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https://www.readbyqxmd.com/read/28631877/identification-of-chemical-components-in-baidianling-capsule-based-on-gc-ms-and-hplc-combined-with-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#1
Wenying Wu, Yu Chen, Binjie Wang, Xiaoyang Sun, Ping Guo, Xiaohui Chen
Baidianling Capsule, which is made from 16 Chinese herbs, has been widely used for treating vitiligo clinically. In this study, the sensitive and rapid method has been developed for the analysis of chemical components in Baidianling Capsule by gas chromatography with mass spectrometry in combination with retention indices and high-performance liquid chromatography combined with Fourier transform-ion cyclotron resonance mass spectrometry. Firstly, a total of 110 potential volatile compounds obtained from different extraction procedures including alkanes, alkenes, alkyne, ketones, ethers, aldehydes, alcohols, phenols, organic acids, esters, furan, pyrrole, acid amide, heterocycle and oxide were detected from Baidianling Capsule by gas chromatography with mass spectrometry, of which 75 were identified by mass spectrometry in combination with the retention index...
June 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28629290/functional-foods-how-functional-are-they-a-case-report-of-supplement-induced-psychosis
#2
Priyanka Yadav, Kyle Stigall, Hannah E Johnson, Abner O Rayapati, Nitin Chopra
There is rising evidence of patients' use of alternative and complementary medicine. The percentage of the U.S. population who used at least one dietary supplement increased from 42% in 1988-1994 to 53% in 2003-2006. We present a case of an Asian female in her 40s, with no previous psychiatric illness, who presented to the emergency room following a brief psychotic episode, during which she self-amputated the tips of her fingers, after using multivitamins and herbal supplements including ginseng, gui yuan rou (Chinese herb), astaxanthin, goji (Chinese fruit), selenium, saw palmetto, grape seed extract, citrus bioflavanoid, lutein (zeaxantin), resvexatrol, sun chlorella, spirulina powder, phytoceramides, phytoestrogen, glucosatrin, bromelain plus, and American bee pollen...
August 2016: International Journal of Psychiatry in Medicine
https://www.readbyqxmd.com/read/28627431/evaluation-of-the-quantamatrix-multiplexed-assay-platform-system-for-simultaneous-detection-of-mycobacteirum-tuberculosis-and-rifampicin-resistance-gene-using-culture-positive-mycobacteria
#3
Hye-Young Wang, Young Uh, Seoyong Kim, Tae-Sun Shim, Hyeyoung Lee
BACKGROUND: The differentiation of Mycobacterium tuberculosis complex (MTBC) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of anti-tuberculosis drugs. Prevailing data on rifampicin (RIF)-resistant TB is essential for early management of multidrug resistant-TB. The aim of this study was to evaluate the usefulness of a newly developed multiplexed, bead-based bioassay (Quantamatrix Multiplexed Assay Platform; QMAP) for the rapid differentiation of 23 Mycobacterium species including MTBC and RIF-resistant strains...
June 13, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28622346/flavivirus-and-filovirus-evoprinters-new-alignment-tools-for-the-comparative-analysis-of-viral-evolution
#4
Thomas Brody, Amarendra S Yavatkar, Dong Sun Park, Alexander Kuzin, Jermaine Ross, Ward F Odenwald
BACKGROUND: Flavivirus and Filovirus infections are serious epidemic threats to human populations. Multi-genome comparative analysis of these evolving pathogens affords a view of their essential, conserved sequence elements as well as progressive evolutionary changes. While phylogenetic analysis has yielded important insights, the growing number of available genomic sequences makes comparisons between hundreds of viral strains challenging. We report here a new approach for the comparative analysis of these hemorrhagic fever viruses that can superimpose an unlimited number of one-on-one alignments to identify important features within genomes of interest...
June 16, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28621744/in-vitro-comparative-study-of-the-inhibitory-effects-of-mangiferin-and-its-aglycone-norathyriol-towards-udp-glucuronosyl-transferase-ugt-isoforms
#5
Dan Sun, Chun-Ze Zhang, Rui-Xue Ran, Yun-Feng Cao, Zuo Du, Zhi-Wei Fu, Chun-Ting Huang, Zhen-Ying Zhao, Wei-Hua Zhang, Zhong-Ze Fang
Mangiferin (MGF), the predominant constituent of extracts of the mango plant Mangifera Indica L., has been investigated extensively because of its remarkable pharmacological effects. In vitro recombinant UGTs-catalyzed glucuronidation of 4-methylumbelliferone (4-MU) was used to investigate the inhibition of mangiferin and aglycone norathyriol towards various isoforms of UGTs in our study, which evaluated the inhibitory capacity of MGF and its aglycone norathyriol (NTR) towards UDP-glucuronosyltransferase (UGT) isoforms...
June 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28621354/density-functional-theory-calculations-for-the-band-gap-and-formation-energy-of-pr4-xcaxsi12o3-xn18-x-a-highly-disordered-compound-with-low-symmetry-and-a-large-cell-size
#6
Sung Un Hong, Satendra Pal Singh, Myoungho Pyo, Woon Bae Park, Kee-Sun Sohn
A novel oxynitride compound, Pr4-xCaxSi12O3+xN18-x, synthesized using a solid-state route has been characterized as a monoclinic structure in the C2 space group using Rietveld refinement on synchrotron powder X-ray diffraction data. The crystal structure of this compound was disordered due to the random distribution of Ca/Pr and N/O ions at various Wyckoff sites. A pragmatic approach for an ab initio calculation based on density function theory (DFT) for this disordered compound has been implemented to calculate an acceptable value of the band gap and formation energy...
June 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28620363/proteomic-analysis-of-trichinella-spiralis-adult-worm-excretory-secretory-proteins-recognized-by-sera-of-patients-with-early-trichinellosis
#7
Zhong Q Wang, Ruo D Liu, Ge G Sun, Yan Y Song, Peng Jiang, Xi Zhang, Jing Cui
The most commonly used serodiagnostic antigens for trichinellosis are the excretory-secretory (ES) antigens from T. spiralis muscle larvae (ML), but the specific antibodies against the ML ES antigens are usually negative during early stage of Trichinella infection. The recent studies demonstrated that T. spiralis adult worm (AW) antigens were recognized by mouse or swine infection sera on Western blot as early as 7-15 days post-infection (dpi), the AW antigens might contain the early diagnostic markers for trichinellosis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28619404/prevalence-of-pediatric-asthma-risk-in-santo-domingo-dominican-republic
#8
Wendy W Sun, Lipi Gupta, Andrew E Andreae, Kristin Romutis, Allison M Borda, Priya Sabu, Sean McKenna, Mark Ryan
BACKGROUND: Early detection and treatment of pediatric asthma could reduce morbidity and lessen burden on society. Currently there is no known research on the prevalence of pediatric asthma in the Dominican Republic (DR) and no known asthma risk assessment tool for one-time encounters in a fast-paced clinic. OBJECTIVES: To pilot a streamlined version of previously validated screening tools to estimate the prevalence of pediatric asthma risk in Santo Domingo Norte, DR...
March 2017: Annals of Global Health
https://www.readbyqxmd.com/read/28619245/the-risk-of-metachronous-neoplasia-on-surveillance-colonoscopy-in-young-patients-with-colorectal-neoplasia
#9
Hyun Gun Kim, Young-Seok Cho, Jae Myung Cha, Jeong Eun Shin, Kyeong Ok Kim, Hyo-Joon Yang, Hoon Sup Koo, Young-Eun Joo, Sun-Jin Boo
BACKGROUND AND AIMS: Few prior reports exist that address the appropriate colonoscopy surveillance interval for individuals <50 years old. We compared the risk of metachronous neoplasia between younger (20-49 years) and older (50-54 years) cohorts. METHODS: This multicenter retrospective cohort study compared the incidence of metachronous neoplasia in younger and older cohorts according to baseline risk stratification. Subjects were eligible if they underwent their first colonoscopy between June 2006 and May 2010, and had at least one or more surveillance colonoscopy up to June 2015...
June 12, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28619043/effect-of-transcutaneous-electrical-stimulation-treatment-on-lower-urinary-tract-symptoms-after-class-iii-radical-hysterectomy-in-cervical-cancer-patients-study-protocol-for-a-multicentre-randomized-controlled-trial
#10
Xiu-Li Sun, Hai-Bo Wang, Zhi-Qi Wang, Ting-Ting Cao, Xin Yang, Jing-Song Han, Yang-Feng Wu, Kathleen H Reilly, Jian-Liu Wang
BACKGROUND: Class III radical hysterectomy (RH III)_plus pelvic lymphadenectomy is the standard surgery for early stage cervical cancer (CC) patients, the 5 year survival rate is about 90%, but pelvic floor disorders especially bladder dysfunction are common due to damaged vessels and nerve fibers following surgery. Transcutaneous electrical stimulation (TENS) treatment has been used to treat bladder disorders for many years, but its effect on cervical cancer patients, the best treatment time point and stimulated protocol, had never been assessed...
June 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28618378/simultaneously-upgrading-biogas-and-purifying-biogas-slurry-using-cocultivation-of-chlorella-vulgaris-and-three-different-fungi-under-various-mixed-light-wavelength-and-photoperiods
#11
Weixing Cao, Xue Wang, Shiqing Sun, Changwei Hu, Yongjun Zhao
In order to purify biogas slurry and biogas simultaneously, three different fungi, Pleurotus geesteranus (P. geesteranus), Ganoderma lucidum (G. lucidum), and Pleurotus ostreatus (P. ostreatus) were pelletized with Chlorella vulgaris (C. vulgaris). The results showed that the optimal light wavelength ratio for red:blue was 5:5 for these three different fungi-assisted C. vulgaris, resulting in higher specific growth rate as well as nutrient and CO2 removal efficiency compared with other ratios. G. lucidum/C...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28618355/chemical-constituents-from-the-fruits-of-ligustrum-japonicum-and-their-inhibitory-effects-on-t-cell-activation
#12
Quynh-Mai Thi Ngo, Hyun-Su Lee, Van Thu Nguyen, Jeong Ah Kim, Mi Hee Woo, Byung Sun Min
A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A-B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production...
June 12, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28617585/solution-phase-photochemical-nanopatterning-enabled-by-high-refractive-index-beam-pen-arrays
#13
Zhuang Xie, Pavlo Gordiichuk, Qing-Yuan Lin, Brian Meckes, Peng-Cheng Chen, Lin Sun, Jingshan S Du, Jinghan Zhu, Yuan Liu, Vinayak P Dravid, Chad A Mirkin
A high-throughput, solution-based, scanning-probe photochemical nanopatterning approach, which does not require the use of probes with subwavelength apertures, is reported. Specifically, pyramid arrays made from high-refractive-index polymeric materials were constructed and studied as patterning tools in a conventional liquid-phase beam pen lithography experiment. Two versions of the arrays were explored with either metal-coated or metal-free tips. Importantly, light can be channeled through both types of tips and the appropriate solution phase (e...
June 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/28615033/a-novel-frameshift-mutation-in-the-xpc-gene-in-a-moroccan-patient-a-case-report
#14
Yassamine Doubaj, Wiam Smaili, Fatima-Zahra Laarabi, Abdelaziz Sefiani
BACKGROUND: Xeroderma pigmentosum is an autosomal recessive inherited disease. The diagnosis is essentially based on clinical findings and the family history. This genodermatosis is genetically heterogeneous; to date, nine genes have been associated to this disorder. Based on the result of many studies, xeroderma pigmentosum complementation group C is the most common form of xeroderma pigmentosum. A founder mutation in the XPC gene was reported in the Maghreb region of northern Africa...
June 15, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28614933/-study-on-the-establishment-of-hmld-model-and-the-expression-of-kl-6-tgf-beta-in-rat
#15
Z S Zhang, Y F Zhao, L Tang, H K Xie, D Y Sun
Objective: To establish an animal model of hard metal lung disease (HMLD) in rats, and to screen the indications for diagnosis of HMLD. Methods: The rats were randomly divided into 5 groups, each group included 8 rats: saline group, pure cobalt group, pure tungsten carbide group, silica group and hard metal (HM) group. 10 mg subjects were administered in each group by using the pulmonary endotracheal tube. After 8 week, the lung CT scan and lung tissue pathology were observed, the serum and bronchoalveolar lavage fluid (BALF) were collected for KL-6, TGF-beta1 and TGF-beta2...
April 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28614913/the-fungal-metabolite-brefeldin-a-inhibits-dvl2-plk1-dependent-primary-cilium-disassembly
#16
Uijeong Lee, Sun-Ok Kim, Jeong-Ah Hwang, Jae-Hyuk Jang, Sangkeun Son, In-Ja Ryoo, Jong Seog Ahn, Bo Yeon Kim, Kyung Ho Lee
The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage (G0 phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases including ciliopathy and cancer. To identify the effective compounds for studying primary cilium disassembly, we have screened 297 natural compounds and identified 18 and 17 primary cilium assembly and disassembly inhibitors, respectively...
June 14, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28614653/microfluidics-enabled-bottom-up-engineering-of-3d-vascularized-tumor-for-drug-discovery
#17
Pranay Agarwal, Hai Wang, Mingrui Sun, Jiangsheng Xu, Shuting Zhao, Zhenguo Liu, Keith J Gooch, Yi Zhao, Xiongbin Lu, Xiaoming He
Development of high-fidelity 3D models to recapitulate the tumor microenvironment is essential for studying tumor biology and screening anticancer drugs. Here we report a method to engineer the 3D microenvironment of human tumor, by encapsulating cancer cells in the core of microcapsules with a hydrogel shell for miniaturized 3D culture to obtain avascular microtumors first. The microtumors are then used as the building blocks for assembling with endothelial cells and other stromal cells to create macroscale 3D vascularized tumor...
June 14, 2017: ACS Nano
https://www.readbyqxmd.com/read/28612559/-the-establishment-and-identification-of-stable-cho-cell-line-with-canine-transferrin-receptor-expression
#18
Wei Zhang, Yan Yan, Lu-Gen Bai, Yan-Ni Zhu, Ling Huang, Yu-Ning Sun
OBJECTIVES: To construct eukaryotic expressing recombinant vector of canine transferrin receptor gene (TfR ), then to transfect Chinese hamster ovary (CHO) cells with the vector for establishment of stable expression of TfR in CHO cell line. METHODS: The full-length TfR fragment was amplified by RT-PCR from the canine cells (walter reed dog cell, WRD) and then inserted into eukaryotic expression vector pCDNA3. After identification with enzyme digestion and sequencing, the recombinant vector was transfected into CHO cells by TransLipid Transfection Reagent...
January 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28611766/establishment-and-application-of-a-high-throughput-screening-system-targeting-the-interaction-between-hcv-internal-ribosome-entry-site-and-human-eukaryotic-translation-initiation-factor-3
#19
Yuying Zhu, Pei Huang, Na Yang, Rui Liu, Xueting Liu, Huanqin Dai, Lixin Zhang, Fuhang Song, Chaomin Sun
Viruses are intracellular obligate parasites and the host cellular machinery is usually recruited for their replication. Human eukaryotic translation initiation factor 3 (eIF3) could be directly recruited by the hepatitis C virus (HCV) internal ribosome entry site (IRES) to promote the translation of viral proteins. In this study, we establish a fluorescence polarization (FP) based high throughput screening (HTS) system targeting the interaction between HCV IRES and eIF3. By screening a total of 894 compounds with this HTS system, two compounds (Mucl39526 and NP39) are found to disturb the interaction between HCV IRES and eIF3...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28611493/construction-selection-and-immunogenicity-of-recombinant-fowlpox-candidate-vaccine-co-expressing-hiv-1-gag-and-gp145
#20
Yilong Zhu, Yan Guo, Shouwen Du, Cunxia Liu, Maopeng Wang, Dayong Ren, Fei Zhao, Yanfang Zhang, Wenchao Sun, Yiquan Li, Tingting Cao, Yingyue Jiang, Bin Xing, Bing Bai, Chang Li, Ningyi Jin
An HIV candidate vaccine for the Chinese population was designed by constructing a recombinant fowlpox virus expressing HIV-1 gag and HIV gp145 proteins via homologous recombination and plaque screening using enhanced green fluorescent protein (EGFP) as the reporter gene. EGFP in the recombinant was then knocked out with the Cre/Loxp system yielding rFPVHg-Hp, which was identified at the genomic, transcriptional and translational levels. The immunogenicity of rFPVHg-Hp was analyzed by measuring levels of HIV-specific antibodies and IFN-γ-secreting splenocytes by enzyme-linked immunosorbent assay and IFN enzyme-linked immune spot test in the BALB/c mouse model...
June 2017: Indian Journal of Microbiology
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