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https://www.readbyqxmd.com/read/28330323/modification-of-sargassum-angustifolium-by-molybdate-during-a-facile-cultivation-for-high-rate-phosphate-removal-from-wastewater-structural-characterization-and-adsorptive-behavior
#1
Firozeh Saberzadeh Sarvestani, Hossein Esmaeili, Bahman Ramavandi
In this paper, a new and facile approach for molybdate loading in the brown algae of Sargassum angustifolium is introduced. The molybdate ions were entered into the algae body during a short cultivation to produce algae-Mo as a novel adsorbent for eliminating phosphate ions from synthetic and real wastewaters. Results of the surface analysis showed that molybdate loading onto the algae was successfully performed. Herein, basic variables, such as initial solution pH, adsorbent dosage, contact time, phosphate concentration, and temperature, were investigated in detail to assess the phosphate adsorption performance of algae-Mo...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330317/molecular-detection-and-pathogenicity-of-a-nucleopolyhedrovirus-isolated-from-looper-caterpillar-hyposidra-talaca-a-tea-pest
#2
Soma Dasgupta, Hijam Ranjit Singh, Sudripta Das, Sunil Kumar Pathak, Rakesh Kumar Bhola
Hyposidra talaca is a major defoliating pest of tea plants in north-eastern part of India. In this study, we look for variations (if any) in the attacking virus. Viral samples were collected from different regions of the northern part of West Bengal in India and were analyzed by PCR technique to study the variations across the region. The partial segment of the HytaNPV polyhedrin gene was cloned and sequenced. A 527 bp nucleotide sequence containing highly conserved region from polyhedrin gene of HytaNPV was observed...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330164/therapeutic-potential-of-cyanobacteria-against-streptozotocin-induced-diabetic-rats
#3
Muthuraman Pandurangan, Doo Hwan Kim
Hypoglycemic effect of cyanobacteria has evaluated in the normal and streptozotocin-induced diabetic male albino rats as a mammalian model. Normal and streptozotocin-diabetic rats were orally administered cyanobacteria for 60 consecutive days, and their blood levels of glucose, insulin, C-peptide, lipid peroxidation, body weight and enzyme changes were determined using standard methods. Cyanobacteria administration reduced blood glucose level, and increased plasma insulin, C-peptide levels and restored the body weight...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330118/cloning-expression-purification-and-characterization-of-lipase-from-bacillus-licheniformis-isolated-from-hot-spring-of-himachal-pradesh-india
#4
Gagandeep Kaur, Amninder Singh, Rohit Sharma, Vinay Sharma, Swati Verma, Pushpender K Sharma
In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusion bodies demonstrated ~22 kDa protein band on 12 % SDS-PAGE. It exhibited specific activity of 0.49 U mg(-1) and % yield of 8.58. Interestingly, the lipase displayed activity at wide range of pH and temperature, i...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330113/protein-fusion-tags-for-efficient-expression-and-purification-of-recombinant-proteins-in-the-periplasmic-space-of-e-coli
#5
REVIEW
Ajamaluddin Malik
Disulfide bonds occurred in majority of secreted protein. Formation of correct disulfide bonds are must for achieving native conformation, solubility and activity. Production of recombinant proteins containing disulfide bond for therapeutic, diagnostic and various other purposes is a challenging task of research. Production of such proteins in the reducing cytosolic compartment of E. coli usually ends up in inclusion bodies formation. Refolding of inclusion bodies can be difficult, time and labor consuming and uneconomical...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#6
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329885/unexpected-intracameral-foreign-body-during-routine-cataract-surgery
#7
L Eggenschwiler, S Savic Prince, L Bubendorf, C Walder, D Goldblum
No abstract text is available yet for this article.
March 22, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28329858/cystatin-c-and-the-risk-of-frailty-and-mortality-in-older-men
#8
Allyson Hart, Terri L Blackwell, Misti L Paudel, Brent C Taylor, Eric S Orwoll, Peggy M Cawthon, Kristine E Ensrud
Background: This study examines the association between cystatin C (cysC) levels and risks of progression of frailty status or death in older men. Methods: Prospective study of 2,613 men without overt frailty aged 67 years and older enrolled in the MrOS ancillary sleep study. Baseline measurements included serum cysC, serum creatinine, and frailty status. Repeat frailty status, performed an average of 3.4 years later, was assessed as an ordinal outcome of robust, intermediate stage (prefrail), frail or dead...
November 9, 2016: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28329857/-play-and-people-living-with-dementia-a-humanities-based-inquiry-of-timeslips-and-the-alzheimer-s-poetry-project
#9
Aagje Swinnen, Kate de Medeiros
This paper is a humanities-based inquiry, applying Huizinga's framework of homo ludens ("man the player") to consider "play" in the context of two participatory arts programs (TimeSlips and the Alzheimer's Poetry Project) for people living with dementia. "Play," according to this Dutch historian, is at the heart of human activity and what gives meaning to life. Despite empirical research on play across the life course, play in dementia care is a relatively new idea. In addition, there is a dearth of reports based on humanistic inquiry which has slightly different goals than the growing body of qualitative and quantitative studies of participatory arts interventions...
January 18, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329827/the-influence-of-the-physical-envirnment-on-residents-with-dementia-in-long-term-care-settings-a-review-of-the-empirical-literature
#10
Habib Chaudhury, Heather A Cooke, Heather Cowie, Leila Razaghi
Background and Objectives: The physical environment in long-term care facilities has an important role in the care of residents with dementia. This paper presents a literature review focusing on recent empirical research in this area and situates the research with therapeutic goals related to the physical environment. Research Design and Methods: A comprehensive literature search was conducted in Ageline, PsychINFO, CINAHL, Medline and Google Scholar databases to identify relevant articles...
March 10, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329787/circulating-estrogen-levels-and-self-reported-health-and-mobility-limitation-in-community-dwelling-men-of-the-framingham-heart-study
#11
Guneet Kaur Jasuja, Thomas G Travison, Joanne M Murabito, Maithili N Davda, Adam J Rose, Shehzad Basaria, Andrea Coviello, Ramachandran S Vasan, Ralph D'Agostino, Shalender Bhasin
Background: Self-rated health is a commonly used global indicator of health status. Few studies have examined the association of self-rated health and mobility with estrone and estradiol in men. Accordingly, we determined the cross-sectional, incident, and mediating relations between circulating estrone and estradiol levels with self-rated health, mobility limitation, and physical performance in community-dwelling men. Methods: The cross-sectional sample included 1,148 men, who attended Framingham Offspring Study Examinations 7 and 8...
October 24, 2016: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28329752/prevalence-and-possible-risk-factors-for-urinary-incontinence-a-cohort-study-in-the-city-of-izmir
#12
Omer Demir, Volkan Sen, Bora Irer, Ozan Bozkurt, Adil Esen
INTRODUCTION: This study aimed to evaluate the prevalence and possible risk factors of urinary incontinence (UI) among women aged 18 or older living in the city of Izmir, located in the Aegean coast of Turkey. METHODS: A questionnaire and the validated International Consultation on Incontinence Questionnaire-Short Form (ICIQ-SF) were filled by urologists (O.D., V.S., A.E., O.B., and B.I.) with face-to-face interview. The participants with urge UI (UUI) and stress UI (SUI) were compared in terms of risk factors, quality of life (QoL) scores, severity of incontinence, status of doctor consultation, and total ICIQ-SF scores...
March 22, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28329716/-i-ll-do-anything-to-maintain-my-health-how-women-aged-65-94-perceive-experience-and-cope-with-their-aging-bodies
#13
Erica V Bennett, Laura Hurd Clarke, Kent C Kowalski, Peter R E Crocker
We explored how physically active women perceived, experienced, and coped with their aging bodies, and examined their perceptions of the utility of self-compassion to manage aging body-related changes. Findings from a thematic analysis of interviews with 21 women aged 65-94 revealed that they were appreciative of how their bodies worked and accepting of their physical limitations, yet concurrently critical of their body's functionality and appearance. Participants engaged in physical activity and healthy eating to maintain their health and body functionality, yet also used diet, hair styling, anti-aging creams, makeup, physical activity, and clothing to manage their appearances...
March 19, 2017: Body Image
https://www.readbyqxmd.com/read/28329686/demethylated-hsatii-dna-and-hsatii-rna-foci-sequester-prc1-and-mecp2-into-cancer-specific-nuclear-bodies
#14
Lisa L Hall, Meg Byron, Dawn M Carone, Troy W Whitfield, Gayle P Pouliot, Andrew Fischer, Peter Jones, Jeanne B Lawrence
This study reveals that high-copy satellite II (HSATII) sequences in the human genome can bind and impact distribution of chromatin regulatory proteins and that this goes awry in cancer. In many cancers, master regulatory proteins form two types of cancer-specific nuclear bodies, caused by locus-specific deregulation of HSATII. DNA demethylation at the 1q12 mega-satellite, common in cancer, causes PRC1 aggregation into prominent Cancer-Associated Polycomb (CAP) bodies. These loci remain silent, whereas HSATII loci with reduced PRC1 become derepressed, reflecting imbalanced distribution of UbH2A on these and other PcG-regulated loci...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28329672/vitamin-d-and-skin-aging
#15
Merel A Hamer, Raymond Noordam, Luba M Pardo, Diana Van Heemst, David A Gunn, Tamar Nijsten
BackgroundThe relationship between 25-hydroxyvitamin D and different phenotypes of skin aging (e.g., wrinkles, pigmented spots, telangiectasia, perceived age) is unclear. We investigated the association between vitamin D levels and skin aging phenotypes in middle-aged participants from the Rotterdam Study (RS) and Leiden Longevity Study (LLS).MethodsStandardized facial photographs were taken of North-European participants from the RS (N=3,831; 58.2% female, median age 66.5) and the LLS (N=661; 50.5% female, median age 63...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329650/increased-risk-of-opportunistic-infections-among-patients-with-moderate-to-severe-psoriasis-a-population-based-cohort-study-in-the-united-kingdom
#16
Junko Takeshita, Daniel B Shin, Alexis Ogdie, Joel M Gelfand
Infection is the second leading cause of death among psoriasis patients on phototherapy or systemic medications. The types of infections associated with psoriasis remain poorly understood. The aim of our study was to determine the risk of opportunistic infection (OI) among patients with psoriasis. We conducted a cohort study of patients with (N=199,700) and without (N=954,315) psoriasis in The Health Improvement Network electronic medical record database. Patients receiving phototherapy or systemic therapy were considered to have moderate-to-severe psoriasis (N=12,442)...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329648/hormonal-and-reproductive-factors-and-incidence-of-basal-cell-carcinoma-in-a-population-based-cohort
#17
Catherine M Olsen, Nirmala Pandeya, Bridie S Thompson, Jean Claude Dusingize, Padmini Subramaniam, Christina M Nagle, Adele C Green, Rachel E Neale, Penny M Webb, David C Whitman
Estrogens have photosensitizing properties, but few prospective studies have examined the relationship between hormonal and reproductive factors and incidence of basal cell carcinoma (BCC) in women. Previous findings have been inconsistent, particularly in relation to parity and use of oral contraceptives (OCs) and menopausal hormone therapy (MHT). Given the heterogeneity in the literature, we sought to examine the association between hormonal and reproductive factors and BCC risk in a cohort of women (n=23,879) enrolled in the QSkin Study in 2011...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329637/dermatological-screening-of-an-elderly-population-the-rotterdam-study
#18
Martijn G H Sanders, Joris A C Verkouteren, Luba M Pardo
BackgroundThe recent WHO burden of disease project showed that the non-fatal skin disease related burden is high, but also that prevalence rates of many common skin diseases are not well documented. The aim of this study is to give an overview of (untreated) skin diseases in a population based sample of elderly.MethodsIn 2010, a full body skin examination (FBSE) was embedded in the Rotterdam Study, a prospective population based cohort study of an elderly white-skinned population in the Netherlands. The examination was conducted by a dermatology-trained physician and focused on most common skin diseases (e...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329630/body-mass-index-height-and-early-onset-basal-cell-carcinoma-in-a-case-control-study
#19
Yanchang Zhang, Brenda Cartmel, Courtney C Choy, Annette M Molinaro, David J Leffell, Allen E Bale, Susan T Mayne, Leah M Ferucci
BackgroundBasal cell carcinoma (BCC) is the most common malignancy in the US. Body mass index (BMI) and height have been associated with a variety of cancer types, yet the evidence regarding BCC is limited. Therefore, we evaluated BMI and height in relation to early- onset BCC and explored the potential role of ultraviolet (UV) radiation exposure as well as estrogen-related exposures in the BMI-BCC relationship.MethodsBCC cases (n=377) diagnosed under age 40 were identified through a central dermatopathology facility in Connecticut...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329628/attitudes-and-predictors-of-sunless-tanning-in-a-sample-of-young-adults
#20
Chelsea Anderson, Brenda Cartmel, Susan T Mayne, Leah M Ferrucci
Given the popularity of a tan appearance, sunless tanning may play a role in skin cancer prevention as a substitute for ultraviolet (UV) tanning. Few studies have comprehensively assessed attitudes toward sunless tanning or predictors of use.Demographic, phenotypic, and lifestyle characteristics were ascertained during in-person interviews of 385 non-Hispanic whites (<age 40) who were controls in a case-control study of early-onset basal cell carcinoma. Attitudes toward sunless tanning were queried via self- administered questionnaire...
September 15, 2016: Dermatology Online Journal
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