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Aikaterini D Lianou, Vasileios Ragos, Panagiotis P Fotiades, Evangelos Tsiambas, Anna Batistatou, Ioannis Kastanioudakis
Human papillomavirus (HPV)-mediated cervical carcinogenesis represents a well analyzed model of viral implication in epithelial malignant transformation. Mechanisms of high risk (HR) HPV-related infection seem to demonstrate a similar action regarding its implication in head and neck (HN) carcinomas, predominantly in squamous cell carcinoma (SCC) histological type. The prevalence of HR HPV subtypes - mainly HPV16 - is characterized by a broad geographic heterogeneity. Furthermore, HPV-associated HNSCCs demonstrate differences regarding sexual, molecular, epidemiological, and prognostic features compared to alcohol and tobacco dependent ones...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Lucine Vuitton, Elise Jacquin, Anne-Laure Parmentier, Elise Crochet, Francine Fein, Anne-Claire Dupont-Gossart, Laurianne Plastaras, Charles-Henri Bretagne, Frédéric Mauny, Stéphane Koch, Jean-Luc Prétet, Christiane Mougin, Séverine Valmary-Degano
BACKGROUND & AIMS: The increasing incidence of anal canal carcinomas requires better knowledge on anal human papillomavirus (HPV) infection. We aimed to assess anal canal HPV infection prevalence and risk factors among patients seen at a gastroenterology department in France. METHODS: We analyzed anal tissue samples collected from 469 consecutive patients (median age 54 years, 52% women), including 112 who received immunosuppressant therapies and 101 with inflammatory bowel disease (70 with Crohn's disease), who underwent colonoscopy examinations from April 1, 2012 to April 30, 2015...
March 15, 2018: Clinical Gastroenterology and Hepatology
I Nikas, A Hapfelmeier, M Mollenhauer, D Angermeier, M Bettstetter, R Götz, M Schmidmayr, V Seifert-Klauss, A Muckenhuber, U Schenck, Gregor Weirich
Pathogenic microbes may colonize the female genital tract via sexual transmission and cause health issues like inflammation or malignancy, summarized as sexually transmitted disease (STD). A major representative of such pathogens is Trichomonas vaginalis (T.v.), whose role in the etiology of cervical cancer remains elusive. Traditional morphologic screening of cervical smears is able to detect T.v., although its identification may be complicated by look-alikes such as degenerated granulocytes and basal cells...
March 17, 2018: Parasitology Research
Azucena Muñoz-Ramírez, Aracely López-Monteon, Angel Ramos-Ligonio, Enrique Méndez-Bolaina, Mario R B Guapillo-Vargas
Female sex workers (FSWs) have been considered a key population for sexually transmitted infections (STIs); therefore, they are periodically screened as a requirement to obtain a work card. However, there is insufficient epidemiological data on STIs among FSWs in Mexico. The detection of Trichomonas vaginalis is limited to microscopic studies and the molecular screening of Human papillomavirus (HPV) is only done to women 35 years of age and older. The objective of this study was to determine the prevalence of T...
March 13, 2018: Revista Argentina de Microbiología
Mohammad Hadi Karbalaie Niya, Hossein Keyvani, Fahimeh Safarnezhad Tameshkel, Mostafa Salehi-Vaziri, Sedigheh Teaghinezhad-S, Farah Bokharaei Salim, Seyed Hamid Reza Monavari, Davod Javanmard
Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16-positive specimens were used for further analysis...
March 13, 2018: Translational Oncology
Aixa M Pérez-Caraballo, Erick Suarez, Elizabeth R Unger, Joel M Palefsky, Gitika Panicker, Ana Patricia Ortiz
OBJECTIVE: It is unknown if human papillomavirus (HPV) serum antibody responses vary by anatomic site of infection. We aimed to assess the seroprevalence for HPV 6, 11, 16 and 18 in association with HPV DNA detection in different anatomic sites among women. METHODS: This cross sectional population-based study analyzed data from 524 women aged 16-64 years living in the San Juan metropolitan area of Puerto Rico (PR). Questionnaires were used to assess demographic and lifestyle variables, while anogenital and blood samples were collected for HPV analysis...
March 2018: Puerto Rico Health Sciences Journal
Gennaro Ilardi, Daniela Russo, Silvia Varricchio, Giovanni Salzano, Giovanni Dell'Aversana Orabona, Virginia Napolitano, Rosa Maria Di Crescenzo, Alessandra Borzillo, Francesco Martino, Francesco Merolla, Massimo Mascolo, Stefania Staibano
Human Papilloma Virus (HPV) can play a causative role in the development of sinonasal tract malignancies. In fact, HPV may be the most significant causative agent implicated in sinonasal tumorigenesis and is implicated in as many as 21% of sinonasal carcinomas. To date, there are no definitive, reliable and cost-effective, diagnostic tests approved by the FDA for the unequivocal determination of HPV status in head and neck cancers. We followed an exhaustive algorithm to correctly test HPV infection, including a sequential approach with p16INK4a IHC, viral DNA genotyping and in situ hybridization for E6/E7 mRNA...
March 16, 2018: International Journal of Molecular Sciences
Beibei Zhang, Qiao Wang, Xinhui Xu, Qiang Xia, Feifei Long, Weiwei Li, Yingchun Shui, Xinyi Xia, Jinke Wang
This study develops a new method for detecting target DNA based on Cas9 nuclease, which was named as CARP, representing CRISPR- or Cas9/sgRNAs-associated reverse PCR. This technique detects target DNA in three steps: (1) cleaving the detected DNA sample with Cas9 in complex with a pair of sgRNAs specific to target DNA; (2) ligating the cleaved DNA with DNA ligase; (3) amplifying target DNA with PCR. In the ligation step, the Cas9-cut target DNA was ligated into intramolecular circular or intermolecular concatenated linear DNA...
March 15, 2018: Analytical and Bioanalytical Chemistry
Suhong Xiao, Ying-Sheng Tang, Praveen Kusumanchi, Sally P Stabler, Ying Zhang, Asok C Antony
Background: Epidemiologic and in vitro studies suggest independent linkages between poor folate and/or vitamin B-12 nutrition, genomic human papillomavirus (HPV) type 16 viral integration, and cancer. However, there is no direct evidence in vivo to support the causative role of poor folate nutrition in HPV16 integration into the cellular genome. Objective: We tested the hypothesis that folate deficiency enables the integration of HPV16 into the genome of HPV16-harboring keratinocytes, and could thereby influence earlier transformation of these cells to cancer in an animal model...
March 1, 2018: Journal of Nutrition
Pablo Romero-Morelos, Cindy Bandala, Julián Jiménez-Tenorio, Mariana Valdespino-Zavala, Miriam Rodríguez-Esquivel, Reyna Anaid Gama-Ríos, Artfy Bandera, Mónica Mendoza-Rodríguez, Keiko Taniguchi, Daniel Marrero-Rodríguez, Ricardo López-Romero, Eva Ramón-Gallegos, Mauricio Salcedo
BACKGROUND AND OBJECTIVE: Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and Gardnerella vaginalis has been suggested as potential risk factor for cervical lesions and cervical cancer. MATERIAL AND METHODS: DNA from 177 cervical scraping samples was studied: 104 belonged to women without cytological or colposcopic alterations and 73 samples from precursor lesions with previous human papillomavirus (HPV) infection history...
March 12, 2018: Medicina Clínica
Yimin Ge, Paul Christensen, Eric Luna, Donna Armylagos, Jiaqiong Xu, Mary R Schwartz, Dina R Mody
OBJECTIVE: Human papillomavirus (HPV) tests and genotyping (GT) have been used in clinical risk assessment. The purpose of this study was to analyze the performance of 2 common HPV testing platforms in risk evaluation for high-grade cervical lesions. MATERIALS AND METHODS: Between January 1, 2015, and December 31, 2016, a total of 4,562 Pap tests with follow-up biopsies in our laboratory database were analyzed along with HPV tests performed on Cobas (CHPV, n = 3,959) or Aptima (AHPV, n = 603) platforms...
March 14, 2018: Journal of Lower Genital Tract Disease
Sari L Reisner, Madeline B Deutsch, Sarah M Peitzmeier, Jaclyn M White Hughto, Timothy P Cavanaugh, Dana J Pardee, Sarah A McLean, Lori A Panther, Marcy Gelman, Matthew J Mimiaga, Jennifer E Potter
BACKGROUND: High-risk human papillomavirus (hrHPV) causes virtually all cervical cancers. Trans masculine (TM) people (those assigned female at birth who identify with a gender other than female) have low uptake of conventional cervical cancer screening. Self-collected hrHPV DNA testing has high levels of acceptability among cisgender (non-transgender) females and may support increased cervical cancer screening uptake in TM individuals. OBJECTIVE: To assess the test performance and acceptability of self-collected vaginal specimens in comparison to provider-collected cervical swabs for hrHPV DNA detection in TM individuals ages 21-64 years...
2018: PloS One
Murali Aarthy, Deepak Kumar, Rajanish Giri, Sanjeev Kumar Singh
Human papillomavirus (HPV) has been the primary causative agent of cervical cancer, the most threatening cancer affecting millions of women worldwide. HPV, a small non enveloped DNA virus of high and low risk types contain intrinsically disordered region and it also plays significant role in the development of cervical cancer. HPV E7 contains an ordered Zinc finger motif that binds to pRB and alters its function. It utilizes both disordered N-terminal and structured C-terminal regions for cellular transformation...
March 10, 2018: Gene
M Salakova, E Koslabova, M Grega, J Smahelova, J Klozar, O Vencalek, R Tachezy
Human papillomavirus (HPV) infection is associated with tonsillar cancer (TC) whose incidence in humans is increasing. Tonsillar tumours are not ordinarily preceded by clinically apparent precancerous lesions, and no markers of the early stage disease are available. Therefore, we evaluated the presence of an active HPV infection also in tumour-free tonsillar tissue. Formalin-fixed paraffin-embedded (FFPE) tonsillar specimens from 114 patients with TC and 114 age and gender matched controls were screened for the presence of HPV DNA, expression of HR-HPV E6 mRNA, and p16 overexpression...
2018: Neoplasma
Hanna Artemchuk, Tina Triglav, Anja Oštrbenk, Mario Poljak, Joakim Dillner, Helena Faust
Background: The antibody response against HPV-16 and -18 is well known, but many genital HPV types are oncogenic. We assessed the correlation between HPV DNA and antibodies to eleven HPV types. Methods: 2,024 women attending the organized national cervical cancer screening program in Slovenia were tested for cervical high-risk HPV DNA (HPV-16,-18,-31,-33,-35,-39,-45,-52,-56,-58,-59,-68) and serum anti-HPV antibodies. 1,848 of these women were tested with the same methods three years earlier...
February 26, 2018: Journal of Infectious Diseases
Sarah Cotton, Robert E Brown, Elizabeth K Nugent, Sonia C Robazetti, Pamela D Berens, Judith A Smith
OBJECTIVE: To assess the consistency between human papillomavirus (HPV) mRNA testing in women with a history of previous HPV infections diagnosed by HPV DNA assay and the potential effects on follow-up HPV screening. METHODS: This was a quality improvement study that used data from a pathology laboratory software database reviewed from November 2014 to June 2016 to identify female patients aged 30 years or older with greater than one HPV-positive result, including one or more HPV mRNA assay results and one or more documented HPV DNA assay results for comparison...
March 8, 2018: Obstetrics and Gynecology
Yasmine Ghantous, Sharon Akrish, Yoav Leiser, Imad Abu El-Naaj
BACKGROUND: Several types of human papillomavirus (HPV) have been found to be associated with oral squamous cell carcinoma (OSCC). Still, the significance of HPV infection and its relationship to patient prognosis remains an important matter of debate. OBJECTIVES: To investigate the incidence of HPV infection in OSCC patients in northern Israel populations to determine its role in the etiology and prognosis of OSCC. METHODS: OSCC tissues were gathered from the pathology departments at Rambam and Padeh medical centers in northern Israel...
March 2018: Israel Medical Association Journal: IMAJ
Xiumin Zhao, Qiongyan Wu, Xinyu Wang, Yunfeng Fu, Xiaofei Zhang, Xun Tian, Bei Cheng, Bingjian Lu, Xiao Yu, Suqiu Lan, Weiguo Lu, Ding Ma, Xiaodong Cheng, Xing Xie
OBJECTIVES: We aimed to assess the performance of DH3 human papillomavirus (HPV) assay, a newly developed hybrid capture technique that detects 14 high-risk HPVs with type 16/18 genotyping, as a primary test in cervical cancer screening. METHODS: Totally 11,356 Chinese women aged 21-65 years participated in a cervical cancer screening program using cytology (Thinprep, Hologic) and HPV testing (Cobas 4800 Test, Roche). Residual samples were used to detect HPV by DH3 HPV...
March 5, 2018: Clinical Microbiology and Infection
Judit Danis, Luca Janovák, Barbara Gubán, Anikó Göblös, Kornélia Szabó, Lajos Kemény, Zsuzsanna Bata-Csörgő, Márta Széll
Keratinocytes are non-professional immune cells contributing actively to innate immune responses partially by reacting to a wide range of molecular patterns by activating pattern recognition receptors. Cytosolic nucleotide fragments as pathogen- or self-derived trigger factors are activating inflammasomes and inducing anti-viral signal transduction pathways as well as inducing expression of inflammatory cytokines. We aimed to compare the induced inflammatory reactions in three keratinocyte cell types-normal human epidermal keratinocytes, the HaCaT cell line and the HPV-KER cell line-upon exposure to the synthetic RNA and DNA analogues poly(I:C) and poly(dA:dT) to reveal the underlying signaling events...
March 8, 2018: International Journal of Molecular Sciences
Fatma Beyazit, Fatma Sılan, Meryem Gencer, Buket Aydin, Barış Paksoy, Mesut A Unsal, Ozturk Ozdemir
OBJECTIVES: Cervical cancer is the second most common type of cancer for women worldwide with a great proportion proved to be related to human papillomavirus (HPV) infection. As infection with HPV is the strongest risk factor for cervical neoplasia, detection of HPV genotypes in cervical and vaginal specimens of women with normal and abnormal cytology seems to be of paramount importance in cervical cancer screening. The objective of the study is to evaluate the prevalence and HPV genotypes among women with normal or abnormal Pap smear tests...
2018: Ginekologia Polska
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