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https://www.readbyqxmd.com/read/28722212/active-human-papillomavirus-involvement-in-barrett-s-dysplasia-and-oesophageal-adenocarcinoma-is-characterized-by-wild-type-p53-and-aberrations-of-the-retinoblastoma-protein-pathway
#1
Shanmugarajah Rajendra, Tao Yang, Wei Xuan, Prateek Sharma, Darren Pavey, Cheong Soon Lee, Son Le, Josephine Collins, Bin Wang
We have previously demonstrated that transcriptionally active high-risk HPV (hr-HPV) is strongly incriminated in Barrett's dysplasia (BD) and oesophageal adenocarcinoma (OAC) using mainly fresh frozen tissue. This study aimed to identify biomarkers of active HPV infection in Barrett's metaplasia, (BM)/BD/OAC by immunohistochemical staining (IHC) of formalin-fixed paraffin embedded (FFPE) tissue for aberrations of p53 and the retinoblastoma (pRb) pathway which are targets for the viral oncoproteins, E6/E7 respectively...
July 19, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28720459/investigating-the-role-of-human-papillomavirus-in-lung-cancer
#2
E Argyri, E Tsimplaki, C Marketos, G Politis, E Panotopoulou
Lung cancer is the leading cancer worldwide among men and women with morbidity reaching 1.6 million. Human Papillomavirus is the causal factor of cervical cancer while its association with others is still under investigation. The purpose of our study is to examine the presence of HPV DNA as well as high-risk E6/E7 mRNA in patients with lung cancer. Lung tissues were collected during bronchoscopy and tested for HPV DNA and E6/E7 mRNA. 67 lung tissue samples were analysed. The age range was 49-85 years old (y...
June 2017: Papillomavirus Research
https://www.readbyqxmd.com/read/28717857/concordance-of-hpv-load-and-hpv-mrna-for-16-carcinogenic-possibly-carcinogenic-hpv-types-in-paired-smear-tissue-cervical-cancer-specimens
#3
Gordana Halec, Bolormaa Dondog, Michael Pawlita, Markus Schmitt
HPV types with high viral load are associated with cervical abnormalities. However, viral load measurements and concordance of HPV loads and viral mRNA have not been demonstrated for all high-risk/possibly high-risk (HR-/pHR-)HPV types in cervical cancer (CxCa). Especially, the biological role of co-infecting HR-/pHR-HPV types with low viral load has not been thoroughly investigated. Using BSGP5+/6+-PCR/MPG genotyping, we analyzed viral loads for all currently defined 51 mucosal HPV types in 74 cervical smears from patients with CxCa and compared this data with HPV DNA and mRNA status in these patients' corresponding CxCa tissues...
July 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28717061/apobec-mediated-genomic-alterations-link-immunity-and-viral-infection-during-human-papillomavirus-driven-cervical-carcinogenesis
#4
Lanting Chen, Xuemin Qiu, Na Zhang, Yan Wang, Mingyan Wang, Dajin Li, Ling Wang, Yan Du
Cervical cancer is one of the most frequently diagnosed cancers and is a major cause of death from gynecologic cancers worldwide; the cancer burden from cervical cancer is especially heavy in less developed countries. Most cases of cervical cancer are caused by persistent infection with carcinogenic human papillomavirus (HPV) genotypes 16 and 18. Non-resolving inflammation caused by HPV infection provides a microenvironment that facilitates cancer development. Molecular alterations during the process of HPV-induced carcinogenesis are characterized by DNA methylation within the HPV genome, promoter hypermethylation of tumor suppressor genes in the host genome, as well as genomic instability caused by viral DNA integrating into the host genome...
July 17, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28714529/immunologic-responses-to-a-novel-dna-vaccine-targeting-human-papillomavirus-11-e6e7
#5
Julie Ahn, Shiwen Peng, Chien-Fu Hung, Richard B S Roden, Tzyy-Choou Wu, Simon R Best
OBJECTIVES/HYPOTHESIS: Recurrent respiratory papillomatosis (RRP) is a benign disease caused by human papillomavirus (HPV) types 6 and 11. Although a prophylactic vaccine against RRP is available, a therapeutic vaccine is needed to treat those already infected. The objective of our study was to design and test a DNA vaccine targeting HPV11 proteins. STUDY DESIGN: Preclinical scientific investigation. METHODS: A DNA vaccine encoding the HPV11 E6 and E7 genes linked to calreticulin (CRT) was generated...
July 17, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28713770/prognostic-impact-of-ajcc-uicc-8th-edition-new-staging-rules-in-oropharyngeal-squamous-cell-carcinoma
#6
Nora Würdemann, Steffen Wagner, Shachi Jenny Sharma, Elena-Sophie Prigge, Miriam Reuschenbach, Stefan Gattenlöhner, Jens Peter Klussmann, Claus Wittekindt
INTRODUCTION: The purpose of this study was to test whether the 8th edition of the AJCC/UICC TNM staging system (UICC) precisely differentiates between stages and reflects disease outcome in human papilloma virus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCC). PATIENTS AND METHODS: OPSCC patients that were diagnosed between 2000 and 2016 were included in this analysis and HPV status was determined by combined DNA and p16 testing. Stratification was done according to 7th and 8th UICC staging rules...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28710863/assessment-of-the-effectiveness-of-hpv16-18-infection-referred-for-colposcopy-in-cervical-cancer-screening-in-northwest-of-china
#7
Qian Zhang, Minyi Zhao, Di Cao, Xing Wei, Li Wang, Yang Li, Ting Yang, Juan Zhao, Meili Pei, Hongran Jia, Siyu Cao, Shimin Quan, Xiaofeng Yang
OBJECTIVE: To evaluate the effectiveness of HPV16/18 infection referral to colposcopy in cervical cancer screening for women aged 25 years and older in Chinese northwest region Shaan'xi province. METHODS: 2224 women were diagnosed with primary high-risk HPV infection by HPV-DNA genotyping technology during August 2014 to August 2015. 1916 cases referred for colposcopy with histological evidence were enrolled, including 1124 women with HPV16/18 genotype and 792 with other HR-HPV genotypes...
July 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28705719/prevalence-of-ebv-hpv-and-mmtv-in-pakistani-breast-cancer-patients-a-possible-etiological-role-of-viruses-in-breast-cancer
#8
Wasifa Naushad, Orooj Surriya, Hajra Sadia
BACKGROUND: Breast cancer being a multifactorial disease, the role of infectious agent in development of disease is of great interest. The high incidence of breast cancer around the world has woken the interest in a viral etiology of breast cancer. Despite decades of research, no etiologic factor(s) for human breast cancer has been known and the quest for a contributing cause has all but been abandoned during the past years. Recent investigations have linked breast cancer to viral infections, such as Epstein-Barr virus (EBV), Human papillomavirus (HPV) and mouse mammary tumor virus...
July 10, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28704717/implementing-community-based-cervical-cancer-screening-programs-using-visual-inspection-with-acetic-acid-in-india-a-systematic-review
#9
REVIEW
Prajakta Adsul, Nitin Manjunath, Vijaya Srinivas, Anjali Arun, Purnima Madhivanan
The objective of this review was to systematically appraise the existing published literature about community-based cervical cancer screening programs that have used visual inspection methods using acetic acid (VIA) in India. All peer reviewed journal articles till December 2015 were searched per PRISMA guidelines. Articles reporting results from cervical cancer screening programs in community-based settings, conducted in India, and using VIA were included in this review. The search resulted in 20 articles to be included in the review with a total of 313,553 women at 12 unique urban and rural sites across India...
July 10, 2017: Cancer Epidemiology
https://www.readbyqxmd.com/read/28704213/menstrual-pad-a-cervical-cancer-screening-tool-a-population-based-study-in-rural-india
#10
Atul Budukh, Vrushali Palayekar, Amita Maheshwari, Kedar Deodhar, Pallavi Purwar, Sonali Bagal, Ashok Vadigoppula, Manoj Lokhande, Nandkumar Panse, Rajesh Dikshit, Rajendra Badwe
In the rural areas of India, women generally use a piece of old cloth as a menstrual device. The aim of this study was to detect human papilloma virus (HPV) from menstrual blood on the menstrual pad and assess whether this could be a useful screening tool for cervical cancer. In Jamkhed area of rural Maharashtra, (population A), we collected menstrual pads from women who provided consent in the 30-50 year age group. The women who had provided menstrual pads underwent HC2 testing. We standardized the method for extracting DNA by PCR from the menstrual pad...
July 12, 2017: European Journal of Cancer Prevention
https://www.readbyqxmd.com/read/28701400/whole-genome-sequencing-and-variant-analysis-of-hpv16-infections
#11
Pascal van der Weele, Chris J L M Meijer, Audrey J King
Human papillomavirus (HPV) is a strongly conserved DNA virus, of which high-risk types can cause cervical cancer in persistent infections. The most common type found in HPV-attributable cancer is HPV16, which can be subdivided into four lineages (A-D) with different carcinogenic properties. Studies have shown HPV16 sequence diversity in different geographical areas, but only limited information is available regarding HPV16 diversity within a population, especially at the whole genome level. We have analyzed HPV16 major variant diversity, conservation in persistent infections and performed a SNP comparison between persistent and clearing infections...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28700089/prevalence-and-genotype-distribution-of-human-papillomavirus-among-females-in-the-suburb-of-shanghai-china
#12
Chengzhong Zhang, Chao Zhang, Jie Huang, Zhouwei Wu, Xingyu Mei, Weimin Shi
OBJECTIVES: To describe the prevalence of human papillomavirus (HPV) and its genotype distribution among females in the suburb of Shanghai. METHODS: A total of 33,562 participants were enrolled in this study from January to December 2016. HPV GenoArray test kit was used to perform HPV genotyping and was also used in DNA amplification and HybriBio's proprietary flow-through hybridization technique. RESULTS: The overall prevalence of HPV was 18...
July 12, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28700080/anal-hpv-16-and-18-viral-load-a-comparison-between-hiv-negative-and-hiv-positive-msm-and-association-with-persistence
#13
Elske Marra, Audrey King, Elske van Logchem, Pascal van der Weele, Sofie H Mooij, Titia Heijman, Chris J L M Meijer, Dominique W M Verhagen, Marianne A B van der Sande, Maarten F Schim van der Loeff
Objective Does anal HPV viral load explain the difference in anal HPV persistence between HIV-negative and HIV-positive men who have sex with men (MSM)? Methods MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010-2011. Anal self-swabs were collected every 6 months and genotyped (SPF10 -PCR-DEIA-LIPA25 -system). HPV16 and HPV18 load was determined with a type specific quantitative (q)PCR, and compared between HIV-negative and HIV-positive men using ranksum test. Persistence was defined as ≥3 positive samples for the same HPV-type...
July 12, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28699189/human-papillomavirus-infects-placental-trophoblast-and-hofbauer-cells-but-appears-not-to-play-a-causal-role-in-miscarriage-and-preterm-labor
#14
Lea Maria Margareta Ambühl, Anne Katrine Leonhard, Carina Widen Zakhary, Annemette Jørgensen, Jan Blaakaer, Karen Dybkaer, Ulrik Baandrup, Niels Uldbjerg, Suzette Sørensen
INTRODUCTION: Recently, an association between human papillomavirus (HPV) infection and both spontaneous abortion and spontaneous preterm delivery was suggested. However, the reported HPV prevalence in pregnant women varies considerably and reliable conclusions are difficult. We aimed to investigate HPV infection in placental tissue of a Danish study cohort. Furthermore, we studied the cellular localization of HPV. MATERIAL AND METHODS: In this prospective case-control study, placental tissue was analyzed for HPV infection by nested PCR in the following four study groups: full-term delivery (n=103), spontaneous preterm delivery (n=69), elective abortion (n=54), and spontaneous abortion (n=44)...
July 12, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28698524/telomerase-induction-in-hpv-infection-and-oncogenesis
#15
REVIEW
Rachel Katzenellenbogen
Telomerase extends the repetitive DNA at the ends of linear chromosomes, and it is normally active in stem cells. When expressed in somatic diploid cells, it can lead to cellular immortalization. Human papillomaviruses (HPVs) are associated with and high-risk for cancer activate telomerase through the catalytic subunit of telomerase, human telomerase reverse transcriptase (hTERT). The expression of hTERT is affected by both high-risk HPVs, E6 and E7. Seminal studies over the last two decades have identified the transcriptional, epigenetic, and post-transcriptional roles high-risk E6 and E7 have in telomerase induction...
July 10, 2017: Viruses
https://www.readbyqxmd.com/read/28696386/analytic-and-diagnostic-performances-of-human-papillomavirus-e6-e7-mrna-test-on-up-to-11-year-old-liquid-based-cervical-samples-a-biobank-based-longitudinal-study
#16
Roberta Zappacosta, Francesca Sablone, Lucia Pansa, Davide Buca, Danilo Buca, Sandra Rosini
Human Papillomavirus (HPV) E6/E7 mRNA test demonstrated high specificity in detecting HPV infections, but studies assessing its efficacy in terms of cancer risk stratification are lacking. Follow-up studies are arduous and expensive. Biobank would be the answer to the problem, although data investigating the effects of long-term storage on RNA preservation are still needed. We addressed these issues by retrieving 202 residual liquid-based cervical specimens, collected from 149 women attending cervical cancer screening during the years 2001-2012...
July 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28694699/antitumor-hpv-e7-specific-ctl-activity-elicited-by-in-vivo-engineered-exosomes-produced-through-dna-inoculation
#17
Paola Di Bonito, Chiara Chiozzini, Claudia Arenaccio, Simona Anticoli, Francesco Manfredi, Eleonora Olivetta, Flavia Ferrantelli, Emiliana Falcone, Anna Ruggieri, Maurizio Federico
We recently proved that exosomes engineered in vitro to deliver high amounts of HPV E7 upon fusion with the Nef(mut) exosome-anchoring protein elicit an efficient anti-E7 cytotoxic T lymphocyte immune response. However, in view of a potential clinic application of this finding, our exosome-based immunization strategy was faced with possible technical difficulties including industrial manufacturing, cost of production, and storage. To overcome these hurdles, we designed an as yet unproven exosome-based immunization strategy relying on delivery by intramuscular inoculation of a DNA vector expressing Nef(mut) fused with HPV E7...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28692681/cervical-hpv-type-specific-pre-vaccination-prevalence-and-age-distribution-in-croatia
#18
Ivan Sabol, Nina Milutin Gašperov, Mihaela Matovina, Ksenija Božinović, Goran Grubišić, Ivan Fistonić, Dragan Belci, Laia Alemany, Sonja Džebro, Mara Dominis, Mario Šekerija, Sara Tous, Silvia de Sanjosé, Magdalena Grce
The main etiological factor of precancerous lesion and invasive cervical cancer are oncogenic human papillomaviruses types (HPVs). The objective of this study was to establish the distribution of the most common HPVs in different cervical lesions and cancer prior to the implementation of organized population-based cervical screening and HPV vaccination in Croatia. In this study, 4,432 cervical specimens, collected through a 16-year period, were tested for the presence of HPV-DNA by polymerase chain reaction (PCR) with three sets of broad-spectrum primers and type-specific primers for most common low-risk (LR) types (HPV-6, 11) and the most common high-risk (HR) types (HPV-16, 18, 31, 33, 45, 52, 58)...
2017: PloS One
https://www.readbyqxmd.com/read/28691334/the-health-and-economic-impact-of-scaling-cervical-cancer-prevention-in-50-low-and-lower-middle-income-countries
#19
Nicole G Campos, Monisha Sharma, Andrew Clark, Kyueun Lee, Fangli Geng, Catherine Regan, Jane Kim, Stephen Resch
OBJECTIVE: To estimate the health impact, financial costs, and cost-effectiveness of scaling-up coverage of human papillomavirus (HPV) vaccination (young girls) and cervical cancer screening (women of screening age) for women in countries that will likely need donor assistance. METHODS: We used a model-based approach to synthesize population, demographic, and epidemiological data from 50 low- and lower-middle-income countries. Models were used to project the costs (US $), lifetime health impact (cervical cancer cases, deaths averted), and cost-effectiveness (US $ per disability adjusted life year [DALY] averted) of: (1) two-dose HPV-16/18 vaccination of girls aged 10 years; (2) once-in-a-lifetime screening, with treatment when needed, of women aged 35 years with either HPV DNA testing or visual inspection with acetic acid (VIA); and (3) cervical cancer treatment over a 10-year roll-out...
July 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28688605/human-papillomavirus-prevalence-in-the-anus-and-urine-among-hiv-infected-japanese-men-who-have-sex-with-men
#20
Hiroshi Yaegashi, Kazuyoshi Shigehara, Ichiro Itoda, Mitsuaki Ohkodo, Kazufumi Nakashima, Shohei Kawaguchi, Mikio Ueda, Koji Izumi, Yoshifumi Kadono, Hiroko Ikeda, Mikio Namiki, Atsushi Mizokami
BACKGROUND: The present study investigated human papillomavirus (HPV) prevalence in anal and urine samples, and evaluated cytological findings among human immunodeficiency virus (HIV)-infected Japanese men who have sex with men (MSM). METHODS: A total of 148 patients were enrolled. Anal and urine samples were collected from each participant, and a HPV-DNA test and genotyping were performed using flow-through hybridization. In addition, anal cytology was evaluated based on Papanicolaou staining...
July 6, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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