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https://www.readbyqxmd.com/read/28440976/diagnostic-value-of-the-caretm-hpv-test-in-screening-for-cervical-intraepithelial-neoplasia-grade-2-or-worse
#1
Mojgan Karimi Zarchi, Effat Heydari, Afsarolsadat Tabatabaie, Mansour Moghimi, Wesam Kooti
Background: Cervical cancer is the fourth leading cause of cancer death in women worldwide. Persistent infection with a high risk human papillomavirus (HR-HPV) is the main etiological factor, so that early early detection of HR-HPV is very important. The aim of this study was to investigate the efficacy of CareTM HPV, a new method, as compared with Pap smear, PCR, and biopsy for screening purposes. Material and Method: In this cross-sectional study, 200 sexually active women aging from 25-50 years referred to the oncology clinic of Shahid Sodoughi Yazd Hospital in 2015 with a variety of cervix epithelial lesions or a need for colposcopy were enrolled...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28439692/the-presence-and-prognostic-significance-of-human-papillomavirus-in-squamous-cell-carcinoma-of-the-larynx
#2
Evren Erkul, Ismail Yilmaz, Gizem Narli, Mustafa Alparslan Babayigit, Atila Gungor, Dilaver Demirel
The aim of the present study was to evaluate the role of HPV in laryngeal squamous cell carcinoma and correlate it with patients' clinicopathological data. In total, 78 laryngeal squamous cell carcinoma patients enrolled in this study. The presence of genotype-specific HPV DNA was evaluated using Genotyping Assay in formalin-fixed paraffin-embedded tissue which was diagnosed between 2005 and 2015. All samples were also evaluated for p16 immunohistochemical staining. HPV DNA and p16 status were assessed in terms of location, smoking, alcohol consumption, lymph node status, tumor stage, overall survival, disease-free survival, perineural invasion, and vascular invasion retrospectively...
April 21, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28438378/genotyping-of-the-human-papilloma-virus-in-a-group-of-mexican-women-treated-in-a-highly-specialist-hospital-multiple-infections-and-their-potential-transcendence-in-the-current-vaccination-programme
#3
Pablo Romero-Morelos, Arizbett Uribe-Jiménez, Cindy Bandala, Albros Poot-Vélez, Nora Ornelas-Corral, Miriam Rodríguez-Esquivel, Mariana Valdespino-Zavala, Keiko Taniguchi, Daniel Marrero-Rodríguez, Ricardo López-Romero, Mauricio Salcedo
BACKGROUND AND OBJETIVE: Human papilloma virus (HPV) is one of the main risk factors associated with the development of cervical cancer and its precursor lesions. It has been reported that HPV16 and 18 types cover approximately 70% of cervical cancer worldwide; however, significant variation in percentages of HPV infections could be related to specific populations. MATERIALS AND METHODS: Purified DNA of 67 cervical samples were analyzed by Linear Array® HPV genotyping kit...
April 21, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28438294/a-model-using-concomitant-markers-for-predicting-outcome-in-human-papillomavirus-positive-oropharyngeal-cancer
#4
Cinzia Bersani, Michael Mints, Nikolaos Tertipis, Linnea Haeggblom, Lars Sivars, Andreas Ährlund-Richter, Andrea Vlastos, Cecilia Smedberg, Nathalie Grün, Eva Munck-Wikland, Anders Näsman, Torbjörn Ramqvist, Tina Dalianis
OBJECTIVE: Head-neck cancer therapy has become intensified. With radiotherapy alone, 3-year disease-free survival (DFS) is 80% for HPV-positive TSCC/BOTSCC and better for patients with favorable characteristics, suggesting therapy could be tapered for some, decreasing side-effects. Therefore, we built a model to predict progression-free survival for patients with HPV-positive TSCC and BOTSCC. MATERIAL AND METHODS: TSCC/BOTSCC patients treated curatively between 2000 and 2011, with HPV16 DNA/E7 mRNA positive tumors examined for CD8(+) TILs, HPV16 mRNA and HLA class I expression were included...
May 2017: Oral Oncology
https://www.readbyqxmd.com/read/28436064/the-changing-landscape-of-cervical-screening-what-does-the-future-hold-for-primary-care
#5
L Marlow, J Waller
The landscape of cervical cancer prevention is changing in many countries thanks to the introduction of vaccination against high-risk types of human papillomavirus (HPV) and the incorporation of HPV DNA testing into cervical screening algorithms. In addition to this, uptake of screening is falling year on year in the UK and elsewhere. These factors present challenges and opportunities for health professionals working in primary care-in terms of communicating programmatic changes to women; responding to questions about the meaning and implications of HPV test results; and delivering interventions to increase screening uptake...
April 23, 2017: European Journal of Cancer Care
https://www.readbyqxmd.com/read/28431571/unique-lcr-variations-among-lineages-of-hpv16-18-and-45-isolates-from-women-with-normal-cervical-cytology-in-ghana
#6
Adolf K Awua, Richard M K Adanu, Edwin K Wiredu, Edwin A Afari, Vanessa A Zubuch, Richard H Asmah, Alberto Severini
BACKGROUND: In addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28428646/-hpv-vaccination-active-offer-in-an-italian-region
#7
Elisa Terracciano, Gian Loreto D'Alò, Silvia Aquilani, Anna Maria Aversa, Giuseppina Bartolomei, Maria Gabriella Calenda, Raffaele Catapano, Silvio Compagno, Piera Della Rovere, Angelo Fraioli, Roberto Ieraci, Daniela Reggiani, Stefano Sgricia, Antonietta Spadea, Laura Zaratti, Elisabetta Franco
Human Papillomavirus is responsible for 4.8% of cancers, and is the main cause of cervical cancer. Cervical cancer can be reduced by mean of secondary prevention (PAP-test, HPV-DNA test), while through primary prevention (anti-HPV vaccine) the incidence of other HPV-attributable cancers can also be reduced. In Italy, anti-HPV vaccination is part of the immunization schedule in girls since 2008, and in 2017 it was extended to boys. However, vaccine coverage is decreasing nationwide. This study aims to examine anti-HPV vaccination practices in Health care services of Lazio Region, Italy...
January 2017: Igiene e Sanità Pubblica
https://www.readbyqxmd.com/read/28426837/the-high-prevalence-of-hpv-and-hpv16-european-variants-in-cervical-and-anal-samples-of-hiv-seropositive-women-with-normal-pap-test-results
#8
Lays Paula Bondi Volpini, Neide Aparecida Tosato Boldrini, Luciana Bueno de Freitas, Angelica Espinosa Miranda, Liliana Cruz Spano
Human Immunodeficiency Virus (HIV)-seropositive women are more likely to have anogenital cancer, and high risk-HPV (HR-HPV) infection is the main associated factor. Between August 2013 and December 2015, we conducted a descriptive study to determine the HPV genotypes and HPV16 variants in cervical and anal samples of HIV-seropositive women with a normal Pap test. The viral DNA was amplified by PCR using the PGMY09/11 set of primers. Reverse line blot (RLB), restriction fragment length polymorphism (RFLP) and sequencing assays were used to determine the HPV genotypes...
2017: PloS One
https://www.readbyqxmd.com/read/28426487/brief-report-hpv-17-infection-in-darier-disease-with-acrokeratosis-verrucosis-of-hopf
#9
Andrew Matsumoto, Neal Gregory, Peter L Rady, Stephen K Tyring, John A Carlson
The co-existence of Darier disease (DD) and acrokeratosis verruciformis of Hopf (AKV) has been noted for decades and the relationship between the 2 entities remains controversial. Although, it has been shown that both diseases are associated with mutations in ATPA2 gene, it is yet to be determined if they are the same disease, or separate but allelic, or interlinked in some other fashion. Herein, the authors report the case of a 13-year-old girl presenting with shiny flat-topped verruca plana-like papules, on the dorsal hands and feet and red-brown crusted papules on her forehead and along the sides of her neck...
May 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28423694/epstein-barr-virus-ebv-encoded-small-rnas-ebers-associated-with-poor-prognosis-of-head-and-neck-carcinomas
#10
Turunen Aaro, Rautava Jaana, Grénman Reidar, Syrjänen Kari, Syrjänen Stina
BACKGROUND: Epstein-Barr virus (EBV) is the main cause of nasopharyngeal carcinoma (NPC), also found in other head and neck carcinomas (HNSCCs) where its role remains controversial. RESULTS: EBV was found in 80% and 21% of the samples with PCR and ISH (in cancer cells), respectively. Eight of ISH-positive samples were not NPCs. EBER-RNA detection in carcinoma cells was associated with worse prognosis, whether or not NPCs were included. HPV/EBV and HSV/HPV coinfections associated with a shorter survival...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421207/detection-of-human-papillomavirus-genotypes-and-epstein-barr-virus-in-nasopharyngeal-carcinomas-at-the-korle-bu-teaching-hospital-ghana
#11
Du-Bois Asante, Richard Harry Asmah, Andrew Anthony Adjei, Foster Kyei, David Larbi Simpong, Charles Addoquaye Brown, Richard Kwasi Gyasi
Nasopharyngeal carcinomas (NPC) are endemic in Far East Asia and commonly harbour Epstein-Barr virus (EBV) which is known to serve as a key oncogenic promoter. Human papillomavirus (HPV) is known to contribute to the pathogenesis of NPC. However, in Ghana these two viruses have not been linked to NPC prevalence. This study was designed to determine the HPV genotypes and EBV involved in NPC tissue biopsies. A retrospective study design involving 72 formalin-fixed paraffin-embedded tissue (FFPET) samples of NPC from 2006 to 2012 were retrieved from the Department of Pathology, University of Ghana School of Biomedical and Allied Health Sciences...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28415784/misregulation-of-dna-damage-repair-pathways-in-hpv-positive-head-and-neck-squamous-cell-carcinoma-contributes-to-cellular-radiosensitivity
#12
Catherine M Nickson, Parisa Moori, Rachel J Carter, Carlos P Rubbi, Jason L Parsons
Patients with human papillomavirus type 16 (HPV)-associated oropharyngeal squamous cell carcinomas (OPSCC) display increased sensitivity to radiotherapy and improved survival rates in comparison to HPV-negative forms of the disease. However the cellular mechanisms responsible for this characteristic difference are unclear. Here, we have investigated the contribution of DNA damage repair pathways to the in vitro radiosensitivity of OPSCC cell lines. We demonstrate that two HPV-positive OPSCC cells are indeed more radiosensitive than two HPV-negative OPSCC cells, which correlates with reduced efficiency for the repair of ionising radiation (IR)-induced DNA double strand breaks (DSB)...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415759/kshv-co-infection-down-regulates-hpv16-e6-and-e7-from-cervical-cancer-cells
#13
Lu Dai, Yueyu Cao, Wei Jiang, Jovanny Zabaleta, Zhongmin Liu, Jing Qiao, Zhiqiang Qin
High-risk human papillomavirus (HPV) infection is the etiological agent of some malignancies such as cervical, oral and oropharyngeal cancers. Kaposi sarcoma-associated herpesvirus (KSHV) represents a principal causative agent of several human cancers arising in those immunocompromised patients. Interestingly, KSHV DNA has been detected in the oral cavity and the female genital tract, although its detection rate in cervical samples is very low and few reports are about KSHV/HPV co-infection. Therefore, it remains unclear about the role of KSHV co-infection in the development of HPV-related neoplasias...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28409676/analysis-of-risk-factors-for-persistent-infection-of-asymptomatic-women-with-high-risk-human-papilloma-virus
#14
Nianmin Shi, Qiang Lu, Jiao Zhang, Li Li, Junnan Zhang, Fanglei Zhang, Yanhong Dong, Xinyue Zhang, Zheng Zhang
OBJECTIVE: This study aims to prevent persistent infection, reduce the incidence of cervical cancer, and improve women's health by understanding the theoretical basis of the risk factors for continuous infection of asymptomatic women with high-risk human papilloma virus (HPV) strains via information collected, which includes the persistent infection rate and the most prevalent HPV strain types of high risk to asymptomatic women in the high-risk area of cervical cancer in Linfen, Shanxi Province...
April 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28403538/human-papillomavirus-epstein-barr-virus-and-methylation-status-of-p16-ink4a-in-penile-cancer
#15
L A Afonso, F N Carestiato, A A Ornellas, P Ornellas, W M Rocha, T I Cordeiro, D C Lisboa, G Alves, Smb Cavalcanti
Little is known about penile carcinogenesis. The aim of this study was to evaluate the prevalence of HPV and EBV, and the methylation status of p16(ink4a) in penile cancer samples, and to contribute to the understanding of the mechanisms responsible for penile cancer development. HPV DNA was detected in 63.6% of 122 cases, with HPV16 being the most prevalent type. EBV DNA was detected in 47.7%, with EBV-1 being the most prevalent type. HPV/EBV co-infections were found in 27.3% of the cases. Hypermethylation in p16(ink4a) was detected in 64...
April 12, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28403015/hr-hpv-e6-e7-mrna-in-situ-hybridization-validation-against-pcr-dna-in-situ-hybridization-and-p16-immunohistochemistry-in-102-samples-of-cervical-vulvar-anal-and-head-and-neck-neoplasia
#16
COMPARATIVE STUDY
Anne M Mills, Dawn C Dirks, Melinda D Poulter, Stacey E Mills, Mark H Stoler
Dysregulated expression of oncogenic types of E6 and E7 is necessary for human papillomavirus (HPV)-driven carcinogenesis. An HPV E6/E7 mRNA in situ hybridization (ISH) assay covering 18 common high-risk types ("HR-RISH," aka HR-HPV RNA18 ISH) has not been extensively studied in the anogenital tract or validated on automated technology. We herein compare HR-RISH to DNA polymerase chain reaction (PCR), p16 immunohistochemistry, and a previously available HPV DNA ISH assay in HPV-related anogenital and head and neck (H&N) neoplasia...
May 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28400857/estimation-of-the-overall-burden-of-cancers-precancerous-lesions-and-genital-warts-attributable-to-9-valent-hpv-vaccine-types-in-women-and-men-in-europe
#17
Susanne Hartwig, Jean Lacau St Guily, Géraldine Dominiak-Felden, Laia Alemany, Silvia de Sanjosé
BACKGROUND: In addition to cervical cancer, human papillomavirus (HPV) is responsible for a significant proportion of cancers and precancerous lesions of the vulva, vagina, anus, penis, head and neck, as well as genital warts. We estimated the annual number of new cases of these diseases attributable to 9-valent HPV vaccine types in women and men in Europe. METHODS: The annual number of new cancers of the cervix, vulva, vagina, anus, penis, and selected head and neck sites in the population of the European Medicines Agency territory was estimated based on age-specific incidence rates extracted from Cancer Incidence in 5 Continents, Volume X and Eurostat population data for 2015...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28397203/comparison-of-the-diagnostic-value-of-cervical-cytology-and-hpv-hr-dna-testing-for-the-diagnosis-of-low-grade-and-high-grade-squamous-intraepithelial-lesions-across-different-age-groups
#18
Aleksandra Paluszkiewicz, Dominik Pruski, Kinga Iwaniec, Witold Kędzia
OBJECTIVES: To assess the diagnostic value of cervical cytology and HPV HR DNA testing for the diagnosis of low grade and high-grade squamous intraepithelial lesions across different age groups. MATERIAL AND METHODS: The study included 1103 patients, age 25-70 years. All patients underwent in-depth diagnostic tests following either an abnormal Pap test result or a clinically suspicious cervical lesion. In all women the following examinations were performed: a molecular test detecting 14 high-risk types of HPV, a colposcopy examination, as well as directed-biopsy of the cervix...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28395466/-population-based-study-on-infection-and-genotype-distribution-of-high-risk-human-among-women-in-rural-areas-of-china-2014
#19
J L Di, X M Luo, J L Wu, B Song, L Ma
Objective: To explore the epidemiologic characterization of high-risk human papillomavirus (HR-HPV) infection and genotype distribution of HR-HPV among women in rural areas of China. Methods: This study used multiple layers of stratified cluster random sampling method. During January to December in 2014, 117 counties of 27 provinces were selected as the HPV test screening pilot project counties. The women aged 35-64 years with rural areas Hukou in these project counties were selected as the study subjects. A total 457 799 women received HPV DNA test...
April 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/28394582/multiplex-paper-based-colorimetric-dna-sensor-using-pyrrolidinyl-peptide-nucleic-acid-induced-agnps-aggregation-for-detecting-mers-cov-mtb-and-hpv-oligonucleotides
#20
Prinjaporn Tee-Ngam, Weena Siangproh, Adisorn Tuantranont, Tirayut Vilaivan, Orawon Chailapakul, Charles S Henry
The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, apaper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregationis reported as an alternative to traditional colorimetric approaches...
April 10, 2017: Analytical Chemistry
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