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Xènia Crespo-Yàñez, Carmen Aguilar-Gurrieri, Anne-Claire Jacomin, Agnès Journet, Magda Mortier, Emmanuel Taillebourg, Emmanuelle Soleilhac, Winfried Weissenhorn, Marie-Odile Fauvarque
Integration and down-regulation of cell growth and differentiation signals rely on plasma membrane receptors endocytosis and sorting towards either recycling vesicles or degradative lysosomes via multivesicular bodies (MVB). In this process, the endosomal sorting complex-III required for transport (ESCRT-III) controls membrane deformation and scission triggering intraluminal vesicle (ILV) formation at early endosomes. Here, we show that the ESCRT-III member CHMP1B can be ubiquitinated within a flexible loop known to undergo conformational changes during polymerization...
June 22, 2018: PLoS Genetics
Teruhiko Imamura, Ann Nguyen, Ben Chung, Daniel Rodgers, Nitasha Sarswat, Gene Kim, Jayant Raikhelkar, Sirtaz Adatya, Takeyoshi Ota, Tae Song, Colleen Juricek, Jerry D Estep, Daniel Burkhoff, Valluvan Jeevanandam, Gabriel Sayer, Nir Uriel
The relationship between the HeartMate II left ventricular assist device (LVAD) position and pump thrombosis has been reported. However, further clinical implications of device position are unknown. This study aimed to investigate optimal device position for better left ventricular (LV) unloading and patient prognosis. Patients undergoing a ramp test with right heart catheterization after HeartMate II LVAD implantation were enrolled to this study. Device position was quantified from the chest X-ray obtained at the time of the ramp test: (1) inflow cannula angle relative to horizontal line, (2) pump angle relative to spine, (3) pump depth, (4) angle between inflow cannula and pump, and (5) angle between pump and outflow graft...
June 19, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Anke Schröder, Anastasia Benski, Anne Oltmanns, Ingo Just, Astrid Rohrbeck, Andreas Pich
Mono ADP-ribosylation is a common characteristic of bacterial toxins resulting to aberrant activation or inactivation of target proteins. The C3 exoenzyme of Clostridium botulinum (C3bot) ADP-ribosylates the small GTPases RhoA, RhoB and RhoC, leading to inactivation of these important regulators and impaired down-stream signaling. Quantification of ADP-ribosylation using gel migration assays, antibodies, and radioactivity-based methods are limited. Therefore a novel LC-MS-based method to specifically determine and quantify ADP-ribosylation of Rho GTPases was established...
June 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
David A Jacobsohn, Michael R Loken, Mingwei Fei, Alexia Adams, Lisa Eidenschink Brodersen, Brent R Logan, Kwang Woo Ahn, Bronwen E Shaw, Morris Kletzel, Marie Olszewski, Sana Khan, Soheil Meshinchi, Amy Keating, Andrew Harris, Pierre Teira, Reggie E Duerst, Steven P Margossian, Paul L Martin, Aleksandra Petrovic, Christopher C Dvorak, Eneida R Nemecek, Michael W Boyer, Allen R Chen, Jeffrey H Davis, Shalini Shenoy, Sureyya Savasan, Michelle P Hudspeth, Roberta H Adams, Victor A Lewis, Albert Kheradpour, Kimberly A Kasow, Alfred P Gillio, Ann E Haight, Monica Bhatia, Barbara J Bambach, Hilary L Haines, Troy C Quigg, Robert J Greiner, Julie-An Talano, David C Delgado, Alexandra Cheerva, Madhu Gowda, Sanjay Ahuja, Mehmet Ozkaynak, David Mitchell, Kirk R Schultz, Terry J Fry, David M Loeb, Michael A Pulsipher
We enrolled 150 patients in a prospective multi-center study of children with acute myeloid leukemia undergoing hematopoietic stem cell transplant (HSCT) comparing detection of measurable residual (MRD) disease by a "Difference from Normal" flow cytometry (ΔN) approach with assessment of Wilms tumor 1 (WT-1) gene expression without access to the diagnostic specimen. Prospective analysis of the specimens using this approach showed that 23% of patients being screened for HSCT had detectable residual disease by ΔN (0...
June 19, 2018: Biology of Blood and Marrow Transplantation
Valentina Di Liberto, R Maarten van Dijk, Matthias Brendel, Ann-Marie Waldron, Christina Möller, Ines Koska, Isabel Seiffert, Fabio Gualtieri, Franz Josef Gildehaus, Barbara von Ungern-Sternberg, Magdalena Lindner, Sibylle Ziegler, Rupert Palme, Rainer Hellweg, Peter Gass, Peter Bartenstein, Heidrun Potschka
Psychiatric comorbidities are prevalent in patients with epilepsy and greatly contribute to the overall burden of disease. The availability of reliable biomarkers to diagnose epilepsy-associated comorbidities would allow for effective treatment and improved disease management. Due to their non-invasive nature, molecular imaging techniques such as positron emission tomography (PET) are ideal tools to measure pathologic changes. In the current study we investigated the potential of [18 F]fluoro-2-deoxy-d-glucose ([18 F]FDG) and 2'-methoxyphenyl-(N-2'-pyridinyl)-p-18 F-fluoro-benzamidoethylpiperazine ([18 F]MPPF) as imaging correlates of neurobehavioral comorbidities in the pilocarpine rat model of epilepsy...
June 19, 2018: Neurobiology of Disease
Anne Brelot, Lisa A Chakrabarti
The chemokine receptor CCR5 has been the focus of intensive studies since its role as a coreceptor for HIV entry was discovered in 1996. These studies lead to the development of small molecular drugs targeting CCR5, with maraviroc becoming in 2007 the first clinically approved chemokine receptor inhibitor. More recently, the apparent HIV cure in a patient transplanted with hematopoietic stem cells devoid of functional CCR5 rekindled the interest for inactivating CCR5 through gene therapy and pharmacological approaches...
June 19, 2018: Journal of Molecular Biology
Veerle Foulon, Joke Wuyts, Franciska Desplenter, Anne Spinewine, Valérie Lacour, Dominique Paulus, Jan De Lepeleire
Background and objectives Patients often experience drug-related problems at admission or after discharge from hospital. The objective of this study was to identify the main problems in medication management at transition between settings of care, as experienced by health care professionals (HCPs) and patients. Methods Focus group discussions were organised between December 2009 and February 2010; nine focus groups with primary and secondary care HCPs and patients and two with stakeholders. Focus group discussions were audiotaped and observation files were constructed...
June 22, 2018: Acta Clinica Belgica
Meredith E Bohannon, Tom E Porter, Emma T Lavoie, Mary Ann Ottinger
The upper Hudson River was contaminated with polychlorinated biphenyls (PCB) Aroclor mixtures from the 1940s until the late 1970s. Several well-established biomarkers, such as induction of hepatic cytochrome P450 monooxygenases, were used to measure exposure to PCBs and similar contaminants in birds. In the present study, Japanese quail eggs were injected with a PCB mixture based upon a congener profile found in spotted sandpiper eggs at the upper Hudson River and subsequently, RNA was extracted from hatchling liver tissue for hybridization to a customized chicken cDNA microarray...
June 22, 2018: Journal of Toxicology and Environmental Health. Part A
Jacopo Troisi, Steven Symes, David Adair, Angelo Colucci, Sonia Elisa Prisco, Carmen Imma Aquino, Immacolata Vivone, Maurizio Guida, Sean Richards
Analysis of the human placenta metabolome has great potential to advance the understanding of complicated pregnancies and deleterious fetal outcomes in remote populations, but samples preparation can present unique challenges. Herein, we introduce oven-drying as a simple and widely available method of sample preparation that will facilitate investigations of the placental metabolome from remote and under-studied populations. Placentae from complicated and uncomplicated pregnancies were prepared in three ways (oven-dried at 60 °C, fresh, lyophilized) for metabolome analysis via gas chromatography-mass spectrometry (GC-MS)...
June 22, 2018: Preparative Biochemistry & Biotechnology
Neha Verma, Ann Marie Hurlburt, Angela Wolfe, Mun Kyoung Kim, Yoon Soon Kang, Jin Joo Kang, William E Stumph
In metazoans, U6 small nuclear RNA (snRNA) gene promoters utilize a proximal sequence element (PSE) recognized by the small nuclear RNA activating protein complex (SNAPc). SNAPc interacts with the transcription factor TFIIIB, which consists of the subunits TBP, Brf1 (Brf2 in vertebrates), and Bdp1. Here, we show that in Drosophila melanogaster, DmSNAPc directly recruits Bdp1 to the U6 promoter, and we identify an 87-residue region of Bdp1 involved in this interaction. Importantly, Bdp1 recruitment requires that DmSNAPc be bound to a U6 PSE rather than a U1 PSE...
June 22, 2018: FEBS Letters
Ulrica Nilsson, Elisabeth Ericsson, Mats Eriksson, Ewa Idvall, Ann-Cathrine Bramhagen
The study comprised a prospective, comparative cross-sectional survey in 143 (of 390) children undergoing tonsil surgery. Parents answered the Post Hospitalization Behavior Questionnaire for Ambulatory Surgery (PHBQ-AS), and children answered the questionnaire Postoperative Recovery in Children (PRiC). The PHBQ-AS had positive correlation with the PRiC and with general health. On day 10 after surgery, up to one-third of the children still reported physical symptoms (PRiC). No gender or age differences concerning the items of behavior (PHBQ-AS) were found...
January 1, 2018: Journal of Perioperative Practice
Valéry Ridde, Lara Gautier, Anne-Marie Turcotte-Tremblay, Isidore Sieleunou, Elisabeth Paul
Over the past 15 years, hundreds of millions of dollars have been invested in reforms founded on performance-based financing (PBF) in low- and middle-income countries. While evidence on its effectiveness and efficiency is still controversial, there appears to be an emerging consensus that equity has not been adequately considered. In this article, we show how PBF-type interventions in Africa have not sufficiently taken into account equity of access to care for the worst-off and their financial protection. In reviewing the history of health reforms in Africa, we show that this omission is nothing new...
January 1, 2018: International Journal of Health Services: Planning, Administration, Evaluation
Anne-Catherine Pouleur, Stefan Anker, Dulce Brito, Oana Brosteanu, Dirk Hasenclever, Barbara Casadei, Frank Edelmann, Gerasimos Filippatos, Damien Gruson, Ignatios Ikonomidis, Renaud Lhommel, Masliza Mahmod, Stefan Neubauer, Alexandre Persu, Bernhard L Gerber, Stefan Piechnik, Burkert Pieske, Elisabeth Pieske-Kraigher, Fausto Pinto, Piotr Ponikowski, Michele Senni, Jean-Noël Trochu, Nancy Van Overstraeten, Rolf Wachter, Jean-Luc Balligand
AIMS: Progressive left ventricular (LV) remodelling with cardiac myocyte hypertrophy, myocardial fibrosis, and endothelial dysfunction plays a key role in the onset and progression of heart failure with preserved ejection fraction. The Beta3-LVH trial will test the hypothesis that the β3 adrenergic receptor agonist mirabegron will improve LV hypertrophy and diastolic function in patients with hypertensive structural heart disease at high risk for developing heart failure with preserved ejection fraction...
June 22, 2018: ESC Heart Failure
Karen Head, Kornkiat Snidvongs, Simon Glew, Glenis Scadding, Anne Gm Schilder, Carl Philpott, Claire Hopkins
BACKGROUND: Allergic rhinitis is a common condition affecting both adults and children. Patients experience symptoms of nasal obstruction, rhinorrhoea, sneezing and nasal itching, which may affect their quality of life.Nasal irrigation with saline (salty water), also known as nasal douching, washing or lavage, is a procedure that rinses the nasal cavity with isotonic or hypertonic saline solutions. It can be performed with low positive pressure from a spray, pump or squirt bottle, with a nebuliser or with gravity-based pressure in which the person instils saline into one nostril and allows it to drain out of the other...
June 22, 2018: Cochrane Database of Systematic Reviews
Janet E Fulton, David M Buchner, Susan A Carlson, Deborah Borbely, Kenneth M Rose, Ann E O'Connor, Janelle P Gunn, Ruth Petersen
Physical activity can reduce the risk of at least 20 chronic diseases and conditions and provide effective treatment for many of these conditions. Yet, physical activity levels of Americans remain low, with only small improvements over 20 years. The Centers for Disease Control and Prevention (CDC) considered what would accelerate progress and, as a result, developed Active People, Healthy NationSM , an aspirational initiative to improve physical activity in 2.5 million high school youth and 25 million adults, doubling the 10-year improvement targets of Healthy People 2020...
June 22, 2018: Journal of Physical Activity & Health
Sebastian Wilde, Dongxin Ma, Tobias Koch, Anne Bakker, Dario Gonzalez-Abradelo, Linda Stegemann, Constantin G Daniliuc, Harald Fuchs, Hongying Gao, Nikos L Doltsinis, Lian Duan, Cristian A Strassert
The synthesis of new Pt(II) complexes bearing tailored cyclometalated C^N*N^C luminophores is reported along with their photophysical properties. The emission of the monomeric species can be blue shifted upon formal isosteric replacement of two C-H units by N atoms at the two cyclometalating rings. Their remarkable stability upon sublimation was demonstrated by means of scanning tunneling microscopy, which also revealed a defined self-assembly behavior leading to supramolecular arrays, showing a 3-fold symmetry in 2D-confined monolayers...
June 22, 2018: ACS Applied Materials & Interfaces
Hazal Boral, Anne van Diepeningen, Elif Erdem, Meltem Yağmur, G Sybren de Hoog, Macit Ilkit, Jacques F Meis, Abdullah M S Al-Hatmi
Owing to a lack of appropriate diagnostic and therapeutic approaches for mycotic keratitis, approximately one million cases of preventable corneal blindness are reported each year. The number of keratitis cases due to infection with Fusarium is increasing significantly worldwide, many of which are not treated adequately and in a timely manner due to frequent misdiagnosis. In the current report, we describe three cases of keratitis caused by Fusarium solani sensu stricto (FSSC5) from Turkey and The Netherlands, following ocular trauma...
June 21, 2018: Mycopathologia
Linda Björkhem-Bergman, Catharina Missailidis, John Karlsson-Valik, Ann Tammelin, Lena Ekström, Matteo Bottai, Ulf Hammar, Gudrun Lindh, Peter Bergman
Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to all beta-lactam antibiotics and can cause severe infections that are difficult to treat. Eradication strategies with conventional antibiotics are not always effective and alternative approaches are warranted. Here, we tested the hypothesis that daily supplementation with vitamin D for 12 months would reduce MRSA carriage rates among a group of persistent carriers. This was a double-blind, placebo-controlled randomized trial with n = 65 persistent MRSA carriers with 25-hydroxy vitamin D3 (25OHD) < 75 nmol/L, who were followed up with bacterial cultures at baseline and every 3 months for 1 year...
June 21, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Tingfeng Wu, Boqiang Qin, Guangwei Zhu, Timo Huttula, Antti Lindfors, Anne-Mari Ventelä, Yongwei Sheng, Richard F Ambrose
To address the contribution of long-term wind wave changes on diminishing ice period in Northern European lakes, an in situ observation of wind waves was conducted to calibrate a wind-wave numerical model for Lake Pyhäjärvi, which is the largest lake in southwest Finland. Using station-measured hydrometeorological data from 1963 to 2013 and model-simulated wind waves, correlation and regression analyses were conducted to assess the changing trend and main influences on ice period. Ice period in Lake Pyhäjärvi decreased significantly over 51 years (r = 0...
June 21, 2018: Environmental Science and Pollution Research International
Mette L K Schmidt, Peter Østergren, Prue Cormie, Anne-Mette Ragle, Jens Sønksen, Julie Midtgaard
PURPOSE: Regular exercise is recommended to mitigate the adverse effects of androgen deprivation therapy in men with prostate cancer. The purpose of this study was to explore the experience of transition to unsupervised, community-based exercise among men who had participated in a hospital-based supervised exercise programme in order to propose components that supported transition to unsupervised exercise. METHODS: Participants were selected by means of purposive, criteria-based sampling...
June 21, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
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