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https://www.readbyqxmd.com/read/28530265/papillary-squamous-cell-carcinoma-of-the-palatine-tonsil-a-rare-cancer-of-the-head-and-neck
#1
A Serra, R Caltabiano, G Scalia, S Palmucci, P Di Mauro, S Cocuzza
Papillary squamous neoplasms of the upper respiratory tract are rare variants of squamous cell carcinomas. They are characterised by an exophytic, papillary growth and generally have favourable prognosis. The tumour has been described in the upper aerodigestive tract. In this context, most common sites of involvement are the larynx and hypopharynx, and rarely the oral cavity and oropharynx. The limited studies and small number of published cases of papillary squamous cell carcinoma of the palatine tonsil led us to make a complete analysis of this tumour by analysing the clinical, histological, radiological, virological and therapeutic aspects that are not always present in the literature...
May 22, 2017: Acta Otorhinolaryngologica Italica
https://www.readbyqxmd.com/read/28528690/clinical-relevance-and-implications-of-hpv-induced-neoplasia-in-different-anatomical-locations
#2
REVIEW
Elena Sophie Prigge, Magnus von Knebel Doeberitz, Miriam Reuschenbach
Human papillomaviruses (HPV) are widespread DNA viruses that can infect epithelial cells of the skin and mucosa. Most HPV infections remain clinically unapparent and clear spontaneously. In few cases, however, HPV infections persist and can cause benign and malignant neoplasms at different anatomic locations. Malignant HPV-induced neoplasms are caused by distinct types of HPV (oncogenic or high-risk (HR) HPV types) and present in the anogenital (anus, penis, uterine cervix, vagina and vulva) and head and neck (particularly oropharynx) region...
April 2017: Mutation Research
https://www.readbyqxmd.com/read/28528689/human-papillomaviruses-in-epigenetic-regulations
#3
REVIEW
Julia Durzynska, Krzysztof Lesniewicz, Elzbieta Poreba
Human Papillomaviruses (HPVs) are double-stranded DNA viruses, that infect epithelial cells and are etiologically involved in the development of human cancer. Today, over 200 types of human papillomaviruses are known. They are divided into low-risk and high-risk HPVs depending on their potential to induce carcinogenesis, driven by two major viral oncoproteins, E6 and E7. By interacting with cellular partners, these proteins are involved in interdependent viral and cell cycles in stratified differentiating epithelium, and concomitantly induce epigenetic changes in infected cells and those undergoing malignant transformation...
April 2017: Mutation Research
https://www.readbyqxmd.com/read/28527674/analysis-of-full-coding-sequence-of-the-tp53-gene-in-invasive-vulvar-cancers-implications-for-therapy
#4
Karl Kashofer, Sigrid Regauer
OBJECTIVE: This study evaluates the frequency and type of TP53 gene mutations and HPV status in 72 consecutively diagnosed primary invasive vulvar squamous cell carcinomas (SCC) during the past 5years. METHODS: DNA of formalin-fixed and paraffin embedded tumour tissue was analysed for 32 HPV subtypes and the full coding sequence of the TP53 gene, and correlated with results of p53 immunohistochemistry. RESULTS: 13/72 (18%) cancers were HPV-induced squamous cell carcinomas, of which 1/13 (8%) carcinoma harboured a somatic TP53 mutation...
May 17, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28525363/targeted-sequencing-of-tonsillar-and-base-of-tongue-cancer-and-human-papillomavirus-positive-unknown-primary-of-the-head-and-neck-reveals-prognostic-effects-of-mutated-fgfr3
#5
Cinzia Bersani, Lars Sivars, Linnea Haeggblom, Sebastian DiLorenzo, Michael Mints, Andreas Ährlund-Richter, Nikolaos Tertipis, Eva Munck-Wikland, Anders Näsman, Torbjörn Ramqvist, Tina Dalianis
BACKGROUND: Human papillomavirus positive (HPV+) tonsillar cancer (TSCC), base of tongue cancer (BOTSCC) and unknown primary cancer of the head and neck (HNCUP) have better outcome than corresponding HPV- cancers. To find predictive markers for response to treatment, and correlations and differences in mutated oncogenes and suppressor genes between HPV+ TSCC/BOTSSCC and HPV+ HNCUP and HPV- TSCC/BOTSCC targeted next-generation sequencing was performed of frequently mutated regions in 50 cancer related genes...
February 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28522591/inhibition-of-aurora-a-and-aurora-b-is-required-for-the-sensitivity-of-hpv-driven-cervical-cancers-to-aurora-kinase-inhibitors
#6
David Martin, Sora Fallaha, Martina Proctor, Alexander Stevenson, Lewis Perrin, Nigel McMillan, Brian Gabrielli
The activity and efficacy of Aurora inhibitors have been reported in a wide range of cancer types.   The most prominent Aurora inhibitor is Alisertib, an investigational Aurora inhibitor that has been the subject of more than 30 clinical trials.   Alisertib has inhibitory activity against both Aurora A and B, although it is considered to be primarily an Aurora A inhibitor in vivo.  Here we show that Alisertib inhibits both Aurora A and B in vivo in preclinical models of HPV-driven cervical cancer, and that it is the inhibition of Aurora A and B that provide the selectivity and efficacy of this drug in vivo in this disease setting...
May 18, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28521481/increased-methylation-of-human-papillomavirus-type-16-dna-is-associated-with-the-severity-of-cervical-lesions-in-infected-females-from-northeast-china
#7
Wei Wang, Zhengrong Sun, Jianhua Liu, Guili Wang, Zhitao Lu, Weiqiang Zhou, Te Qi, Qiang Ruan
Hypermethylation of the cytosine-phosphate-guanine (CpG) sites located at the 3'-major capsid protein L1 (3'L1) and the long control region (LCR) of the human papillomavirus (HPV) genome may be associated with the progression of cervical cancer (CC). However, the methylation status of the LCR of HPV type 16 DNA remains to be elucidated in an infected Chinese population. The aim of the present study was to investigate the association between methylation of the HPV 16 L1 gene and LCR, and the severity of cervical lesions in infected female patients...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28521315/p16-ki-67-immunocytochemistry-in-gynecological-cytology-limitations-in-practice
#8
Peter Ziemke
OBJECTIVE: Immunochemical detection of the protein p16INK4a in cervical cytology is used in combination with Ki-67. Cells positive for both proteins are certain to have been transformed by high-risk HPV. p16/Ki-67 immunocytochemistry provides a significant improvement in specificity over cytology and HPV DNA testing. However, p16/Ki-67 immunocytochemistry also has its limitations. STUDY DESIGN: The research is based on the follow-up of 1,131 patients for whom p16/Ki-67 immunocytochemistry was performed with cytology...
May 19, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28521287/5azadc-treatment-upregulates-mir-375-level-and-represses-hpv16-e6-expression
#9
Adrien Morel, Aurélie Baguet, Jérôme Perrard, Caroline Demeret, Elise Jacquin, David Guenat, Christiane Mougin, Jean-Luc Prétet
High risk human papillomaviruses are the etiological agents of cervical cancer and HPV16 is the most oncogenic genotype. Immortalization and transformation of infected cells requires the overexpression of the two viral oncoproteins E6 and E7 following HPV DNA integration into the host cell genome. Integration often leads to the loss of the E2 open reading frame and the corresponding protein can no longer act as a transcriptional repressor on p97 promoter. Recently, it has been proposed that long control region methylation also contributes to the regulation of E6/E7 expression...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28515303/spontaneous-and-vaccine-induced-clearance-of-mus-musculus-papillomavirus-type-1-mmupv1-muspv1-infection
#10
Rosie T Jiang, Joshua W Wang, Shiwen Peng, Tsui-Chin Huang, Chenguang Wang, Fabiana Cannella, Yung-Nien Chang, Raphael P Viscidi, Simon R A Best, Chien-Fu Hung, Richard B S Roden
Mus musculus Papillomavirus1 (MmuPV1/MusPV1) induces persistent papillomas in immunodeficient mice but not common laboratory strains. To facilitate study of immune control, we sought an outbred and immune competent laboratory mouse strain in which persistent papillomas could be established. We found that challenge of SKH1 mice (Crl:SKH1-Hrhr) by scarification on their tail with MmuPV1 resulted in three clinical outcomes: 1) persistent (>2 months) papillomas (∼20%), 2) transient papillomas that spontaneously regress typically within 2 months (∼15%), 3) no visible papillomas and viral clearance (∼65%)...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28515214/assessment-of-a-new-lower-cost-real-time-pcr-detection-assay-for-high-risk-hpv-useful-for-cervical-screening-in-limited-resource-settings
#11
Joel Fokom Domgue, Mark Schiffman, Nicolas H Wentzensen, Julia C Gage, Philip E Castle, Tina R Raine-Bennett, Barbara Fetterman, Thomas Lorey, Nancy E Poitras, Brian Befano, Yi Xie, Lais S Miachon, Michael Dean
Background Inexpensive and easy-to-perform HPV tests are needed for primary cervical cancer screening in lower-resource regions.Methods In a convenience sample of 516 residual exfoliative cervical specimens from the Kaiser Permanente Northern California and US National Cancer Institute Persistence and Progression study, we compared agreement and clinical performance of a simple, inexpensive real-time PCR assay for detection of 13 carcinogenic HPV types (H13, Hybribio, Hong Kong) that is marketed in limited resource settings, to previous testing by HC2 (Qiagen, Germantown, MD) and Onclarity (BD Diagnostics, Sparks, MD)...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28515108/a-comparison-of-the-natural-history-of-hpv-infection-and-cervical-abnormalities-among-hiv-positive-and-hiv-negative-women-in-senegal-africa
#12
Hilary K Whitham, Stephen E Hawes, Haitao Chu, J Michael Oakes, Alan R Lifson, Nancy B Kiviat, Papa Salif Sow, Geoffrey S Gottlieb, Selly Ba, Marie P Sy, Shalini L Kulasingam
Background: There is evidence of an interaction between HIV and human papillomavirus (HPV) resulting in increased HPV-associated morbidity and cancer mortality among HIV-positive women. This study aims to determine how the natural history of cervical HPV infection differs by HIV status.Methods: A total of 1,320 women (47% were positive for HIV-1 and/or HIV-2) were followed for an average of two years in Senegal, West Africa between 1994 and 2010. Cytology (with a sub-sample of histology) and HPV DNA testing were performed at approximately 4-month intervals yielding data from over 7,900 clinic visits...
May 17, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28508913/digitally-encoded-silica-microparticles-for-multiplexed-nucleic-acid-detection
#13
Weiwei Xu, Chao Chen, Xiaodong Ma, Lihua Yuan, Shenquan Liu, Kexiao Zheng, Jiong Li
A robust high throughput suspension array was proposed to analyze biomolecules. To exploit its capacity for nucleic acid detection, we designed a multiplex nested asymmetric PCR (MNAS PCR) that can produce single stranded DNA (ssDNA) efficiently without complicated optimization. Multiplexed HPV genotyping was demonstrated with high selectivity, high sensitivity and rapid hybridization within 20 minutes.
May 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28508693/a-metabolite-profiling-method-for-diagnosis-of-precancerous-cervical-lesions-and-hpv-persistence
#14
Heather Walker, Michael Burrell, Janet Flatley, Hilary Powers
AIM: With the advent of rapid metabolic profiling techniques and of portable mass spectrometers we examined whether cells distinguished by their cytology and persistence of human papillomavirus infection, could be easily differentiated by their metabolite profile. MATERIALS & METHODS: Direct injection electrospray mass spectrometry was used in a nontargeted double-blind experiment. Samples were collected from women diagnosed with one of two grades of cervical cytology and exhibiting either human papilloma virus persistence or clearance...
May 16, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28500655/discovery-and-validation-of-candidate-host-dna-methylation-markers-for-detection-of-cervical-precancer-and-cancer
#15
Megan A Clarke, Patricia Luhn, Julia C Gage, Clara Bodelon, S Terence Dunn, Joan Walker, Rosemary Zuna, Stephen Hewitt, J Keith Killian, Liying Yan, Andrew Miller, Mark Schiffman, Nicolas Wentzensen
Human papillomavirus (HPV) testing has been recently introduced as an alternative to cytology for cervical cancer screening. However, since most HPV infections clear without causing clinically relevant lesions, additional triage tests are required to identify women who are at high risk of developing cancer. We performed DNA methylation profiling on formalin-fixed, paraffin-embedded tissue specimens from women with benign HPV16 infection and histologically confirmed cervical intraepithelial neoplasia grade 3 (CIN3), and cancer using a bead-based microarray covering 1,500 CpG sites in over 800 genes...
May 13, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28498639/three-year-risk-of-high-grade-cin-for-women-aged-30-years-or-older-who-undergo-baseline-pap-cytology-and-hpv-co-screening
#16
Ming Guo, Abha Khanna, Jianping Wang, Marilyn A Dawlett, Teresa L Kologinczak, Genevieve R Lyons, Roland L Bassett, Nour Sneige, Yun Gong, Therese B Bevers
BACKGROUND: Papanicolaou (Pap) cytology and high-risk human papillomavirus (HPV) DNA cotesting for women aged ≥30 years are recommended for the prevention of cervical cancer. The objective of the current study was to evaluate the efficacy of this cotesting for predicting the risk of high-grade cervical intraepithelial neoplasia 3 (CIN3) during a 3-year follow-up period. METHODS: A retrospective database search identified women aged ≥30 years who had baseline HPV and Pap cytology cotesting results in 2007 or 2008 and for whom 3-year follow-up results were available...
May 12, 2017: Cancer
https://www.readbyqxmd.com/read/28492521/coinjection-of-il2-dna-enhances-e7-specific-antitumor-immunity-elicited-by-intravaginal-therapeutic-hpv-dna-vaccination-with-electroporation
#17
Y Sun, S Peng, A Yang, E Farmer, T-C Wu, Chien-Fu Hung
The generation and use of therapeutic human papillomavirus (HPV) DNA vaccines represent an appealing treatment method against HPV-associated cervical cancer due to their safety and durability. Previously, we created a therapeutic HPV DNA vaccine candidate by linking the HPV16-E7 DNA sequence to calreticulin (CRT/E7), which we showed could generate significant E7-specific cytotoxic T lymphocyte (CTL)-mediated anti-tumor immune responses against HPV16 oncogenes expressing murine tumor model TC-1. Here we assess the therapeutic efficacy of intravaginal immunization with pcDNA3-CRT/E7 followed by electroporation...
May 11, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28487502/pdcd1-pd-1-promoter-methylation-predicts-outcome-in-head-and-neck-squamous-cell-carcinoma-patients
#18
Diane Goltz, Heidrun Gevensleben, Joern Dietrich, Friederike Schroeck, Luka de Vos, Freya Droege, Glen Kristiansen, Andreas Schroeck, Jennifer Landsberg, Friedrich Bootz, Dimo Dietrich
BACKGROUND: Biomarkers that facilitate the prediction of disease recurrence in head and neck squamous cell carcinoma (HNSCC) may enable physicians to personalize treatment. In the current study, DNA promoter methylation of programmed cell death 1 (PDCD1, PD-1) was evaluated as a prognostic biomarker in HNSCC patients. RESULTS: High PDCD1 methylation (mPDCD1) was associated with a significantly shorter overall survival after surgical resection in both the discovery (HR = 2...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28486384/human-papillomavirus-hpv-genotypes-in-condylomas-intraepithelial-neoplasia-and-invasive-carcinoma-of-the-penis-using-laser-capture-microdissection-lcm-pcr-a-study-of-191-lesions-in-43-patients
#19
María J Fernández-Nestosa, Nuria Guimerà, Diego F Sanchez, Sofía Cañete-Portillo, Elsa F Velazquez, David Jenkins, Wim Quint, Antonio L Cubilla
Laser capture microdissection-polymerase chain reaction (LCM-PCR) supported by p16 was used for the first time to demonstrate human papillomavirus (HPV) DNA in histologically specific penile lesions, which were as follows: squamous hyperplasia (12 lesions, 10 patients), flat lesions (12 lesions, 5 patients), condylomas (26 lesions, 7 patients), penile intraepithelial neoplasia (PeIN) (115 lesions, 43 patients), and invasive squamous cell carcinomas (26 lesions, 26 patients). HPV was detected by whole-tissue section and LCM-PCR...
June 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28484898/virus-induced-cancers-of-the-skin-and-mucosa-are-we-dealing-with-smoking-guns-or-smoke-and-mirrors-in-the-operating-theatre
#20
Peter K C Goon, Patrick K Y Goon, Eunice K H Tan, Robin A F Crawford, Nick J Levell, Holger Sudhoff
INTRODUCTION: Human papillomavirus (HPV) alone is thought to cause ~610,000 cases of cancer per year, and is the dominant aetiological agent for ano-genital (esp. cervical) and head and neck cancers (esp. oropharyngeal). Merkel cell polyomavirus (MCV) is a more recently discovered virus which causes Merkel cell carcinoma, a rare but highly aggressive skin malignancy. METHODS: We explored the available published evidence to see if transmission of live HPV or MCV virus in smoke generated by laser or diathermy was feasible, and would pose an infection risk...
May 8, 2017: Dermatology and Therapy
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