keyword
https://read.qxmd.com/read/38541090/the-role-of-%C3%AE-1-integrin-cd29-as-a-potential-prognostic-factor-for-the-risk-of-progression-to-cervical-carcinoma-in-hpv-associated-lesions
#1
JOURNAL ARTICLE
Maria Teresa Schettino, Eleonora Petra Preti, Valeria Vietri, Nadia Agrillo, Nicola Iavazzo, Diego Domenico Fasulo, Pasquale De Franciscis, Maria Rosaria Campitiello, Maria Giovanna Vastarella, Gaetano Riemma, Barbara Gardella, Filippo Murina
Background and Objectives : Available evidence reports the overexpression of β1 integrin in dysplastic rather than normal cervical tissue. We aimed to evaluate the involvement of β1 (CD29) integrin in the progressive pathogenesis of cervical intraepithelial neoplasia (CIN). Materials and Methods : From January 2019 to December 2021, we prospectively enrolled women undergoing a colposcopy with a cervical biopsy for abnormal cervical cytology and/or undefined cytology with a positive HPV DNA test and women with relapsing cervical inflammatory disorders...
February 21, 2024: Medicina
https://read.qxmd.com/read/38540340/sp1-gene-methylation-in-head-and-neck-squamous-cell-cancer-in-hpv-negative-patients
#2
JOURNAL ARTICLE
Enar Jumaniyazova, Anna Aghajanyan, Sergey Kurevlev, Leyla Tskhovrebova, Andrey Makarov, Konstantin Gordon, Anastasiya Lokhonina, Timur Fatkhudinov
There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of the study was to investigate the methylation status of CDKN1 , CDKN2A , MYC , Smad3 , SP1 , and UBC genes in tumor tissue (control-normal tissue) in 50 patients (37 men and 13 women) with HPV-negative HNSCC. Methods: Bisulfite conversion methods and methyl-sensitive analysis of high-resolution melting curves were used to quantify the methylation of genes...
February 23, 2024: Genes
https://read.qxmd.com/read/38539498/pretreatment-circulating-hpv16-dna-viral-load-predicts-risk-of-distant-metastasis-in-patients-with-hpv16-positive-oropharyngeal-cancer
#3
JOURNAL ARTICLE
Agnieszka Maria Mazurek, Iwona Jabłońska, Marek Kentnowski, Urszula Kacorzyk, Mirosław Śnietura, Tomasz Wojciech Rutkowski
BACKGROUND: There are definite reasons to implement molecular diagnostics based on the measurement of human papillomavirus (HPV) DNA in liquid biopsy into clinical practice. It is a quick, repeatable, and health-safe test for cancer biomarkers in the blood. In this study, we investigated whether the circulating tumor-related HPV16 (ctHPV16) viral load (VL) in patients with oropharyngeal squamous cell carcinoma (OPSCC) was important for determining the risk of locoregional recurrence-free survival (LRFS), metastasis-free survival (MFS), and overall survival (OS)...
March 15, 2024: Cancers
https://read.qxmd.com/read/38536624/validation-of-the-visionarray%C3%A2-chip-assay-for-hpv-dna-testing-in-histology-specimens-of-oropharyngeal-squamous-cell-carcinoma
#4
JOURNAL ARTICLE
Hani Ibrahim Channir, Simone Kloch Bendtsen, Linea Cecilie Melchior, Pia Rovsing Sandholm, Christine Mordhorst, Amanda-Louise Fenger Carlander, Christian von Buchwald, Katalin Kiss
BACKGROUND: The detection of human papillomavirus (HPV) has several implications in the diagnostic work-up and management of oropharyngeal squamous cell carcinoma (OPSCC). The choice of HPV detection assay and testing algorithms differ across institutions and vary in cost, detection targets, technical feasibility, and turnaround time. In this study, we aimed to validate the VisionArray® HPV Chip for formalin-fixed and paraffin-embedded (FFPE) samples of OPSCC using the previously applied standard pan-HPV DNA PCR assay as a reference...
March 27, 2024: Head and Neck Pathology
https://read.qxmd.com/read/38535031/assessment-of-cervicovaginal-smear-and-hpv-dna-co-test-for-cervical-cancer-screening-implications-for-diagnosis-and-follow-up-strategies
#5
JOURNAL ARTICLE
Neslihan Kaya Terzi, Ozden Yulek
OBJECTIVE: Cervical cancer is a major cause of cancer-related mortality, necessitating effective screening and diagnostic methods. This study aimed to assess the performance of cervicovaginal smear (CVS) and human papillomavirus (HPV)-DNA co-test. STUDY DESIGN: The pathology results of 225 female patients who underwent HPV-DNA testing with CVS between 2014 and 2022 and were subsequently diagnosed by colposcopic cervical biopsy or second CVS were retrospectively analyzed...
March 13, 2024: Diagnostics
https://read.qxmd.com/read/38534083/determinants-of-type-specific-human-papillomavirus-concordance-across-anatomic-sites-in-young-men-who-have-sex-with-men-and-transgender-women-3-u-s-cities-2016-2018
#6
JOURNAL ARTICLE
Adeel Shah, Elissa Meites, John Lin, James P Hughes, Pamina M Gorbach, Brian Mustanski, Richard A Crosby, Elizabeth R Unger, Troy Querec, Matthew Golden, Lauri E Markowitz, Rachel L Winer
BACKGROUND: Among men who have sex with men (MSM) and transgender women (TGW), the dynamics of human papillomavirus (HPV) infections at different anatomical sites are not well understood. Information on HPV concordance between anatomic sites can inform the extent of autoinoculation, and susceptibility of different anatomic areas to HPV infection. We described and assessed correlates of HPV concordance across anal, oral, and genital samples. METHODS: We enrolled 1876 MSM and TGW aged 18 to 26 years in 3 US cities...
April 1, 2024: Sexually Transmitted Diseases
https://read.qxmd.com/read/38530541/ginsenoside-rb1-deters-cell-proliferation-induces-apoptosis-alleviates-oxidative-stress-and-antimetastasis-in-oral-squamous-carcinoma-cells
#7
JOURNAL ARTICLE
Le An, Yang Yu, Long He, Xu Xiao, Pengcheng Li
There are numerous therapeutic applications for ginsenoside Rb1 (GRb1), the primary saponin derived from ginseng root. According to earlier research, ginsenoside Rb1 causes apoptosis and reduces the cell cycle. Its adverse effects, especially those on the development of the embryo, still need to be thoroughly studied. A host's lifestyle choices, including smoking, drinking too much alcohol, using tobacco products, and having an HPV infection, can increase the risk of oral squamous cell carcinoma (OSCC), one of the most prevalent malignancies of the oral cavity...
March 26, 2024: Applied Biochemistry and Biotechnology
https://read.qxmd.com/read/38528459/positivity-rates-trends-and-experiences-of-health-workers-on-human-papillomavirus-screened-using-genexpert-in-uganda-a-three-year-retrospective-cohort-study
#8
JOURNAL ARTICLE
Harriet Nakigozi, Paul Mukama Ategyeka, Susan Nabadda, Rawlance Ndejjo
INTRODUCTION: Cervical cancer is a major public health issue in Uganda, with high incidence due to limited screening especially in rural areas. In 2019, HPV DNA testing using GeneXpert was rolled out to improve screening access. Assessing progress and challenges since its introduction is important. AIM: To determine genotype distribution and explore health worker experiences with HPV screening using GeneXpert in Uganda. METHODS: We conducted a retrospective cohort study where HPV screening data from 66 GeneXpert labs from March 2021-May 2023 country wide was analyzed...
March 25, 2024: BMC Cancer
https://read.qxmd.com/read/38524273/dna-methylation-as-a-triage-tool-for-cervical-cancer-screening-a-meeting-report
#9
REVIEW
F Ricardo Burdier, Dur-E-Nayab Waheed, Belinda Nedjai, Renske D M Steenbergen, Mario Poljak, Marc Baay, Alex Vorsters, Severien Van Keer
INTRODUCTION: DNA methylation is proposed as a novel biomarker able to monitor molecular events in human papillomavirus (HPV) infection pathophysiology, enabling the distinction between HPV-induced lesions with regression potential from those that may progress to HPV-related cancer. METHODS: This meeting report summarises the presentations and expert discussions during the HPV Prevention and Control Board-focused topic technical meeting on DNA methylation validation in clinician-collected and self-collected samples, novel DNA methylation markers discovery, implementation in cervical cancer screening programs, and their potential in women living with human immunodeficiency virus (HIV)...
May 2024: Preventive Medicine Reports
https://read.qxmd.com/read/38523463/qualitative-and-quantitative-changes-in-mitochondrial-dna-associated-with-cervical-cancer-a-comprehensive-review
#10
REVIEW
Isadora Oliveira Ansaloni Pereira, Nayara Nascimento Toledo Silva, Angelica Alves Lima, Glenda Nicioli da Silva
Cervical cancer is the fourth most commonly diagnosed cancer in women and is considered a preventable disease, as vaccination and screening programs effectively reduce its incidence and mortality rates. Disease physiopathology and malignant cell transformation is a complex process, but it is widely known that high-risk HPV (hrHPV) infection is a necessary risk factor for cancer development. Mitochondria, cell organelles with important bioenergetic and biosynthetic functions, are important for cell energy production, cell growth, and apoptosis...
March 24, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38520625/plumbagin-as-a-preferential-lead-molecule-to-combat-egfr-driven-matrix-abundance-and-migration-of-cervical-carcinoma-cells
#11
JOURNAL ARTICLE
Sneha Krishnamoorthy, Rajalakshmi Sabanayagam, Loganayaki Periyasamy, Bharathi Muruganantham, Sridhar Muthusami
The handshake between the complex networks of matrix components in the tumor micro-environment (TME) is considered as a crucial event in the progression of several cancers including cervical carcinoma (CC). A number of studies report a connection between epidermal growth factor (EGF) and matrix component production. Studies demonstrate that the mechano-transduction trigger by collagen, influences the tumor cells to undergo epithelial-mesenchymal transition (EMT) and block the entry of drugs. We hypothesize that the intervention to prevent EGF triggered deposition of matrix components could sensitize several therapies for CC cells...
March 23, 2024: Medical Oncology
https://read.qxmd.com/read/38516891/ctdna-transiting-into-urine-is-ultrashort-and-facilitates-noninvasive-liquid-biopsy-of-hpv-oropharyngeal-cancer
#12
JOURNAL ARTICLE
Chandan Bhambhani, Qing Kang, Daniel H Hovelson, Erin Sandford, Mary Olesnavich, Sarah M Dermody, Jenny Wolfgang, Kirsten L Tuck, Collin Brummel, Apurva D Bhangale, Kuang He, Marc G Gutierrez, Ryan H Lindstrom, Chia-Jen Liu, Melissa Tuck, Malathi Kandarpa, Michelle Mierzwa, Keith Casper, Mark E Prince, John C Krauss, Moshe Talpaz, N Lynn Henry, Maria D Giraldez, Nithya Ramnath, Scott A Tomlins, Paul L Swiecicki, J Chad Brenner, Muneesh Tewari
BACKGROUNDTransrenal cell-free tumor DNA (TR-ctDNA), which transits from the bloodstream into urine, has the potential to enable noninvasive cancer detection for a wide variety of nonurologic cancer types.MethodsUsing whole-genome sequencing, we discovered that urine TR-ctDNA fragments across multiple cancer types are predominantly ultrashort (<50 bp) and, therefore, likely to be missed by conventional ctDNA assays. We developed an ultrashort droplet digital PCR assay to detect TR-ctDNA originating from HPV-associated oropharyngeal squamous cell carcinoma (HPV+ OPSCC) and confirmed that assaying ultrashort DNA is critical for sensitive cancer detection from urine samples...
February 8, 2024: JCI Insight
https://read.qxmd.com/read/38516796/intrinsic-rna-targeting-triggers-indiscriminate-dnase-activity-of-crispr-cas12a
#13
JOURNAL ARTICLE
Jiongyu Zhang, Ziyue Li, Chong Guo, Xin Guan, Lori Avery, David Banach, Changchun Liu
The CRISPR-Cas12a system has emerged as a powerful tool for nucleic acid-based molecular diagnostics. However, it has long been believed to be effective only on DNA targets. Here, we investigate the intrinsic RNA-enabled trans-cleavage activity of AsCas12a and LbCas12a and discover that they can be directly activated by fully complementary RNA targets, although LbCas12a exhibits weaker trans-cleavage activity than AsCas12a on single-stranded DNA and RNA substrates. Remarkably, we find that the RNA-activated Cas12a exhibits higher specificity compared to DNA activation...
March 22, 2024: Angewandte Chemie
https://read.qxmd.com/read/38513185/human-papillomavirus-attributable-head-and-neck-cancers-in-india-a-systematic-review-and-meta-analysis
#14
REVIEW
Nandimandalam Venkata Vani, Ranganathan Rama, Rajendran Madhanagopal, Ramshankar Vijayalakshmi, Rajaraman Swaminathan
PURPOSE: Head and neck cancer accounts for about one third of the global burden in India. Mucosal high-risk human papillomavirus (HPV) has been hypothesized as a contributory risk factor for head and neck cancer (HNC) but its prevalence in Indian patients is not well established. Therefore, this systematic review and meta-analysis aimed to estimate the prevalence of HPV in HNC in India and their attributable fraction by considering the biomarkers of carcinogenesis, p16, and HPV E6/E7 mRNA...
March 2024: JCO global oncology
https://read.qxmd.com/read/38507581/long-term-prediction-by-dna-methylation-of-high-grade-cervical-intraepithelial-neoplasia-results-of-the-artistic-cohort
#15
JOURNAL ARTICLE
Clare Gilham, Belinda Nedjai, Dorota Scibior-Bentkowska, Caroline Reuter, Rawinder Banwait, Adam R Brentnall, Jack Cuzick, Julian Peto, Attila T Lorincz
Methylation markers have shown potential for triaging high-risk HPV-positive (hrHPV+) women to identify those at increased risk of invasive cervical cancer (ICC). Our aim was to assess the performance of the S5 DNA methylation classifier for predicting incident high-grade cervical intraepithelial neoplasia (CIN) and ICC among hrHPV+ women in the ARTISTIC screening trial cohort. The S5 classifier, comprising target regions of tumour suppressor gene EPB41L3 and L1 and L2 regions of HPV16, HPV18, HPV31, and HPV33, was assayed by pyrosequencing in archived hrHPV+ liquid-based samples from 343 women with high-grade disease (139 CIN2, 186 CIN3, and 18 ICC) compared to 800 hrHPV+ controls...
March 20, 2024: International Journal of Cancer. Journal International du Cancer
https://read.qxmd.com/read/38507429/high-rate-of-high-risk-human-papillomavirus-among-benign-and-breast-cancer-patients-in-ethiopia
#16
JOURNAL ARTICLE
Esmael Besufikad Belachew, Adey Feleke Desta, Andargachewu Mulu, Dinikisira Bekele Deneke, Dessalegn Abeje Tefera, Ashenafi Alemu, Endale Anberber, Daniel Beshah, Selfu Girma, Dareskedar Tsehay Sewasew, Tesfaye Sisay Tessema, Rawleigh Howe
INTRODUCTION: There have been numerous studies that showed the presence of human papillomavirus (HPV) in breast cancer; nonetheless, there is ongoing debate regarding their association. Given few studies in Ethiopia, we aimed to investigate the magnitude of HPV infection in Ethiopian breast cancer patients. METHODS: A total of 120 formalin-fixed paraffin-embedded (FFPE) tissue blocks were obtained, and basic demographic, clinical, and histological data were collected from medical records...
2024: PloS One
https://read.qxmd.com/read/38506408/advances-in-testing-for-human-papillomavirus-mediated-head-and-neck-cancer
#17
JOURNAL ARTICLE
Hisham Mehanna, Laia Alemany, Christian von Buchwald
PURPOSE OF REVIEW: New evidence has recently emerged regarding the utility and benefits of dual p16INKa (p16) and Human papillomavirus (HPV) status testing when determining the diagnosis and prognosis of patients with oropharyngeal cancer. RECENT FINDINGS: HPV RNA polymerase chain reaction (PCR) is the most accurate diagnostic test. The other assays (HPV DNA PCR, HPV DNA/RNA in-situ hybridization (ISH) and p16) applied to formalin fixed tumour tissue have varying but high sensitivities and specificities...
March 20, 2024: Current Opinion in Oncology
https://read.qxmd.com/read/38505587/clinical-and-genomic-characterization-of-chemoradiation-resistant-hpv-positive-oropharyngeal-squamous-cell-carcinoma
#18
JOURNAL ARTICLE
Theresa Guo, Fernando Zamuner, Stephanie Ting, Liam Chen, Lisa Rooper, Pablo Tamayo, Carole Fakhry, Daria Gaykalova, Ranee Mehra
INTRODUCTION: Most patients with HPV-positive oropharyngeal squamous cell carcinoma (OPSCC) have an excellent response to chemoradiation, and trials are now investigating de-escalated treatment. However, up to 25% of patients with HPV-positive OPSCC will experience recurrence, and up to 5% will even progress through primary treatment. Currently, there are no molecular markers to identify patients with poor prognosis who would be harmed by de-escalation. Herein we report the clinical and genomic characteristics of persistent HPV-positive OPSCC after definitive platinum-based chemoradiation therapy...
2024: Frontiers in Oncology
https://read.qxmd.com/read/38498999/the-role-of-neutrophil-lymphocytes-ratio-nlr-in-the-prognosis-of-cin2-2-recurrence-after-excisional-treatment
#19
JOURNAL ARTICLE
Mattia Dominoni, Amelia Barcellini, Marianna Francesca Pasquali, Annalisa De Silvestri, Virginia Valeria Ferretti, Stefania Cesari, Giacomo Fiandrino, Ester Orlandi, Barbara Gardella
The main risk factor involved in CIN2+ recurrence after treatment is the HPV persistent infection. The dysregulation of the immune system permits only HR-HPVs to become persistent infections, to promote cancer development and to increase the risk of recurrence after treatment. Therefore, there is a shift to a Th2-type cytokine pattern during the carcinogenesis pathway; for this reason, the neutrophil-lymphocytes ratio (NLR) could be a marker of this immunological change. The study aims to analyse the predictive role of NLR in the recurrence of High-grade CIN (CIN2+) after excisional treatment in a real-world life setting of patients treated for CIN2+ Design: cross-sectional study Participants/Materials, Setting, Methods: We examined a retrospective database of 444 patients, who attended the Colposcopy Service of our Department from 2011 to 2020 due to an abnormal screening pap smear and we compared the clinical characteristics to NLR performed at the time of diagnosis...
March 18, 2024: Gynecologic and Obstetric Investigation
https://read.qxmd.com/read/38494078/evaluation-of-pre-analytical-variables-for-hpv-primary-screening-from-self-collected-vaginal-swabs
#20
JOURNAL ARTICLE
Michelle Qi, Anissa R Naranjo, Abigail J Duque, Thomas S Lorey, Jeffrey M Schapiro, Betty J Suh-Burgmann, Michael Rummel, Stephen J Salipante, Nicolas Wentzensen, Dina N Greene
HPV primary screening is an effective approach to assessing cervical cancer risk. Self-collected vaginal swabs have shown promise to expand testing access, but there are limited data defining analytical performance criteria necessary for adoption of self-collected specimens, especially for those occurring outside the clinic where the swab remains dry during transport. Here, we evaluated the performance of self-collected vaginal swabs for HPV detection using the Roche Cobas 6800. There was insignificant variability between swabs self-collected by the same individual (n=15 participants collecting 5 swabs/participant), measured by amplification of HPV and human β-globin control DNA...
March 15, 2024: Journal of Molecular Diagnostics: JMD
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