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"Cardiac patch"

Tadahisa Sugiura, Narutoshi Hibino, Christopher K Breuer, Toshiharu Shinoka
BACKGROUND: Thousands of babies are born with congenital heart defects that require surgical repair involving a prosthetic implant. Lack of growth in prosthetic grafts is especially detrimental in pediatric surgery. Cell seeded biodegradable tissue engineered grafts are a novel solution to this problem. The purpose of the present study is to evaluate the feasibility of seeding human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) onto a biodegradable cardiac patch. METHODS: The hiPS-CMs were cultured on a biodegradable patch composed of a polyglycolic acid (PGA) and a 50:50 poly (l-lactic-co-ε-caprolactone) copolymer (PLCL) for 1 week...
December 1, 2016: Journal of Cardiothoracic Surgery
Elisa Avolio, Valeria V Alvino, Mohamed T Ghorbel, Paola Campagnolo
The recent development of tissue engineering provides exciting new perspectives for the replacement of failing organs and the repair of damaged tissues. Perivascular cells, including vascular smooth muscle cells, pericytes and other tissue specific populations residing around blood vessels, have been isolated from many organs and are known to participate to the in situ repair process and angiogenesis. Their potential has been harnessed for cell therapy of numerous pathologies; however, in this Review we will discuss the potential of perivascular cells in the development of tissue engineering solutions for healthcare...
November 23, 2016: Pharmacology & Therapeutics
Y Murat Elçin, Arin Dogan, Eser Elçin
Cardiovascular diseases are the leading cause of worldwide deaths. Current paradigm in medicine seeks novel approaches for the treatment of progressive or end-stage diseases. The organ transplantation option is limited in availability and unfortunately, a great number of patients are lost while waiting for donor organs. Animal studies have shown that upon myocardial infarction, it may be possible to stop adverse remodeling in its tracks and reverse with tissue engineering methods. Regaining the myocardium function and avoiding further deterioration towards heart failure can benefit millions of people with a significantly lesser burden on healthcare systems worldwide...
November 11, 2016: Current Pharmaceutical Design
Andrea V Bagdadi, Maryam Safari, Prachi Dubey, Pooja Basnett, Panagiotis Sofokleous, Eleanor Humphrey, Ian Locke, Mohan Edirisinghe, Cesare Terracciano, Aldo R Boccaccini, Jonathan C Knowles, Sian E Harding, Ipsita Roy
Cardiac tissue engineering (CTE) is currently a prime focus of research due to an enormous clinical need. In this work, a novel functional material, Poly(3-hydroxyoctanoate), P(3HO), a medium chain length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mechanical properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topography, to allow efficient cell adhesion and proliferation, were fabricated...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
Tze-Wen Chung, Hsin-Yu Lo, Tzung-Han Chou, Jan-Hou Chen, Shoei-Shen Wang
Bone marrow-derived mesenchymal stem cell microtissues (BMSCMT) enhanced cardiomyogenesis in vitro and cardiac repairs of myocardial infarcted hearts in vivo are documented. Producing human BMSCMT onto patches in vitro for cardiac tissue engineering has not been reported. For possibly producing human bone marrow-derived mesenchymal stem cell microtissues (hBMSCMT) on an elastic silk fibroin (SF)-poly(ε-caprolactone) (PCL) based patches is hereby designed. After an elastic SF-PCL (SP) patch is fabricated, hyaluronic acid (HA)/SF-PCL(HSP) and HA-GRGD/SF-PCL(HGSP) patches are fabricated by photochemically grafting HA and HA-GRGD onto SP surfaces...
September 28, 2016: Macromolecular Bioscience
Akiko Tanaka, Keigo Kawaji, Amit R Patel, Takeyoshi Ota
OBJECTIVES: An extracellular matrix patch was implanted in the porcine right ventricle for in situ myocardial regeneration. A newly developed cardiovascular magnetic resonance protocol was utilized to investigate the regional physio-mechanical function of the patch. METHODS: Cardiovascular magnetic resonance was performed at 60-day after the porcine right ventricular wall full thickness substitution with an extracellular matrix cardiac patch (n = 5). Dacron patches and remote normal right ventricle served as control (n = 5/each)...
September 13, 2016: Interactive Cardiovascular and Thoracic Surgery
Jonathan Rodness, Anton Mihic, Yasuo Miyagi, Jun Wu, Richard D Weisel, Ren-Ke Li
BACKGROUND: Revascularization of the heart after myocardial infarction (MI) using growth factors delivered by hydrogel-based microspheres represents a promising therapeutic approach for cardiac regeneration. Microspheres have tuneable degradation properties and support the prolonged release of soluble factors. Cardiac patches provide mechanical restraint, preventing dilatation associated with ventricular remodelling. METHODS: We combined these approaches and produced a compacted calcium-alginate microsphere patch, restrained by a chitosan sheet, to deliver vascular endothelial growth factor (VEGF) to the heart after myocardial injury in rats...
November 2016: Acta Biomaterialia
M Tallawi, D Dippold, R Rai, D D'Atri, J A Roether, D W Schubert, E Rosellini, F B Engel, A R Boccaccini
Nano- and micro-scale topographical features play a critical role in the induction and maintenance of various cellular properties and functions, including morphology, adhesion, gene regulation, and cell-to-cell communication. In addition, recent studies have indicated that the structure and function of heart tissue are also sensitive to mechanical cues at the nano- and micro-scale. Although fabrication methods exist for generating topographical features on polymeric scaffolds for cell culture, current techniques, especially those with nano-scale resolution, are typically complex, prohibitively expensive and not accessible to most biology laboratories...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Antonio D'Amore, Tomo Yoshizumi, Samuel K Luketich, Matthew T Wolf, Xinzhu Gu, Marcello Cammarata, Richard Hoff, Stephen F Badylak, William R Wagner
As an intervention to abrogate ischemic cardiomyopathy, the concept of applying a temporary, local patch to the surface of the recently infarcted ventricle has been explored from a number of design perspectives. Two important features considered for such a cardiac patch include the provision of appropriate mechanical support and the capacity to influence the remodeling pathway by providing cellular or biomolecule delivery. The objective of this report was to focus on these two features by first evaluating the incorporation of a cardiac extracellular matrix (ECM) component, and second by evaluating the impact of patch anisotropy on the pathological remodeling process initiated by myocardial infarction...
November 2016: Biomaterials
Qingjie Wang, Hui Yang, Aobing Bai, Wei Jiang, Xiuya Li, Xinhong Wang, Yishen Mao, Chao Lu, Ruizhe Qian, Feng Guo, Tianling Ding, Haiyan Chen, Sifeng Chen, Jianyi Zhang, Chen Liu, Ning Sun
With the advent of induced pluripotent stem cells and directed differentiation techniques, it is now feasible to derive individual-specific cardiac cells for human heart tissue engineering. Here we report the generation of functional engineered human cardiac patches using human induced pluripotent stem cells-derived cardiac cells and decellularized natural heart ECM as scaffolds. The engineered human cardiac patches can be tailored to any desired size and shape and exhibited normal contractile and electrical physiology in vitro...
October 2016: Biomaterials
Joseph J Kim, Luqia Hou, Ngan F Huang
UNLABELLED: Engineering of three-dimensional (3D) tissues is a promising approach for restoring diseased or dysfunctional myocardium with a functional replacement. However, a major bottleneck in this field is the lack of efficient vascularization strategies, because tissue constructs produced in vitro require a constant flow of oxygen and nutrients to maintain viability and functionality. Compared to angiogenic cell therapy and growth factor treatment, bioengineering approaches such as spatial micropatterning, integration of sacrificial materials, tissue decellularization, and 3D bioprinting enable the generation of more precisely controllable neovessel formation...
September 1, 2016: Acta Biomaterialia
Ali Navaei, Harpinder Saini, Wayne Christenson, Ryan Tanner Sullivan, Robert Ros, Mehdi Nikkhah
UNLABELLED: The development of advanced biomaterials is a crucial step to enhance the efficacy of tissue engineering strategies for treatment of myocardial infarction. Specific characteristics of biomaterials including electrical conductivity, mechanical robustness and structural integrity need to be further enhanced to promote the functionalities of cardiac cells. In this work, we fabricated UV-crosslinkable gold nanorod (GNR)-incorporated gelatin methacrylate (GelMA) hybrid hydrogels with enhanced material and biological properties for cardiac tissue engineering...
September 1, 2016: Acta Biomaterialia
Yang Liu, Yachen Xu, Zhenhua Wang, Dezhong Wen, Wentian Zhang, Sebastian Schmull, Haiyan Li, Yao Chen, Song Xue
Electrospun nanofibrous sheets get increasing attention in myocardial infarction (MI) treatment due to their good cytocompatibility to deliver transplanted stem cells to infarcted areas and due to mechanical characteristics to support damaged tissue. Cardiac extracellular matrix is essential for implanted cells since it provides the cardiac microenvironment. In this study, we hypothesized high concentrations of cardiac nature protein (NP), namely elastin and collagen, in hybrid polycaprolactone (PCL) electrospun nanofibrous sheets could be effective as cardiac-mimicking patch...
2016: American Journal of Translational Research
Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
No abstract text is available yet for this article.
June 2016: Journal of Biomedical Materials Research. Part A
Chia Wei Chang, Tye Petrie, Alycia Clark, Xin Lin, Claus S Sondergaard, Leigh G Griffiths
In this study, we investigate the translational potential of a novel combined construct using an FDA-approved decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM) seeded with human or porcine mesenchymal stem cells (MSCs) for cardiovascular indications. With the emerging success of individual component in various clinical applications, the combination of SIS-ECM with MSCs could provide additional therapeutic potential compared to individual components alone for cardiovascular repair...
2016: PloS One
Ron Feiner, Leeya Engel, Sharon Fleischer, Maayan Malki, Idan Gal, Assaf Shapira, Yosi Shacham-Diamand, Tal Dvir
In cardiac tissue engineering approaches to treat myocardial infarction, cardiac cells are seeded within three-dimensional porous scaffolds to create functional cardiac patches. However, current cardiac patches do not allow for online monitoring and reporting of engineered-tissue performance, and do not interfere to deliver signals for patch activation or to enable its integration with the host. Here, we report an engineered cardiac patch that integrates cardiac cells with flexible, freestanding electronics and a 3D nanocomposite scaffold...
June 2016: Nature Materials
Ashkan Shafiee, Anthony Atala
Over the past 15 years, printers have been increasingly utilized for biomedical applications in various areas of medicine and tissue engineering. This review discusses the current and future applications of 3D bioprinting. Several 3D printing tools with broad applications from surgical planning to 3D models are being created, such as liver replicas and intermediate splints. Numerous researchers are exploring this technique to pattern cells or fabricate several different tissues and organs, such as blood vessels or cardiac patches...
March 2016: Trends in Molecular Medicine
Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale, Gianluigi Condorelli
In first part of this experiment, biocompatibility of the newly developed electroactive polyurethane/siloxane films containing aniline tetramer moieties was demonstrated with proliferation and differentiation of C2C12 myoblasts. Here we further assessed the cytocompatibility of the prepared samples with HL1-cell line, the electrophysiological properties and the patch clamp recording of the seeded cells over the selected electroactive sample. Presence of electroactive aniline tetramer in the structure of polyurethane/siloxane led to the increased expression of cardiac-specific genes of HL-1 cells involved in muscle contraction and electrical coupling...
June 2016: Journal of Biomedical Materials Research. Part A
Fabrizio Del Bianco, Piero Colli Franzone, Simone Scacchi, Lorenzo Fassina
Today, in vitro cardiac cultures are widely exploited to investigate several aspects of the electromechanical behavior of the cardiac tissue. Thus, new forecasts may derive from modelling their properties. In particular, in this paper, we focus on the fiber architecture of cultures, i.e. on the way cellular sarcomeres are locally oriented, when they are designed to be cardiac patches. We employ a three-dimensional model to simulate the bioelectrical activity and the biomechanics of a multilayered culture made of ventricular cells and with four possible architectures consisting of: i) random fibers in all cells; ii) randomly rotating fibers among layers; iii) structurally rotating fibers from the bottom layer to the top one; iv) parallel fibers among layers...
2015: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Fabrizio Del Bianco, Piero Colli Franzone, Simone Scacchi, Lorenzo Fassina
Nowadays, in vitro cardiac cultures offer a valid tool to study the bioelectrical activity and the biomechanics of the heart tissue. Modelling their properties could be helpful for researchers involved in this field. In this paper, we develop a three-dimensional electromechanical model to study how thickness affects the bioelectrical and biomechanical performances of an in vitro culture made of ventricular cells. In particular, by our in silico simulations we want to verify if thickness variations can be a key factor in modifying the response of the whole culture when this one is grown to become a cardiac patch...
2015: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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