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"Cardiac patch"

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https://www.readbyqxmd.com/read/28805824/flexible-shape-memory-scaffold-for-minimally-invasive-delivery-of-functional-tissues
#1
Miles Montgomery, Samad Ahadian, Locke Davenport Huyer, Mauro Lo Rito, Robert A Civitarese, Rachel D Vanderlaan, Jun Wu, Lewis A Reis, Abdul Momen, Saeed Akbari, Aric Pahnke, Ren-Ke Li, Christopher A Caldarone, Milica Radisic
Despite great progress in engineering functional tissues for organ repair, including the heart, an invasive surgical approach is still required for their implantation. Here, we designed an elastic and microfabricated scaffold using a biodegradable polymer (poly(octamethylene maleate (anhydride) citrate)) for functional tissue delivery via injection. The scaffold's shape memory was due to the microfabricated lattice design. Scaffolds and cardiac patches (1 cm × 1 cm) were delivered through an orifice as small as 1 mm, recovering their initial shape following injection without affecting cardiomyocyte viability and function...
August 14, 2017: Nature Materials
https://www.readbyqxmd.com/read/28715377/creation-of-cardiac-tissue-exhibiting-mechanical-integration-of-spheroids-using-3d-bioprinting
#2
Chin Siang Ong, Takuma Fukunishi, Andrew Nashed, Adriana Blazeski, Huaitao Zhang, Samantha Hardy, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon Tomaselli, Narutoshi Hibino
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates. Within 3 days, beating spheroids form. These spheroids are then picked up by a nozzle using vacuum suction and assembled on a needle array using a 3D bioprinter. The spheroids are then allowed to fuse on the needle array...
July 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28676704/biomaterial-free-three-dimensional-bioprinting-of-cardiac-tissue-using-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#3
Chin Siang Ong, Takuma Fukunishi, Huaitao Zhang, Chen Yu Huang, Andrew Nashed, Adriana Blazeski, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon F Tomaselli, Narutoshi Hibino
We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28664892/potential-of-propagation-based-synchrotron-x-ray-phase-contrast-computed-tomography-for-cardiac-tissue-engineering
#4
Mohammad Izadifar, Paul Babyn, Dean Chapman, Michael E Kelly, Xiongbiao Chen
Hydrogel-based cardiac tissue engineering offers great promise for myocardial infarction repair. The ability to visualize engineered systems in vivo in animal models is desired to monitor the performance of cardiac constructs. However, due to the low density and weak X-ray attenuation of hydrogels, conventional radiography and micro-computed tomography are unable to visualize the hydrogel cardiac constructs upon their implantation, thus limiting their use in animal systems. This paper presents a study on the optimization of synchrotron X-ray propagation-based phase-contrast imaging computed tomography (PCI-CT) for three-dimensional (3D) visualization and assessment of the hydrogel cardiac patches...
July 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28433936/sustained-viral-gene-delivery-from-a-micro-fibrous-elastomeric-cardiac-patch-to-the-ischemic-rat-heart
#5
Xinzhu Gu, Yasumoto Matsumura, Ying Tang, Souvik Roy, Richard Hoff, Bing Wang, William R Wagner
Biodegradable and elastomeric patches have been applied to the surface of infarcted hearts as temporary mechanical supports to effectively alter adverse left ventricular remodeling processes. In this report, recombinant adeno-associated virus (AAV), known for its persistent transgene expression and low pathogenicity, was incorporated into elastomeric polyester urethane urea (PEUU) and polyester ether urethane urea (PEEUU) and processed by electrospinning into two formats (solid fibers and core-sheath fibers) designed to influence the controlled release behavior...
April 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28372282/stem-cells-cardiac-patch-from-decellularized-umbilical-artery-improved-heart-function-after-myocardium-infarction
#6
Na Li, RanRan Huang, XiaoXia Zhang, Yi Xin, Jia Li, YiMin Huang, Wei Cui, Jean-Francois Stoltz, YuJie Zhou, QingYu Kong
The construction of the high biocompatible biomaterials pretreated with MSC offers a promising strategy to improve the effects of stem cell therapy for the myocardial infarction (MI). However, assembling vascularized three-dimensional (3-D) myocardial tissues remains an enormous challenge. In this study, we optimized the decellularization protocol with the umbilical artery to construct microporous 3-D scaffold which is suitable for the stem cells (SC) proliferation. The SD rats underwent proximal left coronary ligation and a 5-mm diameter microporous SC patch was implanted directly on the infarct area (SC patch group)...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28322510/rational-design-of-a-conductive-collagen-heart-patch
#7
Peter C Sherrell, Artur Cieślar-Pobuda, Malin Silverå Ejneby, Laura Sammalisto, Amy Gelmi, Ebo de Muinck, Johan Brask, Marek J Łos, Mehrdad Rafat
Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting and repairing the cardiac scar tissue caused by myocardial infarction. Creating cell supportive scaffolds that can be directly implanted on a myocardial infarct is an attractive solution. Hydrogels made of collagen are highly biocompatible materials that can be molded into a range of shapes suitable for cardiac patch applications...
July 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28244640/mesenchymal-stem-cell-loaded-cardiac-patch-promotes-epicardial-activation-and-repair-of-the-infarcted-myocardium
#8
Qiang-Li Wang, Hai-Jie Wang, Zhi-Hua Li, Yong-Li Wang, Xue-Ping Wu, Yu-Zhen Tan
Cardiac patch is considered a promising strategy for enhancing stem cell therapy of myocardial infarction (MI). However, the underlying mechanisms for cardiac patch repairing infarcted myocardium remain unclear. In this study, we investigated the mechanisms of PCL/gelatin patch loaded with MSCs on activating endogenous cardiac repair. PCL/gelatin patch was fabricated by electrospun. The patch enhanced the survival of the seeded MSCs and their HIF-1α, Tβ4, VEGF and SDF-1 expression and decreased CXCL14 expression in hypoxic and serum-deprived conditions...
February 28, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28194397/a-novel-human-tissue-engineered-3-d-functional-vascularized-cardiac-muscle-construct
#9
Mani T Valarmathi, John W Fuseler, Jeffrey M Davis, Robert L Price
Organ tissue engineering, including cardiovascular tissues, has been an area of intense investigation. The major challenge to these approaches has been the inability to vascularize and perfuse the in vitro engineered tissue constructs. Attempts to provide oxygen and nutrients to the cells contained in the biomaterial constructs have had varying degrees of success. The aim of this current study is to develop a three-dimensional (3-D) model of vascularized cardiac tissue to examine the concurrent temporal and spatial regulation of cardiomyogenesis in the context of postnatal de novo vasculogenesis during stem cell cardiac regeneration...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28186692/targeted-genome-engineering-to-control-vegf-expression-in-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-potential-implications-for-the-treatment-of-myocardial-infarction
#10
Hyun-Min Cho, Pyung-Hwan Kim, Hyun-Kyung Chang, Yi-Ming Shen, Kwaku Bonsra, Byung-Jae Kang, Soo-Young Yum, Joo-Hyun Kim, So-Yeong Lee, Min-Cheol Choi, Hyongbum Henry Kim, Goo Jang, Je-Yoel Cho
Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) exhibit potency for the regeneration of infarcted hearts. Vascular endothelial growth factor (VEGF) is capable of inducing angiogenesis and can boost stem cell-based therapeutic effects. However, high levels of VEGF can cause abnormal blood vessel growth and hemangiomas. Thus, a controllable system to induce therapeutic levels of VEGF is required for cell therapy. We generated an inducible VEGF-secreting stem cell (VEGF/hUCB-MSC) that controls the expression of VEGF and tested the therapeutic efficacy in rat myocardial infarction (MI) model to apply functional stem cells to MI...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28167795/modular-assembly-of-thick-multifunctional-cardiac-patches
#11
Sharon Fleischer, Assaf Shapira, Ron Feiner, Tal Dvir
In cardiac tissue engineering cells are seeded within porous biomaterial scaffolds to create functional cardiac patches. Here, we report on a bottom-up approach to assemble a modular tissue consisting of multiple layers with distinct structures and functions. Albumin electrospun fiber scaffolds were laser-patterned to create microgrooves for engineering aligned cardiac tissues exhibiting anisotropic electrical signal propagation. Microchannels were patterned within the scaffolds and seeded with endothelial cells to form closed lumens...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28131907/3d-late-gadolinium-enhanced-cardiovascular-mr-with-centra-plus-profile-view-ordering-feasibility-of-right-ventricular-myocardial-damage-assessment-using-a-swine-animal-model
#12
Keigo Kawaji, Akiko Tanaka, Mita B Patel, Hui Wang, Francesco Maffessanti, Takeyoshi Ota, Amit R Patel
AIMS: To develop a high-resolution, 3D late gadolinium enhancement (LGE) cardiovascular magnetic resonance imaging (MRI) technique for improved assessment of myocardial scars, and evaluate its performance against 2D breath-held (BH) LGE MRI using a surgically implanted animal scar model in the right ventricle (RV). METHODS AND RESULTS: A k-space segmented 3D LGE acquisition using CENTRA-PLUS (Contrast ENhanced Timing Robust Acquisition with Preparation of LongitUdinal Signal; or CP) ordering is proposed...
June 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28068869/a-regenerative-cardiac-patch-formed-by-spray-painting-of-biomaterials-onto-the-heart
#13
Junnan Tang, Adam Vandergriff, Zegen Wang, Michael Taylor Hensley, Jhon Cores, Tyler A Allen, Phuong-Uyen Dinh, Jinying Zhang, Thomas George Caranasos, Ke Cheng
Layering a regenerative polymer scaffold on the surface of the heart, termed as a cardiac patch, has been proven to be effective in preserving cardiac function after myocardial infarction (MI). However, the placement of such a patch on the heart usually needs open-chest surgery, which is traumatic, therefore prevents the translation of this strategy into the clinic. We sought to device a way to apply a cardiac patch by spray painting in situ polymerizable biomaterials onto the heart with a minimally invasive procedure...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28029762/prevascularization-of-decellularized-porcine-myocardial-slice-for-cardiac-tissue-engineering
#14
Pawan Kc, Mickey Shah, Jun Liao, Ge Zhang
Prevacularization strategies have been implemented in tissue engineering to generate microvasculature networks within a scaffold prior to implantation. Prevascularizing scaffolds will shorten the time of functional vascular perfusion with host upon implantation. In this study, we explored key variables affecting the interaction between decellularized porcine myocardium slices (dPMSs) and reseeded stem cells toward the fabrication of prevascularized cardiac tissue. Our results demonstrated that dPMS supports attachment of human mesenchymal stem cells (hMSCs) and rat adipose derived stem cells (rASCs) with high viability...
January 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27943590/a-collagen-cardiac-patch-incorporating-alginate-microparticles-permits-the-controlled-release-of-hepatocyte-growth-factor-and-insulin-like-growth-factor-1-to-enhance-cardiac-stem-cell-migration-and-proliferation
#15
Hugh S O'Neill, Janice O'Sullivan, Niamh Porteous, Eduardo Ruiz-Hernandez, Helena M Kelly, Fergal J O'Brien, Garry P Duffy
Cardiac stem cells (CSCs) represent a logical cell type to exploit as a regenerative treatment option for tissue damage accrued as a result of a myocardial infarction. However, the isolation and expansion of CSCs prior to cell transplantation is time consuming, costly and invasive, and the reliability of cell expansion may also prove to be a major obstacle in the clinical application of CSC-based transplantation therapy after a myocardial infarction. In order to overcome this, we propose the incorporation of growth factor-eluting alginate microparticles into collagen-based scaffolds as an implantable biomaterial to promote the recruitment and expansion of CSCs in the myocardium...
December 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27906085/tissue-engineered-cardiac-patch-seeded-with-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-promoted-the-regeneration-of-host-cardiomyocytes-in-a-rat-model
#16
Tadahisa Sugiura, Narutoshi Hibino, Christopher K Breuer, Toshiharu Shinoka
BACKGROUND: Thousands of babies are born with congenital heart defects that require surgical repair involving a prosthetic implant. Lack of growth in prosthetic grafts is especially detrimental in pediatric surgery. Cell seeded biodegradable tissue engineered grafts are a novel solution to this problem. The purpose of the present study is to evaluate the feasibility of seeding human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) onto a biodegradable cardiac patch. METHODS: The hiPS-CMs were cultured on a biodegradable patch composed of a polyglycolic acid (PGA) and a 50:50 poly (l-lactic-co-ε-caprolactone) copolymer (PLCL) for 1 week...
December 1, 2016: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/27889329/perivascular-cells-and-tissue-engineering-current-applications-and-untapped-potential
#17
REVIEW
Elisa Avolio, Valeria V Alvino, Mohamed T Ghorbel, Paola Campagnolo
The recent development of tissue engineering provides exciting new perspectives for the replacement of failing organs and the repair of damaged tissues. Perivascular cells, including vascular smooth muscle cells, pericytes and other tissue specific populations residing around blood vessels, have been isolated from many organs and are known to participate to the in situ repair process and angiogenesis. Their potential has been harnessed for cell therapy of numerous pathologies; however, in this Review we will discuss the potential of perivascular cells in the development of tissue engineering solutions for healthcare...
March 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27834123/translational-applications-of-tissue-engineering-in-cardiovascular-medicine
#18
Arin Dogan, A Eser Elcin, Y Murat Elcin
Cardiovascular diseases are the leading cause of global deaths. The current paradigm in medicine seeks novel approaches for the treatment of progressive or end-stage diseases. The organ transplantation option is limited in availability, and unfortunately, a significant number of patients are lost while waiting for donor organs. Animal studies have shown that upon myocardial infarction, it is possible to stop adverse remodeling in its tracks and reverse with tissue engineering methods. Regaining the myocardium function and avoiding further deterioration towards heart failure can benefit millions of people with a significantly lesser burden on healthcare systems worldwide...
March 26, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27689781/poly-3-hydroxyoctanoate-a-promising-new-material-for-cardiac-tissue-engineering
#19
Andrea V Bagdadi, Maryam Safari, Prachi Dubey, Pooja Basnett, Panagiotis Sofokleous, Eleanor Humphrey, Ian Locke, Mohan Edirisinghe, Cesare Terracciano, Aldo R Boccaccini, Jonathan C Knowles, Sian E Harding, Ipsita Roy
Cardiac tissue engineering (CTE) is currently a prime focus of research due to an enormous clinical need. In this work, a novel functional material, Poly(3-hydroxyoctanoate), P(3HO), a medium chain length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mechanical properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topography, to allow efficient cell adhesion and proliferation, were fabricated...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27678265/promoting-cardiomyogenesis-of-hbmsc-with-a-forming-self-assembly-hbmsc-microtissues-ha-grgd-sf-pcl-cardiac-patch-is-mediated-by-the-synergistic-functions-of-ha-grgd
#20
Tze-Wen Chung, Hsin-Yu Lo, Tzung-Han Chou, Jan-Hou Chen, Shoei-Shen Wang
Bone marrow-derived mesenchymal stem cell microtissues (BMSCMT) enhanced cardiomyogenesis in vitro and cardiac repairs of myocardial infarcted hearts in vivo are documented. Producing human BMSCMT onto patches in vitro for cardiac tissue engineering has not been reported. For possibly producing human bone marrow-derived mesenchymal stem cell microtissues (hBMSCMT) on an elastic silk fibroin (SF)-poly(ε-caprolactone) (PCL) based patches is hereby designed. After an elastic SF-PCL (SP) patch is fabricated, hyaluronic acid (HA)/SF-PCL(HSP) and HA-GRGD/SF-PCL(HGSP) patches are fabricated by photochemically grafting HA and HA-GRGD onto SP surfaces...
March 2017: Macromolecular Bioscience
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