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https://www.readbyqxmd.com/read/29353074/diagnostic-value-of-human-papillomavirus-hpv-16-and-hpv18-viral-loads-for-the-detection-of-high-grade-cervical-intraepithelial-neoplasia-cin2-in-a-cohort-of-african-women-living-with-hiv
#1
Michel Segondy, Jean Ngou, Helen Kelly, Tanvier Omar, Olga Goumbri-Lompo, Sylviane Doutre, Philippe Mayaud, Marie-Noelle Didelot
BACKGROUND: African women living with HIV (WLHIV) are at high risk of cervical cancer but rarely adequately screened. Better strategies enabling identification of WLHIV with high-grade cervical intraepithelial lesions (CIN2+) are required. OBJECTIVES: To investigate the diagnostic value of HPV16 and HPV18 viral loads in a cohort of African WLHIV. DESIGN: HPV16 and HPV18 viral loads were determined by quantitation of the E6 gene DNA by real-time PCR in cervical specimens collected at baseline and endline (16 months) from 245 African WLHIV positive for HPV16 or/and HPV18...
January 16, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29350329/detection-of-human-papillomavirus-dna-in-tumors-from-rwandese-breast-cancer-patients
#2
REVIEW
Thierry Habyarimana, Mohammed Attaleb, Jean Baptiste Mazarati, Youssef Bakri, Mohammed El Mzibri
BACKGROUND: During the last decades, a great interest was given to viral etiology of breast cancer. Indeed, due to recent technical improvements and some encouraging new results, it has been a resurgence of interest in the possibility that a substantial proportion of human breast cancers may be caused by viral infections. High-risk genotypes of human papillomavirus (HPV) have been found in breast cancer cases. In the present study, we aimed to assess the presence of HPV DNA in breast cancer cases from Rwanda and to evaluate the association between HPV infection and clinico-pathological features...
January 19, 2018: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/29344860/laser-microdissection-for-human-papillomavirus-hpv-genotyping-attribution-and-methylation-pattern-analyses-of-squamous-intraepithelial-lesions
#3
Monica Molano, Suzanne M Garland, Alyssa M Cornall
Human papillomavirus (HPV) is a nearly ubiquitous infectious organism. It is estimated that 80% of sexually active adults will be exposed to anogenital HPVs in their lifetime, and detection of multiple genotypes in an anogenital sample is common. Detection and genotyping of HPV is usually performed by DNA testing, and less frequently by mRNA testing. HPV genotype testing and characterization of DNA methylation patterns of HPV-related lesions can provide important biological, epidemiological, and potentially relevant clinical information in individuals and populations...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344211/prevalence-of-hpv-infection-among-hiv-positive-and-hiv-negative-women-in-central-eastern-italy-strategies-of-prevention
#4
Edoardo Tartaglia, Katia Falasca, Jacopo Vecchiet, Giovanna Paola Sabusco, Giovanna Picciano, Roberto Di Marco, Claudio Ucciferri
The present cross-sectional-study aimed to determine the prevalence of human papillomavirus (HPV)-genotypes among human immunodeficiency virus (HIV)-positive and -negative women in Central/Eastern Italy, and to identify the optimal strategies for effective HPV-prevention in each group. A representative sample of HIV-negative (150/200) and -positive (50/200) women, who underwent cervico-vaginal-swabbing. Swabs were analysed for a cytological screening and for a HPV-DNA-genotyping-test. A total of 66/200 swabs resulted HPV-positive...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344081/hpv-infection-and-p16-expression-in-oral-and-oropharyngeal-cancer-in-kazakhstan
#5
Dauren Adilbay, Galim Adilbayev, Gulzhan Kidirbayeva, Viktoria Shipilova, Zhanat Sadyk, Gulsum Koyanbekova, Ekaterina Sokolenko, Jan Klozar
Background: Human papillomavirus (HPV) is an important etiologic factor in different cancers of anogenital region and also in a fraction of head and neck cancers (HNC) particularly oropharyngeal tumors. The HPV16 genotype associated with the majority of HPV-related head and neck carcinomas. Th incidence of oropharyngeal cancer is increasing in many countries, and the rate of HPV positive tumors is about 70% in Europe and North America. Little known about the prevalence of HPV in HNC in Central Asia...
2018: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/29341505/stability-integrity-and-recovery-rate-of-cellular-nucleic-acids-preserved-in-a-new-liquid-based-cytology-medium
#6
Guodong Zhao, Zhaocheng Liu, Yaping Tian, Minsheng Zhu, Shouli Wang, Hui Wang, Shen Gao, Yong Ma, Minxue Zheng
BACKGROUND: Liquid-based cytology (LBC) has replaced the conventional Papanicolaou test in cervical cancer screening. The cervical swab specimens collected in LBC media can also be used for additional analyses including high-risk HPV (HR-HPV) test, DNA methylation analysis, and HPV E6/E7 mRNA test. METHODS: The stability, integrity, and recovery rate of cellular DNA and RNA after storage at different conditions were evaluated by a quantitative real-time PCR (qPCR) based HR-HPV test, reverse transcription qPCR (RT-qPCR), and agarose gel electrophoresis...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29337161/cellular-redox-cancer-and-human-papillomavirus
#7
REVIEW
Alfredo Cruz-Gregorio, Joaquín Manzo-Merino, Marcela Lizano
High-risk Human Papillomavirus (HR-HPV) is the causative agent of different human cancers. A persistent HR-HPV infection alters several cellular processes involved in cell proliferation, apoptosis, immune evasion, genomic instability and transformation. Cumulative evidence from past studies indicates that HR-HPV proteins are associated with oxidative stress (OS) and has been proposed as a risk factor for cancer development. Reactive oxygen and nitrogen species (RONS) regulate a plethora of processes inducing cellular proliferation, differentiation and death...
January 11, 2018: Virus Research
https://www.readbyqxmd.com/read/29334204/detection-of-chlamydia-trachomatis-in-pap-smear-samples-from-south-khorasan-province-of-iran
#8
Davod Javanmard, Mahmoodreza Behravan, Malaknaz Ghannadkafi, Alireza Salehabadi, Masood Ziaee, Mohammad Hasan Namaei
Background: Chlamydia trachomatis (CT), the most common bacterial sexually transmitted infection (STI), leads to pelvic inflammatory disease, infertility and chronic pelvic pain in women as well as an increased risk of vertical transmission, conjunctivitis and pneumonitis in infants. It may also be a co-factor along with human papillomavirus (HPV) in cervical cancer progression. We aimed to determine the prevalence of CT genotypes in genital specimens of women from South Khorasan, Iran and to test the association between CT and cytology statistics...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29330557/linear-doggybone-dna-vaccine-induces-similar-immunological-responses-to-conventional-plasmid-dna-independently-of-immune-recognition-by-tlr9-in-a-pre-clinical-model
#9
Alex Allen, Chuan Wang, Lisa J Caproni, Gessa Sugiyarto, Elena Harden, Leon R Douglas, Patrick J Duriez, Kinga Karbowniczek, Jon Extance, Paul J Rothwell, Ifeayinwa Orefo, John P Tite, Freda K Stevenson, Christian H Ottensmeier, Natalia Savelyeva
Vaccination with DNA that encodes cancer antigens is a simple and convenient way to raise immunity against cancer and has already shown promise in the clinical setting. Conventional plasmid DNA is commonly used which together with the encoded antigen also includes bacterial immunostimulatory CpG motifs to target the DNA sensor Toll-like receptor 9. Recently DNA vaccines using doggybone DNA (dbDNA™), have been developed without the use of bacteria. The cell-free process relies on the use of Phi29 DNA polymerase to amplify the template followed by protelomerase TelN to complete individual closed linear DNA...
January 12, 2018: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/29329337/mitochondrial-dna-ancestry-hpv-infection-and-the-risk-of-cervical-cancer-in-a-multiethnic-population-of-northeastern-argentina
#10
Ines Badano, Daiana J Sanabria, Maria E Totaro, Samara Rubinstein, Juan A Gili, Domingo J Liotta, Maria A Picconi, Rodolfo H Campos, Theodore G Schurr
BACKGROUND: Misiones Province in northeastern Argentina is considered to be a region with a high prevalence of HPV infection and a high mortality rate due to cervical cancer. The reasons for this epidemiological trend are not completely understood. To gain insight into this problem, we explored the relationship between mitochondrial DNA (mtDNA) ancestry, HPV infection, and development of cervical lesions/cancer in women from the city of Posadas in Misiones Province. METHODS: Two hundred and sixty-one women, including 92 cases of patients diagnosed with cervical lesions and 169 controls, were analyzed...
2018: PloS One
https://www.readbyqxmd.com/read/29328473/detection-of-high-grade-neoplasia-in-air-dried-cervical-pap-smears-by-a-microrna-based-classifier
#11
Mikhail K Ivanov, Sergei E Titov, Sergei A Glushkov, Victoria V Dzyubenko, Anastasia V Malek, Polina A Arkhangelskaya, Roman B Samsonov, Andrey A Mikhetko, Elena V Bakhidze, Igor V Berlev, Nikolay N Kolesnikov
Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed...
January 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29324843/beta-hpv38-oncoproteins-act-with-a-hit-and-run-mechanism-in-ultraviolet-radiation-induced-skin-carcinogenesis-in-mice
#12
Daniele Viarisio, Karin Müller-Decker, Rosita Accardi, Alexis Robitaille, Matthias Dürst, Katrin Beer, Lars Jansen, Christa Flechtenmacher, Matthias Bozza, Richard Harbottle, Catherine Voegele, Maude Ardin, Jiri Zavadil, Sandra Caldeira, Lutz Gissmann, Massimo Tommasino
Cutaneous beta human papillomavirus (HPV) types are suspected to be involved, together with ultraviolet (UV) radiation, in the development of non-melanoma skin cancer (NMSC). Studies in in vitro and in vivo experimental models have highlighted the transforming properties of beta HPV E6 and E7 oncoproteins. However, epidemiological findings indicate that beta HPV types may be required only at an initial stage of carcinogenesis, and may become dispensable after full establishment of NMSC. Here, we further investigate the potential role of beta HPVs in NMSC using a Cre-loxP-based transgenic (Tg) mouse model that expresses beta HPV38 E6 and E7 oncogenes in the basal layer of the skin epidermis and is highly susceptible to UV-induced carcinogenesis...
January 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29322839/feasibility-study-of-a-human-papillomavirus-e6-and-e7-oncoprotein-test-for-the-diagnosis-of-cervical-precancer-and-cancer
#13
Jin-Jun Zhang, Xin-Chun Cao, Xiang-Yu Zheng, Hai-Ying Wang, Yong-Wei Li
Objective To evaluate the clinical value of human papillomavirus (HPV) E6 and E7 oncoprotein (HPV E6/E7) detection in the early screening of cervical cancer. Methods This prospective study evaluated all patients with suspected cervical intraepithelial neoplasia (CIN) as identified by the presence of at least one positive indicator from a ThinPrep cytologic test (TCT) and/or a Hybrid Capture 2 (HC2) HPV DNA test. The levels of E6/E7 oncoproteins were determined using Western blot analysis. The diagnostic value of the HPV E6/E7 protein assay was compared with the clinical diagnosis from TCT, HC2 and the gold standard of cervical biopsy histology...
January 1, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29322795/analysis-of-dna-methylation-and-microrna-expression-in-nut-nuclear-protein-in-testis-midline-carcinoma-of-the-sinonasal-tract-a-clinicopathological-immunohistochemical-and-molecular-genetic-study
#14
J Laco, H Kovarikova, M Chmelarova, H Vosmikova, K Sieglova, I Bubancova, P Dundr, K Nemejcova, J Michalek, P Celakovsky, R Mottl, I Sirak, M Vosmik, I Marek, T Geryk, J Mejzlik, J Satankova, A Ryska
The aim of this study was a detailed clinicopathological investigation of sinonasal NUT midline carcinoma (NMC), including analysis of DNA methylation and microRNA (miRNA) expression. Three (5%) cases of NMC were detected among 56 sinonasal carcinomas using immunohistochemical screening and confirmed by fluorescence in situ hybridization. The series comprised 2 males and 1 female, aged 46, 60, and 65 years. Two tumors arose in the nasal cavity and one in the maxillary sinus. The neoplasms were staged pT1, pT3, and pT4a (all cN0M0)...
2018: Neoplasma
https://www.readbyqxmd.com/read/29321327/human-papillomavirus-16-infection-induces-vap-dependent-endosomal-tubulation
#15
Abida Siddiqa, Paola Massimi, David Pim, Justyna Broniarczyk, Lawrence Banks
Human Papillomavirus (HPV) infection involves complex interactions with the endocytic transport machinery, which ultimately facilitates the entry of the incoming viral genomes into the trans-Golgi network and their subsequent nuclear entry during mitosis. The endosomal pathway is a highly dynamic intracellular transport system, which consists of vesicular compartments and tubular extensions, although it is currently unclear whether incoming viruses specifically alter the endocytic machinery. In this study, using MICAL-L1 as a marker for tubulating endosomes, we show that incoming HPV-16 virions induce a profound alteration in global levels of endocytic tubulation...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29316817/therapeutic-dna-vaccines-for-human-papillomavirus-and-associated-diseases
#16
Max A Cheng, Emily Farmer, Claire Huang, John Lin, Chien-Fu Hung, T-C Wu
Human papillomavirus (HPV) has long been recognized as the causative agent of cervical cancer. High-risk HPV types 16 and 18 alone, are responsible for over 70% of all cases of cervical cancers. More recently, HPV has been identified as an etiological factor for several other forms of cancers, including oropharyngeal, anogenital, and skin. Thus, the association of HPV with these malignancies creates opportunity for the control of these HPV lesions and HPV-associated malignancies through immunization. Strategies to prevent or to therapeutically treat HPV infections have been developed and are still pushing innovative boundaries...
January 9, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29315288/the-promise-of-immunotherapy-in-anal-squamous-cell-carcinoma-a-novel-approach-for-an-orphan-disease
#17
Benny Johnson, Cathy Eng
An estimated 8200 men and women in the United States will receive a diagnosis of squamous cell carcinoma of the anal canal (SCCA) in 2017. Although SCCA is rare, accounting for 2.6% of gastrointestinal cancers, its incidence rate has been steadily increasing over the last few decades in the United States and around the world. More than 90% of cases of SCCA occur in the context of prior human papillomavirus (HPV) infection. To date, preventive vaccinations against HPV remain markedly underutilized. Most patients who have SCCA present with locoregional disease that is cured with chemoradiation...
December 2017: Clinical Advances in Hematology & Oncology: H&O
https://www.readbyqxmd.com/read/29314780/hpv-16-targeted-dna-vaccine-expression-the-role-of-purification
#18
Ana M Almeida, Joana Tomás, Patrícia Pereira, João A Queiroz, Fani Sousa, Ângela Sousa
DNA vaccines have come to light in the last decades as an alternative method to prevent many infectious diseases, but they can also be used for the treatment of specific diseases, such as cervical cancer caused by Human Papillomavirus (HPV). This virus produces E6 and E7 oncoproteins, which alter the cell cycle regulation and can interfere with the DNA repairing system. These features can ultimately lead to the progression of cervical cancer, after cell infection by HPV. Thus, the development of a DNA vaccine targeting both proteins arises as an interesting option in the treatment of this pathology...
January 5, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29314149/uniplex-e6-e7-pcr-method-detecting-e6-or-e7-genes-in-39-human-papillomavirus-types
#19
Mitsuaki Okodo, Kaori Okayama, Koji Teruya, Toshiyuki Sasagawa
We have developed a new human papillomavirus (HPV) assay using the uniplex E6/E7 polymerase chain reaction (PCR) method, which is able to detect E6 and E7 genes in 39 HPV types. We validated the assay for sensitivity and specificity using cloned HPV DNA and clinical samples. A comparative study using Genosearch-31 (GS-31) to determine HPV genotypes in clinical samples was also performed. E6 or E7 genes, measured by uniplex E6/E7 PCR, were detectable in 15 low-risk (HPV-6, -11, -40, -42, -44, -54, -55, -61, -62, -71, -74, -81, -84, -89, -90), 11 intermediate-risk (HPV-26, -30, -34, -53, -66, -67, -69, -70, -73, -82, -85), and 13 high-risk (HR) HPV types...
January 4, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29310590/oral-human-papillomavirus-dna-detection-in-hiv-positive-men-prevalence-predictors-and-co-occurrence-at-anal-site
#20
Alessandra Vergori, Anna Rosa Garbuglia, Pierluca Piselli, Franca Del Nonno, Catia Sias, Federico Lupi, Daniele Lapa, Andrea Baiocchini, Claudia Cimaglia, Marco Gentile, Andrea Antinori, Maria Capobianchi, Adriana Ammassari
BACKGROUND: HIV-positive patients carry an increased risk of HPV infection and associated cancers. Therefore, prevalence and patterns of HPV infection at different anatomical sites, as well as theoretical protection of nonavalent vaccine should be investigated. Aim was to describe prevalence and predictors of oral HPV detection in HIV-positive men, with attention to nonavalent vaccine-targeted HPV types. Further, co-occurrence of HPV DNA at oral cavity and at anal site was assessed. METHODS: This cross-sectional, clinic-based study included 305 HIV-positive males (85...
January 8, 2018: BMC Infectious Diseases
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