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https://www.readbyqxmd.com/read/28815503/imaging-neural-architecture-in-brainbow-samples
#1
Douglas H Roossien, Dawen Cai
The fluorescent protein revolution has made the light microscope the most widely used tool for studying biological structure from the single-molecule to whole organism scales. However, traditional approaches are limited in their ability to resolve components in highly complex structures, such as the brain. In recent years, this limitation has been circumvented by the development of multicolor labeling methods, termed Brainbow. Brainbow tools rely on site-specific recombinases to make stochastic "choices" between different combinations of fluorescent proteins so that structures in close proximity to one another can be resolved based on their color profile...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809848/a-multimodal-dataset-for-various-forms-of-distracted-driving
#2
Salah Taamneh, Panagiotis Tsiamyrtzis, Malcolm Dcosta, Pradeep Buddharaju, Ashik Khatri, Michael Manser, Thomas Ferris, Robert Wunderlich, Ioannis Pavlidis
We describe a multimodal dataset acquired in a controlled experiment on a driving simulator. The set includes data for n=68 volunteers that drove the same highway under four different conditions: No distraction, cognitive distraction, emotional distraction, and sensorimotor distraction. The experiment closed with a special driving session, where all subjects experienced a startle stimulus in the form of unintended acceleration-half of them under a mixed distraction, and the other half in the absence of a distraction...
August 15, 2017: Scientific Data
https://www.readbyqxmd.com/read/28802047/dual-ifgmosaic-a-versatile-method-for-multispectral-and-combinatorial-mosaic-gene-function-analysis
#3
Samuel Pontes-Quero, Luis Heredia, Verónica Casquero-García, Macarena Fernández-Chacón, Wen Luo, Ana Hermoso, Mayank Bansal, Irene Garcia-Gonzalez, Maria S Sanchez-Muñoz, Juan R Perea, Adrian Galiana-Simal, Iker Rodriguez-Arabaolaza, Sergio Del Olmo-Cabrera, Susana F Rocha, Luis M Criado-Rodriguez, Giovanna Giovinazzo, Rui Benedito
Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue...
August 10, 2017: Cell
https://www.readbyqxmd.com/read/28800065/validating-modis-and-sentinel-2-ndvi-products-at-a-temperate-deciduous-forest-site-using-two-independent-ground-based-sensors
#4
Maximilian Lange, Benjamin Dechant, Corinna Rebmann, Michael Vohland, Matthias Cuntz, Daniel Doktor
Quantifying the accuracy of remote sensing products is a timely endeavor given the rapid increase in Earth observation missions. A validation site for Sentinel-2 products was hence established in central Germany. Automatic multispectral and hyperspectral sensor systems were installed in parallel with an existing eddy covariance flux tower, providing spectral information of the vegetation present at high temporal resolution. Normalized Difference Vegetation Index (NDVI) values from ground-based hyperspectral and multispectral sensors were compared with NDVI products derived from Sentinel-2A and Moderate-resolution Imaging Spectroradiometer (MODIS)...
August 11, 2017: Sensors
https://www.readbyqxmd.com/read/28783623/kronecker-basis-representation-based-tensor-sparsity-and-its-applications-to-tensor-recovery
#5
Qi Xie, Qian Zhao, Deyu Meng, Zongben Xu
It is well known that the sparsity/low-rank of a vector/matrix can be rationally measured by nonzero-entries-number ($l_0$ norm)/nonzero- singular-values-number (rank), respectively. However, data from real applications are often generated by the interaction of multiple factors, which obviously cannot be sufficiently represented by a vector/matrix, while a high order tensor is expected to provide more faithful representation to deliver the intrinsic structure underlying such data ensembles. Unlike the vector/matrix case, constructing a rational high order sparsity measure for tensor is a relatively harder task...
August 2, 2017: IEEE Transactions on Pattern Analysis and Machine Intelligence
https://www.readbyqxmd.com/read/28780276/uv-imaging-of-multiple-unit-pellet-system-mups-tablets-a-case-study-of-acetylsalicylic-acid-stability
#6
Anna Novikova, Jens M Carstensen, Thomas Rades, Claudia S Leopold
The applicability of multispectral ultraviolet (UV) imaging in combination with multivariate image analysis was investigated to monitor API degradation within multiple unit pellet system (MUPS) tablets during storage. For this purpose, acetylsalicylic acid (ASA) layered pellets were coated with Eudragit(®) RL PO and compressed to MUPS tablets. These tablets were stored under four different conditions with different levels of relative humidity (0 and 75%) and temperature (21 and 40 °C) and analysed at seven storage time points (0, 15, 40, 140, 165, 265, and 330 d)...
August 2, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28762952/prediction-of-radio-responsiveness-with-immune-profiling-in-patients-with-rectal-cancer
#7
In Ja Park, Soyeon An, Sang-Yeob Kim, Hye Min Lim, Seung-Mo Hong, Mi-Ju Kim, Yun Jae Kim, Chang Sik Yu
We evaluate whether the tumor immune infiltrate (TIL) could be used for prediction of responsiveness to preoperative chemoradiotherapy (PCRT) in rectal cancers. Using formalin-fixed paraffin-embedded slides of pretreatment biopsies, co-stain for CD4, CD8, CD274 (PD-L1), FOXP3, cytokeratin, and DAPI was performed with Opal multi staining kit (Perkin-Elmer, Waltham, MA). Multispectral imaging and digital analysis to visualize and quantify specific immune infiltrates were performed using the Vectra imaging system (Perkin-Elmer)...
July 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28758984/assessment-of-chlorophyll-a-algorithms-considering-different-trophic-statuses-and-optimal-bands
#8
Salem Ibrahim Salem, Hiroto Higa, Hyungjun Kim, Hiroshi Kobayashi, Kazuo Oki, Taikan Oki
Numerous algorithms have been proposed to retrieve chlorophyll-a concentrations in Case 2 waters; however, the retrieval accuracy is far from satisfactory. In this research, seven algorithms are assessed with different band combinations of multispectral and hyperspectral bands using linear (LN), quadratic polynomial (QP) and power (PW) regression approaches, resulting in altogether 43 algorithmic combinations. These algorithms are evaluated by using simulated and measured datasets to understand the strengths and limitations of these algorithms...
July 31, 2017: Sensors
https://www.readbyqxmd.com/read/28755860/dynamic-and-accurate-assessment-of-acetaminophen-induced-hepatotoxicity-by-integrated-photoacoustic-imaging-and-mechanistic-biomarkers-in-vivo
#9
Nathalie Brillant, Mohamed Elmasry, Neal C Burton, Josep Monne Rodriguez, Jack W Sharkey, Stephen Fenwick, Harish Poptani, Neil R Kitteringham, Christopher E Goldring, Anja Kipar, B Kevin Park, Daniel J Antoine
The prediction and understanding of acetaminophen (APAP)-induced liver injury (APAP-ILI) and the response to therapeutic interventions is complex. This is due in part to sensitivity and specificity limitations of currently used assessment techniques. Here we sought to determine the utility of integrating translational non-invasive photoacoustic imaging of liver function with mechanistic circulating biomarkers of hepatotoxicity with histological assessment to facilitate the more accurate and precise characterization of APAP-ILI and the efficacy of therapeutic intervention...
July 26, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28750131/multispectral-imaging-system-based-on-light-emitting-diodes-for-the-detection-of-melanomas-and-basal-cell-carcinomas-a-pilot-study-erratum
#10
Xana Delpueyo, Meritxell Vilaseca, Santiago Royo, Miguel Ares, Laura Rey-Barroso, Ferran Sanabria, Susana Puig, Josep Malvehy, Giovanni Pellacani, Fernando Noguero, Giuseppe Solomita, Thierry Bosch
No abstract text is available yet for this article.
July 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28738734/a-tissue-factor-cascade-targeted-nanoparticle-forsite-directed-inducing-thrombosis
#11
Weiwei Jin, Yanxue Yin, Bo Zhang, Heng Mei, Huafang Wang, Tao Guo, Wei Shi, Yu Hu
Tissue factor is an upstream component of the cascade and a high-expressing factor under phathological conditions. In this study, a tissue factor cascade-targeted strategy for inducing local thrombosis was developed by combining ENP-HMME and photochemistry. In vitro study showed that protein EGFP-EGF1 conjugation to the nanoparticles could significantly contribute to the uptake of nanoparticles by tissue factor over-expressed brain capillary endothelial cells. Three-dimensional imaging and specklegram of brains in vivo showed that tissue factor cascade-targeted strategy successfully induced thrombosis of expected position...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28730846/spectral-behavior-of-white-pigment-mixtures-using-reflectance-ultraviolet-fluorescence-spectroscopy-and-multispectral-imaging
#12
Lucilla Pronti, Anna Candida Felici, Matthieu Ménager, Cathy Vieillescazes, Mario Piacentini
Reflectance spectroscopy, ultraviolet (UV)-fluorescence spectroscopy, and multispectral imaging have been widely employed for pigment identification on paintings. From ancient times to the present, lead white, zinc white, and titanium white have been the most important white pigments used for paintings and they are used as pigment markers for dating a work of art. The spectral behavior of these pigments is reported in several scientific papers and websites, but those of their mixtures are quite unknown. We present a combined nondestructive approach for identifying mixtures of lead white, zinc white, and titanium white as powder and dispersed in two different binder media (egg yolk and linseed oil) by using reflectance spectroscopy, spectrofluorimetry, multispectral reflectance and UV-fluorescence imaging...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28728298/-the-prognostic-analysis-of-hepatocellular-carcinoma-based-on-the-tumor-neo-vessels-macrophages-and-%C3%AE-sma-in-tumor-microenvironment
#13
M Fang, J P Yuan, L L Liu, G P Cheng, H J Ying, Y M Chen, M Chen
Objective: To analyze the quantitative expression and prognostic significance of tumor neo-vessels, macrophages and fibroblasts in tumor microenvironment of hepatocellular carcinoma (HCC). Methods: The clinic-pathological features and tissue samples for 101 HCC cases were collected. Immunohistochemistry was used to stain the tumor neo-vessels, macrophages and fibroblasts on tumor tissue. The distribution results and quantitative data of above key components were acquired by inverted microscopy equipped with CRi Nuance multispectral analysis system...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28726686/dual-mode-operation-highly-selective-nanohole-array-based-plasmonic-colour-filters
#14
Fatemeh Fouladi Mahani, Arash Mokhtari, Mahdiyeh Mehran
Taking advantage of nanostructured metal films as plasmonic colour filters has been evolved remarkably as an alternative to the conventional technologies of chemical colour filtering. However, most of the proposed plasmonic colour filters depict a poor colour purity focusing on the generating of either the additive or subtractive colours. In this paper, we present dual mode operation plasmonic colour filters employing an opaque aluminium film patterned with sub-wavelength holes. Subtractive colours like cyan, magenta, and yellow are the results of reflection mode of these filters yielding optical efficiencies as high as 70-80 percent and full width at half maximum of the stop-bands up to 40-50 nm...
July 20, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28724788/multiparametric-immune-profiling-in-hpv-oral-squamous-cell-cancer
#15
Zipei Feng, Daniel Bethmann, Matthias Kappler, Carmen Ballesteros-Merino, Alexander Eckert, R Bryan Bell, Allen Cheng, Tuan Bui, Rom Leidner, Walter J Urba, Kent Johnson, Clifford Hoyt, Carlo B Bifulco, Juergen Bukur, Claudia Wickenhauser, Barbara Seliger, Bernard A Fox
Evaluation of T lymphocyte frequency provides prognostic information for patients with oral squamous cell cancer (OSCC). However, the effect of simultaneously evaluating T cell frequency and assessing suppressive elements and defects in antigen-processing machinery (APM) has not been clarified. Simultaneous characterization of CD3+, CD8+, FoxP3+, CD163+, and PD-L1+ cells using multispectral imaging was performed on sections from 119 patients with HPV- OSCC. Expression of β2-microglobulin, MHC class I heavy chain, and large multifunctional peptidase 10 was quantified, and all data were correlated with patient outcome...
July 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28721307/multispectral-fluorescence-guided-surgery-a-feasibility-study-in-a-phantom-using-a-clinical-grade-laparoscopic-camera-system
#16
Danny M van Willigen, Nynke S van den Berg, Tessa Buckle, Gijs H KleinJan, James C Hardwick, Henk G van der Poel, Fijs Wb van Leeuwen
Although the possibilities in image guided surgery are advancing rapidly, complex surgical procedures such as nerve sparing prostatectomy still lack precision regarding differentiation between diseased and delicate anatomical structures. Here, the use of complementary fluorescent tracers in combination with a dedicated multispectral fluorescence camera system could support differentiation between healthy and diseased tissue. In this study, we provide proof of concept data indicating how a modified clinical-grade fluorescence laparoscope can be used to sensitively detect white light and three fluorescent dyes (fluorescein, Cy5, and ICG) in a sequential fashion...
2017: American Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28717575/flow-mediated-dilatation-test-using-optoacoustic-imaging-a-proof-of-concept
#17
Angelos Karlas, Josefine Reber, Gael Diot, Dmitry Bozhko, Maria Anastasopoulou, Tareq Ibrahim, Markus Schwaiger, Fabien Hyafil, Vasilis Ntziachristos
Label-free multispectral optoacoustic tomography (MSOT) has recently shown superior performance in visualizing the morphology of human vasculature, especially of smaller vessels, compared to ultrasonography. Herein, we extend these observations towards MSOT interrogation of macrovascular endothelial function. We employed a real-time handheld MSOT scanner to assess flow-mediated dilatation (FMD), a technique used to characterize endothelial function. A data processing scheme was developed to quantify the dimensions and diameter changes of arteries in humans and determine wall distensibility parameters...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28714584/oscillations-during-observations-dynamic-oscillatory-networks-serving-visuospatial-attention
#18
Alex I Wiesman, Elizabeth Heinrichs-Graham, Amy L Proskovec, Timothy J McDermott, Tony W Wilson
The dynamic allocation of neural resources to discrete features within a visual scene enables us to react quickly and accurately to salient environmental circumstances. A network of bilateral cortical regions is known to subserve such visuospatial attention functions; however the oscillatory and functional connectivity dynamics of information coding within this network are not fully understood. Particularly, the coding of information within prototypical attention-network hubs and the subsecond functional connections formed between these hubs have not been adequately characterized...
July 17, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28700137/dual-light-emitting-diode-based-multichannel-microscopy-for-whole-slide-multiplane-multispectral-and-phase-imaging
#19
Jun Liao, Zhe Wang, Zibang Zhang, Zichao Bian, Kaikai Guo, Aparna Nambiar, Yutong Jiang, Shaowei Jiang, Jingang Zhong, Michael Choma, Guoan Zheng
We report the development of a multichannel microscopy for whole-slide multiplane, multispectral and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data acquisition, achieving a maximum data throughput of approximately 1 gigapixel per second. To perform single-frame rapid autofocusing, we place 2 near-infrared light-emitting diodes (LEDs) at the back focal plane of the condenser lens to illuminate the sample from 2 different incident angles...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28688069/shoreline-change-assessment-using-multi-temporal-satellite-images-a-case-study-of-lake-sapanca-nw-turkey
#20
Umit Duru
The research summarized here determines historical shoreline changes along Lake Sapanca by using Remote Sensing (RS) and Geographical Information Systems (GIS). Six multi-temporal satellite images of Landsat Multispectral Scanner (L1-5 MMS), Enhanced Thematic Mapper Plus (L7 ETM+), and Operational Land Imager Sensors (L8 OLI), covering the period between 17 June 1975 and 15 July 2016, were used to monitor shoreline positions and estimate change rates along the coastal zone. After pre-possessing routines, the Normalized Difference Water Index (NDWI), Modified Normalized Difference Water Index (MNDWI), and supervised classification techniques were utilized to extract six different shorelines...
August 2017: Environmental Monitoring and Assessment
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