keyword
MENU ▼
Read by QxMD icon Read
search

Xpert

keyword
https://www.readbyqxmd.com/read/28318702/antibiotic-associated-diarrhea-clinical-characteristics-and-the-presence-of-clostridium-difficile
#1
A Rodríguez-Varón, O M Muñoz, J Pulido-Arenas, S B Amado, M Tobón-Trujillo
INTRODUCTION AND AIMS: Evidence in Colombia and Latin America has been insufficient for establishing the clinical characteristics of patients with antibiotic-associated diarrhea (AAD). The present study attempts to describe the clinical characteristics of patients with AAD and to determine the presence of Clostridium difficile, utilizing the polymerase chain reaction (PCR) technique. MATERIALS AND METHODS: Forty-three patients with AAD, managed at the Hospital Universitario San Ignacio in Bogota, Colombia, were evaluated...
March 15, 2017: Revista de Gastroenterología de México
https://www.readbyqxmd.com/read/28317811/performance-of-the-xpert-mtb-rif-assay-in-the-diagnosis-of-tuberculosis-in-formalin-fixed-paraffin-embedded-tissues
#2
Pascal Polepole, Mwila Kabwe, Mpanga Kasonde, John Tembo, Aaron Shibemba, Justin O'Grady, Nathan Kapata, Alimuddin Zumla, Matthew Bates
OBJECTIVE/BACKGROUND: Extrapulmonary tuberculosis (EPTB), which accounts for 10%-40% of the global burden of TB, with the highest incidence in Sub-Saharan Africa, is strongly associated with human immunodeficiency virus infection. Diagnosing EPTB is challenging, and recently, there has been a concerted effort to evaluate the latest molecular diagnostics for diagnosing TB in a range of specimen types. The Xpert MTB/RIF assay (Cepheid, Sunnyvale, CA, USA) is one such technology, which simultaneously detects Mycobacterium tuberculosis and rifampicin resistance...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28317800/are-who-approved-nucleic-acid-amplification-tests-causing-large-scale-false-identification-of-rifampicin-resistant-tuberculosis-programmatic-experience-from-south-india
#3
Praveen Sanker, Anusree P Ambika, Vishnu T Santhosh, Ramya Puthukkudi Kottuthodi, Ravikrishnan Balakrishnan, Sunil Kumar Mrithunjayan, Hisham Moosan
INTRODUCTION: The nucleic acid amplification tests (NAATs): Line probe assay and GeneXpert (Xpert) have evolved as the primary tool for identification of rifampicin (RIF)-resistant (RR) tuberculosis (TB) worldwide, primarily because of the ease and speed. We rechecked RR isolates identified by NAATs from presumptive RR TB cases belonging to South India by the Revised National TB Control Program, India using multiple RIF concentrations on Bactec MGIT system and compared the mutation patterns with the resistance levels...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28285563/impact-of-introduction-of-xpert-flu-assay-for-influenza-pcr-testing-on-obstetric-patients-a-quality-improvement-project
#4
Eimear Linehan, Marian Brennan, Sadhbh O'Rourke, Suzie Coughlan, Lisa Clooney, David LeBlanc, Joanna Griffin, Maeve Eogan, Richard J Drew
OBJECTIVE: The aim of this project was to assess the implementation of a quality improvement project regarding the introduction of on-site influenza PCR testing in a stand-alone obstetric hospital. METHODS: As part of a quality improvement project on the management of influenza in obstetric patients, the Xpert Flu assay (Cepheid, USA) was introduced on-site and it replaced the previous method of PCR testing which was off-site. The main outcome measures were duration of antimicrobials, rate of admission and administration of oseltamavir in the emergency department...
March 12, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28284274/initial-screening-of-extra-pulmonary-tuberculosis-using-the-xpert-mtb-rif-assay-improves-case-detection-rates
#5
Amita Jain, Pravin Kumar Singh, Urmila Singh, Vijay Kumar
No abstract text is available yet for this article.
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28284253/effect-of-previous-treatment-and-sputum-quality-on-diagnostic-accuracy-of-xpert-%C3%A2-mtb-rif
#6
C Acuña-Villaorduña, P Orikiriza, D Nyehangane, L F White, J Mwanga-Amumpaire, S Kim, M Bonnet, K P Fennelly, Y Boum, E C Jones-López
SETTING: In early studies, Xpert® MTB/RIF accurately detected culture-proven pulmonary tuberculosis (TB). Recent reports have, however, found a lower than expected specificity in previously treated TB patients. OBJECTIVE: To investigate the diagnostic accuracy of Xpert in presumptive pulmonary TB patients in Southwestern Uganda. DESIGN: We obtained demographic and clinical information and collected three sputum samples from each patient for smear microscopy, Xpert and culture...
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28284252/operational-modelling-the-mechanisms-influencing-tb-diagnostic-yield-in-an-xpert-%C3%A2-mtb-rif-based-algorithm
#7
R Dunbar, P Naidoo, N Beyers, I Langley
SETTING: Cape Town, South Africa. OBJECTIVE: To compare the diagnostic yield for smear/culture and Xpert® MTB/RIF algorithms and to investigate the mechanisms influencing tuberculosis (TB) yield. METHOD: We developed and validated an operational model of the TB diagnostic process, first with the smear/culture algorithm and then with the Xpert algorithm. We modelled scenarios by varying TB prevalence, adherence to diagnostic algorithms and human immunodeficiency virus (HIV) status...
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28284251/bottom-up-or-top-down-unit-cost-estimation-of-tuberculosis-diagnostic-tests-in-india
#8
S Rupert, A Vassall, N Raizada, S D Khaparde, C Boehme, V S Salhotra, K S Sachdeva, S A Nair, A H Van't Hoog
SETTING: Of 18 sites that participated in an implementation study of the Xpert® MTB/RIF assay in India, we selected five microscopy centres and two reference laboratories. OBJECTIVE: To obtain unit costs of diagnostic tests for tuberculosis (TB) and drug-resistant TB. DESIGN: Laboratories were purposely selected to capture regional variations and different laboratory types. Both bottom-up and the top-down methods were used to estimate unit costs...
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28281441/miliary-tuberculosis
#9
Surendra K Sharma, Alladi Mohan
Miliary tuberculosis (TB) results from a massive lymphohematogenous dissemination of Mycobacterium tuberculosis bacilli and is characterized by tiny tubercles evident on gross pathology resembling millet seeds in size and appearance. The global HIV/AIDS pandemic and widespread use of immunosuppressive drugs and biologicals have altered the epidemiology of miliary TB. Considered to be predominantly a disease of infants and children in the pre-antibiotic era, miliary TB is increasingly being encountered in adults as well...
March 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28278193/a-bcr-abl1-cutoff-of-1-5-at-3-months-determined-by-the-genexpert-system-predicts-an-optimal-response-in-patients-with-chronic-myeloid-leukemia
#10
Valentín García-Gutiérrez, María T Gómez-Casares, José M Puerta, Juan M Alonso-Domínguez, Santiago Osorio, Juan C Hernández-Boluda, Rosa Collado, María J Ramírez, Fátima Ibáñez, María L Martín, Juan D Rodríguez-Gambarte, Carolina Martínez-Laperche, Montse Gómez, Dolly V Fiallo, Sara Redondo, Alicia Rodríguez, Concepción Ruiz-Nuño, Juan L Steegmann, Antonio Jiménez-Velasco
In chronic myeloid leukemia (CML) patients, 3-month BCR-ABL1 levels have consistently been correlated with further outcomes. Monitoring molecular responses in CML using the GeneXpert (Cepheid) platform has shown an optimal correlation with standardized RQ-PCR (IS) when measuring BCR-ABL1 levels lower than 10%, as it is not accurate for values over 10%. The aim of the present study was to determine the predictive molecular value at three months on different outcome variables using the Xpert BCR-ABL1 MonitorTM assay (Xpert BCR-ABL1)...
2017: PloS One
https://www.readbyqxmd.com/read/28275079/multicentre-evaluation-of-the-cepheid-xpert%C3%A2-hepatitis-c-virus-hcv-viral-load-assay
#11
M P McHugh, A H B Wu, S Chevaliez, J M Pawlotsky, M Hallin, K E Templeton
Viral load monitoring for hepatitis C virus (HCV) is necessary to diagnose infection and monitor response to therapy but the tests involved are currently confined to specialist institutions. There is need for a fast, accurate assay with limited operator input to enhance the access to viral load monitoring. We evaluated the quantification of HCV RNA in serum and plasma by the Cepheid Xpert® HCV Viral Load assay in comparison to the Abbott RealTime HCV assay. Serum and plasma samples from HCV infected individuals at four international sites were gathered...
March 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28275073/rapid-detection-of-cell-free-mycobacterium-tuberculosis-dna-in-tuberculous-pleural-effusion
#12
Nanying Che, Xinting Yang, Zichen Liu, Kun Li, Xiaoyou Chen
Tuberculous pleurisy is one of the most common extra-pulmonary tuberculosis, but its diagnosis remains to be difficult. In this study, we for the first time report detection of cell-free Mycobacterium tuberculosis DNA in pleural effusion and evaluation of this newly developed molecular assay. A total of 78 patients with pleural effusion, 60 patients with tuberculous pleurisy and 18 patients with alternative diseases, were included in this study. Mycobacterial culture, Xpert MTB/RIF assay, adenosine deaminase assay, T-SPOT...
March 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28274126/-selection-of-laboratory-procedures-to-detect-toxigenic-by-the-2-step-method
#13
Yoko Tanino, Mai Kodama, Hiroomi Daicho, Yoshito Miyauchi, Towa Yasumoto, Yukiji Yamada, Noriko Kyotani, Satoko Kurahashi, Masaji Ushiyama, Takeshi Kimura, Toshiaki Komori, Yumiko Fujitomo, Masaki Nakanishi, Naohisa Fujita
The 2-step method is an algorithm to detect toxigenic Clostridium difficile. We herein compared the sensitivities and specificities of an enzyme immunoassay (toxin A/B-EIA), toxigenic culture (TC-EIA), Loop-Mediated Isothermal Amplification assay (LAMP), and Xpert C. difficile (Xpert) with the detection of the toxin B gene by a polymerase chain reaction (PCR). The results obtained showed that the sensitivities and specificities of toxin A/B-EIA, Xpert, TC-EIA, and LAMP were 30 and 100%, 87.2 and 100%, 97.5 and 89...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28264670/examining-the-quality-of-childhood-tuberculosis-diagnosis-in-cambodia-a-cross-sectional-study
#14
Julia B Frieze, Rajendra-Prasad Yadav, Khann Sokhan, Song Ngak, Team Bak Khim
BACKGROUND: Cambodia is one of the 22 countries with the highest TB burden. While childhood TB is estimated to make up 10-20% of total TB cases in high-burden settings, this proportion ranges from 1.3 to 39.4% throughout Cambodia's provinces, suggesting potential under- and over-diagnosis of childhood TB, subnationally. The proportion of case notifications classified as extrapulmonary TB out of total TB case notifications in children is 87%, greatly exceeding the expected global range of 20-30%...
March 6, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28255603/intraoperative-diagnosis-of-staphylococcus-aureus-and-coagulase-negative-staphylococcus-using-xpert-mrsa-sa-ssti-assay-in-prosthetic-joint-infection
#15
Andrea Sambri, Giovanni Pignatti, Matteo Romagnoli, Davide Donati, Maurilio Marcacci, Matteo Cadossi
The aim of this prospective study was to evaluate the performance of the Polymerase Chain Reaction (PCR) tool Xpert MRSA/SA SSTI test (Cepheid, Sunnyvale, CA, USA) on periprosthetic samples from a cohort of patients with suspected prosthetic joint infection (PJI). Seventy adult patients were included in this prospective study. On the basis of the preoperative evaluation, 39 patients were clinically considered to have a PJI, whereas 31 were presumed to suffer from an aseptic mobilization of the implant. Xpert MRSA/SA SSTI identified 4 out of 4 MRSA, 7 out of 7 MSSA, and 14 out of 16 methicillin resistant CoNS...
March 1, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28253302/feasibility-of-the-tbdx-automated-digital-microscopy-system-for-the-diagnosis-of-pulmonary-tuberculosis
#16
Pamela Nabeta, Joshua Havumaki, Dang Thi Minh Ha, Tatiana Caceres, Pham Thu Hang, Jimena Collantes, Nguyen Thi Ngoc Lan, Eduardo Gotuzzo, Claudia M Denkinger
BACKGROUND: Improved and affordable diagnostic or triage tests are urgently needed at the microscopy centre level. Automated digital microscopy has the potential to overcome issues related to conventional microscopy, including training time requirement and inconsistencies in results interpretation. METHODS: For this blinded prospective study, sputum samples were collected from adults with presumptive pulmonary tuberculosis in Lima, Peru and Ho Chi Minh City, Vietnam...
2017: PloS One
https://www.readbyqxmd.com/read/28249999/utility-of-second-generation-line-probe-assay-hain-mtbdrplus-directly-on-2-month-sputa-specimens-to-monitor-tuberculosis-treatment-response
#17
Neesha Rockwood, Justyna Wojno, Yonas Ghebrekristos, Mark P Nicol, Graeme Meintjes, Robert J Wilkinson
The utility of line probe assay (Genotype MTBDRplus) performed directly on 2-month sputa to monitor tuberculosis treatment response is unknown. We assessed if direct testing of 2-month sputa with MTBDRplus can predict 2-month culture conversion and long-term treatment outcome. Xpert® MTB/RIF-confirmed rifampicin-susceptible tuberculosis cases were recruited at tuberculosis diagnosis and followed up at 2 and 5-6 months. MTBDRplus was performed directly on 2-month sputa and on all positive culture isolates at 2 and 5-6 months...
March 1, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28238388/multicenter-evaluation-of-the-revised-rida%C3%A2-quick-test-n1402-for-rapid-detection-of-norovirus-in-a-diagnostic-laboratory-setting
#18
Stijn Jonckheere, Nadine Botteldoorn, Patricia Vandecandelaere, Johan Frans, Wim Laffut, Guy Coppens, Anne Vankeerberghen, Hans De Beenhouwer
The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA® QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR®Green RT-rtPCR, but not by the RIDA® GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid)...
February 12, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28237624/three-year-evaluation-of-xpert-mtb-rif-in-a-low-prevalence-tuberculosis-setting-a-scottish-perspective
#19
Benjamin J Parcell, Anna Jarchow-MacDonald, Amie-Louise Seagar, Ian Laurenson, Gordon J Prescott, Michael Lockhart
OBJECTIVES: Xpert MTB/RIF (Cepheid) is a rapid molecular assay shown to be sensitive and specific for pulmonary tuberculosis (TB) diagnosis in highly endemic countries. We evaluated its diagnostic performance in a low TB prevalence setting, examined rifampicin resistance detection and quantitative capabilities predicting graded auramine microscopy and time to positivity (TTP) of culture. METHODS: Xpert MTB/RIF was used to test respiratory samples over a 3 year period...
February 22, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28229100/diagnosis-of-tuberculosis-following-world-health-organization-recommended-criteria-in-severely-malnourished-children-presenting-with-pneumonia
#20
Mohammod Jobayer Chisti, Mohammed Abdus Salam, Abu S M S B Shahid, K M Shahunja, Sumon Kumar Das, Abu Syed Golam Faruque, Pradip Kumar Bardhan, Tahmeed Ahmed
Evidences on diagnosis of tuberculosis (TB) following the World Health Organization (WHO) criteria in children with severe acute malnutrition (SAM) are lacking. We sought to evaluate the WHO criteria for the diagnosis of TB in such children. In this prospective study, we enrolled SAM children aged <5 with radiological pneumonia. We collected induced sputum and gastric lavage for smear microscopy, mycobacterial culture, and Xpert MTB/RIF. Using the last 2 methods as the gold standard, we determined sensitivity, specificity, and positive and negative predictive values of WHO criteria (n = 388)...
2017: Global Pediatric Health
keyword
keyword
26454
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"