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Feng Zhang Broad

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https://www.readbyqxmd.com/read/28099762/human-transporters-pept1-2-facilitate-melatonin-transportation-into-mitochondria-of-cancer-cells-an-implication-of-the-therapeutic-potential
#1
Xiaokui Huo, Chao Wang, Zhenlong Yu, Yulin Peng, Shumei Wang, Shengnan Feng, Shouji Zhang, Xiangge Tian, Chengpeng Sun, Kexin Liu, Sa Deng, Xiaochi Ma
Melatonin is present in virtually all organisms from bacteria to mammals, and it exhibits a broad spectrum of biological functions, including synchronization of circadian rhythms and oncostatic activity. Several functions of melatonin are mediated by its membrane receptors but others are receptor-independent. For the latter, melatonin is required to penetrate membrane and enters intracellular compartments. However, the mechanism by which melatonin enters cells remains debatable. In the current study, it was identified that melatonin and its sulfation metabolites were the substrates of oligopeptide transporter (PEPT) 1/2 and organic anion transporter (OAT) 3, respectively...
January 18, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28060406/a-simple-protoplast-based-method-for-screening-potent-artificial-mirna-for-maximal-gene-silencing-in-arabidopsis
#2
Nannan Zhang, Dandan Zhang, Jian-Feng Li
The unpredictability of in planta silencing efficiency of artificial micro RNAs (amiRNAs) has greatly limited the application of the amiRNA technology in basic and applied plant research. This unit describes a simple and robust method called the epitope-tagged protein-based amiRNA (ETPamir) screen for identifying the most effective amiRNA for silencing a particular Arabidopsis gene. After selecting three to four amiRNA candidates designed by the WMD3 web server for the target gene, the silencing efficiencies of the amiRNA candidates can be compared by co-expressing individual amiRNAs with the target mRNA encoding an epitope-tagged target protein in Arabidopsis mesophyll protoplasts...
January 5, 2017: Current Protocols in Molecular Biology
https://www.readbyqxmd.com/read/28055187/synthesis-of-1-2-3-thiadizole-and-thiazole-based-strobilurins-as-potent-fungicide-candidates
#3
Lai Chen, Yu-Jie Zhu, Zhijin Fan, Xiao-Feng Guo, Zhi-Ming Zhang, Jing-Hua Xu, Ying-Qi Song, Yury Yurievich Morzherin, Nataliya P Belskaya, Vasiliy A Bakulev
Strobilurin fungicides play a crucial role in protecting plants against different pathogens and securing food supplies. A series of 1,2,3-thiadiazole and thiazole based strobilurins were rationally designed, synthesized, characterized and tested against various fungi. Introduction of 1,2,3-thiadiazole greatly improved the fungicidal activity of the target molecules. Compounds 8a, 8c, 8d and 10i exhibited a relative broad-spectrum of fungicidal activity. Compound 8a showed excellent activities against Gibberella zeae, Sclerotinia sclerotiorum and Rhizoctonia cerealis with the median effective concentration (EC50) of 2...
January 5, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28050902/erythrocyte-membrane-coated-upconversion-nanoparticles-with-minimal-protein-adsorption-for-enhanced-tumor-imaging
#4
Lang Rao, Qian-Fang Meng, Lin-Lin Bu, Bo Cai, Qinqin Huang, Zhi-Jun Sun, Wen-Feng Zhang, Andrew Li, Shi-Shang Guo, Wei Liu, Tza-Huei Wang, Xing-Zhong Zhao
Upconversion nanoparticles (UCNPs) with superior optical and chemical features have been broadly employed for in vivo cancer imaging. Generally, UCNPs are surface modified with ligands for cancer active targeting. However, nanoparticles in biological fluids are known to form a long-lived "protein corona", which covers the targeting ligands on nanoparticle surface and dramatically reduces the nanoparticle targeting capabilities. Here, for the first time, we demonstrated that by coating UCNPs with red blood cell (RBC) membranes, the resulting cell membrane-capped nanoparticles (RBC-UCNPs) adsorbed virtually no proteins when exposed to human plasma...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28027504/a-key-esterase-required-for-the-mineralization-of-quizalofop-p-ethyl-by-a-natural-consortium-of-rhodococcus-sp-jt-3-and-brevundimonas-sp-jt-9
#5
Hui Zhang, Mengya Li, Jie Li, Guangli Wang, Feng Li, Dayong Xu, Yuan Liu, Minghua Xiong
A natural consortium, named L1, of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9 was obtained from quizalofop-p-ethyl (QE) polluted soil. The consortium was able to use QE as a sole carbon source for growth and degraded 100mgL(-1) of QE in 60h. Strain JT-3 initiated the catabolism of QE to quizalofop acid (QA), which was used by strain JT-9 as carbon source for growth and to simultaneously feed strain JT-3. A novel esterase EstS-JT, which was responsible for the transformation of QE to QA and essential for the mineralization of QE by the consortium, was cloned from strain JT-3...
December 21, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28027492/design-of-antibacterial-biointerfaces-by-surface-modification-of-poly-%C3%AE%C2%B5-caprolactone-with-fusion-protein-containing-hydrophobin-and-pa-1
#6
Xiangxiang Wang, Jiwei Mao, Yiming Chen, Dongmin Song, Zhendong Gao, Xiuming Zhang, Yanling Bai, Per E J Saris, Hui Feng, Haijin Xu, Mingqiang Qiao
Class IIa bacteriocin pediocin PA-1 has broad-spectrum activity and is a well-characterized candidate food biopreservative. Here, a simple approach is designed to extend the application of pediocin PA-1 in improving the antibacterial activity of electrospun poly(caprolactone) (PCL) grafts through combining PA-1 with HGFI, which is a self-assembled protein with characteristics allowing the modulation of surface properties of other materials originated from Grifola frondosa. Saccharomyces cerevisiae was used as the host for expression of fusion protein PA-1-linker-HGFI (pH) and his-tag purification was used to purify recombinant protein pH...
December 16, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27999357/recent-methods-for-purification-and-structure-determination-of-oligonucleotides
#7
REVIEW
Qiulong Zhang, Huanhuan Lv, Lili Wang, Man Chen, Fangfei Li, Chao Liang, Yuanyuan Yu, Feng Jiang, Aiping Lu, Ge Zhang
Aptamers are single-stranded DNA or RNA oligonucleotides that can interact with target molecules through specific three-dimensional structures. The excellent features, such as high specificity and affinity for target proteins, small size, chemical stability, low immunogenicity, facile chemical synthesis, versatility in structural design and engineering, and accessible for site-specific modifications with functional moieties, make aptamers attractive molecules in the fields of clinical diagnostics and biopharmaceutical therapeutics...
December 18, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27998480/the-peroxidase-and-oxidase-like-activity-of-nico2o4-mesoporous-spheres-mechanistic-understanding-and-colorimetric-biosensing
#8
Li Su, Wenpei Dong, Chengke Wu, Yijun Gong, Yan Zhang, Ling Li, Guojiang Mao, Suling Feng
The synthesized NiCo2O4 mesoporous spheres (MS) displayed intrinsic peroxidase and oxidase-like activity were firstly reported. The catalytic mechanism of the oxidase-like activity of NiCo2O4 MS was analyzed in detail using the electron spin resonance (ESR) method. It is found that NiCo2O4 MS could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) but did not produce (1)O2 and ·OH. And the mechanism of the peroxidase-like activity of NiCo2O4 MS was also verified that the oxidation of TMB stemmed from not only ·OH but also (1)O2...
January 25, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27979206/identification-of-a-cyclodextrin-inclusion-complex-of-antimicrobial-peptide-cm4-and-its-antimicrobial-activity
#9
Jian-Feng Li, Jia-Xin Zhang, Zhi-Guo Wang, Ying-Jia Yao, Xu Han, You-Long Zhao, Jun-Ping Liu, Shuang-Quan Zhang
Antibacterial peptide CM4 (ABP-CM4) is a natural product isolated from the silkworm Bombyx mori. It is a small cationic peptide with broad-spectrum activities against harmful microorganisms and may be used as a novel food preservative. However, ABP-CM4 lacks tertiary structure in water-like solutions, which makes it more susceptible to proteases and labile when exposed to air. In this study, β-cyclodextrin (β-CD) was chosen to form an inclusion complex with ABP-CM4, which enhanced the physical and chemical properties of ABP-CM4 but did not decrease its antibacterial activity...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27978727/synthesis-of-o-alkenylated-2-arylbenzoxazoles-via-rh-catalyzed-oxidative-olefination-of-2-arylbenzoxazoles-scope-investigation-structural-features-and-mechanism-studies
#10
Quan Zhou, Jing-Fan Zhang, Hui Cao, Rui Zhong, Xiu-Feng Hou
2-Arylbenzazoles are promising molecules for potential applications in medicine and material areas. Efficient protocols for direct regioselective functionalization of 2-arylbenzoxazoles are in high demand. Herein, we disclose a general method for selective ortho-olefination of 2-arylbenzo[d]oxazoles with alkenes enabled by versatile Cp*Rh(III) in high yields. This protocol features broad functional group tolerance and high regioselectivity. Intermolecular competition studies and kinetic isotope effect experiments imply that the oxidative olefination process occurs via an electrophilic C-H activation pathway...
December 16, 2016: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/27977895/a-soluble-form-of-psgl-1-requires-nrf2-signaling-to-protect-liver-transplant-endothelial-cells-against-ischemia-reperfusion-injury
#11
Cheng Zhang, Yu Zhang, Yuanxing Liu, Yuan Liu, Shoichi Kageyama, Xiu-da Shen, Feng Gao, Shusen Zheng, Ronald W Busuttil, Gray D Shaw, Haofeng Ji, Jerzy W Kupiec-Weglinski
Liver endothelial cell (LEC) damage is essential in the pathogenesis of ischemia-reperfusion injury (IRI) in transplant recipients. We analyzed the mechanism of LEC resistance against IRI by using a novel recombinant soluble form of PSGL-1 (Tandem P-Selectin Glycoprotein Ligand-Immunoglobulin; TSGL-Ig) in a mouse model of hepatic cold preservation (4°C in UW for 20h) and syngeneic orthotopic liver transplantation (OLT). Unlike in controls, TSGL-Ig protected OLT against IR-stress, evidenced by depressed sALT levels, well-preserved hepatic architecture, and improved survival (42% vs...
December 15, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27934020/a-thieno-3-4-b-thiophene-based-non-fullerene-electron-acceptor-for-high-performance-bulk-heterojunction-organic-solar-cells
#12
Feng Liu, Zichun Zhou, Cheng Zhang, Thomas Vergote, Haijun Fan, Feng Liu, Xiaozhang Zhu
A thieno[3,4-b]thiophene-based electron acceptor, ATT-1, is designed and synthesized. ATT-1 exhibits a planar conjugated framework, broad absorption with a large absorption coefficient, and a slightly high LUMO energy level. Bulk-heterojunction (BHJ) solar cells based on PTB7-Th electron donor and ATT-1 electron acceptor delivered power conversion efficiencies of up to 10.07%, which is among the best performances reported for non-fullerene BHJ solar cells using PTB7-Th as the electron donor.
December 7, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27899283/a-high-throughput-microtiter-plate-assay-for-the-discovery-of-active-and-enantioselective-amino-alcohol-specific-transaminases
#13
Jian-Dong Zhang, Hua-Lei Wu, Tong Meng, Chao-Feng Zhang, Xiao-Jun Fan, Hong-Hong Chang, Wen-Long Wei
Chiral vicinal amino alcohols are important chiral building blocks and intermediates in the pharmaceutical industry. The transaminase (TAm) catalyzed kinetic resolution of racemic amino alcohols provides a straightforward approach to access these important compounds. This study describes the development of a novel microtiter plate assay to screen vicinal amino alcohol-specific TAms using a tetrazolium red-based colorimetric assay to monitor the rate of α-hydroxy ketone formation at 510 nm. This approach is the first to determine the Michaelis-Menten parameters for a recombinant TAm (PpbauA) from Pseudomonas putida NBRC14164...
February 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/27890732/programmed-near-infrared-light-responsive-drug-delivery-system-for-combined-magnetic-tumor-targeting-magnetic-resonance-imaging-and-chemo-phototherapy
#14
Qianhua Feng, Yuanyuan Zhang, Wanxia Zhang, Yongwei Hao, Yongchao Wang, Hongling Zhang, Lin Hou, Zhenzhong Zhang
: In this study, an intelligent drug delivery system was developed by capping doxorubicin (DOX)-loaded hollow mesoporous CuS nanoparticles (HMCuS NPs) with superparamagnetic iron oxide nanoparticles (IONPs). Under near infrared (NIR) light irradiation, the versatile HMCuS NPs could exploit the merits of both photothermal therapy (PTT) and photodynamic therapy (PDT) simultaneously. Herein, the multifunctional IONPs as gatekeeper with the enhanced capping efficiency were supposed to realize "zero premature release" and minimize the adverse side effects during the drug delivery in vivo...
November 24, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27874082/ultra-broadband-reflective-metamaterial-with-rcs-reduction-based-on-polarization-convertor-information-entropy-theory-and-genetic-optimization-algorithm
#15
Si Jia Li, Xiang Yu Cao, Li Ming Xu, Long Jian Zhou, Huan Huan Yang, Jiang Feng Han, Zhao Zhang, Di Zhang, Xiao Liu, Chen Zhang, Yue Jun Zheng, Yi Zhao
We proposed an ultra-broadband reflective metamaterial with controlling the scattering electromagnetic fields based on a polarization convertor. The unit cell of the polarization convertor was composed of a three layers substrate with double metallic split-rings structure and a metal ground plane. The proposed polarization convertor and that with rotation angle of 90 deg had been employed as the "0" and "1" elements to design the digital reflective metamaterial. The numbers of the "0" and "1" elements were chosen based on the information entropy theory...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27866260/isolation-and-characterization-of-a-novel-cadmium-regulated-yellow-stripe-like-transporter-snysl3-in-solanum-nigrum
#16
Shanshan Feng, Jinjuan Tan, Yuxiu Zhang, Shuang Liang, Shuqin Xiang, Hong Wang, Tuanyao Chai
SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake and long-range transport in model plants. In this study, we isolated and characterized a novel member of the YSL gene family, SnYSL3, from the cadmium hyperaccumulator Solanum nigrum. SnYSL3 was constitutively expressed and encodes a plasma membrane-localized protein...
November 19, 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27862459/enantioselective-synthesis-of-n-h-free-1-5-benzothiazepines
#17
Guojin Wang, Yu Tang, Yu Zhang, Xiaohua Liu, Lili Lin, Xiaoming Feng
An enantioselective sulfa-Michael-cyclization reaction was developed for the synthesis of 1,5-benzothiazepines with versatile pharmacological activities. The reaction between 2-aminothiophenol and α,β-unsaturated pyrazoleamides gave direct access to N-H-free 1,5-benzothiazepines in the presence of a chiral N,N'-dioxide/Yb(OTf)3 complex. Excellent enantioselectivities (up to 96 % ee) and high yields (up to 99 %) were obtained for a broad range of substrates under mild reaction conditions. This method provided a facile approach to the antidepressant drug (R)-(-)-Thiazesim...
November 15, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27852038/transcriptomic-metabolomic-reprogramming-in-egfr-mutant-nsclc-early-adaptive-drug-escape-linking-tgf%C3%AE-2-bioenergetics-mitochondrial-priming
#18
Praveena S Thiagarajan, Xiaoliang Wu, Wei Zhang, Ivy Shi, Rakesh Bagai, Patrick Leahy, Yan Feng, Martina Veigl, Daniel Lindner, David Danielpour, Lihong Yin, Rafael Rosell, Trever G Bivona, Zhenfeng Zhang, Patrick C Ma
The impact of EGFR-mutant NSCLC precision therapy is limited by acquired resistance despite initial excellent response. Classic studies of EGFR-mutant clinical resistance to precision therapy were based on tumor rebiopsies late during clinical tumor progression on therapy. Here, we characterized a novel non-mutational early adaptive drug-escape in EGFR-mutant lung tumor cells only days after therapy initiation, that is MET-independent. The drug-escape cell states were analyzed by integrated transcriptomic and metabolomics profiling uncovering a central role for autocrine TGFβ2 in mediating cellular plasticity through profound cellular adaptive Omics reprogramming, with common mechanistic link to prosurvival mitochondrial priming...
November 11, 2016: Oncotarget
https://www.readbyqxmd.com/read/27825886/upconversion-nanoparticles-based-fret-aptasensor-for-rapid-and-ultrasenstive-bacteria-detection
#19
Birui Jin, Shurui Wang, Min Lin, Ying Jin, Shujing Zhang, Xingye Cui, Yan Gong, Ang Li, Feng Xu, Tian Jian Lu
Pathogenic bacteria cause serious harm to human health, which calls for the development of advanced detection methods. Herein, we developed a novel detection platform based on fluorescence resonance energy transfer (FRET) for rapid, ultrasensitive and specific bacteria detection, where gold nanoparticles (AuNPs, acceptor) were conjugated with aptamers while upconversion nanoparticles (UCNPs, donor) were functionalized with corresponding complementary DNA (cDNA). The spectral overlap between UCNPs fluorescence emission and AuNPs absorption enables the occurrence of FRET when hybridizing the targeted aptamer and cDNA, causing upconversion fluorescence quenching...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27816473/characterization-of-soluble-n-ethylmaleimide-sensitive-factor-attachment-protein-receptor-gene-stx18-variations-for-possible-roles-in-congenital-heart-diseases
#20
Xia Li, Shuai Shi, Fei-Feng Li, Rui Cheng, Ying Han, Li-Wei Diao, Qiong Zhang, Ji-Xin Zhi, Shu-Lin Liu
Congenital heart disease (CHD) is among the most prevalent and complex congenital anatomic malformations in newborns. Interactions of cardiac progenitor with a broad range of cellular regulatory factors play key roles in the formation of mammalian heart and pathogenesis of CHD. STX18 is a soluble N-ethylmaleimide-sensitive factor attachment protein receptor, which is involved in numeral cellular activities such as organelle assembly and the cell cycle. The aim of this work was to find evidence on whether STX18 variations might be associated with CHD in Chinese Han populations...
January 20, 2017: Gene
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