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https://www.readbyqxmd.com/read/28445847/to-elute-or-not-to-elute-in-immunocapture-bottom-up-lc-ms
#1
Maren Christin Stillesby Levernæs, Marianne Nordlund Broughton, Léon Reubsaet, Trine Grønhaug Halvorsen
Immunocapture-based bottom-up LC-MS is a promising technique for the quantification of low abundant proteins. Magnetic immunocapture beads provide efficient enrichment from complex samples through the highly specific interaction between the target protein and its antibody. In this article, we have performed the first thorough comparison between digestion of proteins while bound to antibody coated beads versus after elution from the beads. Two previously validated immunocapture based MS methods for the quantification of pro-gastrin releasing peptide (ProGRP) and human chorionic gonadotropin (hCG) were used as model systems...
April 14, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445834/light-harvesting-antenna-complexes-in-the-moss-physcomitrella-patens-implications-for-the-evolutionary-transition-from-green-algae-to-land-plants
#2
REVIEW
Masakazu Iwai, Makio Yokono
Plants have successfully adapted to a vast range of terrestrial environments during their evolution. To elucidate the evolutionary transition of light-harvesting antenna proteins from green algae to land plants, the moss Physcomitrella patens is ideally placed basally among land plants. Compared to the genomes of green algae and land plants, the P. patens genome codes for more diverse and redundant light-harvesting antenna proteins. It also encodes Lhcb9, which has characteristics not found in other light-harvesting antenna proteins...
April 23, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28445806/patient-engagement-at-the-margins-health-care-providers-assessments-of-engagement-and-the-structural-determinants-of-health-in-the-safety-net
#3
Mark D Fleming, Janet K Shim, Irene Yen, Ariana Thompson-Lastad, Sara Rubin, Meredith Van Natta, Nancy J Burke
Increasing "patient engagement" has become a priority for health care organizations and policy-makers seeking to reduce cost and improve the quality of care. While concepts of patient engagement have proliferated rapidly across health care settings, little is known about how health care providers make use of these concepts in clinical practice. This paper uses 20 months of ethnographic and interview research carried out from 2015 to 2016 to explore how health care providers working at two public, urban, safety-net hospitals in the United States define, discuss, and assess patient engagement...
April 20, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28445768/polymer-free-electrospun-nanofibers-from-sulfobutyl-ether7-beta-cyclodextrin-sbe7-%C3%AE-cd-inclusion-complex-with-sulfisoxazole-fast-dissolving-and-enhanced-water-solubility-of-sulfisoxazole
#4
Zehra Irem Yildiz, Asli Celebioglu, Tamer Uyar
In this study, our aim was to develop solid drug-cyclodextrin inclusion complex system having nanofibrous morphology in order to have fast-dissolving property and enhanced water-solubility of poorly water-soluble drug. Here, we prepared a highly concentrated aqueous solution of inclusion complex between sulfisoxazole and sulfobutyl ether7-beta-cyclodextrin (SBE7-β-CD, Captisol(®)), and then, without using any polymeric matrix, the electrospinning of sulfisoxazole/SBE7-β-CD-IC nanofibers was performed in order to obtain free-standing and handy nanofibrous web...
April 23, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28445747/physical-and-functional-characterization-of-a-viral-genome-maturation-complex
#5
Teng-Chieh Yang, David Ortiz, Qin Yang, Rolando W De Angelis, Saurarshi J Sanyal, Carlos E Catalano
Genome packaging is strongly conserved in the complex double-stranded DNA viruses, including the herpesviruses and many bacteriophages. In these cases, viral DNA is packaged into a procapsid shell by a terminase enzyme. The packaging substrate is typically a concatemer composed of multiple genomes linked in a head-to-tail fashion, and terminase enzymes perform two essential functions: 1) excision of a unit length genome from the concatemer (genome maturation) and 2) translocation of the duplex into a procapsid (genome packaging)...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445735/a-design-principle-for-an-autonomous-post-translational-pattern-formation
#6
Shuhei S Sugai, Koji L Ode, Hiroki R Ueda
Previous autonomous pattern-formation models often assumed complex molecular and cellular networks. This theoretical study, however, shows that a system composed of one substrate with multisite phosphorylation and a pair of kinase and phosphatase can generate autonomous spatial information, including complex stripe patterns. All (de-)phosphorylation reactions are described with a generic Michaelis-Menten scheme, and all species freely diffuse without pre-existing gradients. Computational simulation upon >23,000,000 randomly generated parameter sets revealed the design motifs of cyclic reaction and enzyme sequestration by slow-diffusing substrates...
April 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28445589/a-network-engineering-perspective-on-probing-and-perturbing-cognition-with-neurofeedback
#7
REVIEW
Danielle S Bassett, Ankit N Khambhati
Network science and engineering provide a flexible and generalizable tool set to describe and manipulate complex systems characterized by heterogeneous interaction patterns among component parts. While classically applied to social systems, these tools have recently proven to be particularly useful in the study of the brain. In this review, we describe the nascent use of these tools to understand human cognition, and we discuss their utility in informing the meaningful and predictable perturbation of cognition in combination with the emerging capabilities of neurofeedback...
April 26, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28445442/a-review-study-on-macrolides-isolated-from-cyanobacteria
#8
REVIEW
Mengchuan Wang, Jinrong Zhang, Shan He, Xiaojun Yan
Cyanobacteria are rich sources of structurally-diverse molecules with promising pharmacological activities. Marine cyanobacteria have been proven to be true producers of some significant bioactive metabolites from marine invertebrates. Macrolides are a class of bioactive compounds isolated from marine organisms, including marine microorganisms in particular. The structural characteristics of macrolides from cyanobacteria mainly manifest in the diversity of carbon skeletons, complexes of chlorinated thiazole-containing molecules and complex spatial configuration...
April 26, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28445403/detection-of-hepatitis-b-virus-m204i-mutation-by-quantum-dot-labeled-dna-probe
#9
Cheng Zhang, Yiping Chen, Xinmiao Liang, Guanhua Zhang, Hong Ma, Leng Nie, Yu Wang
Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy...
April 26, 2017: Sensors
https://www.readbyqxmd.com/read/28445399/advances-in-psychiatric-diagnosis-past-present-and-future
#10
EDITORIAL
Carol S North, Alina M Surís
This editorial examines controversies identified by the articles in this special issue, which explore psychopathology in the broad history of the classification of selected psychiatric disorders and syndromes over time through current American criteria. Psychiatric diagnosis has a long history of scientific investigation and application, with periods of rapid change, instability, and heated controversy associated with it. The articles in this issue examine the history of psychiatric nomenclature and explore current and future directions in psychiatric diagnosis through the various versions of accepted diagnostic criteria and accompanying research literature addressing the criteria...
April 26, 2017: Behavioral Sciences
https://www.readbyqxmd.com/read/28444961/biodiversity-and-ecosystem-function-making-sense-of-numerous-species-interactions-in-multi-species-communities
#11
Caroline Brophy, Áine Dooley, Laura Kirwan, John A Finn, Jack McDonnell, Thomas Bell, Marc W Cadotte, John Connolly
Understanding the biodiversity and ecosystem function relationship can be challenging in species-rich ecosystems. Traditionally, species richness has been relied on heavily to explain changes in ecosystem function across diversity gradients. Diversity-Interactions models can test how ecosystem function is affected by species identity, species interactions and evenness, in addition to richness. However, in a species-rich system, there may be too many species interactions to allow estimation of each coefficient, and if all interaction coefficients are estimable, they may be devoid of any sensible biological meaning...
April 26, 2017: Ecology
https://www.readbyqxmd.com/read/28444903/electrospray-ionization-mass-spectrometry-for-the-hydrolysis-complexes-of-cisplatin-implications-for-the-hydrolysis-process-of-platinum-complexes
#12
Feifan Xie, Pieter Colin, Jan Van Bocxlaer
Non-enzyme dependent hydrolysis of the drug cisplatin is important for its mode of action and toxicity. However, up until today, the hydrolysis process of cisplatin is still not completely understood. In the present study, the hydrolysis of cisplatin in an aqueous solution was systematically investigated using electrospray ionization mass spectrometry coupled to liquid chromatography. A variety of previously unreported hydrolysis complexes corresponding to mono-, di-, and trimeric species were detected and identified...
April 26, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28444887/morphological-study-of-the-atrioventricular-conduction-system-and-purkinje-fibers-in-yak
#13
Deyong Duan, Sijiu Yu, Yan Cui, Chaoxu Li
We studied the morphology of the atrioventricular conduction system (AVCS) and Purkinje fibers of the yak. Light and transmission electron microscopy were used to study the histological features of AVCS. The distributional characteristics of the His-bundle, the left bundle branch (LBB), right bundle branch (RBB), and Purkinje fiber network of yak hearts were examined using gross dissection, ink injection, and ABS casting. The results showed that the atrioventricular node (AVN) of yak located in the right side of interatrial septum and had a flattened ovoid shape...
April 26, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28444833/chronic-stress-induced-mechanical-hyperalgesia-is-controlled-by-capsaicin-sensitive-neurones-in-the-mouse
#14
B Scheich, P Vincze, É Szőke, É Borbély, Á Hunyady, J Szolcsányi, Á Dénes, Zs Környei, B Gaszner, Zs Helyes
BACKGROUND: Clinical studies demonstrated peripheral nociceptor deficit in stress-related chronic pain states, such as fibromyalgia. The interactions of stress and nociceptive systems have special relevance in chronic pain, but the underlying mechanisms including the role of specific nociceptor populations remain unknown. We investigated the role of capsaicin-sensitive neurones in chronic stress-related nociceptive changes. METHOD: Capsaicin-sensitive neurones were desensitized by the capsaicin analogue resiniferatoxin (RTX) in CD1 mice...
April 25, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28444733/hybridization-increases-mitochondrial-production-of-reactive-oxygen-species-in-sunfish
#15
Sherry N N Du, Fariborz Khajali, Neal J Dawson, Graham R Scott
Mitochondrial dysfunction and oxidative stress have been suggested to be possible mechanisms underlying hybrid breakdown, as a result of mito-nuclear incompatibilities in respiratory complexes of the electron transport system. However, it remains unclear whether hybridization increases the production of reactive oxygen species (ROS) by mitochondria. We used high-resolution respirometry and fluorometry on isolated liver mitochondria to examine mitochondrial physiology and ROS emission in naturally occurring hybrids of pumpkinseed (Lepomis gibbosus) and bluegill (L...
April 26, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28444700/fluorescence-lifetime-imaging-and-super-resolution-microscopies-shed-light-on-the-directed-and-self-assembly-of-functional-porphyrins-onto-carbon-nanotubes-and-flat-surfaces
#16
Boyang Mao, David González Calatayud, Vincenzo Mirabello, Haobo Ge, Benjamin J Hodges, Robert M J Jacobs, Ashley M Shepherd, José Alberto Ribeiro Martins, Navaratnarajah Kuganathan, Jorge Bernardino De La Serna, Stanley W Botchway, Sofia Ioana Pascu
Two new routes to functionalized SWNTs have been established using a bulky Zn(II)-porphyrin featuring thiolate groups at the periphery. We probed the optical properties of this Zinc(II)-substituted, bulky aryl porphyrin and those of the corresponding new nano-composites with single walled carbon nanotube (SWNTs) and coronene, as a model for graphene. We report hereby on: (i) the supramolecular interactions between the pristine SWNTs and Zn(II)-porphyrin by virtue of π-π stacking, and (ii) a novel covalent binding strategy based on the Bingel reaction...
April 25, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28444399/development-of-a-multipurpose-scaffold-for-the-display-of-peptide-loops
#17
Maxim Rossmann, Sandra J Greive, Tommaso Moschetti, Michael Dinan, Marko Hyvönen
Protein-protein interactions (PPIs) determine a wide range of biological processes and analysis of these dynamic networks is increasingly becoming a mandatory tool for studying protein function. Using the globular ATPase domain of recombinase RadA as a scaffold, we have developed a peptide display system (RAD display), which allows for the presentation of target peptides, protein domains or full-length proteins and their rapid recombinant production in bacteria. The design of the RAD display system includes differently tagged versions of the scaffold, which allows for flexibility in the protein purification method, and chemical coupling for small molecule labeling or surface immobilization...
April 24, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28444192/from-reproducibility-to-translation-in-neurodegenerative-disease
#18
Caroline J Zeiss
Despite tremendous investment and preclinical success in neurodegenerative disease, effective disease-altering treatments for patients have remained elusive. One highly cited reason for this discrepancy is flawed animal study design and reporting. If this can be broadly remedied, reproducibility of preclinical studies will improve. However, without concurrent efforts to improve generalizability, these improvements may not translate effectively from animal experiments to more complex human neurodegenerative diseases...
April 21, 2017: ILAR Journal
https://www.readbyqxmd.com/read/28444170/neuronal-activity-tgf%C3%AE-signaling-and-unpredictable-chronic-stress-modulate-transcription-of-gadd45-family-members-and-dna-methylation-in-the-hippocampus
#19
Daniela Grassi, Henriette Franz, Riccardo Vezzali, Patrick Bovio, Stefanie Heidrich, Fariba Dehghanian, Natalia Lagunas, Catherine Belzung, Kerstin Krieglstein, Tanja Vogel
Neuronal activity is altered in several neurological and psychiatric diseases. Upon depolarization not only neurotransmitters are released but also cytokines and other activators of signaling cascades. Unraveling their complex implication in transcriptional control in receiving cells will contribute to understand specific central nervous system (CNS) pathologies and will be of therapeutically interest. In this study we depolarized mature hippocampal neurons in vitro using KCl and revealed increased release not only of brain-derived neurotrophic factor (BDNF) but also of transforming growth factor beta (TGFB)...
April 21, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28444127/hla-class-i-binding-prediction-via-convolutional-neural-networks
#20
Yeeleng S Vang, Xiaohui Xie
Motivation: Many biological processes are governed by protein-ligand interactions. One such example is the recognition of self and nonself cells by the immune system. This immune response process is regulated by the major histocompatibility complex (MHC) protein which is encoded by the human leukocyte antigen (HLA) complex. Understanding the binding potential between MHC and peptides can lead to the design of more potent, peptide-based vaccines and immunotherapies for infectious autoimmune diseases...
April 21, 2017: Bioinformatics
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