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https://www.readbyqxmd.com/read/28728551/reduced-glutathione-and-glutathione-disulfide-in-the-blood-of-glucose-6-phosphate-dehydrogenase-deficient-newborns
#1
Zhen-Hua Gong, Guo-Li Tian, Qi-Wei Huang, Yan-Min Wang, Hong-Ping Xu
BACKGROUND: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly detected during mass screening for neonatal disease. We developed a method to measure reduced glutathione (GSH) and glutathione disulfide (GSSG) using tandem mass spectrometry (MS/MS) for detecting G6PD deficiency. METHODS: The concentration of GSH and the GSH/GSSG ratio in newborn dry-blood-spot (DBS) screening and in blood plus sodium citrate for test confirmation were examined by MS/MS using labeled glycine as an internal standard...
July 20, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28721814/refractory-causes-of-kernicterus-in-developed-countries-can-we-eradicate-g6pd-deficiency-triggered-and-low-bilirubin-kernicterus
#2
Jon Freeman Watchko
BACKGROUND: Glucose-6-phosphate dehydrogenase (G6PD) deficiency triggered and low-bilirubin kernicterus persist despite current prevention strategies. OBJECTIVE: Efforts to eradicate bilirubin induced brain injury in these two conditions will require novel approaches to riskassessment and hyperbilirubinemia evaluation. METHOD: In the case of G6PD deficency, a heightenedawareness of populations at risk on who expanded kernicterus preventio strategies can befocused including intensified parental engagement, education and counselling on neonataljaundice...
July 18, 2017: Current Pediatric Reviews
https://www.readbyqxmd.com/read/28699887/upward-trend-of-dapsone-induced-methemoglobinemia-in-renal-transplant-community%C3%A2
#3
Sohail Abdul Salim, Lakshmi Ramachandran Nair, Venkataraman Palabindala, Iasmina Craici
Dapsone, a sulfone antibiotic, is used for prophylaxis of Pneumocystis jirovecii pneumonia in patients with documented sulfa allergy. Acquired methemoglobinemia caused by dapsone is not uncommon in patients with normal glucose-6-phosphate dehydrogenase (G6PD) levels. Discrepancy between oxygen saturation measured by pulse oximetry and arterial oxygen saturation (SpO2) readings, a phenomenon known as "saturation gap," is noted with cases of methemoglobinemia. Although its prevalence in renal transplant patients is poorly described, there is evidence that its incidence is increasing...
July 12, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28699877/isolation-and-characterization-of-kingella-negevensis-sp-nov-a-novel-kingella-species-detected-in-a-healthy-paediatric-population
#4
Nawal El Houmami, Sofiane Bakour, Janek Bzdrenga, Jaishiram Rathored, Hervé Seligmann, Catherine Robert, Nicholas Armstrong, Jacques Schrenzel, Didier Raoult, Pablo Yagupsky, Pierre-Edouard Fournier
We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28693459/screening-for-glucose-6-phosphate-dehydrogenase-deficiency-in-neonates-a-comparison-between-cord-and-peripheral-blood-samples
#5
Saif AlSaif, Ma Bella Ponferrada, Khalid AlKhairy, Khalil AlTawil, Adel Sallam, Ibrahim Ahmed, Mohammed Khawaji, Khalid AlHathlol, Beverly Baylon, Ahmed AlSuhaibani, Mohammed AlBalwi
BACKGROUND: The use of cord blood in the neonatal screening for glucose-6-phosphate dehydrogenase (G6PD) deficiency is being done with increasing frequency but has yet to be adequately evaluated against the use of peripheral blood sample which is usually employed for confirmation. We sought to determine the incidence and gender distribution of G6PD deficiency, and compare the results of cord against peripheral blood in identifying G6PD DEFICIENCY neonates using quantitative enzyme activity assay...
July 11, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28692052/disrupting-g6pd-mediated-redox-homeostasis-enhances-chemosensitivity-in-colorectal-cancer
#6
H-Q Ju, Y-X Lu, Q-N Wu, J Liu, Z-L Zeng, H-Y Mo, Y Chen, T Tian, Y Wang, T-B Kang, D Xie, M-S Zeng, P Huang, R-H Xu
Glucose-6-phosphate dehydrogenase (G6PD) is a key enzyme that generates NADPH to maintain reduced glutathione (GSH), which scavenges reactive oxygen species (ROS) to protect cancer cell from oxidative damage. In this study, we mainly investigate the potential roles of G6PD in colorectal cancer (CRC) development and chemoresistance. We discover that G6PD is overexpressed in CRC cells and patient specimens. High expression of G6PD predicts poor prognosis and correlated with poor outcome of oxaliplatin-based first-line chemotherapy in patients with CRC...
July 10, 2017: Oncogene
https://www.readbyqxmd.com/read/28676055/malaria-in-brazil-colombia-peru-and-venezuela-current-challenges-in-malaria-control-and-elimination
#7
REVIEW
Judith Recht, André M Siqueira, Wuelton M Monteiro, Sonia M Herrera, Sócrates Herrera, Marcus V G Lacerda
In spite of significant progress towards malaria control and elimination achieved in South America in the 2000s, this mosquito-transmitted tropical disease remains an important public health concern in the region. Most malaria cases in South America come from Amazon rain forest areas in northern countries, where more than half of malaria is caused by Plasmodium vivax, while Plasmodium falciparum malaria incidence has decreased in recent years. This review discusses current malaria data, policies and challenges in four South American Amazon countries: Brazil, Colombia, Peru and the Bolivarian Republic of Venezuela...
July 4, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28670947/genetic-background-of-the-sickle-cell-disease-pediatric-population-of-dakar-senegal-and-characterization-of-a-novel-frameshift-%C3%AE-thalassemia-mutation-hbb-c-265_266del-p-leu89glufs-2
#8
Fatou Gueye Tall, Cyril Martin, El Hadji Malick Ndour, Indou Déme Ly, Céline Renoux, Louis Chillotti, Nicolas Veyrenche, Philippe Connes, Papa Madieye Gueye, Rokhaya Ndiaye Diallo, Philippe Lacan, Ibrahima Diagne, Pape Amadou Diop, Aynina Cissé, Philomène Lopez Sall, Philippe Joly
Sickle cell disease is a genetic disorder with a large variability in the pattern and severity of clinical manifestations. Different genetic modulators have been identified but very few epidemiologic data are available on these modifier genes in Senegal. This study aimed to determine their prevalence in a Senegalese sickle cell disease pediatric population. The following genetic parameters were genotyped in 295 sickle cell disease children of the Dakar pediatric hospital: sickle cell disease genotype [β(S)/β(S) (HBB: c...
March 2017: Hemoglobin
https://www.readbyqxmd.com/read/28670638/a-comparison-of-rapid-diagnostic-testing-by-plasmodium-lactate-dehydrogenase-and-quantitative-buffy-coat-technique-in-malaria-diagnosis-in-children
#9
Ifeanyi Kanayo Ifeorah, Biobele J Brown, Olugbemiro O Sodeinde
BACKGROUND: The World Health Organization (WHO) considers early and rapid diagnosis as one of the strategies to control malaria. This study compared the performance of Quantitative Buffy Coat (QBC) test and the Plasmodium lactate dehydrogenase (pLDH) rapid diagnostic test (RDT) with microscopy as the gold standard. MATERIALS AND METHODS: The study involved children ages 0-5 years who presented with a history of fever at the University College Hospital, Ibadan, Nigeria...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28665548/effect-of-astaxanthin-and-aluminum-chloride-on-erythrocyte-g6pd-and-6pgd-enzyme-activities-in-vivo-and-on-erythrocyte-g6pd-in-vitro-in-rats
#10
Yusuf Temel, Aydın Şükrü Bengü, Hurrem Turan Akkoyun, Mahire Akkoyun, Mehmet Ciftci
In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2',5'-ADP-Sepharose 4B affinity gel. The effects of Ast and Al(3+) ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al(3+) inhibited the enzyme activity noncompetitively (IC50 values; 0...
June 30, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28656087/toxicology-effects-of-berberis-vulgaris-barberry-and-its-active-constituent-berberine-a-review
#11
REVIEW
Seyede Zohre Kamrani Rad, Maryam Rameshrad, Hossein Hosseinzadeh
Berberis vulgaris and berberine, its main component, traditionally have been used for treatment of various disorders. The pharmacological properties of them have been investigated using different in vivo and in vitro models. In spite of beneficial effects of B. vulgaris on different cell lines, there are documents have revealed negative impacts of it on animal and human. In this regards, the determination of its toxicity in a scientific view is necessary. In current report, we provide classified information about the toxicity of B...
May 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28644754/expression-of-enzymes-related-to-glucose-metabolism-in-non-small-cell-lung-cancer-and-prognosis
#12
Alexandra Giatromanolaki, Efthimios Sivridis, Stella Arelaki, Michael I Koukourakis
Purpose/Aim: Cancer cells are addicted to glycolytic anaerobic pathways, in presence or in absence of a functional Krebs' cycle (phenomenon Warburg). This metabolic predilection relies on both extracellular (impaired vascularization and oxygenation) and intracellular (oncogenic activation of genes) causes. MATERIALS AND METHODS: We investigated the expression and prognostic relevance of enzymes involved in the glucose absorption and metabolism, monocarboxylate transporter (MCT) expression, MCT1 and MCT2, pentose pathway (Glucose-6-phospahte dehydrogenase G6PD), glycogene synthesis (glycogene synthase GYS1), glycolysis (Hexokinase HXKII, phosphofructokinase PFK1, fructose biphosphate aldolase), fate of pyruvate (pyruvate dehydrogenase PDH, phosphorylated pPDH, PDH kinase PDK1, lactate dehydrogenase LDH5 and LDH1) and key Kreb's cycle enzymes (citrate synthase CSynth and isocitrate dehydrogenase IDH)...
June 23, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/28627690/data-mining-and-pathway-analysis-of-glucose-6-phosphate-dehydrogenase-with-natural-language-processing
#13
Long Chen, Chunhua Zhang, Yanling Wang, Yuqian Li, Qiaoqiao Han, Huixin Yang, Yuechun Zhu
Human glucose-6-phosphate dehydrogenase (G6PD) is a crucial enzyme in the pentose phosphate pathway, and serves an important role in biosynthesis and the redox balance. G6PD deficiency is a major cause of neonatal jaundice and acute hemolyticanemia, and recently, G6PD has been associated with diseases including inflammation and cancer. The aim of the present study was to conduct a search of the National Center for Biotechnology Information PubMed library for articles discussing G6PD. Genes that were identified to be associated with G6PD were recorded, and the frequency at which each gene appeared was calculated...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28624280/technical-note-evaluation-of-endogenous-control-gene-expression-in-bovine-neutrophils-by-reverse-transcription-quantitative-pcr-using-microfluidics-gene-expression-arrays
#14
M A Crookenden, C G Walker, B Kuhn-Sherlock, A Murray, V S R Dukkipati, A Heiser, J R Roche
Reverse-transcription quantitative-PCR (RT-qPCR) is commonly used for assessing the cellular response to changes in physiologic and pathologic conditions. The selection of stable endogenous control genes is an important step of any RT-qPCR study, as expression can vary depending on the experimental environment. Our objective was to identify endogenous control genes in circulating neutrophils isolated from cows during the peripartum period. To do this, we used microfluidics gene expression arrays (Fluidigm, San Francisco, CA) for RT-qPCR analysis...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28619120/g6pd-deficiency-alleles-in-a-malaria-endemic-region-in-the-western-brazilian-amazon
#15
Jamille G Dombrowski, Rodrigo M Souza, Jonathan Curry, Laura Hinton, Natercia R M Silva, Lynn Grignard, Ligia A Gonçalves, Ana Rita Gomes, Sabrina Epiphanio, Chris Drakeley, Jim Huggett, Taane G Clark, Susana Campino, Claudio R F Marinho
BACKGROUND: Plasmodium vivax parasites are the predominant cause of malaria infections in the Brazilian Amazon. Infected individuals are treated with primaquine, which can induce haemolytic anaemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals and may lead to severe and fatal complications. This X-linked disorder is distributed globally and is caused by allelic variants with a geographical distribution that closely reflects populations exposed historically to endemic malaria...
June 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28605472/safety-and-efficacy-of-adding-a-single-low-dose-of-primaquine-to-the-treatment-of-adult-patients-with-plasmodium-falciparum-malaria-in-senegal-to-reduce-gametocyte-carriage-a-randomized-controlled-trial
#16
Roger C Tine, Khadime Sylla, Babacar T Faye, Eugenie Poirot, Fatou B Fall, Doudou Sow, Duolao Wang, Magatte Ndiaye, Jean Louis Ndiaye, Babacar Faye, Brian Greenwood, Oumar Gaye, Paul Milligan
Introduction.: More information is needed about the safety of low-dose primaquine in populations where G6PD deficiency is common. Methods.: Adults with Plasmodium falciparum malaria were randomized to receive 1 of 3 artemisinin combination therapies (ACTs) with or without primaquine (0.25 mg/kg). Glucose-6-phosphate dehydrogenase (G6PD) status was determined using a rapid test. Patients were followed for 28 days to record hemoglobin concentration, adverse events, and gametocyte carriage...
June 12, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28597476/safe-fluoroquinolones-prophylaxis-in-blood-cancer-patients-with-chemotherapy-induced-neutropenia-and-glucose-6-phosphate-dehydrogenase-deficiency
#17
M Sanna, G Caocci, F Orrù, A Ledda, A Vacca, E Piras, C Fozza, P Deias, G Tidore, F Dore, G La Nasa
WHAT IS KNOWN AND OBJECTIVE: Bacterial infections are the leading causes of morbidity and mortality in haematologic patients with chemotherapy-induced neutropenia. The only strategy shown to be effective in reducing febrile neutropenia incidence is fluoroquinolone prophylaxis, but the safety of this class of drugs in patients with glucose-6-phosphate dehydrogenase deficiency (G6PD-), the most common human enzyme defect, is still controversial because of the claimed association with acute haemolytic anaemia...
June 8, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28596515/characterization-of-a-new-murine-cell-line-of-sarcomatoid-hepatocellular-carcinoma-and-its-application-for-biomarker-therapy-development
#18
Chia-Hung Yen, Chih-Chung Lai, Chen-Chung Liao, Sheng-Fan Wang, Yi-Jen Liao, Chien-Yi Tung, Jung-Hsien Hung, Shiu-Feng Huang, Yi-Ming Arthur Chen
Sarcomatoid hepatocellular carcinoma (SHC) is a rare type of HCC with significantly poorer survival than ordinary HCC. Little is known about the mechanism associated with SHC and its biomarkers and therapy. Here, we established a mouse liver cancer cell line and designated as Ymac-1. A sarcomatous appearance was observed in the allograft tumor arose from Ymac-1. Liver-secreted plasma proteins were found in Ymac-1 cultured supernatant by proteomics analysis. The positive staining of CK7, CK8, Vimentin and the suppressed expression of AFP indicated that Ymac-1 is a SHC cell line...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28591790/asian-g6pd-mahidol-reticulocytes-sustain-normal-plasmodium-vivax-development
#19
Germana Bancone, Benoit Malleret, Rossarin Suwanarusk, Nongnud Chowwiwat, Cindy S Chu, Rose McGready, Laurent Rénia, François Nosten, Bruce Russell
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzymatic disorder in humans and appears to be protective against falciparum severe malaria. Controversially, it is also thought that Plasmodium vivax has driven the recent selection of G6PD alleles. We use an experimental approach to determine whether G6PD-MahidolG487A variant, a widespread cause of severe G6PD deficiency in Southeast Asia, provides a barrier against vivax malaria. Our results show that the immature reticulocytes (CD71+) targeted by P...
June 7, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28583873/a-trade-off-between-catalytic-activity-and-protein-stability-determines-the-clinical-manifestations-of-glucose-6-phosphate-dehydrogenase-g6pd-deficiency
#20
Usa Boonyuen, Kamonwan Chamchoy, Thitiluck Swangsri, Thanyaphorn Junkree, Nicholas P J Day, Nicholas J White, Mallika Imwong
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common polymorphism and enzymopathy in humans, affecting approximately 400 million people worldwide. It is responsible for various clinical manifestations, including favism, hemolytic anemia, chronic non-spherocytic hemolytic anemia, spontaneous abortion, and neonatal hyperbilirubinemia. Understanding the molecular mechanisms underlying the severity of G6PD deficiency is of great importance but that of many G6PD variants are still unknown. In this study, we report the construction, expression, purification, and biochemical characterization in terms of kinetic properties and stability of five clinical G6PD variants-G6PD Bangkok, G6PD Bangkok noi, G6PD Songklanagarind, G6PD Canton+Bangkok noi, and G6PD Union+Viangchan...
June 3, 2017: International Journal of Biological Macromolecules
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