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Miho Kono, Takeo Fujii, Genevieve Ray Lyons, Lei Huo, Roland Bassett, Yun Gong, Meghan Sri Karuturi, Debu Tripathy, Naoto T Ueno
PURPOSE: The purpose of this study is to evaluate survival outcome in patients with hormone receptor (HR)-positive (+) metastatic breast cancer (MBC) who received fluoxymesterone after disease progression while receiving contemporary hormonal therapy, as well as the association between estrogen receptor (ER)/androgen receptor (AR) status and survival outcome in these patients. METHODS: We retrospectively identified 103 patients treated with fluoxymesterone for HR + MBC from 2000 to 2014 and with at least one previous hormonal therapy in a metastatic setting...
November 2016: Breast Cancer Research and Treatment
Quentin Dumont, Mariana Bárcenas, Héloïse Dossmann, Isabelle Bailloux, Corinne Buisson, Nathalie Mechin, Adeline Molina, Françoise Lasne, Nalaka S Rannulu, Richard B Cole
Nonpolar anabolic steroids are doping agents that typically do not provide strong signals by electrospray ionization-mass spectrometry (ESI-MS) owing especially to the low polarity of the functional groups present. We have investigated the addition of anions, in ammonium salt form, to anabolic steroid samples as ionization enhancers and have confirmed that lower instrumental limits of detection (as low as 10 ng/mL for fluoxymesterone-M) are obtained by fluoride anion attachment mass spectrometry, as compared to ESI(+)/(-) or atmospheric pressure photoionization (APPI)(+)...
April 5, 2016: Analytical Chemistry
Tom Edward Ngo Lo, Zillien C Andal, Frances Lina Lantion-Ang
Gynaecomastia is a benign condition characterised by enlargement of the male breast. Drug-induced gynaecomastia merits deep consideration as it may account for as many as 25% of all cases of gynaecomastia in adults. Although the mechanism is not fully clear, some mechanisms include oestrogen-like activities, stimulation of testicular production of oestrogens, inhibition of testosterone synthesis or blockade of androgen action. Anabolic steroids, in particular when used during the pubertal stage, may cause significant irreversible gynaecomastia...
May 6, 2015: BMJ Case Reports
Vishal Saggar, Shenhong Wu, Maura N Dickler, Mario E Lacouture
BACKGROUND: Whereas the frequency of alopecia to cytotoxic chemotherapies has been well described, the incidence of alopecia during endocrine therapies (i.e., anti-estrogens, aromatase inhibitors) has not been investigated. Endocrine agents are widely used in the treatment and prevention of many solid tumors, principally those of the breast and prostate. Adherence to these therapies is suboptimal, in part because of toxicities. We performed a systematic analysis of the literature to ascertain the incidence and risk for alopecia in patients receiving endocrine therapies...
2013: Oncologist
Shanshan Yao, Yonggang Zhao, Xiaoping Li, Xiaohong Chen, Micong Jin
A method was developed for the determination of 11 anabolic hormones (boldenone, androstenedione, nandrolone, methandrostenolone, methyltestosterone, testosterone, testosterone acetate, trenbolone, testosterone propionate, stanozolol, fluoxymesterone) in fish by multi-function impurity adsorption solid-phase extraction-ultrafast liquid chromatography-tandem mass spectrometry. After the sample was extracted by methanol, the extract was cleaned-up quickly by C18 adsorbent, neutral alumina adsorbent and amino-functionalized nano-adsorbent...
June 2012: Se Pu, Chinese Journal of Chromatography
Jianghai Lu, Genye He, Xiaobing Wang, Youxuan Xu, Yun Wu, Ying Dong, Xin Liu, Zhenwen He, Jian Zhao, Hong Yuan
In this study fluoxymesterone urinary profiles were investigated by liquid chromatography quadrupole time-of-flight tandem mass spectrometry (LC-QTOFMS) with accurate mass measurement. Twelve metabolites including the parent drug were detected in two fluoxymesterone positive control urine samples. Three parameters were employed for evaluation of the accuracy of the chemical formulae in positive full scan experiment, which contained error between actual and calculated mass weights of prontonated and isotopic molecules together with abundance match between prontonated and isotopic molecules...
July 2012: Steroids
Cornelia Fürstenberger, Anna Vuorinen, Thierry Da Cunha, Denise V Kratschmar, Martial Saugy, Daniela Schuster, Alex Odermatt
Anabolic androgenic steroids (AAS) are testosterone derivatives used either clinically, in elite sports, or for body shaping with the goal to increase muscle size and strength. Clinically developed compounds and nonclinically tested designer steroids often marketed as food supplements are widely used. Despite the considerable evidence for various adverse effects of AAS use, the underlying molecular mechanisms are insufficiently understood. Here, we investigated whether some AAS, as a result of a lack of target selectivity, might inhibit 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2)-dependent inactivation of glucocorticoids...
April 2012: Toxicological Sciences: An Official Journal of the Society of Toxicology
Toktam Zohoorian-Abootorabi, Hamideh Sanee, Hediyeh Iranfar, Mohammad Reza Saberi, Jamshidkhan Chamani
This study was designed to examine the interaction of two anti-breast cancer drugs, i.e., fluoxymesterone (FLU) and cyclophosphamide (CYC), with human serum albumin (HSA) using different kinds of spectroscopic, zeta potential and molecular modeling techniques under imitated physiological conditions. The RLS technique was utilized to investigate the effect of the two anticancer drugs on changes of the protein conformation, both separately and simultaneously. Our study suggested that the enhancement in RLS intensity was attributed to the formation of a new complex between the two drugs and the protein...
March 2012: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
E M Malone, C T Elliott, D G Kennedy, L Regan
A rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the simultaneous identification, confirmation and quantitation of thirteen synthetic growth promoters in bovine muscle. The method was validated in accordance with the criteria defined in Commission Decision 2002/657/EC. A value of 1mugkg(-1) was chosen as the required performance level (RPL) for all analytes. The growth promoters investigated were alpha and beta trenbolone, 16-beta-OH stanozolol, methylboldenone, fluoxymesterone, methyltestosterone, medroxyprogesterone acetate, megestrol acetate, melengestrol acetate, dexamethasone, flumethasone, dienestrol and hexestrol...
April 1, 2009: Analytica Chimica Acta
Masayuki Yamada, Sugako Aramaki, Masahiko Kurosawa, Isao Kijima-Suda, Koichi Saito, Hiroyuki Nakazawa
The use of anabolic steroids in racehorses is strictly regulated. We have developed a method for the simultaneous analysis of 11 anabolic steroids: fluoxymesterone, 17alpha-methyltestosterone, mestanolone, methandienone, methandriol, oxymetholone, boldenone, furazabol, methenolone, nandrolone, and stanozolol, for possible application to a doping test in racehorses. We selected 15 kinds of target substances for a doping test from the main metabolites of these anabolic steroids, and established a method for simultaneous analysis...
September 2008: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Masayuki Yamada, Sugako Aramaki, Tomoo Hosoe, Masahiko Kurosawa, Isao Kijima-Suda, Koichi Saito, Hiroyuki Nakazawa
Fluoxymesterone, an anabolic steroid with the 17alpha-methyl,17beta-hydroxy group, has been developed as an oral formulation for therapeutic purposes. However, it is also used illegally in racehorses to enhance racing performance. In this study, we detected 9alpha-fluoro-17,17-dimethyl-18-norandrostane-4,13-dien-11beta-ol-3-one by gas chromatography/mass spectrometry (GC/MS), which has not been reported as a fluoxymesterone metabolite so far in horse. It was synthesized for use as a reference standard, and characterized on the basis of (1)H NMR and (13)C NMR spectra, as well as GC/MS EI mass spectra of TMS derivatives...
July 2008: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
R Muñiz-Valencia, R Gonzalo-Lumbreras, A Santos-Montes, R Izquierdo-Hornillos
An isocratic HPLC method for the determination with screening purposes of anabolic androgenic steroids (AASs: fluoxymesterone, boldenone, nortestosterone, metandrostenolone, norethindrone, methyltestosterone and bolasterone), used as growth promoting agents, in finishing pig feed samples has been developed and validated. The separation was achieved by using a reversed-phase Chromolith RP-18e column at controlled temperature, UV-detection at 245nm and epitestosterone as internal standard. The method development involved optimization of different aqueous-organic mobile phases using methanol or acetonitrile as organic modifiers, flow-rate and temperature...
March 17, 2008: Analytica Chimica Acta
Oscar J Pozo, Koen Deventer, Peter Van Eenoo, Frans T Delbeke
The detection of new anabolic steroid metabolites and new designer steroids in urine is a challenge in doping analysis. An approach based on precursor ion scanning for the detection of unknown anabolic steroids and metabolites is proposed. The study of the MS/MS spectra of selected anabolic steroids revealed different fragmentation pathways at low and medium collision energy depending on the steroid structure. However, after analysis at high collision energy three common ions at m/z 105, m/z 91, and m/z 77 were found for all studied anabolic steroids...
March 1, 2008: Analytical Chemistry
Oscar J Pozo, Wim Van Thuyne, Koen Deventer, Peter Van Eenoo, Frans T Delbeke
The suitability of liquid chromatography tandem mass spectrometry (LC-MS/MS) and gas chromatography mass spectrometry (GC-MS) for the elucidation of fluoxymesterone metabolism has been evaluated. Electrospray ionization (ESI) and collision induced dissociation (CID) fragmentation in LC-MS/MS and electron impact spectra (EI) in GC-MS have been studied for fluoxymesterone and two commercially available metabolites. MS(n) experiments and accurate mass measurements performed by an ion-trap analyser and a QTOF instrument respectively have been used for the elucidation of the fragmentation pathway...
March 2008: Journal of Mass Spectrometry: JMS
Lotta K Amundsen, Heli Sirén
ACE is a popular technique for evaluating association constants between drugs and proteins. However, ACE has not previously been applied to study the association between electrically neutral biomolecules and plasma proteins. We studied the affinity between human and bovine serum albumins (HSA and BSA, respectively) and three neutral endogenous steroid hormones (testosterone, epitestosterone and androstenedione) and two synthetic analogues (methyltestosterone and fluoxymesterone) by applying the partial-filling technique in ACE (PF-ACE)...
October 2007: Electrophoresis
R Gonzalo-Lumbreras, R Muñiz-Valencia, A Santos-Montes, R Izquierdo-Hornillos
A LC isocratic separation study of a complex mixture containing 18 steroids (corticoids and anabolics), used potentially as growth promoters, was carried out. For this purpose, using a Hypersil ODS column at controlled temperature, mobile phases (from binary to quaternary) prepared from water and MeOH, ACN or THF as organic modifiers and UV detection at 245 nm, were employed (dehydroepiandrosterone was detected at 200 nm). The optimum separation was achieved using water/acetonitrile (65:35, v/v) as mobile phase at 30 degrees C, allowing the separation of 16 out of 18 steroids in about 30 min...
July 13, 2007: Journal of Chromatography. A
Alain Berrebi, Edit Feldberg, Irena Spivak, Lev Shvidel
We report a patient with very advanced myelofibrosis and huge splenomegaly who showed a complete hematological response to low dose thalidomide with reversal of splenomegaly and bone narrow fibrosis after 30 months of the treatment.
February 2007: Haematologica
Jeffrey N Miner, William Chang, Mark S Chapman, Patricia D Finn, Mei Hua Hong, Francisco J López, Keith B Marschke, Jon Rosen, William Schrader, Russell Turner, Arjan van Oeveren, Humberto Viveros, Lin Zhi, Andres Negro-Vilar
A number of conditions, including osteoporosis, frailty, and sexual dysfunction in both men and women have been improved using androgens. However, androgens are not widely used for these indications because of the side effects associated with these drugs. We describe an androgen receptor (AR) ligand that maintains expected anabolic activities with substantially diminished activity in the prostate. LGD2226 is a nonsteroidal, nonaromatizable, highly selective ligand for the AR, exhibiting virtually no affinity for the other intracellular receptors...
January 2007: Endocrinology
Lotta K Amundsen, Heli Sirén
Separation of anabolic and androgenic steroids by micellar electrokinetic chromatography (MEKC) has been little studied. Simultaneous separation of the endogenous alpha-epimers testosterone and epitestosterone has not been achieved with any electroseparation technique. Here, a partial filling micellar electrokinetic chromatographic (PF-MEKC) method is described for the analysis of three endogenous steroid hormones (androstenedione, testosterone, epitestosterone) and two synthetic anabolic steroids (fluoxymesterone, methyltestosterone)...
October 27, 2006: Journal of Chromatography. A
Shingo Takada, Yasushi Miyagawa, Akihiko Okuyama
No abstract text is available yet for this article.
June 28, 2006: Nihon Rinsho. Japanese Journal of Clinical Medicine
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