinical tests in patients with knee osteoarthritis (OA). METHODS: We studied subjects with symptomatic knee OA who were participants in an open-label clinical trial of intraarticular steroid therapy. Following standardization of the clinical test procedures, 2 clinicians assessed 25 subjects independently at the same visit, and the same clinician assessed 88 subjects over an interval period of 2-10 weeks; in both cases prior to the steroid intervention...
December 2016: Journal of Rheumatology
https://www.readbyqxmd.com/read/27908715/development-and-characterization-of-bacterial-cellulose-reinforced-biocomposite-films-based-on-protein-from-buckwheat-distiller-s-dried-grains
#13
Xuejiao Wang, Niamat Ullah, Xuchun Sun, Yan Guo, Lin Chen, Zhixi Li, Xianchao Feng
Biocomposite films were manufactured by combining protein extracted from buckwheat distiller's dried grains with bacterial cellulose (BC). The film microstructures showed that BC is compatible with protein matrix and endows the film with high rigidity. Differential scanning calorimetry (DSC) showed that BC can promote thermal stability of the composite films. BC promoted the transition from a Newtonian to a non-Newtonian fluid and the shear thinning behavior of protein-BC solution. Fourier Transform Infrared (FTIR) spectroscopy showed the main functional groups' absorption peaks shifted to lower wavenumbers...
November 28, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27908507/usage-of-air-jigging-for-multi-component-separation-of-construction-and-demolition-waste
#14
Weslei Monteiro Ambrós, Carlos Hoffmann Sampaio, Bogdan Grigore Cazacliu, Gerson Luis Miltzarek, Leonardo R Miranda
The use of air jigging for performing multi-component separation in the treatment of mixed construction and demolition waste was studied. Sorting tests were carried out with mixtures of equal bulk volume of concrete and brick in which fixed quantities of unwanted materials - gypsum, wood and paper - were added. Experimental results have demonstrated the possibility to use air jigging to carry out both the removal of low-density contaminants and the concrete concentration in only one process step. In relation to the removal of contaminants only, the overall performance of jigging process can be comparable with that of commercial air classifiers and automatic sorting systems...
November 28, 2016: Waste Management
https://www.readbyqxmd.com/read/27908406/trace-element-biomonitoring-in-hair-of-school-children-from-a-polluted-area-by-sector-field-inductively-coupled-plasma-mass-spectrometry
#15
Evgenii J Drobyshev, Nikolay D Solovyev, Natalya B Ivanenko, Maria Yu Kombarova, Alexander A Ganeev
In the current study, a biomonitoring of 18 hair trace elements (Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, V, Zn, Ca, Na and P) in school children from Leningradskaya Oblast' is reported. A case group, residing in a proximity to the toxic waste disposal grounds (Krasniy Bor), has been assessed vs. controls from a non-urban settlement Seltso. In total, 166 hair samples were analysed using double focusing sector field inductively coupled plasma mass spectrometry after microwave-assisted sample digestion with nitric acid...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27908259/a-novel-liquid-chromatography-tandem-mass-spectrometry-method-for-the-estimation-of-bilirubin-glucuronides-and-its-application-to-in-vitro-enzyme-assays
#16
Siva Prasad Putluru, Murali Krishna Matta, Deepak Ahire, Murali Subramanian, Michael Sinz, Sandhya Mandlekar
Bilirubin is a toxic waste product of metabolism, eliminated mainly through UGT1A1 mediated conjugation to mono- and di-glucuronides. Due to the potentially low Km value of bilirubin glucuronidation, the quantitative sensitivity obtained with most UV/visible light detection methods are not sufficient to accurately calculate UGT1A1 enzyme kinetics and inhibition properties of new chemical entities at low bilirubin concentrations. In addition, bilirubin, as well as its metabolites, are unstable during sample preparation and bioanalysis...
November 24, 2016: Drug Metabolism Letters
https://www.readbyqxmd.com/read/27908201/cocos-nucifera-coir-mediated-green-synthesis-of-pd-nps-and-its-investigation-against-larvae-and-agricultural-pest
#17
Ganesh Elango, Selvaraj Mohana Roopan, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Kasinathan Irukatla Damodharan, Kuppuswamy Elumalai
In recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. Cocos nucifera (C. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. Cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help C. nucifera coir, Palladium nanoparticles (Pd NPs) were synthesized. Green-synthesized spherical-shape Pd NPs were over layered by secondary metabolites from C...
December 1, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27908144/ionic-adsorption-on-the-brucite-0001-surface-a-periodic-electrostatic-embedded-cluster-method-study
#18
Eszter Makkos, Andrew Kerridge, Jonathan Austin, Nikolas Kaltsoyannis
Density functional theory (DFT) at the generalised gradient approximation level is employed within the periodic electrostatic embedded cluster method (PEECM) to model the brucite (0001) surface. Three representative studies are then used to demonstrate the reliability of the PEECM for the description of the interactions of various ionic species with the layered Mg(OH)2 structure, and its performance is compared with periodic DFT, an approach known to be challenging for the adsorption of charged species. The adsorption energies of a series of s block cations, including Sr(2+) and Cs(+) which are known to coexist with brucite in nuclear waste storage ponds, are well described by the embedded cluster model, provided that basis sets of triple-zeta quality are employed for the adsorbates...
November 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27907867/adsorption-characteristics-of-adsorbent-resins-and-antioxidant-capacity-for-enrichment-of-phenolics-from-two-phase-olive-waste
#19
Zhihong Wang, Chengzhang Wang, Jiaojiao Yuan, Changwei Zhang
In this study, the adsorption properties of nine resins including polyamide resin (30-60), polyamide resin (60-100) AB-8, S-8, D-101, NKA-9, NKA-II, XDA-1 and XDA-4 for enrichment phenolics of the olive waste were investigated. XDA-1 and NKA-II were chosen for further study due to their outstanding adsorption and desorption capacity. XDA-1 and NKA-II had similar adsorption and desorption behaviors for phenolics of olive waste. The adsorption mechanism could be better explained by pseudo second-order kinetics model and Freundlich isotherm model, and the adsorption processes were spontaneously and exothermic...
November 24, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27907010/viral-metagenomic-analysis-displays-the-co-infection-situation-in-healthy-and-pmws-affected-pigs
#20
Anne-Lie Blomström, Caroline Fossum, Per Wallgren, Mikael Berg
The development of high-throughput sequencing technologies have allowed the possibility to investigate and characterise the entire microbiome of individuals, providing better insight to the complex interaction between different microorganisms. This will help to understand how the microbiome influence the susceptibility of secondary agents and development of disease. We have applied viral metagenomics to investigate the virome of lymph nodes from Swedish pigs suffering from the multifactorial disease postweaning multisystemic wasting syndrome (PMWS) as well as from healthy pigs...
2016: PloS One
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