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https://www.readbyqxmd.com/read/28728298/-the-prognostic-analysis-of-hepatocellular-carcinoma-based-on-the-tumor-neo-vessels-macrophages-and-%C3%AE-sma-in-tumor-microenvironment
#1
M Fang, J P Yuan, L L Liu, G P Cheng, H J Ying, Y M Chen, M Chen
Objective: To analyze the quantitative expression and prognostic significance of tumor neo-vessels, macrophages and fibroblasts in tumor microenvironment of hepatocellular carcinoma (HCC). Methods: The clinic-pathological features and tissue samples for 101 HCC cases were collected. Immunohistochemistry was used to stain the tumor neo-vessels, macrophages and fibroblasts on tumor tissue. The distribution results and quantitative data of above key components were acquired by inverted microscopy equipped with CRi Nuance multispectral analysis system...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28726686/dual-mode-operation-highly-selective-nanohole-array-based-plasmonic-colour-filters
#2
Fatemeh Fouladi Mahani, Arash Mokhtari, Mahdiyeh Mehran
Taking advantage of nanostructured metal films as plasmonic colour filters has been evolved remarkably as an alternative to the conventional technologies of chemical colour filtering. However, most of the proposed plasmonic colour filters depict a poor colour purity focusing on the generating of either the additive or subtractive colours. In this paper, we present dual mode operation plasmonic colour filters employing an opaque aluminium film patterned with sub-wavelength holes. Subtractive colours like cyan, magenta, and yellow are the results of reflection mode of these filters yielding optical efficiencies as high as 70-80 percent and full width at half maximum of the stop-bands up to 40-50 nm...
July 20, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28724788/multiparametric-immune-profiling-in-hpv-oral-squamous-cell-cancer
#3
Zipei Feng, Daniel Bethmann, Matthias Kappler, Carmen Ballesteros-Merino, Alexander Eckert, R Bryan Bell, Allen Cheng, Tuan Bui, Rom Leidner, Walter J Urba, Kent Johnson, Clifford Hoyt, Carlo B Bifulco, Juergen Bukur, Claudia Wickenhauser, Barbara Seliger, Bernard A Fox
Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect of simultaneously evaluating T cell frequency and assessing suppressive elements and defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization of CD3+, CD8+, FoxP3+, CD163+, and PD-L1+ cells using multispectral imaging was performed on sections from 119 patients with HPV- OSCC. Expression of β2-microglobulin, MHC class I heavy chain, and large multifunctional peptidase 10 was quantified, and all data were correlated with patient outcome...
July 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28721307/multispectral-fluorescence-guided-surgery-a-feasibility-study-in-a-phantom-using-a-clinical-grade-laparoscopic-camera-system
#4
Danny M van Willigen, Nynke S van den Berg, Tessa Buckle, Gijs H KleinJan, James C Hardwick, Henk G van der Poel, Fijs Wb van Leeuwen
Although the possibilities in image guided surgery are advancing rapidly, complex surgical procedures such as nerve sparing prostatectomy still lack precision regarding differentiation between diseased and delicate anatomical structures. Here, the use of complementary fluorescent tracers in combination with a dedicated multispectral fluorescence camera system could support differentiation between healthy and diseased tissue. In this study, we provide proof of concept data indicating how a modified clinical-grade fluorescence laparoscope can be used to sensitively detect white light and three fluorescent dyes (fluorescein, Cy5, and ICG) in a sequential fashion...
2017: American Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28717575/flow-mediated-dilatation-test-using-optoacoustic-imaging-a-proof-of-concept
#5
Angelos Karlas, Josefine Reber, Gael Diot, Dmitry Bozhko, Maria Anastasopoulou, Tareq Ibrahim, Markus Schwaiger, Fabien Hyafil, Vasilis Ntziachristos
Label-free multispectral optoacoustic tomography (MSOT) has recently shown superior performance in visualizing the morphology of human vasculature, especially of smaller vessels, compared to ultrasonography. Herein, we extend these observations towards MSOT interrogation of macrovascular endothelial function. We employed a real-time handheld MSOT scanner to assess flow-mediated dilatation (FMD), a technique used to characterize endothelial function. A data processing scheme was developed to quantify the dimensions and diameter changes of arteries in humans and determine wall distensibility parameters...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28714584/oscillations-during-observations-dynamic-oscillatory-networks-serving-visuospatial-attention
#6
Alex I Wiesman, Elizabeth Heinrichs-Graham, Amy L Proskovec, Timothy J McDermott, Tony W Wilson
The dynamic allocation of neural resources to discrete features within a visual scene enables us to react quickly and accurately to salient environmental circumstances. A network of bilateral cortical regions is known to subserve such visuospatial attention functions; however the oscillatory and functional connectivity dynamics of information coding within this network are not fully understood. Particularly, the coding of information within prototypical attention-network hubs and the subsecond functional connections formed between these hubs have not been adequately characterized...
July 17, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28700137/dual-led-based-multichannel-microscopy-for-whole-slide-multiplane-multispectral-and-phase-imaging
#7
Jun Liao, Zhe Wang, Zibang Zhang, Zichao Bian, Kaikai Guo, Aparna Nambiar, Yutong Jiang, Shaowei Jiang, Jingang Zhong, Michael Choma, Guoan Zheng
We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data acquisition, achieving a maximum data throughput of ~1 gigapixel per second. To perform single-frame rapid autofocusing, we place two near-infrared LEDs at the back focal plane of the condenser lens to illuminate the sample from two different incident angles. A hot mirror is used to direct the near-infrared light to an autofocusing camera...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28688069/shoreline-change-assessment-using-multi-temporal-satellite-images-a-case-study-of-lake-sapanca-nw-turkey
#8
Umit Duru
The research summarized here determines historical shoreline changes along Lake Sapanca by using Remote Sensing (RS) and Geographical Information Systems (GIS). Six multi-temporal satellite images of Landsat Multispectral Scanner (L1-5 MMS), Enhanced Thematic Mapper Plus (L7 ETM+), and Operational Land Imager Sensors (L8 OLI), covering the period between 17 June 1975 and 15 July 2016, were used to monitor shoreline positions and estimate change rates along the coastal zone. After pre-possessing routines, the Normalized Difference Water Index (NDWI), Modified Normalized Difference Water Index (MNDWI), and supervised classification techniques were utilized to extract six different shorelines...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28686800/accelerated-three-dimensional-multispectral-mri-with-robust-principal-component-analysis-for-separation-of-on-and-off-resonance-signals
#9
Evan Levine, Kathryn Stevens, Christopher Beaulieu, Brian Hargreaves
PURPOSE: To enable highly accelerated distortion-free MRI near metal by separating on- and off-resonance to exploit the redundancy of slice-phase encoding for the dominant on-resonance component. METHODS: Multispectral MRI techniques resolve off-resonance distortions by a combination of limited excitation bins and additional encoding. Inspired by robust principal component analysis, a novel compact representation of multispectral images as a sum of rank-one and sparse matrices corresponding to on- and off-resonance respectively is described...
July 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28669429/hybrid-photoacoustic-ultrasound-tomograph-for-real-time-finger-imaging
#10
Milan Oeri, Wolfgang Bost, Nicolas Sénégond, Steffen Tretbar, Marc Fournelle
We report a target-enclosing, hybrid tomograph with a total of 768 elements based on capacitive micromachined ultrasound transducer technology and providing fast, high-resolution 2-D/3-D photoacoustic and ultrasound tomography tailored to finger imaging. A freely programmable ultrasound beamforming platform sampling data at 80 MHz was developed to realize plane wave transmission under multiple angles. A multiplexing unit enables the connection and control of a large number of elements. Fast image reconstruction is provided by GPU processing...
June 29, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28667994/vis-nir-multispectral-synchronous-imaging-pyrometer-for-high-temperature-measurements
#11
Tairan Fu, Jiangfan Liu, Jibin Tian
A visible-infrared multispectral synchronous imaging pyrometer was developed for simultaneous, multispectral, two-dimensional high temperature measurements. The multispectral image pyrometer uses prism separation construction in the spectrum range of 650-950 nm and multi-sensor fusion of three CCD sensors for high-temperature measurements. The pyrometer had 650-750 nm, 750-850 nm, and 850-950 nm channels all with the same optical path. The wavelength choice for each channel is flexible with three center wavelengths (700 nm, 810 nm, and 920 nm) with a full width at half maximum of the spectrum of 3 nm used here...
June 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28663918/optical-detection-and-monitoring-of-pigmented-skin-lesions
#12
J J Stamnes, G Ryzhikov, M Biryulina, B Hamre, L Zhao, K Stamnes
A method is presented for discriminating between malignant and benign pigmented skin lesions based on multispectral and multi-angle images. It is discussed how to retrieve maps of physiology properties and morphometric parameters from recorded images using a bio-optical model, radiative transfer calculations, and nonlinear inversion, and how to employ automated zooming to extract lesion and surrounding masks. Training and validation of a classification scheme for separation between benign and malignant tissue yielded sensitivity/specificity ranging from 97%/97% for application to a small dataset comprised of lesions not used for training and validation to 99%/93% for application to a larger dataset...
June 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28663914/multispectral-oximetry-of-murine-tendon-microvasculature-with-inflammation
#13
Marieke A van der Putten, James M Brewer, Andrew R Harvey
We report a novel multispectral imaging technique for localised measurement of vascular oxygen saturation (SO2) in vivo. Annular back-illumination is generated using a Schwarzchild-design reflective objective. Analysis of multispectral data is performed using a calibration-free oximetry algorithm. This technique is applied to oximetry in mice to measure SO2 in microvasculature supplying inflamed tendon tissue in the hind leg. Average SO2 for controls was 94.8 ± 7.0 % (N = 6), and 84.0 ± 13.5 % for mice with inflamed tendon tissue (N = 6)...
June 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28663864/measurement-of-multispectral-scattering-properties-in-mouse-brain-tissue
#14
Eunjung Min, Sungbea Ban, Yanyan Wang, Sung Chul Bae, Gabriel Popescu, Catherine Best-Popescu, Woonggyu Jung
We present the scattering properties of mouse brain using multispectral diffraction phase microscopy. Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm. The regional analysis was performed for both the myeloarchitecture and cytoarchitecture of the brain tissue. Our results clearly evaluate the multispectral scattering properties in the olfactory bulb and corpus callosum...
March 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28662242/multispectral-imaging-system-based-on-light-emitting-diodes-for-the-detection-of-melanomas-and-basal-cell-carcinomas-a-pilot-study
#15
Xana Delpueyo, Meritxell Vilaseca, Santiago Royo, Miguel Ares, Laura Rey-Barroso, Ferran Sanabria, Susana Puig, Giovanni Pellacani, Fernando Noguero, Giuseppe Solomita, Thierry Bosch
No abstract text is available yet for this article.
June 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28652976/noninvasive-real-time-characterization-of-non-melanoma-skin-cancers-with-handheld-optoacoustic-probes
#16
Amalina Binte Ebrahim Attia, Sai Yee Chuah, Daniel Razansky, Chris Jun Hui Ho, Pinky Malempati, U S Dinish, Renzhe Bi, Chit Yaw Fu, Steven J Ford, Joyce Siong-See Lee, Melissa Wee Ping Tan, Malini Olivo, Steven Tien Guan Thng
Currently, imaging technologies that enable dermsurgeons to visualize non-melanoma skin cancers (NMSC) in vivo preoperatively are lacking, resulting in excessive or incomplete removal. Multispectral optoacoustic tomography (MSOT) is a volumetric imaging tool to differentiate tissue chromophores and exogenous contrast agents, based on differences in their spectral signatures and used for high-resolution imaging of functional and molecular contrast at centimeter scale depth. We performed MSOT imaging with two- and three-dimensional handheld scanners on 21 Asian patients with NMSC...
September 2017: Photoacoustics
https://www.readbyqxmd.com/read/28641255/piecewise-linear-approximation-of-vector-valued-images-and-curves-via-second-order-variational-model
#17
Massimo Zanetti, Lorenzo Bruzzone
Variational models are known to work well for addressing image restoration/regularization problems. However, most of the methods proposed in the literature are defined for scalar inputs and are used on multiband images (such as RGB or multispectral imagery) by the composition of a simple band-wise processing. This involves suboptimal results and may introduce artifacts. Only in a few cases, variational models are extended to the case of vector-valued inputs. However, the known implementations are restricted to the first-order models, while the second-order models are never considered...
September 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28626793/acidic-ph-targeted-chitosan-capped-mesoporous-silica-coated-gold-nanorods-facilitate-detection-of-pancreatic-tumors-via-multispectral-optoacoustic-tomography
#18
Matthew R Zeiderman, Desiree E Morgan, John D Christein, William E Grizzle, Kelly M McMasters, Lacey R McNally
We present a cancer nanomedicine based on acidic pH targeted gold nanorods designed for multispectral optoacoustic tomography (MSOT). We have designed gold nanorods coated with mesoporous silica and subsequently capped with chitosan (CMGs). We have conjugated pH-sensitive variant 7 pHLIP peptide to the CMGs (V7-CMG) to provide targeting specificity to the acidic tumor microenvironment. In vitro, treatment of S2VP10 and MiaPaca2 cells with V7-CMG containing gemcitabine resulted in significantly greater cytotoxicity with 97% and 96...
July 11, 2016: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28617497/flexible-hybrid-graphene-a-si-h-multispectral-photodetectors
#19
D S Schneider, A Bablich, M C Lemme
We report on the integration of large area CVD grown single- and bilayer graphene transparent conductive electrodes (TCEs) on amorphous silicon multispectral photodetectors. The broadband transmission of graphene results in 440% enhancement of the detectors' spectral response in the ultraviolet (UV) region at λ = 320 nm compared to reference devices with conventional aluminum doped zinc oxide (ZnO:Al) electrodes. The maximum responsivity of the multispectral photodetectors can be tuned in their wavelength from 320 nm to 510 nm by an external bias voltage, allowing single pixel detection of UV to visible light...
June 29, 2017: Nanoscale
https://www.readbyqxmd.com/read/28615944/the-interactions-of-single-wall-carbon-nanohorns-with-polar-epithelium
#20
Yujie Shi, Zujin Shi, Suxin Li, Yuan Zhang, Bing He, Dong Peng, Jie Tian, Ming Zhao, Xueqing Wang, Qiang Zhang
Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site...
2017: International Journal of Nanomedicine
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