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https://www.readbyqxmd.com/read/29777103/bacterial-encapsulins-as-orthogonal-compartments-for-mammalian-cell-engineering
#1
Felix Sigmund, Christoph Massner, Philipp Erdmann, Anja Stelzl, Hannes Rolbieski, Mitul Desai, Sarah Bricault, Tobias P Wörner, Joost Snijder, Arie Geerlof, Helmut Fuchs, Martin Hrabĕ de Angelis, Albert J R Heck, Alan Jasanoff, Vasilis Ntziachristos, Jürgen Plitzko, Gil G Westmeyer
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulation of the enzyme tyrosinase leads to the confinement of toxic melanin production for robust detection via multispectral optoacoustic tomography (MSOT)...
May 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29777008/multispectral-fluorescence-imaging-as-a-tool-to-separate-healthy-and-disease-related-lymphatic-anatomies-during-robot-assisted-laparoscopic-procedures
#2
Philippa Meershoek, Gijs H KleinJan, Matthias N van Oosterom, Esther M Wit, Danny M van Willigen, Kevin P Bauwens, Erik J van Gennep, Alexandre M Mottrie, Henk G van der Poel, Fijs van Leeuwen
To reduce the invasive nature of extended pelvic lymph node dissections (ePLND) in prostate cancer, we have developed a multicolor fluorescence guidance approach that enables the discrimination between prostate draining lymph nodes (LNs) and lower limb draining LNs. Methods: In five porcine models multispectral-fluorescence guidance was performed using da Vinci Si- and Xi-robot consoles. They received fluorescein into the lower limb(s) and indocyanine green (ICG)-nanocolloid into the prostate. Results: Fluorescein was detected in 29 LNs (average of 3...
May 18, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29763828/combinatorial-multispectral-thermodynamics-docking-and-site-directed-mutagenesis-reveal-the-cognitive-characteristics-of-honey-bee-chemosensory-protein-to-plant-semiochemical
#3
Jing Tan, Xinmi Song, Xiaobin Fu, Fan Wu, Fuliang Hu, Hongliang Li
In the chemoreceptive system of insects, there are always some soluble binding proteins, such as some antennal-specific chemosensory proteins (CSPs), which are abundantly distributed in the chemosensory sensillar lymph. The antennal-specific CSPs usually have strong capability to bind diverse semiochemicals, while the detailed interaction between CSPs and the semiochemicals remain unclear. Here, by means of the combinatorial multispectral, thermodynamics, docking and site-directed mutagenesis, we detailedly interpreted a binding interaction between a plant semiochemical β-ionone and antennal-specific CSP1 from the worker honey bee...
May 9, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29762519/an-effective-palmprint-recognition-approach-for-visible-and-multispectral-sensor-images
#4
Abdu Gumaei, Rachid Sammouda, Abdul Malik Al-Salman, Ahmed Alsanad
Among several palmprint feature extraction methods the HOG-based method is attractive and performs well against changes in illumination and shadowing of palmprint images. However, it still lacks the robustness to extract the palmprint features at different rotation angles. To solve this problem, this paper presents a hybrid feature extraction method, named HOG-SGF that combines the histogram of oriented gradients (HOG) with a steerable Gaussian filter (SGF) to develop an effective palmprint recognition approach...
May 15, 2018: Sensors
https://www.readbyqxmd.com/read/29761000/quantitative-multispectral-ex-vivo-optical-evaluation-of-human-ovarian-tissue-using-spatial-frequency-domain-imaging
#5
Sreyankar Nandy, Ian S Hagemann, Matthew A Powell, Cary Siegel, Quing Zhu
About 85-90% of all ovarian cancers are carcinomas; these manifest clinically as mass-forming epithelial proliferations involving the ovary. In this study, a visible light spatial frequency domain imaging (SFDI) system was used for multispectral ex vivo imaging and quantitative evaluation of freshly excised benign and malignant human ovarian tissues. A total of 14 ovaries from 11 patients undergoing oophorectomy were investigated. Using a logistic regression model with seven significant spectral and spatial features extracted from SFDI images, a sensitivity of 94...
May 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29760980/optimization-of-wavelength-selection-for-multispectral-image-acquisition-a-case-study-of-atrial-ablation-lesions
#6
Huda Asfour, Shuyue Guan, Narine Muselimyan, Luther Swift, Murray Loew, Narine Sarvazyan
In vivo autofluorescence hyperspectral imaging of moving objects can be challenging due to motion artifacts and to the limited amount of acquired photons. To address both limitations, we selectively reduced the number of spectral bands while maintaining accurate target identification. Several downsampling approaches were applied to data obtained from the atrial tissue of adult pigs with sites of radiofrequency ablation lesions. Standard image qualifiers such as the mean square error, the peak signal-to-noise ratio, the structural similarity index map, and an accuracy index of lesion component images were used to quantify the effects of spectral binning, an increased spectral distance between individual bands, as well as random combinations of spectral bands...
May 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29760971/quantitative-characterization-of-turbidity-by-radiative-transfer-based-reflectance-imaging
#7
Peng Tian, Cheng Chen, Jiahong Jin, Heng Hong, Jun Q Lu, Xin-Hua Hu
A new and noncontact approach of multispectral reflectance imaging has been developed to inversely determine the absorption coefficient of μ a , the scattering coefficient of μs and the anisotropy factor g of a turbid target from one measured reflectance image. The incident beam was profiled with a diffuse reflectance standard for deriving both measured and calculated reflectance images. A GPU implemented Monte Carlo code was developed to determine the parameters with a conjugate gradient descent algorithm and the existence of unique solutions was shown...
May 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29746473/detection-of-rice-sheath-blight-using-an-unmanned-aerial-system-with-high-resolution-color-and-multispectral-imaging
#8
Dongyan Zhang, Xingen Zhou, Jian Zhang, Yubin Lan, Chao Xu, Dong Liang
Detection and monitoring are the first essential step for effective management of sheath blight (ShB), a major disease in rice worldwide. Unmanned aerial systems have a high potential of being utilized to improve this detection process since they can reduce the time needed for scouting for the disease at a field scale, and are affordable and user-friendly in operation. In this study, a commercialized quadrotor unmanned aerial vehicle (UAV), equipped with digital and multispectral cameras, was used to capture imagery data of research plots with 67 rice cultivars and elite lines...
2018: PloS One
https://www.readbyqxmd.com/read/29745130/intraoperative-hyperspectral-determination-of-human-tissue-properties
#9
Eric Larry Wisotzky, Florian Cornelius Uecker, Philipp Arens, Steffen Dommerich, Anna Hilsmann, Peter Eisert
We address the automatic differentiation of human tissue using multispectral imaging with promising potential for automatic visualization during surgery. Currently, tissue types have to be continuously differentiated based on the surgeon's knowledge only. Further, automatic methods based on optical in vivo properties of human tissue do not yet exist, as these properties have not been sufficiently examined. To overcome this, we developed a hyperspectral camera setup to monitor the different optical behavior of tissue types in vivo...
May 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29743577/long-lived-monolithic-micro-optics-for-multispectral-grin-applications
#10
Antoine Lepicard, Flavie Bondu, Myungkoo Kang, Laura Sisken, Anupama Yadav, Frederic Adamietz, Vincent Rodriguez, Kathleen Richardson, Marc Dussauze
The potential for realizing robust, monolithic, near-surface refractive micro-optic elements with long-lived stability is demonstrated in visible and infrared transmitting glasses capable of use in dual band applications. Employing an enhanced understanding of glass chemistry and geometric control of mobile ion migration made possible with electrode patterning, flat, permanent, thermally-poled micro-optic structures have been produced and characterized. Sub-surface (t~5-10 µm) compositional and structural modification during the poling process results in formation of spatially-varying refractive index profiles, exhibiting induced Δn changes up to 5 × 10-2 which remain stable for >15 months...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29736563/multispectral-photoacoustic-imaging-of-tumor-protease-activity-with-a-gold-nanocage-based-activatable-probe
#11
Cheng Liu, Shiying Li, Yanjuan Gu, Huahua Xiong, Wing-Tak Wong, Lei Sun
PURPOSE: Tumor proteases have been recognized as significant regulators in the tumor microenvironment, but the current strategies for in vivo protease imaging have tended to focus on the development of a probe design rather than the investigation of a novel imaging strategy by leveraging the imaging technique and probe. Herein, it is the first report to investigate the ability of multispectral photoacoustic imaging (PAI) to estimate the distribution of protease cleavage sites inside living tumor tissue by using an activatable photoacoustic (PA) probe...
May 7, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29735948/filter-selection-for-optimizing-the-spectral-sensitivity-of-broadband-multispectral-cameras-based-on-maximum-linear-independence
#12
Sui-Xian Li
Previous research has shown that the effectiveness of selecting filter sets from among a large set of commercial broadband filters by a vector analysis method based on maximum linear independence (MLI). However, the traditional MLI approach is suboptimal due to the need to predefine the first filter of the selected filter set to be the maximum ℓ₂ norm among all available filters. An exhaustive imaging simulation with every single filter serving as the first filter is conducted to investigate the features of the most competent filter set...
May 7, 2018: Sensors
https://www.readbyqxmd.com/read/29734747/visible-and-extended-near-infrared-multispectral-imaging-for-skin-cancer-diagnosis
#13
Laura Rey-Barroso, Francisco J Burgos-Fernández, Xana Delpueyo, Miguel Ares, Santiago Royo, Josep Malvehy, Susana Puig, Meritxell Vilaseca
With the goal of diagnosing skin cancer in an early and noninvasive way, an extended near infrared multispectral imaging system based on an InGaAs sensor with sensitivity from 995 nm to 1613 nm was built to evaluate deeper skin layers thanks to the higher penetration of photons at these wavelengths. The outcomes of this device were combined with those of a previously developed multispectral system that works in the visible and near infrared range (414 nm⁻995 nm). Both provide spectral and spatial information from skin lesions...
May 5, 2018: Sensors
https://www.readbyqxmd.com/read/29734072/a-dilute-and-shoot-multispectral-integration-approach-towards-nontargeted-component-profiling-of-traditional-herbal-yin-zhi-huang-using-liquid-chromatography-photodiode-array-ion-trap-time-of-flight-characterization
#14
Meiling Wang, Yu Liu, Bin Guo, Lijuan Wei, Hao Teng, Xiaoqing Chen, Ming Ma
Ying-zhi-huang Injection (YZH-I) is a classic intravenous formulation of polyherbal Chinese medicine which has been prescribed to treat severe jaundice and acute hepatitis for nearly 50 years. Despite some published data on its major components in the constituent herbs, the overall chemical profile of the potentially bioactive ingredients in YZH-I formula remains largely unknown. Here we developed a multispectral integration approach towards nontargeted phytochemical profiling of YZH-I by liquid chromatography-ultraviolet diode array detector coupled to ion trap/time-of-flight multistage mass spectrometry (LC-DAD-IT(MSn )/TOF) with fast polarity-switching mode...
April 27, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29726513/measurement-of-two-dimensional-thickness-of-micro-patterned-thin-film-based-on-image-restoration-in-a-spectroscopic-imaging-reflectometer
#15
Min-Gab Kim, Jin-Yong Kim
In this paper, we introduce a method to overcome the limitation of thickness measurement of a micro-patterned thin film. A spectroscopic imaging reflectometer system that consists of an acousto-optic tunable filter, a charge-coupled-device camera, and a high-magnitude objective lens was proposed, and a stack of multispectral images was generated. To secure improved accuracy and lateral resolution in the reconstruction of a two-dimensional thin film thickness, prior to the analysis of spectral reflectance profiles from each pixel of multispectral images, the image restoration based on an iterative deconvolution algorithm was applied to compensate for image degradation caused by blurring...
May 1, 2018: Applied Optics
https://www.readbyqxmd.com/read/29726109/dual-modality-surface-enhanced-resonance-raman-scattering-and-multispectral-optoacoustic-tomography-nanoparticle-approach-for-brain-tumor-delineation
#16
Volker Neuschmelting, Stefan Harmsen, Nicolas Beziere, Hannah Lockau, Hsiao-Ting Hsu, Ruimin Huang, Daniel Razansky, Vasilis Ntziachristos, Moritz F Kircher
Difficulty in visualizing glioma margins intraoperatively remains a major issue in the achievement of gross total tumor resection and, thus, better clinical outcome of glioblastoma (GBM) patients. Here, the potential of a new combined optical + optoacoustic imaging method for intraoperative brain tumor delineation is investigated. A strategy using a newly developed gold nanostar synthesis method, Raman reporter chemistry, and silication method to produce dual-modality contrast agents for combined surface-enhanced resonance Raman scattering (SERRS) and multispectral optoacoustic tomography (MSOT) imaging is devised...
May 4, 2018: Small
https://www.readbyqxmd.com/read/29721735/evaluation-of-anthocyanins-in-aronia-melanocarpa-bsa-binding-by-spectroscopic-studies
#17
Jie Wei, Dexin Xu, Xiao Zhang, Jing Yang, Qiuyu Wang
The interaction between Anthocyanins in Aronia melanocarpa (AMA) and bovine serum albumin (BSA) were studied in this paper by multispectral technology, such as fluorescence quenching titration, circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FTIR). The results of the fluorescence titration revealed that AMA could strongly quench the intrinsic fluorescence of BSA by static quenching. The apparent binding constants KSV and number of binding sites n of AMA with BSA were obtained by fluorescence quenching method...
May 2, 2018: AMB Express
https://www.readbyqxmd.com/read/29716112/hybrid-metasurfaces-for-microwave-reflection-and-infrared-emission-reduction
#18
Yongqiang Pang, Yongfeng Li, Mingbao Yan, Dongqing Liu, Jiafu Wang, Zhuo Xu, Shaobo Qu
Controlling of electromagnetic wave radiation is of great importance in many fields. In this work, a hybrid metasurface (HMS) is designed to simultaneously reduce the microwave reflection and the infrared emission. The HMS is composed of the metal/dielectric/metal/dielectric/metal configuration. The reflection reduction at microwave frequencies mainly results from the phase cancellation technique, while the infrared emission reduction is due to the reflection of the metal with a high filling ration in the top layer...
April 30, 2018: Optics Express
https://www.readbyqxmd.com/read/29716046/designing-optical-3d-images-encryption-and-reconstruction-using-monospectral-synthetic-aperture-integral-imaging
#19
Xiaowei Li, Min Zhao, Yan Xing, Han-Le Zhang, Lei Li, Seok-Tae Kim, Xin Zhou, Qiong-Hua Wang
This paper realizes an optical 3D images encryption and reconstruction by employing the geometric calibration algorithm to the monospectral synthetic aperture integral imaging system. This method has the simultaneous advantages of improving the quality of 3D images by eliminating the crosstalk from the unaligned cameras and increasing security of the multispectral 3D images encryption by importing the random generated maximum-length cellular automata into the Fresnel transform encoding algorithm. Furthermore, compared with the previous 3D images encryption methods of encrypting 3D multispectral information, the proposed method only encrypts monospectral data, which will greatly minimize the complexity...
April 30, 2018: Optics Express
https://www.readbyqxmd.com/read/29715990/time-resolved-multispectral-imaging-based-on-an-adaptive-single-pixel-camera
#20
Florian Rousset, Nicolas Ducros, Françoise Peyrin, Gianluca Valentini, Cosimo D'Andrea, Andrea Farina
Time-resolved multispectral imaging has many applications in different fields, which range from characterization of biological tissues to environmental monitoring. In particular, optical techniques, such as lidar and fluorescence lifetime imaging, require imaging at the subnanosecond scales over an extended area. In this paper, we demonstrate experimentally a time-resolved multispectral acquisition scheme based on single-pixel imaging. Single-pixel imaging is an emerging paradigm that provides low-cost high-quality images...
April 16, 2018: Optics Express
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