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N D B Ehelepola, A N Abayagunawardana, T N Sudusinghe
BACKGROUND: Hemolysis can occur in people with G6PD deficiency under oxidative stress. Acalypha indica is a tropical plant used as a medicinal plant as well as a vegetable. There are a few reported cases of Acalypha indica ingestion induced hemolysis in G6PD deficient people. All except one of them are from Sri Lanka. The information available at present (2017) about G6PD deficiency prevalence and variants of the G6PD gene among Sri Lankans is very sparse. There are no past reports on hemolytic crisis in a G6PD deficient person presenting mimicking leptospirosis...
March 14, 2018: BMC Research Notes
Aastha Chhabra, Shalini Mishra, Gaurav Kumar, Asheesh Gupta, Gaurav Kumar Keshri, Brij Bharti, Ram Niwas Meena, Amit Kumar Prabhakar, Dinesh Kumar Singh, Kalpana Bhargava, Manish Sharma
Hydrogen Sulfide (H2 S), recently identified as the third endogenously produced gaseous messenger, is a promising therapeutic prospect for multiple cardio-pathological states, including myocardial hypertrophy. The molecular niche of H2 S in normal or diseased cardiac cells is, however, sparsely understood. Here, we show that β-adrenergic receptor (β-AR) overstimulation, known to produce hypertrophic effects in cardiomyocytes, rapidly decreased endogenous H2 S levels. The preservation of intracellular H2 S levels under these conditions strongly suppressed hypertrophic responses to adrenergic overstimulation, thus suggesting its intrinsic role in this process...
December 2018: Cell Death Discovery
Aaron Leong, Eleanor Wheeler
Glycated hemoglobin (HbA1c) measures the amount of glucose in the blood in the previous 2-3 months and is used to test whether an individual has diabetes (HbA1c≥6.5%), or how well they are managing their diabetes. Genome-wide association studies have successfully identified multiple genomic loci influencing HbA1c, through both glycemic (factors that affect the amount blood glucose levels) and erythrocytic (factors that affect the red blood cell) pathways. Inaccuracies in HbA1c, due to non-glycemic variants, could lead to suboptimal care or adverse health consequences...
March 6, 2018: Current Opinion in Genetics & Development
Ferania Mella, Yasuhiro Yamashiro, Chris Adhiyanto, Tatehiko Tanaka, Takenori Nitta, Yuki Amao, Masafumi Kimoto
We report a new hemoglobin (Hb) variant, Hb Hachioji (HBB: c.187C>T), which was detected in a 32-year-old male with hemolytic anemia. The proband had undergone splenectomy in his childhood after being diagnosed with hereditary spherocytosis (HS) with no clinical improvement. A recent study showed that Heinz bodies were frequently observed in his red cells, however, no abnormal band was separated by isoelectric focusing (IEF), and the isopropanol (instability) test was negative. Direct sequencing revealed that the proband was a heterozygous carrier of a novel mutation (GCT>GTT) at codon 62 of the β-globin gene, leading to an alanine to valine substitution...
March 7, 2018: Hemoglobin
Yusuke Yamamoto, Kohkichi Hosoda, Taichiro Imahori, Jun Tanaka, Kazuya Matsuo, Tomoaki Nakai, Yasuhiro Irino, Masakazu Shinohara, Naoko Sato, Takashi Sasayama, Kazuhiro Tanaka, Hiroaki Nagashima, Masaaki Kohta, Eiji Kohmura
Molecular mechanism underlying ischemic stroke remains poorly understood. We previously reported glucose 6-phosphate dehydrogenase (G6PD) activity in pentose phosphate pathway (PPP) is activated via heat shock protein 27 (HSP27) phosphorylation at serine 85 (S85) by ataxia telangiectasia mutated (ATM) kinase during cerebral ischemia. This mechanism seems to be endogenous antioxidative system. To determine whether this system also works during reperfusion, we performed comparative metabolic analysis of reperfusion effect on metabolism in rat cortex using middle cerebral artery occlusion (MCAO)...
March 3, 2018: Brain Research
Xiangchou Yang, Haihao Ye, Muqing He, Xiaohai Zhou, Ni Sun, Wenjian Guo, Xiaoji Lin, He Huang, Ying Lin, Rongxin Yao, Hong Wang
Multiple myeloma (MM), the second most common hematologic malignancy, is an incurable disease characterized by the accumulation of malignant plasma cells within the bone marrow. Though great progresses have been made in understanding the mechanisms of MM, metabolic plasticity and drug resistance remain largely unknown. In this study, we found lncRNA Protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P) is highly expressed in MM and is associated with the survival rate of MM patients. PDIA3P regulates MM growth and drug resistance through Glucose 6-phosphate dehydrogenase (G6PD) and the pentose phosphate pathway (PPP)...
March 1, 2018: Biochemical and Biophysical Research Communications
Alexandra Rehfuss, Joseph Mahon, Igor Sorokin, Cynthia Smith, Barry S Stein
OBJECTIVE: To identify difficult to see ureteral orifices (UOs), urologists need a method to stain the urine. Phenazopyridine, a urinary analgesic which discolors the urine orange, can be administered orally pre-operatively. We evaluated the usefulness of phenazopyridine in identifying the UOs and optimal timing of administration. METHODS: Adult patients undergoing endoscopic procedures at the Stratton VA were prospectively enrolled. Preoperative metabolic panels were reviewed...
March 1, 2018: Urology
Cindy S Chu, Germana Bancone, François Nosten, Nicholas J White, Lucio Luzzatto
Oxidative agents can cause acute haemolytic anaemia in persons with G6PD deficiency. Understanding the relationship between G6PD genotype and the phenotypic expression of the enzyme deficiency is necessary so that severe haemolysis can be avoided. The patterns of oxidative haemolysis have been well described in G6PD deficient hemizygous males and homozygous females; and haemolysis in the proportionally more numerous heterozygous females has been documented mainly following consumption of fava beans and more recently dapsone...
March 2, 2018: Malaria Journal
Milan Talwar, Sriram Krishnamurthy, Narayanan Parameswaran, C G Delhikumar, Satish Haridasan, Bheemanathi Hanuman Srinivas
Glucose-6-phosphate dehydrogenase (G6PD) deficiency leading to acute intravascular haemolysis and acute kidney injury (AKI) is a known clinical presentation. However, there is a paucity of information regarding the occurrence of rhabdomyolysis and myoglobinuria in G6PD-deficient individuals, especially children. An 11-year-old south Indian Tamil girl presented with severe anaemia and anuric AKI following a short febrile illness. Investigations demonstrated evidence of intravascular haemolysis and rhabdomyolysis, and on histopathology myoglobin deposits (casts) were detected in the renal tubules...
March 1, 2018: Paediatrics and International Child Health
Musa O Babalola, Ngozi A Imaga, Titilola A Samuel, Iyanu P Diriwari, Olajumoke Kolade, Irene Ezeamalu, Adefioye O Laoye, Oyesola O Ojewunmi
Glucose-6-phosphate dehydrogenase (G6PD) is an essential enzyme in the pentose phosphate pathway that prevents oxidative damage to cells. This study determined the genotypic and allelic frequencies of G6PD G202A and A376G and also investigated correlation between G6PD polymorphisms and hemoglobin (Hb) phenotypes in children in Lagos, Nigeria. Seventy-eight children [55 with Hb AA (βΑ /βA ) and 23 with Hb AS (βΑ /βS ) trait] and 65 Hb SS (βS /βS ) (HBB: c.20A>T) subjects in steady state with age range between 5-15 years were recruited for the study...
March 1, 2018: Hemoglobin
Amre Nasr, Ayman M Saleh, Muna Eltoum, Amir Abushouk, Anhar Hamza, Ahmad Aljada, Mohamed E El-Toum, Yousif A Abu-Zeid, Gamal Allam, Gehad ElGhazali
Fulani and Masaleit are two sympatric ethnic groups in western Sudan who are characterised by marked differences in susceptibility to Plasmodium falciparum malaria. It has been demonstrated that Glucose-6-phosphate dehydrogenase (G6PD) deficiency and Sickle cell trait HbAS carriers are protected from the most severe forms of malaria. This study aimed to investigate a set of specific IgG subclasses against P. falciparum Apical Membrane Antigen 1 (AMA-1 3D7), haemoglobin variants and (G6PD) in association with malaria susceptibility among Fulani ethnic group compared to sympatric ethnic group living in Western Sudan...
February 24, 2018: Acta Tropica
Ming Lu, Lu Lu, Qiongzhu Dong, Guangyang Yu, Jinhong Chen, Lunxiu Qin, Lianxin Wang, Wenwei Zhu, Huliang Jia
Altered metabolism is one of the hallmarks of cancer cells. Pentose phosphate pathway (PPP) is a fundamental component of cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the PPP, is elevated in many cancers and contributes to tumor growth by producing ribose-5-phosphate and NADPH through PPP. However, the role of G6PD in hepatocellular carcinoma (HCC) metastasis and the clinical significance of G6PD in HCC progression and prognosis have not been well determined. In this study, by investigating tissue samples from HCC patients and HCC cell lines, we found that elevated G6PD expression is significantly associated with HCC metastasis and poor prognosis of HCCs, and that knockdown of G6PD inhibits in vitro proliferation, migration and invasion of HCC cell lines...
February 17, 2018: Acta Biochimica et Biophysica Sinica
Valentina Gallo, Oleksii A Skorokhod, Luigi Felice Simula, Tiziana Marrocco, Elisa Tambini, Evelin Schwarzer, Pascal Marget, Gérard Duc, Paolo Arese
No abstract text is available yet for this article.
February 21, 2018: Blood
Carolina Castro, Amalia Peréz-Jiménez, Filipe Coutinho, Geneviève Corraze, Stéphane Panserat, Helena Peres, Aires Oliva Teles, Paula Enes
The aim of the present study was to assess the impact of an acute handling stress on hepatic oxidative status of European sea bass (Dicentrarchus labrax) juveniles fed diets differing in lipid so urce and carbohydrate content. For that purpose, four diets were formulated with fish oil (FO) and vegetable oils (VO) as lipid source and with 20 or 0% gelatinized starch as carbohydrate source. Triplicate groups of fish with 74 g were fed each diet during 13 weeks and then subjected to an acute handling stress. Stress exposure decreased hematocrit (Ht) and hemoglobin (Hb) levels...
February 19, 2018: Fish Physiology and Biochemistry
Vassilis L Tzounakas, Federica Gevi, Hara T Georgatzakou, Lello Zolla, Issidora S Papassideri, Anastasios G Kriebardis, Sara Rinalducci, Marianna H Antonelou
Objective: Transfusion of fresh frozen plasma (FFP) helps in maintaining the coagulation parameters in patients with acquired multiple coagulation factor deficiencies and severe bleeding. However, along with coagulation factors and procoagulant extracellular vesicles (EVs), numerous bioactive and probably donor-related factors (metabolites, oxidized components, etc.) are also carried to the recipient. The X-linked glucose 6-phosphate dehydrogenase deficiency (G6PD- ), the most common human enzyme genetic defect, mainly affects males...
2018: Frontiers in Medicine
A Khanam, Q S Akter, F Karim, M R Zannat
The patient with diabetes mellitus (DM) and its complication is increasing in our country and all over the world. There is also significant increase in the prevalence of erythrocyte glucose-6-phosphate dehydrogenase (eG6PD) deficiency in the men with longer duration of diabetes mellitus. To assess the eG6PD in type 2 diabetes male subjects to observe their enzyme status. This cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka, Bangladesh from January 2011 to December 2011...
January 2018: Mymensingh Medical Journal: MMJ
Parul Mehta, Ramakant Kaushik, Karn Pratap Singh, Ankur Sharma, Manoj Kumar Singh, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik
The aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (SCNT). The gene construct was delivered into buffalo fetal fibroblasts (BFF) by nucleofection following which, the transfected cells were selected by culture in the presence of G418 for 3 weeks...
February 15, 2018: Cellular Reprogramming
Weipeng Hong, Peiheng Cai, Chuncao Xu, Di Cao, Weibang Yu, Zhongxiang Zhao, Min Huang, Jing Jin
The pentose phosphate pathway (PPP), which branches from glycolysis, is correlated with cancer cell proliferation, survival and senescence. In this study, differences in the metabolic profile of the PPP and the redox status of human lung carcinoma A549 cells and cisplatin-induced multidrug-resistant A549/DDP cells were analyzed and evaluated. The results showed that A549/DDP cells exhibited differential PPP-derived metabolic features and redox-related molecules. A549/DDP cells exhibited increased expression and enzymatic activity of PPP enzyme glucose-6-phosphate dehydrogenase (G6PD)...
2018: Frontiers in Pharmacology
Aleix Elizalde-Torrent, Fernando Val, Ingrid Cardoso C Azevedo, Wuelton M Monteiro, Luiz C L Ferreira, Carmen Fernández-Becerra, Hernando A Del Portillo, Marcus V G Lacerda
BACKGROUND: Splenomegaly is one of the most common features of malaria. However, spontaneous splenic rupture, although unusual, represents a severe complication often leading to death. It is mostly seen in acute infection and primary attack, and it is most commonly associated with Plasmodium vivax. Here, a case of spontaneous splenic rupture diagnosed with a portable ultrasound apparatus shortly after starting treatment and with recurrent parasitaemia after splenectomy, is reported. CASE DESCRIPTION: In November 2015, a 45-year-old Brazilian man presented to the hospital in Manaus with fever, headache and myalgia...
February 13, 2018: Malaria Journal
Guankui Du, Man Xiao, Xiuyu Wei, Chen Zhou, Shuoshuo Li, Wangwei Cai
Favism is an acute hemolytic syndrome caused by the ingestion of fava bean (FB) in glucose 6-phosphate dehydrogenase (G6PD) deficient individuals. However, little is known about the global transcripts alteration in liver tissue after FB ingestion in G6PD-normal and -deficient states. In this study, deep sequencing was used to analyze liver genes expression alterations underlying the effects of FB in C3H (Wild Type, WT) and G6PD-deficient (G6PDx) mice and to evaluate and visualize the collective annotation of a list of genes to Gene Ontology (GO) terms associated with favism...
February 8, 2018: Gene
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