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https://www.readbyqxmd.com/read/28920176/isolation-and-identification-of-antifungal-peptides-from-bacillus-amyloliquefaciens-w10
#1
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography...
September 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28903899/the-application-of-next-generation-sequencing-techniques-in-studying-transcriptional-regulation-in-embryonic-stem-cells
#2
Ya-Jun Liu, Feng Zhang, Hong-de Liu, Xiao Sun
The mechanism of transcriptional regulation has been the focus of many studies in the post-genomic era. The development of sequencing-based technologies for chromatin profiling enables current researchers to experimentally measure chromatin properties. Moreover, many studies aim at annotating the state of the chromatin into broad categories based on observed chromatin features and/or DNA sequences, then associating the resultant distal regulatory regions with the correct target genes based on DNA sequences, and predicting the dependence of epigenetic features on genetic variation...
August 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28868560/nickel-catalyzed-direct-c-h-trifluoroethylation-of-heteroarenes-with-trifluoroethyl-iodide
#3
Sheng-Yi Yan, Zhuo-Zhuo Zhang, Bing-Feng Shi
A highly selective nickel-catalyzed C-H trifluoroethylation of heteroarenes was developed with the assistance of a monodentate directing group. This protocol provides efficient access to various trifluoroethyl-substituted heteroarenes, including indoles, pyrroles, furans, and thiophenes, with commercially available CF3CH2I as an alkylation reagent. This robust catalytic procedure is scalable and tolerates a broad range of functional groups. Moreover, multifluoroalkylation of indoles is also viable.
September 14, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28867588/light-deprivation-produces-distinct-morphological-orchestrations-on-rgcs-and-cortical-cells-in-a-depressive-like-yfp-h-mouse-model
#4
Chanyi Lu, Qi-Qin Li, Yao-Yao Li, Hua-Zhen Lin, Jia Qu, Yun Wang, Yun-Feng Zhang
Neural physiological functions and synaptic changes underlying the pathogenesis of depression have obtained great achievements. However, neuronal morphological changes under a depressive state have not been well understood yet. Here a depressive-like YFP-H transgenic mouse model was produced by light deprivation (LD), and morphological changes of retinal ganglion cells (RGCs) and primary visual and auditory cortical layer 5 pyramidal cells (L5PCs) were investigated. Three distinct RGC subtypes were identified based on soma- and dendritic field (DF) size...
September 1, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28860505/endolysin-lysef-p10-shows-potential-as-an-alternative-treatment-strategy-for-multidrug-resistant-enterococcus-faecalis-infections
#5
Mengjun Cheng, Yufeng Zhang, Xinwei Li, Jiaming Liang, Liyuan Hu, Pengjuan Gong, Lei Zhang, Ruopeng Cai, Hao Zhang, Jinli Ge, Yalu Ji, Zhimin Guo, Xin Feng, Changjiang Sun, Yongjun Yang, Liancheng Lei, Wenyu Han, Jingmin Gu
Phage-derived lysins can hydrolyse bacterial cell walls and show great potential for combating Gram-positive pathogens. In this study, the potential of LysEF-P10, a new lysin derived from a isolated Enterococcus faecalis phage EF-P10, as an alternative treatment for multidrug-resistant E. faecalis infections, was studied. LysEF-P10 shares only 61% amino acid identity with its closest homologues. Four proteins were expressed: LysEF-P10, the cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domain (LysEF-P10C), the putative binding domain (LysEF-P10B), and a fusion recombination protein (LysEF-P10B-green fluorescent protein)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28858725/focus-on-the-therapeutic-efficacy-of-3bnc117-against-hiv-1-in-vitro-studies-in-vivo-studies-clinical-trials-and-challenges
#6
REVIEW
Zhi-Jun Liu, Jing Bai, Feng-Li Liu, Xiang-Yang Zhang, Jing-Zhang Wang
3BNC117, which was discovered in 2011, is a broadly neutralizing antibody (bNAb) and specifically neutralizes the human immunodeficiency virus type-1 (HIV-1) by targeting the CD4-binding site. This is the first comprehensive review that focuses on the role of 3BNC117 in the prevention of HIV-1 and acquired immune deficiency syndrome (AIDS). Briefly, 3BNC117 neutralizes many HIV/SHIV strains in vitro, blocks HIV-1 acquisition in animal models via a pre-exposure prophylaxis, alleviates HIV-1-associated viremia via a post-exposure therapeutic effect, prevents the establishment of latent HIV-1 reservoirs, and induces both humoral and cellular anti-HIV immune responses in vivo...
August 28, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28837314/thieno-3-2-b-pyrrolo-fused-pentacyclic-benzotriazole-based-acceptor-for-efficient-organic-photovoltaics
#7
Liuliu Feng, Jun Yuan, Zhenzhen Zhang, Hongjian Peng, Zhi-Guo Zhang, Shutao Xu, Ye Liu, Yongfang Li, Yingping Zou
A novel nonfullerene small molecular acceptor (BZIC) based on a ladder-type thieno[3,2-b]pyrrolo-fused pentacyclic benzotriazole core (dithieno[3,2-b]pyrrolobenzotriazole, BZTP) and end-capped with 1,1-dicyanomethylene-3-indanone (INCN) has been first reported in this work. Through introducing multifused benzotriazole and INCN, BZIC could maintain a high-lying lowest unoccupied molecular orbital (LUMO) energy level of -3.88 eV. Moreover, BZIC shows a low optical bandgap of 1.45 eV with broad and efficient absorption band from 600 to 850 nm due to increased π-π interactions by the covalently locking thiophene and benzotriazole units...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28837166/chlorodifluoromethane-triggered-formation-of-difluoromethylated-arenes-catalysed-by-palladium
#8
Zhang Feng, Qiao-Qiao Min, Xia-Ping Fu, Lun An, Xingang Zhang
Difluoromethylated aromatic compounds are of increasing importance in pharmaceuticals, agrochemicals and materials. Chlorodifluoromethane (ClCF2H), an inexpensive, abundant and widely used industrial raw material, represents the ideal and most straightforward difluoromethylating reagent, but introduction of the difluoromethyl group (CF2H) from ClCF2H into aromatics has not been reported. Here, we describe a direct palladium-catalysed difluoromethylation method for coupling ClCF2H with arylboronic acids and esters to generate difluoromethylated arenes with high efficiency...
September 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28830557/membrane-anchored-ccl20-augments-hiv-env-specific-mucosal-immune-responses
#9
Xianliang Sun, Han Zhang, Shuiling Xu, Lili Shi, Jingjian Dong, Dandan Gao, Yan Chen, Hao Feng
BACKGROUND: Induction of broad immune responses at mucosal site remains a primary goal for most vaccines against mucosal pathogens. Abundance of evidence indicates that the co-delivery of mucosal adjuvants, including cytokines, is necessary to induce effective mucosal immunity. In the present study, we set out to evaluate the role of a chemokine, CCL20, as an effective mucosal adjuvant for HIV vaccine. METHODS: To evaluate the role of CCL20 as a potent adjuvant for HIV vaccine, we examined its effects on antigen-specific antibody responses, level of antibody-secreting cells, cytokine production and intestinal homing of plasma cells in vaccine immunized mice...
August 23, 2017: Virology Journal
https://www.readbyqxmd.com/read/28827346/structures-of-phlebovirus-glycoprotein-gn-and-identification-of-a-neutralizing-antibody-epitope
#10
Yan Wu, Yaohua Zhu, Feng Gao, Yongjun Jiao, Babayemi O Oladejo, Yan Chai, Yuhai Bi, Shan Lu, Mengqiu Dong, Chang Zhang, Guangmei Huang, Gary Wong, Na Li, Yanfang Zhang, Yan Li, Wen-Hai Feng, Yi Shi, Mifang Liang, Rongguang Zhang, Jianxun Qi, George F Gao
Severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley fever virus (RVFV) are two arthropod-borne phleboviruses in the Bunyaviridae family, which cause severe illness in humans and animals. Glycoprotein N (Gn) is one of the envelope proteins on the virus surface and is a major antigenic component. Despite its importance for virus entry and fusion, the molecular features of the phleboviruse Gn were unknown. Here, we present the crystal structures of the Gn head domain from both SFTSV and RVFV, which display a similar compact triangular shape overall, while the three subdomains (domains I, II, and III) making up the Gn head display different arrangements...
August 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28826211/high-strength-and-hydrophilic-chitosan-microspheres-for-the-selective-enrichment-of-n-glycopeptides
#11
Xiao-Mei He, Xi-Chao Liang, Xi Chen, Bi-Feng Yuan, Ping Zhou, Li-Na Zhang, Yu-Qi Feng
Protein glycosylation is an important post-translational modification that plays a crucial role in many biological processes. Because of the low abundance of glycoproteins and high complexity of clinical samples, the development of methods to selectively capture glycoproteins/glycopeptides is crucial to glycoproteomics study. In this work, a kind of highly cross-linked chitosan microspheres (CSMs) was prepared using epichlorhydrine as a cross-linker from chitosan solution in an alkaline/urea aqueous system...
August 31, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28814642/an-indel-in-the-promoter-of-al-activated-malate-transporter-9-selected-during-tomato-domestication-determines-fruit-malate-contents-and-aluminum-tolerance
#12
Jie Ye, Xin Wang, Ti Xu Hu, Feng Xia Zhang, Bing Wang, Chang Xin Li, Tian Xia Yang, Han Xia Li, Yong En Lu, James J Giovannoni, Yuyang Zhang, Zhibiao Ye
Deciphering the mechanism of malate accumulation in plants would contribute to a greater understanding of plant chemistry, which has implications for improving flavor quality in crop species and enhancing human health benefits. However, the regulation of malate metabolism is poorly understood in crops such as tomato (Solanum lycopersicum). Here, we integrated a metabolite-based genome-wide association study (mGWAS) with linkage mapping and gene functional studies to characterize the genetics of malate accumulation in a global collection of tomato accessions with broad genetic diversity...
August 16, 2017: Plant Cell
https://www.readbyqxmd.com/read/28804606/a-multiple-antigen-detection-assay-for-tuberculosis-diagnosis-based-on-broadly-reactive-polyclonal-antibodies
#13
Zhenhua Dai, Zhiqiang Liu, Bingshui Xiu, Xiqin Yang, Ping Zhao, Xuhui Zhang, Cuimi Duan, Haiping Que, Heqiu Zhang, Xiaoyan Feng
OBJECTIVES: Detection of circulating Mycobacterium tuberculosis (M. tuberculosis) antigens is promising in Tuberculosis (TB) diagnosis. However, not a single antigen marker has been found to be widely expressed in all TB patients. This study is aimed to prepare broadly reactive polyclonal antibodies targeting multiple antigen markers (multi-target antibodies) and evaluate their efficacies in TB diagnosis. MATERIALS AND METHODS: A fusion gene consisting of 38kD, ESAT6, and CFP10 was constructed and overexpressed...
April 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28802244/detoxification-of-diphenyl-ether-herbicide-lactofen-by-bacillus-sp-za-and-enantioselective-characteristics-of-an-esterase-gene-lace
#14
Jing Zhang, Luyao Lu, Feng Chen, Lingling Chen, Jingang Yin, Xing Huang
A bacterial strain Za capable of degrading diphenyl ether herbicide lactofen was isolated and identified as Bacillus sp. This strain could degrade 94.8% of 50mgL(-1) lactofen after 4days of inoculation in flasks. It was revealed that lactofen was initially hydrolyzed to desethyl lactofen, which was further transformed to acifluorfen, followed by the reduction of the nitro group to yield aminoacifluorfen. The phytotoxicity of the transformed product aminoacifluorfen to maize was decreased significantly compared with the lactofen...
August 3, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28801583/effect-of-x-ray-irradiation-on-hepatocarcinoma-cells-and-erythrocytes-in-salvaged-blood
#15
Feng-Jiang Zhang, Jin-Ting Yang, Li-Hui Tang, Wen-Na Wang, Kai Sun, Yue Ming, Kanhar Ghulam Muhammad, Yin-Fei Zheng, Min Yan
The broad clinical acceptance of intraoperative blood salvage and its applications in cancer surgery remain controversial. Until now, a method that can safely eliminate cancer cells while preserving erythrocytes does not exist. Here, we investigated whether X-ray generated from linear accelerator irradiation at a certain dose can kill hepatocarcinoma cells while preserving erythrocytes. HepG2, SK-Hep1 or Huh7 cells were mixed into the aliquots of erythrocytes obtained from healthy volunteers. After the mixed cells were exposed to 30 Gy and 50 Gy X-rays irradiation, the viability, clonogenicity, DNA synthesis and tumorigenicity of the tumor cells were determined by the MTT assay, plate colony formation, 5-ethynyl-2'-deoxyuridine incorporation, and subcutaneous xenograft implantation into immunocompromised mice...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28755294/vdpks1-is-required-for-melanin-formation-and-virulence-in-a-cotton-wilt-pathogen-verticillium-dahliae
#16
Ting Zhang, Bosen Zhang, Chenlei Hua, Pei Meng, Sheng Wang, Zhirong Chen, Yejuan Du, Feng Gao, Jiafeng Huang
Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt disease in a broad range of hosts. This pathogen survives for many years in soil in the form of melanized microsclerotia. To investigate the melanin synthesis in V. dahliae, we identified a polyketide synthase gene in V. dahliae, namely VdPKS1. PKS1 is known to involve in the dihydroxynaphthalene melanin synthesis pathway in many fungi. We found that VdPKS1 was required for melanin formation but not for microsclerotial production in V...
July 26, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28752765/gold-embedded-hollow-silica-nanogolf-balls-for-imaging-and-photothermal-therapy
#17
Woraphong Janetanakit, Liping Wang, Karla Santacruz-Gomez, Preston B Landon, Paul L Sud, Nirav Patel, Grace Jang, Malvika Jain, Alice Yepremyan, Sami A Kazmi, Deependra K Ban, Feng Zhang, Ratnesh Lal
Hybrid nanocarriers with multifunctional properties have wide therapeutic and diagnostic applications. We have constructed hollow silica nanogolf balls (HGBs) and gold-embedded hollow silica nanogolf balls (Au@SiO2 HGBs) using the layer-by-layer approach on a symmetric polystyrene (PS) Janus template; the template consists of smaller PS spheres attached to an oppositely charged large PS core. ζ Potential measurement supports the electric force-based template-assisted synthesis mechanism. Electron microscopy, UV-vis, and near-infrared (NIR) spectroscopy show that HGBs or Au@SiO2 HGBs are composed of a porous silica shell with an optional dense layer of gold nanoparticles embedded in the silica shell...
August 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28706472/relevant-feature-integration-and-extraction-for-single-trial-motor-imagery-classification
#18
Lili Li, Guanghua Xu, Feng Zhang, Jun Xie, Min Li
Brain computer interfaces provide a novel channel for the communication between brain and output devices. The effectiveness of the brain computer interface is based on the classification accuracy of single trial brain signals. The common spatial pattern (CSP) algorithm is believed to be an effective algorithm for the classification of single trial brain signals. As the amplitude feature for spatial projection applied by this algorithm is based on a broad frequency bandpass filter (mainly 5-30 Hz) in which the frequency band is often selected by experience, the CSP is sensitive to noise and the influence of other irrelevant information in the selected broad frequency band...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28704723/highly-sensitive-determination-of-fatty-acid-esters-of-hydroxyl-fatty-acids-by-liquid-chromatography-mass-spectrometry
#19
Quan-Fei Zhu, Jing-Wen Yan, Yang Gao, Jing-Wei Zhang, Bi-Feng Yuan, Yu-Qi Feng
Recently, a new class of endogenous lipids, branched fatty acid esters of hydroxy fatty acids (FAHFAs), was discovered with anti-diabetic and anti-inflammatory effects in mammals. FAHFAs attracted increasing attention because of their critical physiological function. However, accurate quantitation of FAHFAs is still a challenge due to their high structure similarity and low abundance in biological samples. Herein, we developed a highly sensitive method for the determination of 16 FAHFAs (PAHSAs, OAHSAs, SAHSAs and POHSAs) in biological samples by coupling strong anion exchange solid phase extraction (SAX-SPE) with chemical labeling assisted ultra-high performance liquid chromatography/mass spectrometry (SAX-SPE-CL-UHPLC/MS)...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28685770/existing-drugs-as-broad-spectrum-and-potent-inhibitors-for-zika-virus-by-targeting-ns2b-ns3-interaction
#20
Zhong Li, Matthew Brecher, Yong-Qiang Deng, Jing Zhang, Srilatha Sakamuru, Binbin Liu, Ruili Huang, Cheri A Koetzner, Christina A Allen, Susan A Jones, Haiying Chen, Na-Na Zhang, Min Tian, Fengshan Gao, Qishan Lin, Nilesh Banavali, Jia Zhou, Nathan Boles, Menghang Xia, Laura D Kramer, Cheng-Feng Qin, Hongmin Li
Recent outbreaks of Zika virus (ZIKV) highlight an urgent need for therapeutics. The protease complex NS2B-NS3 plays essential roles during flaviviral polyprotein processing, and thus represents an attractive drug target. Here, we developed a split luciferase complementation-based high-throughput screening assay to identify orthosteric inhibitors that directly target flavivirus NS2B-NS3 interactions. By screening a total of 2 816 approved and investigational drugs, we identified three potent candidates, temoporfin, niclosamide, and nitazoxanide, as flavivirus NS2B-NS3 interaction inhibitors with nanomolar potencies...
August 2017: Cell Research
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