keyword
MENU ▼
Read by QxMD icon Read
search

ARP2

keyword
https://www.readbyqxmd.com/read/27904039/role-of-rac1-wave2-signaling-in-mediating-the-inhibitory-effects-of-%C3%AE-tocotrienol-on-mammary-cancer-cell-migration-and-invasion
#1
Ibrahim Gayadh Algayadh, Venkateshwararao Dronamraju, Paul William Sylvester
The majority of breast cancer deaths result from the progression of this disease to a metastatic phenotype. Rac1 and Cdc42 are Rho family members that together with their downstream effectors, Wiskott-Aldrich Syndrome protein-family verprolin-homologous protein 2 (WAVE2) and Arp2/3, play an important role in cytoskeletal reorganization and the formation of membrane protrusions that promote cancer cell migration and invasion. γ-Tocotrienol, is a natural isoform within the vitamin E family of compounds that inhibits breast cancer cell growth and progression by suppressing various signaling pathways involved in mitogenic signaling and metastatic progression...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27900558/the-role-of-viral-protein-ac34-in-nuclear-relocation-of-subunits-of-the-actin-related-protein-2-3-complex
#2
Jingfang Mu, Yongli Zhang, Yangyang Hu, Xue Hu, Yuan Zhou, Xinwen Chen, Yun Wang
The actin nucleator actin-related protein complex (Arp2/3) is composed of seven subunits: Arp2, Arp3, p40/ARPC1 (P40), p34/ARPC2 (P34), p21/ARPC3 (P21), p20/ARPC4 (P20), and p16/ARPC5 (P16). Arp2/3 plays crucial roles in a variety of cellular activities through regulation of actin polymerization. Autographa californica multiple nucleopolyhedrovirus (AcMNPV), one of the best-studied alphabaculoviruses, induces Arp2/3 nuclear relocation and mediates nuclear actin polymerization to assist in virus replication...
December 5, 2016: Virologica Sinica
https://www.readbyqxmd.com/read/27884932/drosophila-wash-is-required-for-integrin-mediated-cell-adhesion-cell-motility-and-lysosomal-neutralization
#3
Benedikt M Nagel, Meike Bechtold, Luis Garcia Rodriguez, Sven Bogdan
The Wiskott-Aldrich Syndrome Protein and SCAR Homologue (WASH) is a conserved actin nucleation promoting factor controlling Arp2/3 complex activity in endosomal sorting and recycling. Previous studies have identified WASH as an essential regulator in Drosophila development. Here, we show that homozygous wash mutant flies are viable and fertile. We demonstrate that Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. WASH generates actin patches on endosomes and lysosomes mediating both functions...
November 24, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27872092/plk4-promotes-cancer-invasion-and-metastasis-through-arp2-3-complex-regulation-of-the-actin-cytoskeleton
#4
Karineh Kazazian, Christopher D Go, Hannah Wu, Olga Brashavitskaya, Roland S Z Xu, James W Dennis, Anne-Claude Gingras, Carol Swallow
The polo family serine threonine kinase Plk4 has been proposed as a therapeutic target in advanced cancers based on increased expression in primary human cancers, facilitation of tumor growth in murine xenograft models, and centrosomal amplification induced by its overexpression. However, both the causal link between these phenomena and the feasibility of selective Plk4 inhibition remain unclear. Here we characterize Plk4-dependent cancer cell migration and invasion as well as local invasion and metastasis of cancer xenografts...
November 21, 2016: Cancer Research
https://www.readbyqxmd.com/read/27870871/functional-genomic-analysis-of-candida-albicans-adherence-reveals-a-key-role-for-the-arp2-3-complex-in-cell-wall-remodelling-and-biofilm-formation
#5
Jason A Lee, Nicole Robbins, Jinglin L Xie, Troy Ketela, Leah E Cowen
Fungal biofilms are complex, structured communities that can form on surfaces such as catheters and other indwelling medical devices. Biofilms are of particular concern with Candida albicans, one of the leading opportunistic fungal pathogens of humans. C. albicans biofilms include yeast and filamentous cells that are surrounded by an extracellular matrix, and they are intrinsically resistant to antifungal drugs such that resolving biofilm infections often requires surgery to remove the contaminated device. C...
November 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27864076/electric-field-as-a-potential-directional-cue-in-homing-of-bone-marrow-derived-mesenchymal-stem-cells-to-cutaneous-wounds
#6
Eliza Zimolag, Julia Borowczyk-Michalowska, Sylwia Kedracka-Krok, Bozena Skupien-Rabian, Elzbieta Karnas, Slawomir Lasota, Jolanta Sroka, Justyna Drukala, Zbigniew Madeja
Bone marrow-derived cells are thought to participate and enhance the healing process contributing to skin cells or releasing regulatory cytokines. Directional cell migration in a weak direct current electric field (DC-EF), known as electrotaxis, may be a way of cell recruitment to the wound site. Here we examined the influence of electric field on bone marrow adherent cells (BMACs) and its potential role as a factor attracting mesenchymal stem cells to cutaneous wounds. We observed that in an external EF, BMAC movement was accelerated and highly directed with distinction of two cell populations migrating toward opposite poles: mesenchymal stem cells migrated toward the cathode, whereas macrophages toward the anode...
November 15, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27862269/arp2-3-complex-and-mps3-are-required-for-regulation-of-ribosome-biosynthesis-in-the-secretory-stress-response
#7
Yukari Yabuki, Masako Katayama, Yushi Kodama, Akiko Sakamoto, Ayumi Yatsuhashi, Kouichi Funato, Keiko Mizuta
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae. However, the molecular mechanism underlying secretory defect-induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1-228 and cmd1-239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, fail to interact with Arc35, a component of the Arp2/3 complex...
November 10, 2016: Yeast
https://www.readbyqxmd.com/read/27852899/local-arp2-3-dependent-actin-assembly-modulates-applied-traction-force-during-apcam-adhesion-site-maturation
#8
Kenneth B Buck, Andrew W Schaefer, Vincent T Schoonderwoert, Matthew S Creamer, Eric R Dufresne, Paul Forscher
Homophilic binding of Ig superfamily molecules, such as apCAM, leads to actin filament assembly near nascent adhesion sites. Such actin assembly can generate significant localized forces that have not been characterized in the larger context of axon growth and guidance. We used apCAM coated bead substrates applied to the surface of neuronal growth cones to characterize the development of forces evoked by varying stiffness of mechanical restraint. Unrestrained bead propulsion matched or exceeded rates of retrograde network flow and was dependent on Arp2/3 complex activity...
November 16, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27821733/a-diaphanous-related-formin-links-ras-signaling-directly-to-actin-assembly-in-macropinocytosis-and-phagocytosis
#9
Alexander Junemann, Vedrana Filić, Moritz Winterhoff, Benjamin Nordholz, Christof Litschko, Helena Schwellenbach, Till Stephan, Igor Weber, Jan Faix
Phagocytosis and macropinocytosis are Ras-regulated and actin-driven processes that depend on the dynamic rearrangements of the plasma membrane that protrudes and internalizes extracellular material by cup-shaped structures. However, the regulatory mechanisms underlying actin assembly in large-scale endocytosis remain elusive. Here, we show that the Diaphanous-related formin G (ForG) from the professional phagocyte Dictyostelium discoideum localizes to endocytic cups. Biochemical analyses revealed that ForG is a rather weak nucleator but efficiently elongates actin filaments in the presence of profilin...
November 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27805253/cortactin-contributes-to-the-tumorigenicity-of-colorectal-cancer-by-promoting-cell-proliferation
#10
Huo Wu, Xi Cheng, Xiaopin Ji, Yonggang He, Xiaoqian Jing, Haoxuan Wu, Ren Zhao
Cortactin is a scaffolding protein that regulates Arp2/3-mediated actin polymerization. We showed in a previous study that cortactin was highly expressed in human stage II-III colorectal cancer (CRC) tissues. In the present study, using colony formation and CCK-8 assays, we showed that overexpression of cortactin accelerated the proliferation of CRC cells. Flow cytometric assays revealed that cortactin promoted G1/S phase cell cycle transition. Later, we constructed the phosphorylation mutation of cortactin at the Tyr421 residue...
October 27, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27802336/autographa-californica-multiple-nucleopolyhedrovirus-ac34-protein-retains-cellular-actin-related-protein-2-3-complex-in-the-nucleus-by-subversion-of-crm1-dependent-nuclear-export
#11
Jingfang Mu, Yongli Zhang, Yangyang Hu, Xue Hu, Yuan Zhou, He Zhao, Rongjuan Pei, Chunchen Wu, Jizheng Chen, Han Zhao, Kai Yang, Monique M van Oers, Xinwen Chen, Yun Wang
Actin, nucleation-promoting factors (NPFs), and the actin-related protein 2/3 complex (Arp2/3) are key elements of the cellular actin polymerization machinery. With nuclear actin polymerization implicated in ever-expanding biological processes and the discovery of the nuclear import mechanisms of actin and NPFs, determining Arp2/3 nucleo-cytoplasmic shuttling mechanism is important for understanding the function of nuclear actin. A unique feature of alphabaculovirus infection of insect cells is the robust nuclear accumulation of Arp2/3, which induces actin polymerization in the nucleus to assist in virus replication...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27799897/enhancement-of-peripheral-nerve-regrowth-by-the-purine-nucleoside-analog-and-cell-cycle-inhibitor-roscovitine
#12
Vincent Law, Sophie Dong, Jesusa L Rosales, Myung-Yung Jeong, Douglas Zochodne, Ki-Young Lee
Peripheral nerve regeneration is a slow process that can be associated with limited outcomes and thus a search for novel and effective therapy for peripheral nerve injury and disease is crucial. Here, we found that roscovitine, a synthetic purine nucleoside analog, enhances neurite outgrowth in neuronal-like PC12 cells. Furthermore, ex vivo analysis of pre-injured adult rat dorsal root ganglion (DRG) neurons showed that roscovitine enhances neurite regrowth in these cells. Likewise, in vivo transected sciatic nerves in rats locally perfused with roscovitine had augmented repopulation of new myelinated axons beyond the transection zone...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27799367/formin-generated-actomyosin-arcs-propel-t-cell-receptor-microcluster-movement-at-the-immune-synapse
#13
Sricharan Murugesan, Jinsung Hong, Jason Yi, Dong Li, Jordan R Beach, Lin Shao, John Meinhardt, Grey Madison, Xufeng Wu, Eric Betzig, John A Hammer
Actin assembly and inward flow in the plane of the immunological synapse (IS) drives the centralization of T cell receptor microclusters (TCR MCs) and the integrin leukocyte functional antigen 1 (LFA-1). Using structured-illumination microscopy (SIM), we show that actin arcs populating the medial, lamella-like region of the IS arise from linear actin filaments generated by one or more formins present at the IS distal edge. After traversing the outer, Arp2/3-generated, lamellipodia-like region of the IS, these linear filaments are organized by myosin II into antiparallel concentric arcs...
November 7, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27791462/deregulation-of-focal-adhesion-formation-and-cytoskeletal-tension-due-to-loss-of-a-type-lamins
#14
Tobias D J Corne, Tom Sieprath, Jonathan Vandenbussche, Danahe Mohammed, Mariska Te Lindert, Kris Gevaert, Sylvain Gabriele, Katarina Wolf, Winnok H De Vos
The nuclear lamina mechanically integrates the nucleus with the cytoskeleton and extracellular environment and regulates gene expression. These functions are exerted through direct and indirect interactions with the lamina's major constituent proteins, the A-type lamins, which are encoded by the LMNA gene. Using quantitative stable isotope labeling-based shotgun proteomics we have analyzed the proteome of human dermal fibroblasts in which we have depleted A-type lamins by means of a sustained siRNA-mediated LMNA knockdown...
October 28, 2016: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/27791032/v-1-regulates-capping-protein-activity-in-vivo
#15
Goeh Jung, Christopher J Alexander, Xufeng S Wu, Grzegorz Piszczek, Bi-Chang Chen, Eric Betzig, John A Hammer
Capping Protein (CP) plays a central role in the creation of the Arp2/3-generated branched actin networks comprising lamellipodia and pseudopodia by virtue of its ability to cap the actin filament barbed end, which promotes Arp2/3-dependent filament nucleation and optimal branching. The highly conserved protein V-1/Myotrophin binds CP tightly in vitro to render it incapable of binding the barbed end. Here we addressed the physiological significance of this CP antagonist in Dictyostelium, which expresses a V-1 homolog that we show is very similar biochemically to mouse V-1...
October 25, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27780040/functional-coordination-of-wave-and-wasp-in-c-%C3%A2-elegans-neuroblast-migration
#16
Zhiwen Zhu, Yongping Chai, Yuxiang Jiang, Wenjing Li, Huifang Hu, Wei Li, Jia-Wei Wu, Zhi-Xin Wang, Shanjin Huang, Guangshuo Ou
Directional cell migration is critical for metazoan development. We define two molecular pathways that activate the Arp2/3 complex during neuroblast migration in Caenorhabditis elegans. The transmembrane protein MIG-13/Lrp12 is linked to the Arp2/3 nucleation-promoting factors WAVE or WASP through direct interactions with ABL-1 or SEM-5/Grb2, respectively. WAVE mutations partially impaired F-actin organization and decelerated cell migration, and WASP mutations did not inhibit cell migration but enhanced migration defects in WAVE-deficient cells...
October 24, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27780037/wasp-and-wave-team-up-at-the-leading-edge
#17
Nicolas Molinie, Alexis Gautreau
Arp2/3-dependent branched actin networks drive membrane protrusions, with WAVE being recognized as the critical Arp2/3 activator in this process. In this issue of Developmental Cell, Zhu et al. (2016) demonstrate that WASP, an Arp2/3 activator mostly involved in endocytosis, collaborates with WAVE to promote migration of neuroblasts in Caenorhabditis elegans.
October 24, 2016: Developmental Cell
https://www.readbyqxmd.com/read/27778524/the-actin-binding-proteins-cortactin-and-hs1-are-dispensable-for-platelet-actin-nodule-and-megakaryocyte-podosome-formation
#18
Steven G Thomas, Natalie S Poulter, Danai Bem, Brenda Finney, Laura M Machesky, Stephen P Watson
A dynamic, properly organised actin cytoskeleton is critical for the production and haemostatic function of platelets. The Wiskott Aldrich Syndrome protein (WASp) and Actin-Related Proteins 2 & 3 Complex (Arp2/3 complex) are critical mediators of actin polymerisation and organisation in many cell types. In platelets and megakaryocytes, these proteins have been shown to be important for proper platelet production and function. The cortactin family of proteins (Cttn & HS1) are known to regulate WASp-Arp2/3-mediated actin polymerisation in other cell types and so here we address the role of these proteins in platelets using knockout mouse models...
October 25, 2016: Platelets
https://www.readbyqxmd.com/read/27775702/diversified-actin-protrusions-promote-environmental-exploration-but-are-dispensable-for-locomotion-of%C3%A2-leukocytes
#19
Alexander Leithner, Alexander Eichner, Jan Müller, Anne Reversat, Markus Brown, Jan Schwarz, Jack Merrin, David J J de Gorter, Florian Schur, Jonathan Bayerl, Ingrid de Vries, Stefan Wieser, Robert Hauschild, Frank P L Lai, Markus Moser, Dontscho Kerjaschki, Klemens Rottner, J Victor Small, Theresia E B Stradal, Michael Sixt
Most migrating cells extrude their front by the force of actin polymerization. Polymerization requires an initial nucleation step, which is mediated by factors establishing either parallel filaments in the case of filopodia or branched filaments that form the branched lamellipodial network. Branches are considered essential for regular cell motility and are initiated by the Arp2/3 complex, which in turn is activated by nucleation-promoting factors of the WASP and WAVE families. Here we employed rapid amoeboid crawling leukocytes and found that deletion of the WAVE complex eliminated actin branching and thus lamellipodia formation...
October 24, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27771248/dynamin2-gtpase-contributes-to-invadopodia-formation-in-invasive-bladder-cancer-cells
#20
Yubai Zhang, Maya Nolan, Hiroshi Yamada, Masami Watanabe, Yasutomo Nasu, Kohji Takei, Tetsuya Takeda
Cancer cell invasion is mediated by actin-based membrane protrusions termed invadopodia. Invadopodia consist of "core" F-actin bundles associated with adhesive and proteolytic machineries promoting cell invasion by degrading extracellular matrix (ECM). Formation of the F-actin core in invadopodia is regulated by various actin-binding proteins including Arp2/3 complex and cortactin. Dynamin GTPase localizes to the invadopodia and is implicated in cancer cell invasion, but its precise role at the invadopodia remained elusive...
October 19, 2016: Biochemical and Biophysical Research Communications
keyword
keyword
2647
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"