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https://www.readbyqxmd.com/read/29163204/the-effect-of-being-aerobically-active-vs-inactive-on-cutaneous-vascular-conductance-during-local-heat-stress-in-an-older-population
#1
Ulrike H Mitchell, Samantha Burton, Christopher Gordon, Gary W Mack
Objective: To test the hypothesis that long- term aerobically trained elderly individuals have a greater amount of bioavailable nitric oxide (NO) and have a larger cutaneous vasodilation during local heat stress compared to their inactive elderly counterparts. Methods: Eight aerobically trained and 8 inactive older men (>60 years old) participated in this study. NO bioavailability in blood and intradermal dialysate were measured with an ozone based chemiluminescence NO analyzer. Cutaneous vasodilator response to local heating was obtained using laser Doppler velocimetry...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29145844/development-and-evaluation-of-an-interferon-gamma-assay-for-the-diagnosis-of-tuberculosis-in-red-deer-experimentally-infected-with-mycobacterium-bovis
#2
María Ángeles Risalde, Jobin Thomas, Iker Sevilla, Miriam Serrano, Jose Antonio Ortíz, Joseba Garrido, Mercedes Domínguez, Lucas Domínguez, Christian Gortázar, Jose Francisco Ruíz-Fons
BACKGROUND: Red deer (Cervus elaphus) is regarded as an epidemiologically relevant host for Mycobacterium bovis (M. bovis) and closely related members of the Mycobacterium tuberculosis complex that cause animal tuberculosis (TB). The standard antemortem screening test for the detection of TB in deer is the intradermal tuberculin skin test, but the detection of interferon-gamma (IFNγ) produced by white blood cells exposed to M. bovis antigens can be used as an alternative or supplemental assay in most TB eradication/control programs...
November 16, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29140982/development-standardization-and-testing-of-a-bacterial-wound-infection-model-based-on-ex-vivo-human-skin
#3
Christoph Schaudinn, Christin Dittmann, Jana Jurisch, Michael Laue, Nazende Günday-Türeli, Ulrike Blume-Peytavi, Annika Vogt, Fiorenza Rancan
Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using-otherwise discarded-skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis...
2017: PloS One
https://www.readbyqxmd.com/read/29138430/extracellular-vesicles-derived-from-mscs-activates-dermal-papilla-cell-in-vitro-and-promotes-hair-follicle-conversion-from-telogen-to-anagen-in-mice
#4
Ramya Lakshmi Rajendran, Prakash Gangadaran, Soon Sun Bak, Ji Min Oh, Senthilkumar Kalimuthu, Ho Won Lee, Se Hwan Baek, Liya Zhu, Young Kwan Sung, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Hair loss is a common medical problem. In this study, we investigated the proliferation, migration, and growth factor expression of human dermal papilla (DP) cells in the presence or absence of treatment with mesenchymal stem cell extracellular vesicles (MSC-EVs). In addition, we tested the efficacy of MSC-EV treatment on hair growth in an animal model. MSC-EV treatment increased DP cell proliferation and migration, and elevated the levels of Bcl-2, phosphorylated Akt and ERK. In addition; DP cells treated with MSC-EVs displayed increased expression and secretion of VEGF and IGF-1...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137399/molecular-characteristics-and-serodiagnostic-potential-of-chitinase-like-protein-from-sarcoptes-scabiei
#5
Ran He, Nengxing Shen, Haojie Zhang, Yongjun Ren, Manli He, Jing Xu, Cheng Guo, Yue Xie, Xiaobin Gu, Weimin Lai, Xuerong Peng, Guangyou Yang
Scabies, caused by the mite Sarcoptes scabiei, is an allergic skin disease that affects millions of people and other mammals worldwide. This highly contagious parasitic disease is among the top 50 epidemic disease and is regarded as a neglected tropical disease. Diagnosis of scabies is difficult in the early stage, and the pathogenesis of scabies is not currently clear. Here, we expressed, identified and located the chitinase-like protein of S. scabiei (SsCLP), and evaluated its potential as an early-stage diagnostic antigen for rabbit scabies...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29114488/seroprevalence-of-human-cystic-echinococcosis-from-north-india-2004-2015
#6
Kamran Zaman, Abhishek Mewara, Sunil Kumar, Kapil Goyal, Sumeeta Khurana, Praveen Tripathi, Rakesh Sehgal
Context: Cystic echinococcosis (CE) caused by Echinococcus granulosus is a disease of a significant burden in India. The World Health Organization recommends the use of hospital data for population surveillance to measure the prevalence of CE. Aim: The aim of this study was to estimate the seroprevalence of CE and to compare with previous prevalence rates to estimate the changing pattern in seroprevalence of CE. Materials and Methods: A retrospective analysis of laboratory data of 3929 clinically and/or radiologically suspected cases of CE was carried out for 12 years from 2004 to 2015 and compared to the previous data from 1984 to 2003...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29111148/effects-of-opioid-and-nonopioid-analgesics-on-canine-wheal-formation-and-cultured-human-mast-cell-degranulation
#7
Eric Schmidt-Rondon, Zhenping Wang, Shelle A Malkmus, Anna Di Nardo, Keith Hildebrand, Linda Page, Tony L Yaksh
Mast cell (MC) degranulation has been implicated in the side effect profile of a variety of clinically useful agents. Thus, after intrathecal delivery, formation of space-occupying, meningeally-derived masses may be related to local MC degranulation. We systematically characterized degranulating effects of opioid and nonopioid analgesics on cutaneous flares in the dog and in primary human MC (hMC) cultures. METHODS: Dogs were anesthetized with IV propofol and received intradermal (ID) injections (50μL)...
October 27, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29103452/rational-approach-to-allergy-testing
#8
REVIEW
Michael P Platt, Jacqueline A Wulu
Allergy testing is commonly used when symptoms of allergic rhinitis are refractory to symptoms and there is potential for treatment with institution of avoidance measures or immunotherapy. Once the decision for testing has been made, the method of testing by either in vivo skin testing by prick/puncture or intradermal testing or in vitro testing of serum-specific IgE is dictated by factors in the clinical history and an informed decision by the patient. Because there is no perfect testing method, understanding the benefits and limitations of each method is important in selecting the best testing option for each patient...
December 2017: Otolaryngologic Clinics of North America
https://www.readbyqxmd.com/read/29100867/the-combined-utility-of-ex%C3%A2-vivo-ifn-%C3%AE-release-enzyme-linked-immunospot-assay-and-in%C3%A2-vivo-skin%C3%A2-testing-in-patients-with-antibiotic-associated-severe-cutaneous-adverse-reactions
#9
Jason A Trubiano, Kaija Strautins, Alec J Redwood, Rebecca Pavlos, Katherine C Konvinse, Ar Kar Aung, Monica A Slavin, Karin A Thursky, M Lindsay Grayson, Elizabeth J Phillips
BACKGROUND: For severe cutaneous adverse reactions (SCARs) associated with multiple antibiotics dosed concurrently, clinical causality is challenging and diagnostic approaches are limited, leading to constricted future antibiotic choices. OBJECTIVE: To examine the combined utility of in vivo and ex vivo diagnostic approaches at assigning drug causality in a cohort of patients with antibiotic-associated (AA)-SCARs. METHODS: Patients with AA-SCARs were prospectively recruited between April 2015 and February 2017...
October 31, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29076438/stevens-johnson-syndrome-versus-jarisch-herxheimer-reaction-in-an-hiv-positive-patient-with-secondary-syphilis
#10
Sarah Micozzi, Patricia Rojas Perez-Ezquerra, Cristina Morales-Cabeza, Cristina Diez, Manuel De Barrio
BACKGROUND: HIV positive patients can suffer many complications due to infectious diseases. A severe reaction to some of the drugs involved in the treatment can overlap with the symptoms of infections, making diagnosis very difficult. We present the case of an HIV positive 28-year-old-man, , with secondary syphilis, who developed a Stevens Johnson Syndrome (SJS) caused by one of the many drugs he was receiving. The SJS overlapped with a possible Jarisch Herxheimer Reaction, which complicated diagnosis of the skin reaction...
October 26, 2017: Recent Patents on Inflammation & Allergy Drug Discovery
https://www.readbyqxmd.com/read/29047169/non-immediate-reading-skin-tests-and-prolonged-challenges-in-non-immediate-hypersensitivity-to-beta-lactams-in-children
#11
G Lezmi, F Alrowaishdi, A Bados-Albiero, P Scheinmann, J de Blic, C Ponvert
BACKGROUND: A minority of children reporting non-immediate reactions to beta-lactams (BLs) are allergic. Allergy workup usually includes late-reading (48-72h) skin tests (ST) and short (1-3 days) drug provocation tests (DPT), regardless of the chronology of the index reaction. The sensitivity of hyper-late-reading (≥ 6-7 days) ST and of prolonged DPT for the diagnosis of non-immediate hypersensitiviy to BLs are yet to be determined. OBJECTIVES: To establish the diagnostic values of late- and hyper-late-reading ST and of prolonged DPT in children reporting non-immediate reactions to BLs...
October 19, 2017: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/29031391/a-dose-response-study-of-the-effect-of-prostaglandin-e2-on-thermal-nociceptive-sensitivity
#12
Anthony F Domenichiello, Breanne C Wilhite, Gregory S Keyes, Christopher E Ramsden
Inhibition of prostaglandin (PG) biosynthesis has been used to relieve pain for thousands of years. Today non-steroidal anti-inflammatory drugs (which largely inhibit PG synthesis) are widely used to treat pain. Four main types of PGs (PGD2, PGE2, PGF2 and PGI2) are synthesized from arachidonic acid during inflammation and have been demonstrated to impact nociception. PGE2 has been the most studied and utilized for its pain producing properties and has been demonstrated to increase hypersensitivity in rodent nociceptive behavioral models when applied centrally and/or peripherally...
November 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/29027520/further-evidence-associating-igg1-but-not-igg2-with-susceptibility-to-canine-visceral-leishmaniasis-caused-by-leishmania-l-infantum-chagasi-infection
#13
Luciana Vieira do R Lima, Liliane Almeida Carneiro, Marliane Batista Campos, Thiago Vasconcelos Dos Santos, Patrícia Karla Ramos, Márcia Dalastra Laurenti, Claudio Eduardo C Teixeira, Fernando T Silveira
We present here a cross-sectional study analyzing the IgG1 and IgG2 immune responses to natural canine Leishmania (L.) infantum chagasi-infection and their relationships with delayed-type hypersensitivity (DTH) in 50 mongrel dogs with previous positive serodiagnoses (IFAT-IgG) (56% with subclinical status [= apparently healthy] and 44% clinically sick), living in endemic areas for visceral leishmaniasis in the Brazilian Amazon. IgG1 and IgG2 responses were measured using commercial polyclonal antibodies in ELISA, while DTH was elicited by intradermal skin test using cultured promastigotes L...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/29023179/effect-of-platelet-rich-plasma-on-patients-after-blepharoplasty-surgery
#14
Fidelina Parra, David Enrique Morales-Rome, Rafael Campos-Rodríguez, Teresita Rocío Cruz-Hernández, Maria Elisa Drago-Serrano
AIM: To evaluate the effect of platelet-rich plasma (PRP) treatment on patients after blepharoplasty surgery. MATERIALS AND METHODS: After undergoing blepharoplasty, 20 patients were randomly divided into two groups (n = 10 each). One was treated with autologous PRP and the other was not given any post-surgery treatment (basal group). Autologous PRP application was performed intradermically 24 h, 1 month, and 2 months post-surgery, and the outcome of the applications was assessed 1, 2, and 3 months post-surgery...
October 12, 2017: Orbit
https://www.readbyqxmd.com/read/28982032/anti-inflammatory-effect-of-an-adhesive-resin-containing-indomethacin-loaded-nanocapsules
#15
Bruna Genari, Maria Beatriz Cardoso Ferreira, Liciane Fernandes Medeiros, Joice Soares de Freitas, Stefania Giotti Cioato, Iraci Lucena da Silva Torres, Adriana Raffin Pohlmann, Silvia Stanisçuaski Guterres, Vicente Castelo Branco Leitune, Fabri Cio Mezzomo Collares, Susana Maria Werner Samuel
OBJECTIVE: To analyze the anti-inflammatory and analgesic effects of an adhesive resin containing indomethacin-loaded nanocapsules in rat model. DESIGN: Adhesive resin disks with or without indomethacin-loaded nanocapsules were subcutaneously implanted into right hind paw of rats. A week after surgical procedure, 2% formalin solution was intradermally injected into plantar surface of paw. Nociceptive and inflammatory responses were evaluated by formalin test. Paw edema by pletismometer and mechanical hyperalgesia by von Frey test were performed on day 2, day 4, day 6, day 8, day 10 and day 12 after surgery...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28981507/reappraisal-of-leishmanin-skin-test-lst-in-the-management-of-american-cutaneous-leishmaniasis-a-retrospective-analysis-from-a-reference-center-in-argentina
#16
Alejandro Javier Krolewiecki, Maria Cristina Almazan, Marcelo Quipildor, Marisa Juarez, Jose Fernando Gil, Marco Espinosa, Maria Canabire, Silvana Pamela Cajal
Leishmania (Viannia) braziliensis is the species most frequently implicated with cutaneous and mucosal leishmaniasis in the Americas; its diagnosis is based on the identification of amastigotes in lesions, which is limited by low parasite burden. Leishmanin Skin Test (LST) is a support tool for diagnosis, based on delayed type hypersensitivity responses to Leishmania antigens injected intradermally, used in endemic areas as a complement to diagnosis. A retrospective analysis of individuals evaluated for their first episode of tegumentary leishmaniasis at a reference center in Argentina during the period 2006-2015 was performed, with the goal of assessing its usefulness as a support tool in the diagnosis of leishmaniasis...
October 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28973018/the-importance-of-communication-in-promoting-voluntary-participation-in-an-experimental-trial-a-qualitative-study-based-on-the-assessment-of-the-gamma-interferon-test-for-the-diagnosis-of-bovine-tuberculosis-in-france
#17
Clémence Boireau, Barbara Dufour, Anne Praud
Understanding the factors leading each stakeholder to participate in an experimental trial is a key element for improving trial set-up and for identifying selection bias in statistical analyses. An experimental protocol, validated by the European Commission, was developed in France to assess the ability of the gamma-interferon test in terms of accuracy to replace the second intradermal skin test in cases of suspected bovine tuberculosis. Implemented between 2013 and 2015, this experimental trial was based on voluntary participation...
2017: PloS One
https://www.readbyqxmd.com/read/28892515/strong-interferon-gamma-mediated-cellular-immunity-to-scrub-typhus-demonstrated-using-a-novel-whole-cell-antigen-elispot-assay-in-rhesus-macaques-and-humans
#18
Manutsanun Sumonwiriya, Daniel H Paris, Piyanate Sunyakumthorn, Tippawan Anantatat, Kemajittra Jenjaroen, Suchintana Chumseng, Rawiwan Im-Erbsin, Ampai Tanganuchitcharnchai, Suthatip Jintaworn, Stuart D Blacksell, Fazle R Chowdhury, Barbara Kronsteiner, Prapit Teparrukkul, Robin L Burke, Eric D Lombardini, Allen L Richards, Carl J Mason, James W Jones, Nicholas P J Day, Susanna J Dunachie
Scrub typhus is a febrile infection caused by the obligate intracellular bacterium Orientia tsutsugamushi, which causes significant morbidity and mortality across the Asia-Pacific region. The control of this vector-borne disease is challenging due to humans being dead-end hosts, vertical maintenance of the pathogen in the vector itself, and a potentially large rodent reservoir of unclear significance, coupled with a lack of accurate diagnostic tests. Development of an effective vaccine is highly desirable. This however requires better characterization of the natural immune response of this neglected but important disease...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28892222/oral-challenge-without-skin-tests-in-children-with-non-severe-betalactam-hypersensitivity-time-to-change-the-paradigm
#19
Luis Moral, Jean-Cristoph Caubet
Suspected allergy to penicillins and cephalosporins is very common in childhood. After a proper evaluation, allergy will be confirmed only in a small portion of them. Intradermal tests are usually part of the allergy workup, but they are painful for children and time-consuming, and their role has been debated. A systematic review found only two studies reporting a positive predictive value of skin tests in children of 36% and 33%, respectively, leading to a high rate of inaccurate diagnosis. Moreover, considering that skin tests are negative in more than 90-95% of cases, an oral provocation test (OPT) is finally needed to confirm tolerance in most of these children...
September 11, 2017: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/28864866/autoimmune-progesterone-dermatitis
#20
A Detrixhe, A F Nikkels, B Dezfoulian
Autoimmune progesterone dermatitis (APD) is an exceptional condition affecting young women of childbearing age with a high prevalence during the third decade of life. The diagnosis should be confirmed using an intradermal skin test to progesterone, during the follicular phase of the menstrual cycle. APD represents an early manifestation of autoimmune disease. A case of APD is presented who after curative treatment did not develop other autoimmune diseases during a 6-year follow-up. Dermatologists, gynecologists and obstetricians should be aware of this rare but highly invalidating entity...
November 2017: Archives of Gynecology and Obstetrics
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