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https://www.readbyqxmd.com/read/28913168/feasibility-of-photoacoustic-ultrasound-imaging-of-synovitis-in-finger-joints-using-a-point-of-care-system
#1
Pim J van den Berg, Khalid Daoudi, Hein J Bernelot Moens, Wiendelt Steenbergen
We evaluate a portable ultrasound and photoacoustic imaging (PAI) system for the feasibility of a point-of-care assessment of clinically evident synovitis. Inflamed and non-inflamed proximal interphalangeal joints of 10 patients were examined and compared with joints from 7 healthy volunteers. PAI scans, ultrasound power Doppler (US-PD), and clinical examination were performed. We quantified the amount of photoacoustic (PA) signal using a region of interest (ROI) drawn over the hypertrophic joint space. PAI response was increased 4 to 10 fold when comparing inflamed with contralateral non-inflamed joints and with joints from healthy volunteers (p < 0...
December 2017: Photoacoustics
https://www.readbyqxmd.com/read/28900264/measuring-spectral-inconsistency-of-multispectral-images-for-detection-and-segmentation-of-retinal-degenerative-changes
#2
Jian Lian, Yuanjie Zheng, Peiyong Duan, Wanzhen Jiao, Bojun Zhao, Yanju Ren, Dinggang Shen
Multispectral imaging (MSI) creates a series of en-face fundus spectral sections by leveraging an extensive range of discrete monochromatic light sources and allows for an examination of the retina's early morphologic changes that are not generally visible with traditional fundus imaging modalities. An Ophthalmologist's interpretation of MSI images is commonly conducted by qualitatively analyzing the spectral consistency between degenerated areas and normal ones, which characterizes the image variation across different spectra...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28886801/noninvasive-technologies-for-the-diagnosis-of-cutaneous-melanoma
#3
REVIEW
Richard R Winkelmann, Aaron S Farberg, Alex M Glazer, Darrell S Rigel
Multispectral analysis devices assess pigmented lesion disorganization at different levels using variable wavelengths of light. Computerized algorithms measure morphologic disorganization of the pigmented skin lesion. Aggregated data of 855 participants investigating the influence of multispectral digital skin lesion analysis (MSDSLA) on practitioner decisions to biopsy pigmented skin lesions revealed the overall sensitivity for detection of melanoma improved from 70% to 88%. Participant specificity increased from 52% to 58% after MSDSLA...
October 2017: Dermatologic Clinics
https://www.readbyqxmd.com/read/28883396/in-vivo-quantitative-imaging-of-tumor-ph-by-nanosonophore-assisted-multispectral-photoacoustic-imaging
#4
Janggun Jo, Chang H Lee, Raoul Kopelman, Xueding Wang
Changes of physiological pH are correlated with several pathologies, therefore the development of more effective medical pH imaging methods is of paramount importance. Here, we report on an in vivo pH mapping nanotechnology. This subsurface chemical imaging is based on tumor-targeted, pH sensing nanoprobes and multi-wavelength photoacoustic imaging (PAI). The nanotechnology consists of an optical pH indicator, SNARF-5F, 5-(and-6)-Carboxylic Acid, encapsulated into polyacrylamide nanoparticles with surface modification for tumor targeting...
September 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28876398/remote-sensing-of-the-chlorophyll-a-based-on-oli-landsat-8-and-msi-sentinel-2a-barra-bonita-reservoir-brazil
#5
Fernanda Watanabe, Enner Alcântara, Thanan Rodrigues, Luiz Rotta, Nariane Bernardo, Nilton Imai
In this present research, we assessed the performance of band algorithms in estimating chlorophyll-a (Chl-a) concentration based on bands of two new sensors: Operational Land Imager onboard Landsat-8 satellite (OLI/Landsat-8), and MultiSpectral Instrument onboard Sentinel-2A (MSI/Sentinel-2A). Band combinations designed for Thematic Mapper onboard Landsat-5 satellite (TM/Landsat-5) and MEdium Resolution Imaging Spectrometer onboard Envisat platform (MERIS/Envisat) were adapted for OLI/Landsat-8 and MSI/Sentinel-2A bands...
August 31, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28872679/potential-use-of-multispectral-imaging-technology-to-identify-moisture-content-and-water-holding-capacity-in-cooked-pork-sausages
#6
Fei Ma, Bin Zhang, Wu Wang, Peijun Li, Xiangli Niu, Conggui Chen, Lei Zheng
BACKGROUND: The traditional detection methods for moisture content (MC) and water-holding capacity (WHC) in cooked pork sausages (CPS) are destructive, time-consuming, and require skilled personnel and not suitable for on-line industry applications. The goal of this work was to explore the potential of multispectral imaging (MSI) in combination with multivariate analysis for the identification of MC and WHC in CPS. RESULTS: Spectra and textures of 156 CPS treated by six salt concentrations (0-2...
September 5, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28871184/motion-correction-in-optoacoustic-mesoscopy
#7
Mathias Schwarz, Natalie Garzorz-Stark, Kilian Eyerich, Juan Aguirre, Vasilis Ntziachristos
Raster-scan optoacoustic mesoscopy (RSOM), also termed photoacoustic mesoscopy, offers novel insights into vascular morphology and pathophysiological biomarkers of skin inflammation in vivo at depths unattainable by other optical imaging methods. Using ultra-wideband detection and focused ultrasound transducers, RSOM can achieve axial resolution of 4 micron and lateral resolution of 20 micron to depths of several millimeters. However, motion effects may deteriorate performance and reduce the effective resolution...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28863181/brca1-protein-expression-and-subcellular-localization-in-primary-breast-cancer-automated-digital-microscopy-analysis-of-tissue-microarrays
#8
Abeer M Mahmoud, Virgilia Macias, Umaima Al-Alem, Ryan J Deaton, Andre Kadjaksy-Balla, Peter H Gann, Garth H Rauscher
PURPOSE: Mutations in BRCA1 are associated with familial as well as sporadic aggressive subtypes of breast cancer, but less is known about whether BRCA1 expression or subcellular localization contributes to progression in population-based settings. METHODS: We examined BRCA1 expression and subcellular localization in invasive breast cancer tissues from an ethnically diverse sample of 286 patients and 36 normal breast tissue controls. Two different methods were used to label breast cancer tissues for BRCA1: (1) Dual immunofluoresent staining with BRCA1 and cytokeratin 8/18 and (2) immunohistochemical staining using the previously validated MS110 mouse monoclonal antibody...
2017: PloS One
https://www.readbyqxmd.com/read/28862663/novelty-detection-classifiers-in-weed-mapping-silybum-marianum-detection-on-uav-multispectral-images
#9
Thomas K Alexandridis, Afroditi Alexandra Tamouridou, Xanthoula Eirini Pantazi, Anastasia L Lagopodi, Javid Kashefi, Georgios Ovakoglou, Vassilios Polychronos, Dimitrios Moshou
In the present study, the detection and mapping of Silybum marianum (L.) Gaertn. weed using novelty detection classifiers is reported. A multispectral camera (green-red-NIR) on board a fixed wing unmanned aerial vehicle (UAV) was employed for obtaining high-resolution images. Four novelty detection classifiers were used to identify S. marianum between other vegetation in a field. The classifiers were One Class Support Vector Machine (OC-SVM), One Class Self-Organizing Maps (OC-SOM), Autoencoders and One Class Principal Component Analysis (OC-PCA)...
September 1, 2017: Sensors
https://www.readbyqxmd.com/read/28860899/photoacoustic-imaging-for-differential-diagnosis-of-benign-polyps-versus-malignant-polyps-of-the-gallbladder-a-preliminary-study
#10
Hee-Dong Chae, Jae Young Lee, Jin-Young Jang, Jin Ho Chang, Jeeun Kang, Mee Joo Kang, Joon Koo Han
OBJECTIVE: To investigate the feasibility of ex vivo multispectral photoacoustic (PA) imaging in differentiating cholesterol versus neoplastic polyps, and benign versus malignant polyps, of the gallbladder. MATERIALS AND METHODS: A total of 38 surgically confirmed gallbladder polyps (24 cholesterol polyps, 4 adenomas, and 10 adenocarcinomas) from 38 patients were prospectively included in this study. The surgical specimens were set on a gel pad immersed in a saline-filled container...
September 2017: Korean Journal of Radiology: Official Journal of the Korean Radiological Society
https://www.readbyqxmd.com/read/28858817/learning-low-rank-decomposition-for-pan-sharpening-with-spatial-spectral-offsets
#11
Shuyuan Yang, Kai Zhang, Min Wang
Finding accurate injection components is the key issue in pan-sharpening methods. In this paper, a low-rank pan-sharpening (LRP) model is developed from a new perspective of offset learning. Two offsets are defined to represent the spatial and spectral differences between low-resolution multispectral and high-resolution multispectral (HRMS) images, respectively. In order to reduce spatial and spectral distortions, spatial equalization and spectral proportion constraints are designed and cast on the offsets, to develop a spatial and spectral constrained stable low-rank decomposition algorithm via augmented Lagrange multiplier...
August 25, 2017: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/28841010/luminescent-carbon-dot-mimics-assembled-on-dna
#12
Ke Min Chan, Wang Xu, Hyukin Kwon, Anna M Kietrys, Eric T Kool
Nanometer-sized fragments of carbon in the form of multilayer graphene ("carbon dots") have been under highly active study for applications in imaging. While offering advantages of low toxicity and photostability, such nanomaterials are inhomogeneous and have limited wavelengths of emission. Here we address these issues by assembling luminescent aromatic C16-C38 hydrocarbons together on a DNA scaffold in homogeneous, soluble molecular compounds. Monomer deoxyribosides of five different aromatic hydrocarbons were synthesized and assembled into a library of 1296 different tetramer compounds on PEG-polystyrene beads...
September 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28836220/spectral-unmixing-methods-and-tools-for-the%C3%A2-detection-and-quantitation-of-collagen-and-other-macromolecules-in-tissue-specimens
#13
Zachary T Harmany, Farzad Fereidouni, Richard M Levenson
Collagen and other components in the extracellular matrix are proving of increasing importance for the understanding of complex cell and tissue interactions in a variety of settings. Detection and quantitation of these components can still prove challenging, and a number of techniques have been developed. We focus here on methods in fluorescence-based assessments, including multiplexed immunodetection and the use of simpler histochemical stains, both complemented by linear unmixing techniques. Typically, differentiating these components requires the use of a set of optical filters to isolate each fluorescent compound from each other and from often bright background autofluorescence signals...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28833734/modulation-of-multiscale-3d-lattices-through-conformational-control-painting-silk-inverse-opals-with-water-and-light
#14
Yu Wang, Daniele Aurelio, Wenyi Li, Peter Tseng, Zhaozhu Zheng, Meng Li, David L Kaplan, Marco Liscidini, Fiorenzo G Omenetto
Structural proteins from naturally occurring materials are an inspiring template for material design and synthesis at multiple scales. The ability to control the assembly and conformation of such materials offers the opportunity to define fabrication approaches that recapitulate the dimensional hierarchy and structure-function relationships found in nature. A simple and versatile directed assembly method of silk fibroin, which allows the design of structures across multiple dimensional scales by generating and tuning structural color in large-scale, macro defect-free colloidally assembled 3D nanostructures in the form of silk inverse opals (SIOs) is reported...
August 18, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28833448/a-chemical-shift-encoding-cse-approach-for-spectral-selection-in-fluorine-19-mri
#15
Kai D Ludwig, Diego Hernando, Nathan T Roberts, Ruud B van Heeswijk, Sean B Fain
PURPOSE: To develop a chemical shift encoding (CSE) approach for fluorine-19 MRI of perfluorocarbons in the presence of multiple known fluorinated chemical species. THEORY AND METHODS: A multi-echo CSE technique is applied for spectral separation of the perfluorocarbon perfluoro-15-crown-5-ether (PFCE) and isoflurane (ISO) based on their chemical shifts at 4.7 T. Cramér-Rao lower bound analysis is used to identify echo combinations with optimal signal-to-noise performance...
August 22, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28827758/in-vivo-label-free-structural-and-biochemical-imaging-of-coronary-arteries-using-an-integrated-ultrasound-and-multispectral-fluorescence-lifetime-catheter-system
#16
Julien Bec, Jennifer E Phipps, Dimitris Gorpas, Dinglong Ma, Hussain Fatakdawala, Kenneth B Margulies, Jeffrey A Southard, Laura Marcu
Existing clinical intravascular imaging modalities are not capable of accurate detection of critical plaque pathophysiology in the coronary arteries. This study reports the first intravascular catheter combining intravascular ultrasound (IVUS) with multispectral fluorescence lifetime imaging (FLIm) that enables label-free simultaneous assessment of morphological and biochemical features of coronary vessels in vivo. A 3.7 Fr catheter with a fiber-optic channel was constructed based on a 40 MHz clinical IVUS catheter...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28822291/highly-absorbing-multispectral-near-infrared-polymer-nanoparticles-from-one-conjugated-backbone-for-photoacoustic-imaging-and-photothermal-therapy
#17
Haobin Chen, Jian Zhang, Kaiwen Chang, Xiaoju Men, Xiaofeng Fang, Libo Zhou, Dongliang Li, Duyang Gao, Shengyan Yin, Xuanjun Zhang, Zhen Yuan, Changfeng Wu
Semiconducting polymers with specific absorption are useful in various applications, including organic optoelectronics, optical imaging, and nanomedicine. However, the optical absorption of a semiconducting polymer with a determined structure is hardly tunable when compared with that of inorganic semiconductors. In this work, we show that the optical absorption of polymer nanoparticles from one conjugated backbone can be effectively tuned through judicious design of the particle morphology and the persistence length of polymers...
August 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28821560/optoacoustic-detection-of-early-therapy-induced-tumor-cell-death-using-a-targeted-imaging-agent
#18
Bangwen Xie, Michal R Tomaszewski, Andre Neves, Susana Ros, De-En Hu, Sarah McGuire, Stefanie Roshy Mullins, David A Tice, Richard C A Sainson, Sarah E Bohndiek, Robert W Wilkinson, Kevin M Brindle
The development of new treatments and their deployment in the clinic may be assisted by imaging methods that allow an early assessment of treatment response in individual patients. The C2A domain of Synaptotagmin-I (C2Am), which binds to the phosphatidylserine (PS) exposed by apoptotic and necrotic cells, has been developed as an imaging probe for detecting cell death. Multispectral optoacoustic tomography (MSOT) is a real-time and clinically applicable imaging modality that was used here with a near infrared (NIR) fluorophore-labeled C2Am to image tumor cell death in mice treated with a TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2) agonist and with 5-fluorouracil (5-FU)...
August 18, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28819855/multispectral-imaging-analysis-of-circulating-tumor-cells-in-negatively-enriched-peripheral-blood-samples
#19
Brandon Miller, Maryam Lustberg, Thomas A Summers, Jeffrey J Chalmers
A variety of biomarkers are present on cells in peripheral blood of patients with a variety of disorders, including solid tumor malignancies. While rare, characterization of these cells for specific protein levels with the advanced technology proposed, will lead to future validation studies of blood samples as "liquid biopsies" for the evaluation of disease status and therapeutic response. While circulating tumor cells (CTCs) have been isolated in the blood samples of patients with solid tumors, the exact role of CTCs as clinically useful predictive markers is still debated...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28815503/imaging-neural-architecture-in-brainbow-samples
#20
Douglas H Roossien, Dawen Cai
The fluorescent protein revolution has made the light microscope the most widely used tool for studying biological structure from the single-molecule to whole organism scales. However, traditional approaches are limited in their ability to resolve components in highly complex structures, such as the brain. In recent years, this limitation has been circumvented by the development of multicolor labeling methods, termed Brainbow. Brainbow tools rely on site-specific recombinases to make stochastic "choices" between different combinations of fluorescent proteins so that structures in close proximity to one another can be resolved based on their color profile...
2017: Methods in Molecular Biology
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